Analysis & sensing最新文献_第2页

Flexible Dual-Channel Molecularly Imprinted Electrochemical Sensor for Simultaneously Monitoring Sweat Cortisol and Lactate Levels 用于同时监测汗液皮质醇和乳酸盐水平的柔性双通道分子印迹电化学传感器

IF 3.4

Analysis & sensing Pub Date : 2024-04-23 DOI: 10.1002/anse.202400003

Siying Pei, Dr. Wenhui Ji, Ya Yang, Tianwei Liu, Shuo Yang, Prof. Dr. Jiayi Wu, Jiangxuan Dai, Dr. Xiyan Hou, Qiong Wu, Prof. Dr. Lin Li

{"title":"Flexible Dual-Channel Molecularly Imprinted Electrochemical Sensor for Simultaneously Monitoring Sweat Cortisol and Lactate Levels","authors":"Siying Pei, Dr. Wenhui Ji, Ya Yang, Tianwei Liu, Shuo Yang, Prof. Dr. Jiayi Wu, Jiangxuan Dai, Dr. Xiyan Hou, Qiong Wu, Prof. Dr. Lin Li","doi":"10.1002/anse.202400003","DOIUrl":"10.1002/anse.202400003","url":null,"abstract":"While exercise offers significant potential to enhance overall well-being, unscientific exercise practices often cause exercise fatigue, posing a threat to human health. Flexible sweat sensors have garnered considerable attention owing to their ability to continuously, non-invasively, and dynamically monitor human health during exercise at the molecular level. Therefore, in this study, we constructed a flexible molecularly imprinted polymer (MIP) sensor for the real-time monitoring of cortisol and lactate levels in sweat using cortisol or lactate as template molecules and pyrrole (Py) as functional monomer. Prussian blue (PB) was embedded into the MIP as a built-in redox probe, eliminating the need for an additional probe and facilitating the simultaneous quantification of cortisol and lactate concentrations. Moreover, the MIP-doped platinum nanoparticles (PtNPs) ehanced the electron transfer capability, further improving the sensitivity of the sensors. The fabricated flexibile cortisol and lactate MIP sensors demonstrated low limits of detection (LOD; 1.07 nM and 1.09 mM, respectively), high sensitivity (0.09 μA lg[nM]−1 and 1.28 μA lg[nM]−1), and exceptional stability and selectivity. The flexible MIP sensors could continuously and dynamically monitor changes in sweat cortisol and lactate concentrations, thus contributing to the advancement of next-generation flexible sweat electrochemical sensors and providing a crucial tool for monitoring exercise fatigue.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140667962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upconversion Luminescence Based Direct Hybridization Assay to Detect Subfemtomolar miR-20 a DNA Analogue in Plasma 基于上转换发光的直接杂交测定法检测血浆中的亚母摩尔 miR-20a DNA 类似物

IF 3.4

Analysis & sensing Pub Date : 2024-04-22 DOI: 10.1002/anse.202400005

Saara Kuusinen, Satu Lahtinen, Tero Soukka

{"title":"Upconversion Luminescence Based Direct Hybridization Assay to Detect Subfemtomolar miR-20 a DNA Analogue in Plasma","authors":"Saara Kuusinen, Satu Lahtinen, Tero Soukka","doi":"10.1002/anse.202400005","DOIUrl":"10.1002/anse.202400005","url":null,"abstract":"MicroRNAs (miRNAs) are promising biomarkers especially for early-stage cancer diagnostics, but the implementation of miRNA-based diagnostic tests is still hindered by the limitations of current analytical methods. The small size, low concentrations in biofluids and high sequence homology of miRNAs are challenges for assay development. Currently, most of the sensitive detection methods rely on enzymatic amplification steps, which complicate the analysis and can lead to biases in quantitation. Therefore, there is an increasing need to develop enzyme-free detection methods that are sensitive, specific and user-friendly. In this study, a simple direct hybridization assay for the DNA analogue of miR-20a was developed. The assay is based on upconverting nanoparticle labels, which enable ultrasensitive detection, and hairpin structured probes, which provide additional hybridization stability due to base stacking. The limit of detection was 0.73 fM with plasma recoveries between 76 % and 111 %, demonstrating that the assay could be used for direct detection of miRNAs from complex sample matrices without isolation of RNA. Due to the simplicity and the excellent sensitivity for an amplification-free method, the assay has a great potential for miRNA-based clinical applications.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140678057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Detection of Multiple Biomarkers by Peptide Nucleic Acids-Based Triplex Molecular Beacon in a Nanopore 通过纳米孔中基于多肽核酸的三重分子信标同时检测多种生物标记物

IF 3.4

Analysis & sensing Pub Date : 2024-04-17 DOI: 10.1002/anse.202400002

Dr. Bingyuan Guo, Dr. Yun Zhang, Qianyuan Ren, Dr. Ke Zhou, Dr. Lei Liu, Prof. Hai-Chen Wu

{"title":"Simultaneous Detection of Multiple Biomarkers by Peptide Nucleic Acids-Based Triplex Molecular Beacon in a Nanopore","authors":"Dr. Bingyuan Guo, Dr. Yun Zhang, Qianyuan Ren, Dr. Ke Zhou, Dr. Lei Liu, Prof. Hai-Chen Wu","doi":"10.1002/anse.202400002","DOIUrl":"10.1002/anse.202400002","url":null,"abstract":"In this study, we propose an enhanced nanopore sensing strategy that utilizes a peptide nucleic acid (PNA)-based triplex molecular beacon sensor to achieve the simultaneous detection of multiple biomarkers with a high degree of sensitivity. The sensor is a triplex switch composed of a triplex-forming DNA strand and an oligo-arginine-tagged PNA strand, serving as the target recognition moiety and signal output element, respectively. Upon target binding to the recognition element of the sensor, the PNA signal output strand is released and a hybrid complex of the target-DNA recognition strand is formed simultaneously. Due to the positive charges carried by the PNA-Arg strands, they could be driven through the nanopore under positive electric field, effectively eliminating interferences from co-existing target-DNA complexes. This approach enables label-free, one-step detection of targets without requiring complex treatments and procedures. Leveraging the modular properties of DNA recognition strand, this method can be applied universally, and here, we successfully demonstrate its application using three SARS-CoV-2 related biomarkers.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140694392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRI Probes for In Vivo Aldehyde Sensing 用于体内醛感应的核磁共振探针

IF 3.4

Analysis & sensing Pub Date : 2024-04-10 DOI: 10.1002/anse.202300087

Alexia Kirby, Mojmír Suchý, Adam J. Shuhendler

{"title":"MRI Probes for In Vivo Aldehyde Sensing","authors":"Alexia Kirby, Mojmír Suchý, Adam J. Shuhendler","doi":"10.1002/anse.202300087","DOIUrl":"10.1002/anse.202300087","url":null,"abstract":"Endogenous aldehydes are produced via tightly regulated metabolic processes and are rapidly cleared by aldehyde dehydrogenases. However, dysregulation of these processes leads to accumulation of toxic aldehydes in affected tissues, resulting in electrophilic stress forming pathogenic DNA- and protein-adducts. The highly reactive aldehydes contribute to numerous pathologies including traumatic brain injury, cancer, cardiovascular diseases, and fibrosis. Due to their transient nature and electrophilicity, the development of molecular imaging probes with the ability to trap and detect aldehydes in vivo remains a challenge. Herein, two classes of aldehyde-mapping MRI probes are discussed: (1) gadolinium and manganese-containing macrocyclic MRI agents targeting extracellular aldehydes produced during active tissue fibrosis, and (2) metal-free hydrazoCEST-MRI agents for total intracellular aldehyde detection. This comprehensive review outlines the development, mechanisms, and potential applications of diverse MRI probes targeting aldehydes, aiming to advance non-invasive diagnostic tools, disease staging, and therapeutic interventions in multiple pathologies.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140719868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal Ion-Mediated Optical Biosensors with Signal Amplification: Recent Advances 具有信号放大功能的金属离子介导光学生物传感器：最新进展

IF 3.4

Analysis & sensing Pub Date : 2024-04-07 DOI: 10.1002/anse.202400014

Lin Jiang, Chao Wang, Lijun Xu, Renjun Pei

引用次数: 0

More than just Alkene Construction – Re-Using Wittig Reactions/Reagents in Biomacromolecular Labeling, Imaging, Sequencing and Modification 不仅仅是烯烃构建--在生物分子标记、成像、测序和修饰中重复使用维蒂希反应/试剂

IF 3.4

Analysis & sensing Pub Date : 2024-03-12 DOI: 10.1002/anse.202300098

Yin-Ming He, Liang Cheng

{"title":"More than just Alkene Construction – Re-Using Wittig Reactions/Reagents in Biomacromolecular Labeling, Imaging, Sequencing and Modification","authors":"Yin-Ming He, Liang Cheng","doi":"10.1002/anse.202300098","DOIUrl":"10.1002/anse.202300098","url":null,"abstract":"Classical organic chemical reactions are essential for modern synthetic chemistry and offer valuable insights for chemical biology research. The pioneering bioorthogonal chemistry, based on the Staudinger reaction, is a prime example. However, the biocompatibility of classic “name” reactions like the Wittig reaction is still not fully explored. This versatile reaction efficiently converts carbonyl groups into olefins using phosphorus ylides, making it valuable in synthetic chemistry. Despite being in the early stages of development, the Wittig reaction and its reagents have various applications in peptide, protein, DNA, and RNA research. However, they have limitations such as low activity and efficiency, requiring organic solvents. Future directions may include developing Wittig reagents with improved biostability, simplifying the method, and creating multi-labeling methods. Improving light-activated Wittig reactions and interdisciplinary integration can further advance bioorthogonal chemistry. As technology advances, the Wittig reaction is poised to make greater contributions to molecular biology, cell biology, and biochemical modification research.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300098","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140250097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid Characterization of Phospholipids from Biological Matrix Enabled by Indium Tin Oxide (ITO) Coated Slide Assisted Enrichment MALDI Mass Spectrometry 利用氧化铟锡 (ITO) 涂层玻片辅助富集 MALDI 质谱法快速表征生物基质中的磷脂

Analysis & sensing Pub Date : 2024-02-28 DOI: 10.1002/anse.202300097

Penghsuan Huang, Dr. Hua Zhang, Yuan Liu, Prof. Lingjun Li

{"title":"Rapid Characterization of Phospholipids from Biological Matrix Enabled by Indium Tin Oxide (ITO) Coated Slide Assisted Enrichment MALDI Mass Spectrometry","authors":"Penghsuan Huang, Dr. Hua Zhang, Yuan Liu, Prof. Lingjun Li","doi":"10.1002/anse.202300097","DOIUrl":"10.1002/anse.202300097","url":null,"abstract":"Lipidomic analysis of human serum is essential to monitor the individual's health status. Herein, we develop a facile strategy for rapid characterization of phospholipids in human serum via indium tin oxide (ITO) coated glass slide solid phase extraction MALDI mass spectrometry (ITO-SPE-MALDI-MS). Phospholipid species are retained on ITO slide via solid phase extraction owing to the unique property of the ITO material; the measurement of phospholipid species from 1 μl human serum within 2 min is achievable. A comparison of ITO-SPE strategy with conventional extraction methods was further carried out using liquid chromatography-mass spectrometry (LC-MS) and ion-mobility mass spectrometry (IM-MS), resulting in a comparable enrichment performance for the phospholipid analysis. Furthermore, rapid lipidomic profiling of serum samples from human colorectal cancer patients and cell lines was demonstrated. Our results indicate that ITO-SPE-MALDI-MS provides a higher throughput strategy for the analysis of phospholipid species in complex biological mixtures, showcasing its potential for applications in the analysis of clinical biofluids.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140422110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic Resistance Detection in Pseudomonas aeruginosa: Recent Strategies, Advances, and Challenges 铜绿假单胞菌的抗生素耐药性检测：最新策略、进展与挑战

IF 3.4

Analysis & sensing Pub Date : 2024-02-20 DOI: 10.1002/anse.202300058

Luma Clarindo Lopes, Dr. Ashkan Koushanpour, Kolby Wiebe, Prof. Dr. Sabine Kuss

{"title":"Antibiotic Resistance Detection in Pseudomonas aeruginosa: Recent Strategies, Advances, and Challenges","authors":"Luma Clarindo Lopes, Dr. Ashkan Koushanpour, Kolby Wiebe, Prof. Dr. Sabine Kuss","doi":"10.1002/anse.202300058","DOIUrl":"10.1002/anse.202300058","url":null,"abstract":"Antibiotic resistance presents a worldwide public health emergency, impeding the effective management of infectious diseases. Pseudomonas aeruginosa plays a substantial role as a bacterial pathogen, particularly in infections among hospitalized individuals, and those with weakened immune systems. Timely and accurate detection of antimicrobial resistance in P. aeruginosa is crucial for initiating tailored antibiotic therapy promptly, thus improving patient outcomes. Nevertheless, this endeavor encounters challenges due to the intricate and varied nature of antibiotic-resistant strains. Extensive efforts have been invested in developing sensors and instrumentations for assessing antibiotic resistance or susceptibility (AST), aiming to enable personalized patient treatment with appropriate medications. This paper focuses on recent advancements in these methodologies, utilized for evaluating the degree of antibiotic resistance or susceptibility in pathogenic bacteria. Flow cytometry, dual molecular recognition and mass spectrometry are presented as newer phenotypic AST techniques. Genomic methods and the pyocyanin detection are listed as methodologies for the detection of resistance indicators.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140448095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Multimodal Bio-Sensing and Imaging Platform (MSIP) for Real-Time Intraoperative Margin Detection in Cancer Surgery 用于癌症手术术中实时边缘检测的多模态生物传感和成像平台 (MSIP)

Analysis & sensing Pub Date : 2024-02-16 DOI: 10.1002/anse.202300066

Ghazal Hatami-Fard, Khushi Vyas, Daniel Leff, Ranan Dasgupta, Salzitsa Anastasova

{"title":"A Multimodal Bio-Sensing and Imaging Platform (MSIP) for Real-Time Intraoperative Margin Detection in Cancer Surgery","authors":"Ghazal Hatami-Fard, Khushi Vyas, Daniel Leff, Ranan Dasgupta, Salzitsa Anastasova","doi":"10.1002/anse.202300066","DOIUrl":"10.1002/anse.202300066","url":null,"abstract":"In cancer surgery, the precise characterisation of tissue margins is of paramount importance to ensure effective resection. However, conventional methods have inherent limitations in terms of both speed and accuracy, often necessitating labour-intensive postsurgical tissue analyses. To overcome these challenges and enhance the precision of cancer surgery, we introduced an innovative Multimodal Sensing and Imaging Platform (MSIP) equipped with advanced pH sensing capabilities. The MSIP represents the synergistic fusion of a miniaturised, flexible wire-based electrochemical pH sensor with a sensitivity of 62.4 mV per pH for real-time functional imaging. Simultaneously, it incorporates a high-speed confocal endomicroscope utilizing a 2.6 mm fibre bundle to achieve subcellular resolution in morphological imaging. The pH sensor enables continuous monitoring of dynamic changes in the tumour microenvironment, offering valuable insights. Confocal endomicroscopy provides high-resolution, real-time imaging of tumour morphology with an impressive 2.2 μm resolution. This integration not only enhances the precision of cancer surgery but also fosters a comprehensive understanding of the biological and molecular characteristics of tumours. By consolidating multiple diagnostic parameters into a unified platform, MSIP has emerged as a transformative technology poised to revolutionise our approach to cancer surgery.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140454409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles 褐藻素测定法：测量脂质囊泡中的阴离子转运

Analysis & sensing Pub Date : 2024-02-13 DOI: 10.1002/anse.202300093

Matúš Chvojka, Anurag Singh, Alessio Cataldo, Dr. Aaron Torres-Huerta, Dr. Marcin Konopka, Prof. Vladimír Šindelář, Dr. Hennie Valkenier

引用次数: 0