In vitro cytocompatibility of triclosan coated Polyglactin910 sutures

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2024-06-20 DOI:10.1007/s10856-024-06796-w

Hongrui Ji, Zhiruo Zhang, Chao Wang, Xuewen Li, Guiling Zhang, Danqing Liu

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引用次数: 0

Abstract

Bioabsorbable sutures can improve the medical functions of existing non-absorbable sutures, and may produce new medical effects, and are expected to become a new generation of medical degradable materials. In this study, the cytocompatibility of triclosan coated polyglactin910 sutures (CTS-PLGA910) was analyzed and different concentrations of sutures were prepared. The effects of sutures on the cytotoxicity and cell proliferation of HUVEC were studied by CCK-8 assay. The hemolysis, total antioxidant capacity (T-AOC) activity and nitric oxide (NO) content were investigated to improve the blood compatibility of sutures. The results showed that the hemolysis rate of CTS-PLGA910 was less than 5%. After treatment on HUVEC cells for 48 and 72 h, there was no significant change in NO content in CTS-PLGA910 groups compared with the control group, while T-AOC activity and antioxidant capacity were significantly increased in medium and high dose groups. In summary, the blood compatibility and cell compatibility were significantly improved, which provided a basis for the clinical application of sutures in the future.

Graphical Abstract

Abstract Image

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涂有三氯生的 Polyglactin910 缝合线的体外细胞相容性。

生物可吸收缝合线可改善现有不可吸收缝合线的医疗功能，并可能产生新的医疗效果，有望成为新一代医用可降解材料。本研究分析了三氯生包覆聚乳酸910缝合线（CTS-PLGA910）的细胞相容性，并制备了不同浓度的缝合线。通过 CCK-8 试验研究了缝合线对 HUVEC 细胞毒性和细胞增殖的影响。为了提高缝合线的血液相容性，研究了其溶血、总抗氧化能力（T-AOC）活性和一氧化氮（NO）含量。结果表明，CTS-PLGA910 的溶血率低于 5%。对 HUVEC 细胞处理 48 和 72 h 后，CTS-PLGA910 组的一氧化氮含量与对照组相比无明显变化，而中剂量组和高剂量组的 T-AOC 活性和抗氧化能力则明显提高。综上所述，CTS-PLGA910 的血液相容性和细胞相容性都得到了明显改善，为今后缝合线的临床应用提供了依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.