Aggregate (Hoboken, N.J.)最新文献

{"title":"Inside Back Cover: Revealing enhanced dilution effect of conjugated polymers in partially miscible blends","authors":"Hongbo Chen,&nbsp;Ming Hu,&nbsp;Yuehua Zhao,&nbsp;Kaixuan Lyu,&nbsp;Yushuai Xu,&nbsp;Yuansheng Sun,&nbsp;Zhiyuan Xie,&nbsp;Jinying Huang,&nbsp;Dapeng Wang","doi":"10.1002/agt2.738","DOIUrl":"https://doi.org/10.1002/agt2.738","url":null,"abstract":"<p>A counterintuitive optimized dilution effect is unveiled for conjugated polymers in blends. In partially miscible blends, these polymers are more dilute in the majority phase; however, in fully miscible blends, the high miscibility fosters extended chain conformations that undermine dilution. These revelations will inform the design of future generations of polymer light-emitting diodes (e649).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.738","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143117885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back Cover: Droplets Cas13a-RPA measurement delineates potential role for plasma circWDR37 in colorectal cancer","authors":"Jingsong Xu,&nbsp;Li Cao,&nbsp;Shuang Yang,&nbsp;Ying Jian,&nbsp;Yu Liu,&nbsp;Zhen Shen,&nbsp;Qian Liu,&nbsp;Xiang Chen,&nbsp;Min Li,&nbsp;Shun Li,&nbsp;Xiaolei Zuo,&nbsp;Min Li,&nbsp;Hua Wang","doi":"10.1002/agt2.739","DOIUrl":"https://doi.org/10.1002/agt2.739","url":null,"abstract":"<p>This study identified circWDR37 as a promising biomarker for the early diagnosis and prognostic prediction of colorectal cancer (CRC). A detection platform, termed μDCR, was developed utilizing polyethylene glycol 8000 and dextran-10 to enhance fluorescence and improve detection sensitivity. It is anticipated that μDCR will serve as an optimal method for noninvasive CRC screening (e663).\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.739","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143117356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An NIR-II Absorbing Injectable Hydrogel for Boosted Photo-Immunotherapy Toward Human Papillomavirus Associated Cancer","authors":"Xinghua Yu,&nbsp;Lingan Zeng,&nbsp;Xinyue Yang,&nbsp;Zuliang Ren,&nbsp;Xuemei Dong,&nbsp;Ge Meng,&nbsp;Guogang Shan,&nbsp;Dingyuan Yan,&nbsp;Dong Wang,&nbsp;Fei Sun","doi":"10.1002/agt2.743","DOIUrl":"https://doi.org/10.1002/agt2.743","url":null,"abstract":"<p>Human papillomavirus (HPV) is a highly prevalent venereal pathogen accounting for genital warts and various cancers like cervical, anal, and oropharyngeal cancers. Although imiquimod, a topical medication, is commonly used to treat genital warts induced by HPV, its potential as an in situ immune response regulator for HPV-related cancers has rarely been explored. In this study, we developed an innovative synergistic therapeutic platform by integrating near-infrared-II (NIR-II) absorbing aggregation-induced emission (AIE) agent (TPE-BT-BBTD) and imiquimod into an injectable hydrogel named TIH. TPE-BT-BBTD molecule that serves as a photothermal agent, with exposure to a 1064 nm laser, effectively destroys tumor cells and releases tumor-related antigens. During the thermogenesis process, the hydrogel melts and releases imiquimod. The released imiquimod, in conjunction with the dead tumor antigens, stimulates dendritic cell maturation, activating the immune system to ultimately eliminate residual cancer cells. This novel approach combines the immunomodulatory effects of imiquimod with a 1064 nm-excitable photothermal agent in a hydrogel delivery system, offering a promising tactic for combating HPV-associated cancers.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.743","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Rotary Wet-Spinning (RoWS) Biofabrication Directly Affects Proteomic Signature and Myogenic Maturation in Muscle Pericyte–Derived Human Myo-Substitute","authors":"Alessio Reggio,&nbsp;Claudia Fuoco,&nbsp;Francesca De Paolis,&nbsp;Rebecca Deodati,&nbsp;Stefano Testa,&nbsp;Nehar Celikkin,&nbsp;Marina Volpi,&nbsp;Sergio Bernardini,&nbsp;Ersilia Fornetti,&nbsp;Jacopo Baldi,&nbsp;Roberto Biagini,&nbsp;Dror Seliktar,&nbsp;Carmine Cirillo,&nbsp;Wojciech Swieszkowski,&nbsp;Paolo Grumati,&nbsp;Stefano Cannata,&nbsp;Marco Costantini,&nbsp;Cesare Gargioli","doi":"10.1002/agt2.727","DOIUrl":"https://doi.org/10.1002/agt2.727","url":null,"abstract":"<p>Skeletal muscle tissue engineering (SMTE) has recently emerged to address major clinical challenges such as volumetric muscle loss (VML). Here, we report a rotary wet-spinning (RoWS) biofabrication technique for producing human myo-substitutes with biomimetic architectures and functions. Here, we demonstrate how the proposed technique may be used to establish a well-tailored, anisotropic microenvironment that promotes myogenic differentiation of human skeletal muscle–derived pericytes (hPeri). Using high-resolution mass spectrometry–based proteomics with the integration of literature-derived signaling networks, we uncovered that (i) a 3D biomimetic matrix environment (PEG-fibrinogen) confers a less mitogenic microenvironment compared to standard 2D cultures, favoring the formation of contractile-competent bundles of pericyte-derived myotubes in an anchoring-independent 3D state and (ii) the RoWS method promotes an upregulation of muscle matrix structural protein besides increasing contractile machinery proteins with respect to 3D bulk cultures. Finally, in vivo investigations demonstrate that the 3D-biofabricated myo-substitute is fully compatible with the host ablated muscular tissue, exhibiting myo-substitute engraftment and muscle regeneration in a mouse model of VML. Overall, the results show that RoWS offers a superior capability for controlling the myogenic differentiation process on a macroscale and, with future refining, may have the potential to be translated into clinical practice.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.727","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"H2S-Activated Near-Infrared Emission Chemiluminescent Probes for Precise Diagnosis of Inflammations and Tumors","authors":"Mengke Liang,&nbsp;Ling'e Zhang,&nbsp;Bo Yu,&nbsp;Zirui Geng,&nbsp;Huazhen Ge,&nbsp;Ying Sun,&nbsp;Luyu Liu,&nbsp;Xiqun Jiang,&nbsp;Wei Wu","doi":"10.1002/agt2.742","DOIUrl":"https://doi.org/10.1002/agt2.742","url":null,"abstract":"<p>Compared to fluorescence imaging, chemiluminescence imaging does not need external excitation light, and hence presents high imaging depth and signal-to-noise ratio without autofluorescence and phototoxicity, making it a promising tool for biological detection and analysis. However, the target-specific activatable near-infrared emission chemiluminescent probes still need to be developed for the precise diagnosis of diseases. In this paper, we synthesized four direct near-infrared emission Schaap's chemiluminophores (AINCL, AIFCL, ABTCL, and APYCL) by incorporating different electronic acceptors, respectively, and studied the effect of the acceptors on the optical properties of the chemiluminophores. To achieve the specific detection of hydrogen sulfide (H₂S)-related diseases, we used H₂S-cleavable 2,4-dinitrophenylsulfonate to cage the phenol groups in the chemiluminophores. It was demonstrated that the endogenous H₂S in inflammations and tumors could activate effectively the chemiluminescence with high specificity, which provided the precise location of nidus in chemiluminescence imaging and allowed us to perform surgical resection.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.742","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular Gels With Controllable Degradation for Suppressing Tumor Recurrence and Relieving Postoperative Pain","authors":"Jiangtao Lin,&nbsp;Bin Liu,&nbsp;Quan Wang,&nbsp;Yi Duan,&nbsp;Yijie Qiu,&nbsp;Qi Wu,&nbsp;Qi Wang,&nbsp;Yi Dong,&nbsp;Hao Fu,&nbsp;Xinyue Wang,&nbsp;Yourong Duan","doi":"10.1002/agt2.734","DOIUrl":"https://doi.org/10.1002/agt2.734","url":null,"abstract":"<p>Postoperative pain and tumor recurrence are critical challenges following malignant tumor resection. To address these issues, we developed a supramolecular gel delivery system loaded with ropivacaine microcrystals (RopC Gel). Using PEG400 as the solvent, we successfully screened and identified matrix materials capable of forming supramolecular hydrogels through a heating–cooling process. By strategically leveraging the hydrophilic and hydrophobic properties of the gel matrix, we controlled its mechanical strength and degradation rate by adjusting the ratio of hydrophilic to hydrophobic components, resulting in a degradable, injectable, and self-healing gel delivery system. In both rat plantar incision and mouse tumor resection pain models, RopC Gel provided long-lasting analgesia for up to 5 days. Notably, tumor-resected mice treated with RopC Gel demonstrated extended survival and slower tumor progression. Further in vitro and in vivo experiments revealed that RopC Gel affects mitochondrial function by promoting the accumulation of reactive oxygen species in tumor cells, inducing pyroptosis, stimulating immunogenic cell death (ICD), and activating anti-tumor immune responses. This work offers an innovative solution for postoperative tumor resection management. Additionally, the controllable degradation properties outlined in this study provide an efficient strategy for the controlled release of multiple drugs, with the potential for widespread clinical applications.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.734","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PM6/L8-BO Thin Films through Layer-by-Layer Engineering: Formation Mechanism, Energetic Disorder, and Carrier Mobility","authors":"Zihao Wen,&nbsp;Rongkun Zhou,&nbsp;Zilong Zheng,&nbsp;Yi Zhao","doi":"10.1002/agt2.729","DOIUrl":"https://doi.org/10.1002/agt2.729","url":null,"abstract":"<p>Layer-by-layer (LBL) process has emerged as a promising method in the advancement of organic photovoltaics, emphasizing scalability and reproducibility. More importantly, it provides enhanced morphological control for boosting carrier mobility (<i>μ</i>) and power conversion efficiency. By employing a multiscale approach that combined first-principles calculations, molecular dynamics simulations, and kinetic Monte Carlo methods, the relationship between LBL morphology engineering and carrier mobility in donor/acceptor (PM6/L8-BO) thin films is elucidated. During solvent evaporation, the order of solid-phase formation in LBL films was top surface, bottom region, and then the middle region. The early solid precipitation from precursor solutions was acceptor, resulting in a well-ordered molecular arrangement and reducing energy disorder of acceptor LUMO levels. Furthermore, the difference in energy disorders between the A/D blend region and the pure A or D domains enabled LBL morphology engineering to balance electron and hole mobilities, thereby mitigating charge accumulation and recombination. LBL-manufactured films presented higher carrier mobility (<span></span><math>\u0000 <semantics>\u0000 <msubsup>\u0000 <mi>μ</mi>\u0000 <mi>e</mi>\u0000 <mi>LBL</mi>\u0000 </msubsup>\u0000 <annotation>$mu _{mathrm{e}}^{{mathrm{LBL}}}$</annotation>\u0000 </semantics></math> = <span></span><math>\u0000 <semantics>\u0000 <msubsup>\u0000 <mi>μ</mi>\u0000 <mi>h</mi>\u0000 <mi>LBL</mi>\u0000 </msubsup>\u0000 <annotation>$mu _{mathrm{h}}^{{mathrm{LBL}}}$</annotation>\u0000 </semantics></math> = 1.9 × 10⁻³ cm² V⁻¹ s⁻¹) compared to bulk heterojunction (BHJ) films (<span></span><math>\u0000 <semantics>\u0000 <msubsup>\u0000 <mi>μ</mi>\u0000 <mi>e</mi>\u0000 <mi>BHJ</mi>\u0000 </msubsup>\u0000 <annotation>$mu _{mathrm{e}}^{{mathrm{BHJ}}}$</annotation>\u0000 </semantics></math> &gt; <span></span><math>\u0000 <semantics>\u0000 <msubsup>\u0000 <mi>μ</mi>\u0000 <mi>h</mi>\u0000 <mi>BHJ</mi>\u0000 </msubsup>\u0000 <annotation>$mu _{mathrm{h}}^{{mathrm{BHJ}}}$</annotation>\u0000 </semantics></math> = 0.1 × 10⁻³ cm²·V⁻¹ s⁻¹). These mechanisms provided insights into strategies for enhancing charge extraction of photo-generated charge carriers through LBL engineering, driving the development of efficient organic photovoltaic materials.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.729","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Peptide-Derived Aggregation-Induced Emission Nanobioprobe: Unlocking Selective Detection of Fusobacterium nucleatum and Noninvasive Screening of Colorectal Cancer","authors":"Hongyu Liu,&nbsp;Tengling Wu,&nbsp;Yunjian Yu,&nbsp;Youtao Xin,&nbsp;Hegang Lu,&nbsp;Shengke Zhao,&nbsp;Meihui Su,&nbsp;Lu Ga,&nbsp;Alideertu Dong,&nbsp;Mahmoud Elsabahy,&nbsp;Hui Gao","doi":"10.1002/agt2.740","DOIUrl":"https://doi.org/10.1002/agt2.740","url":null,"abstract":"<p>Colorectal cancer (CRC) screening and early diagnosis is an effective strategy for reducing CRC mortality. However, the current detection methods involve exorbitant costs and complex procedures, which are inconvenient for large-scale screening. Given its high prevalence in malignant tissues and feces of CRC patients, <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>) has emerged as a crucial biomarker for the early detection of CRC. Herein, we propose an <i>F. nucleatum</i>-specific recognition strategy for CRC screening and diagnosis. A novel nanobioprobe (AIE-Pep) with aggregation-induced emission (AIE) characteristics was synthesized by conjugating a red/near-infrared (NIR) emissive AIE luminogen (AIEgen) with a FadA-targeting peptide (ASANWTIQYND). The robust binding affinity between the peptide and FadA on <i>F. nucleatum</i> allows AIE-Pep NPs to adhere selectively to <i>F. nucleatum</i>, and emits strong red/NIR fluorescence. In the model of the orthotopic CRC, AIE-Pep NPs can precisely localize <i>F. nucleatum</i> around CRC. Moreover, AIE-Pep NPs demonstrated a limit of detection (LOD) of 82.97 CFU/mL for <i>F. nucleatum</i>, which could significantly differentiate the feces of CRC mice from those of normal mice. Overall, this study presents a pivotal approach to specifically identifying <i>F. nucleatum</i> and holds immense potential for CRC diagnosis.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.740","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revolutionizing Bone Regeneration: Vascularized Bone Tissue Engineering with Advanced 3D Printing Technology","authors":"Jiaxuan Fan,&nbsp;Zichuan Ding,&nbsp;Yongrui Cai,&nbsp;Yahao Lai,&nbsp;Chao Huang,&nbsp;Boyi Jiang,&nbsp;Zongke Zhou,&nbsp;Zeyu Luo","doi":"10.1002/agt2.731","DOIUrl":"https://doi.org/10.1002/agt2.731","url":null,"abstract":"<p>The repair and functional reconstruction of bone defects resulting from trauma, surgical resection, degenerative diseases, and congenital malformations are major clinical challenges. Bone tissue engineering has significant advantages in the treatment of severe bone defects. Vascularized bone repair scaffolds are gradually attracting attention and development because of their excellent biomimetic properties and efficient repair efficiency. Three-dimensional (3D) printing technology, which can be used to fabricate structures at different scales using a wide range of materials, has been used in the production of vascularized bone repair scaffolds. This review discusses the research progress in 3D printing for vascularized bone repair scaffolds. Angiogenesis-osteogenesis coupling in the bone regeneration process is first introduced, followed by a summary of the 3D printing technologies, printing inks, and bioactive factors used to fabricate vascularized bone repair scaffolds. Notably, this review focuses on structural design strategies for vascularized bone repair scaffolds. Finally, the application of vascularized bone repair scaffolds in medicine, as well as challenges and outlooks for future development, are described.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infinite Coordination Polymer Polydopamine Nanocomposites Dual-Pathway Multistep Induction of Long-Term Hyperimmunity Combined With Photothermal-Chemo Synergistic Therapy Colorectal Cancer","authors":"Xiaojiang Zhang,&nbsp;Yujie Zhang,&nbsp;Pengqian Wang,&nbsp;Feiyu Shi,&nbsp;Siyuan Du,&nbsp;Zhe Zhang,&nbsp;Daocheng Wu,&nbsp;Junjun She,&nbsp;Ya Wang","doi":"10.1002/agt2.730","DOIUrl":"https://doi.org/10.1002/agt2.730","url":null,"abstract":"<p>To improve the long-term therapeutic efficacy of colorectal cancer, we propose a synergistic treatment strategy involving dual-pathway, multistep induction of long-term hyperimmunity combined with photothermal-chemotherapy. To implement this strategy, infinite coordination polymer nanoparticles (SN38-Mn(II)-EGCG ICP NPs) were prepared by coordinating SN38, EGCG, and Mn²⁺. These nanoparticles were then coated with polydopamine (PDA) and grafted with folate-PEG-thiol (FA-PEG-SH) onto their surfaces, producing tumor-targeting folate-modified PDA infinite coordination polymer nanocomposites (ICP@FA-PDA nanocomposites). These nanocomposites exhibit a particle size of 94.9 ± 1.6 nm with a high drug loading capacity (83.3% ± 1.5%), drug release under acidic conditions while maintaining stability in physiological environments. Furthermore, each component within the nanocomposites serves multiple functions. Notably, the incorporation of multiple components triggers a powerful antitumor immune effect and establishes enduring immune memory through a dual-pathway and multistep approach, which is produced with the activation of the cGAS-STING pathway and immunogenic cell death (ICD) by a four-component multistep process. Under a low-dose regimen, this approach induces dual-pathway hyperimmunity effect and generates ultra-long immunological memory, marked by a ninefold increase in CD8⁺ T cell infiltration, a fourfold increase in CD4⁺ T lymphocytes, a fourfold reduction in Treg cells, and a fivefold increase in memory T cells. The remarkable therapy efficacy is achieved by hyperimmunity effect combination of SN38 and EGCG chemotherapy and photothermal therapy. In vivo studies demonstrated that mice treated with ICP@FA-PDA nanocomposites achieved complete eradication of cancer within 21 days, with no recurrence observed within 60 days. These nanocomposites hold significant promise and potential for future clinical translation.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 4","pages":""},"PeriodicalIF":13.9,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.730","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}