Biomedical materials (Bristol, England)最新文献

{"title":"Response of a tenomodulin-positive subpopulation of human adipose-derived stem cells to decellularized tendon slices.","authors":"Xuan Li, Jing Cui, Liang-Ju Ning, Ruo-Nan Hu, Lei-Lei Zhao, Jia-Jiao Luo, Xin-Yue Xie, Yan-Jing Zhang, Jing-Cong Luo, Zheng-Yong Li, Ting-Wu Qin","doi":"10.1088/1748-605X/ada509","DOIUrl":"10.1088/1748-605X/ada509","url":null,"abstract":"<p><p>The selection of appropriate cell sources is vital for the regeneration and repair of tendons using stem cell-based approaches. Human adipose-derived stem cells (hADSCs) have emerged as a promising therapeutic strategy for tendon injuries. However, the heterogeneity of hADSCs can lead to inconsistent or suboptimal therapeutic outcomes. In this study, we isolated and identified a tenomodulin (TNMD)-positive subpopulation from hADSCs (TNMD⁺hADSCs) using flow cytometry and then assessed the cellular response of this subpopulation to decellularized tendon slices (DTSs), including cell proliferation, migration, and tenogenic differentiation, using the CCK-8 assay, transwell migration assay, and quantitative real-time polymerase chain reaction. Our findings revealed that TNMD⁺hADSCs maintained the general characteristics of stem cells and exhibited significantly higher expressions of tendon-related markers compared to hADSCs. Importantly, DTSs significantly enhanced the proliferation, migration, and tenogenic differentiation of TNMD⁺hADSCs. This study provides preliminary experimental evidence for the translational application of ADSCs for tendon regeneration and repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a multifunctional bio-probe based on lanthanides for UCL/MR/CT multimodal imaging<i>in vivo</i>.","authors":"Lijun Xiang, Chengying Wang, Yifu Mao, Yong Jiang, Zhifeng Hu, Yong Wang","doi":"10.1088/1748-605X/ada3d0","DOIUrl":"10.1088/1748-605X/ada3d0","url":null,"abstract":"<p><p>Multimodal bioimaging is beneficial for clinical diagnosis and research due to the provision of comprehensive diagnostic information. However, the design of multifunctional bio-probes aggregating multiple bioimaging functions is greatly challenging. In this study, a multifunctional bio-probe based on lanthanide-based nanomaterials Sr₂GdF₇: Yb³⁺/Er³⁺/Tm³⁺(abbreviated as SGF) was developed for<i>in vivo</i>multimodal imaging by co-adopting apropos lanthanides and tuning their molar ratio. The experimental results indicate that SGF incorporates multiple excellent properties, such as 10 nm small size, optimal red-NIR region emissions, strong paramagnetism, excellent x-ray absorption ability and high biological safety. More importantly, SGF successfully realized<i>in vivo</i>multimodal imaging of upconversion luminescence, magnetic resonance and x-ray computed tomography at the animal level. Thus, SGF is expected to become a multifunctional bio-probe for clinical research/diagnosis. This research would promote the application and transformation of lanthanide fluorides nanomaterials in the field of clinical diagnosis to a certain extent.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized cartilage tissue bioink formulation for osteochondral graft development.","authors":"Aleksandra A Golebiowska, Mingyang Tan, Anson Wk Ma, Syam P Nukavarapu","doi":"10.1088/1748-605X/ada59d","DOIUrl":"10.1088/1748-605X/ada59d","url":null,"abstract":"<p><p>Articular cartilage and osteochondral defect repair and regeneration presents significant challenges to the field of tissue engineering (TE). TE and regenerative medicine strategies utilizing natural and synthetic-based engineered scaffolds have shown potential for repair, however, they face limitations in replicating the intricate native microenvironment and structure to achieve optimal regenerative capacity and functional recovery. Herein, we report the development of a cartilage extracellular matrix (ECM) as a printable biomaterial for tissue regeneration. The biomaterial was prepared through decellularization and solubilization of articular cartilage. The effects of two different viscosity modifiers, xanthan gum and Laponite®, and the introduction of a secondary photo-crosslinkable component on the rheological behavior and stability were studied. dcECM-Laponite® bioink formulations demonstrated storage modulus (G') ranging from 750 to 4000 Pa, which is three orders of magnitude higher than that of the dcECM-XG bioink formulations. The rheological evaluation of the bioinks demonstrated the tunability of the bioinks in terms of their viscosity and degree of shear thinning, allowing the formulations to be readily extruded during 3D printing. Also, a spreadable ink composition was identified to form a uniform cartilage layer post-printing. The choice of viscosity modifier along with UV cross-linking warrants shape fidelity of the structure post-printing, as well as improvements in the storage and loss moduli. The modified ECM-based bioink also significantly improved the stability and allowed for prolonged and sustained release of loaded growth factors through the addition of Laponite®. The ECM-based bioink supported human bone-marrow derived stromal cell and chondrocyte viability and increased chondrogenic differentiation<i>in vitro</i>. By forming decellularized cartilage ECM biomaterials in a printable and stable bioink form, we develop a 'Cartilage Ink' that can support cartilaginous tissue formation by closely resembling the native cartilage ECM in structure and function.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanograin-enhanced surface-layer strengthening of 3D printed intervertebral cage induced by sandblasting.","authors":"Dongmei Yu, Suhua Wu, Taotao Zhao, Yongquan Zhang, Li Yao, Zeyang Zheng, Tao Li, Hao Wu, Ning Wang, Xiaokang Li, Chaozong Liu, Zheng Guo","doi":"10.1088/1748-605X/ad9fc5","DOIUrl":"10.1088/1748-605X/ad9fc5","url":null,"abstract":"<p><p>3D-printed customized titanium alloy (Ti6Al4V, TC4) as load-bearing prostheses and implants, such as intervertebral cages, have been widely used in clinical practice. Native biological inertia and inadequate bone in-growth of porous titanium alloy scaffolds hampered their clinical application efficiency and then extended the healing period. To improve the osseointegration capacity of 3D-printed intervertebral cages, sandblasting was selected to execute their surface treatment. On the one hand, sandblasting treatment can efficiently eliminate incomplete unmelted powder that adheres to struts in intervertebral cages during the manufacture of 3D printing, resulting in high surface area and low surface flatness induced by the rough surface could favor osseointegration. On the other hand, sandblasting can also induce ultrafine grains and nanograins in the near-surface layer that are conductive to mechanical strength enhancement. This can be verified by both microhardness and residual compressive stress reaching peak values (404.2 HV, 539.1 MPa) in the transverse section of its near-surface layer along the depth from the surface. This is attributed to the fact that more grain boundaries can impede dislocation movement. Sandblasting surfaces in intervertebral cages could favor osseointegration and in-growth, providing a foundation for sandblasting treatment of 3D-printed intervertebral cages in clinical applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical immunoassay for gastric cancer biomarker pepsinogen I detection based on PdAgPt/MoS₂.","authors":"Chunsheng He, Zhisong Qiu, Feng Jin, Lifang Weng, Libin Chen, Lijuan Wang, Sicong Jiang, Jin Shi","doi":"10.1088/1748-605X/ad9fc7","DOIUrl":"10.1088/1748-605X/ad9fc7","url":null,"abstract":"<p><p>This study presents a novel electrochemical immunosensor for the detection of pepsinogen I, a potential biomarker for gastric cancer, based on a unique PdAgPt/MoS₂nanocomposite. The key innovation lies in the synergistic combination of trimetallic PdAgPt nanoparticles with MoS₂nanoflowers, which has not been previously reported for pepsinogen I detection. This hybrid material demonstrates exceptional electron transfer properties and a significantly larger electroactive surface area compared to conventional materials. The optimized immunosensor exhibits superior performance metrics: a wide linear range of 0.5-200 ng ml^-1and an unprecedented low detection limit of 0.173 ng ml^-1, surpassing existing detection methods. The sensor shows remarkable selectivity with interfering substances exhibiting relative responses below 5%, excellent reproducibility (RSD 3.8%), and outstanding stability (95.6% retention after 30 d). Analysis of spiked serum samples resulted in recoveries ranging from 96.8% to 104.5%, demonstrating the sensor's practical applicability for early gastric cancer screening. This work represents a significant advancement in developing rapid, sensitive, and cost-effective diagnostic tools for gastric cancer surveillance.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable, cryopreservable mesenchymal stromal cell-loaded microbeads for pro-angiogenic therapy:<i>in vitro</i>proof-of-concept.","authors":"Francesco K Touani, Inès Hamouda, Nicolas Noiseux, Corinne Hoesli, Shant Der Sarkissian, Sophie Lerouge","doi":"10.1088/1748-605X/ad9af1","DOIUrl":"10.1088/1748-605X/ad9af1","url":null,"abstract":"<p><p>Despite their recognized potential for ischemic tissue repair, the clinical use of human mesenchymal stromal cells (hMSC) is limited by the poor viability of cells after injection and the variability of their paracrine function. In this study, we show how the choice of biomaterial scaffolds and the addition of cell preconditioning treatment can address these limitations and establish a proof-of-concept for cryopreservable hMSC-loaded microbeads. Injectable microbeads in chitosan, chitosan-gelatin, and alginate were produced using stirred emulsification to obtain a similar volume moment mean diameter (D[4,3] ∼ 500 µm). Cell viability was determined through live/dead assays, and vascular endothelial growth factor (VEGF) release was measured by ELISA. Proangiogenic function was studied by measuring the wound closure velocity of human umbilical vein endothelial cells (HUVEC) co-cultured with MSC-loaded microbeads. The effect of freeze-thawing on microbeads morphology, porosity, injectability and encapsulated MSC was also studied. hMSC-loaded chitosan-based microbeads were found to release 11-fold more VEGF than alginate microbeads (<i>p</i>< 0.0001) and chitosan-gelatin was chosen for further studies because it presented the best cell viability. Preconditioning with celastrol significantly enhanced the viability (1.12-fold) and VEGF release (1.40-fold) of MSC-loaded in chitosan-gelatin microbeads, as well as their proangiogenic paracrine function (1.2-fold;<i>p</i>< 0.05). In addition, preconditioning significantly enhanced the viability of hMSC after 1 and 3 d in low-serum medium after cryopreservation (<i>p</i>< 0.05). Cryopreserved hMSC-loaded microbeads maintained their mechanical properties, were easily injectable through a 23G needle, and kept their paracrine function, enhancing the proliferation and migration of scratched HUVEC. This study shows the advantage of chitosan as a scaffold material and concludes that chitosan-gelatin microbeads with celastrol-preconditioned cells form a promising off-the-shelf, cryopreservable allogenic MSC product.<i>In vivo</i>testing is required to confirm their potential in treating ischemic diseases or other clinical applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of synthesis strategies on morphology and antibacterial properties and photocatalytic activity of graphitic carbon nitride (g-C₃N₄).","authors":"Rojin Anbarteh, Maryam Aftabi-Khadar, Seyed Morteza Hosseini-Hosseinabad, Ashkan Seza, Soheil Rahmani Fard, Sara Minaeian","doi":"10.1088/1748-605X/ada23a","DOIUrl":"10.1088/1748-605X/ada23a","url":null,"abstract":"<p><p>Different morphologies of graphitic carbon nitride (g-C₃N₄), including bulk g-C₃N₄(B-CN), ultrathin nanosheet g-C₃N₄(N-CN), and porous g-C₃N₄(P-CN) were synthesized through a facile one-step approach. They were then employed as efficient photocatalysts under visible light to degrade methylene blue and deactivate<i>Staphylococcus aureus</i>(<i>S. aureus</i>) and<i>Escherichia coli</i>(<i>E. coli</i>) bacteria. The synthesized powders were characterized using various industry standard techniques and field emission scanning electron microscopy (SEM) analysis successfully represented the various morphologies of g-C₃N₄. Furthermore, the antibacterial activities of synthesized samples were examined, and the results revealed that B-CN, N-CN, and P-CN powders could eliminate around 64%, 82%, and 99% of<i>E. coli</i>under visible light irradiation and about 30%, 56, and 67% in dark conditions. On the other hand, the bacterial reduction rate of<i>S. aureus</i>was approximately 61%, 74%, and 99% for B-CN, N-CN, and P-CN powders under visible light irradiation and about 38%, 60%, and 77% in dark conditions. The SEM analysis revealed that P-CN caused<i>E. coli</i>and<i>S. aureus</i>bacteria to rupture, completely separating their internal contents from the cell membrane. g-C₃N₄photocatalytic antibacterial agents can be utilized as a unique potential solution for nosocomial infection management.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable nanocomposite hydrogels with co-delivery of oxygen and anticancer drugs for higher cell viability of healthy cells than cancer cells under normoxic and hypoxic conditions.","authors":"Nermin Seda Kehr","doi":"10.1088/1748-605X/ada240","DOIUrl":"10.1088/1748-605X/ada240","url":null,"abstract":"<p><p>Injectable nanocomposite hydrogels (NC hydrogels) have the potential to be used for minimally invasive local drug delivery. In particular, pH-sensitive injectable NC hydrogels can be used in cancer treatment to deliver high doses of anticancer drugs to the target site in cancer tissue without damaging healthy tissue. Recent studies have shown that in addition to stimuli-responsive delivery of anticancer drugs to cancer cells, oxygen delivery to the hypoxic environment of cancer tissue can lead to advanced effects, as hypoxia and an acidic pH are common characteristics of cancer tissue. However, few studies have investigated the effects of simultaneous administration of oxygen (O₂) and pH-dependent anticancer drugs via injectable NC hydrogels on the viability of healthy and cancer cells under normoxic and hypoxic conditions. In this context, we describe the synthesis of injectable NC hydrogels composed of pH-responsive nanomaterials carrying oxygen and anticancer drugs. Our system provides sustained O₂release and pH-responsive sustained release of anticancer drugs for 15 and 30 d, respectively. Moreover, O₂delivery and/or simultaneous delivery of O₂and anticancer drug resulted in higher cell survival of healthy fibroblast cells than malignant Colo-818 cells under hypoxic conditions (1% O₂) after 7 d of incubation.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid near and far field electrospinning of PVDF-TrFE/BaTiO₃scaffolds: morphology and osteoblast-like cell responses.","authors":"Larissa Mayra Silva Ribeiro, Krzysztof Berniak, Sunija Sukumaran, Rossano Gimenes, Urszula Stachewicz","doi":"10.1088/1748-605X/ada2cf","DOIUrl":"10.1088/1748-605X/ada2cf","url":null,"abstract":"<p><p>Scaffolds are of great interest in tissue engineering associated with regenerative medicine owing to their ability to mimic biological structures and provide support for new tissue formation. Several techniques are used to produce biological scaffolds; among them, far-field electrospinning (FFES) process is widely used due to its versatility in producing promising structures similar to native tissues owing to the electrospun nanofibers. On the other hand, near-field electrospinning (NFES) has been investigated due to the possibility of creating scaffolds with suitable architecture for their use in specific biological tissues. Thus, we investigated the potential of the electrospun scaffolds prepared using both techniques FFES and NFES, with tailored properties to mimic bone tissue native matrix and enhance the cell response. We produced scaffolds with the piezoelectric poly(vinylidene fluoride-trifluoroethylene) combined with BaTiO₃nanoparticles. Hence, the properties of both scaffolds were evaluated in terms of crystallinity and cell behavior, such as adhesion, proliferation and cell viability. Microstructure properties showed good thermal stability, similar crystallinity (∼65%) and a<i>β</i>-phase content of ∼40% for both scaffolds. For biological tests, MG-63 osteoblast-like cells were used, and for NFES scaffolds, we noted that the proliferation and cell alignment followed the fiber pattern and created a bridge between adjacent fibers. In contrast, cells spread and proliferated randomly on the surface of the FFES scaffold. Despite the differences in cell behavior, both scaffolds showed good biocompatibility in terms of functional scaffolds with suitable characteristics for use in the area of tissue regeneration.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of antibacterial silicone rubber catheter modified with the biomass carbon dots and its properties.","authors":"Jing Liu, Ziyang Shao, Liyuan Liu, Qinke Zhao, Yongzhen Yang, Mei Niu, Li Zhang, Baoxia Xue","doi":"10.1088/1748-605X/ad9f03","DOIUrl":"10.1088/1748-605X/ad9f03","url":null,"abstract":"<p><p>To endow silicone rubber (SR) catheter with antibacterial property, the SR catheter was modified with a new kind of biomass carbon dots (CDs) by the bulk modification to obtabin the SR/CDs catheter. The antimicrobial behavior and biocompatibility of SR/CDs catheter were analyzed by plate counting method, cytotoxicity test and<i>in vivo</i>animal experiments. The results showed that, SR/CDs catheter possessed antimicrobial properties, and the minimum inhibitory concentration of SR/CDs catheter was 20 mg ml^-1against<i>Escherichia coli</i>and<i>Staphylococcus aureus</i>. The antimicrobial mechanism of SR/CDs was further investigated, and it was found that the SR/CDs induced the production of reactive oxygen species in bacterial cells by disrupting the bacterial membrane through adsorption. In addition,<i>in vivo</i>experiments have shown that SR/CDs catheter owns good biosafety profile and reduces the risk of catheter-associated urinary tract infections by modulating inflammatory factors. Meanwhile, SR/CDs catheter can be produced in a simple production process using an extruder, which is expected to be used as a novelty type of catheter in the clinic.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}