Analysis & sensing最新文献_第2页

An Aptameric Sensor for Water Pollutant Dimethylsilanediol: From Capture-SELEX to Bead-Based Fluorescence Assay 水污染物二甲基硅二醇的适体传感器：从捕获selex到基于珠的荧光测定

IF 3.3

Analysis & sensing Pub Date : 2026-09-09 DOI: 10.1002/anse.70123

Niloofar Zolfigol, Baikun Li, Paul Nahass, Yu Lei

{"title":"An Aptameric Sensor for Water Pollutant Dimethylsilanediol: From Capture-SELEX to Bead-Based Fluorescence Assay","authors":"Niloofar Zolfigol, Baikun Li, Paul Nahass, Yu Lei","doi":"10.1002/anse.70123","DOIUrl":"https://doi.org/10.1002/anse.70123","url":null,"abstract":"An aptamer-based sensing strategy was developed for the detection of dimethylsilanediol (DMSD), an emerging contaminant identified in International Space Station (ISS) water systems. Target-specific aptamers were isolated using a modified systematic evolution of ligands by exponential enrichment (SELEX) approach, capture-SELEX, enabling the selection of high-affinity binders for DMSD molecules. The two selected aptamers were characterized using a magnetic bead-based fluorescence assay, yielding apparent dissociation constants (KD,app) of 18 and 162 nM, respectively. The same platform was subsequently applied to detect DMSD across a wide concentration range. This fluorescence-based approach enables quantitative detection through a simple workflow without complex instrumentation, making it well-suited for resource-limited environments such as the ISS. The assay achieves high sensitivity and allows direct calibration via target-induced aptamer displacement without additional signal amplification. Overall, this work establishes a practical, resource-efficient aptamer-based sensing strategy for monitoring DMSD contamination in water systems, with particular relevance to closed-loop life support environments for long-duration space missions. The developed aptamer-based sensing strategy provides a versatile platform that could be adapted and extended to detect other environmental contaminants.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Multiplex Immunoassay for Bacteria Detection via Surface-Enhanced Raman Spectroscopy by Using a Combination of Nanoaggregate-Embedded Beads and Magnetic Nanoparticles 表面增强拉曼光谱细菌检测的多重免疫分析——纳米聚合体嵌入微珠和磁性纳米颗粒的结合

IF 3.3

Analysis & sensing Pub Date : 2026-09-05 DOI: 10.1002/anse.70126

Kuan-Ying Li, Chih-Hsien Wang, Chia-Chun Chu, Shih-Ying Yen, Huai-Chang Wu, Shi-Ting Wang, Lai-Kwan Chau, Chiou-Ying Yang

{"title":"Rapid Multiplex Immunoassay for Bacteria Detection via Surface-Enhanced Raman Spectroscopy by Using a Combination of Nanoaggregate-Embedded Beads and Magnetic Nanoparticles","authors":"Kuan-Ying Li, Chih-Hsien Wang, Chia-Chun Chu, Shih-Ying Yen, Huai-Chang Wu, Shi-Ting Wang, Lai-Kwan Chau, Chiou-Ying Yang","doi":"10.1002/anse.70126","DOIUrl":"https://doi.org/10.1002/anse.70126","url":null,"abstract":"Rapid detection of multiple bacterial pathogens has become a recent research focus. Traditional detection methods are often complex and time-consuming, highlighting the need for simpler and faster techniques capable of simultaneously identifying multiple bacterial pathogens. This study selects Salmonella choleraesuis and Neisseria lactamica as a model for simultaneous multiplex detection of dual pathogens. We employed an ultrahigh sensitivity surface-enhanced Raman scattering nanotag, termed nanoaggregate-embedded bead (NAEB), and combined with bioconjugated magnetic nanoparticles (MNP) to magnetically capture and detect the target bacteria. In this study, methylene blue (MB) and cresyl violet (CV) were selected as the Raman reporters to make the NAEBs. Then, the NAEBs were separately functionalized with an antibody specific to Salmonella choleraesuis or Neisseria lactamica. Additionally, Concanavalin A (ConA) was used to functionalize MNP to form the MNP@ConA bioconjugate, which can simultaneously capture both Salmonella choleraesuis and Neisseria lactamica in solution, achieving rapid magnetic separation and preconcentration within a total analysis time of 40 min. Results show that qualitative and quantitative detection of pathogenic bacteria is possible over the concentration range from 10 to 105 CFU/mL. When detecting each pathogen individually, the detection limits for Salmonella choleraesuis and Neisseria lactamica are 5.1 and 8.2 CFU/mL, respectively.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria-Targeted Fluorescent Probes and the Biomedical Applications: A Minireview 线粒体靶向荧光探针及其生物医学应用综述

IF 3.3

Analysis & sensing Pub Date : 2026-09-03 DOI: 10.1002/anse.70118

Hui Cai, Xin-Yue Zhang, Tian-Yi Yin, Zhang Ye, Hui-Ru Lu, Qing Zhu, Yi Hu

{"title":"Mitochondria-Targeted Fluorescent Probes and the Biomedical Applications: A Minireview","authors":"Hui Cai, Xin-Yue Zhang, Tian-Yi Yin, Zhang Ye, Hui-Ru Lu, Qing Zhu, Yi Hu","doi":"10.1002/anse.70118","DOIUrl":"https://doi.org/10.1002/anse.70118","url":null,"abstract":"Mitochondria are central regulators of cellular metabolism and cell death, and thus represent critical targets for understanding the mechanisms of major diseases, including cancer and neurodegenerative disorders. Real-time monitoring of mitochondrial microenvironmental changes, functional parameters, and bioactive molecules is crucial for elucidating pathological processes. The fluorescent probes have emerged as powerful tools for this purpose. In recent years, mitochondria-targeted fluorescent probes have advanced rapidly, enabling the visualization of representative mitochondrial parameters and molecular targets, including temperature, viscosity, reactive oxygen/nitrogen species, reactive sulfur species, and metabolic indicators. Rather than attempting to provide an exhaustive classification of all reported mitochondrial probes, this review focuses on representative examples that illustrate how probe design and mitochondrial imaging contribute to disease mechanism studies, drug screening and evaluation, disease diagnosis, lesion visualization, and cancer theranostics. We further discuss current challenges and future directions, including targeting specificity, quantitative reliability, multiparameter imaging, and clinical translation. This review is expected to inspire the development of mitochondrial probe-based strategies for disease diagnosis and treatment.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148942201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Memristor-Based Sensing–Memory–Computing Integrated Sensors: Mechanisms, Materials, Architectures, and Applications 基于忆阻器的传感-存储-计算集成传感器：机制、材料、架构和应用

IF 3.3

Analysis & sensing Pub Date : 2026-08-31 DOI: 10.1002/anse.70120

Zilu Zhao, Xiaolan Luo, Jun Wang, Xiangsheng Chen, Hong Yuan, Yuanjie Su

{"title":"Memristor-Based Sensing–Memory–Computing Integrated Sensors: Mechanisms, Materials, Architectures, and Applications","authors":"Zilu Zhao, Xiaolan Luo, Jun Wang, Xiangsheng Chen, Hong Yuan, Yuanjie Su","doi":"10.1002/anse.70120","DOIUrl":"https://doi.org/10.1002/anse.70120","url":null,"abstract":"Conventional sensing systems based on physically separated sensing, memory, and computing units suffer from substantial data-transfer overhead, latency, and energy consumption, limiting their deployment in edge intelligence and the Internet of Things. Memristive in-sensor computing has emerged as a promising hardware paradigm by integrating stimulus perception, state retention, and information processing within a single device or array. This review discusses memristor-based sensing-memory-computing integrated sensors from four perspectives: response modality, material system, fabrication and integration strategy, and device architecture. Working mechanisms and sensing behaviors are classified into digital memristors for event-driven functions and binary or low-bit synapses, and analog memristors for gradual weight modulation, feature extraction, and high-precision neuromorphic computing. Representative material platforms, including oxides, two-dimensional materials, organic materials, halide perovskites, electrochemical metallization systems, ferroelectric systems, and phase-change and correlated materials, are compared in terms of operating mechanisms, stimulus-coupling pathways, advantages, and limitations. The effects of thin-film deposition, post-treatment, patterning, and array integration on device uniformity, manufacturability, and scalability are further discussed. Finally, recent progress in visual, tactile, and biomimetic perception is reviewed, and key challenges and future directions are outlined for next-generation systems for edge intelligence.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk-Based Platforms for Biochemical Sensors 生物化学传感器的丝基平台

IF 3.3

Analysis & sensing Pub Date : 2026-08-30 DOI: 10.1002/anse.70122

Xinyi Chen, Jinzhang Guo, Lin Zhu, Xiufang Li, Yanfei Feng, Renchuan You

{"title":"Silk-Based Platforms for Biochemical Sensors","authors":"Xinyi Chen, Jinzhang Guo, Lin Zhu, Xiufang Li, Yanfei Feng, Renchuan You","doi":"10.1002/anse.70122","DOIUrl":"https://doi.org/10.1002/anse.70122","url":null,"abstract":"Continuous monitoring of ions, metabolites, gases, and disease biomarkers is essential for precision health, environmental surveillance, and food safety. However, biochemical sensors often face challenges in wet and complex media, where biofouling, leaching, and unstable mass transport can lead to signal drift and compromise quantitative reliability. Silk offers a promising protein-based material platform for addressing these issues, leveraging its hierarchical structure, tunable physicochemical properties, and diverse processing routes. In this review, we summarize the development of silk-based biochemical sensors from three key construction strategies: structural regulation and derivatization of silk protein materials, functional composition, biointerface engineering, and device coupling. We classify the roles of silk materials as a support and immobilization matrix, functional sensing medium, or sampling and system-integration layer. These roles are discussed alongside electrochemical and optical readout mechanisms, with emphasis on stability, sensitivity, and practical integration. Finally, we outline the current essential requirements for practical applications, focusing on mechanistic understanding, interfacial stability, quantitative analysis under real-world conditions, scalable manufacturing, and system-level integration. We also highlight future opportunities and challenges for silk-based biochemical sensors.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148943457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Probing the Structure and Thermoresponsive Behavior of Multicompartment Micelles by Combined Solution- and Solid-State NMR Spectroscopy 用固溶核磁共振光谱法探测多室胶束的结构和热响应行为

IF 3.3

Analysis & sensing Pub Date : 2026-08-21 DOI: 10.1002/anse.70113

Justyna Żmuda, Ann-Christin Pöppler

{"title":"Probing the Structure and Thermoresponsive Behavior of Multicompartment Micelles by Combined Solution- and Solid-State NMR Spectroscopy","authors":"Justyna Żmuda, Ann-Christin Pöppler","doi":"10.1002/anse.70113","DOIUrl":"https://doi.org/10.1002/anse.70113","url":null,"abstract":"Multicompartment micelles (MCMs) that are formed from thermoresponsive triblock copolymers offer an interesting option for drug delivery systems. However, structural rearrangements are challenging to characterize. Here, we combine solution- and solid-state NMR spectroscopy with complementary techniques (DLS, DSC, PXRD) to investigate two potentially MCM-forming copolymers: methoxy poly(ethylene glycol)-block-poly(butyl acrylate)-block-poly‌‌(N-‍isopropylacrylamide) (PBuAN250) and methoxy poly(ethylene glycol)-block-poly(benzyl acrylate)-block-poly(N-isopropylacrylamide) (PBzAN300). P-fraction values were extracted from variable-temperature 1H NMR to quantify the lower critical solution temperature (LCST) transition of poly(N-isopropylacrylamide) (PNIPAM), revealing how core rigidity affects the transition. This was complemented by T2 relaxation time data to further probe dynamics of chains upon rearrangement. Nuclear Overhauser effect spectroscopy (NOESY) was used to reveal spatial proximities between different polymer blocks. As PNIPAM becomes undetectable in solution above the LCST, solid-state NMR was used to fill that gap. 1H T1 relaxation time measurements were employed on thermally treated freeze-dried micelles to show convergence of PNIPAM and butyl acrylate (BuA) block relaxation times consistent with PNIPAM relocating into the hydrophobic core. 1H-13C heteronuclear correlation (HETCOR) spectra further revealed subtle local changes in chain arrangements, overall demonstrating how comprehensive NMR characterization captures structure-dynamics relationships and paints a consistent molecular-level picture difficult to access with other methods.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.70113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148785078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Planar to Patterned: Structured Boron-Doped Diamond Electrodes for Electrochemical Sensing 从平面到图案：电化学传感用结构掺硼金刚石电极

IF 3.3

Analysis & sensing Pub Date : 2026-08-19 DOI: 10.1002/anse.70112

Ghazaleh Kholafazadehastamal, Karolina Schwarzová-Pecková, Simona Baluchová

{"title":"From Planar to Patterned: Structured Boron-Doped Diamond Electrodes for Electrochemical Sensing","authors":"Ghazaleh Kholafazadehastamal, Karolina Schwarzová-Pecková, Simona Baluchová","doi":"10.1002/anse.70112","DOIUrl":"https://doi.org/10.1002/anse.70112","url":null,"abstract":"Structured boron-doped diamond (BDD) electrodes have evolved beyond planar interfaces into a promising platform for electrochemical sensing. By introducing deliberately engineered micro- and nanostructures, the electrode–electrolyte interface can be tailored to enhance analytical performance while preserving the excellent properties of BDD, including wide potential window, chemical robustness, and resistance to fouling. Although the first sensing studies on structured BDD appeared in the late 2000s and the field has expanded markedly since then, no review has yet focused specifically on structured BDD for sensing and electroanalysis. This mini-review fills that gap by summarizing the reported top-down and bottom-up fabrication strategies and the resulting architectures, including nanowires, nanograss, porous films, and template-derived three-dimensional structures. Their applications are discussed across major analyte classes, from neurotransmitters and other biologically relevant small molecules to biomacromolecules and toxic heavy metal ions. Particular emphasis is placed on emerging structure–property–performance relationships, showing that the benefits of structuring cannot be explained by surface area increase alone, but arise from the interplay of morphology, accessibility, mass transport, and local surface chemistry. Current challenges and future directions are also outlined, especially with respect to reproducibility, scalability, and device integration.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and Highly Sensitive H2 Sensor Based on Ag-Modified ZnO Nanoflowers 基于ag修饰ZnO纳米花的快速高灵敏度H2传感器

IF 3.3

Analysis & sensing Pub Date : 2026-08-19 DOI: 10.1002/anse.70119

Zexu Zhao, Lin Tao, Mingyang Gu, Xin Quan, Xingyu Zhu, Baigang An, Lixiang Li

{"title":"Fast and Highly Sensitive H2 Sensor Based on Ag-Modified ZnO Nanoflowers","authors":"Zexu Zhao, Lin Tao, Mingyang Gu, Xin Quan, Xingyu Zhu, Baigang An, Lixiang Li","doi":"10.1002/anse.70119","DOIUrl":"https://doi.org/10.1002/anse.70119","url":null,"abstract":"Rapid and sensitive detection of trace-level H2 is essential for hydrogen energy safety and industrial monitoring, yet remains challenging. Herein, Ag-modified ZnO nanoflowers are developed for fast and ultrasensitive H2 sensing. Structural and surface analyses reveal that Ag forms Schottky contacts with ZnO and significantly promotes the generation of active oxygen species. The optimized Ag–ZnO sensor exhibits a high response of 59.4 toward 100 ppm H2 at 275 °C, which is approximately 13.5 times higher than that of pristine ZnO (4.4), together with an ultrafast response time of 4 s and a theoretical detection limit of 5.1 ppb. Moreover, the sensor demonstrates excellent repeatability, long-term stability, and high selectivity against common interfering gases. The enhanced sensing performance originates from the synergistic effects of Schottky junction-induced resistance modulation and oxygen species-mediated surface reaction enhancement, where Ag increases the baseline resistance and facilitates H2 oxidation. This work provides an effective strategy for constructing high-performance ppb-level H2 sensors with potential applications in hydrogen safety monitoring and energy-related systems.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148784636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Chemiluminescent Sensor for Sensitive Determination of Total Antioxidant Capacity in Commercial Products 一种灵敏测定商品中总抗氧化能力的化学发光传感器

IF 3.3

Analysis & sensing Pub Date : 2026-08-19 DOI: 10.1002/anse.70111

Xin Wu, Fengrong Lv, Hengxin Shen, Jinxue Xiang, Jiahui Cai, Fan Yang, Shuangyan Huan, Zhe Li, Ying Zhou, Hao-Ran Liu, Guosheng Song

{"title":"A Chemiluminescent Sensor for Sensitive Determination of Total Antioxidant Capacity in Commercial Products","authors":"Xin Wu, Fengrong Lv, Hengxin Shen, Jinxue Xiang, Jiahui Cai, Fan Yang, Shuangyan Huan, Zhe Li, Ying Zhou, Hao-Ran Liu, Guosheng Song","doi":"10.1002/anse.70111","DOIUrl":"https://doi.org/10.1002/anse.70111","url":null,"abstract":"Total antioxidant capacity (TAC) is essential for evaluating the quality and efficacy of commercial products like beverages and skincare items, yet current detection methods often lack simplicity, sensitivity, and reliability. Here, we developed a chemiluminescent sensor based on Chlorin e6 nanoparticles (Ce6 NPs) and Mn7+ for rapid and straightforward TAC detection. The sensor, fabricated by self-assembling Ce6 with amphiphilic surfactant DSPE-mPEG followed by Mn7+ incorporation, exhibited 34-fold enhanced chemiluminescence compared to Ce6 NPs alone. The system showed high specificity for Mn7+, with a limit of detection (LOD) of 0.93 μM. Mechanistic studies indicated that Mn7+ generates reactive oxygen species (ROS), triggering Ce6 chemiluminescence. Taking advantage of the redox reaction between antioxidants and Mn7+, the sensor enabled sensitive quantification of glutathione and ascorbic acid, with LOD of 0.24 and 0.026 mM, respectively. The practical utility of the sensor was demonstrated by evaluating the TAC of five beverages and five skincare products, revealing significant differences among samples. Among beverages, beverage #5 showed the highest TAC; among skincare products, serum #5 exhibited superior antioxidant capacity. Overall, this Ce6 NPs–Mn7+ chemiluminescence sensor offers key advantages—rapidity, simplicity, high sensitivity, and low cost—making it a promising tool for TAC assessment in commercial applications.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Competitive-Equilibrium Fluorescent Aptasensor for Label-Free Kanamycin Detection With Integrated Recognition and Signal Output 具有集成识别和信号输出的无标签卡那霉素检测竞争平衡荧光配体传感器

IF 3.3

Analysis & sensing Pub Date : 2026-08-18 DOI: 10.1002/anse.70116

Keren Chen, Lingyun Wang, Qingfang Zheng, Longjiao Zhu, Liangjuan Zhao, Wenjie Zheng, Wentao Xu

{"title":"A Competitive-Equilibrium Fluorescent Aptasensor for Label-Free Kanamycin Detection With Integrated Recognition and Signal Output","authors":"Keren Chen, Lingyun Wang, Qingfang Zheng, Longjiao Zhu, Liangjuan Zhao, Wenjie Zheng, Wentao Xu","doi":"10.1002/anse.70116","DOIUrl":"https://doi.org/10.1002/anse.70116","url":null,"abstract":"Kanamycin residues pose potential risks to food safety and public health, highlighting the need for rapid, low-cost, and sensitive detection methods. However, developing label-free aptasensors that integrate target recognition and signal output remains challenging. Herein, a label-free fluorescent aptasensor was developed using a SMART-derived fluorescent aptamer and a competitive-equilibrium biosensing mechanism. The fluorescent aptamer MC-DA-LG was employed as an integrated signal-output and kanamycin-responsive aptamer module. MC-DA-LG generated a fluorescence signal upon binding to the fluorescent molecule, whereas the addition of kanamycin resulted in a concentration-dependent fluorescence decrease, suggesting a shift in the aptamer–dye binding equilibrium. Circular dichroism spectroscopy indicated conformational changes in MC-DA-LG upon the addition of kanamycin or malachite green, while molecular docking suggested partially overlapping binding regions for the two small molecules, collectively supporting the proposed mechanism. Under optimized conditions, the biosensor showed a linear response from 0.1 to 10 μM, with an ultralow detection limit of 400 pM. Moreover, the biosensor exhibited favorable selectivity over other antibiotics and satisfactory recoveries of 94.1%–97.3% in milk and fetal bovine serum samples. This simple, low-cost, rapid, and ultrasensitive assay enables direct fluorescence detection without labeling, immobilization, or complex procedures, demonstrating the potential of SMART-tailored fluorescent aptamers for integrated label-free biosensing of kanamycin.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"6 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148783562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0