Biomedical materials (Bristol, England)最新文献

{"title":"The enhanced osteogenesis of calcium phosphate oligomers modified collagen membrane to guide bone regeneration.","authors":"Xiaoying Xu, Jiuzhou Dong, Hongkun Chen, Shuhang Hu, Cen Chen, Fan Liu","doi":"10.1088/1748-605X/adf386","DOIUrl":"10.1088/1748-605X/adf386","url":null,"abstract":"<p><p>Guided bone regeneration (GBR) is a promising technology for enhancing osteogenesis while preventing the invasion of fibrous tissue in implantation. Although collagen membranes have been widely utilized in GBR applications, their ability to support sufficient bone formation remains limited. Herein, we developed collagen-sodium alginate membranes (CSaM) with ultrasmall calcium phosphate oligomers (CPOs) incorporation by either physical adsorption (CSaM-em) or coprecipitation (CSaM-im). These two forms of organic-inorganic interaction facilitated biomimetic mineralization<i>in situ</i>, exhibiting high hydrophilicity, proper degradable rate, good mechanical properties, and favorable biocompatibility. Furthermore,<i>in vivo</i>test illustrated that CSaM-im membrane exhibited superior bone formation ability. These results suggested that CSaM with CPOs coprecipitation enhanced physicochemical properties and improved osteogenesis, highlighting their significant potential for applications in GBR.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144700472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-responsive nanomedicines for cancer therapy: design principles and therapeutic advances.","authors":"Yan Liu, Yinan Wang, Ahequeli Gemingnuer, Hailing Wang, Xin Meng","doi":"10.1088/1748-605X/adf387","DOIUrl":"https://doi.org/10.1088/1748-605X/adf387","url":null,"abstract":"<p><p>Tumor microenvironment (TME)-responsive nanomedicines have emerged as a promising precision therapeutic strategy in cancer treatment. By incorporating stimuli-responsive properties, these nanomedicines can achieve targeted delivery and controlled release at tumor sites, thereby enhancing therapeutic efficacy while minimizing side effects. This review provides a comprehensive overview of the latest advancements in TME-responsive nanomedicines for cancer immunotherapy, covering various stimulus-responsive mechanisms (such as pH, reactive oxygen species, hypoxia, enzymes, and ATP) and their applications in improving immune efficacy and reducing immune-related adverse effects. In addition to discusses the key challenges associated with the clinical translation of these nanomedicines and proposes future research directions. This work aims to offer a theoretical foundation and design reference for the further development and application of tumor-responsive nanomedicines.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schiff base formed functional hydrogel dressing via ε-poly-L-lysine modified chitosan and oxidized dextran with the incorporation of epigallocatechin-3-gallate.","authors":"Lei Nie, Xinran Li, Taiping Zhang, Yuanyuan Lu, Peng Ding","doi":"10.1088/1748-605X/adf67b","DOIUrl":"https://doi.org/10.1088/1748-605X/adf67b","url":null,"abstract":"<p><p>Hydrogel-based functional dressings for wound healing have garnered increasing attention due to their excellent hydrophilicity, adjustable mechanical properties, and superior biocompatibility. In this study, a composite hydrogel was facilely fabricated through the Schiff base reaction between ε-poly-L-lysine modified chitosan (CS-PL) and oxidized dextran (Odex). The formed hydrogel displayed the interconnected microstructure (100-200 μm), injectability, and adjustable mechanical properties. Macroscopic observation and alternating strain rheological analysis confirmed the good self-healing ability of the hydrogel. Furthermore, with the incorporation of epigallocatechin-3-gallate (EGCG), the composite hydrogel exhibited an improved reactive oxygen species (ROS) scavenging capability and good antibacterial activity against E. coli and S. aureus. The designed composite hydrogel dressings exhibited hemolysis rates of 0.75 ± 0.60% to 0.81 ± 0.31%, indicating their excellent hemocompatibility. Moreover, CCK-8 analysis and fluorescent images confirmed the excellent cytocompatibility of the hydrogels after co-culturing with NIH 3T3 cells for various periods. The above results offer a promising strategy for preparing functional hydrogel dressings via ε-poly-L-lysine modification on chitosan for wound healing applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inorganic whiskers containing Alkaline and Bioactive Ions enhance the comprehensive properties of 3D-printed Biopolymer Bone Scaffold.","authors":"Sidan Feng, Bowen Li, Yanting Wei, Yunlei Wu, Yanjian Huang, Bin Liu, Shan Wang, Huaming Mai, Wenjie Zhang, Hui You, Jieming Wen, Yu Long, Wang Guo","doi":"10.1088/1748-605X/adf619","DOIUrl":"https://doi.org/10.1088/1748-605X/adf619","url":null,"abstract":"<p><p>Polylactic acid (PLA) has been widely studied as a scaffold material for bone tissue engineering but still faces challenges such as insufficient mechanical strength, slow degradation rate, and poor biomineralization and cellular response. In this study, composite bone scaffolds were fabricated by fused deposition modeling (FDM) 3D printing with different contents of basic magnesium sulfate whiskers (BMSW) (0, 2.5, 5.0, 7.5, and 10wt%) incorporated into PLA. The compression properties of the scaffolds improved with increasing BMSW content and peaked at 5wt% BMSW, with the strength and modulus reaching 21.51 MPa and 297.38 MPa, respectively, 73% and 50% higher than those of PLA due to the reinforcing effect and uniform distribution of BMSW whiskers. The addition of BMSW accelerated the degradation of the PLA scaffold, with faster degradation observed at higher BMSW contents. Specifically, the alkaline ions (such as OH⁻) released by BMSW neutralized the acidic products generated during the degradation of PLA, thereby accelerating the degradation of the scaffold through the synergistic effect of acid and base. Magnesium ions released during BMSW degradation were steadily released due to the encapsulation effect of the PLA matrix, and their concentration could be controlled by varying the BMSW content. The incorporation of BMSW also enhanced the biomineralization capacity of the composite scaffolds in simulated body fluid and promoted the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, as confirmed by fluorescence and alkaline phosphatase (ALP) staining. This study demonstrates that incorporating inorganic whiskers containing bioactive and alkaline ions into PLA can enhance its overall performance, making it more suitable for bone scaffold development.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144755180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERS based pH nanosensors for in-vitro pH measurement in multicellular 3D Tumour spheroids.","authors":"Koyel Dey, Venkanagouda S Goudar, Tuhin S Santra, Fan-Gang Kevin Tseng","doi":"10.1088/1748-605X/adf47f","DOIUrl":"https://doi.org/10.1088/1748-605X/adf47f","url":null,"abstract":"<p><p>Tumor acidosis is a consequence of altered metabolism that, primarily takes place due to lactate secretion from anaerobic glycolysis. As a result, many regions within the tumors are chronically hypoxic and acidic. Here, we have fabricated a biocompatible pH nanoparticle sensor using surface-enhanced Raman spectroscopy (SERS-pNPS) and monitored continious pH levels in three-dimensional multicellular spheroids. The 3D multicellular spheroids were cultured using a micro-well array chip made of polydimethylsiloxane (PDMS). The SERS-pNPS were synthesized by linking 4-Mercaptobenzoic acid (4-MBA) to silver nanoparticles (AgNPs) of size 50 nm. The calibration curve demonstrates a linear correlation between the ratio of Raman peak intensities (1378 cm-1 / 1620 cm-1) with the pH level. The sensor exhibits a detection limit of pH 4.4 and demonstrates linearity within the physiological pH range (pH 4.4 - pH 8.23). The SERS-pNPS was applied for pH measurement in different 3D co-cultured spheroid models such as lung cancer (A549-NIH3T3), breast cancer (MCF-NIH3T3), colon cancer (HCT8-NIH3T3) and mono-cultured spheroids using fibroblast (NIH3T3) cells. The detailed analysis indicated that the 3D co-cultured cancerous tumour models have 16% more acidic microenvironment as compared to 3D mono-cultured spheroid model. Also, a presence of a decreasing pH gradient from peripheral to the core region is observed in both the cases indicating acidosis in the core region. The SERS-pNPS platform facilitates a real-time pH tracking, and thus offers an improved insight into the acidic microenvironment in various tumor models.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144719229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A brief overview of hyaluronic acid research and its applications in cell culture.","authors":"Huanyu Zhu, Na Zhao, Xueping Guo","doi":"10.1088/1748-605X/adecd1","DOIUrl":"10.1088/1748-605X/adecd1","url":null,"abstract":"<p><p>Hyaluronic acid (HA) is a large molecular acidic glycosaminoglycan that is widely present in the extracellular matrix (ECM) of cells. Aside from being an important component of the ECM, HA is an excellent biomaterial that is known for its good biocompatibility and mechanical properties. This study aimed to investigate the employment and effectiveness of HA in cell culture. We performed a review on the physicochemical properties of HA and its application in cell culture. HA is widely used in a number of<i>in vitro</i>cell cultures, especially in stem cell cultivation and differentiation and in tissue-engineering applications, which has greatly expanded the scope and field of its applications. This article provides a brief overview of the applications of HA in various cell culture fields, thereby providing a reference for related research on HA.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhance osteogenesis of BCP bioceramics by autocrine induced membrane to bone regeneration.","authors":"Xiaohua Wang, Yuehao Wu, Aiai Li, Zhao Xie, Wei Zhi, Wei Lin, Guanglin Wang","doi":"10.1088/1748-605X/adf022","DOIUrl":"10.1088/1748-605X/adf022","url":null,"abstract":"<p><p>This study investigates a novel strategy combining biphasic calcium phosphate (BCP) bioceramics with autocrine induced membranes (IMs) to enhance osteogenesis and vascularization for bone regeneration. Highly bioactive, porous BCP scaffolds (porosity: 68.1 ± 1.7%; pore size: 526-1000 µm) were combined with autocrine membranes in a rat femoral defect model. The optimal membrane formation time was determined by ELISA analysis of osteogenic and angiogenic factors (BMP-2, VEGF, ANG-II, PEG-2, FGF-2). Material characterization included SEM, XRD, and mercury intrusion porosimetry.<i>In vivo</i>bone regeneration was evaluated via micro-CT, histological analysis, and osteogenic marker expression (Alp, Bmp2, Col-1, Ocn, Opn, Runx2). The 4-week autocrine membrane exhibited superior osteogenic and angiogenic activity. Combined with BCP scaffolds, it accelerated bone regeneration, with micro-CT and histology showing significant new bone formation by 3 weeks and near-complete defect repair by 6 weeks. Osteogenic gene/protein expression (Alp, Bmp2, Col-1, Ocn, Opn, Runx2) was consistently higher in the BCP + IM group (BCP bioceramics with autocrine IMs) when compared to that of the BCP group and the control group, corroborating histological outcomes. Autocrine IMs significantly enhance the osteogenic efficacy of BCP bioceramics, demonstrating promise for weight-bearing bone defect repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly (ortho esters) (POEs) as cutting-edge biodegradable polymers for targeted cancer treatment and overcoming multidrug resistance.","authors":"Devansh Shah, Sankha Bhattacharya","doi":"10.1088/1748-605X/adefa7","DOIUrl":"10.1088/1748-605X/adefa7","url":null,"abstract":"<p><p>Poly (ortho esters) (POEs), biodegradable polymers featuring acid-labile ortho ester bonds formed through diol-diketene acetal reactions, are transforming cancer treatment with pH-sensitive surface erosion. This analysis explores the development of POE I, II, III, and IV (POE I-IV), suggesting that their adjustable degradation and controlled drug release may address multidrug resistance (MDR) and transform targeted cancer treatment. We seek to highlight the structural adaptability of POEs, their therapeutic functions, and their potential as advanced drug delivery systems. POE I, developed in the 1970s, faced challenges with autocatalytic degradation. POE II brought in neutral byproducts for enhanced stability, POE III facilitated injectable semi-solid formulations, and POE IV, the ultimate advancement, incorporates latent acid segments for self-catalysed hydrolysis in acidic tumour micro environments (pH 6.5-6.8), removing the need for external excipients. POE nanoparticles (50-300 nm) flexibly modify their size to improve tumour infiltration through the enhanced permeability and retention effect. Surface alterations, such as PEGylation or ligand attachment (e.g. folic acid), enable accurate targeting while minimising systemic toxicity. POEs are proficient in jointly delivering chemotherapeutics and immunomodulators, addressing MDR by inducing apoptosis, necrosis, autophagy, and pyroptosis, enhancing anti-tumour immunity. The degradation products that are biocompatible, such as acids and alcohols, promote immune interaction within the tumour microenvironment (TME). The review examines the synthesis, characterisation, and applications of POEs in post-surgical chemotherapy, ocular oncology, and protein delivery, as well as their interactions with cancer cell membranes and modulation of the TME. Issues such as scalability in manufacturing, enduring biocompatibility, and regulatory challenges are tackled, along with POEs' promise in immunotherapy and gene editing for tailored medicine. Through the integration of these insights, we emphasise POEs as a symbol of optimism for targeted, less harmful cancer therapies, leading to groundbreaking oncology advancements.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering polystyrene microtube-embedded composite hydrogels for tunable vascular morphogenesis.","authors":"Xianyang Li, Sadia Khan, Liyuan Wang, Yan Chen, Xiang Fang, Yingge Zhou, Ying Wang","doi":"10.1088/1748-605X/adebd0","DOIUrl":"10.1088/1748-605X/adebd0","url":null,"abstract":"<p><p>Establishing functional vascular systems within three-dimensional tissue constructs is crucial for their successful use in disease modeling, drug testing, and regenerative medicine. Current methods face challenges in creating small- to medium-sized microvessels and precisely controlling key vascular features, such as vascular density, vessel diameter, and network connectivity, to generate hierarchical, multiscale vascular systems that mimic natural functionality. In this study, we developed a composite hydrogel incorporating polystyrene microtubes (PS-MTs) to improve control over microvessel morphogenesis and functionality. PS-MTs were fabricated via core-sheath electrospinning, fragmented by ultrasonication, and incorporated into fibrin gels. Scanning electron microscopy revealed both micro- and nano-topographic features of the embedded PS-MT fragments. Endothelial cells (ECs) and fibroblasts were cocultured in this composite hydrogel under interstitial flow conditions for 7 d. The PS-MTs exhibited excellent biocompatibility, and the composite hydrogel showed no adverse effects on the cell viability of EC-fibroblast cocultures on chip. Fluorescence and confocal microscopy revealed a 40% increase in vascular area fraction and more than a twofold increase in average vessel diameter in the high PS-MT density group (>4%) compared to controls. Perfusion assays using a fluorescent microbead suspension demonstrated a 71% increase in the field-average speed of microbead flow, indicating enhanced perfusability, consistent with the observed morphological changes. Additionally, permeability assays showed a 66% decrease in dextran permeability, suggesting improved vascular barrier integrity. In conclusion, incorporating PS-MTs into fibrin hydrogels effectively modulated the structural organization and functional maturation of microvascular networks in a dose-dependent manner. This strategy holds promises for advancing the biofabrication of functional, multiscale vascular networks for engineered tissues. By tuning PS-MT density within the composite hydrogel, this approach enables local modulation of vessel morphogenesis, offering a flexible strategy for engineering application-specific vascular architectures.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximising adherent cell production via customisable and dissolvable bio-polymer microcarriers.","authors":"Timothy R Cox, David Lesmana, Christopher J O'Keeffe, Alan Tin Lun Lam, Weibin Zou, Zidong Lin, Xuye Lin, Thomas H Roberts, Khoon S Lim, Steve Kw Oh, Payar Radfar, Majid Ebrahimi Warkiani, Lin Ding","doi":"10.1088/1748-605X/adf1cd","DOIUrl":"https://doi.org/10.1088/1748-605X/adf1cd","url":null,"abstract":"<p><p>Large-scale cellular production systems offer a significant and diverse benefit impacting the therapeutic (stem cell and vaccine production) and cellular agriculture (lab-grown meat) sectors. Producing desired cells at mass can improve production yield whilst reducing the environmental and ethical burden associated with industrialised agriculture and production of therapeutic goods. Many existing large-scale cultivation strategies of adherent cells leverage the use of microcarriers (MCs) within bioreactors. However, currently commercial MCs are not dissolvable and lack specificity for different cell types and bioprocessing contexts.&#xD;In this work, we validate the effectiveness of customisable, polymeric MCs engineered to enhance cell growth and productivity. These MCs, which can be adjusted in terms of stiffness, surface charge, and size, maintain their structural integrity while offering precise property modifications. Under specific bioprocessing conditions, the custom MCs demonstrated significant improvements in cell productivity and sustainability compared to other commercial options. Our study (1) highlights how tailored substrate properties, particularly stiffness, can significantly impact cell yield and outcomes, and (2) suggests additional optimisations in surface charge and size that could further enhance MC technology. These advancements have the potential to improve large-scale cell and virus production efficiency, ultimately reducing the cost of production.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144669100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}