Biomedical materials (Bristol, England)最新文献

{"title":"An LCD 3D-printed microphysiological system with deflectable extracellular matrix thin films.","authors":"Matt D Nelson, Patrick A Tresco, Amirhossein Razaghian, Cameron Mercer, Christian C Yost, Bruce K Gale","doi":"10.1088/1748-605X/ae8e15","DOIUrl":"10.1088/1748-605X/ae8e15","url":null,"abstract":"<p><p>Microphysiological systems (MPS) have emerged as promising models to better recapitulate<i>in vivo</i>microenvironments, but they often rely on manufacturing techniques that limit their scalability and constrain non-trivial three-dimensional architectures. Here, LCD 3D printing was used to fabricate an MPS capable of microfluidic flow, physiologically relevant cyclic strain, and resistance to rhodamine absorption. The device contains a thin, permeable (readily up to ∼70 kDa) extracellular matrix substrate (∼5 µm dehydrated) that can sustain NCI-H441 lung epithelial cells for a period of at least one week. This microfabrication strategy may be extended to different organ systems, enabling researchers to fabricate MPS with more complex designs.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional injectable BLGFS/ODEX/col hydrogel with sustained localized phage release for concurrent antimicrobial activity and bone tissue regeneration.","authors":"Bhahat L Zimba, Xiaohan Yang, Elingarami Sauli, Mwemezi Rwiza, Shenqi Wang","doi":"10.1088/1748-605X/ae8e14","DOIUrl":"10.1088/1748-605X/ae8e14","url":null,"abstract":"<p><p>Bone tissue has an inherent capacity for regeneration; however, this process is significantly impaired when the bone defects is large and infected by bacteria, particularly with multidrug-resistant strains. In this study, an injectable<i>β</i>-lactoglobulin fibrils/oxidized dextran/collagen (BLGFs/ODEX/COL) hydrogel biomaterial embedded with<i>Acinetobacter baumannii</i>bacteriophages was developed and evaluated for treating infected partial-thickness cortical tibia bone defects in rat models. The bacteriophage was successfully isolated from hospital sewage, amplified, purified, and morphologically confirmed using TEM. The SEM results showed that the hydrogel exhibited a highly porous and interconnected structure with pore sizes exceeding 50 µm, conducive for cell infiltration and tissue regeneration. ATR-FTIR analysis verified the chemical interactions amongst BLGFs, ODEX, and COL, confirming successful hydrogel formation.<i>In vivo</i>results demonstrated that bone defects treated with the phage-loaded hydrogel showed significantly accelerated bone regeneration by day 21 compared to antibiotic-treated and phage-only groups. Micro-CT analysis revealed superior outcomes in the hydrogel group, including higher tissue volume (above 4000 mm³), increased percent bone volume (above 2.4%), and greater bone surface density (above 0.1 mm^-1), indicating enhanced osteogenesis and bone remodeling despite infection. The incorporation of bacteriophages into the BLGFs/ODEX/COL hydrogel provides a synergistic therapeutic strategy by combining localized antimicrobial activity with structural and biological support, thereby promoting improved healing of infected bone defects.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148551056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of the bioactive PLGA/CaP composites: the influence of 3D structure on inflammatory and osteoinductive properties.","authors":"Andrei V Yushkov, Ekaterina A Kuvshinova, Inna N Bulygina, Vasilina A Zakharova, Roza Yu Saryglar, Igor V Vakhrushev, Elizaveta V Koudan","doi":"10.1088/1748-605X/ae9233","DOIUrl":"10.1088/1748-605X/ae9233","url":null,"abstract":"<p><p>We aimed to develop a composite poly (lactic-co-glycolic acid) (PLGA)/calcium phosphate nanoparticles scaffold with the optimal three-dimensional structure to provide an environment for bone tissue regeneration. Composite PLGA-based scaffolds with the inclusion of 15% hydroxyapatite (HA) and<i>β</i>-tricalcium phosphate (<i>β</i>-TCP) nanoparticles, as well as scaffolds with the addition of 15% xenogeneic bone chips, and with different pore diameters were prepared by a solvent casting with particle leaching method. Synthesized HA and<i>β</i>-TCP nanoparticles were characterized using x-ray phase analysis and atomic force microscopy. The scaffold morphology was studied with electron microscopy and energy dispersive x-ray spectroscopy. The scaffold biocompatibility, immunogenicity, inflammatory and osteoinductive properties were investigated<i>in vitro</i>using dental pulp stem cells (DPSCs), human lymphocyte culture, and RAW 264.7 mouse macrophage cells. Among the investigated samples, the PLGA/<i>β</i>-TCP scaffold showed the highest osteoinduction, and the scaffold with 530 ± 56 μm average pore diameter demonstrated the highest expression of osteodifferentiation marker genes in DPSCs. Furthermore, the addition of nanoparticles into the polymer matrix led to the decrease in the expression of pro-inflammatory genes in macrophages. PLGA with 15%<i>β</i>-TCP and 530 ± 56 μm pore size had the best bioactivity among the tested scaffolds<i>in vitro</i>, and it could be considered as a potential candidate for bone tissue engineering applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148622854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology for targeted modulation of mitophagy: molecular mechanisms, therapeutic applications, and translational perspectives.","authors":"Jiaenli Bolati, Junjie Yang, Yueqi Yan, Donghua Yu, Chunmiao Yu","doi":"10.1088/1748-605X/ae95c8","DOIUrl":"10.1088/1748-605X/ae95c8","url":null,"abstract":"<p><p>Mitophagy is a selective autophagic process responsible for the elimination of damaged or dysfunctional mitochondria, playing a critical role in maintaining mitochondrial quality control and cellular homeostasis. Dysregulated mitophagy has been implicated in the pathogenesis of numerous diseases, including neurodegenerative diseases, cancer, cardiovascular diseases, metabolic disorders, inflammatory and immune diseases, and musculoskeletal diseases. In recent years, nanotechnology-based approaches for the targeted modulation of mitophagy have emerged as promising therapeutic strategies due to their ability to achieve precise regulation, enhanced subcellular targeting, and reduced off-target effects. Building upon advances in the understanding of mitophagy mechanisms, a variety of nano-delivery systems have been developed, incorporating strategies such as mitochondria-targeting, stimuli-responsive activation, ligand-mediated targeting, and combination therapies. However, a comprehensive review integrating the molecular mechanisms of mitophagy, disease-specific therapeutic applications, nanoplatform design strategies, and translational challenges remains lacking. The present review provides an integrated overview of the molecular regulatory mechanisms of mitophagy, summarizes recent advances in nanotechnology-based therapeutic interventions across multiple disease types, and critically discusses current nanoplatform design strategies. Furthermore, key challenges associated with mitophagy evaluation, biosafety, pharmacokinetics, clinical translation, and regulatory considerations are highlighted, together with emerging technologies that may accelerate the development of next-generation mitophagy-targeting nanomedicines.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"21 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148802038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed titanium scaffolds negatively affect the interplay between macrophages and skeletal progenitors<i>in vitro</i>.","authors":"Gabriele Nasello, Edoardo Borgiani, Tim Herpelinck, Giulia Rossana Gulino, Liesbet Geris","doi":"10.1088/1748-605X/ae95a9","DOIUrl":"10.1088/1748-605X/ae95a9","url":null,"abstract":"<p><p>Orthopedic implants can guide endogenous tissue regeneration, with outcomes shaped by early interactions between immune cells and tissue specific progenitors. While interest in the osteoimmunomodulatory function of 3D printed bone scaffolds is increasing, most studies focus on the effects of immune cells on bone forming cells, often overlooking the immunomodulatory role of skeletal progenitors. This study examines how 3D printed titanium scaffolds influence macrophage polarization mediated by skeletal progenitors and how the resulting macrophage response affects osteogenic differentiation of these progenitors. An indirect co culture system was used, with primary human macrophages seeded on titanium scaffolds and human periosteum derived cells cultured separately. After five days, conditioned media were exchanged. Titanium scaffolds promoted an inflammatory macrophage profile and limited the modulatory effect of skeletal progenitors under specific readouts. In turn, macrophage conditioned media from scaffold cultures selectively altered osteogenic gene expression in skeletal progenitors, with effects on SPP1 and COL1A1. These findings indicate that 3D printed titanium scaffolds influence macrophage-progenitor crosstalk that may affect regenerative signaling.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148681381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human gastric extracellular matrix hydrogels maintain organoid growth and reduce gene expression associated with stemness and inflammation.","authors":"Michelle D Cherne, Jasper L Gattiker, Katrina N Lyon, Daniella Russell, Thomas A Sebrell, Alexis Burcham-Carter, Ava P Richard, Eli N Mathew, Samuel G Mackintosh, Andrew J Gorzalski, Diane Bimczok","doi":"10.1088/1748-605X/ae943e","DOIUrl":"10.1088/1748-605X/ae943e","url":null,"abstract":"<p><p>Human gastric organoids (HGOs) are 3D cultures of primary gastric epithelial cells that serve as in vitro models for gastric epithelial physiology, development, and disease. The extracellular matrix (ECM) material that organoids are maintained in provides crucial signals to the epithelial cells, regulating their function and growth. We here describe the application of human gastric ECM (hgECM) for HGO cultures, to provide a tissue- and species-specific growth matrix. We prepared hgECM from human gastric tissue by decellularization and then compared HGO growth, differentiation, and function in hgECM and in the traditionally used Matrigel. The composition, structure, and rheological properties of the hgECM were determined using tandem mass spectrometry, scanning electron microscopy, and rheometry. Transcriptional profiles of HGOs grown in hgECM and Matrigel were compared using single cell RNA sequencing (scRNAseq). Proteome analysis showed that hgECM was rich in collagens, whereas Matrigel was predominantly composed of glycoproteins such as laminin. The structure of hgECM was fibrous, befitting its collagen composition, while Matrigel was porous. The viscosity and stiffness of hgECM were found to be lower than Matrigel. HgECM supported robust growth of HGOs. ScRNAseq showed that cell type composition of HGOs in each ECM was comparable. However, HGOs in hgECM had reduced expression of genes associated with stem cells, epithelial-mesenchymal transition, stress, and inflammation compared to HGO grown in Matrigel. Conversely, HGO culture in hgECM increased expression of gastric tissue-specific genes and of genes associated with growth and metabolism. HgECM hydrogels were highly compatible with HGO culture, and improved homeostatic functions of HGOs, including growth, differentiation, and reduced inflammation. HgECM may serve as a tissue- and species-specific growth matrix for HGO culture to improve the physiological relevance of organoid models.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13478677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148671333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endotoxin-resistant dendritic cell-derived biomimetic exosomes loaded iridium nanoenzymes for alleviating LPS/D-GalN-induced acute liver failure.","authors":"Xueting Xie, Shuqi Shen, Ziyin Xie, Shunfu Wang, Yue Si, Kaixin Zhu, Huifang Shi, Chenyi Liu, Shu Li, Shiwen Wu, Jinghan Hu, Yang Dai, Wenzhang Jin, Mingqin Lu","doi":"10.1088/1748-605X/ae94cd","DOIUrl":"10.1088/1748-605X/ae94cd","url":null,"abstract":"<p><p>Acute liver failure (ALF) is characterized by a rapid deterioration of liver function in a very short period of time, with a variety of etiological factors and complex pathogenic mechanisms, for which there are no effective therapeutic options. Recent studies have demonstrated that endotoxin-tolerant dendritic cells (ETDC) possess anti-inflammatory properties, typically established in response to repeated lipopolysaccharide (LPS) stimulation. However, living cells are extremely unstable<i>in vivo</i>, so we fabricated exosome mimics (EM), which have higher yields and are simpler and more convenient to handle than conventional exosome properties. Polyvinylpyrrolidone-stabilised iridium nanoparticles (Ir NPs-PVP) have been reported as enzyme mimics with peroxidase- and catalase-like activities and have been shown to be effective in scavenging reactive oxygen species at the cellular level. The aim of this paper is to investigate the palliative effect of ETDC-derived mimetic vesicles loaded with PVP-IrNPs on ALF. The results showed that the combination of ETDC-derived mimetic vesicles and Ir NPs-PVP had anti-inflammatory and antioxidant effects, which effectively alleviated the damage induced by ALF, and is expected to be a novel therapeutic agent for the clinical treatment of ALF.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148703049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous scaffolds for vascularized bone regeneration: a review of recent advances.","authors":"Xinyue Liu, Duoling Xu, Chao Wang","doi":"10.1088/1748-605X/ae8ee7","DOIUrl":"10.1088/1748-605X/ae8ee7","url":null,"abstract":"<p><p>Bone tissue defects, which are frequently caused by tumors, infections, traumas, and developmental malformations, have been recognized as significant clinical challenges in tissue regeneration. The development of porous scaffolds that demonstrate both effective vascularized bone regeneration capability and mechanical compatibility with native bone tissue has emerged as a prominent research focus. It has been well-established that the osteogenic and vascularization potential of porous scaffolds is predominantly influenced by their material composition, fabrication techniques, and physicochemical properties. In this review, a comprehensive analysis was conducted on the multifactorial mechanisms through which porous scaffolds affect osteogenesis and vascularization processes. The current understanding of scaffold-mediated bone regeneration was systematically evaluated, with particular emphasis on the optimization of scaffold characteristics. This review aims to provide new perspectives and theoretical foundations for the application of porous scaffolds in bone tissue defect repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148563957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage proteome modulation induced by tribocorrosion of graphene oxide-hyaluronic acid-coated CoCr surfaces.","authors":"L Sánchez-López, B Chico, M C García-Alonso, R M Lozano","doi":"10.1088/1748-605X/ae92e2","DOIUrl":"10.1088/1748-605X/ae92e2","url":null,"abstract":"<p><p>Hip and knee implant components, such as femoral bearings, acetabulum liners, and stem taper junctions are subjected to mechanical stresses and undergo electrochemical processes that compromise their functionality. Cobalt-chromium (CoCr) alloys are widely used as implants owing to their excellent mechanical properties, corrosion resistance, and biocompatibility. However, they are susceptible to simultaneous surface processes of wear and corrosion, known as tribocorrosion, which reduce their longevity and impact their performance. Our group has developed covalent multifunctional nanocoatings of graphene oxide (GO) and hyaluronic acid (HA) on CoCr surfaces providing chemical protection, reducing inflammatory response and enhancing wear-corrosion resistance. However, when the GO/HA nanocoating is disrupted by tribocorrosion, as takes place under<i>in vivo</i>conditions, macrophages, crucial cells for inflammation and healing, could exhibit a significantly different proteome. For this purpose, CoCr modified GO/HA surfaces were configured in a tribometer to simulate tribocorrosion of CoCrMo/CoCrMo joint implants, and the biological effects were studied in murine macrophages and analyzed via high-throughput proteomics. Simulated tribocorrosion testing revealed that metal degradation activates phagocytosis and increases endo-lysosomal membrane proteins, indicating enriched phagosomal compartment formation in macrophages. Additionally, macrophage exposure to worn CoCr-modified surfaces activates metal sensitization mechanisms and antigen presentation, leading to macrophage activation and polarization.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148632946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Syringeable hyaluronic acid-based hydrogel co-loaded with cisplatin and MSA-2 for enhanced ovarian cancer chemoimmunotherapy.","authors":"Zhengfei Sun, Dehua Wei, Guofeng Ji","doi":"10.1088/1748-605X/ae8b4c","DOIUrl":"10.1088/1748-605X/ae8b4c","url":null,"abstract":"<p><p>Low immunogenicity and a prevailing immunosuppressive tumor microenvironment (TME) remain major bottlenecks for ovarian cancer (OC) immunotherapy. While platinum-based chemotherapy can trigger antitumor immunity via immunogenic cell death (ICD), its clinical efficacy is often hampered by the intrinsic immunosuppressive milieu and insufficient drug accumulation at the tumor site following systemic administration. To address these challenges, we fabricated a syringeable hyaluronic acid-based hydrogel co-loaded with Cisplatin (CDDP) and STING agonist MSA-2 (CDDP/MSA-2@Gel) for enhanced localized chemoimmunotherapy. The sustained local release of CDDP and MSA-2 synergistically boost stimulator of interferon genes (STING) pathway activation, thereby eliciting potent type-I-IFN-driven systemic antitumor immune responses and alleviating the immunosuppressive TME.<i>In vivo</i>studies demonstrated that CDDP/MSA-2@Gel treatment significantly inhibits tumor growth in murine OC models without systemic toxicity. Collectively, our designed CDDP/MSA-2@Gel represents a safe and potent strategy for enhanced synergistic chemoimmunotherapy, offering significant potential for clinical translation in the treatment of OC.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}