Aggregate (Hoboken, N.J.)最新文献

{"title":"Supramolecularly Crosslinked Self-Healable Polymer Hydrogels for Adaptive Energy Dissipation and Fragile-System Protection","authors":"Dineshkumar Bharathidasan,&nbsp;Chandan Maity","doi":"10.1002/agt2.70412","DOIUrl":"https://doi.org/10.1002/agt2.70412","url":null,"abstract":"<p>Hydrogels with rapid mechanical energy-dissipation capability are highly desirable for protective and shock-absorbing applications. However, conventional systems often suffer from irreversible network damage, slow recovery, and mechanical fatigue. Herein, we report a biodegradable supramolecularly crosslinked polymer hydrogel (<b>AGK</b>) engineered through the synergistic integration of boronate ester covalent networks and G4-quartet supramolecular assemblies. The coupled covalent–supramolecular interactions promote reversible network dissociation and adaptive molecular reorganization under mechanical stress, enabling rapid self-healing, enhanced structural integrity, and efficient energy dissipation. The <b>AGK</b> hydrogel withstands compressive loads up to ∼5.4 kg, significantly outperforming the pristine biopolymer hydrogel (∼2.9 kg). Dynamic impact analyses reveal a remarkably low coefficient of restitution (∼0.21), substantially lower than those of conventional polymeric materials, demonstrating superior impact attenuation and rapid mechanical energy dissipation. Consequently, the hydrogel effectively protects fragile objects from both static compression and free-fall impact damage while maintaining mechanical resilience over repeated damage–recovery cycles. This work establishes a sustainable supramolecular engineering strategy for designing adaptive self-healable hydrogels with efficient energy-dissipation capability, offering promising opportunities for advanced protective, impact-mitigating, and mechanically adaptive soft materials.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helically Flexible Boron-Dipyrromethenes for Solution-Processed Isotropic Films With Preserved Chiral Exciton Coupling","authors":"Carolina Díaz-Norambuena,&nbsp;Juan Manuel Moreno-Naranjo,&nbsp;David Reger,&nbsp;Giuliano Siligardi,&nbsp;César Ray,&nbsp;Jessica Colligan,&nbsp;Florencio Moreno,&nbsp;Beatriz L. Maroto,&nbsp;Gilles Muller,&nbsp;Jorge Bañuelos,&nbsp;Matthew J. Fuchter,&nbsp;Santiago de la Moya","doi":"10.1002/agt2.70411","DOIUrl":"https://doi.org/10.1002/agt2.70411","url":null,"abstract":"<p>Chiral organic thin films are emerging as key materials in advanced optoelectronic and chiroptical technologies, where the straightforward, solution-processed fabrication of chiroptically active neat films from small π-conjugated helical molecules is highly desirable for sustainable and scalable device integration. However, in such densely packed systems, the intrinsic chiroptical response is often compromised by macroscopic anisotropies (e.g., linear dichroism and birefringence) and aggregation-induced effects, which obscure genuine molecular signatures and induce or alter chiral exciton coupling. Here, we demonstrate that a readily accessible and conformationally flexible helically chiral boron-dipyrromethene dimer (<i>helico</i>BODIPY) platform enables the reproducible fabrication of robust solution-processed neat thin films exhibiting a unique behavior: chiroptical isotropy while retaining the characteristic molecular chiroptical response arising from intramolecular chiral exciton coupling. Through systematic structural tuning, these systems exhibit enhanced electronic circular dichroism (ECD) and circularly polarized luminescence in solution. Remarkably, the sharp ECD couplets and absorption dissymmetry values measured in the corresponding films closely reproduce those observed in solution, indicating minimal contribution from anisotropic contributions and negligible perturbation of excitonic interactions in the condensed phase. These unique findings point to helically flexible <i>helico</i>BODIPYs as an excellent molecular platform that bridges molecular design and thin-film performance, offering a potential tool for disentangling aggregation effects in chiral thin films and advancing the rational design of chiral organic materials with predictable chiroptical properties.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Programmable Interfacial Energy Alignment in Plasmonic Metal-Organic Frameworks for Selective Molecular Probing on Complex Heterogeneous Surfaces","authors":"Yahui Zhang,&nbsp;Jiachang Chen,&nbsp;Xia Huang,&nbsp;Shiqi Zou,&nbsp;Xinzhan Cui,&nbsp;Xiaoyu Ma,&nbsp;He Li","doi":"10.1002/agt2.70403","DOIUrl":"https://doi.org/10.1002/agt2.70403","url":null,"abstract":"<p>In situ identification and spatially resolved analysis of multicomponent species on complex heterogeneous surfaces remain formidable challenges in surface science, advanced manufacturing, and cultural heritage conservation. Although surface-enhanced Raman scattering (SERS) provides highly sensitive molecular fingerprints, existing platforms often struggle to simultaneously achieve robust interfacial contact and discriminable responses on rough, porous, and compositionally heterogeneous solids. Here, we report a programmable interfacial energy-alignment strategy by integrating a core–shell plasmonic metal-organic framework (MOF) heterojunction, Fe₃O₄@SiO₂@UiO-66(X)@Ag triangular nanoplates (Ag TNs), into an injectable poly(vinyl alcohol) (PVA) microgel matrix to construct a self-adaptive SERS chip. The microgel fills surface asperities and establishes semipermeable conformal contact. Upon dehydration, it locally densifies interfacial nanogaps and reconstructs hotspot networks, thereby promoting more effective interfacial coupling within accessible pores and cracks while improving signal stability and reproducibility. Meanwhile, ligand engineering of UiO-66(X) modulates the interfacial electronic structure and favors phase-dependent charge-transfer-assisted enhancement, whereas tuning the tip sharpness of Ag TNs regulates localized surface plasmon resonance (LSPR) coupling under different excitation wavelengths. Excitation- and ligand-controlled SERS experiments, together with density functional theory calculations, provide an electronic-structure rationale for the observed preferential responses and support the involvement of cooperative electromagnetic and charge-transfer-assisted enhancement contributions. As a result, the chip enables phase-resolved identification and spatial mapping of corrosion products in model multilayer systems, as well as low-disturbance in situ analysis and interval-based corrosion-state estimation of excavated bronze artifacts. This work provides a general materials-design framework for programmable interfacial energy-level regulation toward molecular sensing and chemical imaging on complex solid surfaces.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor Screening and Stoichiometry Engineering-Driven Supramolecular Charge-Transfer Complexes for Synergistic Tumor Near-Infrared-II Phototherapy","authors":"Laiping Fang,&nbsp;Yike Tu,&nbsp;Shufang Li,&nbsp;Haiming Feng,&nbsp;Yu Cao,&nbsp;Wenqing Xiao,&nbsp;Jin Fang,&nbsp;Ping'an Ma,&nbsp;Wujun Geng,&nbsp;Guihua Jiang","doi":"10.1002/agt2.70419","DOIUrl":"https://doi.org/10.1002/agt2.70419","url":null,"abstract":"<p>The advancement of organic near-infrared-II (NIR-II) photothermal agents is impeded by complex structural designs and arduous synthetic procedures. While supramolecular charge-transfer complexes (CTCs) offer a facile assembly route, efficient NIR-II-absorbing CTCs remain scarce. Crucially, a fundamental paradox arises: tight planar stacking for narrow-bandgap NIR-II absorption inevitably induces severe aggregation-caused quenching (ACQ), suppressing reactive oxygen species generation and disabling synergistic photothermal/photodynamic therapy. Herein, we identified DBTTF (D₂) as the optimal electron donor through a systematic screening against a TFTQ acceptor. Significantly, the 2A-D₂ assembly with a 2-to-1 acceptor-to-donor ratio was established as the optimal candidate due to its highest molar extinction coefficient by deciphering the stoichiometry-property relationship. Molecular dynamics simulations revealed that this acceptor-rich environment induces looser molecular packing and weaker intermolecular interactions. This unique configuration successfully resolved the ACQ bottleneck, enabling efficient reactive oxygen species generation while maintaining a high photothermal conversion efficiency of 40.3%. Driven by these synergetic effects, 2A-D₂ induced severe mitochondrial dysfunction and DNA damage under 1064 nm irradiation, achieving robust 94.5% tumor inhibition and suppressing pulmonary metastasis. Thus, this study established a robust framework for developing advanced NIR-II CTCs by integrating donor screening with precise stoichiometric engineering to harmonize photothermal and photodynamic modalities for tumor ablation.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70419","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Plasmonic Biohybrid With an Intracellular Biogenic S-Scheme Nano-Heterojunction Toward Wide-Spectrum Solar-To-Chemical Conversion Beyond Nature","authors":"Heng Yu,&nbsp;Tingting Guo,&nbsp;Guanglei Ma,&nbsp;Xueqing Ren,&nbsp;Yue Zhang,&nbsp;Ruijuan Qi,&nbsp;Nana Ma,&nbsp;Meilin Wei,&nbsp;Yi Chang,&nbsp;Xiaoming Ma","doi":"10.1002/agt2.70415","DOIUrl":"https://doi.org/10.1002/agt2.70415","url":null,"abstract":"<p>Photosynthetic biohybrid systems (PBSs), which integrate microorganisms with inorganic semiconductors, offer a sustainable platform for solar-to-chemical conversion. However, most PBSs are hindered by the limited light absorption and inefficient charge separation of semiconductor. Herein, a biohybrid system featuring an intracellular in-situ synthesized CuS/CdS plasmonic nano-heterojunction (CuS/CdS@yeast) is developed for wide spectrum–driven photocatalysis. Regulated by the organism, biogenic CuS/CdS nano-heterojunctions achieve efficient electron-hole separation by forming an S-scheme heterostructure, driven by a substantial electrostatic potential difference. Furthermore, in-situ intracellular synthesis strategy enables a coupling between yeast cells and the nano-heterojunction, allowing photoelectrons of the semiconductor to directly participate in bioenergetic pathways, resulting in a significant elevation of intracellular reduction equivalents (nicotinamide adenine dinucleotide [NADH]) and available energy (adenine nucleoside triphosphate [ATP]), which are essential to sustain microbial biosynthesis. By synergizing a heterojunction with biological components, this biohybrid achieves superior photocatalytic reduction under full-spectrum illumination. Notably, it delivers an ammonia synthesis rate of 93.94 µmol·g⁻¹·h⁻¹ (without sacrificial agents) and a hydrogen production of 3803.47 µmol·g⁻¹, representing ∼13-fold and ∼27-fold enhancements over pure cells. This work presents novel insights into the rational design of efficient biohybrid systems for sustainable biomanufacturing.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70415","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148752809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting the Photocatalytic Performance of Layered Double Hydroxides by In Situ Reactions with Metal Nanoclusters","authors":"Qi Chen,&nbsp;Zhipu Zhang,&nbsp;Xinxin Pan,&nbsp;Manxi Zhang,&nbsp;Qiaofeng Yao,&nbsp;Wenping Hu","doi":"10.1002/agt2.70416","DOIUrl":"https://doi.org/10.1002/agt2.70416","url":null,"abstract":"<p>Layered double hydroxides (LDHs) are promising photocatalysts but suffer from wide bandgaps and rapid recombination of photogenerated charge carriers, resulting in poor visible-light activity. Conventional metal nanoparticle sensitization represents a useful means to improve the photocatalytic performance of LDHs, while its mechanism is scarcely understood at the atomic level. Herein, we report a sensitization strategy based on interfacial reactions between metal nanoclusters (NCs) and LDHs to enhance visible-light-driven photocatalysis. Au_29-43(SG)_27–37 NCs (SG denotes glutathione) were introduced into the regeneration process of MgAl-based layered double oxides (MgAl-LDOs), where strong electrostatic interactions between positively charged hydroxide layers and negatively charged carboxylate ligands induced cluster motif reconstruction and size conversion into Au₂₂(SG)₁₈ NCs. The resulting Au₂₂/LDH composites exhibit a significantly narrowed bandgap (2.21 eV), extended visible-light absorption up to 561 nm, and 4.3 times increase in specific surface area. These synergistic effects lead to markedly enhanced photocatalytic degradation of methylene blue, achieving 20.3% removal within 120 min under visible light. Radical scavenging experiments demonstrate that H₂O₂ and hydroxyl radicals (·OH) are the dominant reactive species in this system. This work demonstrates an effective route for LDHs sensitization and underscores the critical role of interfacial reconstruction in regulating photocatalytic performance.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70416","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self‑Protective Carbonized Polymer Dots Enabling Ultralong Room‑Temperature Phosphorescence in Strong and Tough Flexible Gels","authors":"Yulei Gao,&nbsp;Pingchuan Sun,&nbsp;Fenfen Wang","doi":"10.1002/agt2.70418","DOIUrl":"https://doi.org/10.1002/agt2.70418","url":null,"abstract":"<p>Constructing flexible ultralong room-temperature phosphorescence (URTP) materials is highly attractive yet challenging, as flexible matrices struggle to suppress the nonradiative decay of triplet excitons. Herein, self-protective carbonized polymer dots (CPDs) derived from carbonized polyvinyl alcohol (PVA), were embedded in a gel matrix to create flexible URTP materials. Simply tuning the alcoholysis degree of PVA to 88% and reacting it with ethylenediamine under hydrothermal conditions yields NCD₈₈, which exhibits a phosphorescence lifetime of 1.05 s. This benefits from carbonyl/nitrogen clusters that promote intersystem crossing (ISC) and incompletely carbonized PVA chains that restrain nonradiative decay. This strategy effectively resolves the trade-off between ISC efficiency and triplet exciton stabilization, enabling URTP in PVA-based CPDs. Furthermore, by combining freeze–thaw cycling with a solvent exchange strategy, a PVA-based deep eutectic gel was prepared, exhibiting a URTP lifetime of 0.86 s, along with high tensile strength and high toughness. Thus, this study not only resolves the challenge of nonradiative decay in PVA-based self-protective CPDs via simple alcoholysis regulation, but also successfully integrates URTP properties with high mechanical performance into a single gel system, providing a new strategy for developing flexible URTP materials.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70418","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imidazole-Extended Non-Fullerene Acceptors With Tunable Crystallinity for Ultrasensitive, Ultrafast Near-Infrared Organic Photodetectors","authors":"Chengwei Shan,&nbsp;Zihao Li,&nbsp;Han Gao,&nbsp;Yuang Fu,&nbsp;Zhaojin Wang,&nbsp;Xinhui Lu,&nbsp;Xianyong Zhou,&nbsp;Bin-Bin Yu,&nbsp;Philip C. Y. Chow,&nbsp;Aung Ko Ko Kyaw,&nbsp;Chang Liu,&nbsp;Tianshuo Zhao,&nbsp;Hanjian Lai","doi":"10.1002/agt2.70417","DOIUrl":"https://doi.org/10.1002/agt2.70417","url":null,"abstract":"<p>Emerging optoelectronic tasks such as night-vision imaging and wearable health monitoring call for near-infrared (NIR) photodetectors that operate without external bias, exhibit minimal dark current, and respond within nanoseconds. Organic photodetectors (OPDs) have yet to meet these benchmarks, hindered by the scarcity of NIR-absorbing molecules and poor control over active-layer crystallinity. Here we report a unified approach that couples molecular engineering, morphology tuning, and trap-state suppression to accelerate charge dynamics in OPDs. Three non-fullerene acceptors-CA1, CA2, and CA3 were constructed by extending the π-backbone of an imidazole-centered core. DFT calculations and single-crystal analyses show that each additional fused ring systematically modulates backbone planarity and intermolecular packing, which in turn governs charge mobility and defect density. CA2 strikes an optimal balance: its moderate rigidity and favorable surface energy match the donor polymer, producing a bicontinuous fibrillar network with low energetic disorder. Conversely, the highly crystalline CA1 readily self-aggregates, introducing grain boundaries that act as recombination centers. Consequently, the PM6:CA2 blends deliver outstanding OPD performance, with a record-low dark current of 2.54 × 10⁻¹² A cm⁻² at zero bias. They also feature a total noise current of 8.51 × 10⁻¹⁵ A·Hz^−1/2, yielding a detectivity peak of 1.08 × 10¹³ Jones (specific detectivity from real noise) at 840 nm. In addition, the devices exhibit a wide linear dynamic range of 167.8 dB and fast transient responses of 307 ns (rise) and 319 ns (fall). The detector enables cuff-free deep-tissue photoplethysmography and a 10 Mbps optical data link, underscoring the potential of imidazole-extended acceptors for sensitive, ultrafast NIR OPDs.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148617229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroactive Hydrogel Enabling Feedback‑Responsive Monitoring and Regulation of Inflammation for Chronic Wound Therapy","authors":"Hua Wei,&nbsp;Hongyu Zhao,&nbsp;Xiaohan Li,&nbsp;Jingcheng Hao","doi":"10.1002/agt2.70405","DOIUrl":"https://doi.org/10.1002/agt2.70405","url":null,"abstract":"<p>Chronic wounds remain a major clinical challenge due to persistent inflammation, excessive oxidative stress, and impaired tissue regeneration. Herein, we developed a multifunctional hydrogel dressing (GX/ML) by integrating a reactive oxygen species (ROS)-responsive dynamic borate ester cross-linking network with electrocatalytic MXene nanosheets and anti-inflammatory luteolin. The hydrogel enables real-time monitoring of wound inflammation through electrochemical sensing of H₂O₂ levels, while on-demand drug release is triggered by either high ROS or near-infrared irradiation. In vitro and in vivo results demonstrated exceptional ROS-scavenging capability, efficient antibacterial activity (&gt;96% against <i>S. aureus</i> and <i>E. coli</i>), and promotion of macrophage polarization toward the pro-healing M2 phenotype. In a diabetic mouse wound model, the GX/ML hydrogel accelerated wound closure (98.1% healing rate by day 19), enhanced angiogenesis, collagen deposition, and re-epithelialization, and modulated immune responses via cytokine signaling pathways (e.g., NF-κB and NOD-like receptor pathways). Transcriptomic analysis confirmed regulation of genes related to immune inflammation and tissue remodeling. This work provides a synergistic strategy through a smart hydrogel platform that integrates real-time inflammatory monitoring with condition-triggered drug release, offering a feedback-responsive approach for chronic wound management.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148616322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GK PUA Domain Modulates Cables, Cooperativity, and Proline Feedback","authors":"Tianyi Zhang,&nbsp;Qingqing Leng,&nbsp;Siran Li,&nbsp;Huan-Huan Hu,&nbsp;Ji-Long Liu","doi":"10.1002/agt2.70400","DOIUrl":"https://doi.org/10.1002/agt2.70400","url":null,"abstract":"<p>In <i>E. coli</i>, the committed step of proline biosynthesis is initiated by γ-glutamate kinase (GK); however, the regulatory logic governing this enzyme remains poorly understood. <i>E. coli</i> GK (EcGK) possesses an enigmatic C-terminal PUA domain—a motif historically linked to RNA binding but uncharacterized in this context. Here, we demonstrate that EcGK forms metabolic filaments and further assembles into four-stranded metabolic cables. Cryo-EM structures of EcGK filaments reveal two assembly interfaces: one analogous to the eukaryotic P5CS “hook” motif and a novel interface mediated by the PUA domain. Filament formation hinders substrate binding, whereas higher-order cable assembly introduces additional cooperativity in substrate binding. Moreover, we resolve the first proline-bound EcGK structure, revealing the function of the PUA domain and the E135-D148 loop in proline feedback inhibition. Thus, the PUA domain plays dual roles in EcGK regulation: it drives filament/cable assembly to reshape substrate binding kinetics, and also facilitates the E135–D148 loop to enable high sensitivity to proline feedback inhibition. In <i>E. coli</i>, the PUA domain thus modulates proline biosynthesis within physiological ranges of substrates and products.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"7 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70400","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148615991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}