Transgenic rice Oryza glaberrima with higher CPD photolyase activity alleviates UVB-caused growth inhibition.

IF 4.5 2区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Gideon Sadikiel Mmbando, Mika Teranishi, Jun Hidema
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引用次数: 8

Abstract

The ultraviolet B (UVB) sensitivity of rice cultivated in Asia and Africa varies greatly, with African rice cultivars (Oryza glaberrima Steud. and O. barthii A. Chev.) being more sensitive to UVB because of their low cyclobutane pyrimidine dimer (CPD) photolyase activity, which is a CPD repair enzyme, relative to Asian rice cultivars (O. sativa L.). Hence, the production of UVB-resistant African rice with augmented CPD photolyase activity is of great importance, although difficulty in transforming the African rice cultivars to this end has been reported. Here, we successfully produced overexpressing transgenic African rice with higher CPD photolyase activity by modifying media conditions for callus induction and regeneration using the parental line (PL), UVB-sensitive African rice TOG12380 (O. glaberrima). The overexpressing transgenic African rice carried a single copy of the CPD photolyase enzyme, with a 4.4-fold higher level of CPD photolyase transcripts and 2.6-fold higher activity than its PL counterpart. When the plants were grown for 21 days in a growth chamber under visible radiation or with supplementary various UVB radiation, the overexpressing transgenic plants have a significantly increased UVB resistance index compared to PL plants. These results strongly suggest that CPD photolyase remains an essential factor for tolerating UVB radiation stress in African rice. As a result, African rice cultivars with overexpressed CPD photolyase may survive better in tropical areas more prone to UVB radiation stress, including Africa. Collectively, our results provide strong evidence that CPD photolyase is a useful biotechnological tool for reducing UVB-induced growth inhibition in African rice crops of O. glaberrima.

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具有较高CPD光解酶活性的转基因水稻glaberrima减轻了uvb引起的生长抑制。
亚洲和非洲栽培的水稻对紫外线B (UVB)的敏感性差异很大,非洲栽培的水稻品种(Oryza glaberrima Steud;与亚洲水稻品种(O. sativa L.)相比,水稻品种(O. sativa L.)的CPD修复酶环丁烷嘧啶二聚体(cyclobuty烷嘧啶二聚体,CPD)光解酶活性较低,对UVB更加敏感。因此,生产具有增强CPD光解酶活性的抗uvb的非洲水稻是非常重要的,尽管有报道称在这方面改造非洲水稻品种很困难。本研究以uvb敏感非洲稻TOG12380为材料,通过改变愈伤组织诱导和再生的培养基条件,成功培育出CPD光解酶活性较高的过表达转基因非洲稻。过表达的转基因非洲稻携带CPD光解酶单拷贝,其CPD光解酶转录物水平比PL高4.4倍,活性比PL高2.6倍。当植株在可见辐射或补充各种UVB辐射的生长室中生长21 d时,过表达转基因植株的抗UVB指数明显高于PL植株。这些结果强烈表明,CPD光解酶仍然是非洲水稻耐受UVB辐射胁迫的重要因素。因此,CPD光解酶过表达的非洲水稻品种可能在包括非洲在内的热带地区更容易受到UVB辐射胁迫,从而更好地生存。总之,我们的研究结果提供了强有力的证据,证明CPD光解酶是一种有用的生物技术工具,可以减少uvb诱导的非洲水稻O. glaberrima的生长抑制。
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来源期刊
Gm Crops & Food-Biotechnology in Agriculture and the Food Chain
Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
8.10
自引率
10.30%
发文量
22
期刊介绍: GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments
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