Food Hygiene and Safety Science最新文献

{"title":"[Effect of Peracetic Acid Formulation on Hygiene Indicator Bacteria in Cut Salads].","authors":"Buren Qiqige, Taisuke Yone, Ayaka Nakamura, Takashi Kuda, Hajime Takahashi","doi":"10.3358/shokueishi.66.25","DOIUrl":"10.3358/shokueishi.66.25","url":null,"abstract":"<p><p>The study aimed to evaluate the effect of peracetic acid formulation (PAA) in the cut salads as a model food with different treatment conditions and observed their effect on the shelf life. This study revealed that at 80 ppm PAA, the standard for use on vegetables, showed a temporary and weak bactericidal effect. Additionally, there was no improvement in the shelf life of the cut salad. In contrast, PAA treatment at 240 ppm significantly reduced and suppressed the growth of hygiene indicator bacteria for 7 days. Moreover, no injured bacteria were produced due to proper sterilization. Based on these findings, it is believed that 240 ppm of PAA is suitable for washing cut salads and effective to improve their shelf life.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 2","pages":"25-31"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Validation of High-Sensitivity Analysis for Chloropropanols and Related Substances in Infant Formula].","authors":"Yudai Iwabe, Eisuke Toriumi, Mikihiko Yoshida, Kazushi Mizukoshi, Tomoji Igarashi","doi":"10.3358/shokueishi.66.19","DOIUrl":"10.3358/shokueishi.66.19","url":null,"abstract":"<p><p>Chloropropanols and related substances have received a great deal of attention in the world. Regulation values have already been established in the EU for infant formula. There are several validated analytical methods for these substances, such as the AOAC official method. However, no analytical official method has been established for infant formula in Japan. In this study, the AOAC official method was improved to a more versatile splitless injection method in order to make it feasible for many laboratories. The results of the validation data for the improved method with optimized injection conditions were as follows. The limit of quantification was 10 μg/kg, the recovery rate ranged from 81.6 to 114.5%, and the intermediate precision ranged from 1.6 to 7.6%. These results were adapt to AOAC and EU requirement and demonstrated the good validity of the improved method.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 1","pages":"19-24"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Validation Study on the Developed Method for Cyanide in Various Mineral Waters].","authors":"Hiroshi Sakuragi, Masako Suzuki, Hiroyuki Ohno","doi":"10.3358/shokueishi.66.46","DOIUrl":"https://doi.org/10.3358/shokueishi.66.46","url":null,"abstract":"<p><p>A method for the determination of two kinds of cyanide, namely cyanide ion and cyanogen chloride, in various mineral waters (MWs) has been developed and validated. MWs are varying in the degree of hardness and carbonation. The results of two carbonated MWs obtained through the validated official method for cyanide analysis in tap water did not meet the criteria of the validation guideline for MW in Japan. Furthermore, in the pH adjustment during pretreatment, some MWs did not reach approximately pH 2.4, the level assumed in tap water quality tests. After five-fold dilution of the samples with phosphate buffer, the specified pH value of 2.4 was achieved, leading to the improvements in trueness. The validation tests of the method with the additional dilution step showed the trueness from 93.0 to 103.8%, RSDs of repeatability and within-laboratory reproducibility below 3.83% and 4.68%, respectively. These values fulfill the criteria on both analytes. The developed method indicated the potential applicability for accurate determination of cyanide ion and cyanogen chloride in a range of commercially available MW products.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 3","pages":"46-50"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144978971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and Performance Evaluation of an Analytical Method for Resorcylic Acid Lactones in Bovine Urine for Monitoring Japanese Beef Exports to the European Union].","authors":"Chiharu Takagi, Ayumu Nakamura, Youichi Kouno, Rie Ito, Tomoaki Tsutsumi, Hiroshi Akiyama, Shizuka Saito-Shida","doi":"10.3358/shokueishi.66.32","DOIUrl":"https://doi.org/10.3358/shokueishi.66.32","url":null,"abstract":"<p><p>A quantitative method for determining resorcylic acid lactones (zeranol, taleranol, zearalanone, and zearalenone) in bovine urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and evaluated for use in the monitoring of Japanese beef exports to the European Union. This method involves the hydrolysis of conjugates by ß-glucuronidase/aryl sulfatase, clean-up with an immunoaffinity cartridge, and analysis using LC-MS/MS. The developed method was evaluated for zeranol, taleranol, zearalanone, and zearalenone in bovine urine at concentrations of 1 and 2 μg/L. The trueness ranged from 92 to 101%, with intra-day precision of <9% and inter-day precision of <13%. No interfering peaks were observed in the chromatogram near the analyte retention times. This analytical method is valuable for monitoring taleranol, zearalanone, and zearalenone in beef exports to the European Union. The proposed method is also applicable for screening whether zeranol was illegally administered or ingested via feed contaminated with Fusarium toxins, particularly in cases in which taleranol was detected.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 2","pages":"32-38"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Validation Study on a Rapid and Low-priced Method for Determination of Chloroacetic Acids in Mineral Water Products].","authors":"Atsuki Ozawa, Takanari Wakayama, Hiroyuki Ohno","doi":"10.3358/shokueishi.66.51","DOIUrl":"https://doi.org/10.3358/shokueishi.66.51","url":null,"abstract":"<p><p>An LC-MS/MS method for the simultaneous determination of chloroacetic acid, dichloroacetic acid and trichloroacetic acid (chloroacetic acids) in mineral water (MW) products was developed by modifying the Japanese official method to determine chloroacetic acids. To prevent ion suppression or enhancement caused by sample matrix in LC-MS/MS analysis, the Japanese official method comprises the cleanup step of analytes using cation exchange resin column. The step is time-consuming and costly, and thus the modified method adopted small-volume injection instead of it to prevent matrix effects on ionization, which enabled rapid analysis at low cost. A validation study was performed on the method using MW products which vary in the degree of hardness and contents of carbonate. The trueness, repeatability and reproducibility of the method were estimated to be within the ranges of 95.3 to 104.3%, 1.1 to 2.9% and 2.4 to 6.4%, respectively. The values of every performance parameter met the criteria in the guidelines announced by the Ministry of Health, Labour and Welfare of Japan. A recovery study was performed on 30 kinds of MW products to examine the applicability of the method, and the recovery rate of all target substances ranged from 91 to 108%. Therefore, the modified method is considered to be suitable for the determination of chloroacetic acids in MW products.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 3","pages":"51-55"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144978987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Examination of Simultaneous Extraction Method of 19 Food Additives in Processed Foods].","authors":"Tomoki Igarashi, Takahiro Sasaki, Mari Morikawa, Keiko Ushiyama, Chigusa Kobayashi, Yukiko Yamajima, Yuki Sadamasu, Kenji Otsuka","doi":"10.3358/shokueishi.66.1","DOIUrl":"10.3358/shokueishi.66.1","url":null,"abstract":"<p><p>In this study, we developed a simultaneous extraction method for 19 food additives, including sweeteners, preservatives, and antioxidants, to enhance testing efficiency across a wide range of processed foods. Samples underwent 2 or 4 extractions with acetonitrile containing 0.05 w/v% ascorbic acid palmitate, facilitated by the addition of water, phosphoric acid, magnesium sulfate, and sodium chloride. The extracts were then diluted and analyzed using instruments tailored to each specific compound. Recovery tests (n=5 or n=3) on 22 samples, encompassing high-protein, oily, and powdered foods, demonstrated satisfactory recovery rates between 76.6% and 111.6% (RSD: 0.1-6.6%). To validate the method, two concentrations-corresponding to the lower limit of quantification and the maximum permissible level-were added to two or three different samples per additives. This validation was conducted by a single analyst over two parallel runs across five days. The results indicated that the lower limit of quantification achieved trueness of 81.7-102.4%, repeatability of 0.3-6.3%, and within-laboratory reproducibility of 1.0-9.7%. Similarly, for the maximum level of use, trueness ranged from 80.6 to 105.5%, repeatability from 0.4 to 2.8%, and within-laboratory reproducibility from 0.6 to 4.3%, all meeting the target criteria. The developed method proves to be a valuable analytical tool, significantly enhancing the efficiency of food additive analysis.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 1","pages":"1-11"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Single-laboratory Validation Study of an Improved Multi-residue Method for Pesticide Residues in Green Tea Leaves Using LC-MS/MS].","authors":"Tomomi Takada, Sanae Tomizawa, Takushi Fujiwara, Kyoko Kamijo, Kazuoki Yamamoto, Yuri Saito, Yoshie Kokaji, Yoshihiro Ohsawa, Maiko Noguchi, Shoichi Tahara","doi":"10.3358/shokueishi.66.56","DOIUrl":"https://doi.org/10.3358/shokueishi.66.56","url":null,"abstract":"<p><p>In this study, a multi-residue method for pesticide residues in green tea leaves was developed using LC-MS/MS and the validity of the developed method was evaluated in accordance with Japanese guidelines. Based on the previously reported method, the amount of acetonitrile used for extraction, amount of hexane-washed distilled water added to the sample, and solid-phase column used for purification were investigated. In the developed method, the pesticides were extracted with 25 mL of acetonitrile after adding 10 mL of the hexane-washed distilled water to the sample and salted out. By cleaning the acetonitrile layer using Inert Sep^® PLS-3 (200 mg/20 mL) and Supelclean^TMENVI-Carb^TMII/PSA (300 mg, 600 mg/6 mL), the effect of the matrix on the green tea leaves was reduced. This method was validated by performing recovery tests at two concentrations (0.01 and 0.1 mg/kg) of 92 pesticides. Of the 92 pesticides, 84 met the criteria. The limit of quantification was 0.01 mg/kg, which is the uniform limit specified by the Food Sanitation Law of Japan. This study presents that the developed method is useful for the analysis of pesticide residues in green tea leaves.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 3","pages":"56-60"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144978994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of Decontamination Methods using Peracetic Acid for Shiga Toxin-producing Escherichia coli on the Surface of Beef Sub-primal Cuts].","authors":"Hajime Takahashi, Kaori Komori, Ayaka Nakamura, Fumiaki Koike, Takashi Kuda, Makoto Iwamoto, Jin Onodera, Jitsuo Mizowaki, Mitsushi Kobayashi","doi":"10.3358/shokueishi.66.39","DOIUrl":"https://doi.org/10.3358/shokueishi.66.39","url":null,"abstract":"<p><p>Peracetic acid formulation has received much attention in recent years as a surface disinfectant for beef. There are many reports on the bactericidal effect of peracetic acid formulation on beef surfaces. However, the application method of peracetic acid formulation aiming at the complete removal of Shiga toxin-producing Escherichia coli (STEC) from the surface of sub-primal cuts has not been evaluated. The objective of this study was to determine the condition under which peracetic acid formulation can be used to completely remove STEC from the surface of sub-primal cuts of beef.The effect of peracetic acid formulation was evaluated by immersing pieces of beef on whose surfaces E. coli O157:H7 were inoculated. To investigate the impact of peracetic acid concentration on bactericidal efficacy, small pieces (5 cm×5 cm×5 cm) of meat from five parts were immersed in peracetic acid at concentrations of 900 ppm and 1800 ppm for 30 min. The bacterial counts were decreased by 2.6-4.0 log CFU/cm² at 900 ppm, indicating a varying bactericidal effect depending on parts of beef. Conversely, at 1800 ppm, declines of 3.9-4.1 log CFU/cm² were observed, reaching the detection limit. To confirm the efficacy of peracetic acid formulation on the surface of sub-primal cuts, larger pieces (5 cm×20 cm×20 cm) of beef from nine parts were immersed in 1800 ppm peracetic acid for 30 min. As was the case with small pieces of beef, bacterial counts were decreased by 3.5-3.8 log CFU/cm² and reached the detection limit. Given the actual number of total viable bacteria on the surface of sub-primal cuts, the reduction in bacterial counts observed in this study is a condition that allows for complete removal of STEC from the surface of sub-primal cuts.Consequently, it can be concluded that the immersion in peracetic acid with a concentration of 1800 ppm for 30 min is the appropriate treatment to decontaminate STEC totally from the surface of sub-primal cuts of beef.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 3","pages":"39-45"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144978982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Ruhemann's Purple Monitoring by UHPLC/MS/MS for Ninhydrin Test].","authors":"Takashi Kurohara, Chiye Tatebe, Yumiko Fujiwara, Fuyuko Hioki, Shohei Takada, Atsuko Tada, Naoki Sugimoto","doi":"10.3358/shokueishi.66.12","DOIUrl":"10.3358/shokueishi.66.12","url":null,"abstract":"<p><p>For amino acids used as food additives, Japan's Specifications and Standards for Food Additives stipulate the ninhydrin test as an identification test. The ninhydrin test is a simple method that involves the visual determination of purple color from the formation of Ruhemann's purple (RP) and does not require special equipment, facilitating its widespread use in society. However, because of this background, objective and molecular selective observation methods for monitoring RP itself as an analyte have not been fully investigated. Therefore, in this study, a UHPLC/MS/MS method was developed to specifically monitor RP and support visual judgment. This method identified RP-derived fragment ions at m/z 170 (ESI (-)) and 133 (ESI (+)), which can be monitored in the multiple reaction monitoring mode and were shown to correlate with the intensity of the purple color. In addition, computational chemistry was applied to scientifically estimate the molecular structures of the fragment ions. In this study, we established a useful analytical method that complements the objectivity of the ninhydrin test. This method is also expected to be utilized for further optimization of test reaction conditions.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"66 1","pages":"12-18"},"PeriodicalIF":0.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143517276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment of Rapid Simultaneous Analysis Method for Plant Toxins by LC-TOF-MS].","authors":"Hideki Sato, Yoshinori Kawano, Shiho Tanaka, Junko Tsunematsu, Miki Matsunaga, Yoshihiro Miyao, Keiko Nakamuta","doi":"10.3358/shokueishi.65.7","DOIUrl":"10.3358/shokueishi.65.7","url":null,"abstract":"<p><p>Assuming food poisoning caused by toxic plants, an LC-TOF-MS-based method for the rapid and simultaneous analysis of 16 plant toxins was established. After adding water-methanol (1 : 9) and n-hexane, the samples were homogenized and extracted, and then subjected to centrifugal separation. Without any purification procedures, LC-TOF-MS measurements were performed, and qualitative and quantitative analyses using monoisotopic ion [M+H]+ (m/z) were conducted. The addition-recovery test using curry showed that qualitative analysis was possible under a setting with a retention time of ±0.2 minutes or less and mass accuracy of 5 ppm or lower and that quantitative analysis was possible with a recovery rate of 68-142% and a repeatability of 1.4-10.1%. Furthermore, measurements of the amount of plant toxins in the boiled plants and broths of cooked toxic plants demonstrated the transfer of plant toxins to broths. These suggest that in the event of food poisoning, broths may be used as an analysis sample, even when plants are not available.</p>","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 1","pages":"7-14"},"PeriodicalIF":0.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}