Biomedical materials (Bristol, England)最新文献

{"title":"Thermal-crosslinked acellular dermal matrix combined with adipose-derived stem cells to regenerate vascularized adipose tissue.","authors":"Xing Huang, Siyuan Chen, Lin Lu, Rui Jin, Mengling Chang, Zhaoqi Yuan, Xusong Luo, Zhu Zhu, Guangpeng Liu","doi":"10.1088/1748-605X/adaff8","DOIUrl":"10.1088/1748-605X/adaff8","url":null,"abstract":"<p><p>The reconstruction of large-sized soft tissue defects remains a substantial clinical challenge, with adipose tissue engineering emerging as a promising solution. The acellular dermal matrix (ADM), known for its intricate spatial arrangement and active cytokine involvement, is widely employed as a scaffold in soft tissue engineering. Since ADM shares high similarity with decellularized adipose matrix, it holds potential as a substitute for adipose tissue. This study explores the adipogenic ability of a spongy material derived from ADM via vacuum-thermal crosslinking (T-ADM), characterized by high porosity, adjustable thickness, and suitable mechanical strength. Adipose-derived stem cells (ADSCs) are considered ideal seed cells in adipose tissue engineering. Nevertheless, whether pre-adipogenic induction is necessary before their incorporation remains debatable. In this context, ADSCs, both with and without pre-adipogenic induction, were seeded into T-ADM to regenerate vascularized adipose tissue. A comparative analysis of the two constructs was performed to evaluate angiogenesis and adipogenesis<i>in vitro</i>, and tissue regeneration efficacy<i>in vivo</i>. Additionally, RNA-seq analysis was utilized to investigate the potential mechanisms. The results showed that T-ADM exhibited good performance in terms of volume retention and maintenance of adipocyte phenotype, confirming its suitability as a scaffold for adipose tissue engineering.<i>In-vitro</i>outcomes demonstrated that pre-adipogenic induction enhanced the adipogenic level of ADSCs, but reduced their ability to promote vascularization. Furthermore, constructs utilizing pre-induced ADSCs showed an insignificant superiority in<i>in-vivo</i>fat formation, and neovascularization compared with those with non-induced ADSCs, which may be attributed to similar macrophage regulation, and balanced modulation of the proliferator-activated receptor-<i>γ</i>and hypoxia-inducible factor 1<i>α</i>pathways. Consequently, the direct use of ADSCs is advocated to streamline the engineering process and reduce associated costs. The combined strategy of T-ADM with ADSCs proves to be feasible, convenient and effective, offering substantial potential for addressing large-sized tissue deficits and facilitating clinical applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-aging chitosan/gelatin film crosslinked by<i>α</i>-arbutin for bone regeneration by free radical scavenging to prevent osteoblast senescence.","authors":"Guojie Xu, Lian Xi, Xiaohan Huang, Qingtiao Xie, Jinmin Zhao, Xianfang Jiang, Zhenhui Lu, Li Zheng","doi":"10.1088/1748-605X/adae6d","DOIUrl":"10.1088/1748-605X/adae6d","url":null,"abstract":"<p><p>Osteoblasts play a critical role in maintaining bone homeostasis. Senescence causes by free radical-mediated oxidative stress may affect the viability and osteogenic differentiation potential of osteoblast during bone formation. To eliminate the impacts of senescent cells by free radical scavenging is an optimal option for bone regeneration in age-related bone disease, such as osteoporosis (OP) and periodontitis. In this study, we fabricated an antioxidant film (CG-ARB) by crosslinking chitosan (C) and gelatin (G) using<i>α</i>-Arbutin (ARB) as a crosslinker. The morphological, physicochemical, and radical scavenging characteristics of the films were investigated. Its antioxidative ability to prevent osteoblast senescence for restoration of osteogenic differentiation was analyzed<i>in vitro</i>. A Sprague-Dawley rat model with critical size calvarial defect was used to evaluate the bone regeneration and biosafety<i>in vivo</i>. The results demonstrated that CG-ARB formed a dense fiber membrane, allowing for the gradual and sustained release of ARB for at least 10 d. ARB exerted antioxidant effect that prevented osteoblast senescence<i>in vitro</i>and promote bone healing<i>in vivo</i>. Furthermore, CG-ARB did not cause hemolysis or organ toxicity, and was therefore, considered biosafe. These results indicated that CG-ARB film could be an ideal drug delivery system for sustained released of ARB in bone defect repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferritin nanoparticles: new strategies for the diagnosis and treatment of central nervous system diseases.","authors":"Tao Guo, Muhammad Abid Hayat, Jiabo Hu","doi":"10.1088/1748-605X/adab5a","DOIUrl":"10.1088/1748-605X/adab5a","url":null,"abstract":"<p><p>Ferritin nanoparticles, which can penetrate the blood-brain barrier (BBB), have gained significant research interest for the diagnosis and treatment of central nervous system (CNS) diseases, including gliomas, Alzheimer's disease, and brain metastases. In recent years, ferritin has been proved as a candidate to cross the BBB using receptor-mediated transport (RMT) mechanism through transferrin receptor 1 (TfR1) which is overexpressed in the cells of the BBB. Various types of cargo molecules, including therapeutics, imaging agents, nucleic acids, and metal nanoparticles, have been incorporated into ferritin nanocages for the diagnosis and treatment of CNS diseases. In particular, low immunogenicity of ferritin implies safety for its usage in clinical practices, and high biocompatibility add to the perspectives of its applications. Furthermore, contemporary strides in molecular biology have enabled some alteration in the configuration of the ferritin outer layers and surface characters so as to enhance the drug encapsulation capacity and conjugation affinity. Such modifications not only enhance the property of ferritin in crossing the BBB, but also enhance its efficacy when applied to CNS diseases. In summary, ferritin, as a drug delivery system, shows great potential for the treatment and diagnosis of CNS diseases.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A recent study of natural hydrogels: improving mechanical properties for biomedical applications.","authors":"Atharva Shukla, Putri Hawa Syaifie, Syahnanda Jaya, Nurul Taufiqu Rochman, Muhammad Miftah Jauhar, Etik Mardliyati","doi":"10.1088/1748-605X/adb2cd","DOIUrl":"https://doi.org/10.1088/1748-605X/adb2cd","url":null,"abstract":"<p><p>Natural polymer-based hydrogels, generally composed of hydrophilic polymers capable of absorbing large amounts of water, have garnered attention for biomedical applications because of their biocompatibility, biodegradability, and eco-friendliness. Natural polymer-based hydrogels derived from alginate, starch, cellulose, and chitosan are particularly valuable in fields such as drug delivery, wound dressing, and tissue engineering. However, compared with synthetic hydrogels, their poor mechanical properties limit their use in load-bearing applications. This review explores recent advancements in the enhancement of the mechanical strength of natural hydrogels while maintaining their biocompatibility for biomedical applications. Strategies such as chemical modification, blending with stronger materials, and optimized cross-linking are discussed. By improving their mechanical resilience, natural hydrogels can become more suitable for demanding biomedical applications, like tissue scaffolding and cartilage repair. Additionally, this review identifies the ongoing challenges and future directions for maximizing the potential of natural polymer-based hydrogels in advanced medical therapies.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradable Mg-1%Ca alloy inhibits the growth of cervical cancer.","authors":"Yunshan Ouyang, Lingling Cao, Qian Zhao, Wang Yang, Chen Lin","doi":"10.1088/1748-605X/adb2cc","DOIUrl":"https://doi.org/10.1088/1748-605X/adb2cc","url":null,"abstract":"<p><p>The traditional treatment for cervical cancer involves aggressive surgery combined with radiotherapy and chemotherapy. Nevertheless, these treatments have certain limitations and side effects, thus breakthroughs and advances are required in cervical cancer therapy. Magnesium alloy is a promising antitumor biomaterial with excellent biocompatibility and biodegradability. However, the potential effects of magnesium alloy on cervical tumors have not been extensively explored. Recent studies have demonstrated that adding a small amount of calcium to the magnesium matrix can reduce grain size and corrosion rate while providing good biocompatibility. We conducted in vivo and in vitro experiments to test the antitumor properties of Mg-1%Ca alloys. The results indicated that the Mg-1%Ca alloy released Mg2+ and OH- more slowly, inhibited the proliferation of SiHa and HeLa cells, induced apoptosis in tumor cells, disrupted the cytoskeleton, and inhibited cell migration and invasion. At the molecular level, Mg-1%Ca alloy&#xD;the MAPK/ERK signaling pathway. In the future, Mg-1%Ca may be employed in the treatment of cervical cancer as a novel adjuvant therapeutic material with anticancer function to prevent the occurrence and progression of cancer proliferation and metastasis.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed polycaprolactone/poly (L-lactide-co-ε-caprolactone) composite ureteral stent with biodegradable and antibacterial properties.","authors":"Yanjiao Teng, Xinyan Wang, Lin Song, Jianing Yang, Shike Hou, Qi Lv, Li Jiang, Yong Guan, Jie Shi","doi":"10.1088/1748-605X/adb2ce","DOIUrl":"https://doi.org/10.1088/1748-605X/adb2ce","url":null,"abstract":"<p><p>The clinical application of biodegradable ureteral stents holds significant potential. There is an urgent need to develop new materials for ureteral stents to address the limitations related to performance degradation and antibacterial properties observed in current designs. Here, we developed a Polycaprolactone (PCL)/Poly (L-lactide-co-ε-caprolactone) (PLCL) composite ureteral stent by 3D printing, which exhibits biodegradable and antibacterial properties. Silver nanoparticles (AgNPs) were bonded to the surface of the stent through the polymerization of dopamine (PDA) and coating with type I collagen (Col I). The ureteral stent (PP-PDA-Ag-Col) had a densely spiraled structure and higher hydrophilicity. The release behavior of silver ions from the stent was found to be slow and continuous when coated with AgNPs, which can enable long-term antibacterial effects after being implanted in vivo. Additionally, in vitro degradation experiments demonstrated that the different ratios of ureteral stents degraded slowly in artificial urine over 6 weeks without compromising functionality. The stent exhibits excellent hemocompatibility and cell compatibility. The subcutaneous implantation experiment in SD rats showed that the PP-PDA-Ag-Col stent degraded slowly in vivo and had good biocompatibility. The stent PCL5/PLCL5 was the most promising ureteral stent regarding antibacterial, mechanical properties, and degradation. The novel 3D-printed PP-PDA-Ag-Col stent exhibits biocompatibility for safe in vivo transplantation and antibacterial properties that reduce reliance on antibiotics. Additionally, its biodegradability eliminates the need for secondary surgical removal, making it a promising option for the clinical application of ureteral stents.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan hydrochloride coated and nonionic surfactant modified niosomes: a better way for oral administration of semaglutide.","authors":"Ben Wang, Zhengxing Su, Meiyan Kuang, Yi Luo, Minhao Xu, Meng Sun, Xingyou Liu, Yue Guo, Lu Bai, Yu Wang, Xinlei Yan, Jing Xie, Yaqin Tang","doi":"10.1088/1748-605X/adb2cf","DOIUrl":"https://doi.org/10.1088/1748-605X/adb2cf","url":null,"abstract":"<p><p>Diabetes is now a global chronic disease, with the number of people with diabetes expected to reach 643 million by the end of 2030. Semaglutide, a human glucagon-like peptide-1 (GLP-1) analogue with 94% similarity to human GLP-1, can promote insulin secretion and repress glucagon secretion in a glucose concentration-dependent manner, resulting in substantial improvement of blood glucose levels and reducing the risk of hypoglycemia in patients with type 2 diabetes. To improve the absorption efficiency of semaglutide in oral delivery, we developed chitosan hydrochloride-coated and nonionic surfactant-modified niosomes (CS.HCL-NSPEs-NIO) as a new way to encapsulate it. The results showed that CS.HCL-NSPEs-NIO could efficiently penetrate the cell junctions in the intestinal endothelium and therefore promote drug absorbance. In addition, gastrointestinal distribution studies revealed that CS. HCL-NSPEs-NIO could stay in the intestine for more than 4 hours, thus allowing for long-term glucose regulation. Effective reduction of blood glucose levels and weight loss were observed in db/db mice while no toxicity was detected in major organs. Overall, we suggest CS.HCL-NSPEs-NIO is a promising oral delivery agent to improve the hypoglycemic effect of semaglutide.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Decellularization of precision-cut kidney slices-application of physical and chemical methods (2023<i>Biomed. Mater.</i>18 025004).","authors":"Haitham Salti, Lea Kramer, Sophie-Charlotte Nelz, Mathias Lorenz, Anne Breitrück, Jacqueline Hofrichter, Marcus Frank, Karoline Schulz, Steffen Mitzner, Reinhold Wasserkort","doi":"10.1088/1748-605X/adae47","DOIUrl":"https://doi.org/10.1088/1748-605X/adae47","url":null,"abstract":"","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In situ</i>swelling of low-friction, high load-bearing self-bending bilayer hydrogels inspired by articular cartilage.","authors":"Jianfeng Chen, Chuan Li, Xiaoxiao Chen, Kui Zhou, Hanjing Li, Kai Peng, Yinong Yang, Yichuan Dai, Ben Huang","doi":"10.1088/1748-605X/ada7b4","DOIUrl":"10.1088/1748-605X/ada7b4","url":null,"abstract":"<p><p>The articular cartilage is characterized by its gradient hierarchical structure, which exhibits excellent lubrication and robust load-bearing properties. However, its inherent difficulty in self-repair after damage presents numerous formidable challenges for cartilage repair. Inspired by the unique structure of articular cartilage, a biomimetic bilayer hydrogel composed of PAM (polyacrylamide) and PAM/SA (sodium alginate) is prepared using a two-step<i>in-situ</i>swelling method. The bilayer hydrogel demonstrates exceptional structural stability due to the interlayer<i>in-situ</i>chemical cross-linking. Compared to monolayer hydrogels, the PAM-PAM/SA bilayer hydrogel demonstrates superior mechanical attributes, exhibiting a compressive strength of 1 MPa and a compressive modulus of 0.22 MPa. Furthermore, exploration of the tribological performance of the PAM-PAM/SA bilayer hydrogel have revealed its low-friction performance under high loads, with a coefficient of friction as low as 0.032. Finally, leveraging the differential swelling properties between the distinct layers of the PAM-PAM/SA bilayer hydrogel, a self-bending biomimetic cartilage capable of conforming to complex joint surfaces is fabricated. This highly lubricating, mechanically robust, and conformal biomimetic cartilage provides an effective means for addressing cartilage defects and joint diseases.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and optimization of physical, mechanical, and biological characteristics of 3D printed Whitlockite/calcium silicate composite scaffold for bone tissue regeneration using response surface methodology.","authors":"Mahendran Thangavel, Renold Elsen S","doi":"10.1088/1748-605X/adad27","DOIUrl":"10.1088/1748-605X/adad27","url":null,"abstract":"<p><p>Calcium phosphate-based bioscaffolds are used for bone tissue regeneration because of their physical and chemical resemblance to human bone. Calcium, phosphate, sodium, potassium, magnesium, and silicon are important components of human bone. The successful biomimicking of human bone characteristics involves incorporating all the human bone elements into the scaffold material. In this work, Mg-Whitlockite (WH) and Calcium Silicate (CS) were selected as matrix and reinforcement respectively, because of their desirable elemental composition and regenerative properties. The magnesium in WH increases mineralization in bone, and the silicon ions in CS support vascularization. The Mg-WH was synthesized using the wet chemical method, and powder characterization tests were performed. Response surface methodology (RSM) is used to design the experiments with a combination of material compositions, infill ratios (IFs), and sintering temperatures (STs). The WH/CS bioceramic composite is 3D printed in three different compositions: 100/0, 75/25, and 50/50 wt%, with IFs of 50%, 75%, and 100%. The physical and mechanical characterization study of printed samples is conducted and the result is optimized using RSM. ANOVA (Analysis of Variance) is used to establish the relationship between input parameters and responses. The optimized input parameters were the WH/CS composition of 50/50 wt%, IF of 50%, and ST of 1150 °C, which bring out the best possible combination of physical and mechanical characteristics. The RSM optimized response was a density of 2.27 g cm^-3, porosity of 36.74%, wettability of 45.79%, shrinkage of 25.13%, compressive strength of 12 MPa, and compressive modulus of 208.49 MPa with 92% desirability. The biological characterization studies were conducted for the scaffold samples prepared with optimized input parameters. The biological studies confirmed the capabilities of the WH/CS composite scaffolds in bone regenerative applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}