Analysis & sensing最新文献_第2页

Signal Amplification by Reversible Exchange and its Translation to Hyperpolarized Magnetic Resonance Imaging in Biomedicine 可逆交换的信号放大及其在生物医学超极化磁共振成像中的应用

IF 3.4

Analysis & sensing Pub Date : 2024-08-05 DOI: 10.1002/anse.202400039

Dr. Andreas B. Schmidt, Prof. Dr. Eduard Y. Chekmenev, Henri de Maissin, Philipp R. Groß, Stefan Petersen, Luca Nagel, Prof. Dr. Franz Schilling, Dr. Ilai Schwartz, Prof. Dr. Thomas Reinheckel, Prof. Dr. Jan-Bernd Hövener, Dr. Stephan Knecht

{"title":"Signal Amplification by Reversible Exchange and its Translation to Hyperpolarized Magnetic Resonance Imaging in Biomedicine","authors":"Dr. Andreas B. Schmidt, Prof. Dr. Eduard Y. Chekmenev, Henri de Maissin, Philipp R. Groß, Stefan Petersen, Luca Nagel, Prof. Dr. Franz Schilling, Dr. Ilai Schwartz, Prof. Dr. Thomas Reinheckel, Prof. Dr. Jan-Bernd Hövener, Dr. Stephan Knecht","doi":"10.1002/anse.202400039","DOIUrl":"https://doi.org/10.1002/anse.202400039","url":null,"abstract":"Hyperpolarized magnetic resonance imaging (HP-MRI) has emerged as a powerful tool in molecular imaging, providing in vivo, real-time insights into metabolic pathways without ionizing radiation. Signal Amplification by Reversible Exchange (SABRE) represents a promising hyperpolarization technique, leveraging parahydrogen to enhance MRI signals. In this concept, we delineate the evolution of SABRE and landmark papers that have enabled us recently to produce biocompatible and low-cost hyperpolarized pyruvate within minutes for in vivo metabolic imaging, showcasing SABRE′s potential for preclinical and near-future clinical settings. Looking ahead, with ongoing efforts focused on optimizing polarizer technology and expanding applications beyond pyruvate, we envision SABRE as a key player in the research and application of HP-MRI due to its simplicity and throughput.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142641358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Front Cover: (Anal. Sens. 4/2024) 封面：（Anal. Sens. 4/2024）

IF 3.4

Analysis & sensing Pub Date : 2024-07-16 DOI: 10.1002/anse.202480401

引用次数: 0

Analysis of 5-Methyl Cytosine Containing Oligomers Via Reduction of Tetracationic Di-Viologen Derivatives Using Square Wave Voltammetry 利用方波伏安法分析通过还原四阳离子二缩二脲衍生物获得的含 5-甲基胞嘧啶的低聚物

IF 3.4

Analysis & sensing Pub Date : 2024-07-12 DOI: 10.1002/anse.202400011

Victoria G. Preston, Eli G. Hvastkovs

{"title":"Analysis of 5-Methyl Cytosine Containing Oligomers Via Reduction of Tetracationic Di-Viologen Derivatives Using Square Wave Voltammetry","authors":"Victoria G. Preston, Eli G. Hvastkovs","doi":"10.1002/anse.202400011","DOIUrl":"10.1002/anse.202400011","url":null,"abstract":"5-Methylcytosine (5 mC) is an epigenetic modification that plays several important roles ranging from development to disease progression. Local cytosine methylation is still detected and quantified utilizing bisulfite cytosine to uracil conversion and methylated base counting following PCR. Electrochemical approaches may offer more rapid means to provide this output. Here, small oligomeric DNA sequences from the CD8+ T-cell reporter gene featuring between 0 and 4 5 mC sites were immobilized on Au electrodes via self-assembly. 5 mC content was assayed via square wave voltametric reduction of a di-viologen molecule (C12H25V2+C6H12V2+C12H25, V2+=4,4’-bipyridyl or viologen) bound to the DNA. 5 mC presence induced subtle structural changes in the oligomers, which could be detected monitoring di-viologen reduction current changes at −0.39 V vs. SCE after exposure to higher ionic strength conditions. Solution phase CD spectroscopy was employed to validate the DNA structural changes occurring at the electrode surface and provide insight into the structural perturbations resulting from 5 mC presence. Overall, this method provides a relatively simple electrochemical 5 mC monitoring approach for small gene sequences based on helical structure.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"5 1","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141652772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomarker Multiplexing with Rational Design of Nucleic Acid Probe Complex 通过合理设计核酸探针复合物实现生物标记多重化

IF 3.4

Analysis & sensing Pub Date : 2024-07-10 DOI: 10.1002/anse.202400009

Yu Zhao, Hui Xin, Chunyan Wang

引用次数: 0

High Selectivity MEMS C2H2 Sensor for Transformer Fault Characteristic Gas Detection** 用于变压器故障特征气体检测的高选择性 MEMS C2H2 传感器**

IF 3.4

Analysis & sensing Pub Date : 2024-06-26 DOI: 10.1002/anse.202400032

Yifeng Xu, Haixia Mei, Yu Bing, Fuyun Zhang, Ning Sui, Assoc. Prof. Tingting Zhou, Xiaopeng Fan, Lijie Wang, Prof. Tong Zhang

{"title":"High Selectivity MEMS C2H2 Sensor for Transformer Fault Characteristic Gas Detection**","authors":"Yifeng Xu, Haixia Mei, Yu Bing, Fuyun Zhang, Ning Sui, Assoc. Prof. Tingting Zhou, Xiaopeng Fan, Lijie Wang, Prof. Tong Zhang","doi":"10.1002/anse.202400032","DOIUrl":"https://doi.org/10.1002/anse.202400032","url":null,"abstract":"Acetylene (C2H2), as an important characteristic gas in transformer fault diagnosis, should be accurately detected and effectively distinguished from other dissolved gases (H2, CH4, C2H6, C2H4, CO, CO2), which is crucial to determine whether the fault occurs and the fault type, but also faces challenges now. The rational design and employment of rare earth and noble metals are expected to address this issue. In this work, SnO2-3 at% Sm2O3-1 at% PdO based MEMS gas sensor was prepared to achieve high performance detection of C2H2 which has a response value of 56 to 50 ppm C2H2, response/recovery time of 2 s/136 s, lower detection limit of 1 ppm, power consumption of 15.5 mW, and weak cross sensitivity to other transformer fault characteristic gases. Lewis acids and bases theory was used to explain the reason why rare earth Sm is a benefit element to improve selectivity to C2H2. The formation of oxygen vacancies and hetero junctions was used to explain the increased sensitivity of the material. This study proved the feasibility of rare earth and noble metals as potential additives to enable advanced gas-sensitive materials for highly selective transformer fault characteristic gas C2H2 detection.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solvent Effects in Hyperpolarization of 15N Nuclei in [15N3]Metronidazole and [15N3]Nimorazole Antibiotics via SABRE-SHEATH** 通过 SABRE-SHEATH** 使[15N3]甲硝唑和[15N3]硝咪唑抗生素中的 15N 核超极化的溶剂效应

IF 3.4

Analysis & sensing Pub Date : 2024-06-20 DOI: 10.1002/anse.202400045

Anna P. Yi, Dr. Oleg G. Salnikov, Dr. Dudari B. Burueva, Dr. Nikita V. Chukanov, Prof. Eduard Y. Chekmenev, Prof. Igor V. Koptyug

{"title":"Solvent Effects in Hyperpolarization of 15N Nuclei in [15N3]Metronidazole and [15N3]Nimorazole Antibiotics via SABRE-SHEATH**","authors":"Anna P. Yi, Dr. Oleg G. Salnikov, Dr. Dudari B. Burueva, Dr. Nikita V. Chukanov, Prof. Eduard Y. Chekmenev, Prof. Igor V. Koptyug","doi":"10.1002/anse.202400045","DOIUrl":"https://doi.org/10.1002/anse.202400045","url":null,"abstract":"Metronidazole and nimorazole are antibiotics of a nitroimidazole group which also may be potentially utilized as hypoxia radiosensitizers for the treatment of cancerous tumors. Hyperpolarization of 15N nuclei in these compounds using SABRE-SHEATH (Signal Amplification By Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei) approach provides dramatic enhancement of detection sensitivity of these analytes using magnetic resonance spectroscopy and imaging. Methanol-d4 is conventionally employed as a solvent in SABRE hyperpolarization process. Herein, we investigate SABRE-SHEATH hyperpolarization of isotopically labeled [15N3]metronidazole and [15N3]nimorazole in nondeuterated methanol and ethanol solvents. Optimization of such hyperpolarization parameters as polarization transfer magnetic field, temperature, parahydrogen flow rate and pressure allowed us to obtain an average 15N polarization of up to 7.2–7.4 % for both substrates. The highest 15N polarizations were observed in methanol-d4 for [15N3]metronidazole and in ethanol for [15N3]nimorazole. At a clinically relevant magnetic field of 1.4 T the 15N nuclei of these substrates possess long characteristic hyperpolarization lifetimes (T1) of ca. 1 to ca. 7 min. This study represents a major step toward SABRE in more biocompatible solvents, such as ethanol, and also paves the way for future utilization of these hyperpolarized nitroimidazoles as molecular contrast agents for MRI visualization of tumors.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Advances in Aptamer-Based Sensors for In Vitro Detection of Small Molecules 用于体外检测小分子的基于色聚体的传感器的最新进展

IF 3.4

Analysis & sensing Pub Date : 2024-06-20 DOI: 10.1002/anse.202400027

Yueru Mu, Zhenzhen Chen, Jiayin Zhan, Prof. Jingjing Zhang

{"title":"Recent Advances in Aptamer-Based Sensors for In Vitro Detection of Small Molecules","authors":"Yueru Mu, Zhenzhen Chen, Jiayin Zhan, Prof. Jingjing Zhang","doi":"10.1002/anse.202400027","DOIUrl":"https://doi.org/10.1002/anse.202400027","url":null,"abstract":"Sensitive and accurate detection of small molecules from complex matrix has aroused increasing interest in many fields, yet remains an open challenge. Recent years have witnessed a considerable advance of aptasensors for diagnostic assay development towards diverse small molecules because aptamer is one of the most powerful classes of molecular receptors with advanced affinity and specificity. Herein, we reviewed the small-molecule aptasensors in the past five years, focusing on the principles to specific applications in clinical diagnosis, food safety, and environmental monitoring. The first introductory section on the development of aptasensors in historical view and its analytical features contextualizes essential health-related small molecules. The second part highlights the basic components of aptasensor and the detection principles of different sensors based on signal output modes. The subsequent part systematically discusses various small-molecule sensing platforms by interfacing aptamers with diverse signal amplification strategies. Finally, challenges and perspectives for improving the aptasensor performance are also discussed. By describing biochemical and analytical procedures, this review highlights the optimal use of aptamers in the detection, quantification, and imaging of important health-related small molecules and presents new insights, technical advances, and engineering strategies for practical applications.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400027","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-Time Non-Faradaic Potentiodynamic Impedance Sensing Using Screen-Printed Carbon Electrodes 使用丝网印刷碳电极进行实时非法拉第电位阻抗检测

IF 3.4

Analysis & sensing Pub Date : 2024-06-18 DOI: 10.1002/anse.202400037

Emil Fuhry, Victoria Guglielmotti, Isabell Wachta, Diego Pallarola, Kannan Balasubramanian

{"title":"Real-Time Non-Faradaic Potentiodynamic Impedance Sensing Using Screen-Printed Carbon Electrodes","authors":"Emil Fuhry, Victoria Guglielmotti, Isabell Wachta, Diego Pallarola, Kannan Balasubramanian","doi":"10.1002/anse.202400037","DOIUrl":"https://doi.org/10.1002/anse.202400037","url":null,"abstract":"Electrochemical impedance spectroscopy (EIS) is a suitable analytical technique to detect interfacial phenomena and analyte binding at electrode surfaces. In contrast to metallic electrodes, carbon-based electrodes are more suited due to the low cost and the availability of more versatile methods for chemical functionalization. For (bio) sensing, often the Faradaic version of EIS in a three-electrode configuration is used, where a redox-active species is used as a marker. In order to avoid interference due to the redox-active marker with the interfacial interaction, we focus here on the use of non-Faradaic EIS in the absence of any added markers. First, we utilize the sedimentation of silica beads as a model system, which reduces the complexity of the interaction simplifying the interpretation of the measured signals. Moreover, we introduce two improvements. First, impedance measurements are performed in a three-electrode configuration with applied potential as an additional variable, which serves as a handle to optimize the sensitivity. Secondly, we present a time-differential strategy to detect subtle changes and demonstrate that we can consistently follow the sedimentation of beads using the non-Faradaic impedance as a function of the applied potential. Finally, we show a proof-of-principle demonstration for the biosensing of cell attachment on the electrodes in real-time using the proposed technique.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142642188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Progress in CO Detection Probes Based on Different Fluorophores 基于不同荧光团的 CO 检测探针的最新进展

IF 3.4

Analysis & sensing Pub Date : 2024-06-11 DOI: 10.1002/anse.202400021

Dr. Xinkuo Fang, Dr. Pengcheng Jiang, Prof. Haitao Yu, Dr. Yanyu Qi

引用次数: 0

Hybrid Paper/Polyester-Based Laser-Induced Graphene Electrodes for Electrochemical Detection of Tadalafil 用于电化学检测他达拉非的激光诱导石墨烯混合纸/聚酯电极

IF 3.4

Analysis & sensing Pub Date : 2024-06-11 DOI: 10.1002/anse.202400016

Shellyda G. G. Souza, Habdias A. Silva-Neto, Danielly S. Rocha, Abner de Siervo, Thiago R. L. C. Paixão, Wendell K. T. Coltro

{"title":"Hybrid Paper/Polyester-Based Laser-Induced Graphene Electrodes for Electrochemical Detection of Tadalafil","authors":"Shellyda G. G. Souza, Habdias A. Silva-Neto, Danielly S. Rocha, Abner de Siervo, Thiago R. L. C. Paixão, Wendell K. T. Coltro","doi":"10.1002/anse.202400016","DOIUrl":"10.1002/anse.202400016","url":null,"abstract":"Here, we describe for the first time the fabrication of laser-induced graphene (LIG) electrodes on a hybrid substrate composed of sandpaper and polyester. As a proof of concept, the proposed device was used as a voltammetric sensor for tadalafil (TAD) quantification in authentic tablet samples. The electrochemical TAD sensing based on differential pulse voltammetry (DPV) revealed a linear behavior in the concentration range from 25 to 250 μmol L−1(R2=0.99), a limit of detection of ~9.6 μmol L−1, sensitivity of ~0.0048 μA(μmol L−1)−1 and acceptable reproducibility values (RSD≤5.8 %). The DPV responses involving the standard addition method in pharmaceutical samples presented recovery results of TAD ranging from 93 to 108 %. Also, the proposed analytical method offered a suitable green analytical chemistry profile. We successfully demonstrated the fabrication of graphene-like sites and nanoparticles composed of alumina upon a hybrid substrate.","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":"4 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202400016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141356805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0