{"title":"Correction.","authors":"","doi":"10.1080/21645698.2021.1843218","DOIUrl":"https://doi.org/10.1080/21645698.2021.1843218","url":null,"abstract":"","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"145"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1843218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38685328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Alteration of resveratrol-dependent glycosyltransferase activity by elicitation in DJ-526 rice.","authors":"Vipada Kantayos, Jin-Suk Kim, So-Hyeon Baek","doi":"10.1080/21645698.2020.1859314","DOIUrl":"https://doi.org/10.1080/21645698.2020.1859314","url":null,"abstract":"<p><p>Since the successful creation of DJ-526, a resveratrol-enriched rice cultivar, research has focused on resveratrol production because of its great potential in pharmaceutical applications. However, the utilization of resveratrol in DJ-526 is limited by glycosylation, which converts resveratrol to its glucoside (piceid), in a process driven by glycosyltransferase. The verification of resveratrol-dependent glycosyltransferase activity is an essential strategy for improving resveratrol production in DJ-526 rice. In this study, 27 candidate glycosyltransferases were evaluated in germinated seeds. Among the candidates, only R12 exhibited upregulation related to increased resveratrol and piceid content during seed germination, whereas various effects on the activity of glycosyltransferase were observed by the presence of a bio-elicitor. Yeast extract tended to enhance glycosyltransferase activity by seven candidates, and a specific peak for an unknown compound production was identified. Conversely, chitosan acted as a glycosyltransferase inhibitor. Our results suggested that R12 and R19 are the most relevant candidate resveratrol-dependent glycosyltransferases in DJ-526 seeds during germination and elicitation. Future research should assess the possibility of silencing these candidate genes in an effort to improve resveratrol levels in DJ-526 rice.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"242-250"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1859314","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38778579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Correction.","authors":"","doi":"10.1080/21645698.2021.1956203","DOIUrl":"https://doi.org/10.1080/21645698.2021.1956203","url":null,"abstract":"","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"434"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1956203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39223023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gideon Sadikiel Mmbando, Mika Teranishi, Jun Hidema
{"title":"Transgenic rice <i>Oryza glaberrima</i> with higher CPD photolyase activity alleviates UVB-caused growth inhibition.","authors":"Gideon Sadikiel Mmbando, Mika Teranishi, Jun Hidema","doi":"10.1080/21645698.2021.1977068","DOIUrl":"https://doi.org/10.1080/21645698.2021.1977068","url":null,"abstract":"<p><p>The ultraviolet B (UVB) sensitivity of rice cultivated in Asia and Africa varies greatly, with African rice cultivars (<i>Oryza glaberrima</i> Steud. and <i>O. barthii</i> A. Chev.) being more sensitive to UVB because of their low cyclobutane pyrimidine dimer (CPD) photolyase activity, which is a CPD repair enzyme, relative to Asian rice cultivars (<i>O. sativa</i> L.). Hence, the production of UVB-resistant African rice with augmented CPD photolyase activity is of great importance, although difficulty in transforming the African rice cultivars to this end has been reported. Here, we successfully produced overexpressing transgenic African rice with higher CPD photolyase activity by modifying media conditions for callus induction and regeneration using the parental line (PL), UVB-sensitive African rice TOG12380 (<i>O. glaberrima</i>). The overexpressing transgenic African rice carried a single copy of the CPD photolyase enzyme, with a 4.4-fold higher level of CPD photolyase transcripts and 2.6-fold higher activity than its PL counterpart. When the plants were grown for 21 days in a growth chamber under visible radiation or with supplementary various UVB radiation, the overexpressing transgenic plants have a significantly increased UVB resistance index compared to PL plants. These results strongly suggest that CPD photolyase remains an essential factor for tolerating UVB radiation stress in African rice. As a result, African rice cultivars with overexpressed CPD photolyase may survive better in tropical areas more prone to UVB radiation stress, including Africa. Collectively, our results provide strong evidence that CPD photolyase is a useful biotechnological tool for reducing UVB-induced growth inhibition in African rice crops of <i>O. glaberrima</i>.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"435-448"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39747202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhila Osmani, Mohammad Sadegh Sabet, Kenji S Nakahara, Ali Mokhtassi-Bidgoli, Khabat Vahabi, Ahmad Moieni, Masoud Shams-Bakhsh
{"title":"Identification of a defense response gene involved in signaling pathways against PVA and PVY in potato.","authors":"Zhila Osmani, Mohammad Sadegh Sabet, Kenji S Nakahara, Ali Mokhtassi-Bidgoli, Khabat Vahabi, Ahmad Moieni, Masoud Shams-Bakhsh","doi":"10.1080/21645698.2020.1823776","DOIUrl":"https://doi.org/10.1080/21645698.2020.1823776","url":null,"abstract":"<p><p>Potato is the most important non-grain food crop in the world. Viruses, particularly potato virus Y (PVY) and potato virus A (PVA), are among the major agricultural pathogens causing severe reduction in potato yield and quality worldwide. Virus infection induces host factors to interfere with its infection cycle. Evaluation of these factors facilitates the development of intrinsic resistance to plant viruses. In this study, a small G-protein as one of the critical signaling factors was evaluated in plant response to PVY and PVA to enhance resistance. For this purpose, the gene expression dataset of G-proteins in potato plant under five biotic (viruses, bacteria, fungi, nematodes, and insects) and four abiotic (cold, heat, salinity, and drought) stress conditions were collected from gene expression databases. We reduced the number of the selected G-proteins to a single protein, <i>StSAR1A</i>, which is possibly involved in virus inhibition. <i>StSAR1A</i> overexpressed transgenic plants were created via the Agrobacterium-mediated method. Real-time PCR and Enzyme-linked immunosorbent assay tests of transgenic plants mechanically inoculated with PVY and PVA indicated that the overexpression of <i>StSAR1A</i> gene enhanced resistance to both viruses. The virus-infected transgenic plants exhibited a greater stem length, a larger leaf size, a higher fresh/dry weight, and a greater node number than those of the wild-type plants. The maximal photochemical efficiency of photosystem II, stomatal conductivity, and net photosynthetic rate in the virus-infected transgenic plants were also obviously higher than those of the control. The present study may help to understand aspects of resistance against viruses.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"86-105"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1823776","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38467334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sara Hejri, Azam Salimi, Mohammad Ali Malboobi, Foad Fatehi
{"title":"Comparative proteome analyses of rhizomania resistant transgenic sugar beets based on RNA silencing mechanism.","authors":"Sara Hejri, Azam Salimi, Mohammad Ali Malboobi, Foad Fatehi","doi":"10.1080/21645698.2021.1954467","DOIUrl":"https://doi.org/10.1080/21645698.2021.1954467","url":null,"abstract":"<p><p>Rhizomania is an economically important disease of sugar beet, which is caused by <i>Beet necrotic yellow vein virus</i> (BNYVV). As previously shown, RNA silencing mechanism effectively inhibit the viral propagation in transgenic sugar beet plants. To investigate possible proteomic changes induced by gene insertion and/or RNA silencing mechanism, the root protein profiles of wild type sugar beet genotype 9597, as a control, and transgenic events named 6018-T3:S6-44 (S6) and 219-T3:S3-13.2 (S3) were compared by two-dimensional gel electrophoresis. The accumulation levels of 25 and 24 proteins were differentially regulated in S3 and S6 plants, respectively. The accumulation of 15 spots were increased or decreased more than 2-fold. Additionally, 10 spots repressed or induced in both, while seven spots showed variable results in two events. All the differentially expressed spots were analyzed by MALDI-TOF-TOF mass spectrometry. The functional analysis of differentially accumulated proteins showed that most of them are related to the metabolism and defense/stress response. None of these recognized proteins were allergens or toxic proteins except for a spot identified as phenylcoumaran benzylic ether reductase, Pyrc5, which was decreased in the genetically modified S6 plant. These data are in favor of substantial equivalence of the transgenic plants in comparison to their related wild type cultivar since the proteomic profile of sugar beet root was not remarkably affected by gene transfer and activation RNA silencing mechanism.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"419-433"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39394299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdul Razzaq, Arfan Ali, Muhammad Mubashar Zafar, Aisha Nawaz, Deng Xiaoying, Li Pengtao, Ge Qun, Muhammad Ashraf, Maozhi Ren, Wankui Gong, Yuan Youlu
{"title":"Pyramiding of <i>cry</i> toxins and methanol producing genes to increase insect resistance in cotton.","authors":"Abdul Razzaq, Arfan Ali, Muhammad Mubashar Zafar, Aisha Nawaz, Deng Xiaoying, Li Pengtao, Ge Qun, Muhammad Ashraf, Maozhi Ren, Wankui Gong, Yuan Youlu","doi":"10.1080/21645698.2021.1944013","DOIUrl":"https://doi.org/10.1080/21645698.2021.1944013","url":null,"abstract":"<p><p>The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression of <i>cry</i> genes from <i>Bacillus thuringiensis</i> as a strategy to improve insect pest control in cotton is presented. We constructed a cassette containing two <i>cry</i> genes (<i>cry1Fa</i> and <i>Cry32Aa</i>) and two <i>pme</i> genes, one from <i>Arabidopsis thaliana</i> (<i>AtPME</i>), and other from <i>Aspergillus. niger</i> (<i>AnPME</i>) in pCAMBIA1301 plant expression vector using CAMV-35S promoter. This construction was transformed in Eagle-2 cotton variety by using shoot apex-cut <i>Agrobacterium</i>-mediated transformation. Expression of <i>cry</i> genes and <i>pme</i> genes was confirmed by qPCR. Methanol production was measured in control and in the <i>cry</i> and <i>pme</i> transformed plants showing methanol production only in transformed plants, in contrast to the non-transgenic cotton plants. Finally, insect bioassays performed with transgenic plants expressing <i>cry</i> and <i>pme</i> genes showed 100% mortality for <i>Helicoverpa armigera</i> (cotton bollworm) larvae, 70% mortality for <i>Pectinophora gossypiella</i> (pink bollworm) larvae and 95% mortality of <i>Earias fabia</i>, (spotted bollworm) larvae, that was higher than the transgenic plants expressing only <i>cry</i> genes that showed 84%, 49% and 79% mortality, respectively. These results demonstrate that Bt. <i>cry</i>-genes coupled with <i>pme</i> genes are an effective strategy to improve the control of different insect pests.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"382-395"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1944013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39125787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Swedish consumers´ attitudes and values to genetic modification and conventional plant breeding - The case of fruit and vegetables.","authors":"Sara Spendrup, Dennis Eriksson, Fredrik Fernqvist","doi":"10.1080/21645698.2021.1921544","DOIUrl":"https://doi.org/10.1080/21645698.2021.1921544","url":null,"abstract":"<p><p>This study examined public attitudes to genetic modification (GM) and conventional plant breeding and explored general differences in attitudes to these two types of breeding concepts, including the effect of individual personal characteristics such as gender and age. It also sought to identify the influence of personal values linked to attitudes to GM crops and conventional plant breeding, following Schwartz value theory. Relations between specific values and attitudes to GM organisms (GMOs) have been studied previously, but not gender- and age-specific relations between specific values and attitudes to conventional plant breeding. Data were collected in this study using a questionnaire completed on-line by 1500 Swedish consumers in 2019. The questionnaire covered three different aspects: 1) sociodemographic data, including gender and age; 2) attitudes to GMO/conventional plant breeding; and 3) values, measured using the human values scale. It was found that consumers expressed more positive attitudes to conventional plant breeding than to GMO, men expressed more positive attitudes to both conventional plant breeding and GMO than women did, and younger consumers expressed more positive attitudes to GMO than older consumers did. A negative correlation between attitudes to conventional plant breeding and the value 'tradition', but no correlation to 'universalism', 'benevolence', 'power' or 'achievement', was identified for men. For women, correlations between attitudes to conventional plant breeding and 'benevolence' (neg.) and 'achievement' (pos.) were found. For both men and women, attitudes to GMO were negatively influenced by 'universalism' and 'benevolence', and positively influenced by 'power' and 'achievement'. The implications of these results are discussed.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"342-360"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1921544","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38886778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Waseem, Mehtab Muhammad Aslam, Iffat Shaheen
{"title":"The DUF221 domain-containing (DDP) genes identification and expression analysis in tomato under abiotic and phytohormone stress.","authors":"Muhammad Waseem, Mehtab Muhammad Aslam, Iffat Shaheen","doi":"10.1080/21645698.2021.1962207","DOIUrl":"https://doi.org/10.1080/21645698.2021.1962207","url":null,"abstract":"<p><p>The domain of unknown function (DUF221 domain-containing) proteins regulates various aspects of plant growth, development, responses to abiotic stresses, and hormone transduction pathways. To understand the role of DDP proteins in tomato, a comprehensive genome-wide analysis was performed in the tomato genome. A total of 12 DDP genes were identified and distributed in 8 chromosomes in the tomato genome. Phylogenetically all SlDDPs were clustered into four clades, subsequently supported by their gene structure and conserved motifs distribution. The SlDDPs contained various cis-acting elements involved in plant responses to abiotic and various phytohormones stresses. The tissue-specific expression profile analysis revealed the constitutive expression of <i>SlDDPs</i> in roots, leaves, and developmental phases of fruit. It was found that <i>SlDDP1, SlDDP3, SlDDP4, SlDDP9, SlDDP10</i>, and <i>SlDDP12</i> exhibited high expression levels in fruits at different development stages. Of these genes, <i>SlDDP12</i> contained ethylene (ERE) responsive elements in their promoter regions, suggesting its role in ethylene-dependent fruit ripening. It was found that a single SlDDP induced by two or more abiotic and phytohormone stresses. These include, <i>SlDDP1, SlDDP2, SlDDP3, SlDDP4, SlDDP7, SlDDP8</i>, and <i>SlDDP10</i> was induced under salt, drought, ABA, and IAA stresses. Moreover, tomato SlDDPs were targeted by multiple miRNA gene families as well. In conclusion, this study predicted that the putative DDP genes might help improve abiotic and phytohormone tolerance in plants, particularly tomato, rice, and other economically important crop plant species.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"586-599"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8820248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39300311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Waseem, Feiyan Huang, Qiyu Wang, Mehtab Muhammad Aslam, Farhat Abbas, Fiaz Ahmad, Umair Ashraf, Waseem Hassan, Sajid Fiaz, Xianwen Ye, Lei Yu, Yanguo Ke
{"title":"Identification, methylation profiling, and expression analysis of stress-responsive cytochrome P450 genes in rice under abiotic and phytohormones stresses.","authors":"Muhammad Waseem, Feiyan Huang, Qiyu Wang, Mehtab Muhammad Aslam, Farhat Abbas, Fiaz Ahmad, Umair Ashraf, Waseem Hassan, Sajid Fiaz, Xianwen Ye, Lei Yu, Yanguo Ke","doi":"10.1080/21645698.2021.1908813","DOIUrl":"https://doi.org/10.1080/21645698.2021.1908813","url":null,"abstract":"<p><p>The cytochrome P450 (CYP) is a large and complex eukaryotic gene superfamily with enzymatic activities involved in several physiological and regulatory processes. As an objective, an <i>in-silico</i> genome-wide DNA methylation (5mC) analysis was performed in rice (<i>Oryza sativa</i> cv. Zhonghua11), and the epigenetic role of CYPs in two abiotic stresses was observed. Being a stable representative mark, DNA-methylation alters the gene expression under stressful environmental conditions. Rice plants under salinity and drought stresses were analyzed through MeDIP-chip hybridization, and 14 unique genes of the CYP family were identified in the rice genome with varying degrees of methylation. The gene structure, promoter sequences, and phylogenetic analysis were performed. Furthermore, the responses of CYPs to various abiotic stresses, including salinity, drought, and cold were revealed. Similarly, the expression profile of potential CYPs was also investigated under various phytohormone stresses, which revealed the potential involvement of CYPs to hormone regulations. Overall, the current study provides evidence for CYP's stress regulation and fundamental for further characterization and understanding their epigenetic roles in gene expression regulation and environmental stress regulation in higher plants.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"12 1","pages":"551-563"},"PeriodicalIF":3.9,"publicationDate":"2021-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2021.1908813","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38891001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}