Biomedical materials (Bristol, England)最新文献

{"title":"Hydroxyapatite-induced bioactive and cell-imprinted polydimethylsiloxane surface to accelerate osteoblast proliferation and differentiation: an in vitro study on preparation and differentiating capacity.","authors":"Morteza Mehrjoo, Akbar Karkhaneh, Masoumeh Haghbin Nazarpak, Mostafa Alishahi, Shahin Bonakdar","doi":"10.1088/1748-605X/ade5e0","DOIUrl":"https://doi.org/10.1088/1748-605X/ade5e0","url":null,"abstract":"<p><p>Healing bone defects remains a significant orthopedic challenge. Cell therapy and tissue engineering offer promising solutions; however, obtaining high-quality, partially or fully differentiated cells remains difficult. Therefore, developing suitable substrates to guide stem cell differentiation helps in achieving this goal. Here, an optimized polydimethylsiloxane (PDMS) substrate was created by casting the PDMS composition on isolated and fixed human osteoblasts and characterizing the biological and surface features of cell patterns. A nanolayer of hydroxyapatite (nHA) was sputtered on the cell patterns to mimic the bone extracellular matrix and enhance osteo- differentiation, providing both physical and chemical stimulations. Various physical and biological properties of patterned and non-patterned PDMS substrates with and without nHA coating were evaluated to confirm the osteo-differentiation of adipose derived mesenchymal stem cells capacity. According to the results, precise cell imprinting was successfully achieved, and nHA deposition did not adversely affect the surface topography. All substrates were biocompatible, and the combination of physical (cell imprinting)-chemical (nHA coating) stimuli significantly enhanced stem cell differentiation, as evidenced by increased alkaline phosphatase activity, upregulation of bone-specific genes, and calcium deposition. A well-designed PDMS substrate can be promising for providing osteo-differentiated stem cells in large quantities for various cell therapy and tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and in vivo characterization of FRESH-based 3D printed chitosan construct for small intestine regeneration.","authors":"Parul Chaurasia, Richa Singh, Rishabh Rai Kaushik, Narayan Yadav, Sanjeev Kumar Mahto","doi":"10.1088/1748-605X/ade5e1","DOIUrl":"https://doi.org/10.1088/1748-605X/ade5e1","url":null,"abstract":"<p><p>This study demonstrates the implantation of a 3D-printed small intestine construct using chitosan bioink and freeform reversible embedding of suspended hydrogels (FRESH) bioprinting technology. The research addresses the significant clinical challenges posed by inflammatory bowel disease (IBD) and short bowel syndrome (SBS), which often require surgical interventions leading to substantial loss of small intestine (SI) surface area. High costs, side effects, and donor shortages limit traditional treatments such as total parenteral nutrition and small bowel transplantation. Therefore, developing an engineered artificial intestine represents a critical need. The study employed a natural biopolymer, i.e., chitosan, known for its biocompatibility and blood compatibility, as the primary material for the bioink. The 3D-bioprinted constructs were evaluated through mechanical characterization, blood biocompatibility tests, and antibacterial assays. The mechanical properties indicated the constructs' ability to withstand significant deformation, while the blood compatibility tests showed minimal hemolysis, supporting the material's suitability for implantation. Antibacterial tests revealed that the constructs could inhibit bacterial growth, reducing the risk of implant-associated infections. Following the implantation of the prepared constructs in rats, the post-implantation analysis indicated successful integration and biocompatibility with no significant adverse reactions. The biochemical parameters, like inflammatory markers, were found to be slightly higher than the normal range. All other parameters, like bilirubin and albumins, etc, were in the normal range. This study highlights the potential of 3D-bioprinted chitosan-based constructs in organ regeneration and presents a promising solution for treating SBS and IBD. The findings support further exploration of the fabricated 3D printed biocompatible materials for medical applications in regenerative medicine and tissue engineering.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Nanomedicine for Cancer Theranostics.","authors":"Sudip Mukherjee, Aravind Kumar Rengan, Chitta Ranjan Patra","doi":"10.1088/1748-605X/addf26","DOIUrl":"https://doi.org/10.1088/1748-605X/addf26","url":null,"abstract":"<p><p>Cancer is one of the foremost reasons for global death. According to estimates, around 19.3 million instances of cancer and over 10 million fatalities were documented in the year 2020, making it one of the leading causes of death across the globe. There are still restrictions due to the absence of effective early detection and inadequate conventional therapy, which has led to poor prognosis and survival rates. This is the case despite the fact that there have been breakthroughs in diagnosis and treatment. The science of nanomedicine has achieved considerable advancements in the realm of cancer theranostics. These advancements offer a number of distinct advantages, including tumor-targeting through the increased permeability and retention effect, biocompatibility, and small size. In light of the above, the purpose of the Focus Issue on 'Advances in Nanomedicine for Cancer Theranostics', was introduced to highlight new research on nanomedicine-based approaches to cancer treatment. These techniques include drug and gene delivery, bioimaging, biomarker identification, diagnosis, immunotherapy, biosensors, and other precision oncology strategies. For this Focus Issue, we invited front-line researchers and authors who contributed the original research papers and topical review articles. This editorial summarizes the published articles of this collection which includes eighteen research articles and eleven review articles.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed polymeric biomaterials in bone tissue engineering.","authors":"Tianyi Xia, Xianglong Zhou, Haoran Zhou, Jiheng Xiao, Jianhui Xiang, Hanhong Fang, Liming Xiong, Fan Ding","doi":"10.1088/1748-605X/ade18d","DOIUrl":"10.1088/1748-605X/ade18d","url":null,"abstract":"<p><p>Polymers are large molecules composed of repeating subunits called monomers, which can be de-rived from both natural sources and synthetic processes. Due to their exceptional physicochemical properties and functional characteristics, polymers have garnered significant attention in the bio-medical field, particularly in tissue engineering. 3D printing technology, a process that manufactures three-dimensional objects by sequentially adding material based on digital models, has been widely recognized for its integration with polymers in bone tissue engineering (BTE). This review provides an overview of 3D-printed polymeric biomaterials in BTE. It begins with a discussion of the fundamental process of bone regeneration, followed by a component's selection for polymers and 3D printing technologies. Additionally, this review comprehensively addresses the functional properties design of 3D-printed polymeric biomaterials. Finally, the current status, challenges, and future directions for the application of 3D-printed polymeric biomaterials in BTE are discussed.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed β-TCP/curcumin scaffolds as a local drug delivery system for bone tissue regeneration.","authors":"Lilian de Siqueira, Marcela Arango Ospina, Dayane Batista Tada, Dachamir Hotza, Eliandra de Sousa Trichês, Aldo R Boccaccini","doi":"10.1088/1748-605X/ade109","DOIUrl":"10.1088/1748-605X/ade109","url":null,"abstract":"<p><p>The growing clinical need for filling defects and bone voids has led to the development of scaffolds that stimulate bone regeneration and serve as temporary models for vascularised bone growth. Additionally, these scaffolds can function as drug delivery systems to reduce inflammatory processes associated with diseases such as osteoarthritis, rheumatoid arthritis, osteoporosis, and bone cancer. Among the materials used to manufacture scaffolds,<i>β</i>-tricalcium phosphate (<i>β</i>-TCP, Ca₃(PO₄)₂) stands out due to its excellent biocompatibility and chemical composition, closely resembling minerals from bone tissue. When combined with curcumin, calcium phosphate scaffolds offer a promising platform for drug delivery, as their tailored porous structure can provide controlled release. Curcumin enhances anti-inflammatory and antioxidant properties, thereby promoting tissue regeneration. In this study,<i>β</i>-TCP powders loaded with 5 and 10 mg ml^-1of curcumin (designated as<i>β</i>-TCP/Curc 5 and<i>β</i>-TCP/Curc 10) were successfully obtained via freeze-drying and characterised using x-ray diffraction and Fourier-transform infrared spectroscopy to assess their crystallinity and chemical composition. The<i>β</i>-TCP/Curc powders were evaluated for their ability to load and release curcumin. Subsequently,<i>β</i>-TCP and<i>β</i>-TCP/Curc 5 scaffolds were prepared using 3D printing. The<i>β</i>-TCP/Curc 5 scaffolds were assessed for curcumin release, cytotoxicity profile, and antimicrobial activity. The<i>β</i>-TCP/Curc 5 powders exhibited significantly higher curcumin adsorption and good release capacity, whereas the<i>β</i>-TCP/Curc 10 powders displayed reduced curcumin loading and limited release efficiency. The combination of<i>β</i>-TCP/Curc 5 with sodium alginate produced a suitable paste for 3D printing scaffold fabrication, and the<i>β</i>-TCP/Curc 5 scaffolds demonstrated high similarity to the computational model. Importantly, the<i>β</i>-TCP scaffolds did not exhibit cytotoxicity in the MC3T3-E1 cell line, and after curcumin loading, there was no increase in cellular cytotoxicity observed. In fact, an increase in cell viability was noted compared to the control after three days of indirect assays, suggesting that this combination may be beneficial for promoting cell growth. However, the scaffolds did not show any antibacterial effects against<i>S. aureus</i>and<i>E. coli</i>under the tested conditions. This study demonstrates that adequate curcumin loading in 3D-printed<i>β</i>-TCP scaffolds can facilitate curcumin release at the bone healing site, potentially influencing the cellular processes involved in bone regeneration and remodelling.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights of right ventricular anisotropic hysteresis behavior with pulmonary hypertension development.","authors":"Kristen LeBar, Kellan Roth, Wenqiang Liu, Brandyn Garcia, Jassia Pang, Jessica Ayers, Adam J Chicco, Zhijie Wang","doi":"10.1088/1748-605X/ade108","DOIUrl":"10.1088/1748-605X/ade108","url":null,"abstract":"<p><p>There is growing evidence of myocardial hysteresis and, recently, the viscoelasticity of healthy and pulmonary hypertensive (PH) right ventricle free walls (RVFW) has been studied by stress-relaxation. However, stress-relaxation does not fully capture the<i>in vivo</i>deformation of the tissue, and the changes in right ventricle hysteresis behavior with PH remain unknown. Our aim was to investigate RVFW biaxial hysteresis behavior with PH. We conducted equibiaxial cyclic sinusoidal tensile testing in healthy and PH rat RVFW tissues under 20% strain, with strain rates of 0.1&1 Hz (sub-physiological), and 5&8 Hz (physiological). Elastic modulus, loop height, stored and dissipated energies, the ratio of viscosity to elasticity (V/E), and the percentage of dissipated to total energy (damping) were derived. After PH, elastic modulus was elevated in both directions, while longitudinal loop height and stored and dissipated energies were increased (<i>p</i>< 0.05). Despite these individual changes in viscosity and elasticity, V/E ratio and damping were maintained. We further found frequency-dependent responses of V/E ratio and damping, and these were enhanced in the diseased RVs (<i>p</i>< 0.05 at 5&8 Hz) than healthy RVs (<i>p</i>< 0.05 only at 8 Hz). Finally, we observed significant correlations between individual mechanical properties and structural changes (collagen content/myofiber width), and the correlations were stronger in the longitudinal (<i>p</i>⩽ 0.006) than circumferential (<i>p</i>< 0.05) direction. Moreover, collagen had a much greater contribution (<i>p</i>⩽ 0.002) to tissue elasticity than myofiber (<i>p</i>⩽ 0.02). Multiple linear regression analyses revealed a significant role of myofibers, not collagen content, in the tissue viscosity in both directions (<i>p</i>< 0.05). Our results suggest the importance of incorporating tissue viscoelastic properties into pathophysiology as well as the design of cardiac biomimetic materials for advancements in cardiac health.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loading curcumin on titanium nanotubes to improve surface biological activity.","authors":"Hanyu Peng, Jun Tan, Xiao Li","doi":"10.1088/1748-605X/ade488","DOIUrl":"https://doi.org/10.1088/1748-605X/ade488","url":null,"abstract":"<p><p>Curcumin is a natural polyphenolic compound derived from turmeric, which exhibits a wide range of pharmacological activities, including anti-inflammatory and promoting bone healing effects. To enhance the bioactivity of the surface of titanium implants and promote early bone integration, the pure titanium surface was modified by composite modification through electrochemical anodic oxidation and drug coating. The surface of the prepared materials was characterized by scanning electron microscopy , atomic force microscopy, X-ray photoelectron spectroscopy, and surface contact angle analyzer. The drug release performance of the modified titanium surfaces was evaluated by ultraviolet spectrophotometry. Rat bone marrow mesenchymal stem cells were extracted and identified. The effects of surface modification on cell viability were investigated through CCK-8, cell adhesion, and live/dead cell staining experiments. The effects of different surface-treated titanium sheets on osteogenic differentiation of bone marrow mesenchymal stem cells were evaluated by transwell assay, alkaline phosphatase activity assay, reverse transcription quantitative polymerase chain reaction , and mineralization nodule staining experiments. The results showed that successful loading of titanium nanotubes with curcumin was prepared, and the surface-modified titanium sheets had effective physical properties (excellent corrosion resistance, mechanical properties and hydrophilicity) and drug release capabilities. The results of in vitro cell culture experiments indicated that superior cell adhesion morphology was observed on the surface of each group of titanium sheets. TiO2 nanotubes and curcumin could significantly promote bone marrow mesenchymal stem cells proliferation and showed pleasant biocompatibility. The in vitro osteogenic induction differentiation experiments confirmed that the TiO2 nanotube structure and curcumin coating could promote osteogenic differentiation of bone marrow mesenchymal stem cells. This study provides a significant theoretical foundation and experimental support for the development of bioactive implants for dental applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In-vitro</i>evaluation of<i>in-situ</i>synthesized superparamagnetic iron nanoparticles (SPINs) for magnetic hyperthermia treatment of breast and prostate cancer.","authors":"Mariam Elabbasi, Ahmed A El-Gendy","doi":"10.1088/1748-605X/addf27","DOIUrl":"10.1088/1748-605X/addf27","url":null,"abstract":"<p><p>Magnetic hyperthermia has emerged as a promising approach in the pursuit of effective cancer therapies; however, its success relies heavily on the development of advanced magnetic nanomaterials. This study introduces a groundbreaking approach of intracellular magnetic hyperthermia using superparamagnetic iron nanoparticles (SPINs) specifically within breast and prostate tumors, laying a crucial foundation for the development of hyperthermia cancer therapy. In contrast to traditional anticancer treatments, our approach leverages the superior tumor retention capabilities of nanoparticles due to their intracellular cell uptake allowing efficient induced localized heating power. We developed a highly controllable synthesis method for iron nanoparticles in carbon matrix, which exhibit efficient localized heat generation by SPINs under applied magnetic field within the clinical limit, with magnetic saturation exceeding 150 emu g^-1, highlighting their potential for hyperthermia therapy. Characterization through scanning electron microscopy, x-ray diffraction, and vibrating sample magnetometry confirms the spherical-like shape, pure iron phase and high magnetization of the formed nanoparticles. These dispersed nanoparticles demonstrate feasibility for hyperthermia, quantified by the specific absorption rate.<i>In vitro</i>intracellular uptake studies using Du145 prostate and MCF7 breast cancer cell lines indicate efficient nanoparticle tumor cell-uptake. Pre- and post-hyperthermia cell viability assessments show substantial tumor cell death, with nearly 50% reduction post-magnetic field application. These findings highlight the promising potential of these advanced nanoparticles for intracellular targeted cancer therapy, particularly in solid tumors, and suggest significant avenues for further medical research and application.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing biomedical applications: integrating textile innovations with tissue engineering.","authors":"Joyjit Ghosh, Nishat Sarmin Rupanty, Tanvir Rahman Asif, Tasneem Noor, Tarikul Islam, Vladimir Reukov","doi":"10.1088/1748-605X/adda81","DOIUrl":"10.1088/1748-605X/adda81","url":null,"abstract":"<p><p>Textile technologies are significantly advancing the field of tissue engineering (TE) by providing innovative scaffolds that closely mimic the extracellular matrix and address crucial challenges in tissue regeneration. Techniques such as weaving, knitting, and braiding allow for creating structures with customizable porosity, mechanical properties, and fiber alignment, which are essential for supporting cellular behaviors such as adhesion, proliferation, and differentiation. Recent developments have incorporated bioactive materials-like growth factors, peptides, and nanoparticles-into these textile-based scaffolds, greatly enhancing their functionality for applications in wound healing, skin regeneration, and organ engineering. The emergence of smart textiles, which utilize responsive polymers and nanotechnology, facilitates the on-demand delivery of therapeutic agents and provides electrical stimulation to repair neural and muscular tissues. Additionally, combining 3D bioprinting with textile principles enables the fabrication of anatomically precise, multi-layered scaffolds, expediting advancements in complex tissue reconstruction, including vascular grafts and bone scaffolds. Utilization of materials such as polycaprolactone, collagen, and silk fibroin-often in hybrid forms-ensures that these scaffolds maintain biocompatibility, mechanical integrity, and biodegradability. As functionalized textiles are explored for applications in cardiovascular, skin, and organ engineering, leveraging techniques like electro-spun nanofibers and braided vascular grafts, a transformative approach to regenerative medicine emerges. Despite ongoing challenges with vascularization and scaling, textile-engineered scaffolds promise to enable personalized, durable, and multifunctional solutions, positioning the convergence of textile science and TE to redefine future biomedical applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144102002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fib@PEGDA/GelMA hydrogel as a light-curing thin-layer matrix for RPE cell growth and function.","authors":"Naiwen Zhang, Cong Ma, Fei Shao, Huanan Wang, Xiang Ma","doi":"10.1088/1748-605X/addcab","DOIUrl":"10.1088/1748-605X/addcab","url":null,"abstract":"<p><p>Retinal degenerative diseases, including age-related macular degeneration and retinitis pigmentosa, are leading causes of blindness globally, characterized by progressive degeneration of retinal pigment epithelium (RPE) and photoreceptor (PR) cells. Despite advancements, current therapies have not substantially arrested disease progression. Cell replacement therapy using healthy RPE and PR cells holds promise but faces obstacles such as poor cell survival, inadequate integration, and transplantation difficulties. To address these issues, tissue engineering combined with 3D printing has become a focal point. This study investigates the use of four hydrogels-GelMA, HAMA, AlgMA, and PEGDA-and their various crosslinked combinations for creating hydrogel thin-layer matrices conducive to RPE cell growth. PEGDA/GelMA hydrogel demonstrated optimal support for cell spreading and proliferation, which is not achievable with hydrogels matrices of other formulations. The relationship between the mechanical properties of PEGDA/GelMA hydrogels and cell growth was further refined. PEGDA600-20 hydrogel with a compressive modulus of 1245.07 ± 20.79 kPa was selected based on time-course viability assays, leading to the development of the optimized Fib@PEGDA/GelMA hydrogel exhibited exceptional biocompatibility. Compared to PEGDA/GelMA, CCK-8 assays demonstrated significantly improved relative cell viability at 24 h, 48 h, and 72 h, with increases of 17.73 ± 1.22%, 14.54 ± 3.63%, and 19.04 ± 2.31%, respectively on Fib@PEGDA/GelMA matrix. qRT-PCR results indicated a mitigation of epithelial-mesenchymal transition (EMT), as evidenced by downregulation of EMT markers (CDH2, COL1A1, and FN1), accompanied by reduced IL-6 levels. Fib@PEGDA/GelMA hydrogel enhanced phagocytic activity in ARPE-19 cells and promoted functional expression in hiPSC-RPEs. Additionally, the hydrogel showed favorable<i>in vivo</i>biocompatibility following subcutaneous implantation of RCS rats at 1, 2, and 4 weeks post-implantation evidenced by HE and Masson's staining. This system offers a promising bioink for 3D-printed retinal cell scaffolds and paves the way for future advancements in cell replacement therapies for retinal degenerative diseases.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}