Analysis & sensing最新文献_第3页

Oxidoreductase-Mimicking Nanozyme-Based Biosensors for Bacteria Detection in Complex Scenarios 复杂环境下细菌检测的模拟氧化还原酶纳米酶生物传感器

IF 2.9

Analysis & sensing Pub Date : 2026-03-03 DOI: 10.1002/anse.202500230

Caiyue Wang, Jinsong Fan, Pingping Wan, Yuping Bao, Qingyun Liu, Kun Li

{"title":"Oxidoreductase-Mimicking Nanozyme-Based Biosensors for Bacteria Detection in Complex Scenarios","authors":"Caiyue Wang, Jinsong Fan, Pingping Wan, Yuping Bao, Qingyun Liu, Kun Li","doi":"10.1002/anse.202500230","DOIUrl":"https://doi.org/10.1002/anse.202500230","url":null,"abstract":"Bacterial infectious diseases pose a serious challenge to global public health, making the development of rapid, sensitive, and low-cost bacterial detection technologies critically important. Nanozymes, as a class of nanomaterials exhibiting enzyme-like activities, demonstrate unique advantages for this purpose due to their outstanding stability, tunable catalytic properties, and multifunctionality. Particularly, oxidoreductase-mimicking nanozymes exhibit great potential in biosensing. This review systematically summarizes the recent advances of their applications in bacterial detection. We discuss the design principles and synthesis methodologies of metal-based, oxide-/sulfide-based, carbon-based, and composite nanozymes, followed by their various oxidation–reduction reaction catalytic mechanisms and utilization strategies in designing bacterial assays. Their applications span clinical diagnostics, food safety monitoring, environmental surveillance, and the detection of drug-resistant bacteria and detection in special scenarios. More importantly, we focus on the strategic approaches to enhance nanozyme-based detection, including sensitivity improvement, specificity enhancement, detection mode innovation, and function expansion. Finally, the current challenges and future research directions in this field are proposed. This review provides a comprehensive reference for further research and practical application of nanozymes in bacterial detection, aiming to promote the translational application of nanozyme technology in the diagnosis and prevention of infectious diseases.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Homogeneous Digital Immunoassay for a Potential Noninvasive Urinary Biomarker of p62 for Neuronal Intranuclear Inclusion Disease 神经元核内包涵病潜在的无创尿p62生物标志物的同质数字免疫分析

IF 2.9

Analysis & sensing Pub Date : 2026-03-03 DOI: 10.1002/anse.202500222

Ye Rong, Xiaowen Li, Jin Tian, Lei Bao, Dandan Zhao, Hao Chen, Xiaojun Liu, Hongwei Gai

{"title":"A Homogeneous Digital Immunoassay for a Potential Noninvasive Urinary Biomarker of p62 for Neuronal Intranuclear Inclusion Disease","authors":"Ye Rong, Xiaowen Li, Jin Tian, Lei Bao, Dandan Zhao, Hao Chen, Xiaojun Liu, Hongwei Gai","doi":"10.1002/anse.202500222","DOIUrl":"https://doi.org/10.1002/anse.202500222","url":null,"abstract":"Neuronal intranuclear inclusion disease (NIID) is a rare, progressive neurodegenerative disorder currently diagnosed through invasive biopsies or genetic testing with ethnic limitations. Existing biomarkers from cerebrospinal fluid or plasma are not fully noninvasive, and no urine-based biomarker has been identified. There is an urgent need for a noninvasive, definitive diagnostic tool to distinguish NIID from other neurodegenerative diseases and healthy individuals. We developed a homogeneous digital immunoassay using two-color quantum dot (QD)-labeled antibodies to quantify p62 in urinary cell nuclei, achieving a detection limit of 47 fM. The assay demonstrated high selectivity and a wide dynamic range across three orders of magnitude. Analysis of urine samples from 10 NIID patients, 4 healthy controls, and 13 patients with other neurodegenerative disorders (myasthenia gravis, cerebral infarction, and Parkinson's disease) revealed that p62 levels were significantly elevated only in NIID patients. Spike recovery tests confirmed measurement accuracy (91%–106%), and p62 levels showed no correlation with disease stage in this limited cohort. This study introduces the first noninvasive urinary biomarker for NIID using a novel homogeneous digital immunoassay. The significantly elevated p62 levels specifically in NIID patients highlight its potential as a reliable, noninvasive diagnostic tool, enabling distinction from other neurodegenerative conditions and facilitating earlierand more accessible diagnosis.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric Determination of Mercury (II) in Milk and Tap Water by g-C3N4/Fe3o4 Functionalized with Gold Nanoparticles 纳米金功能化g-C3N4/Fe3o4比色法测定牛奶和自来水中的汞(II

IF 2.9

Analysis & sensing Pub Date : 2026-02-25 Epub Date: 2025-07-21 DOI: 10.1002/anse.202500093

Felicity Babuseng, Nicolette R. Hendricks-Leukes, Hailu Sheferaw Ayele, Melisew Tadele Alula

{"title":"Colorimetric Determination of Mercury (II) in Milk and Tap Water by g-C3N4/Fe3o4 Functionalized with Gold Nanoparticles","authors":"Felicity Babuseng, Nicolette R. Hendricks-Leukes, Hailu Sheferaw Ayele, Melisew Tadele Alula","doi":"10.1002/anse.202500093","DOIUrl":"10.1002/anse.202500093","url":null,"abstract":"Low stability due to aggregation of colloidal gold nanoparticles (AuNPs), utilization of environmentally unfriendly reducing agents, and high energy consumption in preparation of AuNPs are the main issues that should be addressed in AuNPs synthesis methods. Herein, for the synthesis of AuNPs, ferrous sulfate is used as a reducing agent to reduce Au3+ to Au0. Graphitic carbon nitride-iron oxide composite (g-C3N4/Fe3O4) is used as a solid support to load the AuNPs. The synthesized AuNPs-loaded g-C3N4/Fe3O4 shows peroxidase-like activity using 3,3′, 5,5′-tetramethylbenzidine (TMB) as a chromogenic agent. Addition of Hg2+ on the enzyme-mimic reaction system further enhances the peroxidase-like activity. The activity depends on the concentration of Hg2+ and a colorimetric method for determination of Hg2+ is developed. The absorbance intensity shows that the activity increases with Hg2+ ion linearly in a concentration range of 0.5–90 µM. The fitted linear regression line is used to compute the limit of detection and is found to be 0.029 µM. Milk and tap water are used to evaluate the practicability of the method. Recovery rates of 90–104% and 80–100% are obtained for analysis of milk and tap water, respectively. The performance of the nanozymes is maintained after extended time storage showing the stability of AuNPs-g-C3N4/Fe3O4. It is anticipated that this approach of synthesis method can be applied for preparation of other noble metals nanostructures.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric Determination of Mercury (II) in Milk and Tap Water by g-C3N4/Fe3o4 Functionalized with Gold Nanoparticles 纳米金功能化g-C3N4/Fe3o4比色法测定牛奶和自来水中的汞(II

IF 2.9

Analysis & sensing Pub Date : 2026-02-25 Epub Date: 2025-07-21 DOI: 10.1002/anse.202500093

Felicity Babuseng, Nicolette R. Hendricks-Leukes, Hailu Sheferaw Ayele, Melisew Tadele Alula

{"title":"Colorimetric Determination of Mercury (II) in Milk and Tap Water by g-C3N4/Fe3o4 Functionalized with Gold Nanoparticles","authors":"Felicity Babuseng, Nicolette R. Hendricks-Leukes, Hailu Sheferaw Ayele, Melisew Tadele Alula","doi":"10.1002/anse.202500093","DOIUrl":"https://doi.org/10.1002/anse.202500093","url":null,"abstract":"Low stability due to aggregation of colloidal gold nanoparticles (AuNPs), utilization of environmentally unfriendly reducing agents, and high energy consumption in preparation of AuNPs are the main issues that should be addressed in AuNPs synthesis methods. Herein, for the synthesis of AuNPs, ferrous sulfate is used as a reducing agent to reduce Au3+ to Au0. Graphitic carbon nitride-iron oxide composite (g-C3N4/Fe3O4) is used as a solid support to load the AuNPs. The synthesized AuNPs-loaded g-C3N4/Fe3O4 shows peroxidase-like activity using 3,3′, 5,5′-tetramethylbenzidine (TMB) as a chromogenic agent. Addition of Hg2+ on the enzyme-mimic reaction system further enhances the peroxidase-like activity. The activity depends on the concentration of Hg2+ and a colorimetric method for determination of Hg2+ is developed. The absorbance intensity shows that the activity increases with Hg2+ ion linearly in a concentration range of 0.5–90 µM. The fitted linear regression line is used to compute the limit of detection and is found to be 0.029 µM. Milk and tap water are used to evaluate the practicability of the method. Recovery rates of 90–104% and 80–100% are obtained for analysis of milk and tap water, respectively. The performance of the nanozymes is maintained after extended time storage showing the stability of AuNPs-g-C3N4/Fe3O4. It is anticipated that this approach of synthesis method can be applied for preparation of other noble metals nanostructures.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-Enhanced Raman Spectroscopy for Acrylamide Detection Using Gold Nanoparticles on Titanium Oxide Nanowires: A Green Analytical Method 氧化钛纳米线上金纳米粒子表面增强拉曼光谱检测丙烯酰胺：一种绿色分析方法

IF 2.9

Analysis & sensing Pub Date : 2026-02-17 DOI: 10.1002/anse.202500198

Maria Tarapoulouzi, Marios Stavrou, Marios Constantinou, Aliki Souzou, Lygia Eleftheriou, Chrysafis Andreou

{"title":"Surface-Enhanced Raman Spectroscopy for Acrylamide Detection Using Gold Nanoparticles on Titanium Oxide Nanowires: A Green Analytical Method","authors":"Maria Tarapoulouzi, Marios Stavrou, Marios Constantinou, Aliki Souzou, Lygia Eleftheriou, Chrysafis Andreou","doi":"10.1002/anse.202500198","DOIUrl":"10.1002/anse.202500198","url":null,"abstract":"Acrylamide (Aam) is a potentially carcinogenic compound formed in heat-processed, starch-rich foods, posing significant food safety concerns even at low dietary exposures. Food safety regulations mandate regular testing for Aam at the point of food production; however, current analytical methods for Aam are time-consuming and costly. In this study, we developed a green, label-free analytical method based on surface-enhanced Raman spectroscopy (SERS) using gold nanoparticles (Au NP) grown on titanium oxide (TiO2) nanowires (NWs) as the active substrate. The TiO2 NWs provide a scaffold with high surface-area for uniform Au NP placement, producing a substrate that enhances the Raman signal of Aam through plasmonic effects. A portable, low-cost Raman spectrometer was used for data acquisition. The method demonstrated a broad detection range from 10 μM to 10 mM, relevant to the regulatory benchmarks for common foods. The analytical performance of the sensor was validated using chemometrics. This study establishes a sustainable and effective SERS-based approach for future monitoring of Aam in food, supporting improved food safety and public health protection.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202500198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147288267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Acoustic Ejection Mass Spectrometry to Accelerate Drug Discovery 声射质谱法在加速药物发现中的应用

IF 2.9

Analysis & sensing Pub Date : 2026-02-17 DOI: 10.1002/anse.202500218

Juncai Meng, Erwin Abucayon, Michael Wleklinski, Shasha Li, Chang Liu

引用次数: 0

Front Cover: Exploring Coumarin Derivatives for High-Performance Metal Ion Detection (Anal. Sens. 2/2025) 封面：探索香豆素衍生物在高性能金属离子检测中的应用。参议员2/2025)

IF 2.9

Analysis & sensing Pub Date : 2026-02-15 DOI: 10.1002/anse.70067

Yelyzaveta V. Antsybora, Viktoriia S. Moskvina, Volodymir P. Khilya

引用次数: 0

Green Synthesis of Tannic Acid-Modified Gold Nanozymes for Rapid and Selective Colorimetric Detection of Fe3+, H2O2, and I− 单宁酸修饰金纳米酶快速选择性比色检测Fe3+， H2O2和I−的绿色合成

IF 2.9

Analysis & sensing Pub Date : 2026-02-15 DOI: 10.1002/anse.202500205

Xin-Yi Lin, Chen-Yu Chueh, Tsunghsueh Wu, Yang-Wei Lin

{"title":"Green Synthesis of Tannic Acid-Modified Gold Nanozymes for Rapid and Selective Colorimetric Detection of Fe3+, H2O2, and I−","authors":"Xin-Yi Lin, Chen-Yu Chueh, Tsunghsueh Wu, Yang-Wei Lin","doi":"10.1002/anse.202500205","DOIUrl":"10.1002/anse.202500205","url":null,"abstract":"A cost-effective and rapid colorimetric sensing platform was developed to overcome the high cost and operational complexity of traditional analytical methods for detecting ferric ions (Fe3+), hydrogen peroxide (H2O2), and iodide ions (I−). Tannic acid-modified gold nanoparticles (TA-AuNPs) were synthesized via a one-pot method, where tannic acid (TA) acted as both reducing and stabilizing agent. The resulting TA-AuNPs exhibited excellent stability under acidic, alkaline, and high-salinity conditions and demonstrated peroxidase-like catalytic activity. In the presence of Fe3+, TA-AuNPs catalyzed the H2O2-mediated oxidation of 3,3′, 5,5′-tetramethylbenzidine (TMB) to its blue oxidized form (ox-TMB), showing a linear response from 20 to 100 µM with a limit of detection (LOD) of 1.34 µM. For H2O2, a linear range of 30–150 µM and an LOD of 10.1 µM were achieved. TA-AuNPs also catalyzed the oxidation of I− to triiodide (I3−), producing a color change from pink to yellow with a peak at 350 nm. The I− sensor showed an LOD of 2.38 μM and >100.8% recovery in real water samples. This multifunctional TA-AuNP nanozyme platform provides a simple, sensitive, and selective approach for Fe3+, H2O2, and I− detection, with promising applications in environmental monitoring, public health, and food safety.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146680374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Sensitivity of Mn Kβ X-Ray Fluorescence Line Splitting for Manganese Valence State Quantification in Manganese Ores Mn Kβ x射线荧光谱线分裂测定锰矿石中锰价态的化学灵敏度

IF 2.9

Analysis & sensing Pub Date : 2026-02-10 DOI: 10.1002/anse.202500206

Ashok Kumar Maurya

{"title":"Chemical Sensitivity of Mn Kβ X-Ray Fluorescence Line Splitting for Manganese Valence State Quantification in Manganese Ores","authors":"Ashok Kumar Maurya","doi":"10.1002/anse.202500206","DOIUrl":"10.1002/anse.202500206","url":null,"abstract":"Mn Kβ X-ray fluorescence line splitting arising from 3p5–3dn exchange interactions, is highly sensitive to the number of unpaired 3d electrons, serving as a powerful probe for manganese oxidation states. Because these spectral components are separated by only 11–12 eV, they are typically unresolved by standard laboratory spectrometers, necessitating synchrotron-based high-resolution X-ray emission spectrometers. These techniques provide the required electronic-structure sensitivity. However, their dependence on synchrotron facilities limits accessibility. This article presents an optimized laboratory-based Wavelenth Dispersive X-ray Fluorescence protocol that achieves high-resolution spectra without hardware modification. By utilizing higher-order diffraction, the method successfully resolves subtle variations in the Mn Kβ main line multiplet. The extent of splitting is quantified using the integrated absolute difference metric referenced to spin-zero KMnO4. This approach yields a strong linear correlation (R2 = 0.9947) with unpaired 3d electrons, enabling the direct determination of the average oxidation state (AOS). By combining spectroscopic AOS with Mn (IV) values in a linear mixing model, the protocol resolves Mn(II) and Mn(III) fractions with < 4% relative error. This accessible methodology provides a practical, accurate alternative to synchrotron facilities for quantitative manganese speciation in complex geological materials.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146162557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Portable Stochastic Sensor With Poly-Arginine and Bimetallic Nanoparticles on Reduced Graphene Oxide for Simultaneous AFP and CA 72-4 Detection in Gastric Cancer 基于还原氧化石墨烯的聚精氨酸和双金属纳米颗粒绿色便携式随机传感器用于同时检测胃癌中AFP和CA 72-4

IF 2.9

Analysis & sensing Pub Date : 2026-02-10 DOI: 10.1002/anse.202500175

Ramona Georgescu-State, Ionela Raluca Comnea-Stancu, Raluca-Ioana Stefan-van Staden, Ruxandra-Maria Ilie-Mihai

{"title":"Green Portable Stochastic Sensor With Poly-Arginine and Bimetallic Nanoparticles on Reduced Graphene Oxide for Simultaneous AFP and CA 72-4 Detection in Gastric Cancer","authors":"Ramona Georgescu-State, Ionela Raluca Comnea-Stancu, Raluca-Ioana Stefan-van Staden, Ruxandra-Maria Ilie-Mihai","doi":"10.1002/anse.202500175","DOIUrl":"10.1002/anse.202500175","url":null,"abstract":"Alpha-fetoprotein (AFP) and carbohydrate antigen 72-4 (CA 72-4) are among the most clinically relevant biomarkers in gastric cancer, providing critical pre- and postoperative information to clinicians. To address the limitations of conventional assays, we developed a portable, green stochastic sensor based on a screen-printed carbon electrode (SPCE) modified with poly-arginine (poly-Arg) and Pd–Ni@rGO bimetallic nanoparticles for the simultaneous determination of AFP and CA 72-4 in diverse biological samples. Pd–Ni@rGO nanoparticles were synthesized via a facile chemical reduction technique. The sensor exhibited wide linear dynamic ranges and ultralow limits of quantification (LOQ), ranging from 3 × 10−16 to 3 × 10−7 mg mL−1 (LOQ: 3 × 10−16 mg mL−1) for AFP and 4 × 10−11 to 4 × 10−8 U mL−1 (LOQ: 4 × 10−11 U mL−1) for CA 72-4. Comparative analysis using the Student's t-test confirmed no statistically significant difference between the results obtained with the proposed sensor and reference methods at a 99% confidence level. The greenness and applicability of the method were further validated using GAPI, AGREE, and BAGI assessment tools, confirming both low environmental impact and strong practical utility. This work represents a significant advancement in electrochemical sensing, providing an innovative and sustainable strategy for clinically relevant AFP and CA 72-4 detection.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146154846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0