Biomedical materials (Bristol, England)最新文献

{"title":"Bio-design material as scaffold module proposed for surgical application in osteoarthritis: fabrication, characterization, biological evaluation of<i>in vitro</i>testing.","authors":"Atsadaporn Thangprasert, Anne Bernhardt, Elke Gossla, David Kilian, Saranyoo Klaiklay, Jirut Meesane, Papitchaya Srithep, Michael Gelinsky, Pakorn Pasitsuparoad","doi":"10.1088/1748-605X/ade7e4","DOIUrl":"10.1088/1748-605X/ade7e4","url":null,"abstract":"<p><p>The treatment of osteochondral defects is a major topic of current research and has become more important in our aging society. The challenges in bone and cartilage repair arise from the structure and function of these different tissues. This study proposes a biphasic model combining cartilage and bone scaffolds based on silk fibroin (SF) biopolymers. For the cartilage phase, SF scaffolds were coated with gelatin and/or agarose layers. For bone scaffolds, mineralized collagen solutions were coated on or mixed into the SF matrix. The physical and biological properties of these samples were evaluated to find the optimum conditions for a biphasic scaffold. Modification of both cartilage and bone scaffolds resulted in a smaller pore size, lower swelling rate, and higher rigidity. Gelatin significantly promoted cartilage biomarker production and agarose facilitated cell proliferation, inducing a homogeneous cell distribution and stimulating chondrogenesis. Furthermore, modification with mineralized collagen decreased cell proliferation in osteoblast progenitors but enhanced differentiation into osteoblasts. The optimum conditions were found to be a mixture of gelatin and agarose for the coating in the cartilage phase and low mineralized collagen content for the bone phase.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome as a stable carrier for anti-inflammatory phenylpropanoid metabolites: a proof-of-concept study.","authors":"Shirali Patel, Neeraja Revi, Suridh Chakravarty, Aleksandra Gurgul, Yahya Najjar, Chun-Tao Che, Katherine Mary Warpeha, Divya Bijukumar","doi":"10.1088/1748-605X/ade7e3","DOIUrl":"10.1088/1748-605X/ade7e3","url":null,"abstract":"<p><p>Phenylpropanoids (PA), which are plentiful in cruciferous vegetables, have not received adequate attention for their anti-inflammatory properties. Despite their potential benefits, the bioavailability and stability of these and other natural compounds under physiological conditions remain limited. This study aims to develop a natural nanovesicular delivery system that efficiently incorporates a phenylpropanoid extract-specifically, a multi-component anti-inflammatory extract derived from broccoli-with the goal of enhancing its bioavailability. This initiative serves as proof of concept for further research and application. The findings suggest that phenylpropanoids (PAs) achieve a 75% encapsulation efficiency within exosomes. Furthermore, it has been observed that PAs encapsulated in exosomes demonstrate a stability that is twice that of unencapsulated PAs under physiological conditions. The encapsulation process also improved the cytocompatibility of the PAs. Moreover, the functionality of the encapsulated PAs is significantly improved, as evidenced by a fivefold reduction in nitric oxide production from the EXO/PA nanocarriers. There is a significant decrease in the expression of pro-inflammatory genes, such as NFkB, MMP2, COX-2, and IL-1<i>β</i>, in comparison to cells treated with LPS. Moreover, levels of TNF-<i>α</i>, IL-6, and MCP-1 in activated macrophages treated with EXO/PAs were observed to be significantly reduced compared to those activated by LPS. It appears that the immune-suppressive effect of the extract may be mediated through both the ERK/MAPK and IkB/NFkB signaling pathways, highlighting the potential benefits of this approach. In conclusion, the results demonstrate that exosomes can effectively deliver polyphenylpropanoids while improving their stability and functionality, underscoring their potential role in future medical treatments.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aloe vera-enriched collagen-polyurethane hydrogel: supporting tissue regeneration, antibacterial action and drug release for effective wound healing.","authors":"Maria I León-Campos, Jesús A Claudio-Rizo, Juan J Becerra-Rodriguez, Laura Espindola-Serna, Lucía F Cano-Salazar, Nayeli Rodríguez-Fuentes, Rebeca Betancourt-Galindo","doi":"10.1088/1748-605X/ade7e2","DOIUrl":"10.1088/1748-605X/ade7e2","url":null,"abstract":"<p><p>Aloe vera is widely recognized for its healing, anti-inflammatory, moisturizing, and antibacterial properties, which are attributed to its rich biochemical composition, including polysaccharides, proteins, vitamins, and anthraquinones such as aloin and emodin. This study investigates the encapsulation of Aloe vera in collagen-polyurethane hydrogels to create a novel bioactive formulation for wound healing. Hydrogels with Aloe vera content ranging from 20-60 wt.% were prepared, forming scaffolds with a fibrillar-granular morphology and semi-crystalline surface. Encapsulation of Aloe vera resulted in semi-interpenetrating polymer networks (semi-IPNs), with the semi-interpenetration rate increasing as Aloe vera content rose. The formulation containing 60 wt.% Aloe vera significantly enhanced the superabsorbent capacity (2850 ± 210%) and crosslinking degree (38 ± 3%) of the matrix while reducing the loss factor (2.5 ± 0.4), resulting in a highly viscous gel ideal for moisture retention and uniform wound coverage. These hydrogels exhibited resistance to biodegradation in the presence of pepsin at skin pH (5.5) and demonstrated notable antibacterial activity, inhibiting<i>E. coli</i>(78%) and<i>S. aureus</i>(57%) growth compared to 40 ppm gentamicin. Additionally, they facilitated a gradual release of ketorolac (up to 65% at pH 5.5 with 20 wt.% Aloe vera). The hydrogels were non-hemolytic and promoted the metabolic activity of monocytes and fibroblasts, enhancing cellular growth. Furthermore, the composition induced no inflammatory response but significantly boosted the secretion of the anti-inflammatory cytokine TGF-<i>β</i>1, potentially enhancing wound healing.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loading curcumin on TiO₂nanotubes to improve surface biological activity.","authors":"Hanyu Peng, Jun Tan, Xiao Li","doi":"10.1088/1748-605X/ade488","DOIUrl":"10.1088/1748-605X/ade488","url":null,"abstract":"<p><p>Curcumin is a natural polyphenolic compound derived from turmeric, which exhibits a wide range of pharmacological activities, including anti-inflammatory and promoting bone healing effects. To enhance the bioactivity of the surface of titanium implants and promote early bone integration, the pure titanium surface was modified by composite modification through electrochemical anodic oxidation and drug coating. The surface of the prepared materials was characterized by scanning electron microscopy, atomic force microscopy, x-ray photoelectron spectroscopy, and surface contact angle analyzer. The drug release performance of the modified titanium surfaces was evaluated by ultraviolet spectrophotometry. Rat bone marrow mesenchymal stem cells (BMSCs) were extracted and identified. The effects of surface modification on cell viability were investigated through CCK-8, cell adhesion, and live/dead cell staining experiments. The effects of different surface-treated titanium sheets on osteogenic differentiation of BMSCs were evaluated by transwell assay, alkaline phosphatase activity assay, reverse transcription quantitative polymerase chain reaction, and mineralization nodule staining experiments. The results showed that successful loading of TiO₂nanotubes with curcumin was prepared, and the surface-modified titanium sheets had effective physical properties (excellent corrosion resistance, mechanical properties and hydrophilicity) and drug release capabilities. The results of<i>in vitro</i>cell culture experiments indicated that superior cell adhesion morphology was observed on the surface of each group of titanium sheets. TiO₂nanotubes and curcumin could significantly promote BMSCs proliferation and showed pleasant biocompatibility. The<i>in vitro</i>osteogenic induction differentiation experiments confirmed that the TiO₂nanotube structure and curcumin coating could promote osteogenic differentiation of BMSCs. This study provides a significant theoretical foundation and experimental support for the development of bioactive implants for dental applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spinal fusion properties of mechanically-reinforced, osteomodulatory chitosan hydrogels.","authors":"Blake T Darkow, August J Hemmerla, Joseph P Herbert, Abigail R Grisolano, Austin D Kimes, John T Wray, Mark J Messler, Julien A Lanza, Yisheng Sun, Julia R Crim, J Derek Stensby, Ryan E Schultz, Lester J Layfield, Caixia Wan, Don K Moore, Bret D Ulery","doi":"10.1088/1748-605X/ade6b8","DOIUrl":"10.1088/1748-605X/ade6b8","url":null,"abstract":"<p><p>Lower back pain is a considerable medical problem that will impact 80% of the U.S. population at some point in their lifetime. For the most severe cases, surgical repair is necessary and is associated with annual costs upwards of $10 billion in the United States alone. To alleviate back pain, spinal fusions are a common treatment in which two or more vertebrae are biologically fused together often facilitated by a graft material. Unfortunately, iliac crest bone autograft, the current gold standard graft material, can yield insufficient fusion and is associated with considerable donor site morbidity and pain as well as is in limited supply. Therefore, new materials need to be developed in order to better coordinate healing and new bone growth in the affected area to reduce unnecessary patient burden. To address this issue, we incorporated allograft (AG) and cellulose (i.e⁰CNCs and CNFs) into a dual-crosslinked chitosan hydrogel loaded with bioactive calcium phosphate was investigated. Hydrogels were then tested for both their material and biological properties. Specifically, hydrogel swelling ratio, mass loss, ion release profile, compressive strength,<i>in vitro</i>biocompatibility and osteoinduction, and<i>in vivo</i>biocompatibility and effectiveness in a spine fusion model were assessed. Cellulose and AG incorporation significantly improved hydrogel compressive strength and biocompatibility and CNFs were found to be a significantly more biocompatible form of cellulose than⁰CNCs. Additionally, through the controlled delivery of osteoinductive simple signaling molecules (i.e. calcium and phosphate ions), dibasic calcium phosphate (DCF)-loaded CNF/chitosan hydrogels were able to induce osteoblast-like activity in murine mesenchymal stem cells. When evaluated<i>in vivo</i>, these hydrogels were found to be non-toxic through the subacute phase (i.e. up to 14 d). A 6 week rabbit spine fusion study found these materials excitingly achieved near complete fusion when assessed radiographically. This research provides considerable support for the utility of our novel complex biomaterial for spine fusion procedures as well as potentially for other future bone applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144337296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxiliary Electrode Tunes Wet-Electrospun Bundle Stiffness to Modulate Cell Phenotype.","authors":"Haoyu Wang, Chelsea Violita Stanley, Xiangshen Gao, Ziyu Liu, Mo Zhou, Mingjing Zhang, Fenglei Zhou, Maryam Tamaddom, Chaozong Liu","doi":"10.1088/1748-605X/ade8c6","DOIUrl":"https://doi.org/10.1088/1748-605X/ade8c6","url":null,"abstract":"<p><p>The stiffness of tissue-engineered scaffolds significantly influences cell behaviour and phenotype. However, current approaches to tuning stiffness often introduce unintended variations and compromise topographical consistency. In this study, an innovative wet-electrospinning set-up, incorporating a positively charged auxiliary electrode was developed to fabricate bundles with adjustable stiffness. COMSOL-based electromechanical computing revealed that the auxiliary electrode provided electrostatic force, which reduced stress concentration during continuous polycaprolactone (PCL) bundle collection at speeds up to 120 m/min. Tensile testing showed that increasing collection speed significantly enhanced bundle stiffness, with Young's modulus rising from 40 to 107 MPa. X-ray diffraction analysis indicated that this strengthen effect was associated with crystal disintegration and grain refinement within PCL fibre. These changes were reflected in scaffold stiffness, thereby, further influenced cell behaviour, as bundles with higher stiffness promoted a transition from non-polarized to spindle-like cell morphology. This electrostatic-assisted collection wet-electrospun setup enables the fabrication of scaffolds with tuneable mechanical properties while preserving topographical consistency, offering a robust strategy for mechanobiology research and tissue engineering.&#xD.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144509793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxyapatite-induced bioactive and cell-imprinted polydimethylsiloxane surface to accelerate osteoblast proliferation and differentiation: an in vitro study on preparation and differentiating capacity.","authors":"Morteza Mehrjoo, Akbar Karkhaneh, Masoumeh Haghbin Nazarpak, Mostafa Alishahi, Shahin Bonakdar","doi":"10.1088/1748-605X/ade5e0","DOIUrl":"https://doi.org/10.1088/1748-605X/ade5e0","url":null,"abstract":"<p><p>Healing bone defects remains a significant orthopedic challenge. Cell therapy and tissue engineering offer promising solutions; however, obtaining high-quality, partially or fully differentiated cells remains difficult. Therefore, developing suitable substrates to guide stem cell differentiation helps in achieving this goal. Here, an optimized polydimethylsiloxane (PDMS) substrate was created by casting the PDMS composition on isolated and fixed human osteoblasts and characterizing the biological and surface features of cell patterns. A nanolayer of hydroxyapatite (nHA) was sputtered on the cell patterns to mimic the bone extracellular matrix and enhance osteo- differentiation, providing both physical and chemical stimulations. Various physical and biological properties of patterned and non-patterned PDMS substrates with and without nHA coating were evaluated to confirm the osteo-differentiation of adipose derived mesenchymal stem cells capacity. According to the results, precise cell imprinting was successfully achieved, and nHA deposition did not adversely affect the surface topography. All substrates were biocompatible, and the combination of physical (cell imprinting)-chemical (nHA coating) stimuli significantly enhanced stem cell differentiation, as evidenced by increased alkaline phosphatase activity, upregulation of bone-specific genes, and calcium deposition. A well-designed PDMS substrate can be promising for providing osteo-differentiated stem cells in large quantities for various cell therapy and tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in Nanomedicine for Cancer Theranostics.","authors":"Sudip Mukherjee, Aravind Kumar Rengan, Chitta Ranjan Patra","doi":"10.1088/1748-605X/addf26","DOIUrl":"https://doi.org/10.1088/1748-605X/addf26","url":null,"abstract":"<p><p>Cancer is one of the foremost reasons for global death. According to estimates, around 19.3 million instances of cancer and over 10 million fatalities were documented in the year 2020, making it one of the leading causes of death across the globe. There are still restrictions due to the absence of effective early detection and inadequate conventional therapy, which has led to poor prognosis and survival rates. This is the case despite the fact that there have been breakthroughs in diagnosis and treatment. The science of nanomedicine has achieved considerable advancements in the realm of cancer theranostics. These advancements offer a number of distinct advantages, including tumor-targeting through the increased permeability and retention effect, biocompatibility, and small size. In light of the above, the purpose of the Focus Issue on 'Advances in Nanomedicine for Cancer Theranostics', was introduced to highlight new research on nanomedicine-based approaches to cancer treatment. These techniques include drug and gene delivery, bioimaging, biomarker identification, diagnosis, immunotherapy, biosensors, and other precision oncology strategies. For this Focus Issue, we invited front-line researchers and authors who contributed the original research papers and topical review articles. This editorial summarizes the published articles of this collection which includes eighteen research articles and eleven review articles.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed polymeric biomaterials in bone tissue engineering.","authors":"Tianyi Xia, Xianglong Zhou, Haoran Zhou, Jiheng Xiao, Jianhui Xiang, Hanhong Fang, Liming Xiong, Fan Ding","doi":"10.1088/1748-605X/ade18d","DOIUrl":"10.1088/1748-605X/ade18d","url":null,"abstract":"<p><p>Polymers are large molecules composed of repeating subunits called monomers, which can be de-rived from both natural sources and synthetic processes. Due to their exceptional physicochemical properties and functional characteristics, polymers have garnered significant attention in the bio-medical field, particularly in tissue engineering. 3D printing technology, a process that manufactures three-dimensional objects by sequentially adding material based on digital models, has been widely recognized for its integration with polymers in bone tissue engineering (BTE). This review provides an overview of 3D-printed polymeric biomaterials in BTE. It begins with a discussion of the fundamental process of bone regeneration, followed by a component's selection for polymers and 3D printing technologies. Additionally, this review comprehensively addresses the functional properties design of 3D-printed polymeric biomaterials. Finally, the current status, challenges, and future directions for the application of 3D-printed polymeric biomaterials in BTE are discussed.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed β-TCP/curcumin scaffolds as a local drug delivery system for bone tissue regeneration.","authors":"Lilian de Siqueira, Marcela Arango Ospina, Dayane Batista Tada, Dachamir Hotza, Eliandra de Sousa Trichês, Aldo R Boccaccini","doi":"10.1088/1748-605X/ade109","DOIUrl":"10.1088/1748-605X/ade109","url":null,"abstract":"<p><p>The growing clinical need for filling defects and bone voids has led to the development of scaffolds that stimulate bone regeneration and serve as temporary models for vascularised bone growth. Additionally, these scaffolds can function as drug delivery systems to reduce inflammatory processes associated with diseases such as osteoarthritis, rheumatoid arthritis, osteoporosis, and bone cancer. Among the materials used to manufacture scaffolds,<i>β</i>-tricalcium phosphate (<i>β</i>-TCP, Ca₃(PO₄)₂) stands out due to its excellent biocompatibility and chemical composition, closely resembling minerals from bone tissue. When combined with curcumin, calcium phosphate scaffolds offer a promising platform for drug delivery, as their tailored porous structure can provide controlled release. Curcumin enhances anti-inflammatory and antioxidant properties, thereby promoting tissue regeneration. In this study,<i>β</i>-TCP powders loaded with 5 and 10 mg ml^-1of curcumin (designated as<i>β</i>-TCP/Curc 5 and<i>β</i>-TCP/Curc 10) were successfully obtained via freeze-drying and characterised using x-ray diffraction and Fourier-transform infrared spectroscopy to assess their crystallinity and chemical composition. The<i>β</i>-TCP/Curc powders were evaluated for their ability to load and release curcumin. Subsequently,<i>β</i>-TCP and<i>β</i>-TCP/Curc 5 scaffolds were prepared using 3D printing. The<i>β</i>-TCP/Curc 5 scaffolds were assessed for curcumin release, cytotoxicity profile, and antimicrobial activity. The<i>β</i>-TCP/Curc 5 powders exhibited significantly higher curcumin adsorption and good release capacity, whereas the<i>β</i>-TCP/Curc 10 powders displayed reduced curcumin loading and limited release efficiency. The combination of<i>β</i>-TCP/Curc 5 with sodium alginate produced a suitable paste for 3D printing scaffold fabrication, and the<i>β</i>-TCP/Curc 5 scaffolds demonstrated high similarity to the computational model. Importantly, the<i>β</i>-TCP scaffolds did not exhibit cytotoxicity in the MC3T3-E1 cell line, and after curcumin loading, there was no increase in cellular cytotoxicity observed. In fact, an increase in cell viability was noted compared to the control after three days of indirect assays, suggesting that this combination may be beneficial for promoting cell growth. However, the scaffolds did not show any antibacterial effects against<i>S. aureus</i>and<i>E. coli</i>under the tested conditions. This study demonstrates that adequate curcumin loading in 3D-printed<i>β</i>-TCP scaffolds can facilitate curcumin release at the bone healing site, potentially influencing the cellular processes involved in bone regeneration and remodelling.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}