利用微液滴加速衍生化反应的二维串联质谱法快速鉴定混合物中的酚类物质

IF 3.4 Q2 CHEMISTRY, ANALYTICAL
Yanyang Hu, Dr. Eric T. Dziekonski, Donna M. Wang, Dr. L. Edwin Gonzalez, Prof. R. Graham Cooks
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引用次数: 0

摘要

复杂混合物分析通常采用色谱分离法,然后再用质谱法进行分析。二维串联质谱法(2D MS/MS)无需进行分离,因此可大大缩短分析时间,同时还能记录所有捕获的前体离子产生的所有碎片离子的强度。通过化学衍生和重复二维 MS/MS 扫描,还可提供额外的化学特异性。在本研究中,通过观察前体离子轴上 175 Da 的质量移动和二维 MS/MS 数据域中 119 Da 的中性损失 (NL),对三唑点击衍生后的酚类化合物进行了鉴定。衍生反应在带电电喷雾微滴中加速进行。使用这种方法,可以在 1-2 分钟内鉴定出这一类化合物的成员,同时获得单个化合物的结构信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Rapid Identification of Phenolics in Mixtures by Two-Dimensional Tandem Mass Spectrometry with Microdroplet Accelerated Derivatization Reactions

Rapid Identification of Phenolics in Mixtures by Two-Dimensional Tandem Mass Spectrometry with Microdroplet Accelerated Derivatization Reactions

Complex mixtures are typically analyzed by chromatographic separation followed by mass spectrometry. Two-dimensional tandem mass spectrometry (2D MS/MS) may eliminate the need for separation and therefore significantly reduce analysis time while expanding the information space by recording the intensities of all fragment ions generated from all trapped precursor ions. Additional chemical specificity can be provided by using chemical derivatization. In this study phenolic compounds are identified after triazole click derivatization by the observation of two diagnostic features in the 2D MS/MS data domain, a mass shift of 175 Da on the precursor ion axis and a neutral loss (NL) of 119 Da. Additional NL transitions were highly correlated with particular substituents including carbonyl, ester, amino, and alkyl/phenyl groups. Substituent position information was also easily diagnosed. The derivatization reactions are accelerated in the charged electrosprayed microdroplets. Using this method, members of this single class of compounds can be identified within 1–2 minutes while structural information on the individual compounds is acquired simultaneously.

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