Journal of Materials Science: Materials in Medicine最新文献

{"title":"Preparation and application of modified peach gum polysaccharide in the repair of central nervous system injury.","authors":"An Wang, Zhangheng Huang, Haoqi Chen, Qiqiang Xing, Jing Wang, Peiling Dai, Wangying Dai","doi":"10.1007/s10856-026-07059-6","DOIUrl":"https://doi.org/10.1007/s10856-026-07059-6","url":null,"abstract":"<p><p>Spinal cord injury (SCI) is an irreversible neurological injury that leads to severe motor dysfunction and neurological deficiencies, imposing a large social load. Due to the difficulty of the SCI procedure, the prognosis is normally bad. In recent years, naturally derived hydrogels have garnered increasing attention. Peach gum (PG) primarily consists of macromolecular polysaccharides with abundant hydroxyl and carboxyl groups on its branched chains, making it amenable to modification. This study is the first to prepare carboxymethylated peach gum polysaccharide (CPG) using PG as the raw material and load it with nerve growth factor (NGF) to improve the adverse microenvironment post-SCI and promote axonal regeneration. Carboxymethylation modification of PG was done, and its effective change was verified by infrared spectroscopy and degree of replacement measurements. After loading NGF, its characterization was examined. The mechanical characteristics, thermal behavior, and self-healing ability of CPG + NGF were shown to be outstanding in the results. Cell experiments demonstrated that, compared to the sham-operated group, CPG + NGF significantly promotes neurite outgrowth and development in PC-12 cells. Ultimately, CPG + NGF was implanted into the injured spinal cords of rats, and research demonstrated that CPG + NGF significantly improves the microenvironment of the affected areas, fosters axonal regeneration in severely damaged spinal cord regions, and enhances motor function in the rats. The modified PG biopolymer scaffold developed in this study exhibits superior mechanical strength, excellent cytocompatibility, and enhanced biological functionality. In summary, this research provides new insights into the application of biomacromolecules in SCI repair.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced osteogenesis and antibacterial activity of bioactive glass-coated, epsilon-poly-L-lysine-loaded, polycaprolactone 3D-printed scaffolds with potential use in bone tissue engineering.","authors":"Eddy Shan, Liang Feng, Elena Figuero, Mariano Sanz, Maurizio Tonetti, Jinwu Wang","doi":"10.1007/s10856-026-07065-8","DOIUrl":"https://doi.org/10.1007/s10856-026-07065-8","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the structural properties and biological in vitro performance of 3D-printed polycaprolactone (PCL) scaffolds, coated with bioactive glass (BG) and functionalized with epsilon-poly-L-lysine (EPL) when co-cultured with rat bone marrow mesenchymal stem cells.</p><p><strong>Material and methods: </strong>The physicochemical characterization included scanning electron microscopy, energy dispersive spectroscopy, x-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and degradation kinetics. The biological characterization assessed antibacterial activity using a disk diffusion assay, cell viability of rat bone marrow mesenchymal stem cells with confocal laser scanning microscopy, cell proliferation and cytotoxicity with tetrazolium assay and relative gene and protein expression through reverse transcriptase quantitative polymerase chain reaction and western blot.</p><p><strong>Results: </strong>The physicochemical characterization showed a successful and homogeneous distribution of the ceramic component and confirmed EPL-loading. The PCL-BG-EPL scaffolds demonstrated antibacterial activity against Staphylocccus aureus for up to 7 days. Cell proliferation was significantly higher for PCL-BG and PCL-BG-EPL scaffolds, but cytotoxicity also increased in both groups when comparing day 3 and day 7. The osteogenic potential was enhanced for BG-coated scaffolds, with an upregulation of Alpl, Opn, and Col1a1 genes when compared to controls. However, no significant differences were detected for protein synthesis among the three groups.</p><p><strong>Conclusions: </strong>Composite scaffolds exhibited favorable technical properties and superior biological performance over pure PCL scaffolds.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADA-GEL composite hydrogel films incorporating mesoporous bioactive glass nanoparticles and silver-doped bioactive glass nanoparticles for biomedical applications.","authors":"Asimenia Lekidou, Thomas Papatasos, Qaisar Nawaz, Xanthippi Chatzistavrou, Aldo R Boccaccini","doi":"10.1007/s10856-026-07057-8","DOIUrl":"https://doi.org/10.1007/s10856-026-07057-8","url":null,"abstract":"<p><p>Hydrogels made from natural polymers are widely used in biomedical engineering as they resemble the extracellular matrix. Alginate dialdehyde-gelatin (ADA-GEL) hydrogels are notable for their biocompatibility, biodegradability, and adjustable crosslinking. However, their limited mechanical strength and bioactivity present significant challenges for use in tissue regeneration and wound healing. To overcome these issues, this study explores the addition of 0.1 and 1 wt.% mesoporous bioactive glass nanoparticles (MBGNs) or silver-doped bioactive glass nanoparticles (Ag-BGNs) into ADA-GEL hydrogels to create composite hydrogel films. These nanocomposites were tested in terms of printability, swelling, degradation, antibacterial effects, and in vitro compatibility using MG-63 cells (osteosarcoma cells). MBGNs improved the hydrogels' osteogenic ability and structural stability. At the same time, Ag-BGNs provided vigorous antibacterial activity, especially against Staphylococcus aureus and Escherichia coli, without significantly affecting cell viability and morphology. The use of bioactive and antimicrobial nanoparticles in the ADA-GEL matrix offers a promising approach for developing new soft biomaterials for applications such as bone regeneration, wound healing, and implant coatings.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gelatin methacryloyl/hyaluronic acid hydrogel for localized ropivacaine delivery alleviates neuropathic pain and supports functional recovery after spinal cord injury.","authors":"Xuqi Yu, Liwen Du, Qiong Hu, Dan Xia, Fen Wang, Binnan Hu","doi":"10.1007/s10856-026-07064-9","DOIUrl":"https://doi.org/10.1007/s10856-026-07064-9","url":null,"abstract":"<p><p>Neuropathic pain caused by spinal cord injury severely compromises patients' quality of life. The clinical application of ropivacaine is limited by its short duration of action and the significant side effects associated with repeated administration. In this study, we developed a Gelatin methacryloyl/hyaluronic acid-based hydrogel (Ropi-GelMA/HA) to enable localized and controlled delivery of ropivacaine by photo-crosslinking. In a rat model of spinal cord contusion, Ropi-GelMA/HA was associated with lower Nav1.3 and TNF-α expression and higher NGF and BDNF expression, together with improved motor recovery in rats with SCI. In vitro studies further supported the hydrogel's favorable biocompatibility and controlled release behavior during the early phase after administration. Under the tested dosing regimens, Ropi-GelMA/HA was associated with reduced hepatorenal toxicity and more durable analgesic efficacy compared with free ropivacaine, resulting in prolonged analgesic effects and improved functional outcomes under localized controlled delivery conditions. These findings highlight the potential clinical utility of Ropi-GelMA/HA in the treatment of neuropathic pain following spinal cord injury.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteogenic potential of a novel magnesium-containing calcium silicate-based bioactive glass-ceramic scaffold in critical bone defect.","authors":"George Gonçalves Dos Santos, Carmen Sara Rodrigo-Vázquez, Iorrana Índira Dos Anjos Ribeiro, Izamir Resende Júnior Borges Miguel, Aryon de Almeida Barbosa Júnior, Miguel A Rodríguez, Antonio H de Aza, Fabiana Paim Rosa, Fúlvio Borges Miguel","doi":"10.1007/s10856-026-07051-0","DOIUrl":"https://doi.org/10.1007/s10856-026-07051-0","url":null,"abstract":"<p><p>This study aimed to evaluate the osteogenic potential of a novel magnesium (Mg)-containing calcium silicate (CS)-based glass-ceramic scaffold. For this purpose, twenty-four male Wistar rats were randomly assigned to two experimental groups: Glass-ceramic scaffold developed and sintered at 900 °C/1 h (GCS9) and 1250 °C/8 h (GCS1). The scaffolds were implanted in 8 mm critical bone defects and evaluated at biological points of 15 and 45 days. Histomorphological analysis revealed that the scaffolds completely filled the bone defect, demonstrated biocompatibility, and promoted bone neoformation beyond the defect margins in direct contact with the biomaterial surface. Bone formation was observed throughout the entire defect and within the fragmented scaffolds in its central regions. Both groups exhibited mild chronic granulomatous inflammation in the interstitial tissue formed between the biomaterial fragments. Histomorphometric analysis revealed a greater bone neoformation area in the GCS1 group (44%) at 45 days, with a statistically significant difference between the biological points in this group (ANOVA p = 0.048). Therefore, this study demonstrated that Mg-containing CS-based glass-ceramic scaffolds, particularly GCS1, were notably bioactive and osteoconductive, exhibiting significant osteogenic potential for critical bone defect repair and showing promise for future clinical applications.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147809188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of ceria nanoclusters against delayed paraplegia after spinal cord ischemia.","authors":"Wang Yang, Ling Zou, Qianqian Wu, Lu Yang, Yonghui Wu, Qingshan Liu, Guangyou Shi, Renqing Jiang, Ying Xia, Jian Sun, Xiaochao Yang","doi":"10.1007/s10856-026-07062-x","DOIUrl":"https://doi.org/10.1007/s10856-026-07062-x","url":null,"abstract":"<p><p>Spinal cord ischemia reperfusion injury (SCIRI) induced delayed paraplegia is a complex complication of thoracoabdominal aortic surgery. Reactive oxygen species (ROS) play a critical role in the pathogenesis of delayed paraplegia, suggesting that antioxidants could be a promising therapeutic strategy. Ceria nanomaterials have demonstrated potent antioxidant properties and therapeutic potential in various central nervous system disorders. Herein, the preventive effects of ceria nanoclusters (NCs) against delayed paraplegia were investigated. NCs were synthesized using a facile and green method, incubated with albumin, and exhibited excellent biocompatibility and ROS scavenging activity in vitro, providing neuroprotective effects on neuronal cells. To explore the protective effects against delayed paraplegia in vivo, NCs were intravenously injected into SCIRI rabbits both before ischemia and after reperfusion. Post-reperfusion NCs treatment significantly preserved hindlimb motor function and reduced the incidence of delayed paraplegia. Histopathological and biochemical analyses revealed that NCs protected motor neurons, mitigated oxidative stress, and reduced apoptosis and inflammation in the ischemic spinal cord. These results showed that NCs successfully prevented the development of delayed paraplegia by eliminating excessive ROS.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models","authors":"N. Mahmoudi,&nbsp;N. Eslahi,&nbsp;A. Mehdipour,&nbsp;M. Mohammadi,&nbsp;M. Akbari,&nbsp;A. Samadikuchaksaraei,&nbsp;A. Simchi","doi":"10.1007/s10856-025-06892-5","DOIUrl":"10.1007/s10856-025-06892-5","url":null,"abstract":"","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-025-06892-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled drug release and electroconductive performance of 3D printed scaffolds for neural tissue regeneration.","authors":"Busra Oktay, Fatih Ciftci, Israa F Abdulazez, Ayse Betul Bingol, Alpay Kose, Azime Erarslan, Cem Bulent Ustundag","doi":"10.1007/s10856-026-07043-0","DOIUrl":"https://doi.org/10.1007/s10856-026-07043-0","url":null,"abstract":"<p><p>Nerve cell repair is a complex process influenced by genetic factors, damage severity, and treatment type. Although nerve tissue engineering has advanced, many scaffolds still fail to mimic the natural electrical properties of nerve tissue or deliver drugs effectively. To address these issues, this study presents a multifunctional scaffold designed to support nerve regeneration while reducing inflammation and pain. The scaffold was fabricated using 3D microextrusion printing, allowing precise control over geometry and composition. Polyvinyl alcohol (PVA) and collagen (Col) provided biocompatibility and biodegradability, while reduced graphene oxide (rGO) enhanced electrical conductivity. Amoxicillin (Amox) and ibuprofen (Ibu) were incorporated for antibacterial and anti-inflammatory effects. The scaffold exhibited a conductivity of (5.83 ± 0.65) × 10⁻³ S/m, and sustained drug release, with amoxicillin reaching ~0.6 mg/L and ibuprofen ~0.12 mg/L after 480 min. It showed strong antibacterial activity, with inhibition zones of 28.3 ± 3.32 mm (E. coli) and 18.34 ± 2.83 mm (S. aureus). Mechanically, it withstood ~5.5 MPa of stress and over 150% tensile strain. Cell viability exceeded 120%, indicating excellent biocompatibility. These results suggest the scaffold effectively integrates conductivity, structural strength, and therapeutic delivery to promote nerve regeneration.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term disinfection of 3D-printed denture resin: physical and biological in vitro assessments.","authors":"Amanda C Ferro, Caroline C de Oliveira, Bárbara L Morais, Jonatas S de Oliveira, Rodolfo D Piazza, Rodrigo F C Marques, Carlos Mota, Matthew B Baker, Janaina H Jorge","doi":"10.1007/s10856-026-07049-8","DOIUrl":"https://doi.org/10.1007/s10856-026-07049-8","url":null,"abstract":"<p><p>This study evaluated the effects of prolonged overnight immersion in disinfectant solutions on the physical and biological properties of 3D-printed and heat-polymerized polymethyl methacrylate (PMMA) denture base materials. Four solutions were tested: distilled water (control), 1% sodium hypochlorite, 2% chlorhexidine digluconate, and a disinfectant soap (Lifebuoy®). Daily cycles of 8 h in disinfectant solutions and 16 h in distilled water were performed for up to 6 months to represent overnight disinfection and daily use. The evaluated parameters included color change, water contact angle, Vickers hardness, surface roughness and topography, residual antimicrobial activity against Candida albicans biofilm, and cytotoxicity in L-929 cells. Color change remained within clinically acceptable thresholds for all groups, with Lifebuoy® showing values comparable to the control. Water contact angles decreased after immersion, while surface roughness was stable up to 3 months and decreased at 6 months, particularly in PMMA. Hardness increased in heat-polymerized specimens, whereas 3D-printed materials showed greater stability over time. 3D-printed resins exhibited higher C. albicans biofilm formation than PMMA. Chlorhexidine digluconate resulted in the greatest reduction in fungal growth and metabolic activity, followed by sodium hypochlorite and Lifebuoy®. Most groups showed no cytotoxic effects, except for moderate cytotoxicity of chlorhexidine at 3 months. In conclusion, 3D-printed resin showed superior physical performance, while PMMA demonstrated lower Candida colonization. Chlorhexidine was the most effective antibiofilm agent despite time-dependent cytotoxicity, while Lifebuoy® served as a non-cytotoxic alternative.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed PCL scaffolds: optimising material selection for specific bone regeneration applications.","authors":"Izabella Rajzer, Renata Novotna, Anna Kurowska, Jarosław Janusz, Janusz Fabia, Adam Jabłoński, Wojciech Piekarczyk, Oscar Castano, Magdalena Ziąbka, Jana Frankova","doi":"10.1007/s10856-026-07047-w","DOIUrl":"https://doi.org/10.1007/s10856-026-07047-w","url":null,"abstract":"<p><p>Significant clinical challenges are posed by large bone defects, necessitating the use of scaffolds that combine mechanical stability with osteoinductive properties. While polycaprolactone (PCL) lends itself well to 3D printing, its limited bioactivity means it needs to be modified with bioactive additives. Various additives have been proposed to enhance PCL scaffolds, but a systematic comparative evaluation of their mechanical and biological effects is lacking. This hinders the optimal selection of materials for specific applications. In this study, we compared the effects of four additives-silver nanoparticles (AgNPs), osteogenon (OST), zinc oxide (ZnO) and vitroceramic calcium phosphate (CaPNPs)-when incorporated at a concentration of 0.5 wt% into 3D-printed PCL scaffolds. We comprehensively evaluated the mechanical properties, thermal characteristics, and osteoblast biocompatibility using tensile testing, differential scanning calorimetry, and SaOS-2 cell culture assays (MTT test, activity of alkaline phosphatase, production of collagen I and fluorescent staining with acridine orange or phalloidin). ZnO modification significantly enhanced the mechanical properties (834% strain at break versus 658% for pure PCL and an increased Young's modulus), as well as supporting cell viability (87 and 85%). Meanwhile, CaPNPs demonstrated the highest level of early-stage cell viability (103% after 24 h), although this was not statistically significant. All additives exhibited non-cytotoxic profiles with >80% cell viability and demonstrated time-dependent increases in alkaline phosphatase activity, but further evaluation for clinical application is essential. These findings provide evidence-based guidance for selecting PCL scaffold additives based on specific application requirements: ZnO is optimal for mechanically demanding applications, while CaPNPs could be optimal for facilitating rapid cell integration.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147759269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}