Cartilage defect repair in a rat model via a nanocomposite hydrogel loaded with melatonin-loaded gelatin nanofibers and menstrual blood stem cells: an in vitro and in vivo study

IF 4.2 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Libo Yuan, Ling Yao, Xianzhen Ren, Xusheng Chen, Xu Li, Yongqing Xu, Tao Jin
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引用次数: 0

Abstract

Cartilage damage caused by injuries or degenerative diseases remains a major challenge in the field of regenerative medicine. In this study, we developed a composite hydrogel system for the delivery of melatonin and menstrual blood stem cells (MenSCs) to treat a rat model of cartilage defect. The composite delivery system was produced by incorporation of melatonin into the gelatin fibers and dispersing these fibers into calcium alginate hydrogels. Various characterization methods including cell viability assay, microstructure studies, degradation rate measurement, drug release, anti-inflammatory assay, and radical scavenging assay were used to characterize the hydrogel system. MenSCs were encapsulated within the nanocomposite hydrogel and implanted into a rat model of full-thickness cartilage defect. A 1.3 mm diameter drilled in the femoral trochlea and used for the in vivo study. Results showed that the healing potential of nanocomposite hydrogels containing melatonin and MenSCs was significantly higher than polymer-only hydrogels. Our study introduces a novel composite hydrogel system, combining melatonin and MenSCs, demonstrating enhanced cartilage repair efficacy, offering a promising avenue for regenerative medicine.

Graphical Abstract

大鼠模型软骨缺损的修复:载入褪黑素明胶纳米纤维和月经血干细胞的纳米复合水凝胶的体外和体内研究。
受伤或退行性疾病导致的软骨损伤仍是再生医学领域的一大挑战。在这项研究中,我们开发了一种复合水凝胶系统,用于输送褪黑素和月经血干细胞(MenSCs),以治疗大鼠软骨缺损模型。这种复合输送系统是通过将褪黑素加入明胶纤维并将这些纤维分散到海藻酸钙水凝胶中制成的。水凝胶系统的表征采用了多种表征方法,包括细胞活力测定、微观结构研究、降解率测量、药物释放、抗炎测定和自由基清除测定。将门静脉干细胞包裹在纳米复合水凝胶中,然后植入大鼠全厚软骨缺损模型。在股骨蹄骨上钻孔,直径为 1.3 毫米,用于体内研究。结果表明,含有褪黑激素和造血干细胞的纳米复合水凝胶的愈合潜力明显高于纯聚合物水凝胶。我们的研究介绍了一种新型复合水凝胶系统,它结合了褪黑激素和造血干细胞,显示出更强的软骨修复功效,为再生医学提供了一条前景广阔的途径。
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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