Xiaoyan Wang, Jinghe Sun, Ke Liu, Shuang Li, Jun Zhao, Jingfeng Yang
{"title":"海参蛋白膏对小鼠皮肤创面愈合的影响及其潜在的抗炎机制。","authors":"Xiaoyan Wang, Jinghe Sun, Ke Liu, Shuang Li, Jun Zhao, Jingfeng Yang","doi":"10.1088/1748-605X/addcaa","DOIUrl":null,"url":null,"abstract":"<p><p>Natural substances with anti-inflammatory activity have always been the priority for human injuries. This study aims to investigate the beneficial effects and mechanism of sea cucumber protein (SCP) on wound healing, through a BALB/c mouse model and lipopolysaccharides-induced RAW 264.7 cells. We identified the mice's serum cytokines and tissue section to find out how SCP paste works. The alteration of the nuclear factor-<i>κ</i>B (NF-<i>κ</i>B) pathway during the anti-inflammatory effect of SCP was also explored. The results showed that the wound healing rate in the SCP(H) group exceeded 90%, whereas it was 72.91% and 64.10% in the Control and negative control groups on day 14. New blood vessels and fibroblasts were generated in the wounds. Collagen expression increased by 13.89% and 15.12% respectively in the SCP(L) and SCP(H) groups compared with the Control group on day 14. Furthermore, SCP decreased the levels of pro-inflammatory factors (tumor necrosis factor-<i>α</i>, interleukin (IL)-1<i>β</i>, IL-6) in mice's serum while up-regulating the level of anti-inflammatory factor (IL-10) during the healing process. Furthermore, SCP suppressed the NF-<i>κ</i>B pathway by decreasing protein levels of phosphorylated p65 and IKK<i>α</i>, and increasing protein levels of I<i>κ</i>B<i>α</i>.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of sea cucumber protein paste for mice's skin wound healing and its potential anti-inflammatory mechanism.\",\"authors\":\"Xiaoyan Wang, Jinghe Sun, Ke Liu, Shuang Li, Jun Zhao, Jingfeng Yang\",\"doi\":\"10.1088/1748-605X/addcaa\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Natural substances with anti-inflammatory activity have always been the priority for human injuries. This study aims to investigate the beneficial effects and mechanism of sea cucumber protein (SCP) on wound healing, through a BALB/c mouse model and lipopolysaccharides-induced RAW 264.7 cells. We identified the mice's serum cytokines and tissue section to find out how SCP paste works. The alteration of the nuclear factor-<i>κ</i>B (NF-<i>κ</i>B) pathway during the anti-inflammatory effect of SCP was also explored. The results showed that the wound healing rate in the SCP(H) group exceeded 90%, whereas it was 72.91% and 64.10% in the Control and negative control groups on day 14. New blood vessels and fibroblasts were generated in the wounds. Collagen expression increased by 13.89% and 15.12% respectively in the SCP(L) and SCP(H) groups compared with the Control group on day 14. Furthermore, SCP decreased the levels of pro-inflammatory factors (tumor necrosis factor-<i>α</i>, interleukin (IL)-1<i>β</i>, IL-6) in mice's serum while up-regulating the level of anti-inflammatory factor (IL-10) during the healing process. Furthermore, SCP suppressed the NF-<i>κ</i>B pathway by decreasing protein levels of phosphorylated p65 and IKK<i>α</i>, and increasing protein levels of I<i>κ</i>B<i>α</i>.</p>\",\"PeriodicalId\":72389,\"journal\":{\"name\":\"Biomedical materials (Bristol, England)\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-06-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical materials (Bristol, England)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1088/1748-605X/addcaa\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical materials (Bristol, England)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1088/1748-605X/addcaa","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of sea cucumber protein paste for mice's skin wound healing and its potential anti-inflammatory mechanism.
Natural substances with anti-inflammatory activity have always been the priority for human injuries. This study aims to investigate the beneficial effects and mechanism of sea cucumber protein (SCP) on wound healing, through a BALB/c mouse model and lipopolysaccharides-induced RAW 264.7 cells. We identified the mice's serum cytokines and tissue section to find out how SCP paste works. The alteration of the nuclear factor-κB (NF-κB) pathway during the anti-inflammatory effect of SCP was also explored. The results showed that the wound healing rate in the SCP(H) group exceeded 90%, whereas it was 72.91% and 64.10% in the Control and negative control groups on day 14. New blood vessels and fibroblasts were generated in the wounds. Collagen expression increased by 13.89% and 15.12% respectively in the SCP(L) and SCP(H) groups compared with the Control group on day 14. Furthermore, SCP decreased the levels of pro-inflammatory factors (tumor necrosis factor-α, interleukin (IL)-1β, IL-6) in mice's serum while up-regulating the level of anti-inflammatory factor (IL-10) during the healing process. Furthermore, SCP suppressed the NF-κB pathway by decreasing protein levels of phosphorylated p65 and IKKα, and increasing protein levels of IκBα.