Food Hygiene and Safety Science最新文献_第3页

[Development and Validation of an Analytical Method for 2-Thiouracil, 4-Thiouracil, and 6-Methyl-2-thiouracil in Bovine Urine: A Method for Monitoring Inspections in Beef Exports to the European Union]. [牛尿中2-硫脲嘧啶、4-硫脲嘧啶和6-甲基-2-硫脲嘧啶分析方法的开发和验证：一种出口到欧盟的牛肉监测检查方法]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.178

Hiroko Hata, Chikako Ikegawa, Seiichiro Iizuka, Youichi Kouno, Rie Ito, Tomoaki Tsutsumi, Hiroshi Akiyama, Shizuka Saito-Shida

{"title":"[Development and Validation of an Analytical Method for 2-Thiouracil, 4-Thiouracil, and 6-Methyl-2-thiouracil in Bovine Urine: A Method for Monitoring Inspections in Beef Exports to the European Union].","authors":"Hiroko Hata, Chikako Ikegawa, Seiichiro Iizuka, Youichi Kouno, Rie Ito, Tomoaki Tsutsumi, Hiroshi Akiyama, Shizuka Saito-Shida","doi":"10.3358/shokueishi.65.178","DOIUrl":"https://doi.org/10.3358/shokueishi.65.178","url":null,"abstract":"Thiouracil (2-thiouracil) is a thyrostat used to promote weight gain in cattle. However, its use is prohibited within the European Union (EU), necessitating the monitoring of its presence in bovine urine for beef exports to the EU. In this study, we present the development and validation of a quantitative method for the determination of 2-thiouracil, 4-thiouracil, and 6-methyl-2-thiouracil in bovine urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method involves stabilizing the analytes by adding hydrochloric acid and ethylenediaminetetraacetic acid to the sample, followed by derivatization with 3-iodobenzyl bromide, cleanup with a divinylbenzene-N-vinylpyrrolidone copolymer cartridge, and subsequent LC-MS/MS analysis. The developed method was validated for determination of 2-thiouracil, 4-thiouracil, and 6-methyl-2-thiouracil in bovine urine at a concentration of 10 μg/L. The trueness ranged from 94 to 97%, with intra-day precisions below 5% and inter-day precisions below 8%. No chromatographic interference was observed near the analytes' retention times. This analytical method is particularly valuable because it can determine whether 2-thiouracil was illicitly administered or ingested via feed containing plants of the Brassicaceae family, by confirming the presence of 6-methyl-2-thiouracil or 4-thiouracil alongside 2-thiouracil in bovine urine.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"178-184"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A Study on the Scientific Reliability of Notification Data in the Foods with Function Claims]. [功能声称食品中通知数据的科学可靠性研究]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.31

Ippei Suzuki, Tsuyoshi Chiba, Kayo Yoshimatsu, Jun Takebayashi

{"title":"[A Study on the Scientific Reliability of Notification Data in the Foods with Function Claims].","authors":"Ippei Suzuki, Tsuyoshi Chiba, Kayo Yoshimatsu, Jun Takebayashi","doi":"10.3358/shokueishi.65.31","DOIUrl":"https://doi.org/10.3358/shokueishi.65.31","url":null,"abstract":"We conducted a comprehensive survey of Foods with Function Claims (FFC) submitted from April to August 2022 to examine the scientific reliability of the systematic review (SR), which is the basis for functional claims. The results of the review of 611 functional claims for 398 products showed that there were 121 functionally active substances and 87 health claims (Hc) that were labeled, with some functionally active substances having multiple functions. SRs, meta-analyses, and clinical studies were submitted as the basis of functionality for 87%, 10%, and 3% of the reports, respectively. Of these SRs, 39% of the SRs included a single paper. In 67% of the SRs with a single paper included, some of the authors of the included paper and the person who conducted the SR had the same affiliation, which raises concerns about conflicts of interest. The median of clinical trial participants in papers included for SR was relatively small, 38, and the smallest total number of SRs was 6. Thus, it was shown that there are many SRs for FFC that are based on only a single paper or a small-scale clinical trial and that lack reliability as scientific evidence.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 2","pages":"31-39"},"PeriodicalIF":0.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Comparison of DNA Extraction Methods for Processed Foods Containing Soybean or Maize]. [含有大豆或玉米的加工食品的 DNA 提取方法比较]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.25

Tomohiro Egi, Reona Takabatake, Masahiro Kishine, Keisuke Soga, Satoko Yoshiba, Norihito Shibata, Kazunari Kondo, Yasuharu Takashima

引用次数: 0

[Validation of an Analytical Method for Organochlorine Pesticide Residues in Beef Products by GC-MS Using Sampling with Increment Reduction]. [采用增量还原法取样的气相色谱-质谱牛肉产品中有机氯农药残留分析方法的验证]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.95

Tomoyuki Okada, Yuriko Koike, Tadashi Shoji, Kazuhisa Sutou

引用次数: 0

[Development of Binding Ability Assay of Lactococcus lactis Isolated from Cucumber to Mycotoxins by Surface Plasmon Resonance Imaging Assay]. [用表面等离子体共振成像法测定黄瓜乳酸乳球菌对真菌毒素结合能力的研究进展]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.160

Kenji Oonaka, Kikumi Ogihara, Shiro Miyake, Kazuo Kobayashi-Hattori, Yoshiko Sugita-Konishi

{"title":"[Development of Binding Ability Assay of Lactococcus lactis Isolated from Cucumber to Mycotoxins by Surface Plasmon Resonance Imaging Assay].","authors":"Kenji Oonaka, Kikumi Ogihara, Shiro Miyake, Kazuo Kobayashi-Hattori, Yoshiko Sugita-Konishi","doi":"10.3358/shokueishi.65.160","DOIUrl":"10.3358/shokueishi.65.160","url":null,"abstract":"Some microorganisms, including lactic acid bacteria (LAB), can bind to mycotoxins. Its binding ability is useful for mycotoxin mitigation. Conventionally, the binding assay for this ability of microorganisms to mycotoxins has been performed by the so-called in vitro assay. We previously reported that Lactococcus lactis isolated from cucumber had the ability to bind to aflatoxins using the in vitro assay., However, this is an indirect method by which binding ability is estimated from the mycotoxin residue in supernatant after some processes such as mix, incubation, and centrifugation and it takes time. In the present study, we developed a direct and rapid assay to assess their binding ability using a surface plasmon resonance imaging (SPRi) instrument. It could observe the binding ability as a visual image in real time. The efficacy of this SPRi assay was compared with the in vitro assay. Aflatoxin M1-bovine serum albumin conjugate (AFM1-BSA) and deoxynivalenol-BSA conjugate (DON-BSA) were immobilized on the surface of the sensor prism in SPRi assay. The above L. lactis was used to prove the binding ability to the aflatoxin. In vitro assay showed that the binding ratio to AFM1 was higher than that to DON in both live bacterial cells and heated cells. In the SPRi assay, the binding of live cells to AFM1 was confirmed by % of the refractivity change (%ΔR) and visual imaging in real-time. The %ΔR of DON was poor, and no visual image was recognized. On the other hand, the heated cells did not bind to any mycotoxins. The results indicate that the SPRi assay can monitor the binding ability of live cells more rapidly and simpler than in vitro assay. It would be a useful tool for the selection of beneficial mycotoxin-detoxifying LAB.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"160-166"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development of a PCR Method to Detect a Mysid Species Causing False Positives in Tests for Crustacean Allergens]. [开发一种 PCR 方法，用于检测在甲壳类过敏原检测中造成假阳性的贻贝物种]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.48

Yohei Sugano, Keiji Aotuka, Aoi Hosokawa, Tomohiro Suzuki

{"title":"[Development of a PCR Method to Detect a Mysid Species Causing False Positives in Tests for Crustacean Allergens].","authors":"Yohei Sugano, Keiji Aotuka, Aoi Hosokawa, Tomohiro Suzuki","doi":"10.3358/shokueishi.65.48","DOIUrl":"https://doi.org/10.3358/shokueishi.65.48","url":null,"abstract":"Mysids are small crustaceans that are closely related to shrimp/prawns and crabs but not subject to food allergen labeling requirements for raw materials. In the past, a processed food that contained Japanese smelt (wakasagi) was suspected of producing a false-positive result in shrimp/prawn and crab allergen test because of the presence of consumed mysids. However, there was no reported methods to confirm mysid presence. Therefore, we developed a PCR method to detect mysids. The developed PCR method had high specificity for a mysid species, with no amplification observed from samples of shrimp, crab, krill, mantis shrimp, or the meat of Japanese smelt. In addition, DNA extracted from the internal organs of Japanese smelt was amplified by this PCR method, and sequencing revealed mysid DNA. This confirmed that mysids remained in the internal organs of Japanese smelt following consumption. This PCR method for mysid detection even amplified Japanese smelt-containing processed food samples that were suspected to have produced a false-positive result in shrimp/prawn and crab ELISA. Thus, this PCR method would enable to detect such false positives are caused by mysid contamination.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 3","pages":"48-52"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Elucidation of Coloring Molecule Using a Simple Identification Method with a Color Reaction for Omphalotus japonicus]. 用一种简单的显色反应方法鉴定日本红花的显色分子。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.137

Hideyuki Shinohara, Ryuma Okawara, Ryota Akatsuka, Masahiro Sato, Yuka Nagaoka

引用次数: 0

[Improved Indophenol Titration Method for Ascorbic Acid Using a Dropper and Electronic Balance: Enhanced Convenience and Efficiency]. [使用滴管和电子天平的改进型抗坏血酸靛酚滴定法：提高便利性和效率]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.1

Keizo Umegaki, Mika Ito, Kaori Yokotani, Shizuo Yamada

{"title":"[Improved Indophenol Titration Method for Ascorbic Acid Using a Dropper and Electronic Balance: Enhanced Convenience and Efficiency].","authors":"Keizo Umegaki, Mika Ito, Kaori Yokotani, Shizuo Yamada","doi":"10.3358/shokueishi.65.1","DOIUrl":"10.3358/shokueishi.65.1","url":null,"abstract":"The analysis of ascorbic acid using the 2,6-dichloroindophenol (DCIP) titration method is a well-established technique, but requires the skilled handling of a burette for accurate measurements. In the present study, we propose a modified DCIP titrimetric method that replaces the burette with a dropper and employs an electronic balance to measure the titrated amount by weight. The dropper used can be flexibly selected, allowing for a wide range of drop sizes, from large to very small. This modification offers several advantages, including lower skill requirements, a 43% reduction in the analysis time, a 50% decrease in sample/reagent consumption, and the ability to prepare DCIP standard solutions tailored to the concentration of ascorbic acid in the sample being analyzed. Our analysis of several food samples using this improved method showed that inherent issues of the DCIP method, such as determining the titration end point, could not be resolved. Nevertheless, the improved titration method remains more convenient and adaptable than the original approach using a burette, enabling quick and accurate analysis, especially for unskilled analysts.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 1","pages":"1-6"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Quantification of Clitidine in Paralepistopsis acromelalga Using Quantitative NMR Method]. [利用定量核磁共振法对 Paralepistopsis acromelalga 中的氯替丁进行定量]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.84

Yoshitaka Ishida, Yuka Nagaoka

{"title":"[Quantification of Clitidine in Paralepistopsis acromelalga Using Quantitative NMR Method].","authors":"Yoshitaka Ishida, Yuka Nagaoka","doi":"10.3358/shokueishi.65.84","DOIUrl":"https://doi.org/10.3358/shokueishi.65.84","url":null,"abstract":"Because one toxic component of Paralepistopsis acromelalga, clitidine, is not commercially available as a reagent and because standards are difficult to obtain, a quantitative NMR method that requires no standard was investigated for this study. To compare the quantitative values obtained using the two methods, the absolute purity of the standard used for the LC-MS/MS method was calculated using quantitative NMR. The result was calculated as 89.8±1.5%: more than 10% lower than the result obtained using conventional HPLC purity. This finding is presumably attributable to the presence of water in the crystals. Calculating the absolute purity of the product before use is crucially important. The values of the Paralepistopsis acromelalga fruit extract were quantified and compared using conventional LC-MS/MS and quantitative NMR. The quantitative values did not differ significantly, suggesting that, in most cases, they were within a 5% margin of error. Furthermore, quantitative NMR provides several benefits not obtained using conventional methods, including its rapid measurement capability and its obviation of the need for a reference material for the measured substance. By virtue of these benefits, quantitative NMR is an extremely useful tool for natural toxin analysis where sudden outbreaks occur and for which rapid calculation of results is necessary.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 4","pages":"84-88"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Simultaneous Analysis of Paralepistopsis acromelalga Applied to Cooked and Processed Foods]. [熟食品和加工食品中肢端藻的同时分析]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2023-01-01 DOI: 10.3358/shokueishi.64.89

Yoshitaka Ishida, Hitoshi Ouchi, Toshiyuki Kan, Yuka Nagaoka

引用次数: 0