Food Hygiene and Safety Science最新文献_第3页

[Development of a Genus Identification Method for Poisonous Plants Using Real-Time PCR]. [利用实时 PCR 开发有毒植物的属种鉴定方法]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.53

Hitoshi Miyazaki, Masaru Taniguchi

{"title":"[Development of a Genus Identification Method for Poisonous Plants Using Real-Time PCR].","authors":"Hitoshi Miyazaki, Masaru Taniguchi","doi":"10.3358/shokueishi.65.53","DOIUrl":"https://doi.org/10.3358/shokueishi.65.53","url":null,"abstract":"We have developed a rapid genus identification method for poisonous plants. The real-time PCR using the TaqMan® probe method was employed for detection, with the amplified targets being the \"trnL (UAA)-intron\" or \"trnL-trnF intergenic spacer\" regions of chloroplast DNA. The targeted plants were selected six genera (Aconitum, Colchicum, Veratrum, Brugmansia, Scopolia and Narcissus), which have been implicated in many instances of food poisoning in Japan. A tissue lysis solution was used for DNA extraction, which can be completed within approximate 30 min. A master mix corresponding to the tissue lysis solution was used for real-time PCR reagents. As a result, we were able to complete the entire process from DNA extraction to genus identification in 4 to 5 hr. The detection sensitivity was estimated at approximately 1 pg of DNA for all six plant genera. Remarkably, an amplification plot was discerned even with the crude cell lysates of all samples. It was also possible to obtain amplification curves for three plant samples that had been subjected to simulated cooking (boiling). This study suggests that the developed method can rapidly identify six genera of poisonous plants.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 3","pages":"53-60"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Analytical Method for Melengestrol Acetate in Livestock Products Using LC-MS/MS]. [利用 LC-MS/MS 分析畜产品中醋酸美伦孕酮的方法]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.15

Takatoshi Sakai, Hiroyuki Kikuchi, Satoru Nemoto, Hiroshi Akiyama, Takaaki Taguchi, Tomoaki Tsutsumi

{"title":"[Analytical Method for Melengestrol Acetate in Livestock Products Using LC-MS/MS].","authors":"Takatoshi Sakai, Hiroyuki Kikuchi, Satoru Nemoto, Hiroshi Akiyama, Takaaki Taguchi, Tomoaki Tsutsumi","doi":"10.3358/shokueishi.65.15","DOIUrl":"10.3358/shokueishi.65.15","url":null,"abstract":"The present study verified that it is possible to analyze melengesterol acetate using the existing multi-residue method. Melengestrol acetate was extracted from livestock products using acidic acetonitrile acidified with acetic acid in the presence of n-hexane and anhydrous sodium sulfate. The crude extracts were cleaned up using an octadecylsilanized silica gel cartridge column. Separation by HPLC was performed using an octadecylsilanized silica gel column with linear gradient elution of 0.1 vol% formic acid and acetonitrile containing 0.1 vol% formic acid. For the determination of the analyte, tandem mass spectrometry with positive ion electrospray ionization was used. In recovery tests using four livestock products fortified with maximum residue limits levels of melengestrol acetate (0.001-0.02 mg/kg), the truenesses ranged from 82% to 100%, and the repeatabilities for the entire procedure ranged from 0.5 RSD% to 5.6 RSD%. In recovery tests using 11 livestock products fortified with 0.0005 mg/kg of melengestrol acetate, the truenesses ranged from 88% to 99%, and the repeatabilities ranged from 1.3 RSD% to 5.4 RSD%. The limit of quantification for melengestrol acetate in livestock products was 0.0005 mg/kg.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 1","pages":"15-19"},"PeriodicalIF":0.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research on Safety Awareness and Current Safety Confirmation Methods for Genome-edited Foods]. [基因组编辑食品的安全意识和现行安全确认方法研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.89

Chie Taguchi, Norihito Shibata, Kazunari Kondo

引用次数: 0

[Determination and Residue Survey of Novel Nicotinic AcetylcholineReceptor Modulator Pesticides in Brown Rice by LC-MS/MS]. [LC-MS/MS法测定糙米中新型烟碱类乙酰胆碱受体调节剂的残留研究]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.118

Takayuki Nakajima, Sanae Tomizawa, Kyoko Kamijo, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Yoshihiro Ohsawa, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama

{"title":"[Determination and Residue Survey of Novel Nicotinic AcetylcholineReceptor Modulator Pesticides in Brown Rice by LC-MS/MS].","authors":"Takayuki Nakajima, Sanae Tomizawa, Kyoko Kamijo, Kazuoki Yamamoto, Tomomi Takada, Yoshie Kokaji, Hiroko Shiradoh, Yoshihiro Ohsawa, Ayane Oyama, Maiko Noguchi, Tomoko Yokoyama","doi":"10.3358/shokueishi.65.118","DOIUrl":"10.3358/shokueishi.65.118","url":null,"abstract":"Nicotinic acetylcholine receptors (nAChRs) are neurotransmitter receptors found in the nervous system of many organisms, including humans. Neonicotinoid pesticides act as nAChRs modulators that affect neurotransmission. Due to toxicity effects, their use has been restricted. However, a new class of modulators (nAChRMs) have been developed, but analytical methods for the detection of residues of these new pesticides in agricultural crops have not been established. Therefore, this study aimed to develop and validate an accurate determination method for novel nAChRMs, such as sulfoxaflor, flupyradifurone, flupyrimin, and triflumezopyrim in brown rice using liquid chromatography with tandem mass spectrometry (LC-MS/MS). The method was applied to commercially available brown rice samples from Tokyo, Japan. Target analytes were extracted with acetonitrile, cleaned with GC/PSA, and then cleaned again with MonoSpin PBA. In accordance with the method validation guidelines for residual pesticides in foods, the performance characteristics were evaluated, with trueness ranging from 86.3% to 98.2%, repeatability of less than 6.5% relative standard deviation (RSD), and within-laboratory reproducibility of less than 6.5% RSD. These results indicate that the developed method can be applied to residue surveillance of target analytes using solvent standard calibration curves. By applying the developed method to 53 brown rice samples commercially available in Tokyo, sulfoxaflor residues were found in two samples at concentrations of 3.7 and 21.9 ng/g. This is the first report of the detection of sulfoxaflor residues in domestic agricultural products.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 5","pages":"118-125"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Development and Validation of an Analytical Method for 2-Thiouracil, 4-Thiouracil, and 6-Methyl-2-thiouracil in Bovine Urine: A Method for Monitoring Inspections in Beef Exports to the European Union]. [牛尿中2-硫脲嘧啶、4-硫脲嘧啶和6-甲基-2-硫脲嘧啶分析方法的开发和验证：一种出口到欧盟的牛肉监测检查方法]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.178

Hiroko Hata, Chikako Ikegawa, Seiichiro Iizuka, Youichi Kouno, Rie Ito, Tomoaki Tsutsumi, Hiroshi Akiyama, Shizuka Saito-Shida

{"title":"[Development and Validation of an Analytical Method for 2-Thiouracil, 4-Thiouracil, and 6-Methyl-2-thiouracil in Bovine Urine: A Method for Monitoring Inspections in Beef Exports to the European Union].","authors":"Hiroko Hata, Chikako Ikegawa, Seiichiro Iizuka, Youichi Kouno, Rie Ito, Tomoaki Tsutsumi, Hiroshi Akiyama, Shizuka Saito-Shida","doi":"10.3358/shokueishi.65.178","DOIUrl":"10.3358/shokueishi.65.178","url":null,"abstract":"Thiouracil (2-thiouracil) is a thyrostat used to promote weight gain in cattle. However, its use is prohibited within the European Union (EU), necessitating the monitoring of its presence in bovine urine for beef exports to the EU. In this study, we present the development and validation of a quantitative method for the determination of 2-thiouracil, 4-thiouracil, and 6-methyl-2-thiouracil in bovine urine using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method involves stabilizing the analytes by adding hydrochloric acid and ethylenediaminetetraacetic acid to the sample, followed by derivatization with 3-iodobenzyl bromide, cleanup with a divinylbenzene-N-vinylpyrrolidone copolymer cartridge, and subsequent LC-MS/MS analysis. The developed method was validated for determination of 2-thiouracil, 4-thiouracil, and 6-methyl-2-thiouracil in bovine urine at a concentration of 10 μg/L. The trueness ranged from 94 to 97%, with intra-day precisions below 5% and inter-day precisions below 8%. No chromatographic interference was observed near the analytes' retention times. This analytical method is particularly valuable because it can determine whether 2-thiouracil was illicitly administered or ingested via feed containing plants of the Brassicaceae family, by confirming the presence of 6-methyl-2-thiouracil or 4-thiouracil alongside 2-thiouracil in bovine urine.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"178-184"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Determination of Tetrodotoxin in Miso Soup byStrong Cation Exchange Solid Phase Extraction and LC-MS/MS]. 强阳离子交换固相萃取- LC-MS/MS法测定味噌汤中的河豚毒素

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.154

Yoshiaki Fujii, Takero Kaga, Yukiko Ueda, Shiho Omae, Naoki Aoyanagi, Kazuhiko Nishimura

{"title":"[Determination of Tetrodotoxin in Miso Soup byStrong Cation Exchange Solid Phase Extraction and LC-MS/MS].","authors":"Yoshiaki Fujii, Takero Kaga, Yukiko Ueda, Shiho Omae, Naoki Aoyanagi, Kazuhiko Nishimura","doi":"10.3358/shokueishi.65.154","DOIUrl":"10.3358/shokueishi.65.154","url":null,"abstract":"A method for analyzing tetrodotoxin (TTX) in miso soup samples was proposed. The samples were purified using strong cation exchange solid-phase extraction and analyzed by liquid chromatography-tandem mass spectrometry. The recovery of TTX was considerably influenced by the salt concentration in the loading solution during purification. It was observed that diluting the loading solution to reduce the salt concentration helped to maintain the recovery rate during the washing step. However, during the loading step, the benefit of dilution in improving recovery was not evident, as the enhanced retention on the solid phase caused by dilution was counteracted by losing TTX with the increased volume of the loading solution, which led to enhanced elution. This suggests that the volume of extraction solution used in the loading process is crucial in determining the recovery rate. Additionally, the use of volatile ammonium acetate as the elution solvent was explored to optimize conditions for effective recovery. Testing this method on miso soup samples with varying miso types, salt concentrations, and TTX levels resulted in consistently high recovery rates of>80%.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"154-159"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[A Study on the Scientific Reliability of Notification Data in the Foods with Function Claims]. [功能声称食品中通知数据的科学可靠性研究]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.31

Ippei Suzuki, Tsuyoshi Chiba, Kayo Yoshimatsu, Jun Takebayashi

{"title":"[A Study on the Scientific Reliability of Notification Data in the Foods with Function Claims].","authors":"Ippei Suzuki, Tsuyoshi Chiba, Kayo Yoshimatsu, Jun Takebayashi","doi":"10.3358/shokueishi.65.31","DOIUrl":"https://doi.org/10.3358/shokueishi.65.31","url":null,"abstract":"We conducted a comprehensive survey of Foods with Function Claims (FFC) submitted from April to August 2022 to examine the scientific reliability of the systematic review (SR), which is the basis for functional claims. The results of the review of 611 functional claims for 398 products showed that there were 121 functionally active substances and 87 health claims (Hc) that were labeled, with some functionally active substances having multiple functions. SRs, meta-analyses, and clinical studies were submitted as the basis of functionality for 87%, 10%, and 3% of the reports, respectively. Of these SRs, 39% of the SRs included a single paper. In 67% of the SRs with a single paper included, some of the authors of the included paper and the person who conducted the SR had the same affiliation, which raises concerns about conflicts of interest. The median of clinical trial participants in papers included for SR was relatively small, 38, and the smallest total number of SRs was 6. Thus, it was shown that there are many SRs for FFC that are based on only a single paper or a small-scale clinical trial and that lack reliability as scientific evidence.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 2","pages":"31-39"},"PeriodicalIF":0.3,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140872019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Comparison of DNA Extraction Methods for Processed Foods Containing Soybean or Maize]. [含有大豆或玉米的加工食品的 DNA 提取方法比较]。

IF 0.3 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.25

Tomohiro Egi, Reona Takabatake, Masahiro Kishine, Keisuke Soga, Satoko Yoshiba, Norihito Shibata, Kazunari Kondo, Yasuharu Takashima

引用次数: 0

[Validation of an Analytical Method for Organochlorine Pesticide Residues in Beef Products by GC-MS Using Sampling with Increment Reduction]. [采用增量还原法取样的气相色谱-质谱牛肉产品中有机氯农药残留分析方法的验证]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.95

Tomoyuki Okada, Yuriko Koike, Tadashi Shoji, Kazuhisa Sutou

引用次数: 0

[Development of Binding Ability Assay of Lactococcus lactis Isolated from Cucumber to Mycotoxins by Surface Plasmon Resonance Imaging Assay]. [用表面等离子体共振成像法测定黄瓜乳酸乳球菌对真菌毒素结合能力的研究进展]。

IF 0.2 4区农林科学

Food Hygiene and Safety Science Pub Date : 2024-01-01 DOI: 10.3358/shokueishi.65.160

Kenji Oonaka, Kikumi Ogihara, Shiro Miyake, Kazuo Kobayashi-Hattori, Yoshiko Sugita-Konishi

{"title":"[Development of Binding Ability Assay of Lactococcus lactis Isolated from Cucumber to Mycotoxins by Surface Plasmon Resonance Imaging Assay].","authors":"Kenji Oonaka, Kikumi Ogihara, Shiro Miyake, Kazuo Kobayashi-Hattori, Yoshiko Sugita-Konishi","doi":"10.3358/shokueishi.65.160","DOIUrl":"10.3358/shokueishi.65.160","url":null,"abstract":"Some microorganisms, including lactic acid bacteria (LAB), can bind to mycotoxins. Its binding ability is useful for mycotoxin mitigation. Conventionally, the binding assay for this ability of microorganisms to mycotoxins has been performed by the so-called in vitro assay. We previously reported that Lactococcus lactis isolated from cucumber had the ability to bind to aflatoxins using the in vitro assay., However, this is an indirect method by which binding ability is estimated from the mycotoxin residue in supernatant after some processes such as mix, incubation, and centrifugation and it takes time. In the present study, we developed a direct and rapid assay to assess their binding ability using a surface plasmon resonance imaging (SPRi) instrument. It could observe the binding ability as a visual image in real time. The efficacy of this SPRi assay was compared with the in vitro assay. Aflatoxin M1-bovine serum albumin conjugate (AFM1-BSA) and deoxynivalenol-BSA conjugate (DON-BSA) were immobilized on the surface of the sensor prism in SPRi assay. The above L. lactis was used to prove the binding ability to the aflatoxin. In vitro assay showed that the binding ratio to AFM1 was higher than that to DON in both live bacterial cells and heated cells. In the SPRi assay, the binding of live cells to AFM1 was confirmed by % of the refractivity change (%ΔR) and visual imaging in real-time. The %ΔR of DON was poor, and no visual image was recognized. On the other hand, the heated cells did not bind to any mycotoxins. The results indicate that the SPRi assay can monitor the binding ability of live cells more rapidly and simpler than in vitro assay. It would be a useful tool for the selection of beneficial mycotoxin-detoxifying LAB.","PeriodicalId":54373,"journal":{"name":"Food Hygiene and Safety Science","volume":"65 6","pages":"160-166"},"PeriodicalIF":0.2,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143016470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0