The DUF221 domain-containing (DDP) genes identification and expression analysis in tomato under abiotic and phytohormone stress.

IF 4.5 2区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2021-01-02 Epub Date: 2021-08-11 DOI:10.1080/21645698.2021.1962207

Muhammad Waseem, Mehtab Muhammad Aslam, Iffat Shaheen

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引用次数: 5

Abstract

The domain of unknown function (DUF221 domain-containing) proteins regulates various aspects of plant growth, development, responses to abiotic stresses, and hormone transduction pathways. To understand the role of DDP proteins in tomato, a comprehensive genome-wide analysis was performed in the tomato genome. A total of 12 DDP genes were identified and distributed in 8 chromosomes in the tomato genome. Phylogenetically all SlDDPs were clustered into four clades, subsequently supported by their gene structure and conserved motifs distribution. The SlDDPs contained various cis-acting elements involved in plant responses to abiotic and various phytohormones stresses. The tissue-specific expression profile analysis revealed the constitutive expression of SlDDPs in roots, leaves, and developmental phases of fruit. It was found that SlDDP1, SlDDP3, SlDDP4, SlDDP9, SlDDP10, and SlDDP12 exhibited high expression levels in fruits at different development stages. Of these genes, SlDDP12 contained ethylene (ERE) responsive elements in their promoter regions, suggesting its role in ethylene-dependent fruit ripening. It was found that a single SlDDP induced by two or more abiotic and phytohormone stresses. These include, SlDDP1, SlDDP2, SlDDP3, SlDDP4, SlDDP7, SlDDP8, and SlDDP10 was induced under salt, drought, ABA, and IAA stresses. Moreover, tomato SlDDPs were targeted by multiple miRNA gene families as well. In conclusion, this study predicted that the putative DDP genes might help improve abiotic and phytohormone tolerance in plants, particularly tomato, rice, and other economically important crop plant species.

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非生物和植物激素胁迫下番茄DUF221结构域(DDP)基因的鉴定与表达分析

未知功能域(含DUF221结构域)蛋白调节植物生长发育、非生物胁迫反应和激素转导途径的各个方面。为了了解DDP蛋白在番茄中的作用，对番茄基因组进行了全面的全基因组分析。共鉴定出12个DDP基因，分布在番茄基因组的8条染色体上。在系统发育上，所有sldps均聚为4个支系，随后被其基因结构和保守基序分布所支持。sldps含有多种顺式作用元件，参与植物对非生物和各种植物激素胁迫的反应。组织特异性表达谱分析揭示了sldps在根、叶和果实发育阶段的组成性表达。结果表明，SlDDP1、SlDDP3、SlDDP4、SlDDP9、SlDDP10和SlDDP12在不同发育阶段的果实中表达量较高。在这些基因中，SlDDP12在其启动子区域含有乙烯(ERE)响应元件，表明其在乙烯依赖的果实成熟中起作用。发现单株SlDDP可由两种或多种非生物和植物激素胁迫诱导。其中SlDDP1、SlDDP2、SlDDP3、SlDDP4、SlDDP7、SlDDP8和SlDDP10在盐、干旱、ABA和IAA胁迫下诱导。此外，番茄sldps也被多个miRNA基因家族靶向。总之，本研究预测，推测的DDP基因可能有助于提高植物的非生物和植物激素耐受性，特别是番茄、水稻和其他经济上重要的作物植物物种。

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来源期刊

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

8.10

自引率

10.30%

发文量

期刊介绍： GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments