Biomedical materials (Bristol, England)最新文献

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Degradation, swelling, and drug release behavior of injectable ETTMP/PEGDA hydrogels. 注射用ETTMP/PEGDA水凝胶的降解、肿胀和药物释放行为。
Biomedical materials (Bristol, England) Pub Date : 2025-04-07 DOI: 10.1088/1748-605X/adc21e
Paige N Rockwell, Erin L Jablonski, Brandon M Vogel
{"title":"Degradation, swelling, and drug release behavior of injectable ETTMP/PEGDA hydrogels.","authors":"Paige N Rockwell, Erin L Jablonski, Brandon M Vogel","doi":"10.1088/1748-605X/adc21e","DOIUrl":"10.1088/1748-605X/adc21e","url":null,"abstract":"<p><p>The erosion and drug release behavior of an injectable hydrogel composed of ethoxylated trimethylolpropane tri-3-mercaptopropionate (ETTMP) and poly(ethylene glycol) diacrylate were determined under physiological conditions. Water and polymer mass changes were monitored over time to characterize the swelling/deswelling and erosion of the hydrogel tablets. Experimental data were collected for hydrogels with varying polymer fractions. These data were used to develop an empirical model to predict the eroding mass change and equilibrium water content across different compositions. Three easily detectable model drugs (methylene blue (MB), sulforhodamine 101, and chloroquine) were loaded into 25, 35, and 50 wt% polymer hydrogels to understand their drug release behavior. The gelation time and time for total drug release were dependent on the weight fraction of the polymer in the hydrogel and varied with the pH of the drug solutions, with more acidic drugs increasing gelation time. Complete drug release was not observed for MB because of the reaction with ETTMP thiol groups, demonstrating the importance of understanding the potential interactions between the drug and polymer. Drug-loaded hydrogels were also monitored for erosion and were found to swell more than their neat counterparts for all drugs tested, suggesting an effect of drug loading on the extent of hydrogel crosslinking.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of PLGA microspheres loaded with natural drug tannins in tumor treatment. 载入天然药物单宁酸的聚乳酸乙二醛(PLGA)微球在肿瘤治疗中的应用。
Biomedical materials (Bristol, England) Pub Date : 2025-04-04 DOI: 10.1088/1748-605X/adc6d7
Yiqiao Zhao, Yixuan Huang, Chaofan Chen, Chaoyu Cai, Xin Liu, Xiaohong Jiang, Aliaksandr Rahachou
{"title":"Application of PLGA microspheres loaded with natural drug tannins in tumor treatment.","authors":"Yiqiao Zhao, Yixuan Huang, Chaofan Chen, Chaoyu Cai, Xin Liu, Xiaohong Jiang, Aliaksandr Rahachou","doi":"10.1088/1748-605X/adc6d7","DOIUrl":"10.1088/1748-605X/adc6d7","url":null,"abstract":"<p><p>Although conventional chemotherapeutic drugs exhibit broad-spectrum antitumor efficacy, their toxic effects on normal cells cannot be ignored, and there is an urgent need to develop novel drugs to improve therapeutic efficacy and reduce side effects. In this study, we prepared PLGA/TA microspheres with TA as the target drug and PLGA as the carrier, and evaluated their physicochemical properties, including particle size, morphology, and release behavior, with the aim of exploring the potential of PLGA/TA microspheres for tumor therapy. The results of RNA sequencing showed that PLGA/TA microspheres may act on tumor cells mainly through the pathway of PI3K-Akt, and do not interfere with the synthesis of DNA directly. Overall, PLGA/TA microspheres, as a novel drug delivery system, showed good anti-tumor potential and provided new ideas and directions for cancer therapy.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of magnesium-based components for intraocular drug delivery byin vitrobiocompatibility and drug-device interaction. 通过体外生物相容性和药物装置相互作用评估眼内给药镁基组分。
Biomedical materials (Bristol, England) Pub Date : 2025-04-03 DOI: 10.1088/1748-605X/adc21f
Marco Ferroni, Francesco De Gaetano, Manuela Zonfrillo, Nina Bono, Matteo Giuseppe Cereda, Pasquale Pierimarchi, Gianluca Sferrazza, Gabriele Candiani, Federica Boschetti
{"title":"Assessment of magnesium-based components for intraocular drug delivery by<i>in vitro</i>biocompatibility and drug-device interaction.","authors":"Marco Ferroni, Francesco De Gaetano, Manuela Zonfrillo, Nina Bono, Matteo Giuseppe Cereda, Pasquale Pierimarchi, Gianluca Sferrazza, Gabriele Candiani, Federica Boschetti","doi":"10.1088/1748-605X/adc21f","DOIUrl":"10.1088/1748-605X/adc21f","url":null,"abstract":"<p><p>The development of magnesium-based intraocular drug delivery devices holds significant promise for biomedical applications, particularly in treating wet age-related macular degeneration (AMD) using vascular endothelial growth factor inhibitors such as bevacizumab. Magnesium's rapid degradation, which can be finely tuned to achieve the controlled release required for AMD treatment, along with its well-established biocompatibility and biodegradable properties, positioning it as an ideal material for these applications. The study aimed to evaluate magnesium's potential as a carrier for ocular drug delivery systems by demontrating the stability of monoclonal antibodies, specifically bevacizumab, in the presence of magnesium corrosion products and the biocompatibility of these products with various cell lines, including murine fibroblasts (3T3), rat retinal Müller cells, and human retinal pigment epithelial cells (ARPE19). The stability of bevacizumab with pure magnesium (Mg) was investigated through an indirect enzyme-linked immunosorbent assay protocol, developed and customized for this specific aim. The biocompatibility of Mg corrosion products was assessed by toxicological evaluations through MTT and Trypan Blue Viability assays, along with cell cycle analysis. Results demonstrated no significant impact of Mg corrosion products on bevacizumab stability, with changes in mean values consistently below or equal to 10%. Furthermore, Mg extracts showed minimal cytotoxicity, as metabolic activity exceeded 80% across all cell lines, classified as Grade 0/1 cytotoxicity under ISO 10993-5 standards. Cell viability, proliferation, and morphology remained unaffected for up to 72 h of exposure. This study provides the first<i>in vitro</i>evaluation of bevacizumab's stability in the presence of magnesium corrosion products and its biocompatibility with retinal cell lines, laying the foundation for future ophthalmic research and underscoring magnesium's potential as a material for intraocular drug delivery systems.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin delivery system based on a chitosan-liposome encapsulated zeolitic imidazolate framework-8: a potential treatment antioxidant and antibacterial treatment after phacoemulsification. 基于壳聚糖-脂质体包裹沸石咪唑酸框架-8的姜黄素输送系统:一种潜在的乳化术后抗氧化和抗菌治疗方法。
Biomedical materials (Bristol, England) Pub Date : 2025-04-03 DOI: 10.1088/1748-605X/adc05c
Meiting Lin, Kunyuan Tang, Wendi Zheng, Shijie Zheng, Ke Hu
{"title":"Curcumin delivery system based on a chitosan-liposome encapsulated zeolitic imidazolate framework-8: a potential treatment antioxidant and antibacterial treatment after phacoemulsification.","authors":"Meiting Lin, Kunyuan Tang, Wendi Zheng, Shijie Zheng, Ke Hu","doi":"10.1088/1748-605X/adc05c","DOIUrl":"10.1088/1748-605X/adc05c","url":null,"abstract":"<p><p>Curcumin is a natural polyphenol extracted from plants that can interact with various molecular targets, including antioxidant, antibacterial, anticancer, and anti-aging activities. Due to its variety of pharmacological activities and large margin pf safety, curcumin has been used in the prevention and treatment of various diseases, such as Alzheimer's, heart, and rheumatic immune diseases. To develop curcumin eye drops that can be used as antioxidant and antibacterial agents after phacoemulsification, we have designed a nano-based drug delivery system to improve curcumin bioavailability and duration of action. We successfully prepared zeolitic imidazolate framework-8 (ZIF-8) coated with chitosan-liposome (Cur@ZIF-8/CS-Lip) for curcumin delivery. It can release curcumin for over 20 h<i>in vitro</i>and exhibits excellent biosafety, antioxidant, and antibacterial activities. Therefore, we hypothesized that Cur@ZIF-8/CS-Lip could reduce the incidence of oxidative stress and infection after cataract surgery.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic interactions between physical exercise intervention, innovative materials, and neurovascular coupling in bone repair and injury recovery: a comprehensive review. 体育锻炼干预、创新材料和神经血管耦合在骨修复和损伤恢复中的协同相互作用:综合综述。
Biomedical materials (Bristol, England) Pub Date : 2025-04-03 DOI: 10.1088/1748-605X/adc5c0
Jiejie Guo, Ting Zhang, Mengjia Li, Qinwen Wang, Xianting Ding
{"title":"Synergistic interactions between physical exercise intervention, innovative materials, and neurovascular coupling in bone repair and injury recovery: a comprehensive review.","authors":"Jiejie Guo, Ting Zhang, Mengjia Li, Qinwen Wang, Xianting Ding","doi":"10.1088/1748-605X/adc5c0","DOIUrl":"10.1088/1748-605X/adc5c0","url":null,"abstract":"<p><p>Bone injury presents a prevalent challenge in clinical settings, with traditional treatment modalities exhibiting inherent limitations. Recent advancements have highlighted the potential of combining physical exercise intervention and innovative materials to enhance bone repair and recovery. This review explores the synergistic effects of physical exercise and novel materials in promoting bone regeneration, with a particular focus on the role of neurovascular coupling (NVC) mechanisms. Physical exercise not only stimulates bone cell function and blood circulation but also enhances the bioactivity of novel materials, such as nanofiber membranes and smart materials, which provide supportive scaffolds for bone cell attachment, proliferation, and differentiation. NVC, involving the interaction between neural activity and blood flow, is integral to the bone repair process, ensuring the supply of nutrients and oxygen to the injured site. Studies demonstrate that the combination of physical exercise and novel materials can accelerate bone tissue regeneration, with exercise potentially enhancing the bioactivity of materials and materials improving the effectiveness of exercise. However, challenges remain in clinical applications, including patient variability, material biocompatibility, and long-term stability. Optimizing the integration of physical exercise and novel materials for optimal therapeutic outcomes is a key focus for future research. This review examines the collaborative mechanisms between physical exercise, novel materials, and NVC, emphasizing their potential and the ongoing challenges in clinical settings. Further exploration is needed to refine their application and improve bone repair strategies.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143733425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytotoxicity mitigated silica entrapped copper doped Zinc Sulfide Quantum dots as luminescent nanoprobes for biolabeling. 细胞毒性减弱的二氧化硅包覆铜掺杂硫化锌量子点作为发光纳米探针用于生物标记。
Biomedical materials (Bristol, England) Pub Date : 2025-04-01 DOI: 10.1088/1748-605X/adc230
Remya Muralimanohar, S Saravana Kumar
{"title":"Cytotoxicity mitigated silica entrapped copper doped Zinc Sulfide Quantum dots as luminescent nanoprobes for biolabeling.","authors":"Remya Muralimanohar, S Saravana Kumar","doi":"10.1088/1748-605X/adc230","DOIUrl":"10.1088/1748-605X/adc230","url":null,"abstract":"<p><p>Quantum dots (QDs) are luminescent semiconductor nanoparticles with unique optical properties that facilitate their use in sensing, biological labeling, optical imaging, and diagnostics. Wider band gap materials, such as Zinc sulfide, are extensively employed as QD nanoprobes since they offer higher photostability, higher quantum yield, larger molar extinction coefficients, and longer fluorescence lifetimes than conventional organic fluorescent dyes used in bioassays. Tunable multiphoton emission in QDs is accomplished by doping with transition metals, of which, copper is the most beneficial owing to its comparable ionic radius, intense emission, and composition-variable spectral broadening. However, an overdose of Cu is toxic to the cells, leading to apoptosis. This cytotoxicity impedes the utilization of Cu-doped ZnS QDs for biolabeling. The present work deals with the diminution of copper cytotoxicity in Cu-doped ZnS Q-dots by means of silica entrapment, equipping them for<i>in vitro</i>and<i>in vivo</i>bioassays in the future. Cu-doped ZnS Q-dots were synthesized by chemical precipitation method and overlaid with silica by sol-gel method. Cytotoxicity investigation was performed on L929 Mouse fibroblast cells. X-ray diffraction studies confirmed that the prepared Q-dots were approximately 2 nm in size and were in the cubic phase. High resolution transmission electron microscopy revealed the spherical morphology of Q-dots. Micro-Raman Analysis was used to determine the Raman modes of the samples. Band gap energy was computed using UV-Visible Spectroscopy. Photoluminescence (PL) Spectroscopy demonstrated two emission peaks around 418 nm and 455 nm due to sulfur vacancy and copper trap levels, respectively, for Cu:ZnS Q-dots with hiked PL intensity on silica coating.<i>In vitro</i>cell toxicity studies performed on the as-prepared Q-dots by microscopic observation of treated cells, as well as by MTT colorimetric assay, manifested the attenuation of cytotoxicity in silica overspread copper-doped Q-dots. Silica entrapment subsided the copper-induced cytotoxicity by minimizing the photochemical oxidation of the Q-dots surface together with making them hydrophilic. Furthermore, silica coating boosted the PL intensity of the Q-dots. Such Q-dots could be a potent alternative to fluorescent organic pigments for biolabeling.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Floating or adherent hepatocyte spheroid cultures using microwell chips with polyethylene glycol or polyimide surfaces. 漂浮或贴壁肝细胞球形培养使用微孔芯片与聚乙二醇或聚酰亚胺表面。
Biomedical materials (Bristol, England) Pub Date : 2025-03-31 DOI: 10.1088/1748-605X/adc17d
Sae Yokomine, Tomomi Makino, Emiko Nagao, Kohji Nakazawa
{"title":"Floating or adherent hepatocyte spheroid cultures using microwell chips with polyethylene glycol or polyimide surfaces.","authors":"Sae Yokomine, Tomomi Makino, Emiko Nagao, Kohji Nakazawa","doi":"10.1088/1748-605X/adc17d","DOIUrl":"10.1088/1748-605X/adc17d","url":null,"abstract":"<p><p>Microwell chip culture is a promising technique for controlling spheroid size and producing a large number of homogeneous spheroids. In this study, we focused on the relationship between chip material and the properties of hepatocyte spheroids. The basic chip design was 397 circular microwells, each 400 µm in diameter. Two types of microwell chips were fabricated, coating the bottom surface either with polyethylene glycol (PEG chip) or polyimide (PI chip). Hepatocytes gradually aggregated and formed floating spheroids within each microwell in the PEG chip but formed adherent spheroids within each microwell of the PI chip. Such floating and adherent spheroid morphologies were maintained for at least one month of culture. An explanation for the spheroid formation mechanism is that the plasminogen activator (PA) /plasmin and matrix degradation/remodeling systems were activated in the formation of adherent spheroids. Furthermore, in adherent spheroid cultures, the formation of cell-matrix junctions was promoted, in addition to the development of intercellular junctions. The albumin secretion and drug metabolism activities of the hepatocyte spheroids were higher than those of traditional monolayer hepatocytes, and the adherent spheroids in the PI chip maintained a higher functional expression than the floating spheroids in the PEG chip. Further to this, functional properties of hepatocytes, the expressions of key metabolic enzymes, glucose 6-phosphatase (sugar metabolism), tryptophan 2, 3-dioxygenase (amino acid metabolism), arginase 1 (urea cycle), cytochrome P450 7a1 (lipid metabolism), and cytochrome P450 families (drug metabolism) were evaluated by gene expression analysis. The expression of these key enzymes in hepatocytes was higher in spheroid culture than in general monolayer culture, and the functions of adherent spheroids were superior to those of floating spheroids. These results indicate that the material properties of the microwell chips are important factors that regulate the morphological and functional characteristics of hepatocyte spheroids.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143652343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Personalized assessment and monitoring of bone health from sweat: unveiling TEGO doped wearable, non-invasive hydrogel nanocomposite biosensor empowered by IL-6 detection. 汗液中骨骼健康的个性化评估和监测:揭示了由IL-6检测授权的TEGO掺杂可穿戴、非侵入性水凝胶纳米复合生物传感器。
Biomedical materials (Bristol, England) Pub Date : 2025-03-31 DOI: 10.1088/1748-605X/adc05a
Seema Rani, Sanchita Bandyopadhyay-Ghosh, Subrata Bandhu Ghosh
{"title":"Personalized assessment and monitoring of bone health from sweat: unveiling TEGO doped wearable, non-invasive hydrogel nanocomposite biosensor empowered by IL-6 detection.","authors":"Seema Rani, Sanchita Bandyopadhyay-Ghosh, Subrata Bandhu Ghosh","doi":"10.1088/1748-605X/adc05a","DOIUrl":"10.1088/1748-605X/adc05a","url":null,"abstract":"<p><p>Portable biosensing is crucial for rapid detection and continuous monitoring of bone diseases such as osteoporosis and bone cancer. It is well established that such bone disorders or diseases trigger release of inflammatory cytokines including interleukin-6 (IL6), detectable in sweat by electrochemical immunosensors. To this end, this study presents a novel hydrogel nanocomposite based immunosensor with highly conductive dual-layer of thermally exfoliated graphene oxide, toward precise detection and determination of loading level of IL-6 biomarker, and in turn, developing a label-free flexible bone biosensing platform. The immunosensor employed antibody immobilization process, which was further facilitated by the modification of the dual-layer by using 1-pyrenebutyric acid N-hydroxy succinimide ester. A thorough analysis of the effects of surface modification was conducted utilizing spectroscopic, electrochemical, and morphological methods. The biosensor's response was assessed through the utilization of the cyclic voltammetry measurement, which exhibited remarkable selectivity, achieving a low limit of detection of 15.4 pg ml<sup>-1</sup>across a wide linear range. Additionally, field emission scanning electron microscopy, Fourier transform infrared spectroscopy and Raman spectroscopy were successfully used to validate the sensing substrate in bio-fluidic samples and to understand the structure-property correlation. This innovative portable and flexible biosensor thus offers a practical and effective tool for potential application in continuous monitoring of bone health.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143627007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Osteogenic function of BMP2-modified PEEK scaffolds for orbital fracture repair. bmp2修饰PEEK支架眶骨折修复的成骨功能。
Biomedical materials (Bristol, England) Pub Date : 2025-03-27 DOI: 10.1088/1748-605X/adc220
Yujie Wu, Cuihong Liu, Jinhua Liu, Wenwen Wang, Bixuan Qin, Honglei Liu
{"title":"Osteogenic function of BMP2-modified PEEK scaffolds for orbital fracture repair.","authors":"Yujie Wu, Cuihong Liu, Jinhua Liu, Wenwen Wang, Bixuan Qin, Honglei Liu","doi":"10.1088/1748-605X/adc220","DOIUrl":"10.1088/1748-605X/adc220","url":null,"abstract":"<p><p>This study aimed to investigate the osteogenic function of polyetheretherketone (PEEK) scaffolds modified with bone morphogenetic protein 2 (BMP2) and its possibility for orbital fracture repair. The 3D-printed PEEK sheets were combined with BMP2-loaded hyaluronic acid hydrogel (HAH) to fabricate PEEK-BMP2-HAH composite scaffolds. Bone marrow mesenchymal stem cells (BMSCs) were seeded onto PEEK or PEEK-BMP2-HAH scaffolds. Cell adhesion and cell proliferation were measured by transmission electron microscopy and CCK-8 assay. Alkaline phosphatase (ALP) chromogenic, alizarine red S staining, and PCR analysis of Runt-related transcription factor 2 (Runx2), collagen-I (Col-I), Osterix, and osteopontin (OPN) were performed to assess osteogenic activity. The rat orbital fracture defect model is proposed for evaluating the biocompatibility, osteogenic integration, and functional recovery of PEEK orbital implants. Compared with PEEK, cell adhesion and cell proliferation were increased in PEEK-BMP2-HAH scaffolds. ALP activity and mineralized nodule formation were increased in PEEK-BMP2-HAH scaffolds than that in PEEK the mRNA expression of Runx2, Osterix, Col-I and OPN was increased on PEEK-BMP2-HAH scaffolds than that on PEEK at 14 d of osteogenic induction. Besides, a bone defect animal model revealed that BMP2-HAH-modified PEEK scaffolds could effectively facilitate the repair of the orbital bone defect, with increased expression of OPN and Runx2. BMP2-loaded HAH effectively increased adhesion, proliferation, and osteogenic differentiation of BMSCs on PEEK. PEEK-BMP2-HAH scaffolds are expected to become new materials for orbital fracture repair.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biocompatible nanozyme with dual catalytic activities for high-performance multimodality therapy against glioblastoma. 具有双重催化活性的生物相容性纳米酶,用于针对胶质母细胞瘤的高效多模式疗法。
Biomedical materials (Bristol, England) Pub Date : 2025-03-26 DOI: 10.1088/1748-605X/adc05b
Guihong Lu, Xiaoyan Li, Wenfei Xu, Fan Zhang, Xiang Chen, Huibin Wu, Haibing Dai, Feng Li, Weidong Nie
{"title":"Biocompatible nanozyme with dual catalytic activities for high-performance multimodality therapy against glioblastoma.","authors":"Guihong Lu, Xiaoyan Li, Wenfei Xu, Fan Zhang, Xiang Chen, Huibin Wu, Haibing Dai, Feng Li, Weidong Nie","doi":"10.1088/1748-605X/adc05b","DOIUrl":"10.1088/1748-605X/adc05b","url":null,"abstract":"<p><p>Nanozymes based on metals have been regarded as a promising candidate in the metabolic reprogramming of low-survival, refractory glioblastoma multiforme (GBM). However, due to size limitations, nanozymes struggle to balance catalytic activity with the ability to cross the blood-brain barrier (BBB), limiting their efficiency in GBM therapy. Herein, we establish a hybrid nanocluster, AuMn NCs, by cross-linking ultrasmall nano-gold (Au) and manganese oxide (MnO<sub>2</sub>), which overcomes the size requirement conflict for integrating catalytic activities, long-period circulation, photothermal effect, glucose consumption, and chemodynamic effect for multimodality treatment against GBM. After administered intravenously, the overall large-size AuMn NCs can escape kidney filtration and cross the BBB for GBM accumulation. Then the individual ultrasmall nano-MnO<sub>2</sub>components effectively catalyze H<sub>2</sub>O<sub>2</sub>degradation as catalase to produce oxygen, which is utilized by individual ultrasmall nano-Au components to consume glucose as glucose oxidase for starvation therapy. The H<sub>2</sub>O<sub>2</sub>generated during Au-catalyzed glucose consumption further facilitates MnO<sub>2</sub>catalytic activity. Such positive feedback overwhelmingly intervenes in the glucose metabolism of GBM. Concurrently, clustered Au-induced photothermal effect and released Mn<sup>2+</sup>-induced chemodynamic effect further contribute to eliminating GBM cells. The versatile clustered nanozyme offers a feasible strategy for the multimodality intervention of GBM.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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