Development of a polydopamine-graphene nanocomposite electrochemical aptasensor for sensitive detection of lung cancer biomarker VEGF₁₆₅.

Biomedical materials (Bristol, England) Pub Date : 2025-09-09 DOI:10.1088/1748-605X/adffd2

Yunguo Liao, Hong Hu, Ziyu Tang, Dan Deng, Chao Li

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Abstract

This study presents a novel electrochemical aptasensor utilizing polydopamine-graphene (PDA-G) nanocomposite for VEGF₁₆₅detection. The PDA-G nanocomposite was synthesized through a one-step self-polymerization process and characterized by SEM, XPS, and FTIR, confirming successful PDA coating on graphene sheets with 22.3% C-N bond incorporation. Electrochemical characterization confirmed stepwise increases in charge transfer resistance (Rct), and quantitative detection was based on ΔRct (the change in Rct before vs. after VEGF₁₆₅binding). Under optimized conditions (pH 7.4, 37 °C, 30 min incubation), the sensor demonstrated dual-linear response regions with high sensitivity. Analysis of clinical samples showed minimal matrix effects with a 1:4 dilution ratio, and endogenous VEGF₁₆₅levels in healthy donor serum were determined to be 28.4 ± 3.2 pg ml^-1. The method's practicality was validated through comprehensive interference studies, with common serum proteins generating relative responses below 5.2% compared to the target.

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用于肺癌生物标志物VEGF165敏感检测的聚多巴胺-石墨烯纳米复合电化学感应传感器的研制

本研究提出了一种利用聚多巴胺-石墨烯（PDA-G）纳米复合材料检测VEGF165的新型电化学感应传感器。通过一步自聚合工艺合成了PDA- g纳米复合材料，并通过SEM， XPS和FTIR对其进行了表征，证实了PDA在石墨烯片上的成功涂层，其C-N键的掺杂率为22.3%。电化学表征证实了电荷转移电阻（Rct）逐步增加，定量检测基于ΔRct （Rct与VEGF165结合前后的变化）。在优化条件下（pH 7.4, 37°C， 30分钟孵育），传感器具有高灵敏度的双线性响应区。临床样品分析显示基质效应最小，稀释比例为1:4，健康供者血清中内源性VEGF165水平为28.4±3.2 pg/mL。通过综合干扰试验验证了该方法的实用性，常见血清蛋白相对于靶蛋白的相对应答率低于5.2%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedical materials (Bristol, England)

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