Biomedical materials (Bristol, England)最新文献

{"title":"Study on the development of nanoparticles based on levan for oral insulin delivery.","authors":"Desy Kurniawati, Neng Fisheri Kurniati, Enny Ratnaningsih, Rukman Hertadi","doi":"10.1088/1748-605X/adb22d","DOIUrl":"https://doi.org/10.1088/1748-605X/adb22d","url":null,"abstract":"<p><p>Oral insulin administration has gained attention as a promising alternative to injections. However, its effectiveness is hindered by the major challenge of degradation by gastric acid. Biopolymer-based nanocarriers have been explored as a solution to address this challenge. This study examines levan, a biopolymer derived from<i>Bacillus licheniformis</i>BK1, for its viability as a nanocarrier for insulin. Levan was modified through acetylation, and both levan (I-Lv) and its acetylated (I-ALv) form were utilized as carriers for insulin in a nanoparticles (NPs) delivery system. The resulting NPs were spherical, with diameters ranging from 250 to 500 nm and encapsulation efficiencies of 78.64% and 88.30%, respectively. The insulin release from I-Lv NPs in simulated gastric fluid exhibited a burst release pattern that was more rapid than that of I-ALv. To further evaluate, the conformational stability of insulin in NPs was analyzed by measuring the transition enthalpy of secondary and tertiary structures. The stability of the secondary structure was determined through alpha-helix content using circular dichroism, while the tertiary structure stability was evaluated via the fluorescence intensity of tryptophan residues. The result revealed that insulin in I-ALv NPs exhibited enhanced conformational stability compared to free-state (FS) insulin and I-Lv NP, with transition enthalpies of 0.91 ± 0.62 and 4.42 ± 0.46 kcal mol^-1for secondary and tertiary structures, respectively. Moreover, preliminary<i>in vivo</i>studies revealed that I-ALv had a significant impact compared to FS insulin and I-Lv, demonstrating reduction in blood glucose levels. These findings highlight the potential of I-ALv as a promising candidate for antidiabetic therapy and an efficient oral delivery system.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":"20 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143460887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Ficus carica</i>leaves extract-loaded PLGA nanoparticles: preparation, characterization, and<i>in vitro</i>anticancer activity on TFK-1 cell line.","authors":"Bushra Aziz, Esmeralda Dc Bosman, Kim Mg van der Wurff-Jacobs, Cornelus F van Nostrum, Ahmat Khurshid","doi":"10.1088/1748-605X/adaff7","DOIUrl":"10.1088/1748-605X/adaff7","url":null,"abstract":"<p><p><i>Ficus carica</i>extract (FCe) is a natural herb that has received a lot of interest in cancer treatment due to its potential anticancer activities against various malignancies. However, due to FCe's low bioavailability and low solubility, its clinical use as an anti-cancer medicine is constrained. The current study aimed to prepare FCe-loaded poly(lactic-<i>co</i>-glycolic acid) (PLGA) nanoparticles (NPs) for cancer treatment. Prepared NPs were characterized by UV-v is spectroscopy, dynamic light scattering, zeta potential, and transmission electron microscopy. The results showed that the spherical FCe-loaded PLGA NPs had a particle size of 162 ± 0.7 nm, a polydispersity index of 0.08 ± 0.005, and a zeta potential of -4.7 ± 0.6 mV. The encapsulation and loading efficiency were found to be 56 ± 2.3% and 14 ± 1.5%, respectively. A drug release study indicated a diffusion-based release profile. Cytotoxicity was evaluated on the extrahepatic bile duct carcinoma (TFK-1) cell line, which showed that both free FCe and corresponding FCe concentrations in NPs were cytotoxic. Cell cycle analysis showed that the FCe arrests the cells in G0/G1 phase, and the cell arrest rate is higher in FCe-loaded NPs compared to free form. A phototoxicity study also showed that the phototoxicity of FCe-loaded PLGA NPs was time-dependent and enhanced in comparison to free FCe. The study's results demonstrated that FCe-encapsulated PLGA NPs are promising for cancer therapy as a phyto- and phototherapeutic agent-based system.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed polycaprolactone/poly (L-lactide-co-<i>ϵ</i>-caprolactone) composite ureteral stent with biodegradable and antibacterial properties.","authors":"Yanjiao Teng, Xinyan Wang, Lin Song, Jianing Yang, Shike Hou, Qi Lv, Li Jiang, Yong Guan, Jie Shi","doi":"10.1088/1748-605X/adb2ce","DOIUrl":"10.1088/1748-605X/adb2ce","url":null,"abstract":"<p><p>The clinical application of biodegradable ureteral stents holds significant potential. There is an urgent need to develop new materials for ureteral stents to address the limitations related to performance degradation and antibacterial properties observed in current designs. Here, we developed a Polycaprolactone (PCL)/Poly (L-lactide-co-<i>ϵ</i>-caprolactone) (PLCL) composite ureteral stent by three-dimensional (3D) printing, which exhibits biodegradable and antibacterial properties. Silver nanoparticles (AgNPs) were bonded to the surface of the stent through the polymerization of dopamine (PDA) and coating with type I collagen (Col I). The ureteral stent (PP-PDA-Ag-Col) had a densely spiraled structure and higher hydrophilicity. The release behavior of silver ions from the stent was found to be slow and continuous when coated with AgNPs, which can enable long-term antibacterial effects after being implanted<i>in vivo</i>. Additionally,<i>in vitro</i>degradation experiments demonstrated that the different ratios of ureteral stents degraded slowly in artificial urine over 6 weeks without compromising functionality. The stent exhibits excellent hemocompatibility and cell compatibility. The subcutaneous implantation experiment in Sprague-Dawley rats showed that the PP-PDA-Ag-Col stent degraded slowly<i>in vivo</i>and had good biocompatibility. The stent PCL5/PLCL5 was the most promising ureteral stent regarding antibacterial, mechanical properties, and degradation. The novel 3D-printed PP-PDA-Ag-Col stent exhibits biocompatibility for safe<i>in vivo</i>transplantation and antibacterial properties that reduce reliance on antibiotics. Additionally, its biodegradability eliminates the need for secondary surgical removal, making it a promising option for the clinical application of ureteral stents.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of cellular pattern organization and clarity through centrifugal force.","authors":"Lauren E Mehanna, James D Boyd, Shelley Remus-Williams, Nicole M Racca, Dawson P Spraggins, Martha E Grady, Brad J Berron","doi":"10.1088/1748-605X/ada508","DOIUrl":"10.1088/1748-605X/ada508","url":null,"abstract":"<p><p>Rapid and strategic cell placement is necessary for high throughput tissue fabrication. Current adhesive cell patterning systems rely on fluidic shear flow to remove cells outside of the patterned regions, but limitations in washing complexity and uniformity prevent adhesive patterns from being widely applied. Centrifugation is commonly used to study the adhesive strength of cells to various substrates; however, the approach has not been applied to selective cell adhesion systems to create highly organized cell patterns. This study shows centrifugation as a promising method to wash cellular patterns after selective binding of cells to the surface has taken place. After patterning H9C2 cells using biotin-streptavidin as a model adhesive patterning system and washing with centrifugation, there is a significant number of cells removed outside of the patterned areas of the substrate compared to the initial seeding, while there is not a significant number removed from the desired patterned areas. This method is effective in patterning multiple size and linear structures from line widths of 50-200 μm without compromising immediate cell viability below 80%. We also test this procedure on a variety of tube-forming cell lines (MPCs, HUVECs) on various tissue-like surface materials (collagen 1 and Matrigel) with no significant differences in their respective tube formation metrics when the cells were seeded directly on their unconjugated surface versus patterned and washed through centrifugation. This result demonstrates that our patterning and centrifugation system can be adapted to a variety of cell types and substrates to create patterns tailored to many biological applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11823422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of mesoporous core-shell bioactive glass-based cements for direct pulp capping.","authors":"Xiaoting Xu, Yehong He, Xiaochen Chen, Zhen Fan, Deping Wang","doi":"10.1088/1748-605X/adb119","DOIUrl":"10.1088/1748-605X/adb119","url":null,"abstract":"<p><p>Direct pulp capping is crucial for maintaining dental pulp vitality. The materials employed for this purpose should possess properties such as easy-handling, excellent biocompatibility, infection resistance, and the ability to stimulate the formation of reparative dentin. Mineral trioxide aggregate (MTA) is commonly used for pulp capping. However, certain limitations, including its long setting time, insufficient anti-washout ability, high initial curing pH, and handling difficulties, restrict MTA from meeting a broader range of clinical demands. Bioactive glass (BG) is known for its osteo-inductive and bone restoration properties. This study aims to develop a novel BG-based cement (BG-<i>x</i>/SA) for pulp capping applications, using BG microspheres (BG-<i>x</i>) as the solid phase and sodium alginate solution (SA) as the liquid phase. The solid phase has core-shell microspheres made by<i>in-situ</i>transformation, with BG in the core and hydroxyapatite in the shell. The study focuses on how the<i>in-situ</i>synthesis reaction parameters affect the cement's properties. Ultimately, BG-6/SA cement was identified as the optimal formulation, and a comparison with MTA shows BG-6/SA cement has a short setting time, good anti-washout performance, can adjust pH to mildly alkaline, promotes dentin formation, and has antibacterial effects. Thus, BG-6/SA cement has significant research value and good prospects as a new pulp-capping material.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterization of TiO₂-ZnO composite thin films for biomedical applications.","authors":"Gimmi Guruprasad Engoor, Vimal Kumar Dewangan, Sachin Latiyan, T S Sampath Kumar, N Sujatha, I A Palani, Nilesh J Vasa","doi":"10.1088/1748-605X/adb118","DOIUrl":"10.1088/1748-605X/adb118","url":null,"abstract":"<p><p>Titania (TiO₂) has superior biocompatibility, while zinc oxide (ZnO) is antibacterial. This investigation aimed to study the influence of TiO₂-ZnO composite films on enhancing the biocompatibility of stainless steel (SS). Radio-frequency magnetron sputtering (RF-MS) technique is used to synthesize TiO₂-ZnO composite thin films on 304-SS substrates from three sputtering targets with typical chemical compositions of 100% TiO₂, 90%TiO₂-10%ZnO, and 75%TiO₂-25%ZnO, mixed by their respective weight percentages. The influence of surface chemistry, morphology, and wettability of TiO₂-ZnO composite film on its osseointegration and antifouling characteristics was studied. The biocompatibility was assessed by protein adsorption kit, cytotoxicity assay, and cell adhesion of MG63 osteoblast cells, followed by<i>S. aureus</i>bacterial adhesion studies. All RF-MS films displayed hydrophobicity, minimal bacterial-cell adhesion, and higher cytocompatibility than the SS. RF-MS films deposited from the 75%TiO₂-25%ZnO target exhibited the highest antifouling capability due to the least protein adsorption and the highest antibacterial ZnO concentration. However, increased ZnO concentration decreased MG63 cell viability. RF-MS films deposited from the 90%TiO₂-10%ZnO target showed the highest mammalian cell viability of ≈88% and attachment. High plasma protein adsorption caused decreased mammalian cell viability and higher bacterial adhesion on 100% TiO₂film and SS. Biocompatible and antifouling TiO₂-ZnO composite thin films on SS substrates offer an alternative to conventional antibiotic coatings to combat antimicrobial resistance (AMR) and biofilm-related infections.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional cell culture-derived extracellular vesicles loaded alginate/hyaluronic acid composite scaffold as an optimal therapy for cartilage defect regeneration.","authors":"Wanting Zhang, Shuyi Li, Yingying Peng, Zhujie Deng, Quanjiang Li, Rui Tian, Xiubin Kuang, Yuyi Kang, Ronghui Sun, Chen Huang, Zhengqiang Yuan","doi":"10.1088/1748-605X/adb22e","DOIUrl":"10.1088/1748-605X/adb22e","url":null,"abstract":"<p><p>Osteoarthritis (OA) is a chronic musculoskeletal disease characterized by joint inflammation and progressive degeneration of articular cartilage. Currently a definitive cure for OA remains to be a challenge due to the very low self-repair capacity of cartilage, thus development of more effective therapies is needed for cartilage repair. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have shown great potential as therapeutic agents for stimulating regeneration of articular cartilage. However, a standardized protocol is still lacking for manufacturing of highly active EVs for clinical applications. This study aimed to investigate the efficient production of highly active EVs by 3-dimensional (3D) MSC culture, verify the reparative efficacy of EVs on cartilage defect and elucidate the repair mechanisms. Umbilical cord MSCs were embedded in alginate to form MSC spheroids for 3D culture in human platelet lysate (hPL)-containing medium, which produced 3D culture-derived EVs (3D-EVs) with a significantly improved yield. The 3D-EVs expressed higher level of VEGF, and appeared superior to two-dimensional (2D) monolayer MSC culture-derived EVs (2D-EVs) to improve migration and proliferation in MSCs and inflammatory chondrocytes, and to suppress expression of cartilage-degrading factors. Importantly, the 3D-EVs and sodium alginate (SA)-hyaluronic acid (HA) composite hydrogel (3D-EVs/SA-HA) demonstrated significantly improved therapeutic efficacy than 2D-EVs/SA-HA hydrogel for repair of cartilage defect<i>in vivo</i>. The underlying mechanisms are associated with the concomitant upregulation of type II collagen and cartilage synthesis and downregulation of MMP13 in cartilage tissues. Collectively, these data showed that highly active MSC EVs could be efficiently manufactured by 3D cell culture with hPL-containing medium, and these EVs were superior to 2D-EVs for the repair of articular cartilage defect.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application and development prospects of extracellular vesicles in oral administration.","authors":"Weiqun Li, Ming-Jie Dong, Zhaoqing Shi, Haibing Dai, Shanming Lu, Huibin Wu, Fan Zhang, Guihong Lu, Jianbo Yu","doi":"10.1088/1748-605X/adb22c","DOIUrl":"10.1088/1748-605X/adb22c","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are nanoscale phospholipid-based particles secreted by cells and are essential mediators responsible for intercellular signal communication. The rapid development of EV nanotechnology has brought unprecedented opportunities for nanomedicine. Among various administration methods, oral administration is the most convenient and simplest. However, most drugs (peptides, small molecule drugs, nucleic acids, and therapeutic proteins) greatly reduce their oral bioavailability due to the harsh gastrointestinal environment. Notably, some EVs have been shown to cross biological barriers, including the gastrointestinal tract. The distinctive biological properties of EVs make them a promising natural carrier for oral drug delivery. This review introduces the characteristics of EVs, covering their classification, production methods, and therapeutic efficacy in oral administration. Additionally, we explore the potential roles of EVs in disease prevention and treatment, as well as their future prospects in pharmaceutical applications. This comprehensive overview aims to provide insights into the application of EVs in oral drug delivery, highlighting their advantages, current progress, and the challenges that need to be overcome for successful clinical translation.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan and ibuprofen grafted electrospun polylactic acid/gelatin membrane mitigates inflammatory response.","authors":"Qiaolin Ma, Anlin Yin, Xinjian Wan, Binbin Sun, Hongsheng Wang, Mohamed El-Newehy, Meera Moydeen Abdulhameed, Xiumei Mo, Jinglei Wu, Tian Tu","doi":"10.1088/1748-605X/adae48","DOIUrl":"10.1088/1748-605X/adae48","url":null,"abstract":"<p><p>Electrospun membranes with biomimetic fibrous structures and high specific surfaces benefit cell proliferation and tissue regeneration but are prone to cause chronic inflammation and foreign body response. To solve these problems, we herein report an approach to functionalize electrospun membranes with antibacterial and anti-inflammatory components to modulate inflammatory responses and improve implantation outcomes. Specifically, electrospun polylactic acid (PLA)/gelatin (Gel) fibers were grafted with chitosan (CS) and ibuprofen (IBU) via carbodiimide chemistry. Our results show that the surface modification strategy endows electrospun membranes with moderate antibacterial activities and sustained release of anti-inflammatory drugs. The electrospun PLA/Gel-CS-IBU membrane showed good antioxidant and anti-inflammatory activity as evidenced by suppressing M1 polarization and promoting M2 polarization of macrophages<i>in vitro</i>. Similarly, it induced significantly milder chronic inflammatory responses<i>in vivo</i>than unmodified electrospun membranes. Given the good anti-inflammatory and antibacterial effects, this strategy might improve the biological performance of electrospun membranes as implants in clinics.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermal-crosslinked acellular dermal matrix combined with adipose-derived stem cells to regenerate vascularized adipose tissue.","authors":"Xing Huang, Siyuan Chen, Lin Lu, Rui Jin, Mengling Chang, Zhaoqi Yuan, Xusong Luo, Zhu Zhu, Guangpeng Liu","doi":"10.1088/1748-605X/adaff8","DOIUrl":"10.1088/1748-605X/adaff8","url":null,"abstract":"<p><p>The reconstruction of large-sized soft tissue defects remains a substantial clinical challenge, with adipose tissue engineering emerging as a promising solution. The acellular dermal matrix (ADM), known for its intricate spatial arrangement and active cytokine involvement, is widely employed as a scaffold in soft tissue engineering. Since ADM shares high similarity with decellularized adipose matrix, it holds potential as a substitute for adipose tissue. This study explores the adipogenic ability of a spongy material derived from ADM via vacuum-thermal crosslinking (T-ADM), characterized by high porosity, adjustable thickness, and suitable mechanical strength. Adipose-derived stem cells (ADSCs) are considered ideal seed cells in adipose tissue engineering. Nevertheless, whether pre-adipogenic induction is necessary before their incorporation remains debatable. In this context, ADSCs, both with and without pre-adipogenic induction, were seeded into T-ADM to regenerate vascularized adipose tissue. A comparative analysis of the two constructs was performed to evaluate angiogenesis and adipogenesis<i>in vitro</i>, and tissue regeneration efficacy<i>in vivo</i>. Additionally, RNA-seq analysis was utilized to investigate the potential mechanisms. The results showed that T-ADM exhibited good performance in terms of volume retention and maintenance of adipocyte phenotype, confirming its suitability as a scaffold for adipose tissue engineering.<i>In-vitro</i>outcomes demonstrated that pre-adipogenic induction enhanced the adipogenic level of ADSCs, but reduced their ability to promote vascularization. Furthermore, constructs utilizing pre-induced ADSCs showed an insignificant superiority in<i>in-vivo</i>fat formation, and neovascularization compared with those with non-induced ADSCs, which may be attributed to similar macrophage regulation, and balanced modulation of the proliferator-activated receptor-<i>γ</i>and hypoxia-inducible factor 1<i>α</i>pathways. Consequently, the direct use of ADSCs is advocated to streamline the engineering process and reduce associated costs. The combined strategy of T-ADM with ADSCs proves to be feasible, convenient and effective, offering substantial potential for addressing large-sized tissue deficits and facilitating clinical applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143069995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}