Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis

IF 4.5 3区 医学 Q2 ENGINEERING, BIOMEDICAL
Paula Milena Giraldo-Osorno, Adam Benedict Turner, Sebastião Mollet Barros, Robin Büscher, Simone Guttau, Farah Asa’ad, Margarita Trobos, Anders Palmquist
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Abstract

Implants aim to restore skeletal dysfunction associated with ageing and trauma, yet infection and ineffective immune responses can lead to failure. This project characterized the microbiological and host cell responses to titanium alloy with or without electroplated metallic copper. Bacterial viability counting and scanning electron microscopy quantified and visualized the direct and indirect bactericidal effects of the Cu-electroplated titanium (Cu-Ep-Ti) against two different Staphylococcus aureus strains. Human THP-1 macrophage adhesion and viability was analyzed, along with phagocytosis. Results showed potent antimicrobial activity alongside promising host-immunomodulatory properties. Direct and indirect exposure to Cu-Ep-Ti produced potent bactericidal effects resulting in 94–100% reductions in bacterial viability at 24 h, with complete eradication in some cases. As expected, cytotoxicity was observed in THP-1 macrophages without media exchange, though when media was exchanged at 8, 24 and 48 h cell viability was equivalent to Control-Ti. Interestingly macrophages adhered to the copper material or grown in the presence of copper ions showed 7-fold increase in phagocytosis of S. aureus bioparticles compared to Control-Ti, suggesting a dual bactericidal and host immunomodulatory mechanism. In conclusion, this Cu-electroplated Ti biomaterial can limit bacterial contamination on the implant surface, whilst simultaneously promoting a beneficial antimicrobial immune response.

Graphical Abstract

阳极氧化Ti6Al4V-ELI,镀铜,对金黄色葡萄球菌具有杀菌作用,增强巨噬细胞的吞噬作用。
植入物旨在恢复与衰老和创伤相关的骨骼功能障碍,但感染和无效的免疫反应可能导致失败。本项目研究了镀铜或不镀铜的钛合金对微生物和宿主细胞的反应。细菌活力计数和扫描电镜定量和可视化了铜电镀钛(Cu-Ep-Ti)对两种不同金黄色葡萄球菌菌株的直接和间接杀菌效果。分析人THP-1巨噬细胞的粘附和活力,以及吞噬作用。结果显示,它具有强大的抗菌活性,并具有良好的宿主免疫调节特性。直接和间接暴露于Cu-Ep-Ti产生了强有力的杀菌作用,导致24小时细菌活力降低94-100%,在某些情况下完全根除。正如预期的那样,在没有介质交换的THP-1巨噬细胞中观察到细胞毒性,尽管在8、24和48 h交换介质时,细胞活力与对照ti相当。有趣的是,与Control-Ti相比,粘附在铜材料上或在铜离子存在下生长的巨噬细胞对金黄色葡萄球菌生物颗粒的吞噬能力增加了7倍,这表明巨噬细胞具有双重杀菌和宿主免疫调节机制。总之,这种镀铜钛生物材料可以限制种植体表面的细菌污染,同时促进有益的抗菌免疫反应。
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来源期刊
Journal of Materials Science: Materials in Medicine
Journal of Materials Science: Materials in Medicine 工程技术-材料科学:生物材料
CiteScore
8.00
自引率
0.00%
发文量
73
审稿时长
3.5 months
期刊介绍: The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.
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