Unraveling the immunomodulatory and metabolic effects of bioactive glass S53P4 on macrophages in vitro

IF 4.5 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2025-01-24 DOI:10.1007/s10856-025-06861-y

Karoliina Kajander, Nicole Nowak, Negin Vaziri, Pekka K. Vallittu, Terhi J. Heino, Jorma A. Määttä

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引用次数: 0

Abstract

Macrophage metabolism is closely linked to their phenotype and function, which is why there is growing interest in studying the metabolic reprogramming of macrophages. Bioactive glass (BG) S53P4 is a bioactive material used especially in bone applications. Additionally, BG S53P4 has been shown to affect macrophages, but the mechanisms through which the possible immunomodulatory effects are conveyed remain unclear. According to the results presented here, the lipopolysaccharide (LPS) induced suppression in oxidative phosphorylation is rescued in macrophages cultured with BG S53P4 before the inflammatory stimulus. Additionally, BG S53P4-exposed macrophages expressed lower mRNA levels of inflammatory cytokines Il6 and Il1b, as well as demonstrated decreased activation of inflammatory interferon regulatory factor (IRF) and NF-κB pathways and nitrogen oxide secretion in response to LPS. These results did not rely on cells being in direct contact with the material as similar effects were observed in the presence of BG S53P4-conditioned medium. Our findings link the immunomodulatory properties of BG S53P4 and macrophage metabolism, which improves our understanding of the mechanisms underlying the clinical efficacy of bioactive glasses.

Graphical Abstract

查看原文本刊更多论文

体外研究生物活性玻璃S53P4对巨噬细胞的免疫调节和代谢作用。

巨噬细胞的代谢与其表型和功能密切相关，因此对巨噬细胞代谢重编程的研究日益引起人们的兴趣。生物活性玻璃（BG） S53P4是一种生物活性材料，尤其用于骨应用。此外，BG S53P4已被证明可影响巨噬细胞，但其可能的免疫调节作用的传递机制尚不清楚。根据本研究结果，在炎症刺激前，脂多糖（LPS）诱导的氧化磷酸化抑制在BG S53P4培养的巨噬细胞中被恢复。此外，BG s53p4暴露的巨噬细胞表达较低的炎症细胞因子Il6和Il1b mRNA水平，炎症干扰素调节因子（IRF）和NF-κB通路的激活以及一氧化氮分泌在LPS的作用下下降。这些结果并不依赖于细胞与材料的直接接触，因为在BG s53p4条件培养基的存在下观察到类似的效果。我们的研究结果将BG S53P4的免疫调节特性与巨噬细胞代谢联系起来，从而提高了我们对生物活性眼镜临床疗效机制的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.