Food Hygiene and Safety Science最新文献_第10页

[Determination of Albendazole Metabolite in Livestock Products Using LC-MS/MS]. 畜产品中阿苯达唑代谢物的LC-MS/MS测定

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.113

Ryo Okabe, Akiko Kubota, Satoru Nemoto, Mitsutoshi Aoyagi

引用次数: 0

[Detection of Ciguatoxins from Fish Introduced into a Wholesale Market in Japan]. [从日本批发市场进口的鱼类中检测出雪卡毒素]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.8

Naomasa Oshiro, Takumi Tomikawa, Kyoko Kuniyoshi, Keisuke Kimura, Takashi Kojima, Takeshi Yasumoto, Hiroshi Asakura

{"title":"[Detection of Ciguatoxins from Fish Introduced into a Wholesale Market in Japan].","authors":"Naomasa Oshiro, Takumi Tomikawa, Kyoko Kuniyoshi, Keisuke Kimura, Takashi Kojima, Takeshi Yasumoto, Hiroshi Asakura","doi":"10.3358/shokueishi.62.8","DOIUrl":"https://doi.org/10.3358/shokueishi.62.8","url":null,"abstract":"Ciguatera fish poisoning (CFP), one of the most frequently occurring seafood poisonings due to marine finfish consumption, mainly affects the tropical and subtropical Indo-Pacific region and the Caribbean Sea. The principal class of toxins, ciguatoxins (CTXs) from the Pacific, includes more than 20 derivatives and are classified into two groups, CTX1B and CTX3C congeners, based on their skeletal structures. As part of risk management of CFP by the Japanese government, the import of certain species of fish into Japan is prohibited. Additionally, local governments recommend rejecting certain fish species caught in Japan. In this study, we used LC-MS/MS to analyze CTXs from 18 fish specimens belonging to 7 species that had been brought to a wholesale market but were disapproved for sale because of their potential danger of CFP. CTXs were detected in four specimens of Lutjanus bohar and one specimen of Variola louti. It was estimated that the two most poisonous specimens (no. 5: 0.348 μg/kg, no. 8: 0.362 μg/kg) had a toxicity of 0.05 MU/g. Consumption of 200 g of flesh from these fish could cause CFP. Thus, the guidance of the local government to disallow the sale of these fish species in the market contributed to the prevention of CFP.Only CTX1B congeners were detected in L. bohar (specimen no. 5), which had no record of the area where it captured from. It is presumed that the origin of specimen no. 5 was the same as that of the Okinawan L. bohar because the CTX compositions were similar. In two specimens (nos. 6 and 8) from Wakayama, both CTX1B and CTX3C congeners were detected. This is the first report to reveal the CTX profile in fish collected off the Honshu island in Japan.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 1","pages":"8-13"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25433823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

[Evaluation of a Risk Communication Program for Pesticide Residues]. [农药残留风险沟通项目的评估]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.187

Hiroshi Akiyama, Aya Takagi, Koichi Inoue, Yoshinari Suzuki, Rie Ito, Nobuyuki Wakui, Mayumi Asai, Junkichi Sugiura

{"title":"[Evaluation of a Risk Communication Program for Pesticide Residues].","authors":"Hiroshi Akiyama, Aya Takagi, Koichi Inoue, Yoshinari Suzuki, Rie Ito, Nobuyuki Wakui, Mayumi Asai, Junkichi Sugiura","doi":"10.3358/shokueishi.62.187","DOIUrl":"https://doi.org/10.3358/shokueishi.62.187","url":null,"abstract":"To promote and raise the awareness of accurate knowledge on pesticide residues, the symposium program on risk communication on pesticide residues was held by the broadcasted online style. The risk communication program was statistically evaluated using a pre- and post-program online questionnaire survey. We had the questionnaire answers of the 105 valid participants. The analysis of post-program questionnaires shows that the risk communication program was effective in terms of levels of understanding and interest. Pre-program risk perception or awareness of safety assessments was significantly and positively correlated with awareness for establishing standard values of pesticide residues by the analysis of pre-program questionnaires. Risk perception after the program was significantly higher than before the program, suggesting that risk perception increased due to the program by analysis the same question between pre- and post-program questionnaires. Multiple regression analysis suggests that the participants with higher pre-program awareness of safety assessments or pre-program awareness for establishing standard values appeared to have higher levels of understanding and post-program risk perception.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 6","pages":"187-192"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39851733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of Cooking and Processing on Quantitation of Soybean Proteins]. [烹饪和加工对大豆蛋白定量的影响]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.193

Hiroko Watanabe, Haruyo Akiyama, Nobuhiko Osawa, Kaori Imura, Naomi Iseki, Sumiko Ueda, Tomoka Masaoka, Chie Akaboshi

{"title":"[Effect of Cooking and Processing on Quantitation of Soybean Proteins].","authors":"Hiroko Watanabe, Haruyo Akiyama, Nobuhiko Osawa, Kaori Imura, Naomi Iseki, Sumiko Ueda, Tomoka Masaoka, Chie Akaboshi","doi":"10.3358/shokueishi.62.193","DOIUrl":"10.3358/shokueishi.62.193","url":null,"abstract":"We examined the effects of cooking and processing on the quantitation of soy protein in various soy-based foods. For the phosphate-buffered saline (PBS) extraction, the total protein content was measured by bicinchoninic acid assay, and the buffer extraction containing sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (ME) was measured by the 2-D Quant Kit, and SDS-polyacrylamide gel electrophoresis analysis (SDS-PAGE) of each extraction was performed. Furthermore, measurements were performed by various ELISAs. During the tofu cooking process, the protein concentrations of soaked soybeans and Seigo (soybean homogenized with water) fluctuated- the change in protein solubility due to the amount of water during sample homogenization was considered to be a factor. It was thought that the decrease in protein concentration due to heating of Seigo during soymilk production was considered to indicate thermal denaturation of the protein, and that SDS and ME extraction in tofu may affect the measurement system. In cooking excluding roasted beans, proteins with a mass of 50 kDa or above and around 20 kDa were denatured, and in twice-fried tofu, proteins around 40 kDa were denatured, but the protein concentration excluding boiled soybeans did not decreased. Furthermore, the protein concentration from roasted beans, yuba, roasted okara and fried tofu increased with the cooking time, suggesting that the denaturation temperature of the protein shifted to a high temperature as the water content decreased. Both of the two types of ELISAs that comply with the official labeling system of foods containing allergenic substances were useful for detecting soybean protein by detecting proteins and peptides in processed soybean products, fermented foods excluding natto, and health foods.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 6","pages":"193-202"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39851734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

[Effect of Soy Sauce, Fish Sauce for Nitrite Determination in Meat Products]. 酱油、鱼露对肉制品中亚硝酸盐测定的影响

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.105

Ayako Matsuzaka, Akio Ito, Yurika Awano, Makiko Tonohara, Kaori Yokomizo

引用次数: 0

[Determination of Caffeine, Theobromine, and Theophylline in Chocolate Using LC-MS]. 液相色谱-质谱法测定巧克力中的咖啡因、可可碱和茶碱

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.119

Tomoya Tanaka, Keisuke Kimura, Kimiko Kan, Tetsuya Shindo, Takeo Sasamoto

引用次数: 0

[Rapid Determination of tert-Butylhydroquinone (TBHQ) in Foods Using Three-Layer Extraction]. [三层萃取法快速测定食品中叔丁基对苯二酚[j]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.125

Takumi Daimon, Kunihiko Akahashi

引用次数: 1

[Usefulness of Dilution for Rapid Simultaneous Determination of Residual Pesticides in Agricultural Products by LC-MS/MS]. [稀释法在LC-MS/MS快速同时测定农产品中残留农药中的应用]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.166

Takumi Daimon, Aya Tamura, Kunihiko Takahashi

引用次数: 0

[Rapid Screening System for Paralytic Shellfish Toxins in Bivalves by Oligonucleotide Lateral Flow Immunoassay]. [利用寡核苷酸侧流免疫分析法快速筛选双壳类动物麻痹性贝类毒素]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.85

Ken-Ichi Ueno, Aoi Hosokawa, Satoshi Hashimoto, Hiroshi Oikawa, Yusuke Shibahara, Ryoji Matsushima, Ryuichi Watanabe, Hajime Uchida, Toshiyuki Suzuki

{"title":"[Rapid Screening System for Paralytic Shellfish Toxins in Bivalves by Oligonucleotide Lateral Flow Immunoassay].","authors":"Ken-Ichi Ueno, Aoi Hosokawa, Satoshi Hashimoto, Hiroshi Oikawa, Yusuke Shibahara, Ryoji Matsushima, Ryuichi Watanabe, Hajime Uchida, Toshiyuki Suzuki","doi":"10.3358/shokueishi.62.85","DOIUrl":"https://doi.org/10.3358/shokueishi.62.85","url":null,"abstract":"The mouse bioassay (MBA) for paralytic shellfish toxins (PSTs) in bivalves has been used as an official method in Japan. It is necessary to develop an alternative method to animal experiments in PSTs assay because 3Rs (Replacement, Reduction, and Refinement) of animal experiments are required from the animal welfare point of view. Various methods such as HPLC-FL, receptor binding assay, LC-MS/MS and ELISA have been established to detect PSTs without performing animal experiments. The present study was undertaken to develop a screening method using oligonucleotide lateral flow immunoassay (OLFIA) for detecting PSTs in bivalves. The screening level was defined as positive at 2 MU/g of MBA that is the half regulation limit of PSTs monitoring in Japan. All 20 positive (equal to or more than 2 MU/g) samples judged from MBA showed a positive reaction in the OLFIA. No positive samples resulted in a false negative reaction. The OLFIA exhibited high accuracy at 2 MU/g of screening criteria. The authors demonstrated here that the OLFIA can be useful for rapid detection of PSTs in bivalves.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 3","pages":"85-93"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

[In vivo and In vitro Mitigation Effects of Lactic Acid Bacteria Derived from Fresh Vegetables on Aflatoxins]. [新鲜蔬菜乳酸菌对黄曲霉毒素的体内外抑制作用]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2021-01-01 DOI: 10.3358/shokueishi.62.148

Kenji Oonaka, Naoki Kobayashi, Yosuke Uchiyama, Mioko Honda, Shiro Miyake, Yoshiko Sugita-Konishi

{"title":"[In vivo and In vitro Mitigation Effects of Lactic Acid Bacteria Derived from Fresh Vegetables on Aflatoxins].","authors":"Kenji Oonaka, Naoki Kobayashi, Yosuke Uchiyama, Mioko Honda, Shiro Miyake, Yoshiko Sugita-Konishi","doi":"10.3358/shokueishi.62.148","DOIUrl":"https://doi.org/10.3358/shokueishi.62.148","url":null,"abstract":"Aflatoxins (AFs) are known to be oncogenic mycotoxins. This study investigated the mitigation effects of lactic acid bacteria (LAB) isolated from four types of vegetable, cucumber, Chinese cabbage, Japanese radish and eggplant, which are used to make Japanese traditional fermented pickles, on AFs. Using aflatoxin M1 (AFM1) binding assay for screening, four representative strains were selected (one from each vegetable) from total 94 LAB strains, based on the highest binding ratio. The ranges of the binding ratio of these representative strains to aflatoxin B1 (AFB1), aflatoxin B2, aflatoxin G1, aflatoxin G2 and AFM1 were 57.5%-87.9% for the LAB strain derived from cucumber, 18.9%-43.9% for the LAB strain derived from Chinese cabbage, 26.4%-41.7% for the LAB strain derived from Japanese radish, and 15.0%-42.6% for the LAB strain derived from eggplant. The strains isolated from cucumber, Chinese cabbage, Japanese radish and eggplant were identified as Lactococcus lactis subsp. lactis, Weissella cibaria, Leuconostoc mesenteroides and Leu. mesenteroides, respectively. An in vitro binding assay of the four strains under acidic conditions showed that the number of living bacteria decreased, while the binding ratio increased in some strains, suggesting that the LAB maintained their capacity to bind aflatoxins even in an environment that imitated the stomach. An in vivo experiment using L. lactis subsp. lactis derived from cucumber revealed that the bacteria significantly inhibited the absorption of AFB1 into blood. These results showed that the LAB used for Japanese vegetable pickles was an effective binding agent of AFs and suggested that they might play a role in mitigating AF absorption.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"62 5","pages":"148-156"},"PeriodicalIF":0.3,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39588258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0