基于海藻酸盐和磷酸钙纳米颗粒的颗粒填充水凝胶作为骨粘合剂

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Benedikt Kruse, Katarina Vasic, Kai O. Böker, Arndt F. Schilling, Wolfgang Lehmann, Matthias Epple
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引用次数: 0

摘要

临床上显然需要骨粘合剂来替代骨合成。然而,由于手术部位环境潮湿,骨表面覆盖着血液和脂质或蛋白质等生物大分子,因此这是一个具有挑战性的问题。我们开发了一种纳米颗粒载荷水凝胶,这种水凝胶基于硅包覆的磷酸钙/羧甲基纤维素纳米颗粒(CaP/CMC/SiO2)冻干粉和海藻酸钠(2 wt%)水溶液,并对其在空气中和水中的粘合能力进行了优化。最终的糊状物在约一分钟内由磷酸钙纳米粒子释放的钙离子交联,其中含有约 20 wt% 的纳米粒子和 80 wt% 的水。水凝胶的机械性能是通过大量流变测试确定的。这种触变性糊状水凝胶可以用注射器注射。潮湿的骨碎片在空气中的粘附强度约为 84 kPa。在完全浸入水中的 3 个月内,水凝胶都能使皮质骨碎片保持良好的连接。除了水之外,这种材料只包含生物相容性和可生物降解的成分(磷酸钙、CMC、海藻酸)。它只将极低剂量的这些材料(主要是磷酸钙纳米颗粒)带入骨骼部位。用分化成成骨细胞的高密度骨髓造血干细胞进行体外细胞培养,证实了骨粘合剂配方具有良好的生物相容性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A particle-filled hydrogel based on alginate and calcium phosphate nanoparticles as bone adhesive

The clinical need for bone adhesives as an alternative to osteosynthesis is evident. However, this is a challenging problem due to the moist environment in surgical sites with bone surfaces covered with blood and biomolecules like lipids or proteins. A nanoparticle-loaded hydrogel that is based on a freeze-dried powder of silica-coated calcium phosphate/carboxymethyl cellulose nanoparticles (CaP/CMC/SiO2) and an aqueous solution of sodium alginate (2 wt%) was developed and optimized with respect to the gluing ability in air and in water. The final paste was crosslinked within about one minute by calcium ions released from the calcium phosphate nanoparticles and contained about 20 wt% nanoparticles and 80 wt% water. The mechanical properties of the hydrogel were determined by extensive rheological tests. The thixotropic pasty hydrogel can be applied with a syringe. The adhesion strength was about 84 kPa between moist bone fragments in air. The hydrogel kept fragments of cortical bone well connected for >3 months during complete submersion in water. Besides water, the material consists only of biocompatible and biodegradable components (calcium phosphate, CMC, alginate). It carries only a very low dose of these materials into the bone site (mainly calcium phosphate nanoparticles). In-vitro cell culture with hMSCs that differentiated to osteoblasts confirmed a good biocompatibility of the bone adhesive formulation.

Graphical Abstract

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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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