海参蛋白膏对小鼠皮肤创面愈合的影响及其潜在的抗炎机制。

Xiaoyan Wang, Jinghe Sun, Ke Liu, Shuang Li, Jun Zhao, Jingfeng Yang
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引用次数: 0

摘要

具有抗炎活性的天然物质一直是治疗人体损伤的首选物质。本研究旨在通过BALB/c小鼠模型和lps诱导的RAW 264.7细胞,探讨海参蛋白(SCP)对创面愈合的有益作用及其机制。为了了解SCP膏体的工作原理,我们鉴定了小鼠的血清细胞因子和组织切片。探讨了SCP抗炎作用过程中NF-κB通路的变化。结果显示,SCP(H)组创面愈合率超过90%,而对照组和NC组创面愈合率分别为72.91%和64.10%。创面生成了新的血管和成纤维细胞。第14天,与对照组相比,SCP(L)组和SCP(H)组胶原蛋白表达量分别提高了13.89%和15.12%。此外,SCP降低小鼠血清中促炎因子(TNF-α、IL-1β、IL-6)水平,上调愈合过程中抗炎因子(IL-10)水平。此外,SCP通过降低磷酸化p65和IKKα的蛋白水平,增加ikb α的蛋白水平来抑制NF-κB通路。 。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of sea cucumber protein paste for mice's skin wound healing and its potential anti-inflammatory mechanism.

Natural substances with anti-inflammatory activity have always been the priority for human injuries. This study aims to investigate the beneficial effects and mechanism of sea cucumber protein (SCP) on wound healing, through a BALB/c mouse model and lipopolysaccharides-induced RAW 264.7 cells. We identified the mice's serum cytokines and tissue section to find out how SCP paste works. The alteration of the nuclear factor-κB (NF-κB) pathway during the anti-inflammatory effect of SCP was also explored. The results showed that the wound healing rate in the SCP(H) group exceeded 90%, whereas it was 72.91% and 64.10% in the Control and negative control groups on day 14. New blood vessels and fibroblasts were generated in the wounds. Collagen expression increased by 13.89% and 15.12% respectively in the SCP(L) and SCP(H) groups compared with the Control group on day 14. Furthermore, SCP decreased the levels of pro-inflammatory factors (tumor necrosis factor-α, interleukin (IL)-1β, IL-6) in mice's serum while up-regulating the level of anti-inflammatory factor (IL-10) during the healing process. Furthermore, SCP suppressed the NF-κB pathway by decreasing protein levels of phosphorylated p65 and IKKα, and increasing protein levels of IκBα.

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