基于表达嗅觉受体细胞的气味生物传感器:最新进展

IF 3.4 Q2 CHEMISTRY, ANALYTICAL
Hongchao Deng, Takamichi Nakamoto
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引用次数: 0

摘要

受到强大的生物嗅觉的启发,科学家们从动物嗅觉系统中提取了嗅觉感觉神经元、嗅觉受体(OR)蛋白和气味结合蛋白等多种材料,然后将它们与传感器相结合,形成了多种气味生物传感器。这些生物传感器虽然很好地继承了生物的传感能力,但也存在一些缺点,如制备过程复杂、传感材料特性不稳定、成本高昂等。与上述生物材料不同,表达异源 OR 的细胞经过多代传代后仍能保持稳定的传感性能,而且实验操作简单、成本低。因此,基于细胞表达 OR 的气味生物传感器近年来得到了很好的发展。在这篇综述中,我们首先列举了几种基于细胞表达 OR 的气味生物传感器,主要集中在荧光和电生理测量方法上。此外,我们还介绍了提高生物传感器性能的技术,如更宽的检测范围、更长的寿命、更多的 OR 类型和更高的定量效率。此外,我们还解释了大型传感器阵列和预测气味反应等可能的前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

Odor Biosensors Based on Cell Expressing Olfactory Receptor: Recent Advances

Inspired by the powerful biological olfaction, scientists extracted numerous materials such as olfactory sensory neuron, olfactory receptor (OR) protein, and odorant binding protein from animal olfactory systems, then combined them with transducers to form multiple odor biosensors. These biosensors, despite well inheriting the sensing ability of creatures, have several drawbacks, such as complex preparation process, unstable sensing material characteristics, and high cost. Unlike the biological materials listed above, cell expressing heterologous OR maintains a stable sensing performance after passaging for multiple generations, also its experimental operation is simple, and cost is low. Therefore, odor biosensors based on cell expressing OR have been well developed in recent years. In this review, we first listed several odor biosensors based on cell expressing OR, mainly focusing on fluorescent and electrophysiological measurement methods. Furthermore, we illustrated the techniques to improve the biosensor performance, e. g., wider detection range, longer lifetime, more OR types, and higher quantification efficiency. In addition, we explained the possible prospects such as big sensor array and predicting odor response.

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