Biomedical materials (Bristol, England)最新文献

{"title":"Investigation of copper doped<i>β</i>-TCP and GB14 coatings produced via high velocity suspension flame spraying-a biocompatibility and antimicrobial activity study.","authors":"Long-Quan R V Le, Maria Carolina Lanzino, Anika Höppel, Hermann O Mayr, Ali Al-Ahmad, Bettina Spitzmüller, Moritz Mayr, Anna Baghnavi, Michael Seidenstuecker","doi":"10.1088/1748-605X/adda82","DOIUrl":"10.1088/1748-605X/adda82","url":null,"abstract":"<p><p>Implant-associated infections and aseptic loosening of prosthesis due to insufficient secondary stability continue to present a challenging issue in arthroplasty. Potential solutions include bioactive coatings to promote osseointegration. With this in mind, this study aims to investigate and compare thin bioactive and bioresorbable<i>β</i>-tricalcium phosphate (<i>β</i>-TCP) and calcium alka-li orthophosphate (GB14) coatings, both produced via high velocity suspension flame spraying. To achieve an additional antibacterial effect and to prevent infections through aerosolized contamination, Cu-doped<i>β</i>-TCP supraparticles (SP) are incorporated into the coatings.<i>β</i>-TCP and GB14 coatings with 0.5 wt.% Cu-doped<i>β</i>-TCP SP each were investigated. According to ISO EN 10993-14, a degradation test was performed in TRIS-buffer at pH 7.4 over 120 h. Biocompatibility testing was performed on human osteoblasts using live/dead staining on days 1, 3 and 7 to simultaneously visualize viable and non-viable cells, while cytotoxicity was assessed over a 3 d period with the cytotoxicity assay. To evaluate the antibacterial efficacy, safe airborne antibacterial assays using<i>S. aureus</i>and<i>E. coli</i>were performed. Our investigations demonstrate that Cu is released from the coatings over a period of 120 h. The released Cu-amount results in a significant reduction in colony forming units across all coatings, while only negligibly imparing the behavior of the human osteoblasts. Both coatings exhibit high biocompatibility, with cell counts varying depending on the amount of Cu released. Cytotoxicity testing showed no cytotoxic effects for the samples examined.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regeneration responses of Ankaferd Blood Stopper, platelet-rich-plasma, and<i>Momordica charantia</i>on sciatic nerve injury in the obese rats.","authors":"Mehmet Emin Önger, Stefano Geuna, Abubaker El Elhaj, Stefania Raimondo, Suleyman Kaplan","doi":"10.1088/1748-605X/add97c","DOIUrl":"10.1088/1748-605X/add97c","url":null,"abstract":"<p><p>Obesity is a prevalent and potentially fatal disorder in industrialized nations, often due to an imbalance between calorie intake and energy expenditure. The study investigates the effects of Ankaferd Blood Stopper (ABS), platelet-rich plasma (PRP), and<i>Momordica charantia</i>(MC) on obese rats with sciatic nerve injuries using stereological and electron microscopic techniques. Twenty-four female Sprague Dawley rats, aged 8-10 weeks, were divided into three groups: the obese gap ABS group (OGABS), the obese gap PRP group (OGPRP), and the obese gap MC group (OGMC). A five-mm nerve block was resected approximately 10 mm above the nerve branch. The gap region was then surrounded and closed by a collagen membrane in tube form. Materials such as ABS, PRP, and MC were injected into the tubes of each group. Electromyography and histological procedures were performed after 12 weeks of surgery to investigate their structural repair and functional promotion of nerve regeneration. A significant increase in the number of myelinated axons in OGMC was found. In addition, the latency values in OGMC were more critical than in the OGABS and OGPRP groups, indicating better myelination. PRP and ABS positively affected nerve gap repair, favoring regenerating unmyelinated axons. Due to increased amplitude values, PRP and ABS have also improved axonal area regeneration. Collagen tubes containing ABS, PRP, and MC may help repair and close peripheral nerve gaps in obese rats, which could be beneficial in closing peripheral nerve resection and demyelination. The study's findings indicate that these collagen tubes can have a therapeutic effect on peripheral nerve regeneration in obese models.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144082487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fib@PEGDA/GelMA Hydrogel as a Light-curing Thin-layer Matrix for RPE Cell Growth and Function.","authors":"Naiwen Zhang, Cong Ma, Fei Shao, Huanan Wang, Xiang Ma","doi":"10.1088/1748-605X/addcab","DOIUrl":"https://doi.org/10.1088/1748-605X/addcab","url":null,"abstract":"<p><p>Retinal degenerative diseases, including age-related macular degeneration (AMD) and retinitis pigmentosa (RP), are leading causes of blindness globally, characterized by progressive degeneration of retinal pigment epithelium (RPE) and photoreceptor (PR) cells. Despite advancements, current therapies have not substantially arrested disease progression. Cell replacement therapy using healthy RPE and PR cells holds promise but faces obstacles such as poor cell survival, inadequate integration, and transplantation difficulties. To address these issues, tissue engineering combined with 3D printing has become a focal point. This study investigates the use of four hydrogels-GelMA, HAMA, AlgMA, and PEGDA-and their various crosslinked combinations for creating hydrogel thin-layer matrices conducive to RPE cell growth. PEGDA/GelMA hydrogel demonstrated optimal support for cell spreading and proliferation, which is not achievable with hydrogels matrices of other formulations. The relationship between the mechanical properties of PEGDA/GelMA hydrogels and cell growth was further refined. PEGDA600-20 hydrogel with a compressive modulus of 1245.07 ± 20.79 kPa was selected based on time-course viability assays, leading to the development of the optimized Fib@PEGDA/GelMA hydrogel exhibited exceptional biocompatibility. Compared to PEGDA/GelMA, CCK-8 assays demonstrated significantly improved relative cell viability at 24 h, 48 h, and 72 h, with increases of 17.73 ± 1.22%, 14.54 ± 3.63%, and 19.04 ± 2.31%, respectively on Fib@PEGDA/GelMA matrix. qRT-PCR results indicated a mitigation of epithelial-mesenchymal transition (EMT), as evidenced by downregulation of EMT markers (CDH2, COL1A1, and FN1), accompanied by reduced IL-6 levels. Fib@PEGDA/GelMA hydrogel enhanced phagocytic activity in ARPE-19 cells and promoted functional expression in hiPSC-RPEs. Additionally, the hydrogel showed favorable in vivo biocompatibility following subcutaneous implantation of RCS rats at 1, 2, and 4 weeks post-implantation evidenced by HE and Masson's staining. This system offers a promising bioink for 3D-printed retinal cell scaffolds and paves the way for future advancements in cell replacement therapies for retinal degenerative diseases.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of sea cucumber protein paste for mice's skin wound healing and its potential anti-inflammatory mechanism.","authors":"Xiaoyan Wang, Jinghe Sun, Ke Liu, Shuang Li, Jun Zhao, Jingfeng Yang","doi":"10.1088/1748-605X/addcaa","DOIUrl":"https://doi.org/10.1088/1748-605X/addcaa","url":null,"abstract":"<p><p>Natural substances with anti-inflammatory activity have always been the priority for human injuries. This study aims to investigate the beneficial effects and mechanism of sea cucumber protein (SCP) on wound healing, through a BALB/c mice model and LPS-induced RAW 264.7 cells. To find out how SCP paste works, we identified the mice's serum cytokines and tissue section. The alteration of the NF-κB pathway during the anti-inflammatory effect of SCP was also explored. The results showed that the wound healing rate in the SCP(H) group exceeded 90%, whereas it was 72.91% and 64.10% in the Control and NC groups on day 14. New blood vessels and fibroblasts were generated in the wounds. Collagen expression increased by 13.89% and 15.12% respectively in the SCP(L) and SCP(H) groups compared with the Control group on day 14. Furthermore, SCP decreased the levels of pro-inflammatory factors (TNF-α, IL-1β, IL-6) in mice's serum while up-regulating the level of anti-inflammatory factor (IL-10) during the healing process. Furthermore, SCP suppressed the NF-κB pathway by decreasing protein levels of phosphorylated p65 and IKKα, and increasing protein levels of IκBα.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel innovations for 3D organoid culture.","authors":"Yicheng Feng, Dongyang He, Xiao An","doi":"10.1088/1748-605X/add82d","DOIUrl":"10.1088/1748-605X/add82d","url":null,"abstract":"<p><p>Organoids are functional cell-tissue complexes that mimic structural and functional characteristics of organs<i>in vitro</i>in three dimensions (3D). Mimicking the natural extracellular matrix (ECM) environment is critical for guiding stem cell fate within organoid cultures. Current organoid cultures predominantly utilize animal- or tumor-derived ECMs such as dECMs and Matrigel. However, these materials introduce batch variability and uncertainty in composition, which hinders reproducibility. In contrast, naturally derived and synthetic hydrogels with excellent biocompatibility offer precise and adjustable compositions, along with tunable mechanical properties, thereby providing robust support for organoid development and maturation. We explore innovative hydrogel designs tailored specifically for organoid cultures, emphasizing the influence and meticulous control of functional hydrogels on organoid formation, differentiation, and maturation processes. Furthermore, the review highlights the potential of functionalized hydrogel scaffolds to advance both research and industrial applications in tissue and organ engineering. As research progresses, investigations will further concentrate on improving the adjustable properties, expanding their scope of application, and more biologically compatible gelation strategies of hydrogels.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapidly curable zinc chondroitin sulfate @ methacrylated hyaluronic acid hydrogel: a novel photocurable biomaterial tailored for emergency wound management.","authors":"Shiman Li, Qili Sun, Huicheng Cao, Chunan Lu, Jialin Yu, Kangyu Chen, Chuqing Tang, Zimo Li, Shuaishuai Cao, Tenghui Zeng, Bin Tang","doi":"10.1088/1748-605X/add63b","DOIUrl":"10.1088/1748-605X/add63b","url":null,"abstract":"<p><p>In today's emergency medical field, rapid hemostasis and wound healing technologies are of paramount importance. However, traditional methods, although effective, have limitations such as slow hemostasis, susceptibility to infection, and unsuitability for irregular wounds. To address these issues, this study combined methacrylated hyaluronic acid (HAMA) with zinc chondroitin sulfate (CSZn) to successfully develop a novel sprayable photocurable hydrogel, CSZn@HAMA. Material characterization confirmed that CSZn was effectively loaded into HAMA, while retaining HAMA's photocurable and sprayable properties. This allows the CSZn@HAMA hydrogel to rapidly solidify and form a tight protective film over the wound after spraying. Further cell experiments demonstrated that this hydrogel has significant anti-inflammatory effects and can effectively promote collagen production and angiogenesis. Therefore, CSZn@HAMA has emerged as a promising biomaterial for wound management in emergency medical care.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and processing of physical properties of anti-calcified glutaraldehyde-treated three-dimensional cultured cartilage tissues.","authors":"Yohei Kitaguchi, Tomoyuki Ota, Tomoka Takao, Ryosuke Iwai, Takeshi Moriwaki, Yuki Fujisawa, Daisuke Yamada, Takeshi Takarada","doi":"10.1088/1748-605X/addbb5","DOIUrl":"https://doi.org/10.1088/1748-605X/addbb5","url":null,"abstract":"<p><p>Reconstruction using cartilage tissue is necessary to address deformities of the nose, ears, and maxillofacial region in several cases. However, autologous cartilage tissue transplantation is limited in the amount that can be harvested owing to invasiveness to the human body.&#xD;Moreover, artificial materials such as implants cannot be used in many situations, given their potential to induce reactions to foreign bodies. Therefore, there is a growing demand for biomaterials that are less likely to cause foreign body reactions. Given that a tissue with a functionally superior three-dimensional structure can replace autologous tissue and artificial materials, we have developed a three-dimensional cultured cartilage tissue without scaffolding material and are working toward its practical application. To achieve an off-theshelf product that allows prolonged storage, the tissue was fixed with glutaraldehyde to maintain high strength for subsequent processing and management. Although tissue fixation with glutaraldehyde may cause calcification due to the deposition of calcium phosphate, calcification can be prevented by washing with high-concentration ethanol. We generated three-dimensional cultured cartilage tissues using induced pluripotent stem cell-derived limb bud mesenchymal cells and an original cell self-culture aggregation method. The generated tissues were subjected to an anti-calcification treatment with glutaraldehyde and 80% ethanol.&#xD;The treated tissue had improved stability and strength with minimal calcification. The tissue retained its physical properties that were effectively processable and could be processed into an ear-like shape.&#xD;&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual drug-loaded self-wearable electrospun nanofiber for synergistic pharmacological intervention through tertiary hemostasis in prehospital trauma care.","authors":"Pranabesh Kumar Sasmal, Shalini Dasgupta, Sujankrishna Samanta, Samsamul Hoque, Kolimi Prashanth Reddy, Samit Kumar Nandi, Abhijit Chanda, Pallab Datta","doi":"10.1088/1748-605X/addbb6","DOIUrl":"https://doi.org/10.1088/1748-605X/addbb6","url":null,"abstract":"<p><p>Uncontrolled bleeding is a critical concern in both wartime and civilian trauma emergencies. Current mechanical hemostatic patches do not always suffice while not addressing rebleeding often observed during patient transportation. The unmet clinical need has led to exploring drug loaded hemostat dressings, providing mechanical hemostasis and drug delivery to the bleeding site to stabilize clots. In the present study, hemostatic nanofiber patches (PVA/CS/TXA-E) were prepared by taking a combination of chitosan, polyvinyl alcohol, with two different hemostatic drugs namely tranexamic acid, and ethamsylate to exert a synergistic pharmacological augmentation of hemostat performance of the nanofibers. The developed nanofiber patches comprised of 400 nm diameter and had 366-459% swellability. The nanofiber possessed intermittent hydrophilicity (water contact angle 32). Drug release through the nanofiber followed a non-Fickian diffusion model. Cytocompatibility (80% and above) and hemocompatibility (less than 8%) of the patches was established. The haemorrhage control capacity was studied in vitro and ex vivo on rabbit skin. Dual drug loading decreased the clotting time by 24%, while aPTT, PT and PRT was also decreased by 6%, 20% & 15% over either drug loaded nanofibers. In vivo results corroborated the hemostat performance and evidence presence of granularity indicative of wound healing progression. Our results suggest PVA/CS/TXA-E potential as a highly effective hemostatic nanofiber with biocompatibility for managing hemorrhage and facilitating wound healing post-surgery.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xanthan gum-based protocatechuic acid grafted carboxymethyl chitosan hydrogel with injectable, spraying, self-healing, and enhanced antioxidant properties.","authors":"Jiaxue Tang, Chuzhou Wen, Shengnan Zheng, Changkai Sun, Fengtao Wang, Shui Guan","doi":"10.1088/1748-605X/addb1d","DOIUrl":"https://doi.org/10.1088/1748-605X/addb1d","url":null,"abstract":"<p><p>Overcoming the limitations of conventional antioxidants in treating oxidative stress-related neurodegenerative diseases remains a critical challenge, thus more effective antioxidant strategies need to be studied urgently. To address this, we developed a novel pH-responsive drug-delivery hydrogel, PCA-g-CMCS/OXG, by grafting protocatechuic acid (PCA) onto carboxymethyl chitosan (CMCS) via amide bonds and blending it with oxidized xanthan gum (OXG) to form dynamic imine bonds. The conjugate PCA-g-CMCS achieved an unprecedented grafting efficiency of 785.3 mg/g through optimized reactant ratios, pH, and reaction time. And the multifunctional hydrogel PCA-g-CMCS/OXG offers three key advantages: (1) rapid tunable gelation time (10-110 s) and robust mechanical/rheological properties enabling injectable and sprayable applications; (2) self-healing capability and sustained pH-responsive PCA release over 15 days, ensuring long-term therapeutic efficacy; and (3) superior cytoprotection, as the hydrogel exhibited excellent biocompatibility with SH-SY5Y neuronal cells and significantly increased cell viability to 76.60% from H2O2-induced oxidative damage (vs. 48.61% for control, p < 0.01). Therefore, the smart Schiff's base hydrogel is a drug loaded material with great clinical application prospect for the treatment of neurodegenerative diseases.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dressing antibacterial platinum loaded polyurethane materials by a stable hydrophilic coating with robust antithrombotic properties.","authors":"Shuai Gao, Zheng Liu, Wei Zeng, Xi Liu, Fanjun Zhang, Dimeng Wu, Yunbing Wang","doi":"10.1088/1748-605X/add6fa","DOIUrl":"10.1088/1748-605X/add6fa","url":null,"abstract":"<p><p>Thrombus formation and infection resulting from blood contact with medical materials represent significant clinical complications characterized by a mutually reinforcing relationship between the two phenomena. Consequently, the development of hydrophilic coatings that simultaneously release bactericidal agents and exhibit passive antithrombotic properties is of paramount importance. In this work, we employed a straightforward, easily executable, and amenable to scale-up strategy to synthesize an antibacterial polyurethane matrix containing platinum complex, followed by the construction of a phosphorylcholine-based hydrophilic coating on its surface via surface-initiated polymerization. The antibacterial efficacy arises from the sustained release mechanism of platinum complex ions, while the phosphorylcholine coating exhibits remarkable antithrombotic characteristics. These two functionalities operate both independently and synergistically within the material to provide robust antibacterial and antithrombotic performance.<i>In vitro</i>and<i>in vivo</i>experiments validated the effectiveness of this composite material in inhibiting bacterial growth and preventing thrombus formation. Furthermore, histological analysis along with immunological detection confirmed that the coating material has excellent biocompatibility coupled with anti-inflammatory effects. Therefore, the developed innovative coating with efficient antibacterial and antithrombotic effects shows great promise as an effective strategy for mitigating risks associated with infections and thrombus formation in clinical settings involving blood-contacting medical devices and related implants.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}