Food Hygiene and Safety Science最新文献

[Species Identification of the Genus Lagocephalus Using RFLP of PCR-amplified Fragments of the Cytochrome C Oxidase Subunit I (COI) Gene]. [利用细胞色素C氧化酶亚基I （COI）基因pcr扩增片段的RFLP鉴定Lagocephalus属植物]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.61

Tsuyoshi Ikehara, Haruki Tsujimoto, Naomasa Oshiro

{"title":"[Species Identification of the Genus Lagocephalus Using RFLP of PCR-amplified Fragments of the Cytochrome C Oxidase Subunit I (COI) Gene].","authors":"Tsuyoshi Ikehara, Haruki Tsujimoto, Naomasa Oshiro","doi":"10.3358/shokueishi.67.61","DOIUrl":"10.3358/shokueishi.67.61","url":null,"abstract":"In this study, we performed the applicability of the COI region (704 bp) for identification of seven Lagocephalus species (L. spadiceus, L. cheesemanii, L. inermis, L. lagocephalus, L. lunaris, L. sceleratus, and L. suezensis) distributed in Japanese Waters using PCR- restriction fragment length polymorphism (RFLP).Three restriction enzymes (DdeI, BanII and NlaIII) were used for the RFLP analysis. Based on the schematic diagram of the PCR-RFLP electrophoresis patterns obtained from the DNA sequence, a flowchart was created for identifying the seven species of the genus Lagocephalus. The species identification was based on digesting the PCR product of the gene with three restriction enzymes and confirming the agarose gel electrophoresis patterns of the cleaved fragments as mentioned on the flowchart. This method was applied to identify the source species of commercially available processed pufferfish products. The species of sampled Lagocephalus pufferfish (5 products, 141 specimens) were identified as L. spadiceus (4 products, 138 specimens) and L. cheesemanii (1 products, 3 specimen).These results show that the PCR-RFLP of the COI gene using the three restriction enzymes is a useful tool for the species identification of commercially distributed processed pufferfish products.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"67 2","pages":"61-66"},"PeriodicalIF":0.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148320579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Distribution Status of Genetically Modified Soybeans from Brazil to Japan Produced in 2021 and 2022]. 【2021年和2022年巴西对日转基因大豆分布情况】。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.74

Keisuke Soga, Yu Hashimoto, Tomohiro Egi, Chie Taguchi, Satoko Yoshiba, Norihito Shibata, Reona Takabatake

引用次数: 0

[Surveillance of the Levels of Naturally Derived Benzoic Acid in Fresh Foods①Comparison of Survey Results and Analytical Methods]. [新鲜食品中天然苯甲酸含量监测①调查结果与分析方法比较]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.67

Yu Shiozawa, Rio Suzuki, Shui Watanabe, Ayako Ito, Rina Nakamura, Toshiko Shimoi, Yukiko Yamajima, Narue Sakamaki, Kenji Otsuka, Maki Kanda

{"title":"[Surveillance of the Levels of Naturally Derived Benzoic Acid in Fresh Foods①Comparison of Survey Results and Analytical Methods].","authors":"Yu Shiozawa, Rio Suzuki, Shui Watanabe, Ayako Ito, Rina Nakamura, Toshiko Shimoi, Yukiko Yamajima, Narue Sakamaki, Kenji Otsuka, Maki Kanda","doi":"10.3358/shokueishi.67.67","DOIUrl":"https://doi.org/10.3358/shokueishi.67.67","url":null,"abstract":"Benzoic acid (BA),widely used as a food preservative, occurs naturally in various natural foods. Therefore, distinguishing between added and naturally occurring BA is essential in the administrative testing of food preservatives. To elucidate the naturally derived BA levels, we analyzed 180 samples, including fruits, nuts, vegetables, beans, and mushrooms, using steam distillation and dialysis. The distillation method successfully detected BA in 60 samples, with concentrations ranging from 2.3 to 471.2 μg/g. Particularly high levels were detected in cranberry and red perilla. In contrast, the dialysis method could not detect BA in 21 fruit samples with low BA concentrations and detected only trace amounts of BA in four samples. These findings indicated that BA might be generated in fruit samples during the distillation process. This study provides valuable reference data for administrative assessments when determining whether BA detected in food is naturally derived or added as a preservative.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"67 2","pages":"67-73"},"PeriodicalIF":0.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148320654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Rapid Bacterial Testing for Food Safety: A Case Study from the Tokyo 2020 Games]. [食品安全快速细菌检测：以2020年东京奥运会为例]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.114

Makiko Kobayashi, Miki Ida, Chikako Asayama, Rei Kato, Yoko Higuchi, Dai Saiki, Toshiyuki Kinugasa, Motomi Takaku, Hirofumi Miyake, Keiko Yokoyama, Kenji Sadamasu

{"title":"[Rapid Bacterial Testing for Food Safety: A Case Study from the Tokyo 2020 Games].","authors":"Makiko Kobayashi, Miki Ida, Chikako Asayama, Rei Kato, Yoko Higuchi, Dai Saiki, Toshiyuki Kinugasa, Motomi Takaku, Hirofumi Miyake, Keiko Yokoyama, Kenji Sadamasu","doi":"10.3358/shokueishi.67.114","DOIUrl":"https://doi.org/10.3358/shokueishi.67.114","url":null,"abstract":"This study investigated the effectiveness of an automated enumeration system for viable bacteria (TEMPO method) in comparison with conventional methods. Subsequently, the method was applied to detect bacterial contamination in food and other samples to ensure food hygiene and safety during the Tokyo 2020 Olympic and Paralympic Games (Tokyo 2020 Games).Comparative testing using commercially available food products demonstrated consistent results across most test items, however, the results were discrepant for vegetables and fruits (agreement rate: 71-88%).During the Tokyo 2020 Games, which were conducted as a large-scale international event, using the TEMPO method considerably decreased the workload by eliminating tasks such as media preparation, serial dilution, sample-agar mixture preparation, bacterial count measurement, and follow-up evaluation of positive samples. Consequently, the TEMPO method substantially decreased the testing time and the number of required personnel. Therefore, rapid bacterial testing approach is an effective method to support and validate food hygiene and safety in such large-scale events.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"67 3","pages":"114-119"},"PeriodicalIF":0.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148413772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Association between Processing Conditions and Microbial Detection in Game Meat]. [野味加工条件与微生物检测的关系]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.161

Hirosato Katoh, Hinata Tasaki, Yuya Tagawa, Ryusei Fujiuchi, Mizuka Nagata, Tomoaki Imamura

{"title":"[Association between Processing Conditions and Microbial Detection in Game Meat].","authors":"Hirosato Katoh, Hinata Tasaki, Yuya Tagawa, Ryusei Fujiuchi, Mizuka Nagata, Tomoaki Imamura","doi":"10.3358/shokueishi.67.161","DOIUrl":"https://doi.org/10.3358/shokueishi.67.161","url":null,"abstract":"This study compared microbial detection among game meat samples processed under different conditions. Game animals captured in Oita and Osaka Prefectures between November 2019 and March 2023 were classified into three groups according to processing conditions: on-site processing (n=8), field-based processing (n=30), and licensed facility processing group (n=223). Swab samples were collected from the abdominal cavity and thigh muscle, and aerobic bacteria, coliform bacteria, and staphylococci were examined using commercially available stamp agar plates. Detection rates were compared using Fisher's exact test.Bacteria were detected in all individuals processed under outdoor conditions (on-site and field-base groups), whereas no bacteria were detected in the licensed facility processing group (0/223)(p<0.001). No significant differences were observed between the two outdoor processing groups.These findings indicate that the on-site and field-based processing groups showed higher detection rates of indicator bacteria than the licensed facility processing group. However, this was an observational study, and the findings do not directly demonstrate a causal effect of processing conditions. The results may also have been affected by regional differences, capture methods, seasonal differences, limited sample size in the on-site group, and pre-selection before facility entry.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"67 4","pages":"161-164"},"PeriodicalIF":0.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148882186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Improvement of the Material Test Method for Diphenyl Carbonate and Bisphenol A in Polycarbonate Food Apparatuses, Containers, and Packaging]. [聚碳酸酯食品器具、容器和包装中碳酸二苯酯和双酚A材料试验方法的改进]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.181

Yohei Kataoka, Motoh Mutsuga, Yutaka Abe, Midori Kondo, Kyoko Sato, Naoki Sugimoto

引用次数: 0

[Migration of Primarily Metallic Elements from Commercial Paper and Bamboo Straws]. [商业纸和竹吸管中主要金属元素的迁移]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.53

Koji Fujihara, Midori Kondo, Eri Kishi, Chiharu Mizuguchi-Fukase, Asako Ozaki, Yohei Kataoka, Naoki Sugimoto, Motoh Mutsuga

{"title":"[Migration of Primarily Metallic Elements from Commercial Paper and Bamboo Straws].","authors":"Koji Fujihara, Midori Kondo, Eri Kishi, Chiharu Mizuguchi-Fukase, Asako Ozaki, Yohei Kataoka, Naoki Sugimoto, Motoh Mutsuga","doi":"10.3358/shokueishi.67.53","DOIUrl":"https://doi.org/10.3358/shokueishi.67.53","url":null,"abstract":"The amount of primarily metallic elements that migrated from commercial paper and bamboo straws was measured using 4% acetic acid and water as food simulants for 30 minutes at room temperature and the results were compared with those from products provided by the Japan Paper Association and synthetic resin straws. Additionally, the content in paper and bamboo straws was analyzed, and human exposure to elements from these straws was estimated. Results showed that Al, Mn, and Zn migrated from nearly all commercial straw products, while Ni, Cu, As, and Pb migrated from a few commercial paper and bamboo straw products during extraction using 4% acetic acid. In contrast, only Al and Zn migrated from synthetic resin straws. Paper straws also showed that elements were more likely to migrate into acidic beverages. Notably, As, Cd, Sn, and Pb were detected in most commercial straws, though none of those elements migrated from most commercial products and all provided products. Therefore, it is considered necessary to pay attention to the conditions of use, such as frequency of use, when paper and bamboo straws are used with acidic beverages.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"67 2","pages":"53-60"},"PeriodicalIF":0.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148320537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Simple Omphalotus japonicus Identification Method for General Use]. [通用简法日本参鉴定方法]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.85

Hideyuki Shinohara, Satomu Ito, Yoko Aoki

引用次数: 0

[Study of Rapid Analytical Method for Fumigants in Powdered Infant Formulas by LC-MS/MS]. [LC-MS/MS快速分析婴幼儿配方奶粉中熏蒸剂的方法研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.40

Takumi Daimon, Keiko Oguchi

引用次数: 0

[Preparation of Incurred Samples Containing Egg-derived Protein and the Pilot Study for Proficiency Test]. [含蛋源性蛋白的制备及能力测试的初步研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2026-01-01 DOI: 10.3358/shokueishi.67.16

Mai Hosoya, Kana Uchida, Shinobu Wakuri, Shihomi Kuwano, Rie Ito, Yusuke Iwasaki, Takaho Watanabe, Hiroshi Akiyama

{"title":"[Preparation of Incurred Samples Containing Egg-derived Protein and the Pilot Study for Proficiency Test].","authors":"Mai Hosoya, Kana Uchida, Shinobu Wakuri, Shihomi Kuwano, Rie Ito, Yusuke Iwasaki, Takaho Watanabe, Hiroshi Akiyama","doi":"10.3358/shokueishi.67.16","DOIUrl":"https://doi.org/10.3358/shokueishi.67.16","url":null,"abstract":"In allergen testing of food products, external quality assessment (EQA) programs are essential to ensure high-precision detection and the reliability of results across testing laboratories. However, conventional spiked samples may not sufficiently reflect the nature of processed foods. Therefore, this study examined the applicability of EQA programs using actual food samples that reflect real-world conditions. Twenty-four commercially available food samples were screened using the ELISA method. A coleslaw dressing (CoD) containing 33.1 μg/g of egg-derived protein which is closest to the labeling reference value of 10 μg/g, was selected. The CoD was diluted with egg-free CoD, resulting in a mean concentration of 10.4±0.6 μg/g (RSD 5.3%).Based on this result, approximately 2 kg of an incurred sample (Sample 1) was prepared for a sample in the pilot study for the EQA program, targeting a concentration of 10 μg/g, and its homogeneity and stability were confirmed. A conventional spiked sample (Sample 2) was also prepared with equivalent quality. A pilot study for the EQA program was conducted with 22 institutions. By using two kinds of ELISA kits, the average concentration was 11.9 μg and 9.7 μg for Sample 1 and 8.6 μg/g and 9.3 μg/g for Sample 2. The performance evaluation using robust statistical methods indicated that nearly all measurement results from the institutions fell within the control limits, suggesting that the incurred sample is beneficial as a test material for the EQA program. These results suggest that EQA programs using incurred samples can contribute to improving the reliability of egg allergen testing in food safety management.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"67 1","pages":"16-21"},"PeriodicalIF":0.2,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147312559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0