Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Genomic characterisation of Actinospongicola halichondriae gen. nov., sp. nov., the first sponge-derived cultivated representative of a new genus within the class Acidimicrobiia","authors":"Jing Huang,&nbsp;Jutta Wiese,&nbsp;Leon X. Steiner,&nbsp;Tanja Rahn,&nbsp;Erik Borchert,&nbsp;Ute Hentschel","doi":"10.1007/s10482-025-02126-4","DOIUrl":"10.1007/s10482-025-02126-4","url":null,"abstract":"<div><p>A new strain (named Hal317^T) of class <i>Acidimicrobiia</i> was isolated from the marine breadcrumb sponge <i>Halichondria panicea</i> sampled in the Kiel Fjord (Baltic Sea, Germany). Phylogenetic analysis of the 16S rRNA gene sequence revealed similarities from 84.86 to 91.54% to all known type strains of the class <i>Acidimicrobiia</i>. Genome comparisons of strain Hal317^T with type strains of the family <i>Iamiaceae</i> exhibited average nucleotide identities (ANI) &lt; 71.49% and percentage of conserved proteins (POCP) values &lt; 50.30%. Strain Hal317^T was determined to be microaerophilic. Optimal growth occurred at 30–35 °C, 1–2% sea salt, and pH 6–7. Major fatty acids identified were C_18:1 <i>ω</i>7<i>c</i>, C_16:1 <i>ω</i>7<i>c</i>, C_17:1 <i>ω</i>8<i>c</i>, and C_16:0. The major respiratory menaquinone was MK-9(H₈). The G + C content of strain Hal317^T was 68.70%. Based on the polyphasic approach of bacterial classification, strain Hal317^T could be distinguished from known genera of the family <i>Iamiaceae</i>, and we propose the name <i>Actinospongicola halichondriae</i> gen. nov., sp. nov. (= DSM 114536^T = LMG 32795^T). Genomic features indicated that strain Hal317^T was characteristic of a sponge-associated lifestyle, such as the potential to metabolise diverse sponge-derived carbohydrates (e.g. chitin, <span>l</span>-fucose) and sulfur compounds (e.g. taurine). Moreover, the genome of strain Hal317^T contained stress response-related genes, including those involved in ectoine synthesis and toxin-antitoxin systems. Within the family <i>Iamiaceae</i> strain Hal317^T revealed unique genomic features, including a non-phosphorylated <span>l</span>-fucose pathway. Our study sheds light on the <i>Acidimicrobiia</i>, a class typified by few cultured representatives. Additionally, the new sponge-derived isolate offers valuable insights into the host-bacteria association.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12263773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haematococcus pluvialis culture contaminated with chytrids: growth management and astaxanthin production","authors":"Zineb Mansouri,&nbsp;Ibtissam Lijassi,&nbsp;Ana Amorim,&nbsp;Aïcha El Aissami,&nbsp;Laila Rhazi,&nbsp;Imane Wahby","doi":"10.1007/s10482-025-02129-1","DOIUrl":"10.1007/s10482-025-02129-1","url":null,"abstract":"<div><p><i>Haematococcus pluvialis</i> Flotow has gained growing interest last years due to its high content of astaxanthin. The quick expansion of its culture worldwide has been accompanied with report of several cases of fungal contamination, mainly with chytrids, causing serious losses in astaxanthin production. Although chemical fungicides efficiently eliminate chytrids contamination, they also affect algal growth. The aim of this study was to test the efficiency of chytrids’ contamination treatments without affecting algal cells. For this, agricultural fungicides (hexaconazole and copper oxychloride) have been tested at several concentrations and compared with a green decontamination strategy using calcium chloride biomineralization. Hexaconazole caused microalgae bleaching and chytrids bursting even at the lowest tested concentrations (0.01 ppm). At high concentrations, copper-oxychloride, was toxic for <i>H. pluvialis</i> cells, while the chytrids remained resistant to all concentrations of this fungicide. The cell number increased from 1 × 10⁵ ± 100 to 13 × 10⁵ ± 270 cells/ml with 0.25 ppm copper-oxide chloride after 15 days, but later decreased. Treatment with 150 ppm CaCl₂ induced an increase of astaxanthin after 7 days. One week later, algal growth exceeded 22 × 10⁵ cells/ml and the astaxanthin concentration was 22 times higher than the control. After 30 days, the concentration was 3 times higher than the control. Therefore, CaCl₂ at 150 ppm can be used as effective and economical control agent for commercial production of astaxanthin from <i>H. pluvialis</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144644078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parapedobacter flavus sp. nov., isolated from padding of fermentation bed","authors":"Rui Wang,&nbsp;Yan-Yan Zheng,&nbsp;Zi-Xuan Liu,&nbsp;Xuan Zhang,&nbsp;You-Jun Liao,&nbsp;Ya-Jing Yu,&nbsp;Ai-Hua Li","doi":"10.1007/s10482-025-02124-6","DOIUrl":"10.1007/s10482-025-02124-6","url":null,"abstract":"<div><p>A Gram-stain-negative, aerobic, rod-shaped bacterium strain, designated as 4-7-4^T, was isolated from the padding of a fermentation bed. Phylogenetic analyses based on 16S rRNA gene indicated that it belonged to the genus <i>Parapedobacter</i> and exhibited the closest relationship to <i>P. soli</i> KCTC 12984^T, with a similarity of 96.03%. The average nucleotide identity and digital DNA–DNA hybridization between strain 4-7-4^T and <i>P. soli</i> KCTC 12984^T were 78.6% and 21.6% respectively, both of which were below the thresholds for delineating species, supporting it being a novel species of <i>Parapedobacter</i>. The major polar lipids included phosphatidylethanolamine, unidentified aminolipid and four unidentified lipids. The predominant respiratory quinone was MK-7. The major cellular fatty acids were summed feature 3 (C_16:1 <i>ω</i>7<i>c</i> and/or C_16:1 <i>ω</i>6<i>c</i>) and iso-C_15:0. The genome of strain 4-7-4^T was 4890729 bp, and the genomic DNA G + C content was 49.8%. In summary, strain 4-7-4^T was identified as a novel species of <i>Parapedobacter</i>, for which the name <i>Parapedobacter flavus</i> sp. nov. was proposed. The type strain was 4-7-4^T (=CGMCC1.10938^T = KCTC 92351^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Stieleria mannarensis sp. nov., isolated from a marine sponge, and proposal to include members of the genus Roseiconus in the genus Stieleria","authors":"Nicolai Kallscheuer,&nbsp;Gaurav Kumar,&nbsp;Shabbir Ahamad,&nbsp;Sandhya Duddeda,&nbsp;Chintalapati Sasikala,&nbsp;Christian Jogler,&nbsp;Chintalapati Venkata Ramana","doi":"10.1007/s10482-025-02106-8","DOIUrl":"10.1007/s10482-025-02106-8","url":null,"abstract":"<div><p>A pink-coloured, salt- and alkali-tolerant strain, JC639^T, was isolated from a specimen of the marine sponge <i>Spheciospongia</i> sp. The novel isolate is motile, aerobic and forms oval to pear-shaped cells. It shares the highest 16S rRNA gene sequence identity with <i>Stieleria neptunia</i> Enr13^T and <i>Stieleria maiorica</i> Mal15^T in the family <i>Pirellulaceae</i> (phylum <i>Planctomycetota</i>). Optimum pH and temperature for growth are 8.0 (range 7.0–10.0) and 25 °C (range 10–30 °C), respectively. Major fatty acids are C_15:1ω8c, C_16:0, C_18:1ω9c, C_18:0, and C_16:1ω7c/C_16:1ω6c. Major polar lipids are phosphatidylcholine and phosphatidylethanolamine. The genome of strain JC639^T has a size of 9.56 Mb and a DNA G + C content of 59.5%. The polyphasic analysis of the isolate supports its delineation from the known species in the genus <i>Stieleria</i>. We therefore introduce <i>Stieleria mannarensis</i> sp. nov., represented by JC639^T (= KCTC 72168^T = NBRC 113878^T) as the type strain. Based on an analysis of established phylogenomic markers performed with all current members of the overlapping genera <i>Stieleria</i> and <i>Roseiconus</i>, we further propose to include the two so far characterized <i>Roseiconus</i> species in the genus <i>Stieleria</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the impact of HIS-1 and HSP-70 genes on drug response and pathology of Leishmania major using antisense oligonucleotides","authors":"Fatemeh Javani Jouni,&nbsp;Jaber Zafari,&nbsp;Hossein Vazini,&nbsp;Bahman Rahimi Esboei","doi":"10.1007/s10482-025-02113-9","DOIUrl":"10.1007/s10482-025-02113-9","url":null,"abstract":"<div><p>Leishmaniasis is a protozoan disease caused by the protozoan <i>Leishmania</i> spp. and presents a wide spectrum of manifestations. Researchers are currently unable to control and treat the different forms of leishmaniasis, and effective vaccines and treatments are not yet available. This study aimed to assess the pathological aspects and drug susceptibility of <i>L. major</i> treated with antisense oligonucleotides (ASO) targeting the HSP-70 and HIS-1 genes. This study investigates the anti-leishmanial properties of antisense oligonucleotides (ASO) directed against the HSP-70 and HIS-1 genes, which play critical roles in stage differentiation and resistance to anti-leishmanial drugs. The experiments were conducted using <i>L. major</i> promastigotes and amastigotes in vitro and a mouse model for cutaneous leishmaniasis (CL). Our results indicated that the expression of the HSP-70 and HIS-1 genes was significantly reduced in the group receiving antisense oligonucleotides (ASO). In the group treated with HSP-70 ASO, the amastigote count in macrophages was significantly reduced compared to the other groups. The findings from the in vivo experiments revealed that in the group receiving HIS-1 ASO, the size of the CL lesion was significantly reduced, and susceptibility to glucantime was significantly increased compared to the other groups. The results of the current study demonstrated that HIS-1 and HSP-70 ASOs successfully inhibit the pathogenicity of the L. major and decrease the lesion size of CL. ASO as a novel technique in genetic manipulation also revealed that it can aid in the control and prevention of various diseases.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disabling iron uptake and pilus assembly in uropathogenic Escherichia coli using CRISPR-Cas9: a step towards antivirulence therapy","authors":"Linu Thomas,&nbsp;Tajo Abraham","doi":"10.1007/s10482-025-02127-3","DOIUrl":"10.1007/s10482-025-02127-3","url":null,"abstract":"<div><p>Uropathogenic <i>Escherichia coli</i> (UPEC) is the leading cause of urinary tract infections (UTIs), driven by virulence factors such as iron acquisition systems and adhesive pili. In this study, we employed CRISPR-Cas9-mediated genome editing to functionally inactivate two critical virulence genes—<i>iucD</i>, involved in aerobactin-mediated iron uptake, and <i>papC</i>, encoding the outer membrane usher protein essential for P pilus assembly. Using a clinical UPEC isolate, we introduced premature stop codons via homologous repair templates guided by gene-specific single-guide RNAs. Colony PCR and Sanger sequencing confirmed precise site-specific editing, leading to truncated protein variants. In silico analyses using InterPro and Swiss-Model revealed a complete loss of essential domains in both proteins. Molecular docking studies demonstrated a marked reduction in binding affinities of truncated iucD for NAD(P)H and impaired protein–protein interaction between truncated PapC and PapG. This study highlights the utility of CRISPR-Cas9 as a powerful tool for dissecting bacterial pathogenesis and supports the potential of targeting virulence determinants like <i>iucD</i> and <i>papC</i> as part of an antivirulence strategy for managing UPEC infections.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic insights into slyA-mediated regulation of motility and secretion in Escherichia coli","authors":"Supraja Mohan,&nbsp;Prasanna Kumar Selvam,&nbsp;Karthick Vasudevan","doi":"10.1007/s10482-025-02119-3","DOIUrl":"10.1007/s10482-025-02119-3","url":null,"abstract":"<div><p><i>slyA</i>, a member of the MarR family of transcriptional regulators, has been implicated in regulating virulence, stress response, and motility in various Enterobacteriaceae. In this study, we performed transcriptomic profiling of <i>Escherichia coli</i> BW25113 wild-type and its isogenic <i>slyA</i> knockout strain using publicly available RNA-Seq data, each consisting of four biological replicates. Differential gene expression analysis identified 925 significantly altered genes, including key components of the flagellar assembly pathway, such as <i>fliA</i>, <i>flhB</i>, and <i>flgM</i>. Gene Ontology and enrichment analyses revealed significant modulation of biological processes, including bacterial motility, chemotaxis, and protein secretion. Network analysis further highlighted a subset of hub genes involved in motility and secretion, suggesting their centrality in <i>slyA</i>-mediated regulation. While the results indicate <i>slyA’</i>s influence on multiple cellular pathways, these observations may reflect both direct and indirect regulatory effects. This transcriptomic analysis provides a computational foundation for future mechanistic studies and highlights pathways that may be targeted for antimicrobial development.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome sequence analysis of pigmented Bacillus inaquosorum LBA001 revealed biosynthetic gene clusters for a variety of secondary metabolites","authors":"Temidayo Oluyomi Elufisan,&nbsp;Ninfa M. Rosas-García","doi":"10.1007/s10482-025-02122-8","DOIUrl":"10.1007/s10482-025-02122-8","url":null,"abstract":"<div><p>The strain <i>Bacillus inaquosorum</i> LBA001 was isolated from a soil sample as a potential entomopathogenic bacteria; and produces a brown pigment at each growth on LB (Luria–Bertani) agar. We investigated its biochemical and physiological characteristics using the standard bacteriological technique. The whole genome sequencing was used to explore its genetic composition and metabolic capabilities. We used whole genome sequencing to examine the role of its genetic makeup and its potential metabolic capacity. The whole genome assembly resulted in one scaffold with a GC content of 43.19% and a genome size of about 4.3 Mb. The Prokka annotation showed 4,149 coding sequences and full sets of ribosomal RNA (5S, 16S, and 23S). The pan genome analysis identified 192 core genes among the strains compared with numerous accessory genes in individual strains. Phylogenetic analysis confirmed that it belongs to the <i>B. inaquosorum</i> clade, as it has much in common with <i>B. inaquosorum</i> KCTC 13249^T. AntiSMASH indicated that LBA001 contains certain groups of biosynthetic genes such as bacilysin, surfactin, fengycin, bacillaene, and pulcherriminic acid among others. The predicted proteome of LBA001 was subjected to BLAST-based homology searches against reference pigment gene database. The analysis identified 23 high-confidence pigment biosynthesis genes, with five different types of pigments in seven distinct colors. Numerous CAZymes in LBA00 were predicted, suggesting its potential applications in biotechnology and agriculture. The identification of potential antibiotic resistance genes in LBA001 genome reflected its adaptive characteristics. These findings will add to our understanding of <i>B. inaquosorum</i> metabolic versatility and its potential as a beneficial source of bioactive compounds.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chryseobacterium tongliaoense sp. nov., an aniline blue-degrading strain isolated from agricultural soil","authors":"Ying Zhou,&nbsp;Lu Xu,&nbsp;Jiale Ma,&nbsp;Yu Lv,&nbsp;Qi Zhang,&nbsp;Jiandong Jiang,&nbsp;Jiguo Qiu,&nbsp;Zongzhuan Shen,&nbsp;Qirong Shen","doi":"10.1007/s10482-025-02121-9","DOIUrl":"10.1007/s10482-025-02121-9","url":null,"abstract":"<div><p>Aniline blue (AB) is a toxic dye with a wide range of applications. Although bacteria can degrade AB, their biodiversity remains limited. In this study, strain MD-1^T, capable of degrading AB, was screened from corn field soil obtained in Tongliao city in Inner Mongolia Autonomous Region, China. Strain MD-1^T degraded 81.7% of AB (100 mg·L⁻¹) within 72 h. Cells were aerobic, short rod, non-spore-forming and non-motile. The strain grew optimally at 30 °C, 1% (w/v) NaCl and pH 7.0. Phylogenetic trees of 16S rRNA gene sequences indicated that strain MD-1^T was classified in the genus <i>Chryseobacterium</i>, and shared 95.9%-96.8% similarities with type strains of the genus <i>Chryseobacterium</i>. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain MD-1^T and the related <i>Chryseobacterium</i> type strains were in the range of 20.9–41.2% and 76.9–90.3%, respectively. The fatty acid profile was mainly composed of C_15:0 iso, summed feature 9 (C_17:1 iso <i>ω</i>9c and/or C_16:0 10-methyl), C_17:0 iso 3OH and summed feature 3 (C_16:1 <i>ω</i>7c and/or C_16:1 <i>ω</i>6c), the major polar lipids were phosphatidylethanolamine (PE), three unidentified aminolipids (AL1-3), an unidentified aminophospholipid (APL) and seven unidentified lipids (L1-7). The only isoprenoid quinone was MK-6. The size of the genome of strain MD-1^T was 4.5 Mb with a G + C content of 36.5 mol%. Based on the low 16S rRNA gene sequence similarities, low dDDH and ANI values and differential phenotypic characteristics, we hypothesized that strain MD-1^T represents a novel species in the genus <i>Chryseobacterium</i>, for which the name <i>Chryseobacterium tongliaoense</i> sp. nov is proposed. The type strain is MD-1^T (= MCCC 1K09437^T = KCTC 102309^T). This study provided new microbial resource potential for remediation of AB pollution sustainably and efficiently.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robertkochia aurantiaca sp. nov., and Robertkochia flava sp. nov., two potential quinate- and catechol-degrading bacteria isolated from tidal-flat sediments","authors":"Jaincy N. Jayan,&nbsp;Young-Mi Lee,&nbsp;Ye-ji You,&nbsp;Sathiyaraj Srinivasan,&nbsp;Sang-Seob Lee","doi":"10.1007/s10482-025-02102-y","DOIUrl":"10.1007/s10482-025-02102-y","url":null,"abstract":"<div><p>Two Gram-negative-strains, 3YJGBD-33^T and 3YJGBD-35^T were isolated from tidal flat sediment in Daebudo, Republic of Korea. The isolates are non-motile, and long rods. Both strains are oxidase-positive and grew in the range of 15–45 °C (optimum, 40 °C) and pH 7–8 (optimum pH 8.0). Growth was observed in the salinity range of 1–6%, with optimum growth at 4.0% for strain 3YJGBD-33^T and 3.0% for strain 3YJGBD-35^T. According to the NCBI 16S ribosomal RNA sequences database the strain 3YJGBD-33^T and 3YJGBD-35^T showed the highest 16S rRNA gene similarity with <i>Robertkochia marina</i> CC-AMO-30D^T (97.0% and 98.4%), <i>Robertkochia sediminum</i> 1368^T (96.4% and 98.3%) and <i>Robertkochia solimangrovi</i> CL23^T (96.0% and 96.5%). The major respiratory quinone is MK-6. The polar lipid profile consists of phosphatidylethanolamine, three aminolipids and two unidentified lipids. The major cellular fatty acids for strains 3YJGBD-33^T and 3YJGBD-35^T were iso-C_15:0, iso-C_15:1 G and iso-C_17:0 3-OH. The draft genome of strains 3YJGBD-33^T and 3YJGBD-35^T are 3.4 Mbp and 3.5 Mbp in size with 2993 coding genes, 41 tRNAs and 2935 coding genes, 42 tRNAs, respectively. The polyphasic analysis confirms that strains 3YJGBD-33^T and 3YJGBD-35^T belong to the genus <i>Robertkochia</i> and represent novel species, for which the names <i>Robertkochia aurantiaca</i> sp. nov. and <i>Robertkochia flava</i> sp. nov. are proposed. The type strains are 3YJGBD-33^T (= KCTC 82715^T = TBRC 19393^T) and 3YJGBD-35^T (= KCTC 82716^T = JCM 34784^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144576876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}