Food Hygiene and Safety Science最新文献_第2页

[Study on Weight Change Rate after Rehydration of Dried Kotake Mushroom]. [干菇复水后重量变化率的研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.167

Hiromi Nabeshi, Tomomi Maeda, Atsuko Ikarashi, Kayo Kawamata, Tomoaki Tsutsumi

{"title":"[Study on Weight Change Rate after Rehydration of Dried Kotake Mushroom].","authors":"Hiromi Nabeshi, Tomomi Maeda, Atsuko Ikarashi, Kayo Kawamata, Tomoaki Tsutsumi","doi":"10.3358/shokueishi.65.167","DOIUrl":"10.3358/shokueishi.65.167","url":null,"abstract":"The level of radioactive cesium in food that is generally consumed in the rehydrated state can be calculated from measurements taken in the dried state using the specific weight change rate set by the Ministry of Health, Labour and Welfare. However, only a few dried foods have a specified weight change rate. Accurate specific weight change rates are critical in determining the compliance of a dried food item with Japanese maximum limits (JMLs) for radioactivity. We investigated the weight change rate of dried to rehydrated kotake mushrooms, which may contain relatively high concentrations of radioactive cesium and whose weight change rate has a large effect on the judgment of JML compliance. The average weight change rate of dried kotake mushrooms was 5.7 (range: 4.2 to 6.9), which was 1.4 times larger than the currently applied weight change rate of 4.0. Since the minimum weight change rate was 4.2 in this study, it was considered that the weight change rate currently applied to dried kotake mushrooms was a reasonable value. Further data is required in order to set specified weight change rates and to evaluate the validity of currently applied weight change rates in dried kotake mushroom.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"167-171"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Quantitative Analysis and Characteristics of Salmonella from Retail Chicken Meat Products in Japan]. [日本零售鸡肉产品中沙门氏菌的定量分析及特征]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.101

Shunsuke Ikeuchi, Yoshimasa Sasaki, Minato Okumura, Takeshi Niwa, Yukiko Hara-Kudo, Hideki Hayashidani

{"title":"[Quantitative Analysis and Characteristics of Salmonella from Retail Chicken Meat Products in Japan].","authors":"Shunsuke Ikeuchi, Yoshimasa Sasaki, Minato Okumura, Takeshi Niwa, Yukiko Hara-Kudo, Hideki Hayashidani","doi":"10.3358/shokueishi.65.101","DOIUrl":"10.3358/shokueishi.65.101","url":null,"abstract":"From October 2020 to February 2021, a total of 95 retail chicken meat products from 39 retail meat shops in Tokyo Metropolis and Kanagawa Prefecture, Japan, were collected and examined for the prevalence of Salmonella to assess public health implications. If a sample tested positive for Salmonella, a quantitative analysis was performed using the three-tube most probable number (MPN) method. Of 95 retail chicken meat products, Salmonella was isolated from 30 samples (31.6%). The levels of Salmonella contamination ranged from <0.3 to 4.3 MPN/g. The most frequent level was <0.3 MPN/g (63.3%). Of the 33 Salmonella strains isolated, four serotypes were identified: S. Schwarzengrund (60.6%), S. Infantis (24.2%), S. Agona (12.1%), and S. Manhattan (3.0%). Multilocus sequence typing (MLST) analysis classified most S. Schwarzengrund isolates into sequence type (ST) 241, the same ST found in chicken meat in Japan, except for one isolate. Of the 33 Salmonella isolates, 29 (87.9%) were antibiotic resistant. Twenty-six isolates (78.8%) showed multidrug resistance to two or more antibiotics. Therefore, these results indicate that retail chicken meat products in Japan are an important source of Salmonella infection in humans and that Salmonella contamination in retail chicken products seems to originate from chicken meat.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"101-106"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Validation Study of LC-MS/MS for Paralytic Shellfish Toxins and Tetrodotoxin in Scallops]. [LC-MS/MS检测扇贝中麻痹性贝类毒素和河豚毒素的验证研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.129

Satoshi Numano, Ryuichi Watanabe, Mayu Ozawa, Hajime Uchida, Ryoji Matsushima, Toshiyuki Suzuki

{"title":"[Validation Study of LC-MS/MS for Paralytic Shellfish Toxins and Tetrodotoxin in Scallops].","authors":"Satoshi Numano, Ryuichi Watanabe, Mayu Ozawa, Hajime Uchida, Ryoji Matsushima, Toshiyuki Suzuki","doi":"10.3358/shokueishi.65.129","DOIUrl":"https://doi.org/10.3358/shokueishi.65.129","url":null,"abstract":"Recently, an instrumental analysis using LC-MS/MS has been developed and validated for paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) in bivalve molluscs in Japanese domestic and overseas. The method for 11 PSTs and TTX in scallops was validated in accordance with a previous report and CODEX-STAN. The samples were prepared by adding the standard mixture of PSTs and TTX to scallop (Patinopecten yessoensis) homogenates, extracted with 1% acetic acid and then cleaned up using an ENVI-Carb (250 mg/3 mL) cartridge. A single analyst extracted and analyzed in two samples per day on five successive days. As result of the validation, 11 PSTs were found falling within all the guideline criteria (the trueness, repeatability, within laboratory reproducibility, LOD, LOQ) in the tests using matrix-matched calibration or solvent calibration.However, the trueness of TTX was low value in the test using solvent standard. It would be due to the matrix effects at the LC-MS/MS analysis. The slope of the calibration curve at the matrix-matched standards of mussel was about the same as the slope at the solvent standards. Therefore, it is necessary to choose the most appropriate matrix depending on shellfish.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"129-135"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Implementation and Evaluation of Risk Communication Regarding Residual Pesticides]. [残留农药风险交流的实施与评估]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.20

Nobuyuki Wakui, Koichi Inoue, Mari Nunome, Yoshinari Suzuki, Aya Takagi, Rie Ito, Yusuke Iwasaki, Junkichi Sugiura, Hiroshi Akiyama

{"title":"[Implementation and Evaluation of Risk Communication Regarding Residual Pesticides].","authors":"Nobuyuki Wakui, Koichi Inoue, Mari Nunome, Yoshinari Suzuki, Aya Takagi, Rie Ito, Yusuke Iwasaki, Junkichi Sugiura, Hiroshi Akiyama","doi":"10.3358/shokueishi.65.20","DOIUrl":"10.3358/shokueishi.65.20","url":null,"abstract":"In this study, a public seminar on risk communication methods was conducted to raise awareness and disseminate accurate knowledge about residual pesticides to consumers. Additionally, surveys on consumer awareness were conducted on the attendees before and after the seminar to evaluate its effectiveness. Responses were obtained from 84 participants. The paired t-test was used to analyze the changes in awareness before and after the seminar. The results showed significant improvements in \"trust in the government\" and \"understanding of residual pesticides.\" Furthermore, step-wise multiple regression analysis was performed to explore the factors influencing satisfaction with the risk communication seminar, and the item \"understanding of the safety of residual pesticides in food\" was extracted. Understanding food safety is a crucial concern in daily life for consumers. To enable consumers to have an accurate understanding of food risks and make appropriate judgments, it is essential to continue implementing risk communication and conveying information about food safety and security in the future.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 1","pages":"20-23"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Universal Method for Determination of Ciguatoxins by LC-MS/MS]. [采用 LC-MS/MS 方法测定雪茄毒素的通用方法]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.72

Kyohei Yamada, Hiroshi Shitara, Noriko Kanda, Takahide Kondo, Michihiro Nishida, Naomasa Oshiro

{"title":"[Universal Method for Determination of Ciguatoxins by LC-MS/MS].","authors":"Kyohei Yamada, Hiroshi Shitara, Noriko Kanda, Takahide Kondo, Michihiro Nishida, Naomasa Oshiro","doi":"10.3358/shokueishi.65.72","DOIUrl":"10.3358/shokueishi.65.72","url":null,"abstract":"Ciguatera poisoning (CP) is one of the most frequent seafood poisonings across the globe. CP results from the consumption of fish flesh that has accumulated principal toxins known as ciguatoxins (CTXs), and it mainly occurs in tropical and subtropical regions. In Japan, incidents of CP have been reported primarily from Okinawa and Amami Islands in the subtropical area. Meanwhile, there have also been reports from Mainland sporadically. Since the amount of CTXs contained in fish flesh is extremely low, a highly sensitive detection method by LC-MS/MS is required. But the currently reported detection method is applicable only to specific equipment, and many laboratories have difficulty to respond CP. In this study, to prepare for the risk of nationwide CP, we researched a universal analytical method for CTXs based on LC-MS/MS. Using a water/acetonitrile mobile phase supplemented with lithium hydroxide and formic acid gave rise to prominent peaks of the stable [M+Li]+ions. As the [M+Li]+ions did not produce valid product ions even with high collision energy, the [M+Li]+ions of each analog were set for both precursor and product ions ([M+Li]+>[M+Li]+) and monitored under the multiple reaction monitoring (MRM) mode. With the method described above, analyses of nine CTX congeners were carried out. The limit of detection (LOD, S/N>5) and quantitation (LOQ, S/N>10) were estimated as 0.005-0.030 ng/mL and 0.010-0.061 ng/mL, respectively. When the 1 mL of extract solution is prepared from 5 g of the fish tissue, the LOD and LOQ will be at 0.001-0.006 μg/kg and 0.002-0.012 μg/kg, respectively. This result indicates that we could detect the required level of 0.175 μg/kg CTX1B equivalent in fish flesh which is recommended for safe consumption in Japan. This method is considered to be a universal analytical method without depending on the specific equipment. Thus it could contribute to improving the CP investigations in nationwide laboratories.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 3","pages":"72-77"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Laboratory Performance Study of the Japanese Official Method to Detect Genetically Modified Papaya Line PRSV-YK]. [检测转基因木瓜品系 PRSV-YK 的日本官方方法的实验室性能研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.61

Norihito Shibata, Toshiaki Nakasaka, Jumpei Narushima, Chie Taguchi, Miyu Sugino, Satoko Yoshiba, Keisuke Soga, Michika Kajiwara, Takaho Watanabe, Kazunari Kondo

{"title":"[Laboratory Performance Study of the Japanese Official Method to Detect Genetically Modified Papaya Line PRSV-YK].","authors":"Norihito Shibata, Toshiaki Nakasaka, Jumpei Narushima, Chie Taguchi, Miyu Sugino, Satoko Yoshiba, Keisuke Soga, Michika Kajiwara, Takaho Watanabe, Kazunari Kondo","doi":"10.3358/shokueishi.65.61","DOIUrl":"10.3358/shokueishi.65.61","url":null,"abstract":"Since the establishment of procedures for the safety assessment of food products that use recombinant DNA technology, the manufacture, import, and sale of genetically modified (GM) foods that have not undergone safety assessment are prohibited under the Food Sanitation Act. Therefore, a performance study to confirm the GM food testing operations of each laboratory is very important to ensure the reliability of the GM food monitoring system. In 2022, GM papaya line PRSV-YK-which has not yet been authorized in Japan-was selected for testing, and a papaya paste and a DNA solution were used as the test samples. With these samples, a laboratory performance study of the DNA extraction and real-time PCR operations was conducted. This confirmed that the 18 participating laboratories were generally performing the DNA extraction and real-time PCR operations correctly. However, some laboratories using certain DNA amplification reagent with some real-time PCR instruments were not able to determine the PRSV-YK detection test. This suggests that the PRSV-YK detection test may not be able to correctly detect samples containing GM papaya when performed with these combinations of instruments and reagent. In order to ensure the reliability of the PRSV-YK detection test, it is necessary to examine in detail how the combination of DNA polymerase reagents and real-time PCR instruments affects the detection limit, and to implement an appropriate solution.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 3","pages":"61-66"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Application of High-performance Liquid Chromatography to Caprolactam Migration Testing of Food Utensils, Containers, and Packaging]. 【高效液相色谱法在食品器具、容器和包装中己内酰胺迁移检测中的应用】

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.107

Yutaka Abe, Miku Yamaguchi, Koji Fujihara, Yohei Kataoka, Motoh Mutsuga, Naoki Sugimoto

引用次数: 0

[Single-Laboratory Validation Study of Simultaneous Determination Methodfor Five Organochlorine Unstable Pesticides in Agricultural Products by GC-MS/MS]. [GC-MS/MS同时测定农产品中5种有机氯不稳定农药方法的单实验室验证研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.124

Yoshihiro Ohsawa, Sanae Tomizawa, Kyoko Kamijo, Takayuki Nakajima, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama

{"title":"[Single-Laboratory Validation Study of Simultaneous Determination Methodfor Five Organochlorine Unstable Pesticides in Agricultural Products by GC-MS/MS].","authors":"Yoshihiro Ohsawa, Sanae Tomizawa, Kyoko Kamijo, Takayuki Nakajima, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama","doi":"10.3358/shokueishi.65.124","DOIUrl":"https://doi.org/10.3358/shokueishi.65.124","url":null,"abstract":"A method was developed for determining five organochlorine unstable pesticides (captan, chlorothalonil, dichlofluanid, folpet, and tolylfluanid) in agricultural products using GC-MS/MS. To prevent pesticide degradation, the food sample was maintained at -20℃ until the initiation of the extraction process. The sample underwent homogenization with acetonitrile and salting out using anhydrous magnesium sulfate and sodium chloride in the presence of citrate salts. Cleanup was executed using Graphite Carbon and C18 SPE cartridges. The validation of this method was tested on five agricultural products at two concentrations (0.01 and 0.1 μg/g). The recovery rates varied between 86.7% and 96.1%. RSDs for repeatability were less than 15.2% and 8.8%, while the RSDs for within-laboratory reproducibility were under 19.9% and 12.7%. These results meet the criteria established by the validation guidelines in Japan.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"124-128"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development of a Rapid Analytical Method for Simultaneous Determinationof Diverse Plant and Mushroom Toxins]. [同时测定多种植物和蘑菇毒素的快速分析方法的建立]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.172

Toru Fukumitsu, Masahito Hagio, Kenichi Kumasaka

引用次数: 0

[Stochastic Considerations on the Upper and Lower Limits of Colony Counts on Agar Plate for Microbial Count Calculation]. 【计算微生物数量时琼脂平板菌落数量上下限的随机考虑】。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.113

Hiroshi Fujikawa

{"title":"[Stochastic Considerations on the Upper and Lower Limits of Colony Counts on Agar Plate for Microbial Count Calculation].","authors":"Hiroshi Fujikawa","doi":"10.3358/shokueishi.65.113","DOIUrl":"https://doi.org/10.3358/shokueishi.65.113","url":null,"abstract":"The upper and lower limits of microbial colony count on agar plate are essential for microbial cell calculation of food samples. In the present study, the limits presented by the three standards of Ministry of Health, Labor, and Welfare of Japan, Food and Drug Administration of US (FDA), and Japanese Industrial Standards (JIS) were studied from the stochastic point of view. Here colony counts per plate were assumed to follow the Poisson distribution from our previous studies. The probability of acceptance of a pair of colony counts, PA for cell calculation by each standard was then calculated for various values of the mean of the Poisson distribution theoretically and by simulation with random sampling numbers. The values of PA at the lower and upper limits of the standards were low. For example, PA at the mean of 25 which is the lower limit of the FDA standard was 0.278. The values of PA of colony count pairs by the three standards were demonstrated in a wide range of the mean colony count. The largest range of the mean at PA>0.5 was observed in the JIS standard among the three. A new method to determine the limits for colony counts was developed with the risk of not acceptance. These findings would be helpful to consider the limits of colony counts for cell calculation.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"113-117"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0