基于介孔二氧化硅纳米颗粒的给药系统对牙龈卟啉单胞菌诱导的骨吸收的抑制作用

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Mengya Li, Jian Sun, Dong Zhao, Wen Zhang, Qingan Xu
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引用次数: 0

摘要

控制和减少牙菌斑的形成在预防和治疗牙周病方面起着至关重要的作用,通常利用抗菌药物来提高治疗效果。介孔二氧化硅纳米粒子(MSN)是一种经美国食品及药物管理局批准的无机纳米材料,具有强大的物理和化学特性,如孔径和孔容量可调、表面易于改性以及生物安全性高。许多研究利用 MSN 来调节药物释放和促进靶向给药。本研究旨在合成一种 MSN-四环素(MSN-TC)复合物,并研究其对牙龈卟啉菌(P. gingivalis)诱导的骨吸收的抑制潜力。通过细菌培养实验评估了 MSN-TC 的抗菌功效。通过在大鼠颅骨周围皮下注射牙龈弧菌,建立了牙龈弧菌诱导的骨吸收模型。采用显微计算机断层扫描评估了 MSN 和 MSN-TC 对骨吸收的抑制作用。此外,还在体外研究了 MSN 和 MSN-TC 对破骨细胞分化的影响。MSN 表现出最佳的孔径和颗粒尺寸,可有效装载并逐渐释放 TC。MSN-TC 对牙龈脓疱病具有显著的抑菌活性。与 MSN 或 TC 处理的大鼠相比,MSN-TC 处理的大鼠的颅骨组织破坏明显减少。此外,MSN 和 MSN-TC 对核因子卡巴Β配体受体激活剂介导的破骨细胞分化均有抑制作用。本研究中合成的 MSN-TC 复合物具有双重功效,既能对牙龈脓疱疮产生抗菌作用,又能抑制破骨细胞分化,从而减轻牙龈脓疱疮引起的骨吸收。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibitory impact of a mesoporous silica nanoparticle-based drug delivery system on Porphyromonas gingivalis-induced bone resorption

Controlling and reducing plaque formation plays a pivotal role in preventing and treating periodontal disease, often utilizing antibacterial drugs to enhance therapeutic outcomes. Mesoporous silica nanoparticles (MSN), an FDA-approved inorganic nanomaterial, possess robust physical and chemical properties, such as adjustable pore size and pore capacity, easy surface modification, and high biosafety. Numerous studies have exploited MSN to regulate drug release and facilitate targeted delivery. This study aimed to synthesize an MSN-tetracycline (MSN-TC) complex and investigate its inhibitory potential on Porphyromonas gingivalis (P. gingivalis)-induced bone resorption. The antibacterial efficacy of MSN-TC was evaluated through bacterial culture experiments. A P. gingivalis-induced bone resorption model was constructed by subcutaneously injecting P. gingivalis around the cranial bone of rats. Micro-computed tomography was employed to assess the inhibitory impact of MSN and MSN-TC on bone resorption. Furthermore, the influence of MSN and MSN-TC on osteoclast differentiation was examined in vitro. The MSN exhibited optimal pore size and particle dimensions for effective loading and gradual release of TC. MSN-TC demonstrated significant bacteriostatic activity against P. gingivalis. MSN-TC-treated rats showed significantly reduced cranial bone tissue destruction compared to MSN or TC-treated rats. Additionally, both MSN and MSN-TC exhibited inhibitory effects on the receptor activator of nuclear factor kappa-Β ligand-mediated osteoclast differentiation. The MSN-TC complex synthesized in this study demonstrated dual efficacy by exerting antibacterial effects on P. gingivalis and by resisting osteoclast differentiation, thereby mitigating bone resorption induced by P. gingivalis.

Graphical Abstract

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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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