Food Hygiene and Safety Science最新文献_第2页

[Application of High-performance Liquid Chromatography to Caprolactam Migration Testing of Food Utensils, Containers, and Packaging]. 【高效液相色谱法在食品器具、容器和包装中己内酰胺迁移检测中的应用】

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.107

Yutaka Abe, Miku Yamaguchi, Koji Fujihara, Yohei Kataoka, Motoh Mutsuga, Naoki Sugimoto

引用次数: 0

[Single-Laboratory Validation Study of Simultaneous Determination Methodfor Five Organochlorine Unstable Pesticides in Agricultural Products by GC-MS/MS]. [GC-MS/MS同时测定农产品中5种有机氯不稳定农药方法的单实验室验证研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.124

Yoshihiro Ohsawa, Sanae Tomizawa, Kyoko Kamijo, Takayuki Nakajima, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama

{"title":"[Single-Laboratory Validation Study of Simultaneous Determination Methodfor Five Organochlorine Unstable Pesticides in Agricultural Products by GC-MS/MS].","authors":"Yoshihiro Ohsawa, Sanae Tomizawa, Kyoko Kamijo, Takayuki Nakajima, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama","doi":"10.3358/shokueishi.65.124","DOIUrl":"https://doi.org/10.3358/shokueishi.65.124","url":null,"abstract":"A method was developed for determining five organochlorine unstable pesticides (captan, chlorothalonil, dichlofluanid, folpet, and tolylfluanid) in agricultural products using GC-MS/MS. To prevent pesticide degradation, the food sample was maintained at -20℃ until the initiation of the extraction process. The sample underwent homogenization with acetonitrile and salting out using anhydrous magnesium sulfate and sodium chloride in the presence of citrate salts. Cleanup was executed using Graphite Carbon and C18 SPE cartridges. The validation of this method was tested on five agricultural products at two concentrations (0.01 and 0.1 μg/g). The recovery rates varied between 86.7% and 96.1%. RSDs for repeatability were less than 15.2% and 8.8%, while the RSDs for within-laboratory reproducibility were under 19.9% and 12.7%. These results meet the criteria established by the validation guidelines in Japan.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"124-128"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development of a Rapid Analytical Method for Simultaneous Determinationof Diverse Plant and Mushroom Toxins]. [同时测定多种植物和蘑菇毒素的快速分析方法的建立]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.172

Toru Fukumitsu, Masahito Hagio, Kenichi Kumasaka

{"title":"[Development of a Rapid Analytical Method for Simultaneous Determinationof Diverse Plant and Mushroom Toxins].","authors":"Toru Fukumitsu, Masahito Hagio, Kenichi Kumasaka","doi":"10.3358/shokueishi.65.172","DOIUrl":"https://doi.org/10.3358/shokueishi.65.172","url":null,"abstract":"In this study, a rapid and accurate analytical method was developed for the simultaneous determination of 26 plant toxins and 11 mushroom toxins in toxic plants, toxic mushrooms, and their cooked products using LC-MS/MS. This method enables highly selective detection of all 37 analytes, including those with high polarity and low molecular weight, within 10 min using Scherzo SS-C18 column. The analytes were extracted from the samples using methanol and trichloroacetic acid, and purified using Captiva EMR-Lipid. A 50 vol% methanol solution was used as the test solvent, to minimize matrix effects. Validation with six types of food samples demonstrated recoveries ranging from 56.0% to 180.5% (with 96% or more of analytes achieving 70-120%) and RSD of 16.0% or less (with 98% or more of analytes achieving 10% or less), at an added concentration of 1 mg/kg. These results indicate that this method is effective for health crisis management. Additionally, the method successfully detected expected toxins in food leftovers and reference products implicated in previous health hazard cases.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"172-177"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Laboratory Performance Study of the Japanese Official Method to Detect Genetically Modified Papaya Line PRSV-YK]. [检测转基因木瓜品系 PRSV-YK 的日本官方方法的实验室性能研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.61

Norihito Shibata, Toshiaki Nakasaka, Jumpei Narushima, Chie Taguchi, Miyu Sugino, Satoko Yoshiba, Keisuke Soga, Michika Kajiwara, Takaho Watanabe, Kazunari Kondo

{"title":"[Laboratory Performance Study of the Japanese Official Method to Detect Genetically Modified Papaya Line PRSV-YK].","authors":"Norihito Shibata, Toshiaki Nakasaka, Jumpei Narushima, Chie Taguchi, Miyu Sugino, Satoko Yoshiba, Keisuke Soga, Michika Kajiwara, Takaho Watanabe, Kazunari Kondo","doi":"10.3358/shokueishi.65.61","DOIUrl":"10.3358/shokueishi.65.61","url":null,"abstract":"Since the establishment of procedures for the safety assessment of food products that use recombinant DNA technology, the manufacture, import, and sale of genetically modified (GM) foods that have not undergone safety assessment are prohibited under the Food Sanitation Act. Therefore, a performance study to confirm the GM food testing operations of each laboratory is very important to ensure the reliability of the GM food monitoring system. In 2022, GM papaya line PRSV-YK-which has not yet been authorized in Japan-was selected for testing, and a papaya paste and a DNA solution were used as the test samples. With these samples, a laboratory performance study of the DNA extraction and real-time PCR operations was conducted. This confirmed that the 18 participating laboratories were generally performing the DNA extraction and real-time PCR operations correctly. However, some laboratories using certain DNA amplification reagent with some real-time PCR instruments were not able to determine the PRSV-YK detection test. This suggests that the PRSV-YK detection test may not be able to correctly detect samples containing GM papaya when performed with these combinations of instruments and reagent. In order to ensure the reliability of the PRSV-YK detection test, it is necessary to examine in detail how the combination of DNA polymerase reagents and real-time PCR instruments affects the detection limit, and to implement an appropriate solution.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 3","pages":"61-66"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Analytical Method for Melengestrol Acetate in Livestock Products Using LC-MS/MS]. [利用 LC-MS/MS 分析畜产品中醋酸美伦孕酮的方法]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.15

Takatoshi Sakai, Hiroyuki Kikuchi, Satoru Nemoto, Hiroshi Akiyama, Takaaki Taguchi, Tomoaki Tsutsumi

{"title":"[Analytical Method for Melengestrol Acetate in Livestock Products Using LC-MS/MS].","authors":"Takatoshi Sakai, Hiroyuki Kikuchi, Satoru Nemoto, Hiroshi Akiyama, Takaaki Taguchi, Tomoaki Tsutsumi","doi":"10.3358/shokueishi.65.15","DOIUrl":"10.3358/shokueishi.65.15","url":null,"abstract":"The present study verified that it is possible to analyze melengesterol acetate using the existing multi-residue method. Melengestrol acetate was extracted from livestock products using acidic acetonitrile acidified with acetic acid in the presence of n-hexane and anhydrous sodium sulfate. The crude extracts were cleaned up using an octadecylsilanized silica gel cartridge column. Separation by HPLC was performed using an octadecylsilanized silica gel column with linear gradient elution of 0.1 vol% formic acid and acetonitrile containing 0.1 vol% formic acid. For the determination of the analyte, tandem mass spectrometry with positive ion electrospray ionization was used. In recovery tests using four livestock products fortified with maximum residue limits levels of melengestrol acetate (0.001-0.02 mg/kg), the truenesses ranged from 82% to 100%, and the repeatabilities for the entire procedure ranged from 0.5 RSD% to 5.6 RSD%. In recovery tests using 11 livestock products fortified with 0.0005 mg/kg of melengestrol acetate, the truenesses ranged from 88% to 99%, and the repeatabilities ranged from 1.3 RSD% to 5.4 RSD%. The limit of quantification for melengestrol acetate in livestock products was 0.0005 mg/kg.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 1","pages":"15-19"},"PeriodicalIF":0.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Stochastic Considerations on the Upper and Lower Limits of Colony Counts on Agar Plate for Microbial Count Calculation]. 【计算微生物数量时琼脂平板菌落数量上下限的随机考虑】。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.113

Hiroshi Fujikawa

{"title":"[Stochastic Considerations on the Upper and Lower Limits of Colony Counts on Agar Plate for Microbial Count Calculation].","authors":"Hiroshi Fujikawa","doi":"10.3358/shokueishi.65.113","DOIUrl":"https://doi.org/10.3358/shokueishi.65.113","url":null,"abstract":"The upper and lower limits of microbial colony count on agar plate are essential for microbial cell calculation of food samples. In the present study, the limits presented by the three standards of Ministry of Health, Labor, and Welfare of Japan, Food and Drug Administration of US (FDA), and Japanese Industrial Standards (JIS) were studied from the stochastic point of view. Here colony counts per plate were assumed to follow the Poisson distribution from our previous studies. The probability of acceptance of a pair of colony counts, PA for cell calculation by each standard was then calculated for various values of the mean of the Poisson distribution theoretically and by simulation with random sampling numbers. The values of PA at the lower and upper limits of the standards were low. For example, PA at the mean of 25 which is the lower limit of the FDA standard was 0.278. The values of PA of colony count pairs by the three standards were demonstrated in a wide range of the mean colony count. The largest range of the mean at PA>0.5 was observed in the JIS standard among the three. A new method to determine the limits for colony counts was developed with the risk of not acceptance. These findings would be helpful to consider the limits of colony counts for cell calculation.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"113-117"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research on Safety Awareness and Current Safety Confirmation Methods for Genome-edited Foods]. [基因组编辑食品的安全意识和现行安全确认方法研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.89

Chie Taguchi, Norihito Shibata, Kazunari Kondo

引用次数: 0

[Development of a Genus Identification Method for Poisonous Plants Using Real-Time PCR]. [利用实时 PCR 开发有毒植物的属种鉴定方法]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.53

Hitoshi Miyazaki, Masaru Taniguchi

{"title":"[Development of a Genus Identification Method for Poisonous Plants Using Real-Time PCR].","authors":"Hitoshi Miyazaki, Masaru Taniguchi","doi":"10.3358/shokueishi.65.53","DOIUrl":"https://doi.org/10.3358/shokueishi.65.53","url":null,"abstract":"We have developed a rapid genus identification method for poisonous plants. The real-time PCR using the TaqMan® probe method was employed for detection, with the amplified targets being the \"trnL (UAA)-intron\" or \"trnL-trnF intergenic spacer\" regions of chloroplast DNA. The targeted plants were selected six genera (Aconitum, Colchicum, Veratrum, Brugmansia, Scopolia and Narcissus), which have been implicated in many instances of food poisoning in Japan. A tissue lysis solution was used for DNA extraction, which can be completed within approximate 30 min. A master mix corresponding to the tissue lysis solution was used for real-time PCR reagents. As a result, we were able to complete the entire process from DNA extraction to genus identification in 4 to 5 hr. The detection sensitivity was estimated at approximately 1 pg of DNA for all six plant genera. Remarkably, an amplification plot was discerned even with the crude cell lysates of all samples. It was also possible to obtain amplification curves for three plant samples that had been subjected to simulated cooking (boiling). This study suggests that the developed method can rapidly identify six genera of poisonous plants.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 3","pages":"53-60"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Determination and Residue Survey of Novel Nicotinic AcetylcholineReceptor Modulator Pesticides in Brown Rice by LC-MS/MS]. [LC-MS/MS法测定糙米中新型烟碱类乙酰胆碱受体调节剂的残留研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.118

Takayuki Nakajima, Sanae Tomizawa, Kyoko Kamijo, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Yoshihiro Ohsawa, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama

{"title":"[Determination and Residue Survey of Novel Nicotinic AcetylcholineReceptor Modulator Pesticides in Brown Rice by LC-MS/MS].","authors":"Takayuki Nakajima, Sanae Tomizawa, Kyoko Kamijo, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Yoshihiro Ohsawa, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama","doi":"10.3358/shokueishi.65.118","DOIUrl":"10.3358/shokueishi.65.118","url":null,"abstract":"Nicotinic acetylcholine receptors (nAChRs) are neurotransmitter receptors found in the nervous system of many organisms, including humans. Neonicotinoid pesticides act as nAChRs modulators that affect neurotransmission. Due to toxicity effects, their use has been restricted. However, a new class of modulators (nAChRMs) have been developed, but analytical methods for the detection of residues of these new pesticides in agricultural crops have not been established. Therefore, this study aimed to develop and validate an accurate determination method for novel nAChRMs, such as sulfoxaflor, flupyradifurone, flupyrimin, and triflumezopyrim in brown rice using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The method was applied to commercially available brown rice samples from Tokyo, Japan. Target analytes were extracted with acetonitrile, cleaned with GC/PSA, and then cleaned again with MonoSpin PBA. In accordance with the method validation guidelines for residual pesticides in foods, the performance characteristics were evaluated, with trueness ranging from 86.3% to 98.2%, repeatability of less than 6.5% relative standard deviation (RSD), and within-laboratory reproducibility of less than 6.5% RSD. These results indicate that the developed method can be applied to residue surveillance of target analytes using solvent standard calibration curves. By applying the developed method to 53 brown rice samples commercially available in Tokyo, sulfoxaflor residues were found in two samples at concentrations of 3.7 and 21.9 ng/g. This is the first report of the detection of sulfoxaflor residues in domestic agricultural products.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"118-125"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Determination of Tetrodotoxin in Miso Soup byStrong Cation Exchange Solid Phase Extraction and LC-MS/MS]. 强阳离子交换固相萃取- LC-MS/MS法测定味噌汤中的河豚毒素

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.154

Yoshiaki Fujii, Takero Kaga, Yukiko Ueda, Shiho Omae, Naoki Aoyanagi, Kazuhiko Nishimura

{"title":"[Determination of Tetrodotoxin in Miso Soup byStrong Cation Exchange Solid Phase Extraction and LC-MS/MS].","authors":"Yoshiaki Fujii, Takero Kaga, Yukiko Ueda, Shiho Omae, Naoki Aoyanagi, Kazuhiko Nishimura","doi":"10.3358/shokueishi.65.154","DOIUrl":"https://doi.org/10.3358/shokueishi.65.154","url":null,"abstract":"A method for analyzing tetrodotoxin (TTX) in miso soup samples was proposed. The samples were purified using strong cation exchange solid-phase extraction and analyzed by liquid chromatography-tandem mass spectrometry. The recovery of TTX was considerably influenced by the salt concentration in the loading solution during purification. It was observed that diluting the loading solution to reduce the salt concentration helped to maintain the recovery rate during the washing step. However, during the loading step, the benefit of dilution in improving recovery was not evident, as the enhanced retention on the solid phase caused by dilution was counteracted by losing TTX with the increased volume of the loading solution, which led to enhanced elution. This suggests that the volume of extraction solution used in the loading process is crucial in determining the recovery rate. Additionally, the use of volatile ammonium acetate as the elution solvent was explored to optimize conditions for effective recovery. Testing this method on miso soup samples with varying miso types, salt concentrations, and TTX levels resulted in consistently high recovery rates of>80%.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"154-159"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0