Biomedical materials (Bristol, England)最新文献

{"title":"Cytotoxicity mitigated silica entrapped copper doped Zinc Sulfide Quantum dots as luminescent nanoprobes for biolabeling.","authors":"Remya Muralimanohar, S Saravana Kumar","doi":"10.1088/1748-605X/adc230","DOIUrl":"10.1088/1748-605X/adc230","url":null,"abstract":"<p><p>Quantum dots (QDs) are luminescent semiconductor nanoparticles with unique optical properties that facilitate their use in sensing, biological labeling, optical imaging, and diagnostics. Wider band gap materials, such as Zinc sulfide, are extensively employed as QD nanoprobes since they offer higher photostability, higher quantum yield, larger molar extinction coefficients, and longer fluorescence lifetimes than conventional organic fluorescent dyes used in bioassays. Tunable multiphoton emission in QDs is accomplished by doping with transition metals, of which, copper is the most beneficial owing to its comparable ionic radius, intense emission, and composition-variable spectral broadening. However, an overdose of Cu is toxic to the cells, leading to apoptosis. This cytotoxicity impedes the utilization of Cu-doped ZnS QDs for biolabeling. The present work deals with the diminution of copper cytotoxicity in Cu-doped ZnS Q-dots by means of silica entrapment, equipping them for<i>in vitro</i>and<i>in vivo</i>bioassays in the future. Cu-doped ZnS Q-dots were synthesized by chemical precipitation method and overlaid with silica by sol-gel method. Cytotoxicity investigation was performed on L929 Mouse fibroblast cells. X-ray diffraction studies confirmed that the prepared Q-dots were approximately 2 nm in size and were in the cubic phase. High resolution transmission electron microscopy revealed the spherical morphology of Q-dots. Micro-Raman Analysis was used to determine the Raman modes of the samples. Band gap energy was computed using UV-Visible Spectroscopy. Photoluminescence (PL) Spectroscopy demonstrated two emission peaks around 418 nm and 455 nm due to sulfur vacancy and copper trap levels, respectively, for Cu:ZnS Q-dots with hiked PL intensity on silica coating.<i>In vitro</i>cell toxicity studies performed on the as-prepared Q-dots by microscopic observation of treated cells, as well as by MTT colorimetric assay, manifested the attenuation of cytotoxicity in silica overspread copper-doped Q-dots. Silica entrapment subsided the copper-induced cytotoxicity by minimizing the photochemical oxidation of the Q-dots surface together with making them hydrophilic. Furthermore, silica coating boosted the PL intensity of the Q-dots. Such Q-dots could be a potent alternative to fluorescent organic pigments for biolabeling.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Floating or adherent hepatocyte spheroid cultures using microwell chips with polyethylene glycol or polyimide surfaces.","authors":"Sae Yokomine, Tomomi Makino, Emiko Nagao, Kohji Nakazawa","doi":"10.1088/1748-605X/adc17d","DOIUrl":"10.1088/1748-605X/adc17d","url":null,"abstract":"<p><p>Microwell chip culture is a promising technique for controlling spheroid size and producing a large number of homogeneous spheroids. In this study, we focused on the relationship between chip material and the properties of hepatocyte spheroids. The basic chip design was 397 circular microwells, each 400 µm in diameter. Two types of microwell chips were fabricated, coating the bottom surface either with polyethylene glycol (PEG chip) or polyimide (PI chip). Hepatocytes gradually aggregated and formed floating spheroids within each microwell in the PEG chip but formed adherent spheroids within each microwell of the PI chip. Such floating and adherent spheroid morphologies were maintained for at least one month of culture. An explanation for the spheroid formation mechanism is that the plasminogen activator (PA) /plasmin and matrix degradation/remodeling systems were activated in the formation of adherent spheroids. Furthermore, in adherent spheroid cultures, the formation of cell-matrix junctions was promoted, in addition to the development of intercellular junctions. The albumin secretion and drug metabolism activities of the hepatocyte spheroids were higher than those of traditional monolayer hepatocytes, and the adherent spheroids in the PI chip maintained a higher functional expression than the floating spheroids in the PEG chip. Further to this, functional properties of hepatocytes, the expressions of key metabolic enzymes, glucose 6-phosphatase (sugar metabolism), tryptophan 2, 3-dioxygenase (amino acid metabolism), arginase 1 (urea cycle), cytochrome P450 7a1 (lipid metabolism), and cytochrome P450 families (drug metabolism) were evaluated by gene expression analysis. The expression of these key enzymes in hepatocytes was higher in spheroid culture than in general monolayer culture, and the functions of adherent spheroids were superior to those of floating spheroids. These results indicate that the material properties of the microwell chips are important factors that regulate the morphological and functional characteristics of hepatocyte spheroids.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personalized assessment and monitoring of bone health from sweat: unveiling TEGO doped wearable, non-invasive hydrogel nanocomposite biosensor empowered by IL-6 detection.","authors":"Seema Rani, Sanchita Bandyopadhyay-Ghosh, Subrata Bandhu Ghosh","doi":"10.1088/1748-605X/adc05a","DOIUrl":"10.1088/1748-605X/adc05a","url":null,"abstract":"<p><p>Portable biosensing is crucial for rapid detection and continuous monitoring of bone diseases such as osteoporosis and bone cancer. It is well established that such bone disorders or diseases trigger release of inflammatory cytokines including interleukin-6 (IL6), detectable in sweat by electrochemical immunosensors. To this end, this study presents a novel hydrogel nanocomposite based immunosensor with highly conductive dual-layer of thermally exfoliated graphene oxide, toward precise detection and determination of loading level of IL-6 biomarker, and in turn, developing a label-free flexible bone biosensing platform. The immunosensor employed antibody immobilization process, which was further facilitated by the modification of the dual-layer by using 1-pyrenebutyric acid N-hydroxy succinimide ester. A thorough analysis of the effects of surface modification was conducted utilizing spectroscopic, electrochemical, and morphological methods. The biosensor's response was assessed through the utilization of the cyclic voltammetry measurement, which exhibited remarkable selectivity, achieving a low limit of detection of 15.4 pg ml^-1across a wide linear range. Additionally, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and Raman spectroscopy were successfully used to validate the sensing substrate in bio-fluidic samples and to understand the structure-property correlation. This innovative portable and flexible biosensor thus offers a practical and effective tool for potential application in continuous monitoring of bone health.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of novel antibacterial bio-adhesive for the treatment of periodontitis.","authors":"Yin Xu, Yixian Zhou, Shengjin Meng, Chengchao Zhou, Nannan Yang, Luhan Bao, Weizhong Lu","doi":"10.1088/1748-605X/adc6df","DOIUrl":"https://doi.org/10.1088/1748-605X/adc6df","url":null,"abstract":"<p><p>Periodontitis seriously affects people's daily health, and the development of a non-antibiotic bio-adhesive with antimicrobial and periodontitis regeneration for periodontal pockets will effectively promote the treatment of periodontitis. In this study, we constructed a hybrid hydrogel (GelMA-BC-PL) by introducing aldehyde bacterial cellulose (BC) short nanofibers into the photosensitive hydrogel (GelMA), which binds to periodontal tissues to play an adhesive role through the Schiff base reaction, and further introducing ε-polylysine (PL), which could achieve the adhesive, antibacterial, and regenerative effect. Pigskin adhesion experiments showed that the adhesion of GelMA hydrogel to pigskin was only 0.39 N, while that of GelMA-BC-PL reached 1.42 N. The adhesion performance of the hydrogel was significantly improved by adding aldehyde BC nanofibers. Due to the introduction of ε-polylysine, the antimicrobial properties of the hybrid hydrogel against two typical periodontitis bacteria (porphyromanas gingivalis and fusobacterium nucleatum), were significantly improved. Experiments with human periodontal membrane fibroblasts showed that the hybrid hydrogel had excellent cell spreading and proliferation promotion properties. The hybrid hydrogel simultaneously achieves adhesion, antimicrobial properties and promotes periodontal regeneration, which has great potential for application in the treatment of periodontitis diseases.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of PLGA microspheres loaded with natural drug tannins in tumor treatment.","authors":"Yiqiao Zhao, Yixuan Huang, Chaofan Chen, Chaoyu Cai, Xin Liu, Xiaohong Jiang, Aliaksandr Rahachou","doi":"10.1088/1748-605X/adc6d7","DOIUrl":"https://doi.org/10.1088/1748-605X/adc6d7","url":null,"abstract":"<p><p>Although conventional chemotherapeutic drugs exhibit broad-spectrum antitumor efficacy, their toxic effects on normal cells cannot be ignored, and there is an urgent need to develop novel drugs to improve therapeutic efficacy and reduce side effects. In this study, we prepared PLGA/TA microspheres with tannin as the target drug and PLGA as the carrier, and evaluated their physicochemical properties, including particle size, morphology, and release behavior, with the aim of exploring the potential of PLGA/TA microspheres for tumor therapy. The results of RNA sequencing showed that PLGA/TA microspheres may act on tumor cells mainly through the pathway of PI3K-Akt, and do not interfere with the synthesis of DNA directly. synthesis. Overall, PLGA/TA microspheres, as a novel drug delivery system, showed good anti-tumor potential and provided new ideas and directions for cancer therapy.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteogenic function of BMP2-modified PEEK scaffolds for orbital fracture repair.","authors":"Yujie Wu, Cuihong Liu, Jinhua Liu, Wenwen Wang, Bixuan Qin, Honglei Liu","doi":"10.1088/1748-605X/adc220","DOIUrl":"10.1088/1748-605X/adc220","url":null,"abstract":"<p><p>This study aimed to investigate the osteogenic function of polyetheretherketone (PEEK) scaffolds modified with bone morphogenetic protein 2 (BMP2) and its possibility for orbital fracture repair. The 3D-printed PEEK sheets were combined with BMP2-loaded hyaluronic acid hydrogel (HAH) to fabricate PEEK-BMP2-HAH composite scaffolds. Bone marrow mesenchymal stem cells (BMSCs) were seeded onto PEEK or PEEK-BMP2-HAH scaffolds. Cell adhesion and cell proliferation were measured by transmission electron microscopy and CCK-8 assay. Alkaline phosphatase (ALP) chromogenic, alizarine red S staining, and PCR analysis of Runt-related transcription factor 2 (Runx2), collagen-I (Col-I), Osterix, and osteopontin (OPN) were performed to assess osteogenic activity. The rat orbital fracture defect model is proposed for evaluating the biocompatibility, osteogenic integration, and functional recovery of PEEK orbital implants. Compared with PEEK, cell adhesion and cell proliferation were increased in PEEK-BMP2-HAH scaffolds. ALP activity and mineralized nodule formation were increased in PEEK-BMP2-HAH scaffolds than that in PEEK the mRNA expression of Runx2, Osterix, Col-I and OPN was increased on PEEK-BMP2-HAH scaffolds than that on PEEK at 14 d of osteogenic induction. Besides, a bone defect animal model revealed that BMP2-HAH-modified PEEK scaffolds could effectively facilitate the repair of the orbital bone defect, with increased expression of OPN and Runx2. BMP2-loaded HAH effectively increased adhesion, proliferation, and osteogenic differentiation of BMSCs on PEEK. PEEK-BMP2-HAH scaffolds are expected to become new materials for orbital fracture repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible nanozyme with dual catalytic activities for high-performance multimodality therapy against glioblastoma.","authors":"Guihong Lu, Xiaoyan Li, Wenfei Xu, Fan Zhang, Xiang Chen, Huibin Wu, Haibing Dai, Feng Li, Weidong Nie","doi":"10.1088/1748-605X/adc05b","DOIUrl":"10.1088/1748-605X/adc05b","url":null,"abstract":"<p><p>Nanozymes based on metals have been regarded as a promising candidate in the metabolic reprogramming of low-survival, refractory glioblastoma multiforme (GBM). However, due to size limitations, nanozymes struggle to balance catalytic activity with the ability to cross the blood-brain barrier (BBB), limiting their efficiency in GBM therapy. Herein, we establish a hybrid nanocluster, AuMn NCs, by cross-linking ultrasmall nano-gold (Au) and manganese oxide (MnO₂), which overcomes the size requirement conflict for integrating catalytic activities, long-period circulation, photothermal effect, glucose consumption, and chemodynamic effect for multimodality treatment against GBM. After administered intravenously, the overall large-size AuMn NCs can escape kidney filtration and cross the BBB for GBM accumulation. Then the individual ultrasmall nano-MnO₂components effectively catalyze H₂O₂degradation as catalase to produce oxygen, which is utilized by individual ultrasmall nano-Au components to consume glucose as glucose oxidase for starvation therapy. The H₂O₂generated during Au-catalyzed glucose consumption further facilitates MnO₂catalytic activity. Such positive feedback overwhelmingly intervenes in the glucose metabolism of GBM. Concurrently, clustered Au-induced photothermal effect and released Mn²⁺-induced chemodynamic effect further contribute to eliminating GBM cells. The versatile clustered nanozyme offers a feasible strategy for the multimodality intervention of GBM.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing visible light-induced 3D bioprinting: alternating extruded support materials for bioink gelation.","authors":"Takashi Kotani, Takehito Hananouchi, Shinji Sakai","doi":"10.1088/1748-605X/adc0d6","DOIUrl":"10.1088/1748-605X/adc0d6","url":null,"abstract":"<p><p>In 3D bioprinting, two promising approaches have gained significant attention: the use of support materials during printing and the utilization of bioinks gelled through ruthenium(II) tris-bipyridyl dication ([Ru(bpy)₃]²⁺)-catalyzed photocrosslinking consuming sodium persulfate (SPS). Integrating these approaches while ensuring simplicity and printability remains a challenge. To address this challenge, we propose a technique in which the support material containing SPS is alternately extruded with the bioink containing polymer having phenolic hydroxyl moieties (polymer-Ph) and [Ru(bpy)₃]²⁺under visible light irradiation. This method eliminates the problems of light shading and deformation caused by the support material, as the contact between the alternately extruded ink and the support material initiates the gelation of the ink via photocrosslinking. Using an ink containing 0.5 w/v% hyaluronic acid with phenolic hydroxyl moieties (HA-Ph) and 2.0 mM [Ru(bpy)₃]²⁺alongside a support material containing 10 mM SPS, various constructs were successfully printed under 450 nm visible light. The human hepatoblastoma cells embedded in the printed construct showed approximately 95% viability after printing and proliferation over 14 d of culture. These results highlight the potential of this method to advance 3D bioprinting for tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compression and bending performance of selective laser melted Ti6Al4V porous structures with cylindrical thin walls for dental implants.","authors":"Zefang Guo, Tianmin Guan, Mingli Liu, David Hui, Yun Zhai","doi":"10.1088/1748-605X/adc0d5","DOIUrl":"10.1088/1748-605X/adc0d5","url":null,"abstract":"<p><p>Titanium alloy dental implants play a crucial role in the field of oral rehabilitation. However, the use of solid designs can give rise to mechanical problems such as mismatched compressive elastic modulus with the host bone tissue, resulting in stress shielding and stress concentration. These problems have been a persistent bottleneck in their application effectiveness. To overcome this challenge, this study creatively designed five types of porous structures with cylindrical thin wall based on the Gibson-Ashby theoretical model. The aim is to optimize the mechanical performance of dental implants, enhance their compatibility with the host bone tissue, and utilize selective laser melting technology for precise fabrication of porous structures using Ti6Al4V material. Through a combination of simulation analysis and compression experiments, the stress and strain distributions of the five structures are systematically investigated under different bite conditions. The experimental results demonstrate that all five porous structures designed in this study effectively alleviate stress shielding phenomenon in dental implants, significantly improving the bonding performance between the implants and bone tissue. This meets the clinical implantation requirements and provides strong theoretical support for the application of dental implants in clinical settings.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic interactions between physical exercise intervention, innovative materials, and neurovascular coupling in bone repair and injury recovery: a comprehensive review.","authors":"Jiejie Guo, Ting Zhang, Mengjia Li, Qinwen Wang, Xianting Ding","doi":"10.1088/1748-605X/adc5c0","DOIUrl":"https://doi.org/10.1088/1748-605X/adc5c0","url":null,"abstract":"<p><p>Bone injury presents a prevalent challenge in clinical settings, with traditional treatment modalities exhibiting inherent limitations. Recent advancements have highlighted the potential of combining physical exercise intervention and innovative materials to enhance bone repair and recovery. This review explores the synergistic effects of physical exercise and novel materials in promoting bone regeneration, with a particular focus on the role of neurovascular coupling mechanisms. Physical exercise not only stimulates bone cell function and blood circulation but also enhances the bioactivity of novel materials, such as nanofiber membranes and smart materials, which provide supportive scaffolds for bone cell attachment, proliferation, and differentiation. Neurovascular coupling, involving the interaction between neural activity and blood flow, is integral to the bone repair process, ensuring the supply of nutrients and oxygen to the injured site. Studies demonstrate that the combination of physical exercise and novel materials can accelerate bone tissue regeneration, with exercise potentially enhancing the bioactivity of materials and materials improving the effectiveness of exercise. However, challenges remain in clinical applications, including patient variability, material biocompatibility, and long-term stability. Optimizing the integration of physical exercise and novel materials for optimal therapeutic outcomes is a key focus for future research. This review examines the collaborative mechanisms between physical exercise, novel materials, and neurovascular coupling, emphasizing their potential and the ongoing challenges in clinical settings. Further exploration is needed to refine their application and improve bone repair strategies.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}