Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Biocontrol potential of four Pseudomonas strains against tobacco black shank disease","authors":"Mingya Zhang,&nbsp;Quan Ren,&nbsp;Xiuqing Wu,&nbsp;Meng Liu,&nbsp;Lin Zhang,&nbsp;Qiong Yuan,&nbsp;Ning Lu,&nbsp;Yi Cao","doi":"10.1007/s10482-025-02092-x","DOIUrl":"10.1007/s10482-025-02092-x","url":null,"abstract":"<div><p>Tobacco black shank is a significant disease that poses a severe threat to tobacco production. To identify effective biocontrol agents for combating tobacco black shank disease, a screening of 39 putative <i>Pseudomonas</i> strains was conducted utilizing the plate confrontation assay. Four strains exhibiting inhibitory effects were selected and further evaluated for their disease control potential in pot trials. The effects of the strains on the growth of tobacco seedlings were detected using the root-irrigation method. The results demonstrated that four <i>Pseudomonas</i> strains exhibited significant inhibitory effects against tobacco black shank disease. The inhibition rates of strains YC2140, YC2090, YC2202, and Tr157 against <i>P. nicotianae</i> were determined to be 77.20%, 73.43%, 73.07%, and 32.46%, respectively. Pot trials showed that the biocontrol efficiency of Tr157, YC2090, YC2140, YC2202 against tobacco black shank disease was 96.30%, 66.67%, 62.96%, 62.96%, respectively. In addition, the strain Tr157 was found to be capable of producing indole-3-acetic acid (IAA). Meanwhile, Tr157, YC2140, and YC2202 demonstrated the ability to solubilize phosphorus, while all the four strains were capable of solubilizing potassium and producing siderophores. These strains also promoted tobacco growth increasing the total fresh weight by 146.33%, 153.21%, 87.16% and 239.91%, respectively. Overall, this study underscores the potential of the four <i>Pseudomonas</i> strains as effective biocontrol agents against tobacco black shank disease, offering an eco-friendly and sustainable alternative to chemical fungicides in agriculture.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic insights into Neopusillimonas aestuarii sp. nov., a novel estuarine bacterium with adaptations for ectoine biosynthesis and stress tolerance","authors":"Kiran Kirdat,&nbsp;Umera Patwekar,&nbsp;Kunal Jain,&nbsp;Malad Mubarak,&nbsp;Pradeep Choudhary,&nbsp;Lucky Thakkar,&nbsp;Vipool Thorat,&nbsp;Datta Madamwar,&nbsp;Tushar Lodha,&nbsp;Amit Yadav","doi":"10.1007/s10482-025-02093-w","DOIUrl":"10.1007/s10482-025-02093-w","url":null,"abstract":"<div><p>A novel Gram-stain-negative, aerobic rod-shaped bacterial strain, which was catalase- and oxidase-positive, designated as DMV24BSW_D^T, was isolated from the estuarine waters of the Bhavnagar (India) coast of the Arabian Sea. Its 16S rRNA gene exhibited 99.52% similarity with <i>Neopusillimonas maritima</i> 17-4A^T, followed by 97.95% similarity with <i>the Pusillimonas caeni</i> strain EBR-8–1 and 97.4% similarity with <i>the P. noertemannii</i> strain BN9^T. Phylogenomic analysis using BPGA (14,332 aa) and UBCG (90,261 bp) tools revealed a unique phylogenetic position within the genus <i>Neopusillimonas</i>. The genome exhibited a G + C content of 53.25%. In comparison with <i>N. maritima</i> 17-4A^T, the strain demonstrated an average nucleotide identity (ANIb) of 94.47% and a digital DNA-DNA hybridization (dDDH) value of 60.1%, indicating distinct genomic divergence. The genome of DMV24BSW_D^T contains several unique metabolic genes that facilitate efficient electron transfer during aerobic respiration. Additionally, it harbours one intact prophage and four defective prophages, indicating ongoing viral interactions. The genome encodes a complete pathway for ectoine biosynthesis and transportation. Strain DMV24BSW_D^T tested positive for gelatin hydrolysis and demonstrated the ability to utilize a wide range of carbohydrates, including α-D-glucose, D-melibiose, D-fructose, L-rhamnose, and various organic acids, such as methyl pyruvate and propionic acid, along with tolerance to fluctuating pH (5 to 10) and salinity (0–4% NaCl). The major polar lipids included phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine, while fatty acid analysis revealed C_12:0, C_16:0, C_17:0 cyclo, and summed feature 2 (C_12:0 aldehyde/unknown) as major components. The respiratory quinones identified were MK-7 and MK-8. These comprehensive phenotypic, chemotaxonomic, and genomic characteristics support the unique taxonomic position of DMV24BSW_D^T within the genus <i>Neopusillimonas</i> and the proposal of a novel species of the genus Neopusillimonas, for which the name <i>Neopusillimonas aestuarii</i> sp. nov. (Type strain DMV24BSW_D^T = MCC 2506^T = KCTC 72453^T = JCM 34508^T) is proposed.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-025-02093-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, antibacterial susceptibility, and virulence gene profiles of Arcobacter species isolated from seafood","authors":"Ahmad Jazini,&nbsp;Ebrahim Rahimi,&nbsp;Amir Shakerian,&nbsp;Faham Khamsipour","doi":"10.1007/s10482-025-02091-y","DOIUrl":"10.1007/s10482-025-02091-y","url":null,"abstract":"<div><p><i>Arcobacter butzleri</i>, an emerging foodborne zoonotic pathogen, is increasingly associated with gastrointestinal and systemic infections in humans, and with diarrhea, mastitis, and abortions in animals. This study aimed to determine the prevalence, antimicrobial susceptibility, and virulence gene profiles of <i>Arcobacter</i> species isolated from seafood. A total of 560 samples were analyzed, including 300 fresh seafood samples (100 fresh/raw fish, 50 shrimp, 50 crabs, 50 oysters, and 50 lobsters) and 260 preserved seafood samples (70 smoked fish, 70 salted fish, 50 dried fish, and 70 frozen fish). <i>Arcobacter</i> spp. were isolated using an <i>Arcobacter</i> an enrichment and direct filtration method. Antibiotic susceptibility was assessed using the disk diffusion method following Clinical and Laboratory Standards Institute guidelines. PCR was conducted to detect ten putative virulence genes and five resistance genes. <i>Arcobacter</i> spp. were detected in 21.43% (120/560) of the tested seafood samples, with the highest prevalence in fresh fish (42%) and oysters (40%). Among isolates, <i>A. butzleri</i> was predominant (14.82%), followed by <i>A. cryaerophilus</i> (6.25%) and <i>A. skirrowii</i> (0.36%). Virulence genes were present in 84.6% of <i>A. butzleri</i> and 73.5% of <i>A. cryaerophilus</i> isolates. Resistance genes were found in 82.7% and 67.6% of these species, respectively. Antibiotic resistance was highest in <i>A. butzleri</i>, particularly to vancomycin, cephalosporins, ciprofloxacin, and beta-lactams, while <i>A. skirrowii</i> showed minimal resistance. Most isolates remained sensitive to polymyxin-B, colistin, gentamicin, and carbapenems. The study finds high rates of contamination of fresh fish, smoked fish, salted fish, dried fish, frozen fish, oysters, crabs, lobster, and shrimp, indicating the potential role of <i>Arcobacter</i> spp. in causing infections.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering the burden of tuberculosis in Algeria: a retrospective analysis from 2019 to 2024","authors":"Amina Zergui,&nbsp;Nacim Bouziani,&nbsp;Kawtar Fidjel,&nbsp;Choubaila Leksir,&nbsp;Yamina Merzouk,&nbsp;Fatima Hanane Chentouf,&nbsp;Imane Djaballi,&nbsp;Mebrouk Kihal","doi":"10.1007/s10482-025-02096-7","DOIUrl":"10.1007/s10482-025-02096-7","url":null,"abstract":"<div><p>Tuberculosis (TB) remains a global health challenge, particularly in low- and middle-income countries. This retrospective study aimed to analyze the epidemiological patterns, clinical characteristics, and treatment outcomes of TB in Algeria. A total of 729 medical records from patients treated at a public health facility in Oran between January 2019 and June 2024 were reviewed. The study revealed a mean patient age of 35.7 years (SD ± 16.4), with cases ranging from 1 to 88 years. Males accounted for 55% of TB cases, maintaining a consistent predominance across the study period. TB manifested primarily in two forms: pulmonary TB (PTB) in 45% of cases and extrapulmonary TB (EPTB) in 50%, with a small proportion (5%) presenting both types. EPTB was more prevalent among females (55%) and pediatric patients, whereas PTB was more frequent in individuals over 40 years. Comorbidities were documented in 19% of patients, with diabetes (7.5%), hypertension (6.2%), and HIV (1.8%) being the most common, predominantly affecting those over 50 years. Diagnosis relied on microscopy (52%), radiology (45%), and culture (3%). Most patients (83%) were newly diagnosed, while 17% had relapsed. Treatment involved Category I (83%) and Category II (17%) regimens. Overall, treatment outcomes were favorable, with a 91% cure rate, 8% drug resistance, and mortality below 1%. Between 2019 and 2023, cure rates improved from 89 to 94%, while resistance declined from 10 to 6%. These findings provide valuable insights into TB trends in Algeria, guiding public health interventions for better disease control.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety aspects and in vitro probiotic assessment of Kluyveromyces marxianus strains from neonatal faeces","authors":"Hacer Meral-Aktaş,&nbsp;Bülent Çetin,&nbsp;Muhammet Akif Güler,&nbsp;Bülent Albayrak,&nbsp;Kadir Şerafettin Tekgündüz,&nbsp;Mustafa Kara,&nbsp;Ali Işlek","doi":"10.1007/s10482-025-02090-z","DOIUrl":"10.1007/s10482-025-02090-z","url":null,"abstract":"<div><p>The isolation and identification of probiotic yeasts is increasing rapidly. In this context, the present study aimed to isolate and identify yeast strains from neonatal faeces in Erzurum province, Türkiye and to determine their probiotic characteristics. A total of 12 yeast strains were isolated and genotypic characterization revealed the presence of seven different species, including <i>Kluyveromyces marxianus</i>, <i>Candida</i> spp. <i>Clavispora lusitaniae, Geotrichum candidum, Trichophyton rubrum, Pichia cactophila,</i> and <i>Meyerozyma guilliermondii</i>. The non-pathogenic and potentially probiotic characteristics of the <i>K. marxianus</i> M2, M9, and M10 strains were further investigated. Although yeast has been isolated from neonatal faeces before, <i>K. marxianus</i> was isolated for the first time in this study. The results revealed that the <i>K. marxianus</i> strains exhibited high resistance to simulated gastric juice and bile salts. The auto-aggregation percentages of the strains ranged from 92.55 to 94.78% after 4 h, while the co-aggregation percentages with pathogens ranged from 19.70 to 53.09%. The <i>K. marxianus</i> M2 strain exhibited the highest degree of hydrophobicity (74.97%), and none of the strains demonstrated DN-ase or haemolytic activity. Furthermore, M2 and M9 strains displayed bile salt hydrolase activity. In conclusion, based on in vitro probiotic test results, <i>K. marxianus</i> strains were selected as probiotic yeast candidates for further studies, especially in patients under antibiotic therapy.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144073850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, biochemical, and molecular characterization of the root-knot nematode Meloidogyne spartinae and assessment of bacterial assemblages in infected smooth cordgrass roots","authors":"Janete A. Brito,&nbsp;Samuel J. Martins,&nbsp;Amy Michaud,&nbsp;Sergei A. Subbotin,&nbsp;Silvia Vau,&nbsp;Josephine M. Pasche,&nbsp;Ruimin Xue,&nbsp;Jason D. Stanley","doi":"10.1007/s10482-025-02089-6","DOIUrl":"10.1007/s10482-025-02089-6","url":null,"abstract":"<div><p>Smooth cordgrass (<i>Spartina alterniflora</i>), a keystone species in marine ecosystems is crucial for maintaining the health and stability of coastal habitats. However, the cordgrass root-knot nematode (<i>Meloidogyne spartinae</i>), has been reported infecting this plant and still very little is known about this plant-parasitic nematode species well adapted to coastal saltmarsh. Morphological, biochemical, molecular, and gall microbiome analyses were performed using three <i>M. spartinae</i> populations to better characterized this nematode. <i>M. spartinae</i> females did not produce egg masses; females, eggs and males are found inside of the root galls. No esterase activity was detected using homogenates of one, five or ten females of <i>M. spartinae</i> per well regardless of the population. Nevertheless, one strong band of malate dehydrogenase activity (MDH = N1b phenotype) was detected using macerate of one female. Partial 18S rRNA, the D2-D3 expansion segments of 28S rRNA, partial <i>COI</i> and <i>NAD5</i> gene sequences were obtained<i>.</i> The phylogenetical analyses showed that <i>M. spartinae</i> was related to <i>M. marylandi</i> and <i>M. graminis</i>. Microbiome analysis of cordgrass root galls revealed Proteobacteria as the most abundant phylum and <i>Vibrio</i> as the most abundant genus, in addition other environmentally important bacterial genera, such as those with nitrogen fixation and sulfate reducing roles. Our findings provide novel information on the bacterial assemblages of cordgrass infected roots and demonstrated via rRNA and mtDNA gene sequences that <i>M. spartinae</i> is clearly different from all other <i>Meloidogyne</i> spp.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defining the biomarkers in anti-MRSA fractions of soil Streptomycetes by multivariate analysis","authors":"Mohamed Sebak,&nbsp;Venugopal Mukku,&nbsp;Amal E. Saafan,&nbsp;Walid Bakeer,&nbsp;Sameh Abd-ELGhani,&nbsp;Ahmed O. El-Gendy,&nbsp;RuAngelie Edrada-Ebel","doi":"10.1007/s10482-025-02087-8","DOIUrl":"10.1007/s10482-025-02087-8","url":null,"abstract":"<div><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is one of the most alarming antibiotic-resistant pathogens causing nosocomial and community-acquired infections. Actinomycetes, particularly <i>Streptomycetes</i>, have historically been a major source of natural products including anti-MRSA agents. Hence, we designed this study to isolate new anti-MRSA natural products from <i>Streptomycetes</i> aided by liquid chromatography-electrospray ionization-high resolution mass spectrometry (LC-ESI-HRMS)-based metabolomics data analysis and dereplication study to select the best fractions for isolation work. Following this approach, two new natural products, namely, (4-propylphenyl) methane-diazonium (<b>1</b>) and (4<i>E</i>)-6-oxooctadec-4-enoic acid (<b>2</b>) and three known compounds, 8-methyl-5-oxononanoic acid (<b>4</b>), 15-methylhexadecanoic acid (<b>5</b>), and (<i>Z</i>)-14-methylpentadecenoic acid (<b>6</b>) were isolated from two soil <i>Streptomyces</i> sp. Additionally, (9<i>E</i>)-11-hydroxyoctadec-9-enoic acid (<b>3</b>) was recovered for the first time from <i>Streptomycetes</i>. The minimum inhibitory concentration (MIC) of compounds <b>1</b>,<b> 5</b>, and<b> 6</b> against MRSA were 128, 64, and 64 µg/mL respectively. The compounds were slightly more active against <i>B</i>. <i>subtilis</i> with MIC values of 64, 64, and 32 µg/mL, respectively. Our research adds to the growing knowledge about the importance of combining sophisticated analytical methods in the chemical profiling of the fractions with the outcomes of the bioassays throughout the subsequent fractionation processes to help isolate new secondary metabolites from natural sources.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of Exiguobacterium abrahamii sp. nov., a novel strain isolated from mangrove soil with lanthipeptide biosynthetic genes","authors":"Sajna Salim,&nbsp;Shiburaj Sugathan","doi":"10.1007/s10482-025-02079-8","DOIUrl":"10.1007/s10482-025-02079-8","url":null,"abstract":"<div><p><i>Exiguobacterium</i> species are known for their adaptability to extreme environments, making them valuable candidates for biotechnological applications. This study aimed to characterise a novel <i>Exiguobacterium</i> isolate, designated TBG-PICH-001^T, isolated from the Pichavaram estuaries in Tamil Nadu, India, and to determine its taxonomic position within the genus. A polyphasic approach combining phenotypic, genotypic and chemotaxonomic was employed to characterise the isolate TBG-PICH-001. Morphological and biochemical characterisation revealed that the strain is a Gram-positive, motile, rod-shaped, non-spore-forming bacterium that exhibited a characteristic yellow pigmentation. Whole genome sequencing and annotation determined the G + C content to be 47%. Phylogenetic analysis based on 16S rRNA gene positioned that strain TBG-PICH-001^T is closely related to <i>E. acetylicum</i> strain DSM 20416 (NR_043479.1:1–1550), <i>E. indicum</i> strain HHS31 (NR_042347.1:1–1502), and <i>E. enclense</i> strain NIO-1109 (NR_178487.1:1–1415), while the digital DNA-DNA hybridisation (dDDH) confirmed that the organism belongs to the <i>Exiguobacterium</i> genus. Fatty acid analysis showed that the major fatty acid was C16.0 (30.95%), followed by iso-C15.0 (12.87%), iso-C13.0 (11.91%), C18.0 (11.06%), anteiso-C13.0 (10.39%), iso-C17.0 (8.55%), anteiso-C15.0 (3.22%), and C14.0 (2.49%). The strain shows resistance to cold temperatures and high salt concentrations. Genomic analysis revealed the presence of stress response genes and transporters, suggesting that the strain is well adapted to survive in harsh environments. AntiSMASH analysis of the genome showed the presence of lantipeptide biosynthetic genes, suggesting the strain's potential to produce antimicrobial compounds. Biochemical, genotypic, and chemotaxonomic characterisation strongly supports the strain TBG-PICH-001^T as a novel species within the <i>Exiguobacterium</i> genus and named <i>Exiguobacterium abrahamii</i> sp. nov. This study enhances the understanding of <i>Exiguobacterium</i> diversity and its adaptability to diverse environments, opening a new avenue for biotechnology exploration.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chicken and pig manures application on heavy metal in different soil types and on bacterial in the rhizosphere soil of Chinese cabbage","authors":"Lili Zhang,&nbsp;Ke Liu,&nbsp;Shiqing Feng,&nbsp;Fan Yang,&nbsp;Ren He,&nbsp;Nannan Jing,&nbsp;Huiying Wu","doi":"10.1007/s10482-025-02085-w","DOIUrl":"10.1007/s10482-025-02085-w","url":null,"abstract":"<div><p>In China, due to the rapid growth of the livestock and poultry farming industries, large amounts of manure are produced every year, which contains large amounts of heavy metals, and field application of this manure as fertilizer leads to the accumulation of heavy metals (HMs) in soils. In this study, a pot experiment and 16S rRNA gene sequencing were used to study the soil chemical properties and bacterial community characteristics of the Chinese cabbage rhizosphere in purple soil, red soil and yellow soil under fertilizer (CF), chicken manure (CM) and pig manure (PM) application. Compared with the CK and CF, chicken manure (CM3 and CM6) and pig manure (PM3 and PM6) application significantly increased the soil pH, soil organic matter (SOM), Cu and Zn contents, with greater increases at higher application rates. There were no significant changes in the total and available Cd and Pb contents in purple soil, but the contents of these heavy metals significantly decreased in red soil and yellow soils. The application of fertilizer increased the Cu, Zn, Cd, and Pb contents in the shoots of Chinese cabbage, while the trends of heavy metals contents differed by soil type and manure type under other treatments. PERMANOVA revealed that the application of chicken manure and pig manure significantly affected the bacterial community structure of the cabbage rhizosphere soil regardless of soil type (P = 0.001). Network analysis revealed that application of chemical fertilizers enhanced network complexity, whereas the opposite was true for application of chicken and pig manure, and the relative abundance of Proteobacteria increased by 1.69% ~ 6.21%, 2.41% ~ 5.41% and 5.65% ~ 12.12%, respectively. Redundancy analysis (RDA) revealed that pH, SOM and Zn were the main factors affecting the bacterial communities in the rhizosphere soil. The response of rhizosphere bacteria to different soil types differed. A correlation heatmap revealed that <i>Lysobacter</i> and <i>Sphingomonas</i> were heavy metal-resistant strains. The effects of Cu and Zn on bacteria in different rhizosphere soils were quite different. This study provides a reference for the safe application of livestock manure to agricultural fields, and the results suggest that heavy metal-resistant strains can be used to assist in the remediation of HM-polluted environments.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paracoccus yibinensis sp. nov., a novel bacterium with astaxanthin producing isolated from the environment of Chinese distilled Baijiu","authors":"Qingchun Luo,&nbsp;Yanping Lu,&nbsp;Xi Li,&nbsp;Jian Su,&nbsp;Pengju Zhao,&nbsp;Zhu Zhang,&nbsp;Yang Gu,&nbsp;Dong Zhao,&nbsp;Jia Zheng","doi":"10.1007/s10482-025-02082-z","DOIUrl":"10.1007/s10482-025-02082-z","url":null,"abstract":"<div><p>An aerobic, Gram-stain-negative bacterium, designated as strain WLY502^T, was isolated from the environment of <i>Baobaoqu</i> starter production. The strain was with a short rod shape cells 0.5–0.7 μm wide and 1.1–2.0 μm long, arranged singly or in pairs. The isolate was able to grow at temperatures of 20–37 °C (optimum growth at 25 °C), at pH 5.0–10.0 (optimum growth at pH 7.0) and in the presence of 0–5% (w/v) NaCl (optimum growth with 0% NaCl). Ribose, xylose, arabinose, mannose, glucose and galactose were the major cell-wall sugar. The <i>meso</i>-diaminopimelic acid (<i>meso</i>-DAP) was the diagnostic amino acid. The main polar lipids of strain WLY502^T included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), unidentified aminolipids (UAL 1–2), unidentified aminophospholipid (UAPL), unidentified aminophospholipid (UAPL), unidentified phospholipid (UPL), unidentified lipids (UL 1–5), glycolipid (GL). Ubiquinone Q-10 was predominant menaquinone and C_18:1 ω7c (80.5%) was the major fatty acid. Comparisons of 16S rRNA gene sequence indicated that strain WLY502^T was most closely related to <i>Paracoccus acridae</i> SCU-M53^T (98.48%), <i>Paracoccus haematequi</i> M1-83^T (98.40%). The average nucleotide identity (ANI) values strain WLY502^T and the two type strains mentioned above were 91.19% and 70.96%. The average amino identity (AAI) value between them were 93.42% and 87.19%. Additionally, the digital DNA-DNA hybridization (dDDH) value between them is 42.9% and 30.1%. The genomic DNA G + C content was 64.99%. Strain WLY502^T could produce astaxanthin and contains several genes (such as MalK, UgpE, UgpA, PRK07067, PBP2 TMBP ike, YcjU, YjiC, crtI-fam, crtY, ERG9, and IspA) involved in astaxanthin. Therefore, strain WLY502^T may have development and utilisation value in flavouring agents. Based on the evidence presented, strain WLY502^T (CICC 25237^T = JCM 36429^T) is proposed as the type strain of a novel species, designated as <i>Paracoccus yibinensis sp. nov</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}