Progress in Biophysics & Molecular Biology最新文献_第5页

From gene editing to tumor eradication: The CRISPR revolution in cancer therapy 从基因编辑到肿瘤根除：癌症治疗中的CRISPR革命

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-04-16 DOI: 10.1016/j.pbiomolbio.2025.04.003

Ashiq Ali , Urooj Azmat , Aisha Khatoon , Kaynaat Akbar , Bilal Murtaza , Ziyi Ji , Urooj Irshad , Zhongjing Su

{"title":"From gene editing to tumor eradication: The CRISPR revolution in cancer therapy","authors":"Ashiq Ali , Urooj Azmat , Aisha Khatoon , Kaynaat Akbar , Bilal Murtaza , Ziyi Ji , Urooj Irshad , Zhongjing Su","doi":"10.1016/j.pbiomolbio.2025.04.003","DOIUrl":"10.1016/j.pbiomolbio.2025.04.003","url":null,"abstract":"<div><div>Cancer continues to be a significant worldwide health concern, characterized by high rates of occurrence and death. Unfortunately, existing treatments frequently fall short of delivering satisfying therapeutic outcomes. Immunotherapy has ushered in a new era in the treatment of solid tumors, yet its effectiveness is still constrained and comes with unwanted side effects. The advancement of cutting-edge technology, propelled by gene analysis and manipulation at the molecular scale, shows potential for enhancing these therapies. The advent of genome editing technologies, including CRISPR-Cas9, can greatly augment the efficacy of cancer immunotherapy. This review explores the mechanism of CRISPR-Cas9-mediated genome editing and its wide range of tools. The study focuses on analyzing the effects of CRISPR-induced double-strand breaks (DSBs) on cancer immunotherapy, specifically by gene knockdown or knockin. In addition, the study emphasizes the utilization of CRISPR-Cas9-based genome-wide screening to identify targets, the potential of spatial CRISPR genomics, and the extensive applications and difficulties of CRISPR-Cas9 in fundamental research, translational medicine, and clinical environments.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"196 ","pages":"Pages 114-131"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myelin: A possible proton capacitor for energy storage during sleep and energy supply during wakefulness 髓磷脂：一种可能的质子电容器，用于睡眠时的能量储存和清醒时的能量供应。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-03-28 DOI: 10.1016/j.pbiomolbio.2025.03.001

Alessandro Maria Morelli , Ann Saada , Felix Scholkmann

引用次数: 0

Advances in the study of epithelial mesenchymal transition in cancer progression: Role of miRNAs 癌症进展中上皮间充质转化的研究进展：miRNAs 的作用。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-04-02 DOI: 10.1016/j.pbiomolbio.2025.04.001

Jia Zhang , Runting Yin , Yongwang Xue , Rong Qin , Xuequan Wang , Shuming Wu , Jun Zhu , Yan-Shuang Li , Cai Zhang , Yuan Wei

{"title":"Advances in the study of epithelial mesenchymal transition in cancer progression: Role of miRNAs","authors":"Jia Zhang , Runting Yin , Yongwang Xue , Rong Qin , Xuequan Wang , Shuming Wu , Jun Zhu , Yan-Shuang Li , Cai Zhang , Yuan Wei","doi":"10.1016/j.pbiomolbio.2025.04.001","DOIUrl":"10.1016/j.pbiomolbio.2025.04.001","url":null,"abstract":"<div><div>Epithelial-mesenchymal transition (EMT) has been extensively studied for its roles in tumor metastasis, the generation and maintenance of cancer stem cells and treatment resistance. Epithelial mesenchymal plasticity allows cells to switch between various states within the epithelial-mesenchymal spectrum, resulting in a mixed epithelial/mesenchymal phenotypic profile. This plasticity underlies the acquisition of multiple malignant features during cancer progression and poses challenges for EMT in tumors. MicroRNAs (miRNAs) in the microenvironment affect numerous signaling processes through diverse mechanisms, influencing physiological activities. This paper reviews recent advances in EMT, the role of different hybrid states in tumor progression, and the important role of miRNAs in EMT. Furthermore, it explores the relationship between miRNA-based EMT therapies and their implications for clinical practice, discussing how ongoing developments may enhance therapeutic outcomes.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"196 ","pages":"Pages 69-90"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell detachment: A review of techniques, challenges, and opportunities for advancing biomedical research and applications 细胞分离：关于推进生物医学研究和应用的技术、挑战和机遇的综述。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-02-27 DOI: 10.1016/j.pbiomolbio.2025.02.004

Polina Vertegel , Pavel Milkin , Anton Murashko , Mikhail Parker , Kristina Peranidze , Natalia Emashova , Sergiy Minko , Vladimir Reukov

{"title":"Cell detachment: A review of techniques, challenges, and opportunities for advancing biomedical research and applications","authors":"Polina Vertegel , Pavel Milkin , Anton Murashko , Mikhail Parker , Kristina Peranidze , Natalia Emashova , Sergiy Minko , Vladimir Reukov","doi":"10.1016/j.pbiomolbio.2025.02.004","DOIUrl":"10.1016/j.pbiomolbio.2025.02.004","url":null,"abstract":"<div><div>Culturing living cells outside the body is a complex process involving various techniques. Despite advances, harvesting cells remains challenging, especially in light of new emerging and scaled-up cell culture technologies. Enzymatic adherent cell harvesting is the most used and robust technology but can harm cells. Non-enzymatic detachment methods offer advantages but also present challenges. Thermo-responsive polymers require precise control of the molecular characteristics and thickness of the thermoresponsive films, which makes this method less robust and more expensive. This review highlights the importance of controlling harvested cell quality and its relationship to cell binding and detachment mechanisms. Many alternative methods have not been extensively analyzed, and their impact on cell quality beyond standard viability assays is not yet known. Developing robust cell harvesting methods for bioreactor microcarriers is a rapidly growing challenge as the cell manufacturing industry expands. Microcarriers with stimuli-responsive coatings face challenges similar to those observed for laboratory-scale cell dishes and bring an additional aspect of the need for microbead recycling consideration. All that together underlines the importance of the research in biomaterials and biotechnology for cell manufacturing.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"196 ","pages":"Pages 50-68"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular senescence as a key player in chronic heart failure pathogenesis: Unraveling mechanisms and therapeutic opportunities 细胞衰老在慢性心力衰竭发病机制中的关键作用：揭示机制和治疗机会。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-02-15 DOI: 10.1016/j.pbiomolbio.2025.02.002

Shuqing Zhao , Yu Zhang , Ying Zhao , Xiaohui Lu

引用次数: 0

The key role of Piezo1 channels in ferroptosis after spinal cord injury and the therapeutic potential of Piezo1 inhibitors Piezo1通道在脊髓损伤后铁下垂中的关键作用及Piezo1抑制剂的治疗潜力

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-05-06 DOI: 10.1016/j.pbiomolbio.2025.05.001

Qianxi Li, Chenyu Li, Xinyu Liu, Zixuan Guo, Xinxin Li, Xin Zhang

{"title":"The key role of Piezo1 channels in ferroptosis after spinal cord injury and the therapeutic potential of Piezo1 inhibitors","authors":"Qianxi Li, Chenyu Li, Xinyu Liu, Zixuan Guo, Xinxin Li, Xin Zhang","doi":"10.1016/j.pbiomolbio.2025.05.001","DOIUrl":"10.1016/j.pbiomolbio.2025.05.001","url":null,"abstract":"<div><h3>Background</h3><div>Ferroptosis has been confirmed to be one of the key mechanisms of neuronal injury and dysfunction after spinal cord injury (SCI). Mechanical stresses such as deformation, compression, and stretching not only directly cause physical damage to spinal cord tissue at the moment of SCI, but also promote the development of ferroptosis through various pathways. However, the mechanism of ferroptosis after SCI remains unclear, which hinders the development of therapeutic methods.</div></div><div><h3>Objective</h3><div>This article aims to review the key mechanisms by which mechanical stress affects ferroptosis after SCI, including its impact on the structure and function of the endoplasmic reticulum (ER) and mitochondria, its role in triggering inflammatory responses, and its activation of mechanosensitive channels. Special emphasis is placed on the role of Piezo1 channels, which are key factors in cell mechanosensation and ion homeostasis regulation. The review explores how Piezo1 channels are upregulated by mechanical stress after SCI and participate in the ferroptosis process by mediating ion flow and other mechanisms.</div></div><div><h3>Conclusions</h3><div>Inhibiting Piezo1 channels may be a potential therapeutic strategy for SCI. This review summarizes the therapeutic potential of Piezo1 inhibitors by sorting out existing studies, hoping to provide a theoretical basis for effective therapeutic strategies targeting ferroptosis after SCI.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"196 ","pages":"Pages 132-140"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing targeted therapies in pancreatic cancer: Leveraging molecular abberrations for therapeutic success 推进胰腺癌靶向治疗：利用分子畸变获得治疗成功。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-06-01 Epub Date: 2025-02-21 DOI: 10.1016/j.pbiomolbio.2025.02.003

Tanvi Gupta , Mohd Murtaza

{"title":"Advancing targeted therapies in pancreatic cancer: Leveraging molecular abberrations for therapeutic success","authors":"Tanvi Gupta , Mohd Murtaza","doi":"10.1016/j.pbiomolbio.2025.02.003","DOIUrl":"10.1016/j.pbiomolbio.2025.02.003","url":null,"abstract":"<div><div>Pancreatic cancer is one of the most deadly with poor prognosis and overall survival rate due to the dense stroma in the tumors which often is challenging for the delivery of drug to penetrate deep inside the tumor bed and usually results in the progression of cancer. The conventional treatment such as chemotherapy, radiotherapy or surgery shows a minimal benefit in the survival due to the drug resistance, poor penetration, less radiosensitivity or recurrence of tumor. There is an urgent demand to develop molecular-level targeted therapies to achieve therapeutic efficacy in the pancreatic ductal adenocarcinoma (PDAC) patients. The precision oncology focuses on the unique attributes of the patient such as epigenome, proteome, genome, microbiome, lifestyle and diet habits which contributes to promote oncogenesis. The targeted therapy helps to target the mutated proteins responsible for controlling growth, division and metastasis of tumor in the cancer cells. It is very important to consider all the attributes of the patient to provide the suitable personalized treatment to avoid any severe side effects. In this review, we have laid emphasis on the precision medicine; the utmost priority is to improve the survival of cancer patients by targeting molecular mutations through transmembrane proteins, inhibitors, signaling pathways, immunotherapy, gene therapy or the use of nanocarriers for the delivery at the tumor site. It will become beneficial therapeutic window to be considered for the advanced stage pancreatic cancer patients to prolong their survival rate.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"196 ","pages":"Pages 19-32"},"PeriodicalIF":3.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vinculin: A new target for the diagnosis and treatment of disease 长春素：疾病诊断和治疗的新靶点。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-03-01 Epub Date: 2025-01-23 DOI: 10.1016/j.pbiomolbio.2025.01.004

Jiqiang Li , Weiming Wang , Zipeng Lin , Zhenyu Liu , Ruilin Zhang , Runwen Li , Jie Zhang , Youkun Zheng , Dalian Qin , Ya Wu , Yong Liu

引用次数: 0

Electrical impedance sensing in stem cell research: Insights, applications, and future directions 干细胞研究中的电阻抗传感：见解、应用和未来方向。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-03-01 Epub Date: 2024-11-16 DOI: 10.1016/j.pbiomolbio.2024.11.004

Hassan Moghtaderi , Saeed Mohahammadi , Golfam Sadeghian , Mahua Choudhury , Ahmed Al-Harrasi , Shaikh Mizanoor Rahman

{"title":"Electrical impedance sensing in stem cell research: Insights, applications, and future directions","authors":"Hassan Moghtaderi , Saeed Mohahammadi , Golfam Sadeghian , Mahua Choudhury , Ahmed Al-Harrasi , Shaikh Mizanoor Rahman","doi":"10.1016/j.pbiomolbio.2024.11.004","DOIUrl":"10.1016/j.pbiomolbio.2024.11.004","url":null,"abstract":"<div><div>The exceptional differentiation abilities of stem cells make them ideal candidates for cell replacement therapies. Considering their great potential, researchers should understand how stem cells interact with other cell types. The production of high-quality differentiated cells is crucial for favorable treatment and makes them an ideal choice for clinical applications. Label-free stem cell monitoring approaches are anticipated to be more effective in this context, as they ensure quality of differentiation while preserving the therapeutic potential. Electric cell-substrate impedance sensing (ECIS) is a nonintrusive technique that enables cell quantification through continuous monitoring of adherent cell behavior using electronic transcellular impedance measurements. This technique also facilitates the study of cell growth, motility, differentiation, drug effects, and cell barrier functions. Therefore, numerous studies have identified ECIS as an effective method for monitoring stem cell quality and differentiation. In this review, we discuss the current understanding of ECIS's achievements in examining cell behaviors and the potential applications of ECIS arrays in preclinical stem cell research. Moreover, we highlight our present knowledge concerning ECIS's contributions in examining cell behaviors and speculate about the future uses of ECIS arrays in preclinical stem cell research. This review also aims to stimulate research on electrochemical biosensors for future applications in regenerative medicine.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"195 ","pages":"Pages 1-14"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142669793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming agents: The power of structural modifications in glioblastoma multiforme therapy 转化剂：结构改变在胶质母细胞瘤多形性治疗中的作用。

IF 3.2 3区生物学

Progress in Biophysics & Molecular Biology Pub Date : 2025-03-01 Epub Date: 2024-12-17 DOI: 10.1016/j.pbiomolbio.2024.12.001

Seyedeh Elham Norollahi , Saman Morovat , Arman Keymoradzadeh , Arman Hamzei , Morteza Modaeinama , Nazanin Soleimanmanesh , Yasaman Soleimanmanesh , Ali Najafizadeh , Elahe Bakhshalipour , Babak alijani , Ali Akbar Samadani

{"title":"Transforming agents: The power of structural modifications in glioblastoma multiforme therapy","authors":"Seyedeh Elham Norollahi , Saman Morovat , Arman Keymoradzadeh , Arman Hamzei , Morteza Modaeinama , Nazanin Soleimanmanesh , Yasaman Soleimanmanesh , Ali Najafizadeh , Elahe Bakhshalipour , Babak alijani , Ali Akbar Samadani","doi":"10.1016/j.pbiomolbio.2024.12.001","DOIUrl":"10.1016/j.pbiomolbio.2024.12.001","url":null,"abstract":"<div><div>Glioblastoma (GBM) is a very deadly type of brain tumor with a poor prognosis and a short survival rate. Recent advancements in understanding GBM's molecular and genetic characteristics have led to the development of various therapeutic and diagnostic strategies. Key elements such as microRNAs, lncRNAs, exosomes, angiogenesis, and chromatin modifications are highlighted, alongside significant epigenetic alterations that impact therapy and diagnosis. Despite these advancements, molecular classifications have not improved patient outcomes due to intratumoral diversity complicating targeted therapies. In this article, it is tried to emphasize the potential of investigating the epigenetic landscape of GBM, particularly identifying patients with diffuse hypermethylation at gene promoters associated with better outcomes. Integrating epigenetic and genetic data has enhanced the identification of glioma subtypes with high diagnostic precision. The reversibility of epigenetic changes offers promising therapeutic prospects, as recent insights into the “epigenetic orchestra” suggest new avenues for innovative treatment modalities for this challenging cancer. In this review article, we focus on the roles of translational elements and their alterations in the context of GBM diagnosis and therapy.</div></div>","PeriodicalId":54554,"journal":{"name":"Progress in Biophysics & Molecular Biology","volume":"195 ","pages":"Pages 41-56"},"PeriodicalIF":3.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0