Journal of Materials Science: Materials in Medicine最新文献

{"title":"Synergistic effects of denosumab and BMP-2 on bone regeneration in critical-sized femoral defects in ovariectomized rats","authors":"ZhouShan Tao,&nbsp;WenJing Cheng,&nbsp;Lin Wang,&nbsp;Wei Qi","doi":"10.1007/s10856-026-07081-8","DOIUrl":"10.1007/s10856-026-07081-8","url":null,"abstract":"<div><p>Osteoporotic bone defects pose significant clinical challenges due to poor natural repair capacity. Denosumab (DNB) strongly inhibits osteoclast activity, while Bone morphogenetic protein-2 (BMP-2) effectively promotes bone formation; however, it remains unclear whether combining DNB and BMP-2 can enhance bone repair in estrogen deficiency-induced osteoporosis. We investigated the effects of local BMP-2 implantation and systemic DNB treatment on bone regeneration in ovariectomized (OVX) rats. Micro-computed tomography(Micro-CT) and H&amp;E staining showed that DNB and BMP-2 synergistically accelerated healing of osteoporotic bone defects over 12 weeks. Immunofluorescence, immunohistochemistry, and biomechanical analyses revealed that DNB/BMP-2 increased expression of osteopontin(OPN), osteoclastogenesis inhibitory factor(OPG) and Runt-related transcription factor 2 (RUNX2), and decreased tartrate-resistant acid phosphatase (TRAP) and Tumor Necrosis Factor-α (TNF-α) levels compared to the OVX group (all <i>P</i> &lt; 0.05). Moreover, DNB/BMP-2 significantly improved torsional stiffness and maximum compressive force versus either treatment alone (all <i>P</i> &lt; 0.05). The findings demonstrate that systemic DNB combined with local BMP-2 rapidly promotes femoral metaphyseal bone regeneration in osteoporotic rats.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-026-07081-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-hierarchical biofunctional polymeric biomaterial to promote wound closure","authors":"Zacharie Deruyver,&nbsp;Naïma Ahmed Omar,&nbsp;Claire de Lartigue,&nbsp;Noémie Petit,&nbsp;Louise Hosty,&nbsp;Aline Echalard,&nbsp;Chloé Eudier,&nbsp;Shane Browne,&nbsp;Christophe Egles,&nbsp;Anthony C. Duncan","doi":"10.1007/s10856-026-07055-w","DOIUrl":"10.1007/s10856-026-07055-w","url":null,"abstract":"<div><p>Chronic wounds constitute a significant medical, societal, and economic issue due to the significant delay in wound closure, leading to the increased risk of infection, which extends patients’ time spent in the hospital and negatively impacts overall health. Biofunctional biomaterials have emerged as powerful tools to promote tissue regeneration by mimicking extracellular matrix properties and delivering therapeutic agents. Furthermore, wound closure is a complex and multi-stage process that requires coordination of molecular and cellular factors, such as growth factor signaling and matrix remodeling agents. To address this issue, hierarchically structured and compartmentalized multifunctional biomaterials that include hydrogels have emerged as an effective solution to promote wound healing. In the context of chronic wounds, Reactive Oxygen Species (ROS)-responsive hydrogels are interesting models which specifically trigger a response (e.g., gel opening followed by drug release) in the vicinity of high levels of ROS – specific biomarkers of a chronic state. In this study, we developed a hierarchical multi-layer hybrid biomaterial (HMHB) via the sequential combination of melt electrowriting (MEW) and electrospinning techniques. This HMHB will contain two parts: (i) a macro/micro structured polycaprolactone (PCL) support (MSS) printed via MEW onto which (ii) a polyvinyl alcohol (PVA) electrospun (micro)fibrous film (EFF) was deposited, resulting in a ROS-responsive bioactive antibacterial hydrogel meshwork upper layer that allows for further biofunctionalization. The final construct exhibited a hierarchical architecture composed of a mechanically robust PCL framework and a PVA nanotextured layer. This configuration ensured both structural integrity and homogeneous fiber coverage. Incorporation of Nisin (an antibacterial agent) directly into the EFF prior to the electrospinning step conferred effective antimicrobial activity against <i>Staphylococcus epidermidis</i>, without the need for additional chemical modification. These results establish the HMHB as a promising proof-of-concept for modular and multifunctional wound dressing elaboration.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13529919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable soy protein isolate hydrogel for nanoparticles brain release","authors":"Matilde Ciprandi,&nbsp;Veronica Fontanini,&nbsp;Patrizia Sommi,&nbsp;Umberto Anselmi-Tamburini,&nbsp;Silvia Sesana,&nbsp;Francesca Re","doi":"10.1007/s10856-026-07072-9","DOIUrl":"10.1007/s10856-026-07072-9","url":null,"abstract":"<div><p>Implantable biomaterials for local drug release have been investigated to avoid the ned to cross the blood-brain barrier, which is one of the major limitations of current brain tumors therapies. However, their translation is still limited by inadequate tissue compatibility and suboptimal drug/nanoparticles release. In this study, soy protein isolate (SPI) hydrogels were engineered using microbial transglutaminase (MTGase) as a safe, naturally derived crosslinker, and optimized for brain-relevant constraints. Hydrogels were prepared at 10% and 12% (w/v) SPI and crosslinked with 20 or 40 mg MTGase/g SPI. Crosslinking was confirmed by SDS-PAGE, while scanning electron microscopy revealed a highly porous, microstructure with well-defined cavities. Hydrogels showed high water content (87–89%) and controlled swelling behavior over 72 h that is inversely correlated to MTGase concentration. Rheological analysis demonstrated solid-like behavior (G′ &gt; G′′), shear-thinning viscosity suitable for syringe-based injection, and mechanical stability under dynamic stress. Among the tested samples, the 10% (w/v) SPI hydrogel crosslinked with 20 mg MTGase/g SPI exhibited a storage modulus (~260 Pa) closely matching native brain tissue, making it the most suitable candidate for intracranial application. This formulation enabled controlled release of 100–200 nm liposomes and sustained delivery of doxorubicin-loaded liposomes, resulting in a significant reduction in glioblastoma cell viability in vitro. Importantly, the hydrogel showed no cytotoxicity and did not allow cancer cell adhesion and infiltration, confirming its bioinert nature. Overall, MTGase-crosslinked SPI hydrogels emerge as versatile, scalable, and brain-compatible biomaterials for injectable implants and sustained local nanoparticle release.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-026-07072-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148004282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-assisted synthesis of lithium-bismuth co-doped hydroxyapatite with enhanced antimicrobial and biological performance","authors":"Oyku Copur,&nbsp;Zafer Evis,&nbsp;Hasan Gocmez,&nbsp;Mustafa Tuncer,&nbsp;Sait Altun,&nbsp;Katia Barbaro,&nbsp;Anna A. Zhukova,&nbsp;Iulian V. Antoniac,&nbsp;Julietta V. Rau,&nbsp;Aurel G. Mohan","doi":"10.1007/s10856-026-07067-6","DOIUrl":"10.1007/s10856-026-07067-6","url":null,"abstract":"<div><p>Hydroxyapatites (HA) co-doped with Li⁺ and Bi³⁺ ions were synthesized by a microwave-assisted wet precipitation method to improve their structural, biological, and antibacterial performances. Dual doping of Li⁺ and Bi³⁺ ions led to an increase in crystallinity and crystallite size compared to single doping, indicating successful incorporation of dopants into the HA lattice. Co-doping also resulted in minor lattice distortions without altering the phase purity of HA. Biological characterization using adipose-derived mesenchymal stromal cells revealed that Bi incorporation enhanced cell proliferation and osteogenic activity, whereas excessive Li addition caused partial cytotoxicity. Interestingly, Li/Bi co-doping balanced this effect, leading to improved cell viability and differentiation compared to single-doped samples. Moreover, Li and Bi substitutions imparted significant antimicrobial activity against <i>E. coli</i>, <i>S. aureus</i>, <i>P. aeruginosa</i>, <i>E. faecalis</i>, and <i>C. albicans</i>, demonstrating the dual bioactive and antimicrobial nature of the material. The combined influence of Li⁺ and Bi³⁺ ions effectively modified the crystallinity and biological response of HA, suggesting that Li–Bi co-doped HA is a promising candidate for multifunctional coatings and bone tissue engineering applications.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-026-07067-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147979976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered membrane-coated nanoparticles enhance ferroptosis and microtubule inhibition in prostate cancer","authors":"Kunmu Yang,&nbsp;Yingwei Wang,&nbsp;Chuan Guo,&nbsp;Xiaoxiong Zhang,&nbsp;Jian Wu","doi":"10.1007/s10856-026-07074-7","DOIUrl":"10.1007/s10856-026-07074-7","url":null,"abstract":"<div><p>Prostate cancer (PCa), characterized by its high incidence and progression to treatment-resistant stages, remains a major clinical challenge. Herein, we developed a membrane-coated Cabazitaxel (Cab)@TA–Fe³⁺ nanoplatform for enhanced PCa therapy. The nanoparticles demonstrated notable anticancer effects, with cytotoxicity increasing over time and with dose, along with significant inhibition of cell migration and induction of G2/M phase arrest in LNCaP cells. In addition, treatment markedly increased intracellular reactive oxygen species (ROS) levels, depleted glutathione (GSH), downregulated Glutathione Peroxidase 4 (GPX4) expression, and upregulated NADPH Oxidase 1 (NOX1) and Prostaglandin-Endoperoxide Synthase 2 (PTGS2), indicating enhanced ferroptosis. Ferrostatin-1 partially restored cell viability and reduced ROS accumulation, further supporting the involvement of ferroptosis. Mechanistically, the nanoplatform enables pH/GSH-responsive release of Cab and iron ions in the tumor microenvironment, promoting microtubule disruption and redox imbalance. These combined effects enhance tumor cell death. Overall, this study demonstrates that Cab@TA–Fe³⁺ nanoparticles significantly improve anticancer efficacy by integrating microtubule inhibition and ferroptosis induction, providing a promising strategy for prostate cancer treatment.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-026-07074-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147980034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface modification of urinary catheters with antibiofilm nanocoating: curcumin modified silver nanoparticles against Proteus mirabilis","authors":"N. M. C. Nissanka,&nbsp;K. A. A. Dilhari,&nbsp;G. Priyadarshana,&nbsp;J. A. Munasinghe,&nbsp;M. Dilshani,&nbsp;M. M. Weerasekera","doi":"10.1007/s10856-026-07066-7","DOIUrl":"10.1007/s10856-026-07066-7","url":null,"abstract":"<div><p><i>Proteus mirabilis</i> is a Gram-negative, uropathogenic bacterium with high virulence due to its crystalline biofilm-forming ability, urease production, and antibiotic resistance. Nanotechnology has emerged as a promising field to address such challenges, offering innovative solutions to combat bacterial virulence and antibiotic resistance. This study aimed to develop a urinary catheter surface coated with curcumin-modified silver nanoparticles (Curcumin-AgNPs) to reduce crystalline biofilm formation by <i>P.mirabilis</i>. The coating was assessed using Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy(AFM), scanning electron microscopy-energy-dispersive X-ray analysis(SEM-EDX), and contact angle assays. The antimicrobial efficacy was examined by direct contact assay, serial plate transfer test (SPTT), and time kill assay. Coated catheters were experimented with using an in vitro bladder model over 2, 7, and 14 days, and at the point of catheter blockage. Antibiofilm efficacy was examined using tetrazolium salt 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), crystal violet(CV) assays, SEM-EDX, and atomic absorption spectroscopy(AAS) analysis. P-values &lt; 0.05 were considered statistically significant. FTIR, AFM, SEM, and EDX analyses confirmed the successful coating of the Foley catheter with curcumin-AgNPs. A contact angle measurement of 120.11° demonstrated the hydrophobic nature of the coated catheter. The direct contact assay revealed inhibition zones for coated catheters (11.85 ± 1.86 mm). The coating demonstrated strong antibiofilm activity, with 70 - 80% biomass reduction and 60-80% metabolic inhibition even at the point of catheter blockage. AAS, SEM-EDX analysis showed reduced Ca²⁺ and Mg²⁺ deposition in coated catheters. The findings indicate that curcumin-AgNPs coatings markedly improve the antibacterial and antibiofilm efficacy of urinary catheters, successfully inhibiting crystalline biofilm formation.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13529892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol-loaded chitosan nanoparticles and adipose-derived stem cell–conditioned medium for liver protection against ischemia–reperfusion injury","authors":"Longyan Chen,&nbsp;Jianhua Shi,&nbsp;Saman Jalili","doi":"10.1007/s10856-026-07036-z","DOIUrl":"10.1007/s10856-026-07036-z","url":null,"abstract":"<div><p>Ischemia–reperfusion injury (IRI) continues to be a significant obstacle in liver transplantation and often results in graft dysfunction and suboptimal outcomes after transplantation. The current preservation solutions, such as the University of Wisconsin (UW) solution, provide limited protection against oxidative stress, inflammation, and apoptosis. The present study was undertaken to design and test a bioactive preservation solution supplemented with resveratrol-loaded chitosan nanoparticles (Resv-CNPs) and adipose-derived stem cell conditioned medium (ASC-CM) to achieve hepatoprotection during IRI. Resv-CNPs were synthesized using the ionic gelation method and characterized for encapsulation efficiency, release profile, and antioxidant activity. HepG2 cells were exposed to an in vitro IRI model, which involved 24h cold ischemia in UWCM solution followed by 48h reperfusion. Cell viability was evaluated by MTT assay, and ALT, AST, LDH, HMGB1, IL-1β, and IL-6 were measured by ELISA. An in vivo rat model was used to validate the hepatic preservation effect after 24h of cold storage in different solutions. Histopathological changes were assessed by H&amp;E staining and Suzuki scoring. Resv-CNPs had an encapsulation efficiency of 46.21±7.12%, and it showed a biphasic release pattern. The drug release from nanoparticles could provide sustained release of resveratrol for up to 72h. Free resveratrol and Resv-CNPs showed excellent DPPH radical scavenging activity. UWCM supplemented with Resv-CNPs dose-dependently increased hepatocyte viability and significantly decreased injury and inflammatory markers compared to free resveratrol and blank nanoparticles in vitro. In vivo, livers preserved with UWCM + Resv-CNPs showed decreased necrosis, reduced liver injury markers, better architecture, and lower Suzuki scores. The incorporation of Resv-CNPs and ASC-CM into the UW solution provided strong antioxidant, anti-inflammatory, and anti-apoptotic protection against hepatic IRI. This next-generation preservation formulation may improve graft quality, extend storage time, and have the potential to enhance transplantation outcomes.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13529896/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147969473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and application of modified peach gum polysaccharide in the repair of central nervous system injury","authors":"An Wang,&nbsp;Zhangheng Huang,&nbsp;Haoqi Chen,&nbsp;Qiqiang Xing,&nbsp;Jing Wang,&nbsp;Peiling Dai,&nbsp;Wangying Dai","doi":"10.1007/s10856-026-07059-6","DOIUrl":"10.1007/s10856-026-07059-6","url":null,"abstract":"<div><p>Spinal cord injury (SCI) is an irreversible neurological injury that leads to severe motor dysfunction and neurological deficiencies, imposing a large social load. Due to the difficulty of the SCI procedure, the prognosis is normally bad. In recent years, naturally derived hydrogels have garnered increasing attention. Peach gum (PG) primarily consists of macromolecular polysaccharides with abundant hydroxyl and carboxyl groups on its branched chains, making it amenable to modification. This study is the first to prepare carboxymethylated peach gum polysaccharide (CPG) using PG as the raw material and load it with nerve growth factor (NGF) to improve the adverse microenvironment post-SCI and promote axonal regeneration. Carboxymethylation modification of PG was done, and its effective change was verified by infrared spectroscopy and degree of replacement measurements. After loading NGF, its characterization was examined. The mechanical characteristics, thermal behavior, and self-healing ability of CPG + NGF were shown to be outstanding in the results. Cell experiments demonstrated that, compared to the sham-operated group, CPG + NGF significantly promotes neurite outgrowth and development in PC-12 cells. Ultimately, CPG + NGF was implanted into the injured spinal cords of rats, and research demonstrated that CPG + NGF significantly improves the microenvironment of the affected areas, fosters axonal regeneration in severely damaged spinal cord regions, and enhances motor function in the rats. The modified PG biopolymer scaffold developed in this study exhibits superior mechanical strength, excellent cytocompatibility, and enhanced biological functionality. In summary, this research provides new insights into the application of biomacromolecules in SCI repair.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13395878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced osteogenesis and antibacterial activity of bioactive glass-coated, epsilon-poly-L-lysine-loaded, polycaprolactone 3D-printed scaffolds with potential use in bone tissue engineering.","authors":"Eddy Shan, Liang Feng, Elena Figuero, Mariano Sanz, Maurizio Tonetti, Jinwu Wang","doi":"10.1007/s10856-026-07065-8","DOIUrl":"https://doi.org/10.1007/s10856-026-07065-8","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the structural properties and biological in vitro performance of 3D-printed polycaprolactone (PCL) scaffolds, coated with bioactive glass (BG) and functionalized with epsilon-poly-L-lysine (EPL) when co-cultured with rat bone marrow mesenchymal stem cells.</p><p><strong>Material and methods: </strong>The physicochemical characterization included scanning electron microscopy, energy dispersive spectroscopy, x-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and degradation kinetics. The biological characterization assessed antibacterial activity using a disk diffusion assay, cell viability of rat bone marrow mesenchymal stem cells with confocal laser scanning microscopy, cell proliferation and cytotoxicity with tetrazolium assay and relative gene and protein expression through reverse transcriptase quantitative polymerase chain reaction and western blot.</p><p><strong>Results: </strong>The physicochemical characterization showed a successful and homogeneous distribution of the ceramic component and confirmed EPL-loading. The PCL-BG-EPL scaffolds demonstrated antibacterial activity against Staphylocccus aureus for up to 7 days. Cell proliferation was significantly higher for PCL-BG and PCL-BG-EPL scaffolds, but cytotoxicity also increased in both groups when comparing day 3 and day 7. The osteogenic potential was enhanced for BG-coated scaffolds, with an upregulation of Alpl, Opn, and Col1a1 genes when compared to controls. However, no significant differences were detected for protein synthesis among the three groups.</p><p><strong>Conclusions: </strong>Composite scaffolds exhibited favorable technical properties and superior biological performance over pure PCL scaffolds.</p>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":" ","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADA-GEL composite hydrogel films incorporating mesoporous bioactive glass nanoparticles and silver-doped bioactive glass nanoparticles for biomedical applications","authors":"Asimenia Lekidou,&nbsp;Thomas Papatasos,&nbsp;Qaisar Nawaz,&nbsp;Xanthippi Chatzistavrou,&nbsp;Aldo R. Boccaccini","doi":"10.1007/s10856-026-07057-8","DOIUrl":"10.1007/s10856-026-07057-8","url":null,"abstract":"<div><p>Hydrogels made from natural polymers are widely used in biomedical engineering as they resemble the extracellular matrix. Alginate dialdehyde-gelatin (ADA-GEL) hydrogels are notable for their biocompatibility, biodegradability, and adjustable crosslinking. However, their limited mechanical strength and bioactivity present significant challenges for use in tissue regeneration and wound healing. To overcome these issues, this study explores the addition of 0.1 and 1 wt.% mesoporous bioactive glass nanoparticles (MBGNs) or silver-doped bioactive glass nanoparticles (Ag-BGNs) into ADA-GEL hydrogels to create composite hydrogel films. These nanocomposites were tested in terms of printability, swelling, degradation, antibacterial effects, and in vitro compatibility using MG-63 cells (osteosarcoma cells). MBGNs improved the hydrogels’ osteogenic ability and structural stability. At the same time, Ag-BGNs provided vigorous antibacterial activity, especially against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>, without significantly affecting cell viability and morphology. The use of bioactive and antimicrobial nanoparticles in the ADA-GEL matrix offers a promising approach for developing new soft biomaterials for applications such as bone regeneration, wound healing, and implant coatings.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":647,"journal":{"name":"Journal of Materials Science: Materials in Medicine","volume":"37 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10856-026-07057-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147832036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}