Titanium-doped phosphate glasses containing zinc and strontium applied in bone regeneration

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Tianyi Tang, Rachel Wandless, Zalike Keskin-Erdogan, Nandin-Erdene Mandakhbayar, Jeong-Hui Park, Hae-Won Kim, Morgana Abramchuk, Felipe P. Daltoe, Jonathan C. Knowles
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Abstract

Phosphate bioactive glass has been studied for its advanced biodegradability and active ion release capability. Our previous research found that phosphate glass containing (P2O5)–(Na2O)–(TiO2)–(CaO)–(SrO) or (ZnO) showed good biocompatibility with MG63 and hMSCs. This study further investigated the application of 5 mol% zinc oxide or 17.5 mol% strontium oxide in titanium-doped phosphate glass for bone tissue engineering. Ti-Ca-Na-Phosphate glasses, incorporating 5% zinc oxide or 17.5% strontium oxide, were made with melting quenching technology. The pre-osteoblast cell line MC3T3-E1 was cultured for indirect contact tests with graded diluted phosphate glass extractions and for direct contact tests by seeding cells on glass disks. The cell viability and cytotoxicity were analysed in vitro over 7 days. In vivo studies utilized the tibial defect model with or without glass implants. The micro-CT analysis was performed after surgery and then at 2, 6, and 12 weeks. Extractions from both zinc and strontium phosphate glasses showed no negative impact on MC3T3-E1 cell viability. Notably, non-diluted Zn-Ti-Ca-Na-phosphate glass extracts significantly increased cell viability by 116.8% (P < 0.01). Furthermore, MC3T3-E1 cells cultured with phosphate glass disks exhibited no increase in LDH release compared with the control group. Micro-CT images revealed that, over 12 weeks, both zinc-doped and strontium-doped phosphate glasses demonstrated good bone incorporation and longevity compared to the no-implant control. Titanium-doped phosphate glasses containing 5 mol% zinc oxide, or 17.5 mol% strontium oxide have promising application potential for bone regeneration research.

Graphical Abstract

Abstract Image

将含锌和锶的掺钛磷酸盐玻璃应用于骨再生。
磷酸盐生物活性玻璃因其先进的生物降解性和活性离子释放能力而备受研究。我们之前的研究发现,含有(P2O5)-(Na2O)-(TiO2)-(CaO)-(SrO)或(ZnO)的磷酸盐玻璃与 MG63 和 hMSCs 具有良好的生物相容性。本研究进一步探讨了在掺钛磷酸盐玻璃中添加 5 mol% 氧化锌或 17.5 mol% 氧化锶在骨组织工程中的应用。采用熔融淬火技术制备了含有 5%氧化锌或 17.5%氧化锶的钛-钙-钠-磷酸盐玻璃。对前成骨细胞系 MC3T3-E1 进行培养,用分级稀释的磷酸盐玻璃提取物进行间接接触试验,并将细胞播种到玻璃片上进行直接接触试验。在体外进行了为期 7 天的细胞存活率和细胞毒性分析。体内研究采用了带或不带玻璃植入物的胫骨缺损模型。在手术后以及 2、6 和 12 周时进行显微 CT 分析。锌和磷酸锶玻璃的提取物对 MC3T3-E1 细胞的存活率没有负面影响。值得注意的是,未稀释的锌-钛-钙-锶-磷酸盐玻璃萃取物显著提高了细胞存活率 116.8%(P
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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