{"title":"Translating Imaging Nanoprobes Design to the Clinic","authors":"Wenbo Sun, Geng Tian, Prof. Dr. Lehui Lu","doi":"10.1002/anse.202300038","DOIUrl":"https://doi.org/10.1002/anse.202300038","url":null,"abstract":"<p>The use of nanoprobes has revolutionized the way we approach medical diagnosis and treatment. These tiny particles are designed to target specific cells or tissues within the body, allowing doctors to visualize and monitor disease progression in real-time. The paramount consideration in the utilization of nanoprobes is their safety. Unlike traditional contrast agents, which can cause adverse reactions in some patients, these probes are specifically engineered to minimize any potential harm. And it can promote the rapid development of new biological imaging techniques in various fields such as biological structure and functional imaging, disease diagnosis, in situ imaging, and real-time dynamic imaging at the in vivo level. Based on the principles and mechanisms of imaging, this study focuses on the recent applications of nanoprobes for several typical imaging techniques (magnetic resonance imaging, computed tomography imaging, fluorescence imaging, and acoustic imaging) with the aim of providing researchers with a fresh perspective on precise disease diagnosis and treatment through the development of nanoprobes.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"109168887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr. Ming-Hao Liu, Ning-Ning Zhao, Wan-Tong Yu, Jin-Zhi Zhang, Prof. Zi-Yue Wang, Prof. Chun-Yang Zhang
{"title":"Construction of an Oxidative Cleavage-Activated DNAzyme Biosensor for Rapid Detection and Cellular Imaging of the Myeloperoxidase Activity","authors":"Dr. Ming-Hao Liu, Ning-Ning Zhao, Wan-Tong Yu, Jin-Zhi Zhang, Prof. Zi-Yue Wang, Prof. Chun-Yang Zhang","doi":"10.1002/anse.202300043","DOIUrl":"10.1002/anse.202300043","url":null,"abstract":"<p>Myeloperoxidase (MPO) is a mammalian pro-oxidant protease and it is closely related to severe infections and diverse inflammatory diseases. Rapid and sensitive measurement of MPO activity is essential for anticancer drug discovery and inflammatory research. Herein, we demonstrate the construction of an oxidative cleavage-activated deoxyribozymes (DNAzyme) biosensor for rapid detection and cellular imaging of the MPO activity. When target MPO is present, the phosphorothioate (PS)-modified hairpin probe is site-specifically cleaved by MPO, releasing the intact Mg<sup>2+</sup>-dependent DNAzyme sequences. Subsequently, the activated DNAzyme initiates the cyclic cleavage of the signal probe with the assistance of cofactor Mg<sup>2+</sup>, liberating large numbers of Cy5 molecules. This assay possesses the characteristics of easy operation, low sample consumption, without the requirements of expensive radiolabeling, antibodies, and nanomaterials. Especially, this assay can be performed in one pot under isothermal conditions (37°C) within 60 min. Due to the high efficiency of DNAzyme-based cyclic cleavage reaction and the intrinsic advantages of single-molecule detection, this assay achieves high sensitivity with a limit of detection (LOD) of 2.74×10<sup>−3</sup> ng μL<sup>−1</sup>. It can be applied to screen MPO inhibitors, measure cellular MPO activity at the single-cell level, and image intracellular MPO in living cells, providing a powerful platform for early clinical diagnosis and drug discovery.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116422564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr. Jianping Zhu, Nian Kee Tan, Kai Kikuchi, Dr. Amandeep Kaur, Prof. Elizabeth J. New
{"title":"BODIPY-based Fluorescent Indicators for Lipid Droplets","authors":"Dr. Jianping Zhu, Nian Kee Tan, Kai Kikuchi, Dr. Amandeep Kaur, Prof. Elizabeth J. New","doi":"10.1002/anse.202300049","DOIUrl":"10.1002/anse.202300049","url":null,"abstract":"<p>Lipid droplets are essential for cellular lipid storage, playing critical roles in cellular lipid metabolism. Although lipid droplets have drawn intense research in recent years, much remains to be uncovered about the roles of this organelle in biology. Lipid droplet indicators exhibiting large Stokes shifts and improved brightness are therefore in demand. We report two asymmetric BODIPY derivatives, <b>BoL1</b> and <b>BoL2</b> bearing a benzothiazole group at the 6-position, which led to bathochromic shifts of 62 nm for <b>BoL1</b> and 37 nm for <b>BoL2</b> in fluorescence emission when compared to the reference molecules without the benzothiazole group. The incorporation of the benzothiazole moiety also resulted in a large Stokes shift of 40 nm. <b>BoL1</b> and <b>BoL2</b> have been demonstrated to operate as lipid droplet indicators in both confocal and STED imaging. <b>BoL2</b> showed particularly good cellular retention and was further applied to explore the impact of cellular starvation on the trafficking of lipid droplets.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124467052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoxiao Jin, Yikang Wu, Jiannan Sun, Prof. Jinhua Liu, Prof. Heyong Cheng
{"title":"Cover Feature: Catalyst-Free Three-Component Petasis Reactions Accelerated in Microdroplets: Reaction Optimization and Senstive Detection by Mass Spectrometry (Anal. Sens. 6/2023)","authors":"Xiaoxiao Jin, Yikang Wu, Jiannan Sun, Prof. Jinhua Liu, Prof. Heyong Cheng","doi":"10.1002/anse.202300046","DOIUrl":"https://doi.org/10.1002/anse.202300046","url":null,"abstract":"<p><b>The cover feature image illustrates</b> two mail devices, drones (representing sprayed microdroplets) and in-person (representing bulk phase), to deliver a letter (representing the Petasis reaction involving aldehydes, arylboronic acids and amines) in sharply different efficiencies. This study supplies not only a mild, efficient and environmentally friendly methodology to constructing aryl amines in organic community but also a useful derivatization strategy for highly sensitive mass spectrometric detection of arylboronic acids and aryl aldehydes. More information can be found in the Research Article by Heyong Cheng, Jiannan Sun, and co-workers.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"109232520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr. Yui Sasaki, Xiaojun Lyu, Prof. Tsuyoshi Minami
{"title":"Front Cover: A Highly Accurate pH Detection Method for Sweat Analysis using a Printed 96-Microwell Colorimetric Sensor Array (Anal. Sens. 5/2023)","authors":"Dr. Yui Sasaki, Xiaojun Lyu, Prof. Tsuyoshi Minami","doi":"10.1002/anse.202300035","DOIUrl":"https://doi.org/10.1002/anse.202300035","url":null,"abstract":"<p><b>The cover picture shows</b> a printed 96-microwell paper-based sensor array depicting various color patterns upon changing pH conditions, which enables the prediction of pH values below the decimal point in human sweat by imaging analysis and pattern recognition techniques. More information can be found in the Research Article by Yui Sasaki, Xiaojun Lyu, and Tsuyoshi Minami .\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50123924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr. Yui Sasaki, Xiaojun Lyu, Prof. Tsuyoshi Minami
{"title":"A Highly Accurate pH Detection Method for Sweat Analysis using a Printed 96-Microwell Colorimetric Sensor Array","authors":"Dr. Yui Sasaki, Xiaojun Lyu, Prof. Tsuyoshi Minami","doi":"10.1002/anse.202300036","DOIUrl":"https://doi.org/10.1002/anse.202300036","url":null,"abstract":"<p>Invited for this month's cover are is the collaborating group of Prof. Minami at The University of Tokyo. The cover picture shows a printed 96-microwell paper-based colorimetric sensor array to detect pH changes below the decimal point in human sweat. More information can be found in the Research Article by Yui Sasaki, Xiaojun Lyu, and Tsuyoshi Minami.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50123923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaoxiao Jin, Yikang Wu, Jiannan Sun, Prof. Jinhua Liu, Prof. Heyong Cheng
{"title":"Catalyst-Free Three-Component Petasis Reactions Accelerated in Microdroplets: Reaction Optimization and Senstive Detection by Mass Spectrometry","authors":"Xiaoxiao Jin, Yikang Wu, Jiannan Sun, Prof. Jinhua Liu, Prof. Heyong Cheng","doi":"10.1002/anse.202300031","DOIUrl":"https://doi.org/10.1002/anse.202300031","url":null,"abstract":"<p>Due to their important roles in medicine, the product of the Petasis reaction has attracted extensive interest in pharmaceutical, medical and chemical communities. Traditional three-component Petasis methods normally use various catalysts under harsh conditions (high temperature, microwave, etc.) for long reaction times. In this study, we developed a green and highly efficient microdroplet method for accelerating the Petasis reaction, which obtain good yields without the need of any catalysts under mild reaction conditions. The Petasis reaction in microdroplets was suitable for a variety of salicylaldehydes, arylboronic acids and amines. The Petasis reaction in microdroplets was accelerated by approximately 4 orders of magnitude by comparing the measured rate constants in bulk. Further, a scaled-up amount of 0.8 g h<sup>−1</sup> was achieved for the Petasis reaction in microdroplets. This study supplies not only a high-efficiency and environment-friendly methodology to constructing aryl amines in organic community but also a useful derivatization strategy for highly sensitive mass spectrometric detection of arylboronic acids and aryl aldehydes.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"109163378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How to Develop Bioresponsive MRI Probes Based on Paramagnetic Gd(III) for in vivo Applications","authors":"Ping Yue, Dr. Goran Angelovski","doi":"10.1002/anse.202300019","DOIUrl":"https://doi.org/10.1002/anse.202300019","url":null,"abstract":"<p>Among the many biological imaging techniques, magnetic resonance imaging (MRI) has been widely adopted for biomedical and clinical diagnostic applications, because of its ability to image deep tissues with high spatiotemporal resolution. Bioresponsive contrast agents are the key to expanding the diagnostic potential of MRI by providing anatomical information and discerning biochemical activity. Recent developments in the field of responsive and gadolinium-based agents have resulted in novel complexes that can sense their chemical microenvironments and thus study various functional processes in the tissue. Herein, we discuss the design and use of Gd(III)-based and bioresponsive MRI contrast agents for specific biological markers such as Ca(II) and Zn(II) cations and zwitterionic amino acid neurotransmitters. Combining their basic physicochemical characteristics with aspects that should be considered for their use in vivo would achieve the desired sensing features and enable their applications in functional molecular imaging to visualize essential biological processes.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"109170748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dr. Zijie Zhang, Dr. Jiuxing Li, Ryan Amini, Alexandria Mansfield, Jimmy Gu, Jianrun Xia, Prof. John D. Brennan, Prof. Yingfu Li
{"title":"Cover Feature: Comparative Characterization of Diverse DNA Aptamers for Recognition of Spike Proteins of Multiple SARS-CoV-2 Variants (Anal. Sens. 5/2023)","authors":"Dr. Zijie Zhang, Dr. Jiuxing Li, Ryan Amini, Alexandria Mansfield, Jimmy Gu, Jianrun Xia, Prof. John D. Brennan, Prof. Yingfu Li","doi":"10.1002/anse.202300029","DOIUrl":"https://doi.org/10.1002/anse.202300029","url":null,"abstract":"<p><b>The cover image illustrates</b> the binding of a group of DNA aptamers selected to recognize the spike protein (S-protein) of SARS-CoV-2, the virus that causes COVID-19. The binding affinity for several key variants of the S-protein and the degree of overlapping of the binding sites of the aptamers on the S-protein have been comparatively examined. The design was created with Biorender.com. More information can be found in the Research Article by John D. Brennan, Yingfu Li, and co-workers.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300029","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50125159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yupu Zhang, Dr. Xinfeng Du, Dr. Jingying Zhai, Prof. Xiaojiang Xie
{"title":"Front Cover: A Tunable Colorimetric Carbon Dioxide Sensor Based on Ion-Exchanger- and Chromoionophore-Doped Hydrogel (Anal. Sens. 6/2023)","authors":"Yupu Zhang, Dr. Xinfeng Du, Dr. Jingying Zhai, Prof. Xiaojiang Xie","doi":"10.1002/anse.202300032","DOIUrl":"https://doi.org/10.1002/anse.202300032","url":null,"abstract":"<p><b>The cover feature image shows</b> a two-dimensional (2D) colorimetric carbon dioxide (CO<sub>2</sub>) sensor composed of a gas-permeable polypropylene film (25 μm thick) and a signal transduction hydrogel layer (30 μm thick). The hydrogel layer contained a pH sensitive chromoionophore to indicate the CO<sub>2</sub> induced pH change, and a cationic amine to further capture CO<sub>2</sub> through the carbamate formation reaction. With this 2D colorimetric CO<sub>2</sub> optode, the CO<sub>2</sub> release from yeast-catalyzed flour fermentation was successfully monitored. More information can be found in the Research Article by Jingying Zhai, Xiaojiang Xie, and co-workers.\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/anse.202300032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"109165675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}