Biomedical materials (Bristol, England)最新文献

{"title":"PLGA nanoparticles encapsulating TSHR-A and rapamycin enhance the induction of dendritic cell-specific immune tolerance in mice with Graves' disease.","authors":"Kun Chen, Yu Yang, Yang Wu, Wen Cao, Yijing Zhao, Su Wang, Kun Wang","doi":"10.1088/1748-605X/adbaa3","DOIUrl":"10.1088/1748-605X/adbaa3","url":null,"abstract":"<p><p>Dendritic cells (DCs) are the most potent antigen-presenting cells with multifaceted functions in controlling immune activation and tolerance. Graves' disease, particularly Graves' ophthalmopathy, is recognized as a refractory autoimmune thyroid disease. Therefore, DC-targeted therapies aimed at inducing specific immune tolerance are important for the treatment of Graves' disease. Therefore, we utilized polylactic acid glycolic acid polymer (PLGA) polymer nanoparticles (NPs) encapsulating Graves' disease auto-antigen thyrotropin receptor A (TSHR-A) peptide and the immune tolerance inducer rapamycin (Rapa) to synthesize drug-loaded NPs (NP (TSHR-A + Rapa)). We first characterized the synthesized nanodrugs using transmission electron microscopy and dynamic light scattering techniques and tested the uptake capacity of DCs for NPs after co-culturing the NPs with DCs. And the safe concentration of NPs to DCs was detected using Cell counting kit-8 (CCK-8) assay. Subsequently, we tested the targeting and safety of the NPs in mice. And the effects of NPs on the proportion and proliferation of DCs and regulatory T (Treg) cells were examined<i>in vivo</i>and<i>in vitro</i>using flow cytometry and 5-ethynyl-2'-deoxyuridine (EdU) method, respectively. Enzyme linked immunosorbent assay (ELISA) assays were used to detect the effect of NPs on cytokine release from DCs. Finally, we tested the preventive and therapeutic effects of the synthesized NPs on disease models. Our results showed that the synthesized NPs were well taken up by DCs<i>in vitro</i>, while<i>in vivo</i>they were mainly targeted to the spleen of mice. The NPs were able to relatively inhibit the maturation of DCs<i>in vivo</i>and<i>in vitro</i>, while affecting the release of relevant cellular functional factors from DCs, and the NPs also promoted the proportion and proliferation of Treg cells<i>in vivo</i>and<i>in vitro</i>. In addition, the synthesized NPs were able to prevent and improve the mouse disease model well without toxic side effects on mouse organs and other physiological indicators. Therefore, the synthesis of NP (TSHR-A + Rapa) NPs using PLGA encapsulated TSHR-A and rapamycin could be used as targeting DCs to alter immune tolerance and as a new potential approach for the treatment of Graves' disease.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing controlled degradation, bioactivity, and mechanical behavior in sol-gel synthesized aluminum titanate biomaterials.","authors":"Shanmugapriya B, Shailajha S, Sakthi Muthulakshmi S","doi":"10.1088/1748-605X/adbb46","DOIUrl":"10.1088/1748-605X/adbb46","url":null,"abstract":"<p><p>Orthopaedic applications require materials that balance mechanical strength, biocompatibility, and controlled degradation, particularly for bone regeneration and load-bearing purposes. This study investigates the effects of varying weight percentages of Al₂O₃and TiO₂(25:75, 50:50, and 75:25) on the characteristics of Al₂TiO₅biomaterials synthesized via the sol-gel method. Structural and chemical characterizations, including XRD and FTIR, confirmed the successful synthesis of phase-pure Al₂TiO₅, highlighting functional groups such as Al-O and Ti-O. Among the tested compositions, the 50:50 ratio exhibited the strongest antibacterial efficacy against<i>S. aureus</i>and<i>E. coli</i>, comparable to a commercial antibiotic, while also promoting hydroxyapatite (HAp) deposition in simulated body fluid (SBF). Additionally, cytotoxicity assessments using the L929 murine fibroblast cell line revealed that the 50:50 composition had the lowest toxicity. All formulations demonstrated controlled degradation, minimizing pH fluctuations and enhancing bioactivation and biocompatibility. Zeta potential analysis indicated that the 50:50 composition exhibited the most negative values over time, suggesting strong surface interactions with SBF and a favorable environment for HAp nucleation. Furthermore, the compressive strength of all formulations (71-74 MPa) was sufficient for load-bearing applications. These findings suggest that optimizing the 50:50 weight ratio enhances bioactivity, mechanical stability, and biocompatibility, making it a promising candidate for orthopedic and bone tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and application of chitosan nanoparticles for bone tissue regeneration.","authors":"Jian Li, Chun Fan, Baodong Zhao, Ye Liang","doi":"10.1088/1748-605X/adbb45","DOIUrl":"10.1088/1748-605X/adbb45","url":null,"abstract":"<p><p>Bone defects, resulting from trauma, tumor removal, infection, or congenital anomalies, are increasingly prevalent in clinical practice. Progress in bone tissue engineering has significantly advanced bone regeneration techniques. Chitosan-based nanoparticles (ChNPs) have emerged as a promising drug delivery system due to their inherent ability to enhance bone regeneration. These nanoparticles can extend the activity of osteogenic factors while ensuring their controlled release. Common synthesis methods for ChNPs include ionic gelation, complex coacervation, and polyelectrolyte complexation. ChNPs have demonstrated effectiveness in bone regeneration by delivering osteogenic agents, including DNA/RNA, proteins, and therapeutics. This review provides a comprehensive analysis of recent studies on ChNPs in bone regeneration, sourced from the PubMed database. It examines their synthesis techniques, advantages as drug delivery systems, incorporation into scaffold materials, and the challenges that remain in the field.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimised solution-phase synthesis of nanoMIPs for protein detection in electrochemical diagnostics.","authors":"A N Stephen, M A Holden, M V Sullivan, N W Turner, S R Dennison, S M Reddy","doi":"10.1088/1748-605X/adb672","DOIUrl":"10.1088/1748-605X/adb672","url":null,"abstract":"<p><p>NanoMIPs are nanoscale molecularly imprinted polymers (MIPs) ranging in size between 30 to 300 nm offering a high affinity binding reagent as an alternative to antibodies. They are being extensively researched for applications in biological extraction, disease diagnostics and biosensors. Various methodologies for nanoMIP production have been reported demonstrating variable timescales required, sustainability, ease of synthesis and final yields. We report herein a fast (<2 h) method for one pot aqueous phase synthesis of nanoMIPs using an acrylamide-based monomer and N,N'-methylenebisacrylamide crosslinker. NanoMIPs were produced for a model protein template namely haemoglobin from bovine species. We demonstrate that nanoMIPs can be produced within 15 min. We investigated reaction quenching times between 5 and 20 min. Dynamic light scattering results demonstrate a distribution of particle sizes (30-900 nm) depending on reaction termination time, with hydrodynamic particle diameter increasing with increasing reaction time. We attribute this to not only particle growth due to polymer chain growth but based on AFM analysis, also a tendency (after reaction termination) for particles to agglomerate at longer reaction times. Batches of nanoMIPs ranging 400-800 nm, 200-400 nm and 100-200 nm were isolated using membrane filtration. The batches were captured serially on decreasing pore size microporous polycarbonate membranes (800-100 nm) and then released with sonication to isolate nanoMIP batches in the aforementioned ranges. Rebinding affinities of each batch were determined using electrochemical impedance spectroscopy, by first trapping nanoMIP particles within an electropolymerized thin layer. Binding constants determined for NanoMIPs using the E-MIP sensor approach are in good agreement with surface plasmon resonance results. We offer a rapid (<2 h) and scalable method for the mass production (40-80 mg per batch) of high affinity nanoMIPs.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143426667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural, microstructural, dielectric, mechanical properties of PVDF/HAP nanocomposite films for bone regeneration applications.","authors":"A P Kajal Parida, Balaram Mishra, Mukesh Kumar Gupta, Pawan Kumar","doi":"10.1088/1748-605X/adbaa4","DOIUrl":"10.1088/1748-605X/adbaa4","url":null,"abstract":"<p><p>Poly(vinylidene fluoride) (PVDF)/hydroxyapatite (HAP) nanocomposite films, incorporating HAP nanoparticles as filler within a PVDF matrix, were successfully synthesized by solution casting method. Increasing the HAP concentration in the nanocomposite significantly enhances its electroactive properties, with synergistic effects on surface, electrical and biological characteristics are investigated comprehensively. Improvements in topographical and mechanical parameters reveal the nanocomposite films for biomimetic suitability. Notably, the impact of dielectric and ferroelectric properties on biological studies is well established. With increasing the HAP concentration, we observed significant improvements in remnant polarization from 0.28 to 1.87 µC cm^-2, saturation polarization from 1.1 to 2.10 µC cm^-2, and coercive field from 88.55 to 243.65 kV cm^-1. In<i>in-vitro</i>experiments with osteosarcoma cells, the nanocomposite films with 40% HAP showed higher cell proliferation and viability. Present finding indicated 60PVDF/40HAP nanocomposite films as a biomimicry candidate for bone regeneration applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold nanoparticles/Cu decorated metal-organic frameworks for synergistic photodynamic/ferroptosis cancer therapy.","authors":"Hongchao Zhao, Hengcheng Zhu, Yang Du, Mu He, Mao Ding, Fan Cheng","doi":"10.1088/1748-605X/adba2e","DOIUrl":"10.1088/1748-605X/adba2e","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) holds promise for cancer treatment by generating reactive oxygen species via photosensitizers (PSs) activated by specific wavelengths of light. However, the poor water solubility of PSs and the tumor microenvironment, characterized by high glutathione (GSH) levels and hypoxia, limit its efficacy against hypoxic tumors. To overcome these challenges, we developed a novel nano-reactor, Zr(Cu)-MOF@Au@DHA, to augment PDT-ferroptosis therapy. By incorporating Cu²⁺into the porphyrin ring of PCN-224 and decorating it with gold nanoparticles, we enhanced the photocatalytic efficiency of the metal-organic framework (MOF). Additionally, dihydroartemisinin (DHA) was loaded onto the nano-reactor to boost the ferroptosis sensitivity of bladder cancer cells. Both<i>in vitro</i>and<i>in vivo</i>studies confirm that under laser irradiation, Zr(Cu)-MOF@Au@DHA significantly elevates oxidative stress, depletes GSH, and triggers DHA release, sensitizing tumor cells to ferroptosis and enhancing PDT-ferroptosis therapy for bladder cancer. This innovative nano-platform integrates near-infrared light-triggered PDT with chemotherapy to induce ferroptosis, addressing critical limitations in bladder cancer treatment.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of sandwich structures in biomedical applications: an innovative design for external ring fixators.","authors":"Ahmet Çetin","doi":"10.1088/1748-605X/adbb43","DOIUrl":"10.1088/1748-605X/adbb43","url":null,"abstract":"<p><p>Sandwich structures are known for their excellent strength-to-weight ratio and are being increasingly used in the automotive, aerospace, marine, and construction sectors. These structures may also offer promising designs for other fields such as biomedical fixation devices, in which the combination of lightness and stiffness is of paramount importance. This study investigated the potential of fabricating orthopedic external ring fixators from sandwich panels to develop innovative designs and broaden the scope of sandwich-structure applications. Semicircular and circular rings were fabricated from sandwich panels composed of carbon fiber composite face sheets and a polyvinyl chloride (PVC) foam core. It is evident from the available literature that epoxy/carbon fiber-reinforced composite and PVC materials with appropriate biocompatibility are used in a large number of medical devices. Layered carbon fiber composites and aluminum rings were also tested to provide a point of comparison. The mechanical results demonstrated that the sandwich rings exhibited a load-carrying capacity comparable to that of aluminum rings typically utilized in surgical procedures while exhibiting a weight reduction of approximately 60%. They provide approximately four times the mechanical advantage for specific stiffness and strength. They weigh approximately half as much as layered composite rings and are more cost-effective owing to the reduced use of carbon fibers. Furthermore, the innovative sandwich design exhibited significantly superior radiolucency compared to other rings, thus reducing the risk of confusion between the fixator and bone during stabilization and surgical operations. In summary, the sandwich rings exhibited notable improvements in load capacity, lightness, mechanical advantage, radiolucency, and cost. The proposed design has the potential to expand the applications of sandwich structures considerably, offering a cost-effective and commercially viable solution for biomedical fixators.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143525196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of zinc silicate additive on the physicochemical properties and cellular behaviors of 3D-printed magnesium phosphate bioceramic scaffolds.","authors":"Qiuyu Fu, Jiaying Xiong, Chengcheng Zhang, Zikai Li, Junxian Gan, Wenhao Huang, Wenhao Fu, Fupo He, Kunyan He, Haishan Shi","doi":"10.1088/1748-605X/adbaa2","DOIUrl":"10.1088/1748-605X/adbaa2","url":null,"abstract":"<p><p>Magnesium phosphate (MP) is a promising bone regeneration material, but it lacks the capacity to efficiently stimulate osteogenesis. In this work, zinc silicate (ZS) was used as an additive to modify MP bioceramics, and the MP-ZS composite bioceramic scaffolds with three-dimensional macroporous structure were fabricated by fiber deposition-based 3D printing and subsequent high-temperature sintering. New silicate phase was produced during sintering the MP-ZS bioceramic scaffolds. Incorporation of ZS allowed the MP-ZS scaffolds to have lower porosity and consequently higher compression strength. The MP-ZS scaffolds modified with 5 wt% ZS exhibited the highest compression strength. The MP-ZS scaffolds gradually degraded in the aqueous environment, accompanied by strength loss and pH variation. The MP-ZS scaffolds sustainably released Zn and Si ions, which could enhance cell proliferation and osteogenic differentiation. Overall, the MP-ZS bioceramic composite scaffolds could be considered as a new biomaterial for application in bone regeneration.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing osteogenic properties with gelatin/chitosan hydrogel encapsulating lithium-coated titanium oxide hollow sphere particles loaded with quercetin.","authors":"Qingjie Wang, Liang Zhang","doi":"10.1088/1748-605X/adae6e","DOIUrl":"10.1088/1748-605X/adae6e","url":null,"abstract":"<p><p>Metallic oxides especially lithium and titanium oxides are well known for their osteogenic properties. When combined in the right proportions, metallic oxides can have an even greater impact. However, releasing ions from oxides can lead to oxidative stress, which is harmful to cell growth. By reducing oxidative stress, we can enhance these ions' therapeutic and bone-forming properties. In our study, we have developed a novel combination of titanium oxide coated with lithium oxide to release ions simultaneously. We engineered hollow sphere titanium oxide particles to carry quercetin (QC), a natural antioxidant. These particles were then incorporated into a gelatin/chitosan-based hydrogel, which was further functionalized with carbon nanotubes which induced conductivity and improved mechanical properties. In drug release experiments, we found that QC was released steadily from the hydrogel, in contrast to a control group where the drug was mixed in with hydrogel indicating the significance of a secondary carrier. Additionally, our cytotoxicity tests demonstrated the importance of delivering QC alongside lithium (Li) and titanium ions, as this combination reduced toxicity and enhanced bone-forming activity. Finally, our study showed that the hydrogel containing drug-loaded hollow sphere particles could promote bone formation, as evidenced by osteogenic differentiation studies. This innovative approach holds promise for improving non-load-bearing bone regeneration therapies in the future.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking the healing effect of human Wharton's jelly stem cells labeled with superparamagnetic iron oxide nanoparticles seeded onto polyvinyl alcohol/chitosan/carbon nanotubes in burn wounds by MRI and Prussian blue staining.","authors":"Seyedeh-Sara Hashemi, Zeinab Niknam, Seyed Mojtaba Zebarjad, Davood Mehrabani, Reza Jalli, Mahdi Saeedi Moghadam, Rezvan Behroozi, Shahrokh Zare, Iman Jamhiri, Amin Derakhshanfar, Javad Moayedi, Mehra Nazempour, Aghdass Rasouli-Nia, Feridoun Karimi-Busheri, Hossein Ali Khonakdar","doi":"10.1088/1748-605X/ad9fc6","DOIUrl":"10.1088/1748-605X/ad9fc6","url":null,"abstract":"<p><p>Regenerative medicine through the application of tissue engineering and cell transplantation has provided a new door for wound healing. In this study, the healing effect of human Wharton's jelly stem cells (WJSCs) labeled with superparamagnetic iron oxide nanoparticles (SPIONs) seeded onto polyvinyl alcohol/chitosan/carbon nanotubes (PVA/CS/CNTs) in burn wounds was investigated by performing magnetic resonance imaging (MRI) and Prussian blue staining. Human WJSCs were prepared from umbilical cord and characterized. PVA/CS/CNTs were fabricated via electrospinning. Forty-eight rats were divided into four groups. The control group underwent a third-degree burn injury and was left untreated. The second group received silver sulfadiazine after burn induction, the third group was treated with PVA/CS/CNTs after burn wounds, and the fourth group received WJSCs labeled with SPIONs seeded onto PVA/CS/CNTs following burn injury. Tensile strength was investigated, real-time polymerase chain reaction was used to evaluate apoptosis, and Prussian blue staining and MRI were performed to trace labeled cells. The mesenchymal properties of WJSCs were characterized. Histologically, healing was observed as complete granulation occurred and epithelial tissues were formed in the absence of inflammatory cells, with increased expression of Bcl-2 and a decrease in Bax genes in the fourth group. Internalization of SPIONs within WJSCs was confirmed by Prussian blue staining and MRI on day 14. WJSCs labeled with SPIONs seeded onto PVA/CS/CNTs could successfully participate in the healing of burn wounds and could be easily tracked by MRI as a noninvasive method, providing a new door in regenerative medicine for burn wounds.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}