Analysis & sensing最新文献_第4页

Bimetallic Nanoparticles as Electrochemical Labels in Immunosensors for the Detection of Biomarkers of Clinical Interest 双金属纳米粒子作为免疫传感器中的电化学标签，用于检测临床相关的生物标记物

IF 3.4

Analysis & sensing Pub Date : 2024-05-17 DOI: 10.1002/anse.202400017

David Valero-Calvo, Francisco Javier García-Alonso, Alfredo de la Escosura-Muñiz

引用次数: 0

Small-Molecule Fluorescent Probes for Plant Hormones and their Receptors 植物激素及其受体的小分子荧光探针

IF 3.4

Analysis & sensing Pub Date : 2024-05-14 DOI: 10.1002/anse.202400020

Zibo Lin, Junhao Xiong, Zihao Zhou, Prof. Qiong Chen, Prof. Jun Yin

{"title":"Small-Molecule Fluorescent Probes for Plant Hormones and their Receptors","authors":"Zibo Lin, Junhao Xiong, Zihao Zhou, Prof. Qiong Chen, Prof. Jun Yin","doi":"10.1002/anse.202400020","DOIUrl":"10.1002/anse.202400020","url":null,"abstract":"Plant hormones and their receptors play a crucial role in regulating plant growth and adapting to the stress environment. The exploration of interaction between plant hormones and their receptors is significant to comprehend the molecular mechanisms of plant growth and development, the response mechanisms of adaptation to environmental changes, and to optimize the traits and stress-resistance of crops. Since the biosynthesis, transport, and metabolism of hormones in plants are closely relevant to spatio-temporal changes, and their content and distribution are highly dynamic, there is an urgent need for a qualitative and quantitative tool to accurately, real-time, and in situ monitor the dynamic changes of hormones in plants without injury. Fluorescent probes have been widely used in the sensing and imaging of plant hormones and their receptors, due to their high spatio-temporal resolution, high selectivity, non-invasive, high sensitivity, and tailored molecular structures. Here, this paper provides a systematical overview of the research progress in the sensing and imaging of plant hormones and their receptors using fluorescent probes. In addition, the potential prospects and remaining challenges are also discussed to design fluorescent probes with better performance and promote the development of this field.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140977972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Stable 2D Conductive Lanthanide Organic Frameworks (Lu-HHTP) for High-Performance Humidity Sensors 为高性能湿度传感器合成稳定的二维导电镧系元素有机框架 (Lu-HHTP)

IF 3.4

Analysis & sensing Pub Date : 2024-05-09 DOI: 10.1002/anse.202400024

Dr. Shan Jiang, Xichao Mo, Xuefei Zhao, Xiaohui Yan, Zhaorui Zhang, Jiahao Liu, Huashuai Hu, Nan Wang, Prof. Minghui Yang

{"title":"Synthesis of Stable 2D Conductive Lanthanide Organic Frameworks (Lu-HHTP) for High-Performance Humidity Sensors","authors":"Dr. Shan Jiang, Xichao Mo, Xuefei Zhao, Xiaohui Yan, Zhaorui Zhang, Jiahao Liu, Huashuai Hu, Nan Wang, Prof. Minghui Yang","doi":"10.1002/anse.202400024","DOIUrl":"10.1002/anse.202400024","url":null,"abstract":"Two-dimensional conductive metal-organic frameworks (MOFs) featuring structural diversity and high porosity represent promising platforms for chemiresistive humidity sensing. The precise control of the structure of lanthanide-based MOFs and an exploration of its impact on charge transport and sensing applications have consistently been focal points for researchers. In this study, we present the synthesis and characterization of Lu-HHTP (HHTP=2,3,6,7,10,11-hexahydroxytriphenylene) as highly crystalline and conductive porous materials. The polymeric framework of Lu-HHTP encompasses 1D hexagonal channels and exhibits interlayer π–π stacking, resulting in a material with a high surface area and uniform rod-like microstructure. Benefiting from its elevated electrical conductivity, the Lu-HHTP-based humidity sensor exhibited commendable sensing properties within the relative humidity range of 33 % to 95 % at room temperature (25 °C), achieving a response value as high as 19 at 95 % relative humidity. Furthermore, the sensor displayed superior repeatability, characterized by rapid response and recovery speeds in the presence of moisture. These findings indicate that Lu-HHTP holds substantial promise as a material for humidity sensors.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140995890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances 基于表达嗅觉受体细胞的气味生物传感器：最新进展

IF 3.4

Analysis & sensing Pub Date : 2024-04-26 DOI: 10.1002/anse.202400006

Hongchao Deng, Takamichi Nakamoto

{"title":"Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances","authors":"Hongchao Deng, Takamichi Nakamoto","doi":"10.1002/anse.202400006","DOIUrl":"https://doi.org/10.1002/anse.202400006","url":null,"abstract":"Inspired by the powerful biological olfaction, scientists extracted numerous materials such as olfactory sensory neuron, olfactory receptor (OR) protein, and odorant binding protein from animal olfactory systems, then combined them with transducers to form multiple odor biosensors. These biosensors, despite well inheriting the sensing ability of creatures, have several drawbacks, such as complex preparation process, unstable sensing material characteristics, and high cost. Unlike the biological materials listed above, cell expressing heterologous OR maintains a stable sensing performance after passaging for multiple generations, also its experimental operation is simple, and cost is low. Therefore, odor biosensors based on cell expressing OR have been well developed in recent years. In this review, we first listed several odor biosensors based on cell expressing OR, mainly focusing on fluorescent and electrophysiological measurement methods. Furthermore, we illustrated the techniques to improve the biosensor performance, e. g., wider detection range, longer lifetime, more OR types, and higher quantification efficiency. In addition, we explained the possible prospects such as big sensor array and predicting odor response.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142233231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible Dual-Channel Molecularly Imprinted Electrochemical Sensor for Simultaneously Monitoring Sweat Cortisol and Lactate Levels 用于同时监测汗液皮质醇和乳酸盐水平的柔性双通道分子印迹电化学传感器

IF 3.4

Analysis & sensing Pub Date : 2024-04-23 DOI: 10.1002/anse.202400003

Siying Pei, Dr. Wenhui Ji, Ya Yang, Tianwei Liu, Shuo Yang, Prof. Dr. Jiayi Wu, Jiangxuan Dai, Dr. Xiyan Hou, Qiong Wu, Prof. Dr. Lin Li

{"title":"Flexible Dual-Channel Molecularly Imprinted Electrochemical Sensor for Simultaneously Monitoring Sweat Cortisol and Lactate Levels","authors":"Siying Pei, Dr. Wenhui Ji, Ya Yang, Tianwei Liu, Shuo Yang, Prof. Dr. Jiayi Wu, Jiangxuan Dai, Dr. Xiyan Hou, Qiong Wu, Prof. Dr. Lin Li","doi":"10.1002/anse.202400003","DOIUrl":"10.1002/anse.202400003","url":null,"abstract":"While exercise offers significant potential to enhance overall well-being, unscientific exercise practices often cause exercise fatigue, posing a threat to human health. Flexible sweat sensors have garnered considerable attention owing to their ability to continuously, non-invasively, and dynamically monitor human health during exercise at the molecular level. Therefore, in this study, we constructed a flexible molecularly imprinted polymer (MIP) sensor for the real-time monitoring of cortisol and lactate levels in sweat using cortisol or lactate as template molecules and pyrrole (Py) as functional monomer. Prussian blue (PB) was embedded into the MIP as a built-in redox probe, eliminating the need for an additional probe and facilitating the simultaneous quantification of cortisol and lactate concentrations. Moreover, the MIP-doped platinum nanoparticles (PtNPs) ehanced the electron transfer capability, further improving the sensitivity of the sensors. The fabricated flexibile cortisol and lactate MIP sensors demonstrated low limits of detection (LOD; 1.07 nM and 1.09 mM, respectively), high sensitivity (0.09 μA lg[nM]−1 and 1.28 μA lg[nM]−1), and exceptional stability and selectivity. The flexible MIP sensors could continuously and dynamically monitor changes in sweat cortisol and lactate concentrations, thus contributing to the advancement of next-generation flexible sweat electrochemical sensors and providing a crucial tool for monitoring exercise fatigue.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 5","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140667962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upconversion Luminescence Based Direct Hybridization Assay to Detect Subfemtomolar miR-20 a DNA Analogue in Plasma 基于上转换发光的直接杂交测定法检测血浆中的亚母摩尔 miR-20a DNA 类似物

IF 3.4

Analysis & sensing Pub Date : 2024-04-22 DOI: 10.1002/anse.202400005

Saara Kuusinen, Satu Lahtinen, Tero Soukka

{"title":"Upconversion Luminescence Based Direct Hybridization Assay to Detect Subfemtomolar miR-20 a DNA Analogue in Plasma","authors":"Saara Kuusinen, Satu Lahtinen, Tero Soukka","doi":"10.1002/anse.202400005","DOIUrl":"10.1002/anse.202400005","url":null,"abstract":"MicroRNAs (miRNAs) are promising biomarkers especially for early-stage cancer diagnostics, but the implementation of miRNA-based diagnostic tests is still hindered by the limitations of current analytical methods. The small size, low concentrations in biofluids and high sequence homology of miRNAs are challenges for assay development. Currently, most of the sensitive detection methods rely on enzymatic amplification steps, which complicate the analysis and can lead to biases in quantitation. Therefore, there is an increasing need to develop enzyme-free detection methods that are sensitive, specific and user-friendly. In this study, a simple direct hybridization assay for the DNA analogue of miR-20a was developed. The assay is based on upconverting nanoparticle labels, which enable ultrasensitive detection, and hairpin structured probes, which provide additional hybridization stability due to base stacking. The limit of detection was 0.73 fM with plasma recoveries between 76 % and 111 %, demonstrating that the assay could be used for direct detection of miRNAs from complex sample matrices without isolation of RNA. Due to the simplicity and the excellent sensitivity for an amplification-free method, the assay has a great potential for miRNA-based clinical applications.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140678057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Detection of Multiple Biomarkers by Peptide Nucleic Acids-Based Triplex Molecular Beacon in a Nanopore 通过纳米孔中基于多肽核酸的三重分子信标同时检测多种生物标记物

IF 3.4

Analysis & sensing Pub Date : 2024-04-17 DOI: 10.1002/anse.202400002

Dr. Bingyuan Guo, Dr. Yun Zhang, Qianyuan Ren, Dr. Ke Zhou, Dr. Lei Liu, Prof. Hai-Chen Wu

{"title":"Simultaneous Detection of Multiple Biomarkers by Peptide Nucleic Acids-Based Triplex Molecular Beacon in a Nanopore","authors":"Dr. Bingyuan Guo, Dr. Yun Zhang, Qianyuan Ren, Dr. Ke Zhou, Dr. Lei Liu, Prof. Hai-Chen Wu","doi":"10.1002/anse.202400002","DOIUrl":"10.1002/anse.202400002","url":null,"abstract":"In this study, we propose an enhanced nanopore sensing strategy that utilizes a peptide nucleic acid (PNA)-based triplex molecular beacon sensor to achieve the simultaneous detection of multiple biomarkers with a high degree of sensitivity. The sensor is a triplex switch composed of a triplex-forming DNA strand and an oligo-arginine-tagged PNA strand, serving as the target recognition moiety and signal output element, respectively. Upon target binding to the recognition element of the sensor, the PNA signal output strand is released and a hybrid complex of the target-DNA recognition strand is formed simultaneously. Due to the positive charges carried by the PNA-Arg strands, they could be driven through the nanopore under positive electric field, effectively eliminating interferences from co-existing target-DNA complexes. This approach enables label-free, one-step detection of targets without requiring complex treatments and procedures. Leveraging the modular properties of DNA recognition strand, this method can be applied universally, and here, we successfully demonstrate its application using three SARS-CoV-2 related biomarkers.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140694392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRI Probes for In Vivo Aldehyde Sensing 用于体内醛感应的核磁共振探针

IF 3.4

Analysis & sensing Pub Date : 2024-04-10 DOI: 10.1002/anse.202300087

Alexia Kirby, Mojmír Suchý, Adam J. Shuhendler

{"title":"MRI Probes for In Vivo Aldehyde Sensing","authors":"Alexia Kirby, Mojmír Suchý, Adam J. Shuhendler","doi":"10.1002/anse.202300087","DOIUrl":"10.1002/anse.202300087","url":null,"abstract":"Endogenous aldehydes are produced via tightly regulated metabolic processes and are rapidly cleared by aldehyde dehydrogenases. However, dysregulation of these processes leads to accumulation of toxic aldehydes in affected tissues, resulting in electrophilic stress forming pathogenic DNA- and protein-adducts. The highly reactive aldehydes contribute to numerous pathologies including traumatic brain injury, cancer, cardiovascular diseases, and fibrosis. Due to their transient nature and electrophilicity, the development of molecular imaging probes with the ability to trap and detect aldehydes in vivo remains a challenge. Herein, two classes of aldehyde-mapping MRI probes are discussed: (1) gadolinium and manganese-containing macrocyclic MRI agents targeting extracellular aldehydes produced during active tissue fibrosis, and (2) metal-free hydrazoCEST-MRI agents for total intracellular aldehyde detection. This comprehensive review outlines the development, mechanisms, and potential applications of diverse MRI probes targeting aldehydes, aiming to advance non-invasive diagnostic tools, disease staging, and therapeutic interventions in multiple pathologies.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140719868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal Ion-Mediated Optical Biosensors with Signal Amplification: Recent Advances 具有信号放大功能的金属离子介导光学生物传感器：最新进展

IF 3.4

Analysis & sensing Pub Date : 2024-04-07 DOI: 10.1002/anse.202400014

Lin Jiang, Chao Wang, Lijun Xu, Renjun Pei

引用次数: 0

More than just Alkene Construction – Re-Using Wittig Reactions/Reagents in Biomacromolecular Labeling, Imaging, Sequencing and Modification 不仅仅是烯烃构建--在生物分子标记、成像、测序和修饰中重复使用维蒂希反应/试剂

IF 3.4

Analysis & sensing Pub Date : 2024-03-12 DOI: 10.1002/anse.202300098

Yin-Ming He, Liang Cheng

{"title":"More than just Alkene Construction – Re-Using Wittig Reactions/Reagents in Biomacromolecular Labeling, Imaging, Sequencing and Modification","authors":"Yin-Ming He, Liang Cheng","doi":"10.1002/anse.202300098","DOIUrl":"10.1002/anse.202300098","url":null,"abstract":"Classical organic chemical reactions are essential for modern synthetic chemistry and offer valuable insights for chemical biology research. The pioneering bioorthogonal chemistry, based on the Staudinger reaction, is a prime example. However, the biocompatibility of classic “name” reactions like the Wittig reaction is still not fully explored. This versatile reaction efficiently converts carbonyl groups into olefins using phosphorus ylides, making it valuable in synthetic chemistry. Despite being in the early stages of development, the Wittig reaction and its reagents have various applications in peptide, protein, DNA, and RNA research. However, they have limitations such as low activity and efficiency, requiring organic solvents. Future directions may include developing Wittig reagents with improved biostability, simplifying the method, and creating multi-labeling methods. Improving light-activated Wittig reactions and interdisciplinary integration can further advance bioorthogonal chemistry. As technology advances, the Wittig reaction is poised to make greater contributions to molecular biology, cell biology, and biochemical modification research.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140250097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0