Biomedical materials (Bristol, England)最新文献

{"title":"Gold nanocages co-assembled with<i>Spinacia oleracea</i>extract combined photothermal/photodynamic therapy in 4T1 breast cancer cell line.","authors":"Dheeraj Dehariya, Anindita Tarafdar, Monika Pebam, Sri Amruthaa Sankaranarayanan, Sajmina Khatun, Aravind Kumar Rengan","doi":"10.1088/1748-605X/ada83e","DOIUrl":"10.1088/1748-605X/ada83e","url":null,"abstract":"<p><p>Photothermal therapy (PTT) and photodynamic therapy (PDT) have been emerging as potential alternatives to conventional cancer treatment modalities. Gold nanoparticles, owing to their surface plasmon resonance properties, have been promising in cancer phototherapies, and extracts from potent medicinal plants are commonly employed for the green synthesis of various nanoparticles. Some researchers have used photosensitizers like chlorophyll to promote reactive oxygen species generation. In this research, the photothermal ability of gold and the photon-absorbing capability of chlorophyll derived from<i>Spinacia oleracea</i>(<i>S. oleracea</i>) are combined to achieve the optimum results. Herein, we have synthesized the gold nanocages(AuNCs) co-assembled with<i>S. oleracea</i>extract (SPAuNCs; 70 ± 10 nm) to be employed as a PTT and PDT agent to treat triple-negative breast cancer. This study found that SPAuNCs are promising PTT and PDT agents against breast cancer cell line.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atomic-layer-deposition application for antibacterial coating of biomedical materials: surgical sutures.","authors":"Ilmutdin M Abdulagatov, Visampasha Yu Khanaliev, Razin M Ragimov, Abai M Maksumova, Мagomed А Khamidov, Naida M Abdullaeva, Naida R Mollaeva","doi":"10.1088/1748-605X/ada841","DOIUrl":"10.1088/1748-605X/ada841","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Suture-associated surgical site infection (SSI) causes bacterial pathogens to colonize on the suture surface that are highly resistant to antibiotic treatment. Conventional suture materials used in surgical practice are causing complications such as infection and chronic inflammation. Surgical suture materials with antibacterial coatings are widely used in surgical practice. However, all the widely used antibacterial agents are not permanent (limited lasting) due to their instability and release depending on environmental conditions (pH or temperature, for example). Therefore, more long-lasting (low-dose) and effective antibacterial function materials are required. In the present work, we proposed a new material and method of antibacterial coating the surgical sutures based on the atomic layer deposition (ALD) technique to enhance its antibacterial activity for treatment of the SSI. We have proposed applying a vanadium-doped TiO&lt;sub&gt;2&lt;/sub&gt;nanofilm (hybrid nanomaterial, TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;) with 27.5 nm thickness to enhance the antibacterial property of surgical sutures using the ALD technique. We have illustrated that a base coating of Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt;(seed layer) applied to the suture surface, which directly contacts the polypropylene (PP) suture, improves the adhesion of the deposited antibacterial material TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;. This provides a long-lasting antibacterial effect on the suture (a prolonged antibacterial effect of the coating material), i.e. increases the stability of the deposition (stable in water, air, in the human body, in different pH mediums, and at temperatures up to 70 °C). The sutures did not deteriorate after several wash cycles with sterilizing solvents. Also, the antibacterial agent (TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;) is nontoxic. The concentration of vanadium in the film is below the toxicity limits due to the low diffusivity of vanadium and high adhesion with the base coating material (Al&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;3&lt;/sub&gt;). Sutures coated with V-doped TiO&lt;sub&gt;2&lt;/sub&gt;were characterized using scanning electron microscopy images, and elemental analysis was performed using energy dispersive spectroscopy Spectroscopy. The antibacterial activity of TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;coated sutures against two types of microorganisms,&lt;i&gt;E. coli&lt;/i&gt;and Proteus vulgaris (&lt;i&gt;Pr. Vulgaris&lt;/i&gt;) was compared to that of noncoated sutures. The quantitative assessment of antibacterial activity of suture materials with and without ALD nanocoating TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;against&lt;i&gt;E. coli&lt;/i&gt;and&lt;i&gt;Pr. Vulgaris&lt;/i&gt;has been performed. No growth of bacteria around the suture material with antibacterial TiVO&lt;i&gt;&lt;sub&gt;x&lt;/sub&gt;&lt;/i&gt;ALD nanocoating throughout the entire observation period of 48 and 72 h was observed. However, after 48 h, the concentration of bacteria of the&lt;i&gt;E. Coli&lt;/i&gt;around the suture material without ALD TiVO&lt;i&gt;x&lt;/i&gt;nanocoating on nutrient agar was 5.5 ± 0.3 Log CFU cm&lt;sup&gt;-3&lt;/sup&gt;, and after 72 h it was 8.0 ± 0.5 Log CFU cm&lt;sup&gt;-3","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and development of vaginal wall mimicking poly(<i>ϵ</i>-caprolactone) based nanofibrous prosthetic mesh for pelvic organ prolapse: evaluation of biocompatibility and antibacterial ability.","authors":"Preethi Arul Murugan, Jayesh Bellare","doi":"10.1088/1748-605X/ada2d0","DOIUrl":"10.1088/1748-605X/ada2d0","url":null,"abstract":"<p><p>Mechanical non-conformance of conventionally used transvaginal non-degradable meshes has led to complications such as organ perforation, dyspareunia caused by mesh stiffness and stress shielding. In this study, we have solved the dire need to mimic the mechanical properties of the vaginal wall by designing and developing a soft and elastic mesh made of polycaprolactone (PCL), citric acid modified polyethylene glycol (PEGC) and zinc oxide (ZnO) prepared through electrospinning and tested<i>in vitro</i>and<i>in vivo</i>. The mesh containing 90:10:0.1 of PCL, PEGC and ZnO (PEGC-15 0.1ZnO mesh) conforms to the mechanical properties of the vaginal wall of the pelvic floor, has a burst strength of ∼35 N even after gamma-sterilization and 28 d of degradation in<i>in vitro</i>.<i>In vitro</i>studies using adipose-derived stem cells revealed that the PCL-PEGC-15 0.1ZnO meshes were biocompatible and supported higher collagen production than commercial mesh.<i>An in vitro</i>bacterial adhesion study showed a 2-log reduction compared to commercially available mesh for prolapse treatment. Initial biocompatibility assessment in a rabbit model also showed that the PCL-PEGC-15 0.1ZnO mesh is biocompatible and supports fibrosis throughout the mesh. The softness and flexibility of the PCL-PEGC-15 0.1ZnO mesh based on<i>in vitro</i>trials and initial<i>in vivo</i>trials show that the mesh has a potential clinical impact for pelvic floor repair treatment.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i>evaluation of bioabsorbable poly(lactic acid) (PLA) and poly-4-hydroxybutyrate (P4HB) warp-knitted spacer fabric scaffolds for osteogenic differentiation.","authors":"Flavia Caronna, Skander Limem, Ly Dang Khoa Do, William Ronan, Eimear B Dolan","doi":"10.1088/1748-605X/ada85d","DOIUrl":"10.1088/1748-605X/ada85d","url":null,"abstract":"<p><p>Bioabsorbable textile scaffolds are promising for bone tissue engineering applications. Their tuneable, porous, fibre-based architecture resembles that of native extracellular matrix, and they can sustain tissue growth while being gradually absorbed in the body. In this work, immortalized mouse calvaria preosteoblast MC3T3-E1 cells were cultured<i>in vitro</i>on two warp-knitted bioabsorbable spacer fabric scaffolds made of poly(lactic acid) (PLA) and poly-4-hydroxybutyrate (P4HB), to investigate their osteogenic properties. Scaffold structure and yarn properties were characterized after manufacturing. Cells were seeded on the two scaffolds and treated with osteogenic media for up to 35 days. Both scaffolds supported similar cell growth patterns, featuring a higher cell density on multifilament yarns, which could be beneficial to drive cell proliferation or related phenomena in localized area of the construct. The increase in alkaline phosphatase activity and the calcium deposition observed on some PLA and P4HB scaffolds after 28 and 35 days of culture, confirm their potential to support MC3T3-E1 cells differentiation, however inconsistent mineralization was observed on the scaffolds. Due to their structural and morphological features, ability to support cell attachment and growth, and their limited osteogenic potential, these PLA and P4HB bioabsorbable textile scaffolds are recommended for further investigation for bone tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing osteogenic properties with gelatin/chitosan hydrogel encapsulating lithium-coated titanium oxide hollow sphere particles loaded with quercetin.","authors":"Qingjie Wang, Liang Zhang","doi":"10.1088/1748-605X/adae6e","DOIUrl":"https://doi.org/10.1088/1748-605X/adae6e","url":null,"abstract":"<p><p>Metallic oxides especially lithium and titanium oxides are well known for their osteogenic properties. When combined in the right proportions, metallic oxides can have an even greater impact. However, releasing ions from oxides can lead to oxidative stress, which is harmful to cell growth. By reducing oxidative stress, we can enhance these ions' therapeutic and bone-forming properties. In our study, we have developed a novel combination of titanium oxide coated with lithium oxide to release ions simultaneously. We engineered hollow sphere titanium oxide particles to carry Quercetin (QC), a natural antioxidant. These particles were then incorporated into a gelatin/chitosan-based hydrogel, which was further functionalized with carbon nanotubes (CNTs) which induced conductivity and improved mechanical properties. In drug release experiments, we found that QC was released steadily from the hydrogel, in contrast to a control group where the drug was simply mixed in with hydrogel indicating the significance of a secondary carrier. Additionally, our cytotoxicity tests demonstrated the importance of delivering QC alongside lithium and titanium ions, as this combination reduced toxicity and enhanced bone-forming activity. Finally, our study showed that the hydrogel containing drug-loaded hollow sphere particles was able to promote bone formation, as evidenced by osteogenic differentiation studies. This innovative approach holds promise for improving bone regeneration therapies in the future.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atelocollagen-based hydrogel loaded with<i>Cotinus coggygria</i>extract for treatment of type 2 diabetic wounds.","authors":"Candan Yilmaz Ozdogan, Halime Kenar, Huseyin Uzuner, Aynur Karadenizli","doi":"10.1088/1748-605X/ada7b5","DOIUrl":"10.1088/1748-605X/ada7b5","url":null,"abstract":"<p><p>Diabetes, a chronic metabolic disease, causes complications such as chronic wounds, which are difficult to cure. New treatments have been investigated to accelerate wound healing. In this study, a novel wound dressing from fibroblast-laden atelocollagen-based hydrogel with<i>Cotinus coggygria</i>extract was developed for diabetic wound healing. The antimicrobial activity of<i>C. coggygria</i>hexane (H), dichloromethane (DCM), dichloromethane:methanol (DCM-M), methanol (M), distilled water (DW) and traditional (T) extracts against<i>Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis</i>and<i>Candida albicans</i>, as well as their cytotoxic effects on fibroblasts were determined. While fibroblast growth was significantly (<i>p</i>< 0.05) promoted with DCM (121.41 ± 1.04%), M (109.40 ± 5.89%) and DW (121.83 ± 6.37%) extracts at their lowest concentrations, 2000 μg ml^-1DCM and 7.8 μg ml^-1T extracts had both non-cytotoxic and antifungal effects. An atelocollagen-based hydrogel was produced by thermal crosslinking, and its pore size (38.75 ± 7.67 μm), water content (96.63 ± 0.24%) and swelling ratio (27.21 ± 4.08%) were found to be suitable for wound dressings. A significant increase in the deoxyribonucleic acid amount (28.27 ± 1.41%) was observed in the plain hydrogel loaded with fibroblasts after 9 d of incubation, and the hydrogel had an extensively interconnected cellular network. The hydrogels containing DW and T extracts were applied to wounds generated in an<i>in vitro</i>3D type-2-diabetic human skin model. Although the incubation period was not sufficient for closure of the wounds in either of the treatments, the hydrogel with T extract stimulated more fibroblast migration. In the fibroblast-laden version of the hydrogel with T extract, no wound closure was observed but more keratinocytes migrated to the wound region. These positive outcomes underline the potential of the developed wound dressing as a powerful alternative to improve diabetic wound healing in clinical practice.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macroporous coating of silver-doped hydroxyapatite/silica nanocomposite on dental implants by EDTA intermediate to improve osteogenesis, antibacterial, and corrosion behavior.","authors":"Maryam Farmani, Seyede Zohreh Mirahmadi-Zare, Elahe Masaeli, Farideh Tabatabaei, Arezou Baharlou Houreh","doi":"10.1088/1748-605X/ad971d","DOIUrl":"10.1088/1748-605X/ad971d","url":null,"abstract":"<p><p>Coating a titanium (Ti) implant with hydroxyapatite (HA) increases its bioactivity and biocompatibility. However, implant-related infections and biological corrosion have restricted the success of implant. To address these issues, a modified HA nanocomposite (HA/silica-EDTA-AgNPs nanocomposite) was proposed to take advantage of the sustained release of silver nanoparticles (AgNPs) and silicate ions through the silica-EDTA chelating network. As a result, a uniform layer of nanocomposite, compared to HA as the gold standard, was formed on Ti implants without fracture and with a high level of adhesion, using plasma electrolytic oxidation (PEO). Bioactivity assessment evidenced a shift in the surface phase of the Ti implant to generation of beta-tricalcium phosphate, a more bioresorbable material than HA. Metabolic activity assessments using human dental pulp stem cells revealed that Ti surfaces modified by the new nanocomposite are superior to bare and HA-modified Ti surfaces for cell attachment and proliferation<i>in vitro</i>. In addition, it successfully inhibited bacterial growth and induced osteogenesis on the implant surface. Finally, potentiodynamic polarization behavior of Ti implants before and after coating confirmed that a thick oxide interface layer on the modified Ti surface acts as an electrical barrier and protects the substrate layer from corrosion. Therefore, the HA/silica-EDTA/Ag nanocomposite presented here, compared to HA, can better coat Ti dental implants due to its good biocompatibility and osteoinductive activity, along with improved biological stability.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcytosis: an effective mechanism to enhance nanoparticle extravasation and infiltration through biological barriers.","authors":"Qianyi Zhang, Jiamian Wang, Zhiyang Chen, Hao Qin, Qichen Zhang, Bo Tian, Xilei Li","doi":"10.1088/1748-605X/ada85e","DOIUrl":"10.1088/1748-605X/ada85e","url":null,"abstract":"<p><p>Nanoparticles (NPs)¹have been explored as drugs carriers for treating tumors and central nervous system (CNS)²diseases and for oral administration. However, they lack satisfactory clinical efficacy due to poor extravasation and infiltration through biological barriers to target tissues. Most clinical antitumor NPs have been designed based on enhanced permeability and retention effects which are insufficient and heterogeneous in human tumors. The tight junctions³3TJs: tight junctionsof the blood-brain barrier⁴4BBB: blood-brain barrierand the small intestinal epithelium severely impede NPs from being transported into the CNS and blood circulation, respectively. By contrast, transcytosis enables NPs to bypass these physiological barriers and enhances their infiltration into target tissues by active transport. Here, we systematically review the mechanisms and putative application of NP transcytosis for targeting tumor and CNS tissues, explore oral NP administration, and propose future research directions in the field of NP transcytosis.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142959708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of physicochemical property changes in 3D-printed biodegradable medical devices under simulated oral physiological conditions.","authors":"Eungtae Lee, Yeonguk Seong, Jihee Jeong, Yongbin Ji, Joonho Eom, Changwon Park, Jinhyun Kim, Sangbae Park, Jong Hoon Chung","doi":"10.1088/1748-605X/ada85f","DOIUrl":"https://doi.org/10.1088/1748-605X/ada85f","url":null,"abstract":"<p><p>Biodegradable medical devices undergo degradation following implantation, potentially leading to clinical failure. Consequently, it is necessary to assess the change in their properties post-implantation. However, a standardized method for the precise evaluation of the changes in their physicochemical properties is currently lacking. In this study, we aimed to establish precisely simulated oral physiological conditions (SOPCs) and investigate the physicochemical property changes to predict the performance alterations of biodegradable dental barrier membranes (BDBMs) following human implantation. We investigated changes in physicochemical properties of BDBM after exposure to SOPC for 24 weeks. When BDBM was exposed to SOPC for 24 weeks, there was a significant decrease in mass (-1.37%), molecular weight (-19.54%) and tensile load (-72.84%). Among the physicochemical properties, molecular weight decreased similarly after 24 weeks of implantation in rats (-15.78%) and after 24 weeks of exposure to SOPC (-19.54%). Changes in the physicochemical properties of BDBM in simulated<i>in vitro</i>oral conditions and in the<i>in vivo</i>environment were similar. Overall, the evaluation of physicochemical property changes after exposing BDBM to the proposed SOPC demonstrates novelty in its ability to accurately predict performance changes post-implantation. This approach may provide significant insights not only for the development of BDBM but also for various types of biodegradable medical devices.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A xenogenic-free culture medium for cell micro-patterning systems as cell-instructive biomaterials for potential clinical applications.","authors":"Hui Che, Melanie L Hart, Jasmin C Lauer, Mischa Selig, Marita Voelker, Bodo Kurz, Bernd Rolauffs","doi":"10.1088/1748-605X/ada335","DOIUrl":"10.1088/1748-605X/ada335","url":null,"abstract":"<p><p>Cell micro-patterning controls cell fate and function and has potential for generating therapeutically usable mesenchymal stromal cell (MSC) populations with precise functions. However, to date, the micro-patterning of human cells in a translational context has been impossible because only ruminant media supplements, e.g. fetal bovine serum (FBS), are established for use with micro-patterns (MPs). Thus, there are currently no good manufacturing practice (GMP)-compliant media available for MPs. This study tested a xenogenic-free human plasma and platelet lysate (hP + PL) medium supplement to determine its compatibility with MPs. Unfiltered hP + PL medium resulted in significant protein deposition, creating a 'carpet-like' layer that rendered MPs ineffective. Filtration (3×/5×) eliminated this effect. Importantly, quantitative comparison using droplet digital PCR revealed that human MSCs in all media types exhibited similar profiles with strong myogenic Calponin 1/Transgelin 2 (TAGLN2) and weaker osteogenic alkaline phosphatase/Runt-related transcription factor 2 marker expression, and much weaker adipogenic (lipoprotein lipase/peroxisome proliferator-activated receptor gamma) and chondrogenic (collagen type II/aggrecan) expression, with profiles being dominated by myogenic markers. Within these similar profiles, an even stronger induction of the myogenic marker TAGLN2 by all hP + PL- compared to FBS-containing media. Overall, this suggested that FBS can be replaced with hP + PL without altering differentiation profiles. However, assessing individual MSC responses to various MP types with defined categories revealed that unfiltered hP + PL medium was unusable. Importantly, FBS- and 3× filtered hP + PL media were comparable in each differentiation category. Summarized, this study recommends 3× filtered hP + PL as a xenogenic-free and potentially GMP-compliant alternative to FBS as a culture medium supplement for micro-patterning cell populations in both basic and translational research that will ensure consistent and reliable MSC micro-patterning for therapeutic use.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}