Biomedical materials (Bristol, England)最新文献_第9页

Harnessing extracellular vesicles-mediated signaling for enhanced bone regeneration: novel insights into scaffold design. 利用细胞外囊泡介导的信号传导促进骨再生：支架设计的新见解。

Biomedical materials (Bristol, England) Pub Date : 2024-07-04 DOI: 10.1088/1748-605X/ad5ba9

Hemalatha Kanniyappan, Varun Gnanasekar, Vincent Parise, Koushik Debnath, Yani Sun, Shriya Thakur, Gitika Thakur, Govindaraj Perumal, Raj Kumar, Rong Wang, Aftab Merchant, Ravindran Sriram, Mathew T Mathew

{"title":"Harnessing extracellular vesicles-mediated signaling for enhanced bone regeneration: novel insights into scaffold design.","authors":"Hemalatha Kanniyappan, Varun Gnanasekar, Vincent Parise, Koushik Debnath, Yani Sun, Shriya Thakur, Gitika Thakur, Govindaraj Perumal, Raj Kumar, Rong Wang, Aftab Merchant, Ravindran Sriram, Mathew T Mathew","doi":"10.1088/1748-605X/ad5ba9","DOIUrl":"10.1088/1748-605X/ad5ba9","url":null,"abstract":"The increasing prevalence of bone replacements and complications associated with bone replacement procedures underscores the need for innovative tissue restoration approaches. Existing synthetic grafts cannot fully replicate bone vascularization and mechanical characteristics. This study introduces a novel strategy utilizing pectin, chitosan, and polyvinyl alcohol to create interpenetrating polymeric network (IPN) scaffolds incorporated with extracellular vesicles (EVs) isolated from human mesenchymal stem cells (hMSCs). We assess the osteointegration and osteoconduction abilities of these modelsin vitrousing hMSCs and MG-63 osteosarcoma cells. Additionally, we confirm exosome properties through Transmission Electron Microscopy (TEM), immunoblotting, and Dynamic Light Scattering (DLS).In vivo, chick allantoic membrane assay investigates vascularization characteristics. The study did not includein vivoanimal experiments. Our results demonstrate that the IPN scaffold is highly porous and interconnected, potentially suitable for bone implants. EVs, approximately 100 nm in size, enhance cell survival, proliferation, alkaline phosphatase activity, and the expression of osteogenic genes. EVs-mediated IPN scaffolds demonstrate promise as precise drug carriers, enabling customized treatments for bone-related conditions and regeneration efforts. Therefore, the EVs-mediated IPN scaffolds demonstrate promise as precise carriers for the transport of drugs, allowing for customized treatments for conditions connected to bone and efforts in regeneration.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11305091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitroassessment of a gallium-doped glass polyalkenoate cement: chemotherapeutic potential, cytotoxicity and osteogenic effects. 掺镓玻璃聚烯酸酯水泥的体外评估：化疗潜力、细胞毒性和成骨效应。

Biomedical materials (Bristol, England) Pub Date : 2024-07-04 DOI: 10.1088/1748-605X/ad5ba5

Sunjeev Phull, Daniella Marx, Margarete K Akens, Michelle Ghert, Mark R Towler

{"title":"In vitroassessment of a gallium-doped glass polyalkenoate cement: chemotherapeutic potential, cytotoxicity and osteogenic effects.","authors":"Sunjeev Phull, Daniella Marx, Margarete K Akens, Michelle Ghert, Mark R Towler","doi":"10.1088/1748-605X/ad5ba5","DOIUrl":"10.1088/1748-605X/ad5ba5","url":null,"abstract":"Metastatic bone lesions are often osteolytic, which causes advanced-stage cancer sufferers to experience severe pain and an increased risk of developing a pathological fracture. Gallium (Ga) ion possesses antineoplastic and anti-bone resorption properties, suggesting the potential for its local administration to impede the growth of metastatic bone lesions. This study investigated the chemotherapeutic potential, cytotoxicity, and osteogenic effects of a Ga-doped glass polyalkenoate cement (GPC) (C-TA2) compared to its non-gallium (C-TA0) counterpart. Ion release profiles revealed a biphasic pattern characterized by an initial burst followed by a gradually declining release of ions. C-TA2 continued to release Ga steadily throughout the experimentation period (7 d) and exhibited prolonged zinc (Zn) release compared to C-TA0. Interestingly, the Zn release from both GPCs appeared to cause a chemotherapeutic effect against H1092 lung cancer cellsin vitro, with the prolonged Zn release from C-TA2 extending this effect. Unfortunately, both GPCs enhanced the viability of HCC2218 breast cancer cells, suggesting that the chemotherapeutic effects of Zn could be tied to cellular differences in preferred Zn concentrations. The utilization of SAOS-2 and MC3T3 cell lines as bone cell models yielded conflicting results, with the substantial decline in MC3T3 viability closely associated with silicon (Si) release, indicating cellular variations in Si toxicity. Despite this ambiguity, both GPCs exhibited harmful effects on the osteogenesis of primary rat osteoblasts, raising concerns about excessive burst Zn release. While Ga/Zn-doped GPCs hold promise for treating metastatic bone lesions caused by lung cancers, further optimization is required to mitigate cytotoxicity on healthy bone.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amorphous titanium oxide (aTiO₂) thin films biofunctionalized with CAP-p15 induce mineralized-like differentiation of human oral mucosal stem cells (hOMSCs). 用 CAP-p15 进行生物功能化的无定形氧化钛（aTiO2）薄膜可诱导人类口腔黏膜干细胞（hOMSCs）发生矿化样分化。

Biomedical materials (Bristol, England) Pub Date : 2024-07-04 DOI: 10.1088/1748-605X/ad5bab

Guadalupe Ureiro-Cueto, Sandra E Rodil, Phaedra Silva-Bermúdez, Maricela Santana-Vázquez, Lia Hoz-Rodríguez, Higinio Arzate, Gonzalo Montoya-Ayala

{"title":"Amorphous titanium oxide (aTiO2) thin films biofunctionalized with CAP-p15 induce mineralized-like differentiation of human oral mucosal stem cells (hOMSCs).","authors":"Guadalupe Ureiro-Cueto, Sandra E Rodil, Phaedra Silva-Bermúdez, Maricela Santana-Vázquez, Lia Hoz-Rodríguez, Higinio Arzate, Gonzalo Montoya-Ayala","doi":"10.1088/1748-605X/ad5bab","DOIUrl":"10.1088/1748-605X/ad5bab","url":null,"abstract":"Insufficient osseointegration of titanium-based implants is a factor conditioning their long-term success. Therefore, different surface modifications, such as multifunctional oxide coatings, calcium phosphates, and the addition of molecules such as peptides, have been developed to improve the bioactivity of titanium-based biomaterials. In this work, we investigate the behavior of human oral mucosal stem cells (hOMSCs) cultured on amorphous titanium oxide (aTiO2), surfaces designed to simulate titanium (Ti) surfaces, biofunctionalized with a novel sequence derived from cementum attachment protein (CAP-p15), exploring its impact on guiding hOMSCs towards an osteogenic phenotype. We carried out cell attachment and viability assays. Next, hOMSCs differentiation was assessed by red alizarin stain, ALP activity, and western blot analysis by evaluating the expression of RUNX2, BSP, BMP2, and OCN at the protein level. Our results showed that functionalized surfaces with CAP-p15 (1 µg ml-1) displayed a synergistic effect increasing cell proliferation and cell attachment, ALP activity, and expression of osteogenic-related markers. These data demonstrate that CAP-p15 and its interaction with aTiO2surfaces promote osteoblastic differentiation and enhanced mineralization of hOMSCs when compared to pristine samples. Therefore, CAP-p15 shows the potential to be used as a therapeutical molecule capable of inducing mineralized tissue regeneration onto titanium-based implants.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential use of extracted flax seed mucilage in the construction of macroporous cryo-scaffolds. 提取的亚麻籽粘液在构建大孔冷冻支架中的潜在用途。

Biomedical materials (Bristol, England) Pub Date : 2024-07-04 DOI: 10.1088/1748-605X/ad5bad

Didem Demir

{"title":"Potential use of extracted flax seed mucilage in the construction of macroporous cryo-scaffolds.","authors":"Didem Demir","doi":"10.1088/1748-605X/ad5bad","DOIUrl":"10.1088/1748-605X/ad5bad","url":null,"abstract":"Mucilage is a natural source of polysaccharides that has recently attracted attention for use in biomaterial production. It attracts attention with its easy and fast extraction, biocompatibility, high water retention capacity, and biodegradability. Although there are studies on the characterization of mucilage obtained from different plant sources, the interaction of this polymer with other polymers and its potential to form new biomaterials have not yet been sufficiently investigated. Based on this, in this study, the potential of mucilage extracted from flaxseed for the production of cryogels for tissue engineering applications was demonstrated. Firstly, yield, basic physicochemical properties, morphology, and surface charge-dependent isoelectric point determination studies were carried out for the characterization of the extracted mucilage. The successful preparation of mucilage was evaluated for the construction of cryo-scaffolds and 3D, spongy, and porous structures were obtained in the presence of chitosan and polyvinyl alcohol polymers. A heterogeneous morphology with interconnected macro and micro porosity in the range of approximately 85-115 m pore diameter was exhibited. Due to the high hydrophilic structure of the mucilage, which is attached to the structure with weak hydrogen bonds, the contact angle values of the scaffolds were obtained below 80° and they showed the ability to absorb 1000 times their dry weight in approximately 30 min. As a preliminary optimization study for the evaluation of mucilage in cryogel formation, this work introduced a new construct to be developed as wound dressing scaffold for deep and chronic wounds.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-parameter design of triply periodic minimal surface scaffolds: from geometry optimization to biomechanical simulation. 三重周期性最小表面支架的多参数设计：从几何优化到生物力学模拟。

Biomedical materials (Bristol, England) Pub Date : 2024-07-04 DOI: 10.1088/1748-605X/ad5ba8

Xiaoshuai Yang, Zhongwei Sun, Yuanbin Hu, Changwen Mi

{"title":"Multi-parameter design of triply periodic minimal surface scaffolds: from geometry optimization to biomechanical simulation.","authors":"Xiaoshuai Yang, Zhongwei Sun, Yuanbin Hu, Changwen Mi","doi":"10.1088/1748-605X/ad5ba8","DOIUrl":"10.1088/1748-605X/ad5ba8","url":null,"abstract":"This study introduces a multi-parameter design methodology to create triply periodic minimal surface (TPMS) scaffolds with predefined geometric characteristics. The level-set constant and unit cell lengths are systematically correlated with targeted porosity and minimum pore sizes. Network and sheet scaffolds featuring diamond, gyroid, and primitive level-set structures are generated. Three radially graded schemes are applied to each of the six scaffold type, accommodating radial variations in porosity and pore sizes. Computer simulations are conducted to assess the biomechanical performance of 18 scaffold models. Results disclose that diamond and gyroid scaffolds exhibit more expansive design ranges than primitive counterparts. While primitive scaffolds display the highest Young's modulus and permeability, their lower yield strength and mesenchymal stem cell (MSC) adhesion render them unsuitable for bone scaffolds. Gyroid scaffolds demonstrate superior mechanical and permeability performances, albeit with slightly lower MSC adhesion than diamond scaffolds. Sheet scaffolds, characterized by more uniform material distribution, exhibit superior mechanical performance in various directions, despite slightly lower permeability. The higher specific surface area of sheet scaffolds contributes to elevated MSC adhesion. The stimulus factor analysis also revealed the superior differentiation potential of sheet scaffolds over network ones. The diamond sheet type demonstrated the optimal differentiation. Introducing radial gradations enhances axial mechanical performance at the expense of radial mechanical performance. Radially decreasing porosity displays the highest permeability, MSC adhesion, and differentiation capability, aligning with the structural characteristics of human bones. This study underscores the crucial need to balance diverse biomechanical properties of TPMS scaffolds for bone tissue engineering.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141452333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of NIR-triggered PEG-coated NaGdF₄:Tm³⁺/Yb³⁺bio-compatible upconversion nanoparticles for contrast enhancement in OCT imaging and optical thermometry. 评估近红外触发 PEG 涂层 NaGdF4:Tm3+/Yb3+ 生物兼容上转换纳米粒子在 OCT 成像和光学测温中的对比度增强作用。

Biomedical materials (Bristol, England) Pub Date : 2024-07-03 DOI: 10.1088/1748-605X/ad580b

Kumar Shwetabh, Abhishek Banerjee, Raju Poddar, Kaushal Kumar

{"title":"Assessment of NIR-triggered PEG-coated NaGdF4:Tm3+/Yb3+bio-compatible upconversion nanoparticles for contrast enhancement in OCT imaging and optical thermometry.","authors":"Kumar Shwetabh, Abhishek Banerjee, Raju Poddar, Kaushal Kumar","doi":"10.1088/1748-605X/ad580b","DOIUrl":"10.1088/1748-605X/ad580b","url":null,"abstract":"In this investigation, we embarked on the synthesis of polyethylene glycol coated NaGdF4:Tm3+/Yb3+upconversion nanoparticles (UCNPs), aiming to assess their utility in enhancing image contrast within the context of swept source optical coherence tomography (OCT) and photo-thermal OCT imaging. Our research unveiled the remarkable UC emissions stemming from the transitions of Tm3+ions, specifically the1G4→3H6transitions, yielding vibrant blue emissions at 472 nm. We delved further into the UC mechanism, meticulously scrutinizing decay times and the nanoparticles' capacity to convert radiation into heat. Notably, these nanoparticles exhibited an impressive photo-thermal conversion efficiency of 37.5%. Furthermore, our investigations into their bio-compatibility revealed a promising outcome, with more than 90% cell survival after 24 h of incubation with HeLa cells treated with UCNPs. The nanoparticles demonstrated a notable thermal sensitivity of 4.7 × 10-3K-1at 300 K, signifying their potential for precise temperature monitoring at the cellular level.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whey protein-loaded 3D-printed poly (lactic) acid scaffolds for wound dressing applications. 用于伤口敷料的乳清蛋白负载 3D 打印聚（乳酸）支架。

Biomedical materials (Bristol, England) Pub Date : 2024-06-28 DOI: 10.1088/1748-605X/ad565d

Hanne Meryem Kayadurmus, Azadeh Rezaei, Elif Ilhan, Sumeyye Cesur, Ali Sahin, Oguzhan Gunduz, Deepak M Kalaskar, Nazmi Ekren

{"title":"Whey protein-loaded 3D-printed poly (lactic) acid scaffolds for wound dressing applications.","authors":"Hanne Meryem Kayadurmus, Azadeh Rezaei, Elif Ilhan, Sumeyye Cesur, Ali Sahin, Oguzhan Gunduz, Deepak M Kalaskar, Nazmi Ekren","doi":"10.1088/1748-605X/ad565d","DOIUrl":"10.1088/1748-605X/ad565d","url":null,"abstract":"Chronic skin wounds pose a global clinical challenge, necessitating effective treatment strategies. This study explores the potential of 3D printed Poly Lactic Acid (PLA) scaffolds, enhanced with Whey Protein Concentrate (WPC) at varying concentrations (25, 35, and 50% wt), for wound healing applications. PLA's biocompatibility, biodegradability, and thermal stability make it an ideal material for medical applications. The addition of WPC aims to mimic the skin's extracellular matrix and enhance the bioactivity of the PLA scaffolds. Fourier Transform Infrared Spectroscopy results confirmed the successful loading of WPC into the 3D printed PLA-based scaffolds. Scanning Electron Microscopy (SEM) images revealed no significant differences in pore size between PLA/WPC scaffolds and pure PLA scaffolds. Mechanical strength tests showed similar tensile strength between pure PLA and PLA with 50% WPC scaffolds. However, scaffolds with lower WPC concentrations displayed reduced tensile strength. Notably, all PLA/WPC scaffolds exhibited increased strain at break compared to pure PLA. Swelling capacity was highest in PLA with 25% WPC, approximately 130% higher than pure PLA. Scaffolds with higher WPC concentrations also showed increased swelling and degradation rates. Drug release was found to be prolonged with increasing WPC concentration. After seven days of incubation, cell viability significantly increased in PLA with 50% WPC scaffolds compared to pure PLA scaffolds. This innovative approach could pave the way for personalized wound care strategies, offering tailored treatments and targeted drug delivery. However, further studies are needed to optimize the properties of these scaffolds and validate their effectiveness in clinical settings.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-soluble antibacterial polyurethane based on cation mechanism and functionalized by chitosan and heparin azide. 基于阳离子机制并由壳聚糖和叠氮肝素功能化的非溶性抗菌聚氨酯。

Biomedical materials (Bristol, England) Pub Date : 2024-06-27 DOI: 10.1088/1748-605X/ad5a5c

Shuaishuai Li, Liang Zhang, Yicheng Liu, Enke Zhang, Xinyu Li, Zichi Chen, Zihan Yu, Haoyuan Zhou, Ying Li

{"title":"Non-soluble antibacterial polyurethane based on cation mechanism and functionalized by chitosan and heparin azide.","authors":"Shuaishuai Li, Liang Zhang, Yicheng Liu, Enke Zhang, Xinyu Li, Zichi Chen, Zihan Yu, Haoyuan Zhou, Ying Li","doi":"10.1088/1748-605X/ad5a5c","DOIUrl":"10.1088/1748-605X/ad5a5c","url":null,"abstract":"Nowadays, medical polyurethanes with favorable and durable antibacterial properties received more attention, because of avoiding repeated replacement of interventional materials and reducing patients' pain. In this thesis, non-soluble antibacterial polyurethane (NAPU) based on cation antibacterial mechanism was prepared by photo-grafting chitosan azide and heparin azide into polyurethane (PU). -NH3+of chitosan azide absorbed bacteria, inhibiting and breaking their mobility and structures. Heparin azide prevented cations from penetrating bacteria's membranes and inhibited their growth. The results showed that chitosan azide and heparin azide were successfully grafted into PU. The highest antibacterial rate was 92.07%, cytotoxicity grade ranging from 0-1 (RGR standard) and water contact angle exhibiting 60°, attributing to cation antibacterial effect and -OH existing. Tensile strength was up to 23.91 MPa and was suitable for using as medical materials. NAPU with long-lasting coating both possessed antibacterial properties and persistence, which can solve the problem of medical catheters' long-term using.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141433481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and effective method for smartphone-based detection of polyamines in oral cancer. 基于智能手机检测口腔癌多胺的简单有效方法。

Biomedical materials (Bristol, England) Pub Date : 2024-06-27 DOI: 10.1088/1748-605X/ad581a

Asmita Mojumdar, Unnikrishnan B S, Gopinath Packirisamy

{"title":"A simple and effective method for smartphone-based detection of polyamines in oral cancer.","authors":"Asmita Mojumdar, Unnikrishnan B S, Gopinath Packirisamy","doi":"10.1088/1748-605X/ad581a","DOIUrl":"10.1088/1748-605X/ad581a","url":null,"abstract":"Oral cancer accounts for 50%-70% of all cancer-related deaths in India and ranks sixth among the most frequent cancers globally. Roughly 90% of oral malignancies are histologically arise from squamous cells and are therefore called oral squamous cell carcinoma. Organic polycations known as biogenic polyamines, for example, putrescine (Put), spermidine (Spd), and spermine (Spm), are vital for cell proliferation, including gene expression control, regulation of endonuclease-mediated fragmentation of DNA, and DNA damage inhibition. Higher Spm and Spd levels have been identified as cancer biomarkers for detecting tumour development in various cancers. The current study utilises tannic acid, a polyphenolic compound, as a reducing and capping agent to fabricate AuNPs via a one-step microwave-assisted synthesis. The fabricated TA@AuNPs were utilised as a nanoprobe for colourimetric sensing of polyamines in PBS. When TA@AuNPs are added to the polyamine, the amine groups in polyamines interact with the phenolic groups of TA@AuNPs via hydrogen bonding or electrostatic interactions. These interactions cause the aggregation of TA@AuNPs, resulting in a red shift of the Surface Plasmon Resonance band of TA@AuNPs from 530 nm to 560 nm. The nanoprobe was found to be highly specific for Spm at low concentrations. TA@AuNPs were able to detect Spm successfully in artificial saliva samples. On recording the RGB values of the sensing process using a smartphone app, it was found that as the nanoparticles aggregated due to the presence of Spm, the intensity of theR-value decreased, indicating the aggregation of TA@AuNPs due to interaction with the polyamine.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genipin crosslinked quaternary ammonium chitosan hydrogels for wound dressings. Genipin 交联季铵壳聚糖水凝胶用于伤口敷料的潜力。

Biomedical materials (Bristol, England) Pub Date : 2024-06-26 DOI: 10.1088/1748-605X/ad525f

Ling Wang, Xiaoyue Ding, Jingyu Li, Man Li, Peng Ding, Wei Guo, Qiaoyun Wu, Yanfang Sun, Guohua Jiang, Oseweuba Valentine Okoro, Mahta Mirzaei, Amin Shavandi, Lihong Fan, Lei Nie

引用次数: 0