Analysis & sensing最新文献

{"title":"Electrochemical Biosensor Arrays for Multiple Analyte Detection","authors":"Debashis Sen,&nbsp;Prof. Dr. Robert A. Lazenby","doi":"10.1002/anse.202300047","DOIUrl":"10.1002/anse.202300047","url":null,"abstract":"<p>The simultaneous detection of multiple analytes using electrochemical biosensor arrays has demonstrated significant potential in various fields, including medical diagnostics. These platforms are designed to be low-cost, easy to use, and offer fast detection of a range of molecules. This concept article discusses the components necessary to achieve multiplexing with electrochemical biosensor arrays. The various methods used to fabricate electrode arrays of different types are discussed. Furthermore, the methods for the selective immobilization of multiple different bioreceptors onto individual electrodes within the array, a crucial step essential for conferring specificity to the analytical processes, are presented with relevant examples. We have focused on enzymes, antibodies and aptamers as examples of bioreceptors that have been deployed in various multianalyte electrochemical detection platforms. Finally, we discuss the key challenges associated with their application for the analysis of real samples and provide a future outlook on possible strategies that can be used to overcome these challenges.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135343846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent Probes for the Detection of Bone Metabolism","authors":"Jiawei Zhang,&nbsp;Sheng Pan,&nbsp;Bin Song,&nbsp;Prof. Youjia Xu,&nbsp;Prof. Houyu Wang,&nbsp;Prof. Yao He","doi":"10.1002/anse.202300057","DOIUrl":"10.1002/anse.202300057","url":null,"abstract":"<p>The skeleton plays a significant role in human health. Comprehensive and non-invasive visualization of the bone is in high demand to detect bone-related diseases. Clinically, conventional imaging techniques continue to face challenges in terms of limited imaging sensitivity, extended acquisition durations, and the inherent presence of ionizing radiation. These factors collectively hinder their capacity to furnish real-time spatial insights into cellular activity. Recently, Numerous innovative imaging methodologies employing diverse types of probes have emerged to enhance the clinical detection of bone. In vivo imaging of bone contribute to continuously detecting bone metabolism and growth, diagnosing bone metastases, visualizing medication delivery to bones. Fluorescent probes in dynamic detection of bone metabolism several inherent advantages. First, Fluorescent probes present lower potential long-term toxicity than radioactive isotope labels. Second, fluorescent dyes used in in vitro imaging are more cost-effective and come in a more compact size. Next, Near-infrared (NIR) dyes have deeper tissue penetration capability and lower tissue autofluorescence. However, bone-imaging fluorescent probes highly depend on phosphonate-related ligands. These ligands have multiple side effects in clinic and long half-live of them also exacerbates clearance concerns. This review aims to conclude and analyse the recently reported fluorescent probes for the precise detection of bone. Firstly, we outline the fundamental design mechanism of bone fluorescent probes and describe diverse bone-targeting moieties, emphasizing the targeting ligands, signal moieties, and functionalities of these probes. Secondly, we discuss the recent promising bone fluorescent probes for the precise and sensitive detection of bone. Finally, we offer our insights on potential future advancements in this field. We anticipate that this review will inspire creative ideas for designing and creating innovative bone-targeting probes, with applications in bone imaging, pharmaceutical screening, and assessing therapeutic outcomes.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135014575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Readily Available Red-Emitting Methylthio-Substituted Salicylaldehyde Azine with AIE Feature for Ratiometric Detection of HClO","authors":"Jingjun Zhao,&nbsp;Penglei Zhai,&nbsp;Dr. Biao Gu,&nbsp;Prof. Siping Tang","doi":"10.1002/anse.202300050","DOIUrl":"10.1002/anse.202300050","url":null,"abstract":"<p>Developing a simple, selective, and rapid reliable method to monitor hypochlorous acid (HClO) in vivo is very meaningful due to its important physiological and pathological functions. In this research, a novel AIE based fluorescent probe for ratiometric detection of HClO was fabricated by a simple one-step synthesis, in which the methyl sulfide group and salicylaldehyde azine serve as the recognition unit and fluorophore, respectively. This probe was visible-light-excitable, and showed a palpable aggregation-induced emission into the red region with high stability to Cu²⁺ and pH. Oxidation of the methyl sulfide group can be achieved rapidly and specifically by HClO in physiological condition, which enables it to display a rapid, ratiometric fluorescent response to HClO with high selectivity over other biologically pertinent species. By exploiting the probe-based tool, we successfully validated its practical utility in selectively recognizing HClO in living cells via ratiometric fluorescence signals, thereby providing a potential method for investigating the relevant functions of HClO in biosystems.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125451427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Peripheral Nerve Imaging by Fluorescent Polymer Dots for Minimizing the Risk of Intraoperative Nerve Injury","authors":"Juxiang Zhang,&nbsp;Shuting Lu,&nbsp;Shiyi Tang,&nbsp;Yuqiao Li,&nbsp;Yufan Zhang,&nbsp;Jingru Li,&nbsp;Liqin Xiong","doi":"10.1002/anse.202300042","DOIUrl":"10.1002/anse.202300042","url":null,"abstract":"<p>Iatrogenic nerve injury is an important cause of surgical complications. We focused on improving intraoperative visualization of nerves through highly fluorescent polymer dots (Pdots) and in vivo optical imaging instrumentation. The prepared Pdots have good biocompatibility, fluorescence stability, and simple preparation. Peripheral nerves can be imaged quickly with Pdots through a simple direct administration method and process, low non-specific absorption in the surrounding tissues. Moreover, the Pdots does not invade nerve cells but binds stably in the neural membrane structure. This study verifies that fluorescent Pdots have excellent neuroimaging capabilities and can be used for intraoperative visualization of peripheral nerves.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124044131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Metal-Organic Frameworks in Imaging-Guided Therapy","authors":"Dr. Man Zhu,&nbsp;Ming Ke,&nbsp;Wenjing Zhao,&nbsp;Qingqing Wu,&nbsp;Prof. Dr. Shaoguang Li,&nbsp;Prof. Dr. Hui Li,&nbsp;Prof. Dr. Fan Xia","doi":"10.1002/anse.202300052","DOIUrl":"10.1002/anse.202300052","url":null,"abstract":"<p>Metal-organic frameworks (MOFs) are formed by the self-assembly of metal centres/clusters and organic ligands. A diverse range of organic ligands can be employed, including nucleobases, amino acids, peptides, proteins, and saccharides <i>et al</i>. Due to the characteristics of large surface area, high porosity, easy surface functionalization and adjustable internal pore size, <i>et al</i>. MOFs have been used as a significant emerging class of biological imaging agents and have attracted great research interest in optical imaging (OI), photoacoustic imaging (PAI), magnetic resonance imaging (MRI), computer tomography (CT) and positron emission tomography (PET) in recent years. This review provides a comprehensive overview of the diverse range of organic ligands utilized in Metal-organic frameworks, while also delving into the recent advancements, opportunities, and challenges encountered in the realm of bioimaging in recent years.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129360671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Nanozymes in Biomedical Imaging","authors":"Dr. Guotao Yuan,&nbsp;Yiyin Mai,&nbsp;Jieyao Chen,&nbsp;Prof. Yichu Nie,&nbsp;Prof. Chong Wang,&nbsp;Prof. Qinghua Liu,&nbsp;Prof. Xin Tian,&nbsp;Prof. Yue Pan","doi":"10.1002/anse.202300048","DOIUrl":"10.1002/anse.202300048","url":null,"abstract":"<p>Nanozymes are a type of artificial enzyme that possess both the unique properties and catalytic activities of nanomaterials. With the rapid development of nanotechnology and biomedicine, nanozymes provide potential opportunities for biomedical applications. In particular, nanozymes, combined with their unique physicochemical performance and enzymatic activity, have been extensively used for <i>in vitro</i> sensing and <i>in vivo</i> imaging. In this review, we systematically summarized the progress of nanozymes and their applications in <i>in vivo</i> imaging and finally proposed the challenges and prospects in the development of nanozymes in biomedical imaging.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125615796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Lucigenin Assay: Measuring Anion Transport in Lipid Vesicles**","authors":"Matúš Chvojka,&nbsp;Anurag Singh,&nbsp;Alessio Cataldo,&nbsp;Dr. Aaron Torres-Huerta,&nbsp;Dr. Marcin Konopka,&nbsp;Prof. Vladimír Šindelář,&nbsp;Dr. Hennie Valkenier","doi":"10.1002/anse.202300044","DOIUrl":"10.1002/anse.202300044","url":null,"abstract":"<p>Synthetic anion transporters are developed to transport anions across lipid membranes with the long-term perspective of biological applications. The lucigenin assay is a popular tool to study their transport of chloride and other anions in liposomes. It relies on the quenching of the fluorescence of encapsulated lucigenin by anions, which can be monitored by fluorescence spectroscopy. This article provides a tutorial introduction to the practical use and understanding of the lucigenin assay. It describes in detail how to use this assay to monitor chloride/nitrate antiport in liposomes, process and interpret the data, and solve common issues. Variations of the assay enabling the investigation of the transport of other anions and transport mechanisms are discussed. Furthermore, a zwitterionic analogue of lucigenin is demonstrated to have advantages for use in experiments over longer time scales, as it does not leak out of the liposomes, or when studying chloride uniport, as it avoids interference from antiport with nitrate that is present in commercial lucigenin.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115655622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Glutathione-Responsive Luminescence Sensor Based on Dual-Emissive Persistent Luminescent Nanoparticles for Ratiometric Tumor Imaging","authors":"Xue-Mei Gao,&nbsp;Tian-Yue Gu,&nbsp;Ke-Lin Chen,&nbsp;Dr. Xu Zhao,&nbsp;Prof. Xiu-Ping Yan","doi":"10.1002/anse.202300045","DOIUrl":"10.1002/anse.202300045","url":null,"abstract":"<p>Persistent luminescent nanoparticles (PLNP) have outstanding advantages in low-background imaging. However, avoiding exogenous interference and false positive signals to achieve precise imaging is still a problem. In addition, it is also a great challenge to intelligently control the size of PLNP. Herein, we report an intelligent luminescence ratiometric sensor based on ultra-small dual-emissive PLNP for precise tumor-targeted imaging. ZnGa₂O₄:Cr PLNP with a particle size of c.a. 5–10 nm and two emission peaks at 708 nm and 501 nm were firstly synthesized by thermal decomposition method along with systematical controlling the amount of chromium. [email protected] sensor was further constructed with a constant ratio (<i>I</i>₇₀₈/<i>I</i>₅₀₁) which is not interfered by exogenous factors such as detection time window and probe concentration. However, high concentrations of glutathione in the tumor microenvironment can specifically trigger the change of <i>I</i>₇₀₈/<i>I</i>₅₀₁ and cause the sensor to self-assemble into clusters at tumor site, thus realizing long-term retention and specificity imaging.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133036572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological Transformation of Self-Assembled Peptide Nanostructures for Bioimaging Applications","authors":"Ying Xu,&nbsp;Qiaochu Jiang,&nbsp;Dr. Xianbao Sun,&nbsp;Prof. Gaolin Liang","doi":"10.1002/anse.202300039","DOIUrl":"10.1002/anse.202300039","url":null,"abstract":"<p>Stimuli-triggered in-situ morphological transformation of peptide nanomaterials may enhance the on-site accumulation and retention of the imaging agent cargos in the stimuli-rich regions, thus enabling precise, sensitive, and prolonged imaging of diseases. Moreover, this strategy permits the co-delivery of contrast agents and drugs with the smart “turn-on” ability, allowing for efficient disease theranostics. In light of the significance of this strategy in designing smart biomedical peptide materials, which remains scarcely reviewed in recent years, we herein provide this review. We summarize the bioimaging applications (i. e., fluorescence imaging, magnetic resonance imaging, and photoacoustic imaging) of these smart morphological transformation-based peptide materials, and highlight the remarkable breakthroughs. Besides, challenges to be addressed in this field are discussed.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116802178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arylamino-substituted Rhodamine as a Fluorogenic Molecular Rotor for the Wash-free Imaging of Non-catalytic Proteins in Live Cells","authors":"Huiling Tang,&nbsp;Xia Yuan,&nbsp;Yefeng Chen,&nbsp;Dr. Yuyao Li,&nbsp;Prof. Dr. Xiaoyong Xu,&nbsp;Prof. Dr. Hexin Xie","doi":"10.1002/anse.202300037","DOIUrl":"10.1002/anse.202300037","url":null,"abstract":"<p>Fluorescent probes are valuable tools to visualize non-catalytic proteins in live cells. Currently, the majority of imaging reagents for non-catalytic proteins are based on “always-on” fluorophores and the use of these reagents usually necessitate a wash step to remove unbounded fluorophores before microscope imaging. Herein, we report the use of arylamino-substituted rhodamine as an activatable fluorophore for the imaging of non-catalytic protein in live cells. We have shown the induction of an arylamino to structurally rigid rhodamine could significantly reduce the fluorescent emission in aqueous medium but the ligand-directed binding of this molecule to protein receptor could effective restrict its intramolecular motion and thus lead to enhancement in fluorescence intensity at 590 nm over 30-fold. With fluorescent probes based on this fluorophore, we could visualize integrin <i>α_vβ₃</i> and azido-functionalized glycans in living cells with high contrast in a wash-free manner.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124448192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}