Biomedical materials (Bristol, England)最新文献

{"title":"3D printed magnetoactive nanocomposite scaffolds for bone regeneration.","authors":"Yeganeh Kaviani, Hossein Eslami, Mojtaba Ansari, Seyed Ali Poursamar","doi":"10.1088/1748-605X/ad9f04","DOIUrl":"10.1088/1748-605X/ad9f04","url":null,"abstract":"<p><p>Simulating the natural cellular environment using magnetic stimuli could be a potential strategy to promote bone tissue regeneration. This study unveiled a novel 3D printed composite scaffold containing polycaprolactone (PCL) and cobalt ferrite/forsterite core-shell nanoparticles (CFF-NPs) to investigate physical, mechanical and biological properties of magnetoactive scaffold under static magnetic field. For this purpose, core-shell structure is synthesized through a two-step synthesis strategy in which cobalt ferrite nanoparticles are prepared via sol-gel combustion method and then are coated through sol-gel method with forsterite. The characterization regarding CFF-NPs reveals that Mg₂SiO₄-coated CoFe₂O₄nanoparticles is successfully synthesized with a core-shell structure. Afterwards, CFF-NPs are embedded within the PCL with different percentages, ultimately 3D printed scaffolds were fabricated. The<i>in vitro</i>assessments demonstrated that the incorporated CFF-NPs are able to cause a decrease in contact angle which was responsible for modulating purposefully the degradation rate of PCL scaffold, resulting in providing the obligatory environment for bone growth. In addition, it was observed that scaffolds including PCL combined with CFF-NPs are susceptible to improve the mechanical performance of nanocomposite scaffolds, up to a certain concentration (50% CFF-NPs and 50% PCL) with compressive modulus of 42.5 MPa. Moreover, when being exposed to simulated body fluid (SBF) solution, hydroxyapatite deposition on the surface of scaffolds was observed. Thus, these compositions may be useful for improving the osteointegration between the implant and bone tissue after implantation. Finally, the simultaneous effect of magnetic nanoparticles and magnetic field of 125 mT evaluated on cellular behavior of scaffolds. The results showed that the cell viability of all groups under magnetic field were better than that for standard condition. Likewise, SEM images of cultured cells on scaffolds confirmed that the combined effect of these factors could be lead to promote better cell adhesion, dispersion, and bone regeneration.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carboxyl-functionalized multifunctional red-emitting carbon quantum dots as an ideal biomaterial.","authors":"Xi Wen, Chen Wang, Xinyu Liang, Shengxiu Liu","doi":"10.1088/1748-605X/ad9f05","DOIUrl":"10.1088/1748-605X/ad9f05","url":null,"abstract":"<p><p>Carbon quantum dots (CQDs) have been developed into a popular nanomaterial due to their abundant surface state, good biocompatibility, and excellent antimicrobial properties. However, CQDs with multiple functions, such as being red-emitting, having good antibacterial activity, and having excellent pH sensitivity, have rarely been reported. In this work, red-emitting CQDs (R-CQDs) with excellent optical properties and antimicrobial activity were prepared by a simple green hydrothermal method. In antimicrobial applications, the R-CQDs featured good antibacterial activity due to the generation of reactive oxygen species, indicating excellent photodynamic antimicrobial ability. In addition, the R-CQDs showed fine pH sensitivity, giving them potential as pH sensors to monitor the pH of wounds in real time. The promising potential application of R-CQDs for cell imaging was also demonstrated. In summary, we offer R-CQDs with good antibacterial and pH sensitivity as a potential nanomaterial for pH and antimicrobial monitoring of wounds, shedding light on the biomedical field.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4D printing of smart scaffolds for bone regeneration: a systematic review.","authors":"Niusha Gharehdaghi, Hanieh Nokhbatolfoghahaei, Arash Khojasteh","doi":"10.1088/1748-605X/ad8f80","DOIUrl":"10.1088/1748-605X/ad8f80","url":null,"abstract":"<p><p>As a novel emerging technology, four-dimensional (4D) printing allows the stimulation of 3D-printed materials in order to change shape, color, functionality, etc, over time. This systematic review is conducted to evaluate the purpose, materials, physiomechanical, and biological properties of 4D-printed scaffolds used for bone tissue engineering. An electronic search was conducted following the PRISMA 2020 guidelines in PubMed, Scopus, Web of Science, and Google Scholar online databases limited to English articles until April 2024. Studies in which scaffolds were fabricated through 3D printing methods responding to external stimulation were included. The quality of<i>in vitro</i>and<i>in vivo</i>studies was evaluated through the modified CONSORT checklist and SYRCLE's risk of bias tool. The full text of 57 studies were reviewed, and 15 studies met the inclusion criteria. According to the analyzed studies, most scaffolds responded to temperature changes showing shape memory effect. Polyurethane and poly(lactic acid) were the most common shape memory polymers (SMPs), and the most common fabrication method used was fused deposition modeling. A comprehensive systematic review of the studies from the past 10 years demonstrated several findings: (1) Shape memory, drug delivery, and shape morphing are three general purposes of 4D printing for bone regeneration. (2) Smart materials used for 4D printing mostly consist of SMPs. (3) Temperature changes account for the majority of stimulation used for 4D printing. (4) incorporating 4D printing principles does not negatively impact on the physiomechanical properties of the designed scaffold. (5) The 4D-printed scaffolds show a higher osteogenic differentiation capacity than their identical 3D-printed structures in terms of bone regeneration.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142592405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterization of a novel photothermal hydrogel composite with combined osteogenic and antibacterial activities.","authors":"Tao Ge, Jianxiang Jin, Kuan Feng, Xialong Gu, Gengfan Ye, Taotao Shi, Jia Li, Hao Wang, Hongcai Wang, Maosong Chen","doi":"10.1088/1748-605X/ada2ce","DOIUrl":"https://doi.org/10.1088/1748-605X/ada2ce","url":null,"abstract":"<p><p>Cranial defect repair remains a significant challenge in neurosurgery, and designing material complexes that can support bone regeneration while minimizing complications such as infection and inflammation could help alleviate this clinical challenge. This study presents a photothermal hydrogel complex with a controlled rapid gelation process, PDA-G-A-H, which integrates photothermal polydopamine nanoparticles (PDA NPs) with gentamycin (G) and alendronate acid (A). Furthermore, the incorporation of the injectable hydrogel Pluronic F127 and collagen (H) made this composite hydrogel (PDA-G-A-H) suitable for the multifaceted needs of cranial defects. The PDA-G-A-H hydrogel exhibited superior biocompatibility, as evidenced by high cell viability and minimal hemolysis, making it a safe candidate for biomedical applications. In vitro assessments with MC3T3-E1 cells demonstrated that this hydrogel enhanced mineralization and osteogenic differentiation, and significant upregulation of key osteogenic markers was subsequently detected. The antibacterial activity of the hydrogel against Staphylococcus aureus and Staphylococcus epidermidis was also investigated. The results of the RT‒PCR analysis revealed the potential for inhibiting biofilm formation. The hydrogel composite combines biocompatibility, osteoinductive, and antibacterial potential. It has translational potential for cranial defect repair and other bone regeneration therapies.&#xD;Keywords: cranial defect, hydrogel, osteogenesis, antibacterial, biocompatibility, neurosurgery.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible TA4 and TC4ELI with excellent mechanical properties and corrosion resistance via multiple ECAP.","authors":"Liangzhong Li, Liyu Hao, Shuangle Zhang, Shangkun Shen, Xing Liu, Engang Fu","doi":"10.1088/1748-605X/ad9af0","DOIUrl":"https://doi.org/10.1088/1748-605X/ad9af0","url":null,"abstract":"<p><p>Titanium (Ti), characterized by its exceptional mechanical properties, commendable corrosion resistance and biocompatibility, has emerged as the principal functional materials for implants in biomedical and clinical applications. However, the Ti-6Al-4V (TC4ELI) alloy has cytotoxicity risks, whereas the strength of the existing industrially pure titanium TA4 is marginally inadequate and will significantly limit the scenarios of medical implants. Herein, we prepared ultrafine-grained industrial-grade pure titanium TA4 and titanium alloy TC4ELI via the equal channel angular pressing method, in which the TA4-1 sample has ultrahigh strength of 1.1 GPa and elongation of 26%. In comparison with the micrometer-crystalline Ti-based materials, it showed a 35% reduction in wear depth and more than 10% reduction in wear volume, while the difference in the corrosion potential of the simulated body fluids was not significant (only ∼20 mV). XRD, electron backscatter diffraction, and transmission electron microscope characterization confirms that their superior strengths are mainly due to grain refinement strengthening.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of biodegradable, antibacterial core-spun yarns with braided structures using QAS/PLA micro/nanocomposites.","authors":"Jun Xu, Xingyu Zhao, Zhenzhen Lei, Huimin Jia, Haolong He, Genghao Gong, Jing Wang, Tiegang Wang","doi":"10.1088/1748-605X/ada23d","DOIUrl":"https://doi.org/10.1088/1748-605X/ada23d","url":null,"abstract":"<p><p>Medical antibacterial textiles play a vital role in tackling the issue of bacterial infection. Traditional surgical sutures face significant challenges due to wound infection caused by bacteria and breakage and scars caused by poor suture strength. Therefore, a new antibacterial and high-strength suture preparation strategy with wide clinical applicability was highly desired. In this study, a biodegradable quaternary ammonium salt (QAS)/polylactic acid (PLA) core-spun yarn with excellent antibacterial and mechanical properties was prepared by conjugated electrospinning technology combined with the braiding process. The antibacterial test results revealed the best overall performance of the PLA micro/nanofiber core-spun yarn with 0.3 wt% QAS antibacterial agent. The antibacterial rate against Escherichia coli and Staphylococcus aureus was 94.49% and 94.00%, respectively, which could effectively solve the problem of wound infection caused by bacteria. In addition, we used the diamond-braided structure to address the poor strength and fragility of the traditional suture strength. The braiding angle of 30° and 45° could effectively enhance the mechanical properties of the yarn, and the breaking strength was also in line with the industry standard. The study proposed that the degradable QAS/PLA micro/nanofiber core-spun yarn, due to its excellent antibacterial and mechanical properties, could find application in medical protection. This provided a new avenue for research into new antibacterial surgical sutures.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimal parameter setting and evaluation for ultraviolet-assisted direct ink writing bioprinting of nHA/PEGDA scaffold.","authors":"Yumeng Li, Jiaqi Ma, Jing Wang, Yanlei Kong, Feng Wang, Pengfei Zhang, Fan Yawei","doi":"10.1088/1748-605X/ada241","DOIUrl":"https://doi.org/10.1088/1748-605X/ada241","url":null,"abstract":"<p><p>Ultraviolet-assisted Direct Ink Writing（UV-DIW）, an extrusion-based additive manufacturing technology, has emerged as a prominent 3D printing technique and is currently an important topic in bone tissue engineering research. This study focused on the printability of double-network (DN) bioink (Nano-hydroxyapatite/Polyethylene glycol diacrylate(nHA/PEGDA)). Next, we search for the optimal UV-DIW printing parameters for the scaffold formed by nHA-PEGDA. In the end, we developed a scaffold that has outstanding structural integrity and can repair bone defects. Achieving high-quality UV-DIW printing can be challenging due to a variety of factors (slurry solid content, rheology, printing conditions, etc.).At present, there are limited reports about precise parameter configurations for UV-DIW printing. We optimised the solid composition of the slurry by varying the quantities of nHA and PEGDA, establishing the maximum solid content (40 wt%) permissible for scaffold shaping. Consequently, we examined the influence of several factors (nozzle diameter, air pressure, and printing rate) on the surface morphology of the scaffolds and determined the ideal conditions to attain scaffolds with superior printing accuracy. The findings demonstrate excellent controllability, repeatability, and precision of the entire printing process. Finally, we evaluated the scaffolds that most effectively fulfilled the requirements for bone regeneration by examining their surface morphology and mechanical characteristics. The experimental findings indicate that nHA-PEGDA scaffolds fulfill the compressive strength requirements for bone tissue and possess promising applications in bone regeneration. This study demonstrates that the nHA-PEGDA bioink possesses significant potential as a scaffold material for bone tissue regeneration, exhibiting exceptional shape integrity and mechanical capabilities. The study found the optimal parameters for bio-3D printers and gave UV-DIW an exact data reference for making the nHA-PEGDA scaffold. In addition, it is a useful guide for 3D printing biomaterial scaffolds.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Encapsulation of AD-MSC- derived extracellular nanovesicles in an electrospun three-layer scaffold: characterization and controlled release analysis in vitro.","authors":"Raziyeh Ghorbani, Hojjat Allah Abbaszadeh, Reihaneh Ramezani, Niloofar Taghipour, Azam Rahimpour, Simzar Hosseinzadeh","doi":"10.1088/1748-605X/ada23f","DOIUrl":"https://doi.org/10.1088/1748-605X/ada23f","url":null,"abstract":"<p><p>The combining of therapeutic agents with electrospun nanofibers boosts their regeneration potential; therefore, Researchers have increasingly turned towards the development of electrospun nanofiber scaffolds to encapsulate or surface-adsorb biological payloads, such as cytokines, exosomes, peptides, nucleic acids, and enzymes. Due to their high surface-to-volume ratio, ease of manufacturing, and drug-loading capacity, electrospun nanofibers are hopeful in tissue engineering and scaffold fabrication. Electrospun multilayer scaffolds offer a promising construction for preserving the integrity and bioactivity of therapeutic factors while permitting the controlled and prolonged release of biomolecules into the environment. The present study aimed to evaluate the mechanism of controlled release of electrospun exosomes from a three-layer nanofiber scaffold and its effect on the expression of DDR2 and VEGF genes in fibroblast cells in vitro. Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) were obtained and isolated from liposuction surgery samples, and their intrinsic nature was confirmed using flow cytometry. After the exosomes were separated from the cell supernatant, their size, shape, and index markers were identified. The cytotoxicity, biocompatibility, and mechanical characteristics of scaffolds were evaluated. The qRT-PCR results showed the upregulation of DDR2 and VEGF genes in the three-layer scaffold containing the exosomes was 2.04 and 1.47-fold compared to the control group. The design and construction of multi-layered electrospun nanofibers loaded with bioactive substances and favorable mechanical and biological properties for controlled and sustained release will be promising and effective scaffolds for therapeutic purposes.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An injectable sodium-mangiferin homopolymeric hydrogel accelerated skin full-thickness wound healing in guinea pig model.","authors":"Ning-Xiang Wang, Mei-Rong Huang, Min Huang, Junmei Lyu, Pei-Ning Wei, Sun-Han Li, Shilei Ding, Tao Yang","doi":"10.1088/1748-605X/ada23c","DOIUrl":"https://doi.org/10.1088/1748-605X/ada23c","url":null,"abstract":"<p><p>In the treatment of joints, mucosa, and full-thickness wounds, traditional implant surgery presents not only inconvenience but also a significant risk of wound infection. Additionally, the pharmaceutical application of mangiferin(MGF) has been severely restricted due to its poor water solubility. In this study, we reported the synthesis and characterization of sodium-mangiferin (MGF-Na(S)) using the salt formation method. This novel compound exhibits a solubility of up to 80 mg/mL, which is remarkably 800 times higher than that of MGF. Subsequently, MGF-Na(S) was combined with water to synthesize an injectable sodium-mangiferin homopolymeric hydrogel (MGF-Na(HG)). The hydrogel was further characterized, and its wound-healing properties were investigated. The results indicated that MGF-Na(HG) effectively extends the residence time of therapeutic agents on the wound surface, thereby enhancing wound healing. Moreover, this hydrogel forms a protective gel layer that prevents exogenous bacterial reinfection, providing an optimal environment for wound healing. Furthermore, the hydrogel demonstrated excellent self-healing and injectable properties, highlighting its potential for managing postoperative wounds. The successful utilization of this injectable, self-healing, and antibacterial MGF-Na(HG) in wound healing offers a novel approach for the application of MGF.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From lab to life: advances in<i>in-situ</i>bioprinting and bioink technology.","authors":"Manav Sree Kumar, Payal Varma, Balasubramanian Kandasubramanian","doi":"10.1088/1748-605X/ad9dd0","DOIUrl":"10.1088/1748-605X/ad9dd0","url":null,"abstract":"<p><p>Bioprinting has the potential to revolutionize tissue engineering and regenerative medicine, offering innovative solutions for complex medical challenges and addressing unmet clinical needs. However, traditional<i>in vitro</i>bioprinting techniques face significant limitations, including difficulties in fabricating and implanting scaffolds with irregular shapes, as well as limited accessibility for rapid clinical application. To overcome these challenges,<i>in-situ</i>bioprinting has emerged as a groundbreaking approach that enables the direct deposition of cells, biomaterials, and bioactive factors onto damaged organs or tissues, eliminating the need for pre-fabricated 3D constructs. This method promises a personalized, patient-specific approach to treatment, aligning well with the principles of precision medicine. The success of<i>in-situ</i>bioprinting largely depends on the advancement of bioinks, which are essential for maintaining cell viability and supporting tissue development. Recent innovations in hand-held bioprinting devices and robotic arms have further enhanced the flexibility of<i>in-situ</i>bioprinting, making it applicable to various tissue types, such as skin, hair, muscle, bone, cartilage, and composite tissues. This review examines<i>in-situ</i>bioprinting techniques, the development of smart, multifunctional bioinks, and their essential properties for promoting cell viability and tissue growth. It highlights the versatility and recent advancements in<i>in-situ</i>bioprinting methods and their applications in regenerating a wide range of tissues and organs. Furthermore, it addresses the key challenges that must be overcome for broader clinical adoption and propose strategies to advance these technologies toward mainstream medical practice.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}