Biomedical materials (Bristol, England)最新文献

{"title":"Loading curcumin on TiO₂nanotubes to improve surface biological activity.","authors":"Hanyu Peng, Jun Tan, Xiao Li","doi":"10.1088/1748-605X/ade488","DOIUrl":"10.1088/1748-605X/ade488","url":null,"abstract":"<p><p>Curcumin is a natural polyphenolic compound derived from turmeric, which exhibits a wide range of pharmacological activities, including anti-inflammatory and promoting bone healing effects. To enhance the bioactivity of the surface of titanium implants and promote early bone integration, the pure titanium surface was modified by composite modification through electrochemical anodic oxidation and drug coating. The surface of the prepared materials was characterized by scanning electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, and surface contact angle analyzer. The drug release performance of the modified titanium surfaces was evaluated by ultraviolet spectrophotometry. Rat bone marrow mesenchymal stem cells (BMSCs) were extracted and identified. The effects of surface modification on cell viability were investigated through CCK-8, cell adhesion, and live/dead cell staining experiments. The effects of different surface-treated titanium sheets on osteogenic differentiation of BMSCs were evaluated by transwell assay, alkaline phosphatase activity assay, reverse transcription quantitative polymerase chain reaction, and mineralization nodule staining experiments. The results showed that successful loading of TiO₂nanotubes with curcumin was prepared, and the surface-modified titanium sheets had effective physical properties (excellent corrosion resistance, mechanical properties and hydrophilicity) and drug release capabilities. The results of<i>in vitro</i>cell culture experiments indicated that superior cell adhesion morphology was observed on the surface of each group of titanium sheets. TiO₂nanotubes and curcumin could significantly promote BMSCs proliferation and showed pleasant biocompatibility. The<i>in vitro</i>osteogenic induction differentiation experiments confirmed that the TiO₂nanotube structure and curcumin coating could promote osteogenic differentiation of BMSCs. This study provides a significant theoretical foundation and experimental support for the development of bioactive implants for dental applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spinal fusion properties of mechanically-reinforced, osteomodulatory chitosan hydrogels.","authors":"Blake T Darkow, August J Hemmerla, Joseph P Herbert, Abigail R Grisolano, Austin D Kimes, John T Wray, Mark J Messler, Julien A Lanza, Yisheng Sun, Julia R Crim, J Derek Stensby, Ryan E Schultz, Lester J Layfield, Caixia Wan, Don K Moore, Bret D Ulery","doi":"10.1088/1748-605X/ade6b8","DOIUrl":"10.1088/1748-605X/ade6b8","url":null,"abstract":"<p><p>Lower back pain is a considerable medical problem that will impact 80% of the U.S. population at some point in their lifetime. For the most severe cases, surgical repair is necessary and is associated with annual costs upwards of $10 billion in the United States alone. To alleviate back pain, spinal fusions are a common treatment in which two or more vertebrae are biologically fused together often facilitated by a graft material. Unfortunately, iliac crest bone autograft, the current gold standard graft material, can yield insufficient fusion and is associated with considerable donor site morbidity and pain as well as is in limited supply. Therefore, new materials need to be developed in order to better coordinate healing and new bone growth in the affected area to reduce unnecessary patient burden. To address this issue, we incorporated allograft (AG) and cellulose (i.e⁰CNCs and CNFs) into a dual-crosslinked chitosan hydrogel loaded with bioactive calcium phosphate was investigated. Hydrogels were then tested for both their material and biological properties. Specifically, hydrogel swelling ratio, mass loss, ion release profile, compressive strength,<i>in vitro</i>biocompatibility and osteoinduction, and<i>in vivo</i>biocompatibility and effectiveness in a spine fusion model were assessed. Cellulose and AG incorporation significantly improved hydrogel compressive strength and biocompatibility and CNFs were found to be a significantly more biocompatible form of cellulose than⁰CNCs. Additionally, through the controlled delivery of osteoinductive simple signaling molecules (i.e. calcium and phosphate ions), dibasic calcium phosphate (DCF)-loaded CNF/chitosan hydrogels were able to induce osteoblast-like activity in murine mesenchymal stem cells. When evaluated<i>in vivo</i>, these hydrogels were found to be non-toxic through the subacute phase (i.e. up to 14 d). A 6 week rabbit spine fusion study found these materials excitingly achieved near complete fusion when assessed radiographically. This research provides considerable support for the utility of our novel complex biomaterial for spine fusion procedures as well as potentially for other future bone applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-intensity pulsed ultrasound as a strategy to boost exosome secretion by adipose-derived stem cells and uptake for Myocardial Infarction Therapy.","authors":"Riyue Jiang, Hao Wang, Fanglu Zhong, Yugang Hu, Junbi Liu, Yueying Chen, Wendi Su, Sheng Cao, Qing Deng, Qing Zhou","doi":"10.1088/1748-605X/ade925","DOIUrl":"https://doi.org/10.1088/1748-605X/ade925","url":null,"abstract":"<p><p>Acute myocardial infarction (AMI) remains a major global health issue, with limited therapeutic strategies to repair damaged myocardial tissue and improve long-term cardiac function. Exosome-based therapies, particularly those derived from adipose-derived stem cells (ADSCs), have shown significant promise in promoting cardiac repair. However, the low yield of exosomes from ADSCs has limited their clinical application. In this study, we investigate the potential of low-intensity pulsed ultrasound (LIPUS) to enhance exosome release from ADSCs and promote myocardial recovery. Our results demonstrate that LIPUS at 0.8 W/cm² for 10 minutes effectively increases ADSC-derived exosome production by approximately 50% through inhibiting autophagy. Additionally, LIPUS treatment promotes the uptake of exosomes by hypoxic myocardial cells, further enhancing the therapeutic potential of ADSC-exosomes in myocardial infarction. In vivo, the combination of LIPUS and exosomes significantly improved cardiac function, reduced inflammation, and attenuated myocardial apoptosis and fibrosis in a rat model of MI. These findings suggest that LIPUS can serve as a non-invasive strategy to boost exosome secretion and uptake, offering a promising approach for myocardial infarction therapy.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144512879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxiliary Electrode Tunes Wet-Electrospun Bundle Stiffness to Modulate Cell Phenotype.","authors":"Haoyu Wang, Chelsea Violita Stanley, Xiangshen Gao, Ziyu Liu, Mo Zhou, Mingjing Zhang, Fenglei Zhou, Maryam Tamaddom, Chaozong Liu","doi":"10.1088/1748-605X/ade8c6","DOIUrl":"https://doi.org/10.1088/1748-605X/ade8c6","url":null,"abstract":"<p><p>The stiffness of tissue-engineered scaffolds significantly influences cell behaviour and phenotype. However, current approaches to tuning stiffness often introduce unintended variations and compromise topographical consistency. In this study, an innovative wet-electrospinning set-up, incorporating a positively charged auxiliary electrode was developed to fabricate bundles with adjustable stiffness. COMSOL-based electromechanical computing revealed that the auxiliary electrode provided electrostatic force, which reduced stress concentration during continuous polycaprolactone (PCL) bundle collection at speeds up to 120 m/min. Tensile testing showed that increasing collection speed significantly enhanced bundle stiffness, with Young's modulus rising from 40 to 107 MPa. X-ray diffraction analysis indicated that this strengthen effect was associated with crystal disintegration and grain refinement within PCL fibre. These changes were reflected in scaffold stiffness, thereby, further influenced cell behaviour, as bundles with higher stiffness promoted a transition from non-polarized to spindle-like cell morphology. This electrostatic-assisted collection wet-electrospun setup enables the fabrication of scaffolds with tuneable mechanical properties while preserving topographical consistency, offering a robust strategy for mechanobiology research and tissue engineering.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-tuning equilibrium water content and mechanical properties of acrylic-based copolymers for intraocular lens applications.","authors":"Deniz Aki, Monireh Esmaeili Rad, Esat Can Şenel, Mesut Celil Onceyiz, Melih Can Gokmenoglu, Liviu Duta, Oguzhan Gunduz","doi":"10.1088/1748-605X/ade8c7","DOIUrl":"https://doi.org/10.1088/1748-605X/ade8c7","url":null,"abstract":"<p><p>We report on the development of copolymers of 2-Hydroxy Ethyl Acrylate (HEA) with 2-(2-Ethoxy Ethoxy) Ethyl Acrylate (EEEA) grafted with Ethylene Glycol Di-Methacrylate (EGDMA), designed for use as an intraocular lens (IOL) material. Various HEA/EEEA monomer ratios were synthesized via thermal copolymerization, with the HEA concentration pregressively increasing from 3.5% to 28%, while the EEEA concentration decreased proportionately. The physical-chemical, optical, and mechanical properties of the newly developed materials, fabricated as discs (~3.2 mm thick, 11 mm in diameter) and strips (~3.2 mm thick, 80×15 mm2), were comprehensively analyzed. Fourier-Transform Infrared Spectroscopy confirmed the successful copolymerization, as characteristic peaks corresponding to the monomers were observed. Since the development of IOL materials hinges on understanding their physical-chemical, optical, and mechanical characteristics - particularly the equilibrium water content (EWC) - our initial focus was on identifying EWC as a key factor in the development of IOLs. The results showed that the EWC value increased with higher HEA concentrations. Contact angle measurements indicated that the polymers exhibited hydrophilic behavior, with values ranging from 68 to 76°. X-ray diffraction analysis demonstrated that the HEA concentration influenced the crystalline structure, which, in turn, affected the mechanical properties. The results indicated that higher HEA concentrations, corresponding to increased EWC values (i.e., ~8%), led to enhanced flexibility, as evidenced by a decrease in tensile strength from 1.71 to 1.13 MPa, and reduced hardness, which declined from 57.5 to 47.5 Shore A. Additionally, refractive index analyses revealed a gradual decrease with increasing HEA concentrations, ranging from 1.565 to 1.543 when measured at 480 nm and from 1.547 to 1.528 when measured at 660 nm. The evaluation of the coefficient of variation and Pearson's correlation coefficient demonstrated strong material consistency and clear trends across formulations, reinforcing the reliability of the observed properties. These findings emphasize the significance of EWC and the ratio of hydrophilic monomers in acrylic-based copolymers, suggesting that future research could benefit from designing copolymers with tailored physical-chemical, optical, and mechanical properties for IOL applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-design material as scaffold module proposed to surgical application in osteoarthritis: fabrication, characterization, biological evaluation of in vitro testing.","authors":"Atsadaporn Thangprasert, Anne Bernhardt, Elke Gossla, David Kilian, Saranyoo Klaiklay, Jirut Meesane, Papitchaya Srithep, Michael Gelinsky, Pakorn Pasitsuparoad","doi":"10.1088/1748-605X/ade7e4","DOIUrl":"https://doi.org/10.1088/1748-605X/ade7e4","url":null,"abstract":"<p><p>Treatment of osteochondral defects is a major topic of current research and becomes more important in an aging society. The challenges in bone and cartilage repair arise from structure and function of these different tissues. This study proposes a biphasic model combining cartilage and bone scaffolds based on silk fibroin (SF) biopolymers. For cartilage phase, SF scaffolds were coated with gelatin and/or agarose layers. For bone scaffolds, mineralized collagen solutions were coated on or mixed into the SF matrix. The physical and biological properties of these samples were evaluated to find the optimum conditions for a biphasic scaffold. Modification of both cartilage and bone scaffolds resulted in smaller pore size, lower swelling rate and higher rigidity. Gelatin significantly promoted cartilage biomarker production and agarose facilitated cell proliferation, inducing a homogenous cell distribution, and stimulating chondrogenesis. Furthermore, modification with mineralized collagen decreased cell proliferation in osteoblast progenitors but enhanced differentiation into osteoblasts. The optimum conditions were found at a mixture of gelatin and agarose for coating in case of cartilage phase and low mineralized collagen content for bone phase.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome as a stable carrier for anti-inflammatory phenylpropanoid metabolites: a proof-of-concept study.","authors":"Shirali Patel, Neeraja Revi, Suridh Chakravarty, Aleksandra Gurgul, Yahya Najjar, Chun-Tao Che, Katherine Mary Warpeha, Divya Bijukumar","doi":"10.1088/1748-605X/ade7e3","DOIUrl":"https://doi.org/10.1088/1748-605X/ade7e3","url":null,"abstract":"<p><p>Phenylpropanoids (PA), which are plentiful in cruciferous vegetables, have not received adequate attention for their anti-inflammatory properties. Despite their potential benefits, the bioavailability and stability of these and other natural compounds under physiological conditions remain limited. This study aims to develop a natural nanovesicular delivery system that efficiently incorporates a phenylpropanoid extract-specifically, a multi-component anti-inflammatory extract derived from broccoli-with the goal of enhancing its bioavailability. This initiative serves as proof of concept for further research and application. The findings suggest that phenylpropanoids (PAs) achieve a 75% encapsulation efficiency within exosomes. Furthermore, it has been observed that PAs encapsulated in exosomes demonstrate a stability that is twice that of unencapsulated PAs under physiological conditions. The encapsulation process also improved the cytocompatibility of the PAs. Moreover, the functionality of the encapsulated PAs is significantly improved, as evidenced by a fivefold reduction in nitric oxide production from the EXO/PA nanocarriers. There is a significant decrease in the expression of pro-inflammatory genes, such as NFkB, MMP2, COX-2, and IL-1β, in comparison to cells treated with LPS. Moreover, levels of TNF-α, IL-6, and MCP-1 in activated macrophages treated with EXO/PAs were observed to be significantly reduced compared to those activated by LPS. It appears that the immune-suppressive effect of the extract may be mediated through both the ERK/MAPK and IkB/NFkB signaling pathways, highlighting the potential benefits of this approach. In conclusion, the results demonstrate that exosomes can effectively deliver polyphenylpropanoids while improving their stability and functionality, underscoring their potential role in future medical treatments.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aloe vera-enriched collagen-polyurethane hydrogel: Supporting tissue regeneration, antibacterial action and drug release for effective wound healing.","authors":"Maria I León-Campos, Jesús A Claudio-Rizo, Juan J Becerra-Rodriguez, Laura Espindola-Serna, Lucía F Cano-Salazar, Nayeli Rodríguez-Fuentes, Rebeca Betancourt-Galindo","doi":"10.1088/1748-605X/ade7e2","DOIUrl":"https://doi.org/10.1088/1748-605X/ade7e2","url":null,"abstract":"<p><p>Aloe vera is widely recognized for its healing, anti-inflammatory, moisturizing, and antibacterial properties, which are attributed to its rich biochemical composition, including polysaccharides, proteins, vitamins, and anthraquinones such as aloin and emodin. This study investigates the encapsulation of Aloe vera in collagen-polyurethane hydrogels to create a novel bioactive formulation for wound healing. Hydrogels with Aloe vera content ranging from 20-60 wt.% were prepared, forming scaffolds with a fibrillar-granular morphology and semi-crystalline surface. Encapsulation of Aloe vera resulted in semi-interpenetrating polymer networks (semi-IPNs), with the semi-interpenetration rate increasing as Aloe vera content rose. The formulation containing 60 wt.% Aloe vera significantly enhanced the superabsorbent capacity (2850 ± 210 %) and crosslinking degree (38 ± 3 %) of the matrix while reducing the loss factor (2.5 ± 0.4), resulting in a highly viscous gel ideal for moisture retention and uniform wound coverage. These hydrogels exhibited resistance to biodegradation in the presence of pepsin at skin pH (5.5) and demonstrated notable antibacterial activity, inhibiting E. coli (78 %) and S. aureus (57 %) growth compared to 40 ppm gentamicin. Additionally, they facilitated a gradual release of ketorolac (up to 65 % at pH 5.5 with 20 wt.% Aloe vera). The hydrogels were non-hemolytic and promoted the metabolic activity of monocytes and fibroblasts, enhancing cellular growth. Furthermore, the composition induced no inflammatory response but significantly boosted the secretion of the anti-inflammatory cytokine TGF-β1, potentially enhancing wound healing.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyapatite-induced bioactive and cell-imprinted polydimethylsiloxane surface to accelerate osteoblast proliferation and differentiation: an in vitro study on preparation and differentiating capacity.","authors":"Morteza Mehrjoo, Akbar Karkhaneh, Masoumeh Haghbin Nazarpak, Mostafa Alishahi, Shahin Bonakdar","doi":"10.1088/1748-605X/ade5e0","DOIUrl":"https://doi.org/10.1088/1748-605X/ade5e0","url":null,"abstract":"<p><p>Healing bone defects remains a significant orthopedic challenge. Cell therapy and tissue engineering offer promising solutions; however, obtaining high-quality, partially or fully differentiated cells remains difficult. Therefore, developing suitable substrates to guide stem cell differentiation helps in achieving this goal. Here, an optimized polydimethylsiloxane (PDMS) substrate was created by casting the PDMS composition on isolated and fixed human osteoblasts and characterizing the biological and surface features of cell patterns. A nanolayer of hydroxyapatite (nHA) was sputtered on the cell patterns to mimic the bone extracellular matrix and enhance osteo- differentiation, providing both physical and chemical stimulations. Various physical and biological properties of patterned and non-patterned PDMS substrates with and without nHA coating were evaluated to confirm the osteo-differentiation of adipose derived mesenchymal stem cells capacity. According to the results, precise cell imprinting was successfully achieved, and nHA deposition did not adversely affect the surface topography. All substrates were biocompatible, and the combination of physical (cell imprinting)-chemical (nHA coating) stimuli significantly enhanced stem cell differentiation, as evidenced by increased alkaline phosphatase activity, upregulation of bone-specific genes, and calcium deposition. A well-designed PDMS substrate can be promising for providing osteo-differentiated stem cells in large quantities for various cell therapy and tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and in vivo characterization of FRESH-based 3D printed chitosan construct for small intestine regeneration.","authors":"Parul Chaurasia, Richa Singh, Rishabh Rai Kaushik, Narayan Yadav, Sanjeev Kumar Mahto","doi":"10.1088/1748-605X/ade5e1","DOIUrl":"https://doi.org/10.1088/1748-605X/ade5e1","url":null,"abstract":"<p><p>This study demonstrates the implantation of a 3D-printed small intestine construct using chitosan bioink and freeform reversible embedding of suspended hydrogels (FRESH) bioprinting technology. The research addresses the significant clinical challenges posed by inflammatory bowel disease (IBD) and short bowel syndrome (SBS), which often require surgical interventions leading to substantial loss of small intestine (SI) surface area. High costs, side effects, and donor shortages limit traditional treatments such as total parenteral nutrition and small bowel transplantation. Therefore, developing an engineered artificial intestine represents a critical need. The study employed a natural biopolymer, i.e., chitosan, known for its biocompatibility and blood compatibility, as the primary material for the bioink. The 3D-bioprinted constructs were evaluated through mechanical characterization, blood biocompatibility tests, and antibacterial assays. The mechanical properties indicated the constructs' ability to withstand significant deformation, while the blood compatibility tests showed minimal hemolysis, supporting the material's suitability for implantation. Antibacterial tests revealed that the constructs could inhibit bacterial growth, reducing the risk of implant-associated infections. Following the implantation of the prepared constructs in rats, the post-implantation analysis indicated successful integration and biocompatibility with no significant adverse reactions. The biochemical parameters, like inflammatory markers, were found to be slightly higher than the normal range. All other parameters, like bilirubin and albumins, etc, were in the normal range. This study highlights the potential of 3D-bioprinted chitosan-based constructs in organ regeneration and presents a promising solution for treating SBS and IBD. The findings support further exploration of the fabricated 3D printed biocompatible materials for medical applications in regenerative medicine and tissue engineering.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}