Tauroursodeoxycholic acid combined with selenium accelerates bone regeneration in ovariectomized rats

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
ZhouShan Tao, Min Yang, Cai-Liang Shen
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引用次数: 0

Abstract

More recently, increased studies have revealed that antioxidants can cure osteoporosis by inhibiting oxidative stress. Tauroursodeoxycholic acid (TUDCA) and Selenium (Se) have been confirmed to possess potent anti-oxidative effects and accelerate bone regeneration. In addition, very little is currently known about the effects of a combination with Se and TUDCA on bone defects in osteoporotic states. We, therefore, aimed to assess the protective effect of combination with Se and TUDCA on bone regeneration and investigate the effect and underlying mechanisms. When MC3T3-E1 was cultured in the presence of H2H2, Se, TUDCA and Se/TUDCA therapy could increase the matrix mineralization and promote expression of anti-oxidative stress markers in MC3T3-E1, while reducing intracellular reactive oxygen species (ROS) and mitochondrial ROS levels. Meanwhile, silent information regulator type 1 (SIRT1) was upregulated in response to Se, TUDCA and Se/TUDCA exposures in H2H2 treated-MC3T3-E1. In the OVX rat model, Se, TUDCA and Se/TUDCA showed a clear positive effect against impaired bone repair in osteoporosis. The results above demonstrate that Se/TUDCA exhibits superior efficacy in both cellular and animal experiments, as compared to Se and TUDCA. In conclusion, combination with Se and TUDCA stimulates bone regeneration and is a promising candidate for promoting bone repair in osteoporosis.

Graphical abstract

The release of TUDCA and Se during the degradation of Se/TUDCA can improve the local bone repair ability. At the same time, it can also inhibit cell ROS, and ultimately greatly promote local bone repair.

牛磺脱氧胆酸与硒结合可加速卵巢切除大鼠的骨再生
最近,越来越多的研究发现,抗氧化剂可以通过抑制氧化应激来治疗骨质疏松症。牛磺脱氧胆酸(TUDCA)和硒(Se)已被证实具有强大的抗氧化作用,并能加速骨再生。此外,目前人们对 Se 和 TUDCA 的组合对骨质疏松状态下骨缺损的影响知之甚少。因此,我们的目的是评估 Se 和 TUDCA 联合使用对骨再生的保护作用,并研究其效果和内在机制。在H2H2存在下培养MC3T3-E1时,Se、TUDCA和Se/TUDCA疗法可增加MC3T3-E1的基质矿化,促进抗氧化应激标志物的表达,同时降低细胞内活性氧(ROS)和线粒体ROS水平。同时,在H2H2处理的MC3T3-E1中,沉默信息调节因子1型(SIRT1)在Se、TUDCA和Se/TUDCA暴露下上调。在 OVX 大鼠模型中,Se、TUDCA 和 Se/TUDCA 对骨质疏松症中受损的骨修复有明显的积极作用。上述结果表明,与 Se 和 TUDCA 相比,Se/TUDCA 在细胞和动物实验中都表现出更优越的疗效。图解摘要 Se/TUDCA 降解过程中释放的 TUDCA 和 Se 能提高局部骨修复能力。同时,它还能抑制细胞的 ROS,最终极大地促进局部骨修复。
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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