Journal of Materials Science: Materials in Medicine最新文献

{"title":"Long-term application of silver nanoparticles in dental restoration materials: potential toxic injury to the CNS","authors":"Kaimei Wang,&nbsp;Shiqi Wang,&nbsp;Jingju Yin,&nbsp;Qiankun Yang,&nbsp;Yi Yu,&nbsp;Lin Chen","doi":"10.1007/s10856-023-06753-z","DOIUrl":"10.1007/s10856-023-06753-z","url":null,"abstract":"<p>Silver nanoparticles (AgNPs) have durable and remarkable antimicrobial effects on pathogenic microorganisms, such as bacteria and fungi, in dental plaques. As such, they are widely added to dental restoration materials, including composite resins, denture bases, adhesives, and implants, to solve the problems of denture stomatitis, peri-implant inflammation, and oral infection caused by the long-term use of these dental restoration materials. However, AgNPs can be absorbed into the blood circulatory system through the nasal/oral mucosa, lungs, gastrointestinal tract, skin, and other pathways and then distributed into the lungs, kidneys, liver, spleen, and testes, thereby causing toxic injury to these tissues and organs. It can even be transported across the blood-brain barrier (BBB) and continuously accumulate in brain tissues, causing injury and dysfunction of neurons and glial cells; consequently, neurotoxicity occurs. Other nanomaterials with antibacterial or remineralization properties are added to dental restoration materials with AgNPs. However, studies have yet to reveal the neurotoxicity caused by dental restoration materials containing AgNPs. In this review, we summarize the application of AgNPs in dental restoration materials, the mechanism of AgNPs in cytotoxicity and toxic injury to the BBB, and the related research on the accumulation of AgNPs to cause changes of neurotoxicity. We also discuss the mechanisms of neurotoxicity caused by AgNPs and the mode and rate of AgNPs released from dental restorative materials added with AgNPs to evaluate the probability of neurotoxic injury to the central nervous system (CNS), and then provide a theoretical basis for developing new composite dental restoration materials.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10587321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49672969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effects of silk and polyethylene terephthalate sutures on postoperative complications in impacted lower third molar surgery","authors":"Orhan Zeynep Dilan,&nbsp;Ciğerim Levent,&nbsp;Kaplan Volkan,&nbsp;Güzel Mehmet,&nbsp;Galayene Abdurrahman,&nbsp;Alsmadi Mohammad,&nbsp;Özyurt Anıl","doi":"10.1007/s10856-023-06756-w","DOIUrl":"10.1007/s10856-023-06756-w","url":null,"abstract":"<div><p>The aim of this study was to compare the efficacy of silk and Polyethylene Terephthalate (PET) sutures on postoperative complications in impacted lower third molar surgery. This prospective, randomized, split-mouth, double-blind clinical study was performed between January 2021 and June 2022 at the Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Van Yüzüncü Yıl University. The patients were categorized into two groups in terms of using suture material. PET suture was used in Group 1, and the silk suture in Group 2 for wound closure following impacted lower third molar surgery. The statistical significance level was accepted as <i>p</i> &lt; 0.05 in the study. Forty patients (21 women, 19 men; mean age: 26.1 ± 7.25 years) were included in the study. When values for swelling and trismus evaluation were analyzed, there was no significant intergroup difference (<i>p</i> &gt; 0.05). However, the VAS values of the silk group patients were higher at the 12th and 24th hours (<i>p</i> &lt; 0.05). In addition, the plaque accumulation value in the silk group was higher than that in the PET group on the second postoperative day (<i>p</i> &lt; 0.05). The results indicated that the plaque accumulation in the PET suture was less on the second postoperative day. Also, PET suture group patients felt less pain during the 12th and 24th hours. These results support to use PET sutures in impacted lower third molar surgery.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 11","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06756-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41229406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mg doping ZIF-8 loaded with Icariin and its antibacterial and osteogenic performances","authors":"Lili Li,&nbsp;Jianghui Zhao,&nbsp;Fengcang Ma,&nbsp;Daihua He,&nbsp;Ping Liu,&nbsp;Wei Li,&nbsp;Ke Zhang,&nbsp;Xiaohong Chen,&nbsp;Lin Song","doi":"10.1007/s10856-023-06755-x","DOIUrl":"10.1007/s10856-023-06755-x","url":null,"abstract":"<div><p>In this study, ICA@Mg-ZIF-8 was synthesized by Mg doping in ZIF-8 and loaded with icariin (ICA). The morphologies and phases were observed and analyzed by SEM, XRD, and the release behaviors of Mg, Zn ions and ICA were tested. Its antibacterial and mineralization performances were evaluated. The results showed that ICA@Mg-ZIF-8 has the same morphology and crystal structure as ZIF-8. ICA@Mg-ZIF-8 showed enhanced antibacterial activity against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, and the antibacterial rate was increased to 87.7 % and 64.0 %, respectively. The results of in vitro mineralization showed that ICA@Mg-ZIF-8 presented better osteogenic performance promoting the uniform deposition of more calcium and phosphorus in simulated body fluids compared to ZIF-8.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 10","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10570214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41187737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycaprolactone/graphene oxide/acellular matrix nanofibrous scaffolds with antioxidant and promyelinating features for the treatment of peripheral demyelinating diseases","authors":"Aishwarya Nagarajan,&nbsp;Nasera Rizwana,&nbsp;Michelle Abraham,&nbsp;Mahima Bhat,&nbsp;Aakanksha Vetekar,&nbsp;Goutam Thakur,&nbsp;Uttara Chakraborty,&nbsp;Vipul Agarwal,&nbsp;Manasa Nune","doi":"10.1007/s10856-023-06750-2","DOIUrl":"10.1007/s10856-023-06750-2","url":null,"abstract":"<div><p>Peripheral demyelinating diseases entail damage to axons and Schwann cells in the peripheral nervous system. Because of poor prognosis and lack of a cure, this group of diseases has a global impact. The primary underlying cause of these diseases involves the inability of Schwann cells to remyelinate the damaged insulating myelin around axons, resulting in neuronal death over time. In the past decade, extensive research has been directed in the direction of Schwann cells focusing on their physiological and neuroprotective effects on the neurons in the peripheral nervous system. One cause of dysregulation in the remyelinating function of Schwann cells has been associated with oxidative stress. Tissue-engineered biodegradable scaffolds that can stimulate remyelination response in Schwann cells have been proposed as a potential treatment strategy for peripheral demyelinating diseases. However, strategies developed to date primarily focussed on either remyelination or oxidative stress in isolation. Here, we have developed a multifunctional nanofibrous scaffold with material and biochemical cues to tackle both remyelination and oxidative stress in one matrix. We developed a nanofibrous scaffold using polycaprolactone (PCL) as a foundation loaded with antioxidant graphene oxide (GO) and coated this bioscaffold with Schwann cell acellular matrix. In vitro studies revealed both antioxidant and remyelination properties of the developed bioscaffold. Based on the results, the developed multifunctional bioscaffold approach can be a promising biomaterial approach for treating demyelinating diseases.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 10","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10556163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41100290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced anti-glioma efficacy of biodegradable periodic mesoporous organosilica nanoparticles through target delivery of chemotherapeutics","authors":"Min Dong,&nbsp;Ying Liu,&nbsp;Biao Liu,&nbsp;Jin Peng,&nbsp;Yuxia Tang,&nbsp;Guangming Lu,&nbsp;Haibin Shi,&nbsp;Feipeng Zhu","doi":"10.1007/s10856-023-06747-x","DOIUrl":"10.1007/s10856-023-06747-x","url":null,"abstract":"<p>Glioma is the most common malignant tumor of the brain and enhancing the efficacy of chemotherapy in glioma is critical for improving patients’ prognosis. In this study, a glioma-targeting drug delivery system is constructed using biodegradable periodic mesoporous organosilica nanoparticles (PMO) that are modified with lactoferrin (Lf) ligands. The obtained PMO is doped with thioether groups and can be degraded in the high concentration of glutathione in tumor cells. The surface area and pore volume of PMO are 772 cm²/g and 0.98 cm³/g, respectively and the loading capacity of doxorubicin (Dox) is as high as 20%. The results of the confocal laser scanning microscope show that the uptake of PMO-Lf@Dox by C6 cells is higher than PMO@Dox. The quantitative analysis of the flow cytometer further demonstrates that more PMO-Lf@Dox enter C6 cells, indicating that the modification of lactoferrin can significantly increase the uptake of C6 cells. Finally, the therapeutic efficacy results show that Lf-modified PMO enhances the inhibitory effect of Dox on C6 cells when incubated for 24 h and 72 h. In summary, this lactoferrin receptor-mediated PMO drug carrier with biodegradability in glutathione in tumor cells can be used to enhance drug delivery into glioma without long-term accumulation in vivo.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 10","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10550876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41091238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-microenvironment responsive nano-carrier system for therapy of prostate cancer","authors":"Lujing Li,&nbsp;Renjie Li,&nbsp;Jiachun Li,&nbsp;Jiyi Yao,&nbsp;Qingyuan Zhang,&nbsp;Qiao Ji,&nbsp;Zuofeng Xu","doi":"10.1007/s10856-023-06749-9","DOIUrl":"10.1007/s10856-023-06749-9","url":null,"abstract":"<div><p>Poor selectivity, low bioavailability and serious systemic side-effects have limited the application of traditional chemotherapy method for treatment of prostate cancer. Stimuli-responsive drug delivery systems for chemotherapy are mainly based on the unique characteristics of tumor microenvironment. In this study, the GSH-sensitive poly-TTG-SS@DTX NPs (DTX-loaded poly-Tetraethylene glycol nanoparticles) were designed and synthesized, which were characterized with nanosized diameter (92.8 ± 2.5 nm) and negatively charged surface charge (−24.7 ± 5.56 mV). Experiments in vitro showed that poly-TTG-SS@DTX NPs had good compatibility to healthy cells and strong anti-tumor effect because of rapid and sustained drug release of DTX from poly-TTG-SS@DTX NPs under the tumor-microenvironment condition. The cellular activity remained greater than 90% when the concentration of poly-TTG-SS NPs reached as high as 100 µg/mL treated on healthy cells. The killing effect of DTX loading NPs group on C4-2 cells was stronger than that of free anti-tumor drug and free DTX combined with the blank nano-carrier (25.21% vs 19.93% vs 20.96%). In conclusion, poly-TTG-SS@DTX NPs may provide a new therapeutic strategy for the chemotherapy of prostate cancer.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 10","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06749-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41080067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased antibiofilm and growth inhibitory effect of Imipenem/Cilastatin nanoliposomes against clinical Pseudomonas aeruginosa isolates","authors":"Faezeh Milani,&nbsp;Khosro Adibkia,&nbsp;Hamed Hamishehkar,&nbsp;Tooba Gholikhani,&nbsp;Farhad Bani,&nbsp;Morteza Milani","doi":"10.1007/s10856-023-06752-0","DOIUrl":"10.1007/s10856-023-06752-0","url":null,"abstract":"<div><p>Numerous infections are linked to <i>Pseudomonas aeruginosa</i>. It is one of the major medical concerns because of virulence and antibiotic resistance. Antibiotic encapsulation in liposomes is a good strategy for controlling infections caused by this microorganism. Evaluation of anti-<i>Pseudomonas aeruginosa</i> effect of liposomal form of Imipenem/Cilastatin in vitro condition. By using the disk agar diffusion technique, the isolates’ pattern of antibiotic resistance was identified. The antibiotic was placed into the nanoliposome after it had been made using the thin layer and ethanol injection techniques. SEM and DLS were used to determine the size, shape, and zeta potential of the encapsulated drug form and the empty nanoliposome. Additionally, Imipenem/Cilastatin encapsulation in nanoliposomes was studied using FT-IR spectroscopy. In the microbial assay experiments the MIC, MBC and MBEC of liposomal and free drug forms were determined. The nanoparticles were spherical, with a diameter ranging from 30 to 39 nm, and the EE% in the thin layer and ethanol injection procedures were 97 and 98, respectively. Imipenem/Cilastatin nanoliposomes showed peaks at 3009 cm⁻¹ and 1650 cm⁻¹, demonstrating the thermodynamic stability for the chemical structure of the drug enclosed and validating the encapsulation of antibiotic in the nanoliposomes. When compared to free drug forms, nanoliposomes had lower MIC and MBC values in the majority of the isolates and had a greater ability to eradicate the biofilm formation. It was shown that the two nanoliposome preparation techniques were more efficient in 80% of the isolates, which had outcomes that were consistent with those of numerous other investigations. Overall, we demonstrated that the antibacterial activity of nanoliposomes was higher than that of the free drug form based on the evaluation of their MIC and MBC. Pharmaceutical nanoliposome techniques provide an excellent future perspective on how to manage microbial infections that are resistant to antibiotics.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 10","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06752-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41080066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"L-serine combined with carboxymethyl chitosan guides amorphous calcium phosphate to remineralize enamel","authors":"Yinghui Wang,&nbsp;Shuting Zhang,&nbsp;Peiwen Liu,&nbsp;Fan Li,&nbsp;Xu Chen,&nbsp;Haorong Wang,&nbsp;Zhangyi Li,&nbsp;Xi Zhang,&nbsp;Xiangyu Zhang,&nbsp;Xu Zhang","doi":"10.1007/s10856-023-06745-z","DOIUrl":"10.1007/s10856-023-06745-z","url":null,"abstract":"<div><p>The aim of this study is to investigate a robust and stable calcium-phosphorus system to remineralize human early enamel caries lesions with nanocomplexes of carboxymethyl chitosan/L-serine/amorphous calcium phosphate (CMC-Ser-ACP) to develop an effective method for mimicking the amelogenin (AMEL) mineralization pattern through ACP assembly. A CMC-Ser-ACP nanocomplex solution was first synthesized by a chemical precipitation method, and then 1% sodium hypochlorite (NaClO) was added to induce ACP phase formation. The morphologies of the nanocomplexes were characterized by transmission electron microscopy (TEM), and zeta potential analysis and Fourier transform infrared spectroscopy (FTIR) were performed to detect surface charge and functional group changes. The subtle changes of the demineralized enamel models induced by the remineralization effect were observed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The CMC-Ser-ACP nanocomplex solution could be preserved without any precipitation for 45 days. After the application of NaClO and through the guidance of Ser, ACP nanoparticles transformed into relatively orderly arranged hydroxyapatite (HAP) crystals, generating an aprismatic enamel-like layer closely integrated with the demineralized enamel, which resulted in enhanced mechanical properties for the treatment of early enamel caries lesions. The CMC-Ser-ACP nanocomplex solution is a remineralization system with great solution stability, and when NaClO is added, it can rapidly regenerate an aprismatic enamel-like layer in situ on the demineralized enamel surface. This novel remineralization system has stable chemical properties and can greatly increase the therapeutic effects against early enamel caries.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10474979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10161450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted delivery of a short antimicrobial peptide (CM11) against Helicobacter pylori gastric infection using concanavalin A-coated chitosan nanoparticles","authors":"Mehrdad Moosazadeh Moghaddam,&nbsp;Shahin Bolouri,&nbsp;Reza Golmohammadi,&nbsp;Mahdi Fasihi-Ramandi,&nbsp;Mohammad Heiat,&nbsp;Reza Mirnejad","doi":"10.1007/s10856-023-06748-w","DOIUrl":"10.1007/s10856-023-06748-w","url":null,"abstract":"<div><p><i>Helicobacter pylori</i> is the cause of most cases of stomach ulcers and also causes some digestive cancers. The emergence and spread of antibiotic-resistant strains of <i>H. pylori</i> is one of the most important challenges in the treatment of its infections. The present study aims to develop a concanavalin A (ConA) coated chitosan (CS) nanocarrier-based drug delivery for the targeted release of peptides to the site of <i>H. pylori</i> infection. Accordingly, chitosan was used as an encapsulating agent for CM11 peptide delivery by applying ionotropic gelation method. Con-A was used for coating CS nanoparticles to target <i>H. pylori</i>. The CS NPs and ConA-CS NPs were characterized by FTIR, dynamic light scattering (DLS), and scanning electron microscopy (SEM). The MIC of CM11-loaded ConA-CS NPs against <i>H. pylori</i> SS1 strain was analyzed in vitro. In order to evaluate the treatment efficiency in vivo, a gastric infection model of <i>H. pylori SS1</i> strain was established in mice and histopathological studies and IL-1β cytokine assay were performed. Based on the results, the size frequency for CS NPs and ConA-CS NPs was about 200 and 350 nm, respectively. The prepared CM11-loaded ConA-CS NPs exhibited antibacterial activity against <i>H. pylori SS1</i> strain with a concentration of 32 µg/ml. The highest healing process was observed in synthesized CM11-loaded ConA-CS NPs treatments and a significant decrease in IL-1β was observed. Our findings highlight the potential of chitosan nanoparticles as a drug delivery vehicle in the treatment of gastric infection model of <i>H. pylori</i> SS1 strain.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><img></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10471652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10144411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced therapeutic effects of mesenchymal stem cell-derived extracellular vesicles within chitosan hydrogel in the treatment of diabetic foot ulcers","authors":"Shuangshuang Yang,&nbsp;Siyu Chen,&nbsp;Chengpeng Zhang,&nbsp;Jing Han,&nbsp;Chunyuan Lin,&nbsp;Xiaohui Zhao,&nbsp;Huizhen Guo,&nbsp;Yi Tan","doi":"10.1007/s10856-023-06746-y","DOIUrl":"10.1007/s10856-023-06746-y","url":null,"abstract":"<div><p>Extracellular vesicles (EVs) derived from human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising candidates for cell-free therapy in various diseases, including chronic cutaneous wounds. However, the lack of standardized protocols for EVs’ preparation and identification poses a significant challenge to their clinical application. Thus, the objective was to develop a safe and efficient method for the large-scale production of hUCMSC-derived EVs while establishing a comprehensive identification protocol encompassing morphology, particle size distribution, protein expression, and purity. This study observed that most of the EVs acquired through the protocol exhibited either a cup-shaped or round-shaped structure, with a median diameter of ~73.25?nm. The proportions of EVs positive for CD9, CD63, and CD81 were 37.5%, 38.6%, and 19.8%, respectively. To enhance their therapeutic potential in wound treatment, EVs were incorporated into chitosan hydrogel, forming chitosan hydrogel-EVs (CS-EVs). Furthermore, it was demonstrated that CS-EVs exhibited continuous release of EVs into the surrounding environment and, importantly, that the released EVs were internalized by human umbilical vein endothelial cells (HUVECs), resulting in significant enhancement of cell migration and angiogenesis. Additionally, in a rat model of diabetic foot ulcers, CS-EVs demonstrated a robust therapeutic effect in promoting wound healing. Following a 15-day treatment period, the group treated with CS-EVs demonstrated an impressive 93.3% wound closure ability, accompanied by a high degree of re-epithelialization. In contrast, the control group exhibited only a 71.5% reduction in wound size. In summary, this study offers solutions for the purification, characterization, and application of EVs in clinical wound treatment. These results not only offer fresh perspectives on the involvement of hUCMSC-derived EVs in wound healing but also introduce a non-invasive approach for applying EVs that holds practical significance in skin repair.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 9","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06746-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5072659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}