Journal of Materials Science: Materials in Medicine最新文献

{"title":"True-bone-ceramics / type I collagen scaffolds for repairing osteochondral defect","authors":"Yuhan Jiang,&nbsp;Tenghai Li,&nbsp;Yingyue Lou,&nbsp;Bingzhang Liu,&nbsp;Yilin Liu,&nbsp;Tian Li,&nbsp;Duo Zhang","doi":"10.1007/s10856-024-06852-5","DOIUrl":"10.1007/s10856-024-06852-5","url":null,"abstract":"<div><p>In recent years, the incidence of cartilage defects has increased dramatically, and its etiology is complex and varied. Osteochondritis dissecans (OCD), as one of the main etiologies, damages both cartilage and bone tissues and can progress to severe osteoarthritis, which has been one of the difficult problems for clinicians. The vigorous development of material science and tissue engineering provides new ideas for the treatment of OCD, in which the selection of scaffold materials is particularly important. In this study, true-bone-ceramics (TBC), which has good mechanical strength and osteoconductivity, and type I collagen (COL1), which has excellent biocompatibility, were chosen as scaffold materials to co-construct the TBC/COL1 scaffold for osteochondral repair. In order to ensure the most appropriate collagen coating concentration, three experimental groups (1, 5, 12 mg/ml) were set up. Through the physicochemical property test, biocompatibility analysis and in vivo implantation experiments of composite scaffolds, 12 mg/ml TBC/COL1 scaffolds present the best repair effect among the three groups.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"36 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06852-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper-enriched hydroxyapatite coatings obtained by high-velocity suspension flame spraying. Effect of various gas parameters on biocompatibility","authors":"Long-Quan R. V. Le,&nbsp;M. Carolina Lanzino,&nbsp;Matthias Blum,&nbsp;Anika Höppel,&nbsp;Ali Al-Ahmad,&nbsp;Andreas Killinger,&nbsp;Rainer Gadow,&nbsp;Wolfgang Rheinheimer,&nbsp;Michael Seidenstuecker","doi":"10.1007/s10856-024-06846-3","DOIUrl":"10.1007/s10856-024-06846-3","url":null,"abstract":"<div><p>Hydroxyapatite (HAp)-coated bone implants are frequently used for orthopaedic or dental implants since they offer high biocompatibility and osteoconductivity. Yet, problems such as infections, e.g. periprosthetic joint infections, occur when implanting foreign material into the body. In this study, HAp coatings were produced via high-velocity suspension flame spraying (HVSFS). This method allows for the production of thin coatings. We investigated the effects of different gas parameters on the coating properties and on the biocompatibility, which was tested on the human osteosarcoma cell line MG63. Furthermore, Copper (Cu) was added to achieve antibacterial properties which were evaluated against standard microorganisms using the airborne assay. Three gas parameter groups (low, medium, and high) with different Cu additions (0 wt.%, 1 wt.% and 1.5 wt.%) were evaluated. Our findings show that porosity as well as hardness can be controlled through gas parameters. Furthermore, we showed that it is possible to add Cu through external injection. The Cu content in the coating as well as the release varies with different gas parameters. Both antibacterial efficacy as well as biocompatibility are affected by the Cu content. We could significantly reduce the amount of colony-forming units (CFU) in all coatings for <i>E. coli</i>, CFU for <i>S. aureus</i> was reduced by adding 1.5 wt.% of Cu to the coating. The biocompatibility testing showed a cytotoxicity threshold at a Cu-release of 14.3 mg/L in 120 hours. Based on our findings, we suggest medium gas parameters for HVSFS and the addition of 1 wt.% Cu to the coating. With these parameters, a reasonable antibacterial effect can be achieved while maintaining sufficient biocompatibility.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06846-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, and application potential of chitosan/acrylamide composite hydrogels as skin expanders","authors":"Chenxi Zhang,&nbsp;Chenjie Tan,&nbsp;Hangchong Shen,&nbsp;Qianqian Xu,&nbsp;Jiadong Pan,&nbsp;Xin Wang","doi":"10.1007/s10856-024-06812-z","DOIUrl":"10.1007/s10856-024-06812-z","url":null,"abstract":"<div><p>Hydrogels are currently widely used in regenerative medicine and wound repair due to their superior biocompatibility, reliable mechanical strength, and good morphological memory. We aimed to prepare a self-expanding hydrogel that can be used as a skin expander for the repair of large soft skin tissue defects. Self-expanding hydrogels were prepared by chemical cross-linking, which consisted of water-soluble chitosan (CS), acrylamide (AM), methylene bisacrylamide (NMBA), etc. Five groups of in vitro experiments, including (CS-AM) of 0% (pure AM group), 13.9%, 27.8%, 41.7%, and 55.6%, were conducted to determine mechanical properties, swelling properties, cytotoxicity, etc. In the rat model, both a tight skin area (neck) and a loose skin area (back) were selected for expansion with hydrogels. A total of 27.8% of the CS-AM samples expanded stably under the skin of the rats, achieving 370% expansion in the tight zone and 490% expansion in the flaccid zone. Subcutaneous histopathological examination suggested that the inflammation index of the pericolloid tissue was lower in the CS-AM group than in the pure AM group. Our results demonstrate that self-expanding CS-AM hydrogels have great potential for application as skin expanders.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06812-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borosilicate bioactive glasses with added Mg/Sr enhances human adipose-derived stem cells osteogenic commitment and angiogenic properties","authors":"Jenna M. Tainio,&nbsp;Sari Vanhatupa,&nbsp;Susanna Miettinen,&nbsp;Jonathan Massera","doi":"10.1007/s10856-024-06830-x","DOIUrl":"10.1007/s10856-024-06830-x","url":null,"abstract":"<div><p>Bioactive glasses are one of the most promising materials for applications in bone tissue engineering. In this study, the focus was on borosilicate bioactive glasses with composition 47.12 SiO₂ - 6.73 B₂O₃ - 21.77-x-y CaO - 22.65 Na₂O - 1.72 P₂O₅ - x MgO - y SrO (mol%). These compositions are based on silicate S53P4 bioactive glass, from where 12.5% of SiO₂ is replaced with B₂O₃, and additionally, part of CaO is substituted for MgO and/or SrO. The impact of ion release, both as extract and in direct contact, on human adipose-derived stem cells’ (hADSCs) viability, proliferation, ECM maturation, osteogenic commitment and endothelial marker expression was assessed. Osteogenic media supplements were utilized with the extracts, and in part of the direct cell/material culturing conditions. While it has been reported in other studies that boron release can induce cytotoxicity, the glasses in this study supported cells viability and proliferation. Moreover, borosilicate’s, especially with further Mg/Sr substitutions, upregulated several osteogenic markers (such as <i>RUNX2a, OSTERIX, DLX5, OSTEOPONTIN</i>), as well as angiogenic factors (e.g., <i>vWF</i> and <i>PECAM-1</i>). Furthermore, the studied glasses supported collagen-I production even in the absence of osteogenic supplements, when hADSCs were cultured in contact with the glasses, suggesting that while the bioactive glass degradation products are beneficial for osteogenesis, the glasses surface physico-chemical properties play a significant role on hADSCs differentiation. This study brings critical information on the impact of bioactive glass compositional modification to control glass dissolution and the subsequent influence on stem cells proliferation and differentiation. Furthermore, the role of the material surface chemistry on promoting cell differentiation is reported.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06830-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance evaluation of a low-cost Ti-Mo-Fe (TMF8) as a replacement for Ti-6Al-4V for internal fixation implants used in mandibular angular fractures: a finite element analysis study","authors":"Anirudh Venkatraman Krishnan,&nbsp;Nitin Mathusoothanaperumal Sukanya,&nbsp;Tabishur Rahman,&nbsp;Mohamed A. H. Gepreel","doi":"10.1007/s10856-024-06842-7","DOIUrl":"10.1007/s10856-024-06842-7","url":null,"abstract":"<div><p>Stainless steel and titanium-based alloys have been the gold standard when it comes to permanent implants and magnesium-based alloys have been the best option for bioresorbable alloys. Ti-6Al-4V, Ti-64, with its 110 GPa Young’s Modulus is the most commonly employed alloy to manufacture biomedical implants used for treatment of fractures of skeleton. Recently, researchers have developed a new low-cost and toxic Vanadium-free alternative to this alloy, Ti-3Mo-0.5Fe at.%, namely TMF8. This alloy has a 25% lesser Young’s Modulus compared to Ti-6Al-4V and also demonstrated acceptable mechanical properties while possessing better cell proliferation results. The lower Young’s Modulus can aid in lowering stress shielding effects while its cytocompatibility could enhance healing. This work, therefore, tries to use finite element analyses to compare these two alloys (Ti-64 and TMF8) from a practical structural point of view to analyse the advantages and disadvantages of this new alloy and how a low-cost biocompatible alternative (TMF8) can actually prove to be a more viable option. The analyses confirm that TMF8 shows almost similar biomechanics performance to Ti-64 alloy (and in acceptable range) in bone plate fixation of mandibular angular fracture treatment.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06842-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and antibacterial properties of medical stainless steel and titanium modified by alumina and hafnia films prepared by atomic layer deposition","authors":"Ivan Spajić,&nbsp;Miguel Gonçalves Morais,&nbsp;Cláudia Monteiro,&nbsp;M. Cristina L. Martins,&nbsp;Ana Paula Pêgo,&nbsp;Ingrid Milošev","doi":"10.1007/s10856-024-06841-8","DOIUrl":"10.1007/s10856-024-06841-8","url":null,"abstract":"<div><p>New methods for producing surfaces with suitable biocompatible properties are desirable due to increasing demands for biomedical devices. Stainless steel 316 L and cp- titanium specimens were coated with thin films of alumina and hafnia deposited using the atomic layer deposition method at two temperatures, 180 and 260 °C. The morphology of the films was analysed using scanning electron microscopy, and their surface energies were determined based on drop contact angle measurements. Biocompatibility assays performed using mesenchymal stem cells were evaluated by incubating the specimens and then exposing their extracts to the cells or directly seeding cells on the specimen surfaces. No detrimental effect was noticed for any of the specimens. Antibacterial properties were tested by directly incubating the specimens with the bacteria <i>Staphylococcus aureus</i>. Overall, our data show that all prepared films were biocompatible. Alumina films deposited on cp-titanium at 260 °C outperform the other prepared and tested surfaces regarding antiadhesive properties, which could be related to their low surface energy.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06841-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-titanium coating on glass surface to improve platelet-rich fibrin (PRF) quality","authors":"Mustafa Tunalı,&nbsp;Esra Ercan,&nbsp;Suat Pat,&nbsp;Emrah Sarıca,&nbsp;Aysel Güven Bağla,&nbsp;Nilüfer Aytürk,&nbsp;Duygu Sıddıkoğlu,&nbsp;Vildan Bilgin","doi":"10.1007/s10856-024-06838-3","DOIUrl":"10.1007/s10856-024-06838-3","url":null,"abstract":"<div><p>The quality of platelet-rich fibrin (PRF) is contingent on the surface characteristics interfacing with blood. Titanium’s superior platelet activation, surpassing silica, has made Titanium-platelet-rich fibrin (T-PRF) a favored autogenous bone graft material due to its extended degradation time. Pioneering a novel approach, this study aims to achieve an enhanced fibrin structure using glass tubes coated with nano-titanium, marking the surface’s debut in our PRF production endeavors. Employing a rapid thermionic vacuum arc (TVA) process under high vacuum, we conducted comprehensive analyses of the tubes. Comprehensive analyses, including X-ray diffraction (XRD), atomic force microscopy (AFM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS), were conducted on the nano-titanium-coated glass tubes. Three PRF types were formulated: silica-activated leukocyte- and platelet-rich fibrin (L-PRF, control group), machined-surface titanium tubes (T-PRF), and nano-titanium-coated tubes (nanoT-PRF). Analyses unveiled denser fibrin areas in nanoT-PRF than T-PRF, with the least dense areas in L-PRF. Cell distribution paralled between nanoT-PRF and T-PRF groups, while L-PRF cells were embedded in the fibrin border. NanoT-PRF exhibited the densest autogenous fibrin structure, suggesting prolonged in vivo resorption. Additionally, we explore the potential practicality of single-use production for nanoT-PRF tubes, introducing a promising clinical advancement. This study marks a significant stride in innovative biomaterial design, contributing to the progress of regenerative medicine.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06838-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing osteogenesis and mandibular defect repair with magnesium-modified acellular bovine bone matrix","authors":"Peng Wang,&nbsp;Rui Ge,&nbsp;Biao Li,&nbsp;Li Li,&nbsp;Liwei Han,&nbsp;Xiantong Hu,&nbsp;Gang Xu,&nbsp;Kaitao Yu","doi":"10.1007/s10856-024-06835-6","DOIUrl":"10.1007/s10856-024-06835-6","url":null,"abstract":"<div><p>An acellular bovine bone matrix modified to release Magnesium ions (Mg²⁺) (ABBM-Mg) was prepared and evaluated for its potential in osteogenesis and mandibular defect repair. Mg²⁺ was incorporated into ABBM using an ion exchange method. The microstructure and mechanical properties of both ABBM and ABBM-Mg were analyzed using SEM and a biomechanical testing machine. Cytocompatibility, cell adhesion, and osteogenic differentiation were assessed using various methods including CCK-8, Live/Dead staining, SEM, ALP staining, and qPCR analysis in MC3T3-E1 cells. Additionally, a mandibular defect model in rats was established. The bone defect repair outcomes were evaluated using Micro-CT, histological HE staining, and Masson staining. The study showed that mineralization containing magnesium was redeposited on the surface of the three-dimensional porous ABBM, and the ABBM-Mg scaffold promoted cell proliferation and osteogenic differentiation compared to the ABBM scaffold. In the rat mandibular defect model, the ABBM-Mg scaffold demonstrated superior bone repair ability. This study successfully incorporated Mg²⁺ into ABBM without significantly affecting its microstructure and compressive strength. Furthermore, ABBM-Mg showed sustained release of Mg²⁺ which enhanced cell proliferation, adhesion, and osteogenic differentiation in vitro, and promoted mandibular defect healing in rats. This research opens up new possibilities for the clinical application of functionalized acellular bone matrix.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06835-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142518842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear bond strength between dental adhesive systems and an experimental niobium-based implant material","authors":"N. Brümmer,&nbsp;C. Klose,&nbsp;J-T. Schleich,&nbsp;H. J. Maier,&nbsp;M. Eisenburger,&nbsp;M. Stiesch,&nbsp;P.-C. Pott","doi":"10.1007/s10856-024-06834-7","DOIUrl":"10.1007/s10856-024-06834-7","url":null,"abstract":"<div><p>This study aimed to investigate adhesive shear bond strength (SBS) on an ultrafine-grained niobium alloy (UFG-Nb) that is a potential dental implant material. SBS of three adhesive systems combined with three composites to UFG-Nb was compared to corresponding SBS to Ti-6Al-4V and to zirconia. Specimens of the substrates UFG-Nb, Ti-6Al-4V and zirconia with plane surfaces were sandblasted with Al₂O₃, cleaned and dried. Three adhesive systems (Futurabond U, Futurabond M + , Futurabond M + DCA; all VOCO GmbH, Cuxhaven, Germany) were applied each on specimens of each substrate and light cured. One composite (BifixSE, BifixQM, GrandioSO; all VOCO GmbH) was applied and light cured resulting in 27 groups (<i>n</i> = 10) for all substrate-adhesive-composite-combinations. SBS was measured after 24 h of storage. To simulate aging equally prepared specimens underwent 5000 thermocycles before SBS measurement. There was no significant difference in SBS within the non-aged groups. Among the artificially aged groups, GrandioSO-groups showed a greater variance of SBS than the other composites. All significant differences of corresponding UFG-Nb-, Ti-6Al-4V- and zirconia-groups with same adhesive-composite-combination (ACC) were observed between UFG-Nb and zirconia or Ti-6Al-4V and zirconia but never between the two metallic substrates. The similarity between these materials might show in their adhesive bonding behavior. As there were no differences comparing corresponding groups prior to and after artificial aging, it can be concluded that aging does not affect SBS to UFG-Nb, Ti-6Al-4V and zirconia using the tested ACCs. Adhesive bonding of established ACCs to UFG-Nb is possible resulting in SBS comparable to those on Ti-6Al-4V and zirconia surfaces.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06834-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats","authors":"ZhouShan Tao,&nbsp;Min Yang,&nbsp;Cai-Liang Shen","doi":"10.1007/s10856-024-06803-0","DOIUrl":"10.1007/s10856-024-06803-0","url":null,"abstract":"<div><p>More recently, increased studies have revealed that antioxidants can cure osteoporosis by inhibiting oxidative stress. Tauroursodeoxycholic acid (TUDCA) and Selenium (Se) have been confirmed to possess potent anti-oxidative effects and accelerate bone regeneration. In addition, very little is currently known about the effects of a combination with Se and TUDCA on bone defects in osteoporotic states. We, therefore, aimed to assess the protective effect of combination with Se and TUDCA on bone regeneration and investigate the effect and underlying mechanisms. When MC3T3-E1 was cultured in the presence of H₂H₂, Se, TUDCA and Se/TUDCA therapy could increase the matrix mineralization and promote expression of anti-oxidative stress markers in MC3T3-E1, while reducing intracellular reactive oxygen species (ROS) and mitochondrial ROS levels. Meanwhile, silent information regulator type 1 (SIRT1) was upregulated in response to Se, TUDCA and Se/TUDCA exposures in H₂H₂ treated-MC3T3-E1. In the OVX rat model, Se, TUDCA and Se/TUDCA showed a clear positive effect against impaired bone repair in osteoporosis. The results above demonstrate that Se/TUDCA exhibits superior efficacy in both cellular and animal experiments, as compared to Se and TUDCA. In conclusion, combination with Se and TUDCA stimulates bone regeneration and is a promising candidate for promoting bone repair in osteoporosis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>The release of TUDCA and Se during the degradation of Se/TUDCA can improve the local bone repair ability. At the same time, it can also inhibit cell ROS, and ultimately greatly promote local bone repair.</p></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"35 1","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-024-06803-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}