Biomedical materials (Bristol, England)最新文献

{"title":"Particle stabilised high internal phase emulsion scaffolds with interconnected porosity facilitate cell migration.","authors":"Areli Munive-Olarte, Enes Durgut, Stefaan W Verbruggen, Frederik Claeyssens, Gwendolen C Reilly","doi":"10.1088/1748-605X/ae05de","DOIUrl":"10.1088/1748-605X/ae05de","url":null,"abstract":"<p><p>A key challenge in bone tissue engineering (BTE) is designing structurally supportive scaffolds, mimicking the native bone matrix, yet also highly porous to allow nutrient diffusion, cell infiltration, and proliferation. This study investigated the effect of scaffold interconnectivity on human bone marrow stromal cell (BMSC) behaviour. Highly interconnected, porous scaffolds (polyHIPEs) were fabricated using the emulsion templating method from 2-ethylhexyl acrylate/isobornyl acrylate (IBOA) and stabilised with ∼200 nm IBOA particles. Pore interconnectivity was tuned by varying the internal phase fraction from 75%-85% and characterised by the degree of openness, Euler number, frequency, and size of pore interconnects. The attachment, proliferation, infiltration, and osteogenic differentiation of the BMSC cell line (Y201) were evaluated on these scaffolds. Results showed that high pore interconnectivity facilitated diffusion and cell infiltration throughout the scaffolds. Furthermore, the most interconnected scaffolds enhanced osteogenic differentiation of Y201 cells, as evidenced by elevated alkaline phosphatase activity and increased calcium and collagen production compared to less interconnected scaffolds. These findings emphasise the importance of scaffold interconnectivity in BTE for efficient nutrient transport, facilitating cell migration and infiltration, and supporting the development of interconnected cell networks that positively influence osteogenic differentiation.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pH controlled release of extracellular vesicles from a hydrogel scaffold for therapeutic applications.","authors":"Simon Chewchuk, Nicholas Soucy, Fan Wan, James Harden, Michel Godin","doi":"10.1088/1748-605X/ae0777","DOIUrl":"10.1088/1748-605X/ae0777","url":null,"abstract":"<p><p>Cell-based therapies are gaining attention as a promising approach for repairing damaged tissues and organs, offering alternatives to invasive treatments like organ transplants and powerful medications. Recent research has shifted towards extracellular vesicles (EVs), membrane-bound particles that can carry therapeutic compounds like DNA, RNA, and proteins, which may offer advantages over cell-based therapies, such as higher potency and reduced immune reactions. A key challenge in EV therapy is ensuring that the vesicles reach their intended target tissues. While EVs are often delivered via injection, systemic administration can result in off-target effects. To address this, we highlight the microfluidic encapsulation of EVs in hydrogel microcapsules that include a CD9 binding peptide (CD9BP), allowing for controlled EV release in response to a shift in environmental pH. By encapsulating CD9+ EVs in CD9BP hydrogel capsules, we demonstrate the release of their contents in acidified environments typical of damaged tissues. This method allows for targeted, localized EV delivery. The approach promises more effective tissue regeneration while reducing the need for broad, non-specific drug delivery.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145076160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recellularization of scaffolds derived from precision-cut kidney slices.","authors":"Haitham Salti, Sophie-Charlotte Nelz, Sarina Lichtwark, Christopher Pohl, Lea Kramer, Mathias Lorenz, Heiko Lemcke, Sandra Doss, Steffen Mitzner, Reinhold Wasserkort","doi":"10.1088/1748-605X/ae05a4","DOIUrl":"10.1088/1748-605X/ae05a4","url":null,"abstract":"<p><p>The global rise in chronic kidney disease necessitates innovative solutions for end-stage renal disease that can help to overcome the limitations of the only available treatment options, transplantation and dialysis. Tissue engineering presents a promising alternative, leveraging decellularized scaffolds to retain the extracellular matrix (ECM). However, optimizing methods for decellularization and recellularization remains a challenge. Here we present novel work which builds on our previous study where we investigated several decellularization protocols. In this study we analyzed the suitability of decellularized scaffolds for recellularization. Precision-cut kidney slices (PCKS) were utilized as a model to explore the impact of different decellularization protocols on scaffold recellularization. PCKS were pretreated physically followed by immersion decellularization in chemicals (CHEM-Imm). Physical pretreatments included high hydrostatic pressure (HHP-Imm) or freezing-thawing cycles (FTC-Imm). Scaffolds were recellularized, with human renal proximal tubular epithelial cells (RPTEC/TERT1). All scaffolds showed cell growth over the 7 d incubation period. Notably, FTC-Imm demonstrated the highest expression of the tight junction protein zonula-occludens-1 (ZO-1). Moreover, as the native kidney is composed of up to 30 different cell types, we utilized artificial neural networks to investigate the distribution and attachment patterns of RPTEC/TERT1 cells to determine if decellularized scaffolds retain cell specific attachment sites. It was revealed that, at least 97% of RPTEC/TERT1 cells were attached outside the Bowman capsules, potentially showing a clear tendency to attach to their original tubular sites. This suggests that the ECM retains instructive cues guiding the migration and attachment of the cells. Overall, our scoring system identified FTC-Imm as the most effective method.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inorganic polyphosphate, a paradigm changer in 3D printing of<i>β</i>-tricalcium phosphate based materials for bone tissue surgery.","authors":"Meik Neufurth, David Molter, Xiaoqin La, Changxin Wu, Hiroshi Ushijima, Heinz C Schröder, Xiaohong Wang, Werner E G Müller","doi":"10.1088/1748-605X/ae084b","DOIUrl":"10.1088/1748-605X/ae084b","url":null,"abstract":"<p><p><i>β</i>-Tricalcium phosphate (<i>β</i>-TCP) is widely used as a material for bone implants due to its excellent biocompatibility, biodegradability, and osteoconductivity, as well as its osteoinductive properties. Here, we demonstrate that the regenerative potential of this material can be significantly enhanced when incorporated into a matrix of inorganic polyphosphate (polyP), a physiological, metabolically active polymer composed of phosphate residues linked by high-energy phosphoanhydride bonds. A 3D-printable hydrogel was developed containing suspended<i>β</i>-TCP and amorphous calcium-polyP nanoparticles (Ca-polyP-NP; the water-insoluble depot form of polyP), as well as NaH₂PO₄as the monomeric precursor of the polymeric, water-soluble Na-polyP. Heating the printed scaffold to 700 °C causes condensation of NaH₂PO₄, resulting in the formation of a Na-polyP glass melt that embeds the Ca-polyP-NP and<i>β</i>-TCP particles. The final scaffolds exhibited the necessary porosity, with pore sizes ranging from 10 to 100 µm (average 84 µm), which are suitable for bone ingrowth, along with the required mechanical stability. The morphogenetically active polyP component is released from the 3D-printed porous scaffolds in appropriate amounts, significantly increasing both the proliferation and energy-dependent differentiation of mesenchymal stem cells (MSCs) into mineralizing osteoblasts compared to polyP-free<i>β</i>-TCP scaffolds. Moreover, enhanced formation of collagen fibers and hydroxyapatite deposits on the cell surface, as well as accelerated microvessel tube formation, were observed in MSCs seeded on polyP-containing scaffolds. These results d`emonstrate that the novel strategy of integrating<i>β</i>-TCP with polyP as an energy-supplying, regeneration-promoting component imparts superior functional properties to<i>β</i>-TCP scaffolds, making them a promising material for future bone implant applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Doxorubicin and disulfiram metabolite encapsulated biomimetic liposomal formulation as an effective combination therapy against leukaemia.","authors":"Urooba Tariq, Nosheen Fatima Rana, Mariam Anees, Sabah Javaid, Tahreem Tanweer, Usama Sabir","doi":"10.1088/1748-605X/ae0554","DOIUrl":"https://doi.org/10.1088/1748-605X/ae0554","url":null,"abstract":"<p><p>Leukaemia is a haematopoietic system malignancy depicted by the infiltration of the bone marrow, blood and other tissues by proliferative and abnormally differentiated cells of the haematopoietic system. The available therapies aim to induce cell death of these poorly differentiated cells by various means. The anthracycline doxorubicin (DOX) regime remains the standard first-line treatment for leukaemia. DOX has potent anticancer activity at higher dosage concentration and imparts cardiac, renal and hepatic toxicity. The disulfiram metabolite complex zinc diethyldithiocarbamate (Zn-DDC) has potent anticancer efficacy; however, it has a short half-life due to its instability in gastric juice and the blood stream. The present study employed a thin-film hydration method to synthesise liposomal nanoparticles encapsulating DOX (DOX-NPs), Zn-DDC (Zn-DDC-NPs) and both Zn-DDC and DOX (Zn-DDC + DOX-NPs).<i>In vitro</i>cytotoxicity and antioxidant assays were performed to assess their cytotoxicity and antioxidant activity. The liposomes were evaluated against leukaemia in Wistar rats. After leukaemia induction through benzene, haematological and serological assays, morphological and histological examinations were conducted to evaluate treatment approaches. All liposomal formulations overcame their limitations, improved the blood parameters (<i>p</i>> 0.05), restored the hepatic and renal enzyme levels (<i>p</i>> 0.05), and reduced the blast cells in blood and tissues. However, in co-encapsulated liposomes, Zn-DDC reduced the cytotoxicity caused by DOX and provided results more analogous to normal.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional SISTP dressing integrating AI-screened hexapeptide for sustained antimicrobial release and redox homeostasis in infected wounds.","authors":"Youjia Yue, Huifeng Liu, Ying Wang","doi":"10.1088/1748-605X/ae0ef4","DOIUrl":"https://doi.org/10.1088/1748-605X/ae0ef4","url":null,"abstract":"<p><p>Infected wound healing environments present dual challenges of microbial colonization and sustained oxidative stress, critically impairing patient outcomes. Developing advanced dressings capable of concurrent broad-spectrum antimicrobial action and redox homeostasis restoration remains an urgent clinical priority. Here, we engineered a multifunctional porcine small intestinal submucosa extracellular matrix dressing (i.e., SISTP) integrated with AI-screened antimicrobial peptides (AMPs) via tea polyphenol-mediated coordination. The CRRI6 hexapeptide (Arg-Trp-Trp-Arg-Trp-Phe) demonstrated prolonged release kinetics (>6 hours) from the SISTP scaffold, achieving ≥90% eradication of Escherichia coli and Staphylococcus aureus. Radical scavenging assays confirmed SISTP's capacity to neutralize reactive oxygen species (ROS), while in vivo studies revealed accelerated wound closure in infected rat models through synergistic microbial clearance and oxidative stress mitigation. This study pioneers a bioinspired strategy leveraging AI-optimized AMPs and polyphenol nanoengineering to address the multifactorial pathophysiology of chronic wounds.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological performance of ZnO-doped hydroxyapatite coatings on PEEK: In vitro antibacterial, cytotoxic, and osteogenic assessment for orthopedic implants.","authors":"Jun Xu, Svea Sachse, Hanen Ferjani, Andreas Pfuch, Cornelia Wiegand, Thomas Lampke","doi":"10.1088/1748-605X/ae0ee9","DOIUrl":"https://doi.org/10.1088/1748-605X/ae0ee9","url":null,"abstract":"<p><p>ZnO-doped hydroxyapatite (HAp) coatings were developed on thermally sensitive polyetheretherketone (PEEK) substrates using a hybrid plasma spraying approach that combines powder and solution precursor feedstocks. Three coating architectures with different ZnO contents were designed to assess the influence of zinc incorporation on antibacterial and osteogenic performance. All coatings were deposited at a low plasma power (5.7 kW), enabling successful deposition without thermal degradation of the PEEK substrate, and achieving bond strengths up to 17 MPa. ZnO-doped coatings exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus, with significantly higher efficacy against E. coli. In vitro tests using MC3T3-E1 pre-osteoblasts showed enhanced cytocompatibility and osteogenic differentiation at low ZnO concentrations, as indicated by increased alkaline phosphatase (ALP) activity and calcium deposition exceeding those of undoped HAp coatings by over 50% after 21 days. The combination of antimicrobial and osteoinductive properties suggests that ZnO-doped HAp coatings are promising candidates for PEEK-based orthopedic implants.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential of Stent Cell Geometry to Affect Endothelialisation Performance: A Review of Existing Research and Future Perspective.","authors":"Kuang Yee Ng, Noorhafiza Muhammad, Mohd Shuhidan Saleh, Siti Noor Fazliah Mohd Noor, Nur Amalina Muhammad, Kamalakanta Muduli, V K Bupesh Raja, Kah Vui Chong","doi":"10.1088/1748-605X/ae0dd2","DOIUrl":"https://doi.org/10.1088/1748-605X/ae0dd2","url":null,"abstract":"<p><p>Endothelialisation is critical for the success of coronary stents, as it mitigates thrombosis risk and ensures long-term vascular healing. While advancements in stent materials, surface modifications and surface coatings have improved stent performance, the influence of stent cell geometry (particularly cell shape and size) on endothelialisation remains underexplored. This review examines the principles of cell growth influenced by geometry, drawing insights from non-coronary stent applications to identify research gaps in coronary stent applications. While recent studies highlight the role of surface microstructure in endothelialisation, the impact of stent cell geometry remains largely unexplored. Moreover, insights from tissue engineering (TE) suggest that optimising scaffold geometry could enhance endothelial cells (ECs) adhesion and proliferation, thereby accelerating re-endothelialisation. Based on these considerations, this review hypothesizes that optimising stent cell geometry could directly regulate ECs behaviour, thereby influencing endothelialisation performance. Finally, this paper critically evaluates the limitations of existing research and proposes future directions for leveraging cell geometry in the development of next-generation stents with improved biocompatibility and endothelialisation performance.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145202247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-bioprinted cell-laden bilayered chitosan scaffolds with decorin: a novel approach to mimicking skin architecture.","authors":"Afsaneh Ehsandoost, Tero Järvinen, Elnaz Tamjid","doi":"10.1088/1748-605X/ae0549","DOIUrl":"10.1088/1748-605X/ae0549","url":null,"abstract":"<p><p>It is essential to develop new strategies for wound treatment and skin reconstruction, particularly by scaffolds that replicate the structure and function of native skin. A bilayer scaffold was developed using three-dimensional bioprinting, based on a uniform chitosan-based formulation for both layers, maintaining material uniformity while offering structural support and promoting cell adhesion. The upper chitosan layer, embedded with Newborn Human Epidermal Keratinocytes-Neo, is stiffer and mimics the epidermis, while the softer lower layer contains embedded HFFs and HFSCs, mimicking the dermis. Moreover, the softer layer was infused with recombinant decorin (DCN) proteoglycans for skin repair through controlled release. The scaffold facilitates effective fluid management. Its positive contact angle suggests sufficient wettability. The scaffold layers have high water content and swelling capacity. The epidermis displayed lower compressive strength due to its more protective and less hydrated nature. Rheological analysis confirmed the scaffold's viscoelastic behavior. Chitosan-gel had high cytocompatibility. Chitosan scaffolds supplemented with DCN proteoglycans had enhanced blood entrapment and clotting. The scaffold's timely biodegradation may reduce prolonged material exposure and support safe tissue integration. This scaffold has potential in the treatment of acute and chronic wounds.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROS-driven, p53-mediated apoptosis in HepG2 cells induced by<i>Blumea eriantha</i>carbon quantum dots.","authors":"Pallavi Salve, Somnath Bhinge","doi":"10.1088/1748-605X/ae079e","DOIUrl":"10.1088/1748-605X/ae079e","url":null,"abstract":"<p><p>Carbon quantum dots (CQDs), owing to their small size, special surface functionalities, and remarkable fluorescence properties, have gained significant attention from researchers in the biomedical field. In the present work, CQDs were synthesized from<i>Blumea eriantha</i>DC (BEDC) extract using green approach via microwave-assisted technique. The synthesized BEDC-CQDs were characterized using spectroscopic techniques to confirm their formation. Strong absorption peaks at 279.46 nm and 325.41 nm are attributed to the excitation of<i>π</i>and<i>n</i>electrons of C=C and C=O groups, respectively, indicating the formation of CQDs. HepG2 cells were treated with varying concentrations of BEDC-CQDs and gauged via MTT assay, flow cytometry, and western blot analysis. Reactive oxygen species (ROS) generation, and expression of p53 and MDM2 proteins were evaluated to determine the cytotoxic mechanism. BEDC-CQDs exhibited bright light-blue fluorescence under UV irradiation, with photoluminescence quantum yield 18.90%. X-ray diffraction peaks reveal the nano-crystalline nature of the BEDC-CQDs. High-resolution transmission electron microscopy analysis revealed that BEDC-CQDs are spherical particles with sizes ranging from 2.19 to 8.95 nm. The MTT assay of BEDC-CQDs on HepG2 cells demonstrated substantial cell cytotoxicity at a concentration of 50 μg ml^-1, with an IC₅₀value of 40.86 μg ml^-1. Flow cytometry results indicated that BEDC-CQDs induced apoptosis in HepG2 cells. Intracellular ROS levels were also found to be significantly increased in HepG2 cells after treatment with BEDC-CQDs. Western blot analysis further disclosed that the expression of p53 and MDM2 were increased by 6.282- and 3.836-fold, respectively, in BEDC-CQD treated HepG2 cells compared to the control. These observations suggest that the synthesized BEDC-CQDs could serve as a viable therapeutic agent against hepatocellular carcinoma and support further exploration of similar nanohybrids with other bioactive compounds.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145076676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}