Biomedical materials (Bristol, England)最新文献

{"title":"Utilizing biological responses to evaluate the<i>in vivo</i>fate and biocompatibility of reduced graphene oxide-integrated nerve guidance conduits for peripheral nerve repair.","authors":"Jingyun Xi, Xiaojing Li, Qi Guo, Guohang Wang, Lifang Jia, Liming Xie, Meiyu Wu, Dezhen Song, Siyuan Wang, Bingbing Sun, Ying Liu, Tun Yuan","doi":"10.1088/1748-605X/ae8a2b","DOIUrl":"10.1088/1748-605X/ae8a2b","url":null,"abstract":"<p><p>Reduced graphene oxide (rGO) has been widely incorporated into nerve guidance conduits (NGCs) to promote peripheral nerve regeneration owing to its physicochemical properties. However, some studies have reported potential biocompatibility risks of rGO and raised concerns about its possible adverse effects on nerve regeneration. In this study, an<i>in vivo</i>challenge model was established. Optical microscopy techniques were comprehensively employed to track the distribution and degradation behavior of rGO after implantation of rGO-incorporated NGCs, and biological evidence at both cellular and tissue levels was integrated to support the degradation-compatibility evaluation of rGO. Histological observations revealed the presence of rGO fragments and the formation of fibrous capsule walls. Immunofluorescence staining showed that rGO did not persistently interfere with the expression of nerve repair-related proteins up to 26 weeks post-implantation. RNA-sequencing confirmed inflammation at the implantation site within 4 weeks, as well as enrichment of differentially expressed genes in categories associated with nerve repair and cellular energy metabolism. These findings indicate that, within the framework of this model, the material exhibits biosafety during nerve repair and regeneration, although non-transient biocompatibility risks still warrant careful consideration. This study provides a theoretical support and experimental reference for improving the biocompatibility evaluation of graphene oxide derivatives.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148439109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in nano-delivery of natural products for cancer therapy: mechanisms and translational strategies.","authors":"Xiang Chen, Huibin Wu, Xinyu Pan, Weiqun Li, Haibing Dai, Han Wang, Jie Mao, Jianbo Yu","doi":"10.1088/1748-605X/ae7ee2","DOIUrl":"10.1088/1748-605X/ae7ee2","url":null,"abstract":"<p><p>Cancer remains a major global health burden, with conventional monotherapies often limited by recurrence, therapeutic resistance, and systemic toxicity. Natural products, derived from diverse sources and characterized by distinct chemical structures, exhibit broad anticancer potential by modulating multiple molecular pathways. However, their clinical application is frequently constrained by poor solubility, low bioavailability, and chemical instability. Nano-delivery systems (NDS) have emerged as a promising strategy to address these limitations by improving pharmacokinetic properties, stabilizing labile compounds, and enabling co-delivery with conventional therapeutics, thereby supporting multimodal and synergistic interventions. This review provides a comprehensive overview of natural product-based NDS in cancer therapy, beginning with classification strategies and mechanistic frameworks, followed by an analysis of how NDS enhance pharmacological performance and facilitate integration into combination regimens. Finally, it highlights key translational challenges and opportunities, offering a foundation for the future development and clinical translation of natural product-based NDS.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148273741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive Uio-66 coating on polydopamine-modified titanium implants.","authors":"Aslı Ece Gümüşkaya, Berna Topuz, Sezin Galioglu, Ayşe Karakeçili","doi":"10.1088/1748-605X/ae8c9d","DOIUrl":"10.1088/1748-605X/ae8c9d","url":null,"abstract":"<p><p>Engineering the surface of titanium (Ti) with bioactive coatings is an effective approach to address biofunctional limitations of Ti implants, including low bioactivity and poor tissue integration. Here, Ti substrates were functionalized with the UiO-66 metal-organic framework (MOF) via a polydopamine-assisted coating strategy to enhance cell-Ti implant interactions. The colloidal coating results in the formation of well-controlled surface with moderate hydrophilicity (water contact angle<b>∼</b>70°) and a reduced local surface roughness value (Ra<b>∼</b>23 nm) relative to<i>in situ</i>growth (Ra<b>∼</b>76 nm). The optimized coating strategy developed in this study reveals that MOF nanocrystal loading, surface roughness, hydrophilicity and wettability critically determine the biointerface behavior and overall coating performance. The resulting hybrid morphology further promotes MC3T3-E1 cell viability on the implant surface while maintaining structural stability.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-density microwell arrays enable controlled pseudoislet engineering for diabetes cell therapy.","authors":"Charline Rosenberger, Carolin Heller, Tomasz M Basiewicz, Susann Lehmann, Victoria Sarangova, Hany Abdelgawad, Undine Schubert, Janine Schmid, Carsten Werner, Stefan R Bornstein, Petra B Welzel, Barbara Ludwig","doi":"10.1088/1748-605X/ae88a4","DOIUrl":"10.1088/1748-605X/ae88a4","url":null,"abstract":"<p><p>Macroencapsulation systems for pancreatic islet delivery are an emerging strategy for cell replacement therapy in type 1 diabetes, aimed at eliminating systemic immunosuppression and enabling the use of alternative cell sources. A critical design challenge is to achieve high packing densities while maintaining sufficient oxygen and nutrient supply to ensure islet viability and function. This design-focused study presents a one-step method for the simultaneous and defined formation and spatial arrangement of uniformly sized pancreatic pseudoislets using high-density concave microwell arrays with potential for future integration into macroencapsulation devices. Microwells with diameters between 130 and 200 µm were arranged in a hexagonal pattern with inter-well spacings of 50 or 100 µm to maximize the packing density while maintaining separation between clusters. The effects of microwell depth, diameter, and inter-well spacing on pseudoislet size, morphology, viability, and glucose-stimulated insulin secretion were systematically evaluated using reaggregated rat islet cells as a standardized and well-characterized model system under normoxic and hypoxic conditions. The optimized microwell configuration enabled efficient pseudoislet formation with a tailorable, uniform size, high viability, and preserved insulin secretory function, even at high densities. This scalable microwell array technology addresses key limitations of current pseudoislet culture and transplantation methods, thus providing a base for improved macroencapsulation device design in diabetes cell therapy.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148426877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of type H vessels in bone repair of rat tibial defects treated with stromal vascular fraction-collagen sponge composites.","authors":"Xieyang Mei, Chenyang Xu, Yawen Xu, Han Sun, Kaiming Luo, Shoukui Xiang, Fei Hua","doi":"10.1088/1748-605X/ae834d","DOIUrl":"10.1088/1748-605X/ae834d","url":null,"abstract":"<p><p>To address the clinical limitations of autografts and allografts in bone defect repair, this study aimed to develop a novel composite graft by combining adipose-derived stromal vascular fraction (SVF) with medical-grade collagen sponge, aiming to achieve structural support and functional vascularization simultaneously. A rat tibial defect model was established, and the SVF was combined with medical collagen sponge to form a composite for implantation.<i>In vitro</i>experiments were conducted to detect the proliferation activity, osteogenic differentiation ability, and pro-angiogenic potential of SVF cells.<i>In vivo</i>experiments were used to evaluate the bone volume fraction (BV/TV) and bone mineral density (BMD) in the bone defect area and observe the formation of CD31hi/Emcnhi vessels (H-type vessels). Immunofluorescence staining was performed to analyze the expression of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF).<i>In vitro</i>SVF exhibited robust proliferation, osteogenic differentiation, and secreted factors that significantly enhanced endothelial cell migration and tube formation.<i>In vivo</i>, the SVF-collagen sponge composite significantly increased the bone volume fraction (BV/TV) and BMD at 9 weeks post-operation compared to controls. Importantly, at this 9 week time point, the SVF composite group demonstrated a greater abundance of type H vessels within the defect compared to both the scaffold-only and blank control groups. This was accompanied by sustained upregulation of VEGF and PDGF expression. The SVF-collagen sponge composite promotes bone regeneration in a rat defect model. The mechanism is associated with fostering a pro-angiogenic microenvironment, characterized by increased type H vessel formation and elevated expression of VEGF and PDGF, suggesting enhanced angiogenesis-osteogenesis coupling. This autologously-sourced composite shows promising translational potential due to its straightforward preparation and biocompatibility.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148341250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-functional porous PLA microspheres: an<i>in-vitro</i>study of a platform for plasma-rich in growth factors (PRGF) delivery.","authors":"Mohammadhossein Shah-Mohammadi-Loshani, Rana Imani, Mahdi Khalili","doi":"10.1088/1748-605X/ae8c9c","DOIUrl":"10.1088/1748-605X/ae8c9c","url":null,"abstract":"<p><p>Effective management of acute and chronic wounds remains a major clinical challenge, driving the need for innovative therapeutic approaches. Plasma-rich in growth factors (PRGF), an autologous and bioactive fraction of platelet-rich plasma, has emerged as a promising agent for stimulating tissue repair and angiogenesis. In this study, poly (lactic acid) (PLA) microparticles loaded with PRGF were developed via a double emulsion (water-in-oil-in-water, W/O/W) solvent evaporation technique. Processing parameters, including synthesis and drying conditions, were optimized to yield a porous microparticle architecture conducive to controlled release. Morphological analysis using scanning electron microscopy confirmed the formation of uniformly spherical microparticles (PLMs) with an average diameter of 26.51 ± 7.86 µm and a mean pore size of 2.79 ± 0.55 µm.<i>In-vitro</i>release profiles demonstrated sustained PRGF release over a period of more than 17 d. Additionally, cell-based assays revealed that the PRGF-loaded PLMs effectively supported the proliferation, adhesion, and migration of human dermal fibroblasts. These results highlight the potential of PRGF-loaded PLA microparticles as a biocompatible and long-acting platform for advanced wound healing and regenerative medicine applications.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148474433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing dual gradients of topography and mineralization via a modified electrospinning strategy in polyacrylonitrile/MWCNT nanofibrous membranes for enhanced osteogenic differentiation.","authors":"Subin Park, Jeong In Kim, Soonchul Lee","doi":"10.1088/1748-605X/ae86a4","DOIUrl":"10.1088/1748-605X/ae86a4","url":null,"abstract":"<p><p>The rational design of bone-regenerative scaffolds requires precise control over nano- to microscale architecture and biomineralization kinetics. Here, we report a modified electrospinning strategy to fabricate polyacrylonitrile MWCNT nanofibrous scaffolds with a gradient topography ranging from aligned to random orientations. This unique architecture harnesses anisotropy to accelerate HAp mineralization, achieving spatially controlled, gradient HAp deposition across the scaffold. Incorporation of MWCNTs not only enhanced the conductivity and nucleation sites for HAp but also synergized with the gradient structure to promote rapid and uniform biomineralization. Upon Human mesenchymal stem cell seeding, distinct osteogenic outcomes emerged depending on both MWCNT incorporation and scaffold topography, whereby variations in hydroxyapatite mineralization along the gradient framework translated into differential bone-like matrix formation. These findings reveal how topographical gradients and conductive nanocomposites act in concert to orchestrate cell-material interactions, guiding osteogenesis through spatially defined biomineral cues. Collectively, this study establishes a bioinspired and potentially translatable platform for next-generation bone regeneration therapies and highlights the broader promise of gradient-engineered nanofiber systems in regenerative medicine.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on assessment of advances in polymers and their hybrid nanosystem for leukaemia theranostics applications.","authors":"Soni Prajapati, Akash Kumar, Ranjana Singh","doi":"10.1088/1748-605X/ae83f7","DOIUrl":"10.1088/1748-605X/ae83f7","url":null,"abstract":"<p><p>Leukaemia, a widespread haematological cancer, possesses particular challenges in theranostics despite the availability of traditional and modern approaches. This article addresses a significant gap in the literature: a lack of focus on polymeric and hybrid nanoparticles (P&HNP) in advancing leukaemia therapy. It explores how to overcome limitations of conventional therapeutic approaches. The rationale for this study stems from several considerations, including the fact that while polymeric nanoparticles show promise in leukaemia theranostics, a comprehensive assessment of how biomaterial choices influence therapeutic efficacy remains lacking. This article briefly describes conventional challenges in leukaemia theranostics, followed by the properties of P&HNP that make them potential candidates for leukaemia management. In addition, an understanding of the distinctions between natural (e.g. chitosan) and synthetic polymers (e.g. polyethene glycol), as well as their capacity to integrate targeting ligands, imaging agents, and therapeutic payloads, is essential for the rational design of next-generation treatments. Indeed, hybrid nanosystems that combine polymers with metallic nanoparticles (e.g. Au, Ag, Fe, and Zn) represent an emerging frontier, as noted. Next, the current status and clinical outcomes of P&HNP-based formulations are presented, along with their limitations and solutions to advance them as advanced therapies. Finally, challenges or obstacles that limit the translation perspective of laboratory-designed nanomedicine were addressed. This review identifies promising directions, including innovative nanoparticle designs and combination therapies, that may transform leukaemia treatment paradigms and improve patient survival.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148354951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of bioactive scaffold magnesium-containing hydroxyapatite from biomass resources for supporting osteogenesis, angiogenesis, and<i>in vivo</i>bone regeneration.","authors":"I Kadek Hariscandra Dinatha, Endang Haryati, Jumiarti Andi Lolo, Mega Andini, Saifuddin Aziz, Dinda Aliffia, Hevi Wihadmadyatami, Fikhri A Tasmara, Apri I Supii, Dian Juliadmi, Yusril Yusuf","doi":"10.1088/1748-605X/ae8b66","DOIUrl":"10.1088/1748-605X/ae8b66","url":null,"abstract":"<p><p>Translating artificial bone research into clinical applications requires numerous preclinical studies to evaluate the bioactivity of scaffolds in facilitating cell adhesion, proliferation, osteoblastic differentiation, and angiogenic differentiation. Additionally, developing materials with dual bioactivity to stimulate bone regeneration and vascularization remains a significant challenge. Previous research has successfully extracted hydroxyapatite (HA) from biomass sand lobster shells (HA-SLS;<i>Panulirus homarus</i>), which contain Mg²⁺ions that have the potential to accelerate osteoblast differentiation for bone regeneration compared with commercial HA (HA-C). The novelty of this research is the significant preclinical evaluation results of the novel HA-SLS bioactive ceramic scaffold in facilitating the adhesion and proliferation of pre-osteoblast MC3T3E1 cells, promoting human umbilical vein endothelial cells for angiogenesis, and accelerating femoral bone defect regeneration in white rats (<i>Rattus norvegicus</i>). The results showed that the HA-SLS scaffold could form an apatite layer in simulated body fluid, stimulate the attachment and proliferation of MC3T3E1 cells, and increase the levels of proangiogenesis biomarkers. Histology analysis of the<i>in vivo</i>bone tissue defect expressed in mallory and hematoxylin-eosin (HE) staining showed that the percentage of collagen fibers and the number of osteoblast cells forming bone matrix after being implantation for 6 weeks in the HA-SLS group were 29.6% and 20.83% higher than those of HA-C, respectively. These results were confirmed by x-ray radiographic images, which also showed a faster reduction in defect depth in the HA-SLS group compared than in the HA-C group. These findings indicate the promising dual bioactivity of the developed HA-SLS bioceramics. However, several limitations of this study need to be addressed in future studies, including mechanical properties, long-term safety, and large-animal studies prior to translation to clinical application.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anisotropic tissue biofabrication: conventional and advanced fabrication strategies for functional scaffolds.","authors":"Masoud Shirzad, Omid Kordi, Dasong Kim, Jungeun Choi, Priya Ranganathan, Jangbaek Han, Hae Gyun Lim, Seung Yun Nam","doi":"10.1088/1748-605X/ae8946","DOIUrl":"10.1088/1748-605X/ae8946","url":null,"abstract":"<p><p>Natural tissues possess structurally organized architectures that enable specialized mechanical and functional behaviors. Anisotropy, defined as the directional dependence of material properties, is a key characteristic observed across a wide range of biological tissues, including bone, tendon, cartilage, and vascular systems. This review provides a comprehensive overview of anisotropy in native tissues by systematically analyzing its structural origins, including collagen fiber orientation, hierarchical organization, and gradient distributions. The degree and functional significance of anisotropy across different tissues are comparatively discussed to establish design benchmarks for scaffold fabrication. Conventional fabrication methods, including freeze-casting, mechanical anchoring, and electrospinning, are evaluated for their ability to induce anisotropy, although they remain constrained by restricted architectural control and scalability. Furthermore, advanced techniques such as additive manufacturing offer enhanced precision and tunability for engineering anisotropic scaffolds. Finally, emerging hybrid strategies that combine multiple fabrication principles provide promising solutions to overcome these limitations and achieve complex, tissue-mimetic architectures. Ultimately, this work presents a robust framework for designing next-generation anisotropic scaffolds that bridge the gap between natural tissues and engineered constructs.</p>","PeriodicalId":72389,"journal":{"name":"Biomedical materials (Bristol, England)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148426835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}