Aggregate (Hoboken, N.J.)最新文献_第9页

Biomarker-induced gold aggregates enable activatable near-infrared-II photoacoustic image-guided radiosensitization 生物标记物诱导的金聚集体实现了可激活的近红外-II 光声图像引导的放射增敏作用

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-22 DOI: 10.1002/agt2.652

Qinrui Fu, Chuang Wei, Xiao Yang, Mengzhen Wang, Jibin Song

{"title":"Biomarker-induced gold aggregates enable activatable near-infrared-II photoacoustic image-guided radiosensitization","authors":"Qinrui Fu, Chuang Wei, Xiao Yang, Mengzhen Wang, Jibin Song","doi":"10.1002/agt2.652","DOIUrl":"10.1002/agt2.652","url":null,"abstract":"Current radiotherapy (RT) lacks the ability to accurately discriminate between tumor and healthy tissues, resulting in significant radiation-induced damage for patients. Therefore, there is an urgent need for precise RT techniques that can optimize tumor control while minimizing adverse effects on surrounding healthy tissues. In this study, we developed a nanodrug (AuNR@Peptide) composed of furin-responsive RVRR peptide-conjugated AuNRs, which integrates an activatable probe and a radiosensitizer into a single system for accurate tumor localization, enabling image-guided precision RT. Upon reaching the tumor site after intravenous administration, proteolytic cleavage of RVRR substrates on AuNR@Peptide by biomarker triggers aggregation of gold nanorods (AuNRs) into larger aggregates, leading to activation of near-infrared (NIR)-II photoacoustic (PA) signals to precisely localize the tumor and enhance tumor retention by preventing migration and backflow of AuNRs. This significantly amplifies radiosensitivity efficiency. The peak time point at which the NIR-II PA signal was observed at the tumor site after injection serves as a reference for initiating RT, demonstrating substantial improvement in tumor RT through investigations related to radiosensitization mechanisms. The integration of imaging and therapy in this study offers a promising image-guided therapeutic modality for tumors.","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.652","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Versatile and efficient fabrication of signal “turn-on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self-assembled fluorescent gold nanoclusters-antigen aggregates 基于自组装荧光金纳米簇-抗原聚集体的多功能、高效的信号 "开启 "横向流动分析仪，用于超灵敏肉眼检测小分子

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-21 DOI: 10.1002/agt2.644

Mengjia Chao, Shengmei Tai, Minxin Mao, Wenbo Cao, Chifang Peng, Wei Ma, Yongwei Feng, Zhouping Wang

{"title":"Versatile and efficient fabrication of signal “turn-on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self-assembled fluorescent gold nanoclusters-antigen aggregates","authors":"Mengjia Chao, Shengmei Tai, Minxin Mao, Wenbo Cao, Chifang Peng, Wei Ma, Yongwei Feng, Zhouping Wang","doi":"10.1002/agt2.644","DOIUrl":"10.1002/agt2.644","url":null,"abstract":"Fluorescence signal “turn-on” lateral flow immunoassay (FONLFA) through nanomaterial labeled quenching fluorescent nanomaterial has shown significant potential for the detection of small molecules. However, the fluorescent nanomaterial immobilization on nitrocellulose (NC) membrane commonly requires tedious chemical modification and only a few combinations of fluorescence donor and quencher have been applied in FONLFA. In this work, bright fluorescent metal nanoclusters (Prot-AuNCs) were prepared and self-assembled into Prot-AuNCs/antigen aggregates with three typical small molecule antigens, respectively. The aggregates can be readily immobilized on the surface of the NC membrane, indicating that this strip fabrication strategy has good versatility. Moreover, we evaluated the performances of this FONLFA platform by using carbendazim as a model target and investigated four typical nanomaterials as colorimetric nanoprobes and fluorescence quenchers. We found that all the nanoprobes demonstrated significantly improved naked eye detection sensitivity (vLOD) and limits of detection (LODs) in quantitative analysis. Among them, combing the Fe-polydopamine nanoparticles as quencher with the above aggregates, the FONLFA in signal “turn-on” mode achieved 200-fold improved vLOD (0.05 ng mL−1) compared with conventional colorimetric AuNPs-based lateral flow immunoassay (AuNPs-LFA) (10 ng mL−1). In addition, the LOD in quantitative analysis also was improved by 22-fold and the whole test process was completed within 10 min. With the advantages of efficient fabrication, extraordinary sensitization, and good biocompatibility, our FONLFA platform is expected to have great potential in the rapid detection of various small molecules.","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.644","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inside Back Cover: Aggregation and aging of nanoparticle–protein complexes at interfaces studied by evanescent-light scattering microscopy 封底内页：用渐变光散射显微镜研究纳米粒子-蛋白质复合物在界面上的聚集和老化现象

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-20 DOI: 10.1002/agt2.655

Wei Liu, Yuwei Zhu, Hang Jiang, Lidan Zhou, Yinan Li, Jiahao Wu, Jie Han, Cheng Yang, Jianzhong Jiang, To Ngai

引用次数: 0

Back Cover: Special RCA based sensitive point-of-care detection of HPV mRNA for cervical cancer screening 封底：基于特殊 RCA 的宫颈癌筛查 HPV mRNA 床旁灵敏检测技术

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-20 DOI: 10.1002/agt2.656

Yi Long, Shurui Tao, Dongni Shi, Xingyu Jiang, Tian Yu, Yingxi Long, Libing Song, Guozhen Liu

引用次数: 0

Inside Front Cover: Exploring and leveraging aggregation effects on reactive oxygen species generation in photodynamic therapy 封面内页：探索和利用光动力疗法中活性氧生成的聚集效应

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-20 DOI: 10.1002/agt2.654

Zeyan Zhuang, Jianqing Li, Pingchuan Shen, Zujin Zhao, Ben Zhong Tang

引用次数: 0

Front Cover: Rhein-based Pickering emulsion for hepatocellular carcinoma: Shaping the metabolic signaling and immunoactivation in transarterial chemoembolization 封面：治疗肝细胞癌的莱茵基皮克林乳剂：塑造经动脉化疗栓塞术中的代谢信号和免疫激活作用

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-20 DOI: 10.1002/agt2.653

Xiaoliu Liang, Hui Liu, Hu Chen, Xuqi Peng, Zhenjie Li, Minglei Teng, Yisheng Peng, Jiwei Li, Linyu Ding, Jingsong Mao, Chengchao Chu, Hongwei Cheng, Gang Liu

引用次数: 0

Boosting disassembly of π–π stacked supramolecular nanodrugs under tumor microenvironment by introducing stimuli-responsive drug-mates 通过引入刺激响应性药物配体促进π-π叠层超分子纳米药物在肿瘤微环境下的分解

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-20 DOI: 10.1002/agt2.648

Wenzhe Xu, Ruixu Yang, Yingke Xue, Yang Chen, Shuwei Liu, Songling Zhang, Yonggang Wang, Yi Liu, Hao Zhang

{"title":"Boosting disassembly of π–π stacked supramolecular nanodrugs under tumor microenvironment by introducing stimuli-responsive drug-mates","authors":"Wenzhe Xu, Ruixu Yang, Yingke Xue, Yang Chen, Shuwei Liu, Songling Zhang, Yonggang Wang, Yi Liu, Hao Zhang","doi":"10.1002/agt2.648","DOIUrl":"10.1002/agt2.648","url":null,"abstract":"Numerous reports have demonstrated the construction of supramolecular nanodrugs (SNDs) via the π–π stacking of drug molecules for antitumor applications because most drugs possess aromatic rings or other planar conjugate units. However, the destruction of π–π stacking and the subsequent disassembly of SNDs under tumor microenvironment (TME), which is the precondition for drug release, have not been clearly described. In this work, based on a disassembly model of π–π stacked naphthoquinone SNDs, the influence of co-assembled drugs on disassembly is delineated. Both the experimental observation and computational simulation indicate that the disassembly of SNDs under simulated TME highly depends on the disassembly activation energy (ΔEdis) of neighboring π–π stacked molecules. Owing to the high ΔEdis, the disassembly of self-assembled naphthoquinone SNDs is greatly restricted. Meaningfully, the ΔEdis is the sum of a series of activation energy according to the specific stimuli of TME. Thus, a concept of stimuli-responsive drug-mates is proposed for boosting the disassembly of π–π stacked SNDs, namely the foremost co-assembly of π-conjugated drugs with additional drug molecules that possess relatively weak π–π interaction but high TME responsiveness. Further computational simulation reveals that the introduction of stimuli-responsive drug-mates significantly lowers the ΔEdis, thus accelerating the disassembly of SNDs and the release of drug payloads. Holding the advantages of π-conjugated drug library, the concept of stimuli-responsive drug-mates gives an extensive design of π–π stacked SNDs toward heterogeneous nidus microenvironment responsiveness, which highlights the superiority of widely used drug co-assembly strategy in constructing multifunctional SNDs.","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.648","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted regulation of chiroptical properties and self-assembly behaviors of chiral fluorescent polymers 多方面调控手性荧光聚合物的气光特性和自组装行为

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-19 DOI: 10.1002/agt2.642

Youling He, Junqian Zhang, Chaoyang Ma, Junkai Liu, Jingjing Guo, Ting Han, Rongrong Hu, Bing Shi Li, Ben Zhong Tang

{"title":"Multifaceted regulation of chiroptical properties and self-assembly behaviors of chiral fluorescent polymers","authors":"Youling He, Junqian Zhang, Chaoyang Ma, Junkai Liu, Jingjing Guo, Ting Han, Rongrong Hu, Bing Shi Li, Ben Zhong Tang","doi":"10.1002/agt2.642","DOIUrl":"10.1002/agt2.642","url":null,"abstract":"The multifaceted regulation of the chiroptical properties and self-assembly behaviors of chiral fluorescent polymers is of great significance yet remains challenging to achieve. Herein, a series of novel salen-based chiral fluorescent polymers with aggregation-induced emission and varied substitution manners were facilely and efficiently synthesized. Multiple factors were systematically investigated on the chiroptical properties and self-assembly performance of these polymers, which include molecular structures, solvent environments, metal coordination, and liquid crystal (LC) assemblies. Sutle change in the solvent composition can lead to diverse assembly morphologies of all these chiral polymers, from single-handed helical fibers, helical toroids or loops, to spherical structures, consequently leading to an aggregation-reduced circular dichroism (CD) phenomenon. The polymers bearing salen units show highly selective and reversible coordination with Zn2+ and can also induce multiple responses in the absorption, luminescence, CD, and circularly polarized luminescence (CPL) of these chiral fluorescent polymers via a coordination- and dissociation-initiated self-assembly tuning. Furthermore, a small amount of the chiral fluorescent polymer in can induce achiral nematic 4-cyano-4′-n-pentylbiphenyl (5CB) to form ordered chiral nematic LC phase with significant improvement in their CD and CPL signal. The absolute absorption and luminescent dissymmetry factors of the resulting supramolecular assemblies can reach the order of 10−1.","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"5 6","pages":""},"PeriodicalIF":13.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.642","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid degradation of DHX36 revealing its transcriptional role by interacting with G-quadruplex DHX36 的快速降解揭示了其通过与 G 型四核苷酸相互作用而发挥的转录作用

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-19 DOI: 10.1002/agt2.647

Ziang Lu, Jinglei Xu, Yuqi Chen, Yuanyuan Zhou, Xiaolu Zhou, Qi Wang, Qi Wei, Shaoqing Han, Ruiqi Zhao, Xiaocheng Weng, Xiaolian Zhang, Xiang Zhou

{"title":"Rapid degradation of DHX36 revealing its transcriptional role by interacting with G-quadruplex","authors":"Ziang Lu, Jinglei Xu, Yuqi Chen, Yuanyuan Zhou, Xiaolu Zhou, Qi Wang, Qi Wei, Shaoqing Han, Ruiqi Zhao, Xiaocheng Weng, Xiaolian Zhang, Xiang Zhou","doi":"10.1002/agt2.647","DOIUrl":"10.1002/agt2.647","url":null,"abstract":"Accumulating evidence indicates that G-quadruplexes (G4s) are involved in transcriptional regulation. Previous studies have demonstrated that DHX36 preferentially resolves G4s, suggesting its potential impact on gene transcription mediated by these structures. However, systematic validation is required to establish a link between DHX36 activity and its roles in transcriptional regulation. In this study, we investigate the role of DHX36 in transcription. First, we employ the cleavage under targets and tagmentation (CUT&Tag), an efficient method for mapping protein–DNA interactions, to identify the binding sites in the chromatin of MCF-7 cells. Subsequently, we use the auxin-inducible degron (AID) protein degradation system and improved nascent RNA sequencing method acrylonitrile-mediated uridine-to-cytidine conversion sequencing (AMUC-seq) to pinpoint genes directly regulated by DHX36. Our results reveal a significant enrichment of G4 structures at DHX36 target sites, predominantly located in active genomic regions. In vitro assays further demonstrate DHX36's interaction with G4 sequences from three specific oncogenes. These findings underscore the potential role of DHX36 in modulating gene transcription through G4 structures.","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.647","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing enhanced dilution effect of conjugated polymers in partially miscible blends 揭示部分混溶混合物中共轭聚合物的增强稀释效应

IF 13.9

Aggregate (Hoboken, N.J.) Pub Date : 2024-08-19 DOI: 10.1002/agt2.649

Hongbo Chen, Ming Hu, Yuehua Zhao, Kaixuan Lyu, Yushuai Xu, Yuansheng Sun, Zhiyuan Xie, Jinying Huang, Dapeng Wang

{"title":"Revealing enhanced dilution effect of conjugated polymers in partially miscible blends","authors":"Hongbo Chen, Ming Hu, Yuehua Zhao, Kaixuan Lyu, Yushuai Xu, Yuansheng Sun, Zhiyuan Xie, Jinying Huang, Dapeng Wang","doi":"10.1002/agt2.649","DOIUrl":"10.1002/agt2.649","url":null,"abstract":"Recent experiments have shown that hole traps could be suppressed in polymer light-emitting diodes under current stress by diluting the light-emitting conjugated polymers within an “inert” large-bandgap host material. However, it is unclear why there is an enhanced dilution effect in partially miscible blends rather than fully miscible blends, as intuition would suggest that better miscibility leads to better dilution. In this work, we propose a cascade analysis by combining multiple fluorescence microscopic techniques and all-atom molecular dynamics simulations to study the solid-to-solid dilution of poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV) in MEH-PPV/polystyrene (PS) blends and MEH-PPV/poly(vinylcarbazole) (PVK) blends. By varying the molecular weights of PS and PVK, we can regulate their miscibility with MEH-PPV. The results corroborate that the dilution effect is enhanced in partially miscible blends rather than fully miscible ones. This is because, in partially miscible blends undergoing phase separation, the concentration of MEH-PPV is notably decreased in the phase occupying the majority of the volume, leading to an overall greater dilution effect than in fully miscible blends. Moreover, MEH-PPV could adopt the more extended conformation in the fully miscible blend, causing a shorter intermolecular distance to further undermine the dilution effect. These findings explain the seemingly counterintuitive more effective dilution effect observed in the recently reported partially miscible blends and provide guidance for further enhancing the performance of future generations of polymer light-emitting diodes.","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 1","pages":""},"PeriodicalIF":13.9,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142215573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0