Pyramiding of cry toxins and methanol producing genes to increase insect resistance in cotton.

IF 4.5 2区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Pub Date : 2021-01-02 DOI:10.1080/21645698.2021.1944013

Abdul Razzaq, Arfan Ali, Muhammad Mubashar Zafar, Aisha Nawaz, Deng Xiaoying, Li Pengtao, Ge Qun, Muhammad Ashraf, Maozhi Ren, Wankui Gong, Yuan Youlu

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引用次数: 8

Abstract

The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression of cry genes from Bacillus thuringiensis as a strategy to improve insect pest control in cotton is presented. We constructed a cassette containing two cry genes (cry1Fa and Cry32Aa) and two pme genes, one from Arabidopsis thaliana (AtPME), and other from Aspergillus. niger (AnPME) in pCAMBIA1301 plant expression vector using CAMV-35S promoter. This construction was transformed in Eagle-2 cotton variety by using shoot apex-cut Agrobacterium-mediated transformation. Expression of cry genes and pme genes was confirmed by qPCR. Methanol production was measured in control and in the cry and pme transformed plants showing methanol production only in transformed plants, in contrast to the non-transgenic cotton plants. Finally, insect bioassays performed with transgenic plants expressing cry and pme genes showed 100% mortality for Helicoverpa armigera (cotton bollworm) larvae, 70% mortality for Pectinophora gossypiella (pink bollworm) larvae and 95% mortality of Earias fabia, (spotted bollworm) larvae, that was higher than the transgenic plants expressing only cry genes that showed 84%, 49% and 79% mortality, respectively. These results demonstrate that Bt. cry-genes coupled with pme genes are an effective strategy to improve the control of different insect pests.

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棉花抗虫毒素和甲醇基因的聚合。

提出了利用果胶甲基酯酶(PME)结合苏云金芽孢杆菌(Bacillus thuringiensis) cry基因的表达提高细胞壁甲醇产量的策略，以提高棉花害虫的防治效果。我们构建了一个包含两个哭泣基因(cry1Fa和Cry32Aa)和两个pme基因的磁带，一个来自拟南芥(AtPME)，另一个来自曲霉。利用CAMV-35S启动子在pCAMBIA1301植物表达载体上表达AnPME。利用农杆菌介导的茎尖切型转化，将该结构转化为Eagle-2棉花品种。通过qPCR证实了cry基因和pme基因的表达。在对照、cry和pme转化植株中测量了甲醇产量，结果显示，与非转基因棉花植株相比，只有转化植株产生甲醇。最后，用表达cry和pme基因的转基因植物进行昆虫生物测定，结果显示，棉铃虫(Helicoverpa armigera)幼虫的死亡率为100%，粉红棉铃虫(Pectinophora gossypiella)幼虫的死亡率为70%，斑点棉铃虫(Earias fabia)幼虫的死亡率为95%，均高于仅表达cry基因的转基因植物(84%，49%和79%)。这些结果表明，Bt. cry基因与pme基因联用是一种有效的害虫防治策略。

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来源期刊

Gm Crops & Food-Biotechnology in Agriculture and the Food Chain Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

8.10

自引率

10.30%

发文量

期刊介绍： GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers. GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer. Topics covered include, but are not limited to: • Production and analysis of transgenic crops • Gene insertion studies • Gene silencing • Factors affecting gene expression • Post-translational analysis • Molecular farming • Field trial analysis • Commercialization of modified crops • Safety and regulatory affairs BIOLOGICAL SCIENCE AND TECHNOLOGY • Biofuels • Data from field trials • Development of transformation technology • Elimination of pollutants (Bioremediation) • Gene silencing mechanisms • Genome Editing • Herbicide resistance • Molecular farming • Pest resistance • Plant reproduction (e.g., male sterility, hybrid breeding, apomixis) • Plants with altered composition • Tolerance to abiotic stress • Transgenesis in agriculture • Biofortification and nutrients improvement • Genomic, proteomic and bioinformatics methods used for developing GM cops ECONOMIC, POLITICAL AND SOCIAL ISSUES • Commercialization • Consumer attitudes • International bodies • National and local government policies • Public perception, intellectual property, education, (bio)ethical issues • Regulation, environmental impact and containment • Socio-economic impact • Food safety and security • Risk assessments