活细胞中乙醇脱氢酶活性的荧光成像研究

IF 3.4 Q2 CHEMISTRY, ANALYTICAL
Wai Yin Yau, Dr. Samuel Kin-Man Lai, Dr. Pilar Blasco, Prof. Xuechen Li, Prof. Kwan Ming Ng, Dr. Chun Nam Lok, Dr. Ho Yu Au-Yeung
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引用次数: 0

摘要

本文报道了一种基于活性检测乙醇脱氢酶活性的荧光探针的开发。笼状香豆素报告基因在酶的选择性氧化作用下被释放,释放强度增强60倍。该探针具有较低的细胞毒性,已被用于观察HepG2、A549和HEK293T细胞中的乙醇脱氢酶活性,证明了其作为一种方便易用的生物分析工具在揭示该酶在酒精代谢中的作用方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Activity-based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells

Activity-based Fluorescent Imaging of Alcohol Dehydrogenase Activity in Living Cells

Development of a fluorescent probe for activity-based sensing of activity of alcohol dehydrogenase, a key enzyme in ethanol biooxidation, is reported. A caged coumarin reporter is released upon the selective oxidation by the enzyme with a strong, >60-fold emission enhancement. The probe has a low cytotoxicity and has been applied in visualising alcohol dehydrogenase activity in HepG2, A549 and HEK293T cells, demonstrating its potential as a convenient, easy-to-use bioanalytical tools in unveiling the roles of the enzyme in alcohol metabolism.

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CiteScore
2.60
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