{"title":"Genetically modified organisms and food security in Southern Africa: conundrum and discourse.","authors":"Norman Muzhinji, Victor Ntuli","doi":"10.1080/21645698.2020.1794489","DOIUrl":"https://doi.org/10.1080/21645698.2020.1794489","url":null,"abstract":"<p><p>The importance of food security and nourishment is recognized in Southern African region and in many communities, globally. However, the attainment of food security in Southern African countries is affected by many factors, including adverse environmental conditions, pests and diseases. Scientists have been insistently looking for innovative strategies to optimize crop production and combat challenges militating against attainment of food security. In agriculture, strategies of increasing crop production include but not limited to improved crop varieties, farming practices, extension services, irrigation services, mechanization, information technology, use of fertilizers and agrochemicals. Equally important is genetic modification (GM) technology, which brings new prospects in addressing food security problems. Nonetheless, since the introduction of genetically modified crops (GMOs) three decades ago, it has been a topic of public discourse across the globe, conspicuously so in Southern African region. This is regardless of the evidence that planting GMOs positively influenced farmer's incomes, economic access to food and increased tolerance of crops to various biotic and abiotic stresses. This paper looks at the issues surrounding GMOs adoption in Southern Africa and lack thereof, the discourse, and its potential in contributing to the attainment of food security for the present as well as future generations.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"25-35"},"PeriodicalIF":3.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1794489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38179091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mingyang Zhang, Yubing Fan, Chao Chen, Jingxia Cao, Hongshan Pu
{"title":"Consumer perception, mandatory labeling, and traceability of GM soybean oil: evidence from Chinese urban consumers.","authors":"Mingyang Zhang, Yubing Fan, Chao Chen, Jingxia Cao, Hongshan Pu","doi":"10.1080/21645698.2020.1807852","DOIUrl":"https://doi.org/10.1080/21645698.2020.1807852","url":null,"abstract":"ABSTRACT Consumer preference for the mandatory labeling of genetically modified (GM) foods promotes public support for the implementation of GM food policies. This study analyzes consumers’ preference for the traceability of GM soybean oil. Survey data were collected through a self-administered survey covering 804 randomly sampled urban residents in the eastern, central and western regions of China. Using a logit model, this analysis examines the impacts of influential factors on consumers’ preference for traceability. The results show that about 56.5% of the respondents have a positive preference for the traceability of GM soybean oil. Factors increasing the preference for traceability include a better perception of the attributes of nutrition benefit and potential health risk, perceived inadequacy of simple mandatory labels, more attention paid to food labels, and distrust in the agencies overseeing GM food safety. Enhancing consumers’ perceptions of GM-related attributes and awareness of food labels will help improve the mandatory labeling management of GM foods.","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"36-46"},"PeriodicalIF":3.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1807852","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38296218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The more favorable attitude of the citizens toward GMOs supports a new regulatory framework in the European Union.","authors":"Mihael Cristin Ichim","doi":"10.1080/21645698.2020.1795525","DOIUrl":"https://doi.org/10.1080/21645698.2020.1795525","url":null,"abstract":"<p><p>Since 1996 till 2018, the global area cultivated with GM crops has increased 113-fold, making biotech crops one of the fastest adopted crop technology in the past decades. In the European Union, only two countries still cultivate one available transgenic crop event on minor hectarage. Moreover, the number of notifications for confined field trials has dramatically dropped in the last decade. All these are happening while the EU legislation on GM crops has come under severe criticism. The percentage of EU citizens concerned about the presence of GMOs in the environment has decreased from 30% (in 2002) to 19% (in 2011), while the level of concern about the use of GM ingredients in food or drinks has decreased from 63% (in 2005) to 27% (in 2019). The steadily increasing acceptance of the EU citizens of GMOs in the environment and food, as it was recorded by Eurobarometers, should additionally ease the way and support a positive change of the legal framework that regulates the GM crops' testing and commercial cultivation in the EU.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"18-24"},"PeriodicalIF":3.9,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1795525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38258406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Environmental impacts of genetically modified (GM) crop use 1996-2018: impacts on pesticide use and carbon emissions.","authors":"Graham Brookes, Peter Barfoot","doi":"10.1080/21645698.2020.1773198","DOIUrl":"https://doi.org/10.1080/21645698.2020.1773198","url":null,"abstract":"<p><p>This paper updates previous assessments of the environmental impacts associated with using crop biotechnology (specifically genetically modified crops) in global agriculture. It focuses on the environmental impacts associated with changes in pesticide use and greenhouse gas emissions arising from the use of GM crops since their first widespread commercial use 22 years ago. The adoption of GM insect resistant and herbicide tolerant technology has reduced pesticide spraying by 775.4 million kg (8.3%) and, as a result, decreased the environmental impact associated with herbicide and insecticide use on these crops (as measured by the indicator, the Environmental Impact Quotient (EIQ)) by 18.5%. The technology has also facilitated important cuts in fuel use and tillage changes, resulting in a significant reduction in the release of greenhouse gas emissions from the GM cropping area. In 2018, this was equivalent to removing 15.27 million cars from the roads.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 4","pages":"215-241"},"PeriodicalIF":3.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1773198","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38190115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amir Hameed, Muhammad Aamer Mehmood, Muhammad Shahid, Shabih Fatma, Aysha Khan, Sumbal Ali
{"title":"Prospects for potato genome editing to engineer resistance against viruses and cold-induced sweetening.","authors":"Amir Hameed, Muhammad Aamer Mehmood, Muhammad Shahid, Shabih Fatma, Aysha Khan, Sumbal Ali","doi":"10.1080/21645698.2019.1631115","DOIUrl":"https://doi.org/10.1080/21645698.2019.1631115","url":null,"abstract":"<p><p>Crop improvement through transgenic technologies is commonly tagged with GMO (genetically-modified-organisms) where the presence of transgene becomes a big question for the society and the legislation authorities. However, new plant breeding techniques like CRISPR/Cas9 system [clustered regularly interspaced palindromic repeats (CRISPR)-associated 9] can overcome these limitations through transgene-free products. Potato (<i>Solanum tuberosum</i> L.) being a major food crop has the potential to feed the rising world population. Unfortunately, the cultivated potato suffers considerable production losses due to several pre- and post-harvest stresses such as plant viruses (majorly RNA viruses) and cold-induced sweetening (CIS; the conversion of sucrose to glucose and fructose inside cell vacuole). A number of strategies, ranging from crop breeding to genetic engineering, have been employed so far in potato for trait improvement. Recently, new breeding techniques have been utilized to knock-out potato genes/factors like eukaryotic translation initiation factors [<i>elF4E</i> and isoform <i>elF(iso)4E</i>)], that interact with viruses to assist viral infection, and vacuolar invertase, a core enzyme in CIS. In this context, CRISPR technology is predicted to reduce the cost of potato production and is likely to pass through the regulatory process being marker and transgene-free. The current review summarizes the potential application of the CRISPR/Cas9 system for traits improvement in potato. Moreover, the prospects for engineering resistance against potato fungal pathogens and current limitations/challenges are discussed.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 4","pages":"185-205"},"PeriodicalIF":3.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2019.1631115","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37396412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jennifer A Anderson, James Mickelson, Mary Challender, Emily Moellring, Theresa Sult, Sarah TeRonde, Carl Walker, Yiwei Wang, Carl A Maxwell
{"title":"Agronomic and compositional assessment of genetically modified DP23211 maize for corn rootworm control.","authors":"Jennifer A Anderson, James Mickelson, Mary Challender, Emily Moellring, Theresa Sult, Sarah TeRonde, Carl Walker, Yiwei Wang, Carl A Maxwell","doi":"10.1080/21645698.2020.1770556","DOIUrl":"10.1080/21645698.2020.1770556","url":null,"abstract":"<p><p>DP23211 maize was genetically modified (GM) to express DvSSJ1 double-stranded RNA and the IPD072Aa protein for control of corn rootworm (<i>Diabrotica</i> spp.). DP23211 maize also expresses the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate herbicide, and the phosphomannose isomerase (PMI) protein that was used as a selectable marker. A multi-location field trial was conducted during the 2018 growing season at 12 sites selected to be representative of the major maize-growing regions of the U.S. and Canada. Standard agronomic endpoints as well as compositional analytes from grain and forage (e.g., proximates, fibers, minerals, amino acids, fatty acids, vitamins, anti-nutrients, secondary metabolites) were evaluated and compared to non-GM near-isoline control maize (control maize) and non-GM commercial maize (reference maize). A small number of agronomic endpoints were statistically significant compared to the control maize, but were not considered to be biologically relevant when adjusted using the false discovery rate method (FDR) or when compared to the range of natural variation established from in-study reference maize. A small number of composition analytes were statistically significant compared to the control maize. These analytes were not statistically significant when adjusted using FDR, and all analyte values fell within the range of natural variation established from in-study reference range, literature range or tolerance interval, indicating that the composition of DP23211 maize grain and forage is substantially equivalent to conventional maize represented by non-GM near-isoline control maize and non-GM commercial maize.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 4","pages":"206-214"},"PeriodicalIF":4.5,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/62/8e/KGMC_11_1770556.PMC7518745.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38056192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Compositional analysis of transgenic Bt-chickpea resistant to <i>Helicoverpa armigera</i>.","authors":"Rubi Gupta, Ananta Madhab Baruah, Sumita Acharjee, Bidyut Kumar Sarmah","doi":"10.1080/21645698.2020.1782147","DOIUrl":"10.1080/21645698.2020.1782147","url":null,"abstract":"<p><p>Transgenic chickpeas expressing high levels of a truncated version of the <i>cry1Ac</i> (tr<i>cry1Ac</i>) gene conferred complete protection to <i>Helicoverpa armigera</i> in the greenhouse. Homozygous progeny of two lines, Cry1Ac.1 and Cry1Ac.2, had similar growth pattern and other morphological characteristics, including seed yield, compared to the non-transgenic counterpart; therefore, seed compositional analysis was carried out. These selected homozygous chickpea lines were selfed for ten generations along with the non-transgenic parent under contained conditions. A comparative seed composition assessment, seed storage proteins profiling, and <i>in vitro</i> protein digestibility were performed to confirm that these lines do not have significant alterations in seed composition compared to the parent. Our analyses showed no significant difference in primary nutritional composition between transgenic and non-transgenic chickpeas. In addition, the seed storage protein profile also showed no variation between the transgenic chickpea lines. Seed protein digestibility assays using simulated gastric fluid revealed a similar rate of digestion of proteins from the transgenic tr<i>cry1Ac</i> lines compared to the non-transgenic line. Thus, our data suggest no unintended changes in the seed composition of transgenic chickpea expressing a tr<i>cry1Ac</i> gene.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 4","pages":"262-274"},"PeriodicalIF":3.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7523883/pdf/KGMC_11_1782147.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38098534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"GM crop technology use 1996-2018: farm income and production impacts.","authors":"Graham Brookes, Peter Barfoot","doi":"10.1080/21645698.2020.1779574","DOIUrl":"https://doi.org/10.1080/21645698.2020.1779574","url":null,"abstract":"<p><p>This paper estimates the global value of using genetically modified (GM) crop technology in agriculture at the farm level. It follows and updates earlier studies which examined impacts on yields, key variable costs of production, direct farm (gross) income, and impacts on the production base of the four main crops of soybeans, corn, cotton, and canola. This updated analysis shows that there continues to be very significant net economic benefits at the farm level amounting to $18.9 billion in 2018 and $225.1 billion for the period 1996-2018 (in nominal terms). These gains have been divided 52% to farmers in developing countries and 48% to farmers in developed countries. Seventy-two per cent of the gains have derived from yield and production gains with the remaining 28% coming from cost savings. The technology has also made important contributions to increasing global production levels of the four main crops, having, for example, added 278 million tonnes and 498 million tonnes, respectively, to the global production of soybeans and maize since the introduction of the technology in the mid-1990 s. In terms of investment, for each extra dollar invested in GM crop seeds (relative to the cost of conventional seed), farmers gained an average US $3.75 in extra income. In developing countries, the average return was $4.41 for each extra dollar invested in GM crop seed and in developed countries the average return was $3.24.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 4","pages":"242-261"},"PeriodicalIF":3.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1779574","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38190114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SlEAD1, an EAR motif-containing ABA down-regulated novel transcription repressor regulates ABA response in tomato.","authors":"Wei Wang, Xutong Wang, Yating Wang, Ganghua Zhou, Chen Wang, Saddam Hussain, Adnan, Rao Lin, Tianya Wang, Shucai Wang","doi":"10.1080/21645698.2020.1790287","DOIUrl":"https://doi.org/10.1080/21645698.2020.1790287","url":null,"abstract":"<p><p>EAR motif-containing proteins are able to repress gene expression, therefore play important roles in regulating plants growth and development, plant response to environmental stimuli, as well as plant hormone signal transduction. ABA is a plant hormone that regulates abiotic stress tolerance in plants via signal transduction. ABA signaling via the PYR1/PYLs/RCARs receptors, the PP2Cs phosphatases, and SnRK2s protein kinases activates the ABF/AREB/ABI5-type bZIP transcription factors, resulting in the activation/repression of ABA response genes. However, functions of many ABA response genes remained largely unknown. We report here the identification of the ABA-responsive gene <i>SlEAD1</i> (<i>Solanum lycopersicum EAR motif-containing ABA down-regulated 1</i>) as a novel EAR motif-containing transcription repressor gene in tomato. We found that the expression of <i>SlEAD1</i> was down-regulated by ABA treatment, and SlEAD1 repressed reporter gene expression in transfected protoplasts. By using CRISPR gene editing, we generated transgene-free <i>slead1</i> mutants and found that the mutants produced short roots. By using seed germination and root elongation assays, we examined ABA response of the <i>slead1</i> mutants and found that ABA sensitivity in the mutants was increased. By using qRT-PCR, we further show that the expression of some of the ABA biosynthesis and signaling component genes were increased in the <i>slead1</i> mutants. Taken together, our results suggest that <i>SlEAD1</i> is an ABA response gene, that SlEAD1 is a novel EAR motif-containing transcription repressor, and that SlEAD1 negatively regulates ABA responses in tomato possibly by repressing the expression of some ABA biosynthesis and signaling genes.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 4","pages":"275-289"},"PeriodicalIF":3.9,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1790287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38187510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Detection of genetically modified maize in Jordan.","authors":"Abeer Aburumman, Hussein Migdadi, Muhanad Akash, Ayed Al-Abdallat, Yaser Hassan Dewir, Muhammad Farooq","doi":"10.1080/21645698.2020.1747353","DOIUrl":"https://doi.org/10.1080/21645698.2020.1747353","url":null,"abstract":"<p><p>This study aimed to detect genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. We used promoter <i>35 S</i> and <i>T-nos</i> terminator for general screening of transgenic materials. Conventional PCR detected the specific events for the screening of Bt 11, MON810, and Bt176 events. Seeds of eleven maize hybrids samples showed a positive response to the <i>35 S</i> promoter; nine out of eleven showed a positive response for <i>T-nos</i> terminator. Bt11 event was the most used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed the presence of the Bt176 event; however, MON810 not detected in any of the tested hybrids. We studied the Bt11 event in imported GMM seeds in Jordan for the first time, reinforcing the need for a mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 3","pages":"164-170"},"PeriodicalIF":3.9,"publicationDate":"2020-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1747353","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37812346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}