Journal of Materials Science: Materials in Medicine最新文献

{"title":"Cobalt-containing borate bioactive glass fibers for treatment of diabetic wound","authors":"Minhui Zhang,&nbsp;Aihua Yao,&nbsp;Fanrong Ai,&nbsp;Jian Lin,&nbsp;Qingge Fu,&nbsp;Deping Wang","doi":"10.1007/s10856-023-06741-3","DOIUrl":"10.1007/s10856-023-06741-3","url":null,"abstract":"<div><p>Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Cobalt is well known for its capacity to induce angiogenesis by stabilizing hypoxia-inducible factor-1α (HIF-1α) and subsequently inducing the production of vascular endothelial growth factor (VEGF). In this study, Co-containing borate bioactive glasses and their derived fibers were fabricated by partially replacing CaO in 1393B3 borate glass with CoO. Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) analyses were performed to characterize the effect of Co incorporation on the glass structure, and the results showed that the substitution promoted the transformation of [BO₃] into [BO₄] units, which endow the glass with higher chemical durability and lower reaction rate with the simulated body fluid (SBF), thereby achieving sustained and controlled Co²⁺ ion release. In vitro biological assays were performed to assess the angiogenic potential of the Co-containing borate glass fibers. It was found that the released Co²⁺ ion significantly enhanced the proliferation, migration and tube formation of the Human Umbilical Vein Endothelial Cells (HUVECs) by upregulating the expression of angiogenesis-related proteins such as HIF-1α and VEGF. Finally. In vivo results demonstrated that the Co-containing fibers accelerated full-thickness skin wound healing in streptozotocin (STZ)-induced diabetic rat model by promoting angiogenesis and re-epithelialization.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06741-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4071937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted drug-loaded PLGA-PCL microspheres for specific and localized treatment of triple negative breast cancer","authors":"Chukwudalu C. Nwazojie,&nbsp;John D. Obayemi,&nbsp;Ali A. Salifu,&nbsp;Sandra M. Borbor-Sawyer,&nbsp;Vanessa O. Uzonwanne,&nbsp;Chinyerem E. Onyekanne,&nbsp;Udom M. Akpan,&nbsp;Killian C. Onwudiwe,&nbsp;Josephine C. Oparah,&nbsp;Olushola S. Odusanya,&nbsp;Winston O. Soboyejo","doi":"10.1007/s10856-023-06738-y","DOIUrl":"10.1007/s10856-023-06738-y","url":null,"abstract":"<div><p>The paper presents the results of the experimental and analytical study of targeted drug-loaded polymer-based microspheres made from blend polymer of polylactic-co-glycolic acid and polycaprolactone (PLGA-PCL) for targeted and localized cancer drug delivery. In vitro sustained release with detailed thermodynamically driven drug release kinetics, over a period of three months using encapsulated targeted drugs (prodigiosin-EphA2 or paclitaxel-EphA2) and control drugs [Prodigiosin (PGS), and paclitaxel (PTX)] were studied. Results from in vitro study showed a sustained and localized drug release that is well-characterized by non-Fickian Korsmeyer–Peppas kinetics model over the range of temperatures of 37 °C (body temperature), 41 °C, and 44 °C (hyperthermic temperatures). The in vitro alamar blue, and flow cytometry assays in the presence of the different drug-loaded polymer formulations resulted to cell death and cytotoxicity that was evidence through cell inhibition and late apoptosis on triple negative breast cancer (TNBC) cells (MDA-MB 231). In vivo studies carried out on groups of 4-week-old athymic nude mice that were induced with subcutaneous TNBC, showed that the localized release of the EphA2-conjugated drugs was effective in complete elimination of residual tumor after local surgical resection. Finally, ex vivo histopathological analysis carried out on the euthanized mice revealed no cytotoxicity and absence of breast cancer metastases in the liver, kidney, and lungs 12 weeks after treatment. The implications of the results are then discussed for the development of encapsulated EphA2-conjugated drugs formulation in the specific targeting, localized, and sustain drug release for the elimination of local recurred TNBC tumors after surgical resection.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06738-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4420095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility analysis and chemical characterization of Mn-doped hydroxyapatite","authors":"L. S. Villaseñor-Cerón,&nbsp;D. Mendoza-Anaya,&nbsp;S. López-Ortiz,&nbsp;R. Rosales-Ibañez,&nbsp;J. J. Rodríguez-Martínez,&nbsp;M. I. Reyes-Valderrama,&nbsp;V. Rodríguez-Lugo","doi":"10.1007/s10856-023-06744-0","DOIUrl":"10.1007/s10856-023-06744-0","url":null,"abstract":"<div><p>The present work studies the effect of Mn doping on the crystalline structure of the Hap synthesized by the hydrothermal method at 200 °C for 24 h, from Ca(OH)₂ and (NH₄)₂HPO₄, incorporating MnCl₂ to 0.1, 0.5, 1.0, 1.5 and 2.0 %wt of Mn concentrations. Samples were characterized by the X-Ray Diffraction technique, which revealed the diffraction peaks that corresponded to the hexagonal and monoclinic phase of the Hap; it was observed that the average size of crystallite decreased from 23.67 to 22.69 nm as the concentration of Mn increased. TEM shows that in all samples, there are two distributions of particle sizes; one corresponds to nanorods with several tens of nanometers in length, and the other in which the diameter and length are very close. FTIR analysis revealed absorption bands corresponding to the PO₄⁻³ and OH⁻ groups characteristic of the Hap. It was possible to establish a substitution mechanism between the Mn and the ions of Ca⁺² of the Hap. From the Alamar blue test, a cell viability of 86.88% ± 5 corresponding to the sample of Hap at 1.5 %wt Mn was obtained, considered non-cytotoxic according to ISO 10993-5. It also evaluated and demonstrated the good osteoinductive properties of the materials, which were verified by histology and immunofluorescence expression of osteogenic markers. Adhesion, viability, biocompatibility and osteoinductive properties, make these materials candidates for future applications in bone tissue engineering with likely uses in regenerative medicine.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06744-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5118806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composite material consisting of microporous beta-TCP ceramic and alginate-dialdehyde-gelatin for controlled dual release of clindamycin and bone morphogenetic protein 2","authors":"Lucas Ritschl,&nbsp;Pia Schilling,&nbsp;Annette Wittmer,&nbsp;Marc Bohner,&nbsp;Anke Bernstein,&nbsp;Hagen Schmal,&nbsp;Michael Seidenstuecker","doi":"10.1007/s10856-023-06743-1","DOIUrl":"10.1007/s10856-023-06743-1","url":null,"abstract":"<div><p>The aim of this study was to produce a composite of microporous β-TCP filled with alginate-gelatin crosslinked hydrogel, clindamycin and bone morphogenetic protein (BMP-2) to prolong the drug-release behaviour for up to 28 days. The most promising alginate-di-aldehyde(ADA)-gelatin gel for drug release from microcapsules was used to fill microporous β-TCP ceramics under directional flow in a special loading chamber. Dual release of clindamycin and BMP-2 was measured on days 1, 2, 3, 6, 9, 14, 21 and 28 by high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA). After release, the microbial efficacy of the clindamycin was checked and the biocompatibility of the composite was tested in cell culture. Clindamycin and the model substance FITC-protein A were released from microcapsules over 28 days. The clindamycin burst release was 43 ± 1%. For the loaded ceramics, a clindamycin release above the minimal inhibitory concentration (MIC) until day 9 and a burst release of 90.56 ± 2.96% were detected. BMP-2 was released from the loaded ceramics in low concentrations over 28 days. The release of active substances from β-TCP and hydrogel have already been extensively studied. Directional flow loading is a special procedure in which the ceramic could act as a stabilizer in the bone and, as a biodegradable system, enables a single-stage surgical procedure. Whether ADA-gelatin gel is suitable for this procedure as a more biodegradable alternative to pure alginate or whether a dual release is possible in this composite has not yet been investigated.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 8","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06743-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5477501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-TCP scaffolds with rationally designed macro-micro hierarchical structure improved angio/osteo-genesis capability for bone regeneration","authors":"Jianlang Feng,&nbsp;Junjie Liu,&nbsp;Yingqu Wang,&nbsp;Jingjing Diao,&nbsp;Yudi Kuang,&nbsp;Naru Zhao","doi":"10.1007/s10856-023-06733-3","DOIUrl":"10.1007/s10856-023-06733-3","url":null,"abstract":"<div><p>The design of hierarchical porous structure in scaffolds is crucial for bone defect regenerative repair. However, bioceramic materials present a challenge in precisely constructing designed micropores owing to the limitation of forming process. To investigate micropore shape influences bone regeneration in bioceramic scaffolds with macropores, hierarchical porous scaffolds with interconnective macropores (~400 μm) and two types of micropores (spherical and fibrous) were prepared using a combination of direct ink writing (DIW) and template sacrifice methods. Compared to the scaffold with spherical micropores, the scaffold with highly interconnected fibrous micropores significantly improved cell adhesion and upregulated osteogenic and angiogenetic-related gene expression in mBMSCs and HUVECs, respectively. Furthermore, in vivo implantation experiments showed that hierarchical scaffolds with fibrous micropores accelerated the bone repair process significantly. This result can be attributed to the high interconnectivity of fibrous micropores, which promotes the transportation of nutrients and waste during bone regeneration. Our work demonstrates that hierarchical porous scaffold design, especially one with a fibrous micropore structure, is a promising strategy for improving the bone regeneration performance of bioceramic scaffolds.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 7","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9893441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host–device interactions: exposure of lung epithelial cells and fibroblasts to nickel, titanium, or nitinol affect proliferation, reactive oxygen species production, and cellular signaling","authors":"Simon D. Pouwels,&nbsp;Alina Sigaeva,&nbsp;Shanna de Boer,&nbsp;Ilse A. Eichhorn,&nbsp;Lisanne Koll,&nbsp;Jeroen Kuipers,&nbsp;Romana Schirhagl,&nbsp;Irene H. Heijink,&nbsp;Janette K. Burgess,&nbsp;Dirk-Jan Slebos","doi":"10.1007/s10856-023-06742-2","DOIUrl":"10.1007/s10856-023-06742-2","url":null,"abstract":"<div><p>Endoscopic implantation of medical devices for the treatment of lung diseases, including airway stents, unidirectional valves and coils, is readily used to treat central airway disease and emphysema. However, granulation and fibrotic tissue formation impairs treatment effectiveness. To date little is known about the interaction between implanted devices, often made from metals, such as nickel, titanium or nitinol, and cells in the airways. Here, we study the response of lung epithelial cells and fibroblasts to implant device materials. The adhesion and proliferation of bronchial epithelial cells and lung fibroblasts upon exposure to 10 × 3 × 1 mm pieces of nickel, titanium or nitinol is examined using light and scanning electron microscopy. Pro-inflammatory cytokine mRNA expression and release, signaling kinase activity and intracellular free radical production are assessed. Nitinol, and to a lesser extent nickel and titanium, surfaces support the attachment and growth of lung epithelial cells. Nitinol induces a rapid and significant alteration of kinase activity. Cells directly exposed to nickel or titanium produce free radicals, but those exposed to nitinol do not. The response of lung epithelial cells and fibroblasts depends on the metal type to which they are exposed. Nitinol induces cellular surface growth and the induction of kinase activity, while exposure of lung epithelial cells to nickel and titanium induces free radical production, but nitinol does not.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 7","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9893442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A tri-layer decellularized, dehydrated human amniotic membrane scaffold supports the cellular functions of human tenocytes in vitro","authors":"Yong Mao,&nbsp;Nikita John,&nbsp;Nicole M. Protzman,&nbsp;Desiree Long,&nbsp;Raja Sivalenka,&nbsp;Shamshad Azimi,&nbsp;Brandon Mirabile,&nbsp;Robert Pouliot,&nbsp;Anna Gosiewska,&nbsp;Robert J. Hariri,&nbsp;Stephen A. Brigido","doi":"10.1007/s10856-023-06740-4","DOIUrl":"10.1007/s10856-023-06740-4","url":null,"abstract":"<div><p>Differences in scaffold design have the potential to influence cell-scaffold interactions. This study sought to determine whether a tri-layer design influences the cellular function of human tenocytes in vitro. The single-layer decellularized, dehydrated human amniotic membrane (DDHAM) and the tri-layer DDHAM (DDHAM-3L) similarly supported tenocyte function as evidenced by improved cell growth and migration, reduced dedifferentiation, and an attenuated inflammatory response. The tri-layer design provides a mechanically more robust scaffold without altering biological activity.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 7","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10366303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9893443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A grooved conduit combined with decellularized tissues for peripheral nerve regeneration","authors":"Enxing Yu,&nbsp;Zhiwu Chen,&nbsp;Yuye Huang,&nbsp;Yibing Wu,&nbsp;Zonghuan Wang,&nbsp;Fangfang Wang,&nbsp;Miaoben Wu,&nbsp;Kailei Xu,&nbsp;Wei Peng","doi":"10.1007/s10856-023-06737-z","DOIUrl":"10.1007/s10856-023-06737-z","url":null,"abstract":"<div><p>Peripheral nerve injury (PNI) is a common and severe clinical disease worldwide, which leads to a poor prognosis because of the complicated treatments and high morbidity. Autologous nerve grafting as the gold standard still cannot meet the needs of clinical nerve transplantation because of its low availability and limited size. The development of artificial nerve conduits was led to a novel direction for PNI treatment, while most of the currently developed artificial nerve conduits was lack biochemical cues to promote nerve regeneration. In this study, we designed a novel composite neural conduit by inserting decellularized the rat sciatic nerve or kidney in a poly (lactic-co-glycolic acid) (PLGA) grooved conduit. The nerve regeneration effect of all samples was analyzed using rat sciatic nerve defect model, where decellularized tissues and grooved PLGA conduit alone were used as controls. The degree of nerve regeneration was evaluated using the motor function, gastrocnemius recovery, and morphological and histological assessments suggested that the combination of a grooved conduit with decellularized tissues significantly promoted nerve regeneration compared with decellularized tissues and PLGA conduit alone. It is worth to note that the grooved conduits containing decellularized nerves have a promotive effect similar to that of autologous nerve grafting, suggesting that it could be an artificial nerve conduit used for clinical practice in the future.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 7","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9893437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective, comparative, clinical study of occlusion rate of peripherally inserted central catheters fabricated of poly(vinyl alcohol)-based hydrogel composite","authors":"Joseph Bunch,&nbsp;Brian Hanley,&nbsp;Daniel Donahue","doi":"10.1007/s10856-023-06736-0","DOIUrl":"10.1007/s10856-023-06736-0","url":null,"abstract":"<div><p>Thrombotic accumulation is associated with surface interactions between blood proteins and vascular access devices. Catheter occlusion results from this process, and is a costly, common, occurrence with peripherally inserted central catheters (PICCs). Hydrophilic catheter materials exhibit antithrombotic properties. This retrospective study evaluates the occurrence of catheter occlusion of PICCs constructed of a poly(vinyl alcohol)-based hydrogel composite known as hydrophilic biomaterial (HBM), compared to thermoplastic polyurethane (TPU) control devices. A total of 121 PICCs, 60 HBM and 61 TPU, were placed in patients with a clinical need and were reviewed for the occurrence of catheter occlusion. The records review found that occlusions occurred in 0/60 (0.0%) of the HBM PICCs and 13/61 (21.3%) of TPU PICCs (<i>p</i> = 0.001). HBM exhibits favorable qualities for vascular access, most importantly its extreme hydrophilicity. Clinically, this may be responsible for the reduction in PICC occlusions, which could improve patient outcomes.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 7","pages":""},"PeriodicalIF":4.2,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10361913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10276069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cissus quadrangularis L extract-loaded tricalcium phosphate reinforced natural polymer composite for guided bone regeneration","authors":"Lele Liao,&nbsp;Weihong Zhu,&nbsp;Cheng Tao,&nbsp;Ding Li,&nbsp;Minzhi Mao","doi":"10.1007/s10856-023-06739-x","DOIUrl":"10.1007/s10856-023-06739-x","url":null,"abstract":"<div><p>Natural medicines plants are significant considerable attention as potential therapeutic agents for bone tissue engineering. <i>Cissus quadrangularis</i> L (CQ). is a potent therapeutic plant known for its own osteogenic properties. In this research work, a phytoconstituents-filled composite was produced by incorporating CQ extract with gelatin (Gel) and pectin (Pec) polymers collective through β- tricalcium phosphate (β-TCP) bioceramic via a green template method. The effect of CQ-filled composite morphology and chemical structural properties, in vitro cytotoxicity, cell proliferation, and differentiation was investigated. FTIR spectroscopic results indicated the prepared materials’ structural confirmation. The CQ extract was the alcoholic -OH merge with the hydroxyl and -NH groups in the range of 3000 cm⁻¹ to 3500 cm⁻¹. Scanning electron microscopy images showed that the β-TCP ceramic was perfectly embedded in Gel-Pec polymeric matrix, which is important for bone regeneration. In vitro cell culture results indicated that β-TCP/Gel-Pec/CQ composite provided 92.0% of a favorable substrate for mesenchymal stem cell viability. The gene expression and RT-PCR studies represent the materials with good osteogenic expression, especially the β-TCP/Gel-Pec/CQ composite is observed at 168.0% and 188.0% for RUNx2 and OCN, respectively. The result of the physicochemical characterizations and cell viability studies suggest that CQ-loaded β-TCP/Gel-Pec composite can serve as a potential biomaterial for bone tissue repair and regeneration.</p><h3>Graphical Abstract</h3>\u0000 <figure><div><div><div><picture><source><img></source></picture></div></div></div></figure>\u0000 </div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"34 7","pages":""},"PeriodicalIF":3.7,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-023-06739-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4756922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}