Aggregate (Hoboken, N.J.)最新文献

{"title":"A Multifunctional Probe for Visualization of the Nanoscale Distribution of Cholesterol in Cells by Expansion Microscopy","authors":"Gang Wen,&nbsp;Dominic A. Helmerich,&nbsp;Lisa Behringer-Pließ,&nbsp;Markus Sauer","doi":"10.1002/agt2.70104","DOIUrl":"https://doi.org/10.1002/agt2.70104","url":null,"abstract":"<p>Unraveling the nanoscale distribution of small molecules in cells is of central importance for the understanding of cellular functions and the development of drugs. However, particularly the visualization of lipids such as cholesterol—a central compound of cell membranes—with high spatial resolution remains challenging because they cannot be efficiently immobilized for super-resolution microscopy investigations. Here we developed an azido- and amino-modified cholesterol probe that can be efficiently fixed and labeled with fluorophores by click chemistry. In combination with expansion microscopy, its cellular localization and interaction with other cellular proteins can be precisely determined in fixed cells at varying time points after addition. Our approach allows us to detect the endocytic pathway of cholesterol with unprecedented spatial resolution and shows that cholesterol is efficiently ingested in endocytic vesicles and accumulates as cholesterol aggregates with an average size of ∼37 nm in late endosomes and lysosomes, respectively.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the Fe(III) Directed 15-Step Domino Inter- and Intramolecular Progressive Coordinative Oligomerization of a Heterocycle Aggregate","authors":"Kai-Bin Chen,&nbsp;Ting-Ting Wang,&nbsp;Zhi-Wei Xu,&nbsp;Ning Tian,&nbsp;Jin Cai,&nbsp;Wen-Yu Qiu,&nbsp;Bin Zhang,&nbsp;Zheng Yin,&nbsp;Bin Liu,&nbsp;Ming-Hua Zeng","doi":"10.1002/agt2.70094","DOIUrl":"https://doi.org/10.1002/agt2.70094","url":null,"abstract":"<p>Benzo[d]thiazol-2-ylmethanol undergoes progressive oligomerization under solvothermal conditions in the presence of FeCl₃·6H₂O, yielding a heterocyclic aggregate, namely 1,2,3-tris(benzo[<i>d</i>]thiazol-2-yl)-2,9-dihydrobenzo[<i>b</i>]cyclopenta[<i>e</i>][1,4]thiazine. Single-crystal X-ray diffraction analysis was conducted on four distinct compounds isolated during the reaction, and electrospray ionization mass spectrometry (ESI-MS) of both solid products and intermediate reaction solutions enabled the identification of 15 consecutive reaction steps, where Fe(III) was directly involved in eight steps. These transformations comprise nine intermolecular C─C coupling events and six intramolecular ring expansion processes. The heteroatoms (N, O, and S) play distinct mechanistic roles according to their positions within the heterocyclic framework: (1) nitrogen and oxygen coordinate with Fe(III), facilitating activation of the reaction site; (2) homolytic cleavage of the C─O bond promotes C─C coupling reactions; and (3) C─S migration induces intramolecular ring expansion. Notably, theoretical calculations indicate a decrease in Gibbs free energy along the intramolecular reaction pathways, substantiating the proposed mechanism and activation mode, which underscores the essential role of Fe(III) in enabling the reaction progression. Furthermore, an investigation of the photophysical properties revealed that the resulting heterocyclic aggregates exhibit strong luminescence within the 535–610 nm wavelength range, approaching the near-infrared region. These findings highlight the significance of this reaction pathway in the controlled synthesis of functional oligomers and polymers from monomeric precursors, particularly through catalysis by cost-effective metal ions.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70094","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Twinkle Light-Inspired Aggregation-Induced Emission “Lighting-Up” Bacteriophages to Enhance Immunoassays via Spontaneous Amino-Yne Click Reaction","authors":"Xiaoyi Lv,&nbsp;Xirui Chen,&nbsp;Qi Chen,&nbsp;Qing Liu,&nbsp;Mingjian Yao,&nbsp;Weipeng Tong,&nbsp;Hao Fang,&nbsp;Yiping Chen,&nbsp;Yonghua Xiong,&nbsp;Ben Zhong Tang,&nbsp;Xiaolin Huang","doi":"10.1002/agt2.70097","DOIUrl":"https://doi.org/10.1002/agt2.70097","url":null,"abstract":"<p>Traditional fluorescence immunoassays are often hindered by false negatives or quantification inaccuracies, especially at high target concentrations, due to the aggregation-caused quenching effect of fluorescent indicators. This study introduces a novel fluorescence immunoassay strategy that leverages the spontaneous amino-yne click reaction to covalently assemble activated alkyne-based luminogens with aggregation-induced emission characteristics (AIEgens) onto the surface of bifunctional M13 bacteriophages, thereby facilitating efficient “lighting-up” fluorescence signal output in conjunction with magnetic-mediated immunorecognition. To further enhance the load of activated alkyne-based AIEgens and improve the fluorescence “lighting-up” efficiency, M13 bacteriophages were engineered to display varying numbers of surface-exposed lysine residues. This was achieved by inserting different quantities of lysines between the signal peptide and the amino acid sequence of the pVIII protein via a point mutation strategy. Benefiting from the synergy of AIEgen stacking-enhanced fluorescence output and M13 bacteriophage-driven signal amplification, the developed “lighting-up” immunoassay enabled highly sensitive and rapid detection of targets, from small molecules to pathogenic microorganisms. This work provides valuable insights into the design of “lighting-up” AIEgens for enhancing fluorescence immunoassays. Moreover, the proposed strategy offers great versatility, allowing it to be readily adapted to detect other targets simply by pairing the target with the M13 bacteriophages.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 9","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70097","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conjugated Polymer Composite Flexible Wood Hydrogel-Mediated Sequential NIR-II Photothermal and Photodynamic Anti-Bacteria and Macrophage Polarization for Acute Sinusitis","authors":"Lei He,&nbsp;Xiaofeng Ma,&nbsp;Yongze Liu,&nbsp;Genyuan Li,&nbsp;Juqing Cui,&nbsp;Jun Zhang,&nbsp;Yuanyuan Li,&nbsp;Ben Zhong Tang,&nbsp;Wentao Wang","doi":"10.1002/agt2.70096","DOIUrl":"https://doi.org/10.1002/agt2.70096","url":null,"abstract":"<p>Bacterial infection-induced acute sinusitis is prevalent and can easily progress into chronic sinusitis, which is often difficult to treat due to the challenging nature of the site, increased environmental pollution, and bacterial drug resistance prevalent nowadays. To address these challenges, a flexible hydrogel (LM@P/S@CP@Hemin) that involves flexible wood-modified logs, photoactive conjugated polymers, an immunomodulator, and an immobilization hydrogel was prepared for nasal cavity treatment. The flexible wood-modified logs provide mechanical strength support. In vitro, experiments verified that the hydrogel could efficiently induce the photothermal effect under near-infrared-II laser irradiation after deeply penetrating bone and produce reactive oxygen species (ROS) to initiate the photodynamic effect for synergetically eliminating bacteria. The introduction of hemin endows LM@P/S@CP@Hemin hydrogel with a strong immunomodulatory effect on macrophages to achieve anti-inflammation and cellular ROS clearance abilities, which avoids the excessive oxidative stress in the nasal cavity. The results showed that the hydrogel induced an anti-bacterial effect with a 98.5% inhibition rate against methicillin-resistant <i>Staphylococcus aureus</i>, hadexcellent clearance ability of excessive ROS, and promoted anti-inflammatory M2 macrophage generation to relieve inflammation. Meanwhile, transcriptome sequencing and mRNA level measurements revealed that the hydrogel could regulate inflammatory-related genes. In vivo, bacterial infection-induced acute sinusitis rabbit model experiments and histological analysis further confirmed the great therapeutic effect of LM@P/S@CP@Hemin for acute sinusitis based on photothermal and photodynamic therapy. Therefore, LM@P/S@CP@Hemin is an excellent therapeutic material that can adapt to the nasal environment and treat acute sinusitis.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 9","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crown-Ether-Directed Assembly of One-Dimensional Silver(I) Coordination Polymers With Dramatically Enhanced Photoluminescence","authors":"Xiaojiao Yang,&nbsp;Xiao-Lin Ye,&nbsp;Shu-Han Bao,&nbsp;Dong-Nan Yu,&nbsp;Wenya Jiang,&nbsp;Bintao Wu,&nbsp;Shuaiqi Wang,&nbsp;Kangzhou Wang,&nbsp;Tang Yang,&nbsp;Guozong Yue,&nbsp;Samia Kahlal,&nbsp;Jean-Yves Saillard,&nbsp;Jean-François Halet,&nbsp;Jianyu Wei,&nbsp;Kuan-Guan Liu","doi":"10.1002/agt2.70092","DOIUrl":"https://doi.org/10.1002/agt2.70092","url":null,"abstract":"<p>Two novel 18-crown-6-ether (18-C-6) directed one-dimensional silver(I) coordination polymers (1D Ag(I) CPs), formulated as {[(18-C-6)Ag(bpy)]·X}_∞ and {[(18-C-6)Ag(pyz)]·X}_∞ {bpy = 4,4′-bipyridine; pyz = pyrazine; X = BF₄⁻ (<b>Ia, IIa</b>), CF₃SO₃⁻ (<b>Ib, IIb</b>)}, are prepared and structurally determined. The protection of the 18-C-6 macrocycle not only efficiently prevents intermolecular interactions within each 1D Ag(I) CP but also significantly enhances the rigidity of the chain structures, allowing these polymers to exhibit remarkable phosphorescence with intense green-light emission at room temperature. Moreover, <b>IIa</b> and <b>IIb</b> show enhanced photoluminescence quantum yields and aggregation-induced emission properties, which can be attributed to their close-packed structure modes in the crystalline states. Interestingly, upon mixing with commercial resin, <b>IIa</b> can serve as an efficient and stable luminescent ink for 3D printing. The resulting printed structures demonstrate exceptional irradiation stability, retaining their luminescence properties without any quenching over a three-month period. This work not only provides a facile strategy to prepare luminescent 1D Ag(I) CPs but also shows the promise of their utilization in optical devices.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning for Organic Fluorescent Materials","authors":"Jiamin Zhong,&nbsp;Wei Zhu,&nbsp;Shoutao Shen,&nbsp;Nan Zhou,&nbsp;Meiyang Xi,&nbsp;Kui Du,&nbsp;Dong Wang,&nbsp;Ben Zhong Tang","doi":"10.1002/agt2.70089","DOIUrl":"https://doi.org/10.1002/agt2.70089","url":null,"abstract":"<p>Organic fluorescent materials (OFMs), characterized by their unique molecular structures and exceptional optical properties, have demonstrated significant potential in diverse applications such as bioimaging, sensors, and display technologies. Nevertheless, the reliance on chemists' intuition and experience in the traditional design of OFMs, coupled with the high cost and lack of scalability of conventional methods such as fluorescence detection and Density Functional Theory (DFT) calculations, makes it difficult to keep up with the rapid development of the field. The advent of machine learning (ML) has introduced transformative possibilities, enabling data-driven exploration of the intricate relationships between molecular structures and fluorescence properties. Herein, we review the applications of ML in the innovative design of OFMs with an emphasis on the workflow of modeling, optical property prediction, and OFM design. We also discuss the critical role of data curation and feature engineering in enhancing model performance. Our review provides an overview of commonly used models and assesses their efficacy. We critically examine key challenges such as database construction, model interpretability, and generalization ability, trying to provide a comprehensive framework that advances the integration of ML in the research of organic fluorescent materials, thereby facilitating the development of next-generation materials.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 9","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70089","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interfacial Coordination Induced Crystalline Metallacyclic Membrane for High-Performance Enantioseparation","authors":"Run-Hao Li,&nbsp;Yumei Wang,&nbsp;Yi Liu,&nbsp;Yue Sun","doi":"10.1002/agt2.70095","DOIUrl":"https://doi.org/10.1002/agt2.70095","url":null,"abstract":"<p>Membranes offer an attractive route to efficient enantioseparation, especially compared with energy-intensive techniques like chromatography. However, tuning membrane structure and porosity to separate chiral molecules remains challenging. Here, we present a process for producing intrinsically chiral, ordered discrete metallacycycle <b>1</b> membranes on polyacrylonitrile supports through interfacial coordination-driven self-assembly using organic precursor <b>2</b> and metallic precursor <b>3</b>. These chiral membranes, with their orientated architecture, exhibit ultra-high enantioselectivity (up to 100%) and permeation efficiency for racemic 1-phenylethanol, 1-phenylethylamine, and 2-phenylglycinol. Thermodynamic data and molecular simulations revealed the retarded transport mechanism of the membrane, resulting in highly efficient enantioseparation. Notably, when integrated into a circuit-controlled 3D-printed module, the aligned metallacyclic membrane retained its enantioselectivity for high-value pharmaceutical racemic salbutamol. This approach provides a feasible strategy for creating supramolecular metallacyclic channels in chiral membranes, demonstrating the potential for accurate enantioseparations.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70095","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting the Antimicrobial Activity of Quaternary Ammonium Photosensitizers by Janus-Type AIE Luminogens","authors":"Dongyang Fan,&nbsp;Meng Li,&nbsp;Zipeng Shen,&nbsp;Ying Li,&nbsp;Jingjing Guo,&nbsp;Dong Wang,&nbsp;Ting Han,&nbsp;Ben Zhong Tang","doi":"10.1002/agt2.70087","DOIUrl":"https://doi.org/10.1002/agt2.70087","url":null,"abstract":"<p>Cationic compounds with quaternary ammonium structures are one of the most commonly utilized antibacterial materials, which can effectively overcome the emergence of bacterial drug resistance. Systematic investigation on the structure-activity relationship of such cationic compounds is essential for the development of efficient antimicrobials toward different bacterial strains with clear antimicrobial mechanisms. In this study, we rationally designed and synthesized two quaternary ammonium photosensitizers with aggregation-induced emission (AIE) properties. One possesses a unilaterally charged and Janus-type structure with two positively charged moieties at one tail and two hydrophobic alkyl chains on the other side. The other is a bilaterally symmetric molecule bearing quaternary ammonium structures at both ends. The fluorescence staining experiments, bactericidal assays, and bacterial morphology analyses reveal that the Janus-type AIE luminogen show superior photodynamic antimicrobial activities possibly due to its better disruption of the bacterial membranes. Further theoretical study on the molecule-membrane interaction and molecular dynamics gains deeper insights into the intrinsic relationships between molecular structures and antibacterial activities, which provides a feasible design strategy for high-performance antimicrobial agents.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Electrochemical Synthesis and Solvatochromic Emission Behavior of Single Crystals of a Heteronuclear Platinum(II)-Copper(I) Complex","authors":"Yijia Liu,&nbsp;Limin Zhang,&nbsp;Yun Ma,&nbsp;Pengfei She,&nbsp;Wei Tang,&nbsp;Wai-Yeung Wong","doi":"10.1002/agt2.70088","DOIUrl":"https://doi.org/10.1002/agt2.70088","url":null,"abstract":"<p>In this study, we present a novel multicomponent self-assembly approach that offers good potential for crafting heterometallic complexes with exceptional constitutional control. This method generates metal ions from a sacrificial anode by using an electric field, which then coordinates with a metal complex precursor. As a proof-of-concept demonstration, we successfully synthesized single crystals of a heterometallic platinum(II)-copper(I) (Pt[II]-Cu[I]) complex at an exceptionally rapid rate within 30 s by applying a voltage to an acetonitrile solution of [Pt(ppy)(CN)₂]⁻Bu₄N⁺, using copper foil as the anode. Intriguingly, by finely adjusting the intensity and duration of the electric field, we achieved a variety of supramolecular structures, spanning from spherical to rod-like and even flower-like morphologies. Additionally, we found that the photoluminescence property of the resultant crystal can be reversibly shifted among green, orange, and cyan by merely altering the solvent environment. Finally, the crafted heterometallic Pt(II)-Cu(I) complex has shown great promise in advanced anti-counterfeiting applications.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70088","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supramolecular Assembly of Triterpenoids: Current State and Biomedical Perspectives","authors":"Jie Zhong,&nbsp;Juan C. Mareque-Rivas,&nbsp;Xinmiao Lan,&nbsp;Yu-Xiong Su","doi":"10.1002/agt2.70081","DOIUrl":"https://doi.org/10.1002/agt2.70081","url":null,"abstract":"<p>Triterpenoids exhibit remarkable pharmacological characteristics and have garnered significant research attention, owing to their unique backbone structures and numerous modification sites. Recent advancements in supramolecular chemistry have highlighted the potential of triterpenoids to form organized assemblies through noncovalent interactions, affording versatile functional properties. By leveraging their unique structural characteristics and biological activities, innovative strategies can be developed to enhance the efficacy and safety of biomedical therapies. This review describes the recent advances in triterpenoids serving as (i) functional groups for aggregation-induced emission, (ii) building blocks for self-transportation and drug delivery, (iii) potential gelators for rational hydrogel design, and (iv) cholesterol alternatives for optimizing lipid-based nanoparticles. The biomedical perspectives of triterpenoid-based supramolecular assemblies and potential bottlenecks in clinical translation are also discussed, with the hope of offering insights into future research and biomedical applications.</p>","PeriodicalId":72127,"journal":{"name":"Aggregate (Hoboken, N.J.)","volume":"6 8","pages":""},"PeriodicalIF":13.7,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agt2.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144881137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}