Biomedical materials (Bristol, England)最新文献_第9页

Dual-engineered small extracellular vesicles targeted delivery miR-126 for reducing age-related bone loss. 双工程小细胞外囊泡靶向递送miR-126减少与年龄相关的骨质流失。

Biomedical materials (Bristol, England) Pub Date : 2026-07-13 DOI: 10.1088/1748-605X/ae7ee3

Junming Tao, Ruiyu Du, Yi Zhang, Mengru Hong, Shaochen Duan, Datian Fu, Yue Chen, Chao Liang, Wei Jing

{"title":"Dual-engineered small extracellular vesicles targeted delivery miR-126 for reducing age-related bone loss.","authors":"Junming Tao, Ruiyu Du, Yi Zhang, Mengru Hong, Shaochen Duan, Datian Fu, Yue Chen, Chao Liang, Wei Jing","doi":"10.1088/1748-605X/ae7ee3","DOIUrl":"10.1088/1748-605X/ae7ee3","url":null,"abstract":"MiRNA based nucleic acid therapeutics have been extensively investigated for the treatment of age-related diseases. However, efficient delivery of miRNA and effective therapy for age-related bone loss remain major challenges. In this study, we devised an engineered small extracellular vesicle (sEV) platform to address the dysregulation of bone homeostasis in the elderly. Initially, miR-126 was encapsulated into sEV to generate miR-126 loaded small extracellular vesicles (m-sEV), whose capacity to enhance vascularized bone regeneration was validatedin vitroand in a mandibular defect model of aged rats. To further optimize systemic therapeutic efficacy, we functionalized m-sEV with a bone-targeting peptide:(DSS)6, thereby constructing bone-targeting engineered vesicles (Bm-sEV). Systemic administration of Bm-sEV enabled precise miR-126 targeted delivery to bone tissue, resulting in increased abundance of type H vessels in the femur, improved bone microarchitecture, and attenuation of age-related bone loss. Mechanistic analyses demonstrated that the angiogenesis-osteogenesis coupling effect was mediated by upregulation of endothelial Integrinβ3 (ITGB3), which subsequently activated the ITGB3/ERK2 signaling cascade. Notably, Bm-sEV also restored the profoundly impaired osteoclastic activity in aged femurs, thereby re-establishing skeletal homeostasis. Collectively, Our study provides a promising approach for the treatment of age-related bone loss by combining biological macromolecules such as (DSS)6and miR-126 with sEV.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemostatic and antibacterial performance of alginate/tannic acid-coated gauze for advanced wound care. 海藻酸/单宁酸包覆纱布的止血和抗菌性能。

Biomedical materials (Bristol, England) Pub Date : 2026-07-13 DOI: 10.1088/1748-605X/ae859b

Sun Min Hwang, Yu Jin Jeong, Jun Tae Kim, Byeong Joo Kim, Dongjin Lee, Su A Park, Oh Hyeong Kwon, Min Hee Kim, Won Ho Park

{"title":"Hemostatic and antibacterial performance of alginate/tannic acid-coated gauze for advanced wound care.","authors":"Sun Min Hwang, Yu Jin Jeong, Jun Tae Kim, Byeong Joo Kim, Dongjin Lee, Su A Park, Oh Hyeong Kwon, Min Hee Kim, Won Ho Park","doi":"10.1088/1748-605X/ae859b","DOIUrl":"10.1088/1748-605X/ae859b","url":null,"abstract":"Medical cotton gauze has traditionally been used as a local hemostatic material. However, there is a growing demand for innovative solutions that not only reduce blood loss but also prevent secondary infections. In the present study, a novel multifunctional hemostatic cotton gauze (designated as SGT-Ca@Gz) is developed by integrating sodium alginate, tannic acid, and calcium chloride (CaCl2) onto the gauze surface via a two-step dip-coating process. This technique provides the gauze with antibacterial and antioxidant properties, thereby effectively reducing both blood loss and the time needed for hemostasis. The ion cross-linking process employs a solution of CaCl2in a mixed water/ethanol solvent system to enhance the moisture stability of the polymer coating, ensure uniform surface cross-linking, increase the Ca2+content, and improve the mechanical properties of the gauze. As a result, the functional SGT-Ca@Gz exhibits enhanced antioxidant activity (89%) and demonstrates strong antibacterial activity againstE. coliandS. aureus. Inex vivoexperiments using a rat liver bleeding model, the SGT-Ca@Gz achieves a five-fold reduction in blood loss and a 2.8-fold decrease in hemostasis time compared to standard cotton gauze, thus highlighting its superior hemostatic performance. Consequently, this multifunctional hemostatic cotton gauze, with its enhanced antioxidant and antibacterial properties, is expected to have extensive applications in wound hemostasis.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148378268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In situgelling gelatin/modified starch hydrogel containing curcumin-loaded niosomes for wound healing application. 含有姜黄素的原位胶凝明胶/改性淀粉水凝胶用于伤口愈合。

Biomedical materials (Bristol, England) Pub Date : 2026-07-10 DOI: 10.1088/1748-605X/ae8231

Marziyeh Tahmasebi, Masuomeh Kaveh Zenjanab, Hamieh Goshtasbi, Marziyeh Fathi, Azam Safary, Hamid Erfan-Niya

{"title":"In situgelling gelatin/modified starch hydrogel containing curcumin-loaded niosomes for wound healing application.","authors":"Marziyeh Tahmasebi, Masuomeh Kaveh Zenjanab, Hamieh Goshtasbi, Marziyeh Fathi, Azam Safary, Hamid Erfan-Niya","doi":"10.1088/1748-605X/ae8231","DOIUrl":"10.1088/1748-605X/ae8231","url":null,"abstract":"Hydrogel dressings are one of the most appropriate agents for wound healing. Here, a novel, integrated hydrogel-based drug delivery system was developed for controlled curcumin (Cur) release for wound healing applications. To this purpose, a new hydrogel based on gelatin/ dialdehyde starch (DAS) was prepared using the Schiff-base reaction in the presence of curcumin (Cur)-loaded niosomes (HG/DAS/Nio/Cur). Cur was entrapped in Nio with an entrapment efficiency of 90%-95%. This system provides controlled release of Cur trapped in the hydrogel matrix and thus could improve its therapeutic ability. Dynamic light scattering indicated that Nio/Cur has particle sizes of 57.19 nm and a zeta potential of -8.21, respectively. The release rate from HG/DAS/Nio/Cur significantly increased, reaching 71% and 46% at pH = 5 and 7.4 after 72 h, respectively. The prepared hydrogels showed a high swelling ratio, and scanning electron microscopy revealed 3D porous structures (porosity around 87%-89%). Antimicrobial test showedE. coliinhibition effect but noS. aureusactivity due to the thick peptidoglycan barrier. MTT assays confirmed >90% viability of human umbilical vein endothelial cells. These results suggest the potential of HG/DAS/Nio/Cur as an advanced wound dressing system.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148320932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Youthful-state extracellular vesicles for targeted therapy of intervertebral disc degeneration: resolving inflammation and pyroptosis through NF-κB suppression and NLRP1 inactivation. 年轻状态细胞外囊泡靶向治疗椎间盘退变：通过抑制NF-κB和NLRP1失活来解决炎症和焦亡。

Biomedical materials (Bristol, England) Pub Date : 2026-07-10 DOI: 10.1088/1748-605X/ae7ebc

Zhiwei Liao, Kun Wang, Ming Lei, Bomansaan Herman, Shuai Li, Cao Yang, Huipeng Yin

{"title":"Youthful-state extracellular vesicles for targeted therapy of intervertebral disc degeneration: resolving inflammation and pyroptosis through NF-κB suppression and NLRP1 inactivation.","authors":"Zhiwei Liao, Kun Wang, Ming Lei, Bomansaan Herman, Shuai Li, Cao Yang, Huipeng Yin","doi":"10.1088/1748-605X/ae7ebc","DOIUrl":"10.1088/1748-605X/ae7ebc","url":null,"abstract":"Intervertebral disc degeneration pathogenesis involves chronic inflammation and cell death, highlighting the need for targeted therapeutic strategies. Extracellular vesicles (EVs) have emerged as promising bioactive materials for designing therapeutic approach. In this study, we demonstrate that youthful-state EVs (Y-EVs) outperform aged donor-derived EVs (O-EVs) in resolving inflammatory cascades within nucleus pulposus (NP) cells. EVs from young rats significantly suppressed TNF-α-induced inflammation in NP cells by reducing pro-inflammatory cytokine secretion, inhibiting extracellular matrix catabolism, and ameliorating rat disc degenerationin vivo. Mechanistically, CD55 enrichment in Y-EVs attenuated NF-κB pathway activation, thereby disrupting inflammatory transcriptional programs. CD55 knockdown abrogated the anti-inflammatory efficacy of Y-EV, confirming its functional necessity. Besides, we identified thioredoxin (TRX) as a critical suppressor of NLRP1 inflammasome activation via direct protein binding, which inhibited NP cell pyroptosis. Crucially, CD55 in Y-EVs facilitated TRX-mediated NLRP1 suppression, whereas O-EVs failed to upregulate TRX or suppress the NLRP1 inflammasome. This study highlights the age-dependent functional divergence of EV bioactivity and establishes CD55 as a key determinant of their therapeutic superiority. The TRX-NLRP1 interaction represents a novel target for disc degeneration intervention, positioning youthful-state EVs as an optimized bioactive material for disc regeneration strategies.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"21 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148426831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional modification of electrospun nanofibers for wound healing. 用于伤口愈合的静电纺纳米纤维的结构和功能改性。

Biomedical materials (Bristol, England) Pub Date : 2026-07-08 DOI: 10.1088/1748-605X/ae82a5

Sarah Fakher, Ryan Gilbert

{"title":"Structural and functional modification of electrospun nanofibers for wound healing.","authors":"Sarah Fakher, Ryan Gilbert","doi":"10.1088/1748-605X/ae82a5","DOIUrl":"10.1088/1748-605X/ae82a5","url":null,"abstract":"Effective wound repair requires a tightly regulated balance of cellular and molecular processes; however, conventional dressings primarily provide passive protection and often fail to actively guide tissue regeneration. Electrospun nanofibers have emerged as promising biomaterials due to their nanoscale architecture that mimics the extracellular matrix and their capacity for controlled therapeutic delivery. This review examines recent strategies designed to enhance the structural stability, mechanical performance, and biological functionality of electrospun nanofibers for advanced wound healing applications. Recent literature was evaluated to identify key material design approaches, supramolecular interactions, and post-fabrication modifications that improve scaffold performance and therapeutic outcomes. Native electrospun nanofibers are limited by instability in moist environments, insufficient mechanical resilience, and unpredictable release kinetics. Emerging solutions include structural modifications such as fiber alignment, multilayered architectures, and wettability tuning, as well as supramolecular strategies leveraging hydrogen bonding, electrostatic interactions, van der Waals forces, and hydrophilic-hydrophobic balance. Post-fabrication techniques, including plasma activation, layer-by-layer assembly, UV crosslinking, and hybrid composite formation, further enable the incorporation of antimicrobial, antioxidant, angiogenic, and immunomodulatory functionalities. These advances transform electrospun scaffolds from passive barriers into interactive platforms capable of regulating the wound microenvironment. By linking material design to biological outcomes, this review provides a framework for the rational development of next-generation multifunctional electrospun nanofibers with the potential to accelerate tissue repair and improve healing quality.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148334886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun PCL/PEG nanofibers incorporatingPlantago lanceolataextract and clove oil for dual-function wound dressings. 含有车前草提取物和丁香油的电纺PCL/PEG纳米纤维，用于双重功能伤口敷料。

Biomedical materials (Bristol, England) Pub Date : 2026-07-08 DOI: 10.1088/1748-605X/ae7ebd

Selin Nur Teke, Adile Yürük, İsmail Alper İşoğlu

{"title":"Electrospun PCL/PEG nanofibers incorporatingPlantago lanceolataextract and clove oil for dual-function wound dressings.","authors":"Selin Nur Teke, Adile Yürük, İsmail Alper İşoğlu","doi":"10.1088/1748-605X/ae7ebd","DOIUrl":"10.1088/1748-605X/ae7ebd","url":null,"abstract":"In this study, we developed electrospun polycaprolactone/polyethylene glycol (PCL/PEG) nanofibers loaded withPlantago lanceolata(P. lanceolata) extract and clove oil to evaluate their combined potential for wound healing and antibacterial activity. PCL/PEG nanofibers were electrospun and post-loaded withP. lanceolataextract at 5%, 10%, and 15% (w/v), together with 1% (v/v) clove oil. Scanning electron microscope analysis showed a uniform, bead-free nanofibrous structure, with fiber diameters ranging from 768 ± 140 nm to 892 ± 206 nm and pore sizes from 3.93 ± 0.97 µm to 6.20 ± 1.16 µm. The nanofibers exhibited swelling ratios between 103.23 ± 16.42% and 133.93 ± 40.45% within 1 h and showed gradual degradation ranging from 25.92 ± 2.84% to 57.76 ± 0.92% over 21 d, with cumulative extract release approaching a plateau by day 28 under the experimental conditions. The incorporation of plant extract and essential oil initially increased the water contact angle from 15.97 ± 1.07° to 48.61 ± 7.85°, indicating that the nanofiber surface remained hydrophilic; at higher extract contents, the nanofibers transitioned to a superhydrophilic state. Antibacterial activity was primarily governed by clove oil, yielding up to 85.16 ± 0.36% efficacy againstE. coli(E. coli) and 79.90 ± 0.29% againstS. aureus(S. aureus). WhileP. lanceolataextract alone showed limited antibacterial activity, its presence within clove oil-loaded nanofibers consistently enhanced antibacterial performance at the composite level.In vitroscratch assays demonstrated pronounced wound closure at later time points (72-96 h), particularly for nanofibers containing higher extract concentrations, confirming the dominant contribution ofP. lanceolatato the healing response. Overall, the electrospun PCL/PEG nanofibers represent a dual-function wound dressing, with clove oil contributing antibacterial protection andP. lanceolataextract supporting wound healing.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and fabrication of a 3D printed auxetic-electrospun scaffold for tendon structure modeling. 用于肌腱结构建模的3D打印辅助静电纺丝支架的设计与制造。

Biomedical materials (Bristol, England) Pub Date : 2026-07-08 DOI: 10.1088/1748-605X/ae7b9d

Giacomo Cortella, Alessandro Velletri, Erwin Pavel Lamparelli, Adamo Lancellotti, Mauro Di Stasi, Aurora De Acutis, Carmelo De Maria, Nicola Maffulli, Giovanna Della Porta, Giovanni Vozzi

{"title":"Design and fabrication of a 3D printed auxetic-electrospun scaffold for tendon structure modeling.","authors":"Giacomo Cortella, Alessandro Velletri, Erwin Pavel Lamparelli, Adamo Lancellotti, Mauro Di Stasi, Aurora De Acutis, Carmelo De Maria, Nicola Maffulli, Giovanna Della Porta, Giovanni Vozzi","doi":"10.1088/1748-605X/ae7b9d","DOIUrl":"10.1088/1748-605X/ae7b9d","url":null,"abstract":"Tendon tissue engineering requires scaffolds with an appropriate structure that can be cultured under cyclic strain in order to replicate both mechanical cues and biological commitments. This study presents a hybrid scaffold combining 3D-printed auxetic polycaprolactone (PCL) with electrospun gelatin-(3-Glycidyloxypropyl)trimethoxysilane (GPTMS) functionalized with collagen microfibers suitable for the purpose of cell culture under ciclyc strain andin vitromodeling of tenogenic events. The auxetic core was fabricated via pneumatic melt extrusion at 1000 mbar and printing speed of 200 mm min-1, achieving a re-entrant angle of 60.92 ± 2.1° and strut thickness of 350 ± 30 μm with high geometric fidelity. Mechanical characterization revealed that PCL scaffolds exhibited an elastic modulus of 51.97 ± 6.19 MPa and ultimate tensile strain of 6.75 ± 0.59%. Integration of electrospun gelatin-GPTMS without collagen increased the elastic modulus to 66.54 ± 7.16 MPa while reducing ultimate tensile strain to 4.14 ± 1.27%. Incorporation of 1 mg ml-1and 2 mg ml-1μCollaFibR™ significantly enhanced ultimate tensile strain to 21.70 ± 0.20% and 19.94 ± 0.20%, while decreasing elastic modulus to 47.65 ± 1.33 MPa and 39.83 ± 1.85 MPa. Field emission-scanning electron microscopy confirmed hierarchical architecture with collagen microfibers (1-5 μm diameter) randomly distributed throughout the electrospun gelatin matrix. Human tendon stem/progenitor cells seeded at 1 × 105cells cm-2maintained viability exceeding 85% at day 3 across all scaffold formulations, exhibiting elongated morphology and alignment along nanofiber axes. The described multimaterial platform successfully integrates auxetic mechanics with extracellular matrix-mimetic topographies, offering a biomimetic scaffold structure for subsequentin vitromodeling of tenogenic events.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148221220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An injectable biomimetic hydrogel with tethered TGFβ3 for treatment in a rat growth plate injury model. 带tgf - β3的可注射仿生水凝胶治疗大鼠生长板损伤模型。

Biomedical materials (Bristol, England) Pub Date : 2026-07-07 DOI: 10.1088/1748-605X/ae810c

Elizabeth Aisenbrey, Francisco Rodriguez-Fontan, Sarah A Schoonraad, Christopher B Erickson, Yangyi Yu, Stacey M Thomas, Kristine M Fischenich, Laurel Stefani, Karin A Payne, Stephanie J Bryant

{"title":"An injectable biomimetic hydrogel with tethered TGFβ3 for treatment in a rat growth plate injury model.","authors":"Elizabeth Aisenbrey, Francisco Rodriguez-Fontan, Sarah A Schoonraad, Christopher B Erickson, Yangyi Yu, Stacey M Thomas, Kristine M Fischenich, Laurel Stefani, Karin A Payne, Stephanie J Bryant","doi":"10.1088/1748-605X/ae810c","DOIUrl":"10.1088/1748-605X/ae810c","url":null,"abstract":"Injury to the growth plate, a cartilage tissue found at the ends of children's long bones that is responsible for bone elongation, can result in bony repair tissue (or bony bar). The bony bar connects bone on both sides of the growth plate, preventing normal bone elongation. This can lead to abnormal growth and if severe, complete growth arrest. While surgical resection of this bar is performed clinically, the interpositional materials used to replace it are susceptible to bony bar reformation. Here, we developed a novel interpositional material based on a biodegradable,in situphotopolymerizable, biomimetic hydrogel with tethered TGFβ3.In vitrostudies with rat mesenchymal stromal cells (MSCs) encapsulated in the hydrogel and cultured for 9 weeksin vitroshowed that tethered TGFβ3 led to a hyaline-like cartilage with collagen type II, while soluble TGFβ3 produced tissue characteristic of fibrocartilage, confirmed by collagen type I. Treatment of a rat growth plate injury with the TGFβ3-tethered hydrogel with or without MSCs decreased bony bar formation and showed evidence of cartilage-like tissue after four weeks, while addition of MSCs promoted limb lengthening. This hydrogel with tethered TGFβ3 offers an innovative treatment for growth plate injuries by acting as an interpositional material that resists bony bar formation and restores growth.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148310690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D-bioprinted head and neck cancer model for drug screening. 用于药物筛选的3d生物打印头颈癌模型。

Biomedical materials (Bristol, England) Pub Date : 2026-07-07 DOI: 10.1088/1748-605X/ae8069

Muhammad Rizwan, Xuan Mei, Ling Cai, Carlos Ezio Garciamendez-Mijares, Sushila Maharjan, Junmin Lee, Yu Shrike Zhang

{"title":"A 3D-bioprinted head and neck cancer model for drug screening.","authors":"Muhammad Rizwan, Xuan Mei, Ling Cai, Carlos Ezio Garciamendez-Mijares, Sushila Maharjan, Junmin Lee, Yu Shrike Zhang","doi":"10.1088/1748-605X/ae8069","DOIUrl":"10.1088/1748-605X/ae8069","url":null,"abstract":"The development of effective treatment strategies against head and neck cancer (HNC) poses a significant challenge due to the heterogeneity of HNC and the limitations of traditional two-dimensional (2D)in vitrocell cultures. Consequently, there is an urgent need for more advanced HNC models that allow for reliable drug screening. The recent advances in tissue engineering, including the use of digital light processing-based three-dimensional (3D) bioprinters, have enabled the precise fabrication of complex constructs that mimic native tissue environments, exhibiting potential for use in disease modeling and drug discovery. Here, we fabricated a bioprintedin vitroHNC model using the gelatin methacryloyl bioink encapsulating FaDu cells to analyze the drug screening against HNC. The bioprinted tissues showed gradually increased cell viability as well as stable morphology and phenotype expression. The cytotoxicity analyses suggested increased resistance to anti-cancer drugs for cells in 3D-bioprinted tissues than those cultured in 2D, possibly due to the presence of native cell-cell interactions and diffusion barriers. The engineered exosomes with anti-cancer drugs also revealed significantly increased resistance in cells cultured in 3D than in 2D. This study provides a proof-of-concept bioprinted HNC model mimicking some of the native functional and structural components, having the potential for screening novel anti-HNC treatments and paving the way for personalized therapeutic strategies for HNC patients in the future.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"21 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emulsion-based synthesis of polycaprolactone/bioactive glass 45S5 microparticles for bone regeneration applications. 用于骨再生应用的聚己内酯/生物活性玻璃45S5微颗粒的乳化合成。

Biomedical materials (Bristol, England) Pub Date : 2026-07-06 DOI: 10.1088/1748-605X/ae834e

Mantas Liudvinaitis, Dinas Tverijonas, Ieva Šimoliūnė, Milda Alksnė, Aleksej Žarkov, Povilas Daugėla, Mindaugas Pranskūnas, Egidijus Šimoliūnas

{"title":"Emulsion-based synthesis of polycaprolactone/bioactive glass 45S5 microparticles for bone regeneration applications.","authors":"Mantas Liudvinaitis, Dinas Tverijonas, Ieva Šimoliūnė, Milda Alksnė, Aleksej Žarkov, Povilas Daugėla, Mindaugas Pranskūnas, Egidijus Šimoliūnas","doi":"10.1088/1748-605X/ae834e","DOIUrl":"10.1088/1748-605X/ae834e","url":null,"abstract":"Polycaprolactone (PCL) and bioactive glass 45S5 (BG) are widely employed within bone tissue engineering due to their mutually complementary properties: PCL contributes structural integrity and biodegradability, whereas BG imparts osteoinductive properties to the composite. While patient-specific bulk scaffolds can restore structural defects, their fabrication requires individualized imaging, design, and manufacturing, increasing both time and cost. Hydrogels offer better conformity to irregular geometries but often lack sufficient mechanical stability and usually degrade rapidly. Microparticle-based systems represent a shape-adaptive alternative that can conform to complex defect geometries without customization, while maintaining structural integrity. In this study, PCL/BG microparticles (MPs) were fabricated using the emulsion solvent evaporation method and evaluatedin vitro. The microparticles supported adhesion and spreading, maintaining cell-cell and cell-particle interactions for over a month. Periosteum mesenchymal stem cells cultured on the MPs were responsive to adipogenic and osteogenic induction, as evidenced by increased expression of lineage-specific genes under both conditions. Overall, these findings suggest that PCL/BG microparticles are biocompatible and capable of guiding stem cell differentiation, supporting their potential use in bone regeneration strategies.","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0