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Cover Feature: Identification of DNA Aptamers for Benzodiazepine Detection in Synthetic Urine (Anal. Sens. 1/2023) 封面特征：用于检测合成尿液中苯二氮卓类药物的DNA适配子的鉴定（Anal.Sens.1/2023）
Analysis & sensing Pub Date : 2022-11-27 DOI: 10.1002/anse.202200107
John S. Samuelian, Dr. Dana A. Baum
{"title":"Cover Feature: Identification of DNA Aptamers for Benzodiazepine Detection in Synthetic Urine (Anal. Sens. 1/2023)","authors":"John S. Samuelian,&nbsp;Dr. Dana A. Baum","doi":"10.1002/anse.202200107","DOIUrl":"https://doi.org/10.1002/anse.202200107","url":null,"abstract":"<p><b>The cover feature image illustrates</b> a newly reported biosensor intended to reliably monitor patient use and compliance of benzodiazepines. Akin to the epidemic rise in use and abuse of this drug class, the artist has placed the biosensor literally under the magnifying glass hoping to draw attention to an improved and unique DNA aptamer-based detection method for testing patient urine. The cover art was designed by Marc Polaske and was inspired by noir detective graphic novels. More information can be found in the Research Article by John S. Samuelian and Dana A. Baum.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202200107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50123205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorescent RNA Tags for In Situ RNA Imaging in Living Cells 用于活细胞原位RNA成像的荧光RNA标签
Analysis & sensing Pub Date : 2022-11-24 DOI: 10.1002/anse.202200090
Peng Yin, Dr. Shi Kuang, Prof. Zhou Nie
{"title":"Fluorescent RNA Tags for In Situ RNA Imaging in Living Cells","authors":"Peng Yin,&nbsp;Dr. Shi Kuang,&nbsp;Prof. Zhou Nie","doi":"10.1002/anse.202200090","DOIUrl":"https://doi.org/10.1002/anse.202200090","url":null,"abstract":"<p>RNA imaging paves the way to investigate RNA function and regulation from the aspect of RNA subcellular localization and expression level. In the past decades, various highly precise and genetic coding fluorescent RNA, which can bind and light up the fluorophore by their specific sequence/structure, has been developed for RNA imaging, including Broccoli, Mango, SRB-2, RhoBAST, and Pepper et al. In this Review, we summarize the RNA imaging approaches and the progress made in the development of fluorescent RNA. Next, we emphasize the application of fluorescent RNA for in situ RNA imaging in living cells. Of particular note, besides the artificially selected fluorescent RNAs, a new kind of fluorophore that can bind and light up intrinsic RNA structure is described together for the first time. Finally, a summary of current strategies for designing fluorescent RNA and future perspectives are also presented. We hope this expanding topic will inspire the study of the architecture and functions of these fascinating molecular machinery in biological systems.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50143230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover Feature: Multiplexed Label-Free Biomarker Detection by Targeted Disassembly of Variable-Length DNA Payload Chains (Anal. Sens. 4/2023) 封面特征：通过靶向拆卸可变长度DNA有效载荷链进行多重无标记生物标志物检测（Anal.Sens.4/2023）
Analysis & sensing Pub Date : 2022-11-22 DOI: 10.1002/anse.202200102
Matthew Aquilina, Katherine E. Dunn
{"title":"Cover Feature: Multiplexed Label-Free Biomarker Detection by Targeted Disassembly of Variable-Length DNA Payload Chains (Anal. Sens. 4/2023)","authors":"Matthew Aquilina,&nbsp;Katherine E. Dunn","doi":"10.1002/anse.202200102","DOIUrl":"https://doi.org/10.1002/anse.202200102","url":null,"abstract":"<p><b>The cover feature image illustrates</b> the detection principle behind a novel multiplexed biomarker detection platform. The presence of specific biomarkers releases distinctly-sized DNA fragments, resulting in a characteristic band pattern in electrophoresis experiments. In combination with capillary electrophoresis, this could enable automated detection of multiple biomarkers in parallel, at a fraction of the cost and complexity of conventional diagnostic systems. Cover design by Matthew Aquilina. More information can be found in the Research Article by Matthew Aquilina and Katherine E. Dunn.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202200102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50141262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover Feature: Isothermal Titration Calorimetry for Characterizing the Zinc(II)-Binding Properties of Photocaged Complexes with Micromolar Affinity (Anal. Sens. 2/2023) 封面特征：表征具有微极性亲和性的光老化配合物的锌（II）结合性质的等温滴定量热法（Anal.Sens.2/2023）
Analysis & sensing Pub Date : 2022-11-22 DOI: 10.1002/anse.202200101
Erin E. Hickey, Dr. Austin Shigemoto, Prof. Dr. Arne Gericke, Prof. Dr. Shawn C. Burdette
{"title":"Cover Feature: Isothermal Titration Calorimetry for Characterizing the Zinc(II)-Binding Properties of Photocaged Complexes with Micromolar Affinity (Anal. Sens. 2/2023)","authors":"Erin E. Hickey,&nbsp;Dr. Austin Shigemoto,&nbsp;Prof. Dr. Arne Gericke,&nbsp;Prof. Dr. Shawn C. Burdette","doi":"10.1002/anse.202200101","DOIUrl":"https://doi.org/10.1002/anse.202200101","url":null,"abstract":"<p><b>The cover feature image shows</b> alternate methods to characterize photocages by illustrating the photolysis of the bis-complex of the PyrDeCage, a low affinity photocage for divalent zinc. The uncaging involves a photodecarboxylation of a <i>meta</i>-nitrophenylacetic acid caging group to make weakly binding zinc ligands. The zinc release can be useful for investigating the impact of zinc ions in biology or other areas. The characterization of the zinc-binding properties required exploring new methodologies including isothermal titration calorimetry that uses thermodynamic data to provide binding constants. Cover designed by Chelsea A. Barr. More information can be found in the Research Article by Shawn C. Burdette and co-workers.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202200101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50140932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Highly Accurate pH Detection Method for Sweat Analysis using a Printed 96-Microwell Colorimetric Sensor Array 使用印刷96微孔比色传感器阵列进行汗液分析的高精度pH检测方法
Analysis & sensing Pub Date : 2022-11-19 DOI: 10.1002/anse.202200097
Dr. Yui Sasaki, Xiaojun Lyu, Prof. Tsuyoshi Minami
{"title":"A Highly Accurate pH Detection Method for Sweat Analysis using a Printed 96-Microwell Colorimetric Sensor Array","authors":"Dr. Yui Sasaki,&nbsp;Xiaojun Lyu,&nbsp;Prof. Tsuyoshi Minami","doi":"10.1002/anse.202200097","DOIUrl":"https://doi.org/10.1002/anse.202200097","url":null,"abstract":"<p>This work reports on a facile 96-microwell paper-based colorimetric sensor array device (PCSAD) for highly accurate pH detection toward sweat analysis. Although a pH test strip has been widely used to easily visualize pH conditions with naked eyes, its accuracy to guarantee below the decimal point in pH changes has still been a concern. In contrast, the PCSAD could detect slight pH changes because of abundant chemical information by the array design. The color gradients on the PCSAD upon changing pH conditions can be rapidly recorded by a flatbed scanner. The captured digital images of the PCSAD were treated with imaging analysis, followed by extraction of color intensities for inset data. By employing the partially overlapped detectable pH ranges of pH indicators, seamless inset data was obtained. Therefore, the PCSAD successfully discriminated against slight changes in pH conditions in human sweat with high accuracy by pattern recognition methods.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50138281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiplexed Label-Free Biomarker Detection by Targeted Disassembly of Variable-Length DNA Payload Chains 靶向拆卸可变长度DNA有效载荷链检测多重无标记生物标志物
Analysis & sensing Pub Date : 2022-11-17 DOI: 10.1002/anse.202200082
Matthew Aquilina, Katherine E. Dunn
{"title":"Multiplexed Label-Free Biomarker Detection by Targeted Disassembly of Variable-Length DNA Payload Chains","authors":"Matthew Aquilina,&nbsp;Katherine E. Dunn","doi":"10.1002/anse.202200082","DOIUrl":"https://doi.org/10.1002/anse.202200082","url":null,"abstract":"<p>Simultaneously studying different biomarker types (DNA, RNA, proteins, etc.) could improve understanding and diagnosis of many complex diseases. However, biomarker detection involves several complex or expensive methodologies, requiring specialized laboratories and personnel. A multiplexed assay would greatly facilitate the use of biomarker data. Here, we present a multiplexed biomarker detection technique using variable-length DNA payload chains, which are systematically disassembled in the presence of specific biomarkers. The resulting distinctly-sized fragments yield characteristic gel electrophoresis band patterns. This has enabled us to detect with high sensitivity and specificity DNA sequences including BRCA1 (limit of detection, LOD, ∼3 nM), RNA (miR-141, LOD ∼19 nM) and the steroids aldosterone and cortisol (LOD ∼200–250 nM). We show that our assay is multiplexable, and suffers limited sensitivity loss in fetal bovine serum and can be applied using capillary electrophoresis, which may be more amenable to automation and integration in healthcare settings.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202200082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50151846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does Charge Influence the Response in Oxyanion Binding Europium(III) Complexes? 电荷会影响氧阴离子结合铕（III）配合物的反应吗？
Analysis & sensing Pub Date : 2022-11-07 DOI: 10.1002/anse.202200093
Simon Hery, Dr. Laura L. A. Grenier, Dr. Thomas J. Sørensen
{"title":"Does Charge Influence the Response in Oxyanion Binding Europium(III) Complexes?","authors":"Simon Hery,&nbsp;Dr. Laura L. A. Grenier,&nbsp;Dr. Thomas J. Sørensen","doi":"10.1002/anse.202200093","DOIUrl":"https://doi.org/10.1002/anse.202200093","url":null,"abstract":"<p>Through the synthesis of a positively charged europium complex, with a structure derived from the DO2A (1, 4, 7, 10-tetraazacyclododecane-1, 7-diacetic acid), we were able to explore the influence of charge on the interaction between coordinatively unsaturated lanthanide complexes and charged oxyanions. The data suggest that the positive charge of the lanthanide host does not allow better selectivity between the differently and negatively charged oxyanion guests. Although, binding constants seem greatly improved with charge for the <i>α</i>-hydroxycarboxylates investigated here.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50137970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A Fluorescent Sensor Array for the Discrimination of Nucleotide Phosphates 用于识别核苷酸磷酸盐的荧光传感器阵列
Analysis & sensing Pub Date : 2022-11-02 DOI: 10.1002/anse.202200089
Dr. Genevieve E. Sergeant, Dr. Vincent E. Zwicker, Prof. Katrina A. Jolliffe
{"title":"A Fluorescent Sensor Array for the Discrimination of Nucleotide Phosphates","authors":"Dr. Genevieve E. Sergeant,&nbsp;Dr. Vincent E. Zwicker,&nbsp;Prof. Katrina A. Jolliffe","doi":"10.1002/anse.202200089","DOIUrl":"https://doi.org/10.1002/anse.202200089","url":null,"abstract":"<p>Chemosensors that can discriminate between nucleotide phosphates are difficult to prepare. Here we describe the use of a sensing array comprising metal complexes of bis(dipicolylamino)-functionalised linear peptide based fluorescent sensors for the discrimination of nucleotide phosphates. We demonstrate that through the rational incorporation of additional sensor elements the discriminatory power of the array can be improved. An array incorporating two peptides in combination with four metal ions was able to successfully discriminate all triphosphates and diphosphates tested. When monophosphates were included in the analysis, 91 % of the analytes (blank, AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP and UTP) could be correctly classified, with all classification errors related to monophosphates.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202200089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50118491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Affinity-Based Wearable Electrochemical Biosensors: Natural versus Biomimetic Receptors 基于亲和性的可穿戴电化学生物传感器：天然受体与仿生受体
Analysis & sensing Pub Date : 2022-11-02 DOI: 10.1002/anse.202200087
Prof. Susana Campuzano, Prof. María Pedrero, Dr. Rebeca M. Torrente-Rodríguez, Prof. José M. Pingarrón
{"title":"Affinity-Based Wearable Electrochemical Biosensors: Natural versus Biomimetic Receptors","authors":"Prof. Susana Campuzano,&nbsp;Prof. María Pedrero,&nbsp;Dr. Rebeca M. Torrente-Rodríguez,&nbsp;Prof. José M. Pingarrón","doi":"10.1002/anse.202200087","DOIUrl":"https://doi.org/10.1002/anse.202200087","url":null,"abstract":"<p>This review delves into the titanic research efforts carried out during the last years on affinity-based wearable electrochemical biosensors, using both natural (antibodies) and biomimetic (aptamers, peptides and molecular imprinted polymers) receptors. The rationale and application of selected representative strategies is critically discussed, ending with realistic and futuristic visions of the technical barriers, challenges and prospects in the development and adoption of these biodevices in daily routines to ensure well-being against known, unknown and unexpected threats.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202200087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50122282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Dissolution Dynamic Nuclear Polarization of the 77Se Nucleus 77Se核的溶解动力学核极化
Analysis & sensing Pub Date : 2022-10-31 DOI: 10.1002/anse.202200086
Dr. Eul Hyun Suh, Dr. James Ratnakar, Dr. Jaspal Singh, Prof. Zoltan Kovacs
{"title":"Dissolution Dynamic Nuclear Polarization of the 77Se Nucleus","authors":"Dr. Eul Hyun Suh,&nbsp;Dr. James Ratnakar,&nbsp;Dr. Jaspal Singh,&nbsp;Prof. Zoltan Kovacs","doi":"10.1002/anse.202200086","DOIUrl":"https://doi.org/10.1002/anse.202200086","url":null,"abstract":"<p><sup>77</sup>Se is a spin <math>\u0000 \u0000 <semantics>\u0000 \u0000 <mrow>\u0000 <mn>1</mn>\u0000 <mo>/</mo>\u0000 <mn>2</mn>\u0000 </mrow>\u0000 \u0000 <annotation>\u0000 ${{ 1/2 }}$\u0000</annotation>\u0000 </semantics>\u0000 </math>\u0000, low sensitivity nucleus with a natural abundance of 7.6 %. Although <sup>77</sup>Se NMR is very useful in the characterization of selenium containing molecules including seleno-proteins, the detection of <sup>77</sup>Se is challenging in biological samples without enrichment. Therefore, the goal of this work was to establish whether the <sup>77</sup>Se signal could be enhanced in the liquid state by dissolution dynamic nuclear polarization (DNP) NMR without the need of enrichment. The dominant spin-lattice relaxation mechanism for <sup>77</sup>Se is via chemical shift anisotropy, which is highly dependent on the molecular symmetry. Here we tested three selenium compounds (sodium selenate, sodium selenite and selenocystine) with different molecular symmetries in dissolution DNP experiments and demonstrated that <sup>77</sup>Se DNP using commercially available hardware is feasible but the achieved NMR signal enhancements (1368-fold for selenate, 125-fold for selenite and no enhancement for selenocystine at 9.4 T) were strongly dependent on molecular symmetry.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50149496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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