Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Comprehensive genomic and phenotypic characterization of thermophilic bacterium Sinimarinibacterium thermocellulolyticum sp. nov. HSW-8T, a cellulase-producing bacterium isolated from hot spring water in South Korea","authors":"Yue Jiang,&nbsp;Zhun Li","doi":"10.1007/s10482-024-02055-8","DOIUrl":"10.1007/s10482-024-02055-8","url":null,"abstract":"<div><p>A thermophilic cellulase-producing bacterium, strain HSW-8^T, isolated from hot spring waters in South Korea, was subjected to a taxonomic analysis. Cells of strain HSW-8^T were gram-stain-negative, facultatively anaerobic, rod-shaped, with optimum growth at 45 °C, pH 7.0, in the presence of 0% (w/v) NaCl. Strain HSW-8^T showed the highest 16S rRNA gene sequence similarity to <i>Sinimarinibacterium flocculans</i> NH6-24^T (97.52%), followed by <i>Fontimonas thermophila</i> DSM 23609^T (96.97%), <i>Solimonas flava</i> CW-KD 4^T (95.24%), and <i>Solimonas variicoloris</i> DSM 15731^T (95.18%). Based on 16S rRNA phylogeny, strain HSW-8^T is phylogenetically closely related to <i>Fontimonas thermophila</i> DSM 23609^T and <i>Sinimarinibacterium flocculans</i> DSM 104150^T and could be distinguished from the type species based on their phenotypic properties. The genome length of strain HSW-8^T was 3.32 Mbp with a 67.33% G + C content. The average nucleotide identity and digital DNA–DNA hybridization values between strain HSW-8^T and its closely related type strains were 75.4–83.2 and 20.2–26.2%, respectively. Summed feature 8 (C_18:1<i>ω</i>7c and/or C_18:1<i>ω</i>6c), C_16:0, and iso-C_16:0 identified the major fatty acids (&gt; 10%). Phosphatidylglycerol and phosphatidylethanolamine were demonstrated as the major polar lipids while the respiratory quinone is ubiquinone-8. Strain HSW-8^T exhibited multiple adaptations for survival at high temperatures, including diverse potential motility mechanisms and toxin-antitoxin systems, as evidenced by both phenotypic characteristics and genomic analysis. Based on genotypic and phenotypic features, strain HSW-8^T (= KCTC 92765^T = GDMCC 1.4313^T) represents a novel <i>Sinimarinibacterium</i> species, in which the name <i>Sinimarinibacterium thermocellulolyticum</i> sp. nov. is proposed.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142905970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max)","authors":"Peter Kämpfer,&nbsp;Stefanie P. Glaeser,&nbsp;John A. McInroy,&nbsp;Hans-Jürgen Busse,&nbsp;Dominique Clermont,&nbsp;Alexis Criscuolo","doi":"10.1007/s10482-024-02051-y","DOIUrl":"10.1007/s10482-024-02051-y","url":null,"abstract":"<div><p>A Gram-staining-positive, aerobic bacterium, designated strain JJ-181^T, was isolated from the root surface of soybean. Based on the 16S rRNA gene sequence similarities, strain JJ-181^T was grouped into the genus <i>Cohnella</i>, most closely related to <i>Cohnella hashimotonis</i> F6_2S_P_1^T (98.85%) and <i>C. ginsengisoli</i> GR21-5^T (98.3%). The pairwise average nucleotide identity and digital DNA-DNA hybridisation values of the JJ-181^T genome assembly against publicly available <i>Cohnella</i> type strain genomes were below 84% and 28%, respectively. The fatty acid profile from whole cell hydrolysates, the cell wall diaminoacid, the quinone system, the polar lipid profile, and the polyamine pattern supported the allocation of strain JJ-181^T to the genus <i>Cohnella</i>. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain JJ-181^T from its closely-related <i>Cohnella</i> species. Functional analysis revealed that strain JJ-181^T has different gene clusters related to swarming motility, chemotaxis ability, and endospore and biofilm formations. The gene content also suggests the ability of strain JJ-181^T to synthesise biotin and riboflavin, as well as indole-3-acetic acid, an important phytohormone for plant growth. Based on polyphasic analyses, strain JJ-181^T can be classified as a new species of the genus <i>Cohnella</i>, for which we propose the name <i>Cohnella rhizoplanae</i> sp. nov., with strain JJ-181^T (= LMG 31678^T = CIP 112018^T = CCM 9031^T = DSM 110650^T) as the type strain.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-024-02051-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel antibiotic resistance profiles in bacteria isolated from oil fly larvae Helaeomyia petrolei living in the La Brea Tar Pits","authors":"Lisa M. Durso,&nbsp;Md. Shamimuzzaman,&nbsp;Brian Dillard,&nbsp;Kenneth W. Nickerson","doi":"10.1007/s10482-024-02050-z","DOIUrl":"10.1007/s10482-024-02050-z","url":null,"abstract":"<div><p>Larvae from the petroleum oil fly, <i>Helaeomyia petrolei</i>, live in the asphaltene and polyaromatic hydrocarbon rich asphalt seeps of Rancho La Brea, Los Angeles, California. These larvae pass high amounts of viscous asphalt through their digestive system, and their gut microbiota is exposed to these extreme conditions. Environmental stress response mechanisms can co-select for antibiotic resistance, and in the current study we used 16S rRNA and genomic sequencing along with the Comprehensive Antibiotic Resistance Database (CARD) tools to characterize antibiotic resistance profiles from six bacteria previously isolated from the oil fly larval intestinal tract, linking phenotypic and genotypic resistance profiles. The isolates contain a core set of antibiotic resistance determinants along with determinants that are rarely found in these species. Comparing these oil fly isolates to the phenotypic prevalence data generated by the CARD Resistance Gene Identifier revealed sixteen instances where the oil fly bacteria appeared to carry a resistance not seen in related taxa in the database, suggesting a novel suite of resistance families in the oil fly isolates compared to other members of the same taxa. Results highlight the functional duality of genes that simultaneously code for antibiotic resistance and survival under extreme conditions, and expand our understanding of the ecological and evolutionary role of antibiotic resistance genes in environmental habitats.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-024-02050-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic and genomic characterization of a siderophore-producing bacterium, Rhodoligotrophos ferricapiens sp. nov isolated from lettuce cultivation soil","authors":"Yu-Jin Kim,&nbsp;Yong-Seok Kim,&nbsp;Chang-Jun Cha","doi":"10.1007/s10482-024-02052-x","DOIUrl":"10.1007/s10482-024-02052-x","url":null,"abstract":"<div><p>A Gram-stain-negative, aerobic, non-spore-forming, non-motile, coccus-shaped, and red-pigmented bacterial strain designated as CJ14^T was isolated from lettuce cultivation soil in Yong-In, South Korea. Strain CJ14^T grew optimally on Luria–Bertani agar at 37 ℃ and pH 7.0 in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CJ14^T belonged to the genus <i>Rhodoligotrophos</i> in the family <i>Rhodoligotrophaceae</i> and was closely related to <i>Rhodoligotrophos defluvii</i> lm1^T (98.4% similarity). The genome size of strain CJ14^T was 4.8 Mbp consisting of a single contig, and the DNA G + C content of strain CJ14^T was 61.8%. The genomic comparison of strain CJ14^T to <i>R. defluvii</i> lm1^T showed an average nucleotide identity value of 77.9%, and the digital DNA–DNA hybridization value was 22.4%. Genomic analyses revealed that strain CJ14^T possessed a gene cluster for ochrobactin biosynthesis, highly homologous to the siderophore gene cluster from <i>Nitratireductor thuwali</i> Nit1536^T. Strain CJ14^T contained ubiquinone (Q-10) as the predominant respiratory quinone. The major cellular fatty acids of strain CJ14^T were C_16:0, C_19:0 cyclo <i>ω</i>8<i>c</i> and summed feature 8. The polar lipid profile was composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, one unidentified aminolipid, one unidentified glycolipid and five unidentified lipids. Based on polyphasic taxonomy study, strain CJ14^T could be classified as a novel species of the genus <i>Rhodoligotrophos</i>, for which the name <i>Rhodoligotrophos ferricapiens</i> sp. nov. is proposed. The type strain is CJ14^T (= KACC 23063^T = JCM 36057^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial diversity and community structure of microalgae (Nannochloropsis oculata) and rotifer (Brachionus plicatilis) in aquaculture by 16S rRNA amplicon-based sequencing","authors":"Andrés Carranza-Quirós,&nbsp;Frank Solano-Campos,&nbsp;Milagro Carvajal-Oses,&nbsp;Jonathan Chacón-Guzmán,&nbsp;Paola Navarrete,&nbsp;Samira Reinoso,&nbsp;Rodolfo Umaña-Castro","doi":"10.1007/s10482-024-02053-w","DOIUrl":"10.1007/s10482-024-02053-w","url":null,"abstract":"<div><p>Live cultures, including <i>Nannochloropsis oculata</i> and <i>Brachionus plicatilis</i>, are essential in aquaculture due to its economic and nutritional value for commercial fish species. Pathogens and probiotics can be introduced to aquaculture systems by live feed, with variations in abundance influenced by environmental physicochemical parameters. To investigate this, amplicon sequencing of the V3-V4 region of the 16S rRNA was conducted using Illumina MiSeq to elucidate bacterial abundances and their variations in response to changes in physicochemical parameters in live feed cultures. Results revealed that pathogens like, <i>Tenacibaculum</i>, predominantly found in rotifer cultures, was inhibited under dissolved oxygen levels of 6.87–8.19 mg/L and pH levels of 8.63–9.23. <i>Winogradskyella</i>, mainly present on day 1 of the microalgae culture, thrived under these same conditions. Potential probiotics were also analyzed. <i>Phaeodactylibacter</i>, primarily found on day 1 of microalgae cultures, was favored by dissolved oxygen levels of 7.11–7.68 mg/L and pH levels of 8.63–9.23 but was inhibited by nitrite levels of 0.46–0.5 mg/L. Overall, dissolved oxygen was the key parameter modulating microbial diversity. This study shows that optimizing environmental parameters can enhance culture health by promoting probiotics and reducing harmful bacteria.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proposal of Crystallibacter gen. nov., Crystallibacter permensis sp. nov. and Crystallibacter degradans sp. nov. for the salt-tolerant and aromatics degrading actinobacteria, and reclassification of Arthrobacter crystallopoietes as Crystallibacter crystallifaciens comb. nov.","authors":"Elena G. Plotnikova,&nbsp;Elena V. Ariskina,&nbsp;Olga V. Yastrebova,&nbsp;Natalia V. Potekhina,&nbsp;Alexander N. Avtukh,&nbsp;Sergey V. Tarlachkov,&nbsp;Irina P. Starodumova,&nbsp;Lyubov V. Dorofeeva,&nbsp;Lyudmila I. Evtushenko","doi":"10.1007/s10482-024-02048-7","DOIUrl":"10.1007/s10482-024-02048-7","url":null,"abstract":"<div><p>Four salt-tolerant and aromatics degrading strains used in this study were isolated from polluted technogenic soil on the territory of the Verkhnekamsk potash deposit (Russia). The strains were aerobic, Gram-stain-positive, non-motile, non-endospore-forming irregular rods, exhibiting a marked rod-coccus growth cycle. They contained lysine-based peptidoglycan, teichulosonic acid and poly(glycosyl phosphate) polymers in the cell walls. The major menaquinone was MK-9(H₂), the predominant fatty acids were saturated, <i>anteiso</i>- and <i>iso</i>-branched, and the major compounds of polar lipid profiles included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two glycolipids (monogalactosyldiacylglycerol and dimannosylglyceride). The strains showed the highest 16S rRNA gene sequence similarity to <i>Arthrobacter crystallopoietes</i> (99.6–99.9%), followed by <i>A. mangrovi</i> (98.3–98.5%) and <i>A. globiformis,</i> the type species of the genus <i>Arthrobacter</i> (97.6–97.8%). The values of overall genome related indices (ANI, dDDH, AAI and POCP) indicated that the target strains and <i>A. crystallopoietes</i> represented three species within a new genus. These species clearly differed from each other and from <i>A. mangrovi</i> and <i>A. globiformis</i> in the phenotypic traits, including the peptidoglycan type and the structures and compositions of cell wall glycopolymers. Based on the findings of this study and the previously published data, we provide the description of the new genus <i>Crystallibacter</i> gen. nov. with the type species <i>Crystallibacter crystallifaciens</i> comb. nov. (type strain, VKM Ac-1107^T) and two newly revealed species, <i>Crystallibacter permensis</i> sp. nov. (type strain, B905^T = VKM Ac-2550^T = LMG 33113^T) and <i>Crystallibacter degradans</i> sp. nov. (type strain, SF27^T = VKM Ac-2063^T = LMG 33112^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142880526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description and genomic characterization of Mangrovibrevibacter kandeliae gen. nov. sp. nov., a novel carotenoid-producing endophytic bacterium isolated from branch of mangrove plants","authors":"Chengshao Zan,&nbsp;Zhouqing Zheng,&nbsp;Xiaohui Chen,&nbsp;Mingsheng Chen,&nbsp;Feina Li,&nbsp;Li Tuo","doi":"10.1007/s10482-024-02049-6","DOIUrl":"10.1007/s10482-024-02049-6","url":null,"abstract":"<div><p>Two Gram-stain-negative, aerobic, motile and short-rod-shaped bacterial strains, designated CSK15Z-1^T and MSK8Z-1, were isolated from branch of <i>Kandelia candel</i> and <i>Rhizophora stylosa</i>, respectively, collected from Shankou Mangrove Nature Reserve in Guangxi, China, and had the ability to produce carotenoid. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) values, amino acid identity (AAI) values, and the digital DNA-DNA hybridization (dDDH) values between the two strains indicated that they should be affiliated with the same species. The AAI values between the two strains and other related species in the family <i>Aurantimonadaceae</i> ranged between 61.0 and 66.6%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the two strains belong to the family <i>Aurantimonadaceae</i> and were most closely related to <i>Aurantimonas endophytica</i> EGI 6500337^T and <i>Aureimonas glaciistagni</i> PAMC 27157^T. Phylogenomic analysis based on genome sequences showed that the two strains formed a single and stable branch within the family <i>Aurantimonadaceae</i>. The major respiratory quinone was Q-10. The predominant fatty acids were C_18:1<i>ω</i>7c, C_19:0 cycle <i>ω</i>8c and C_16:0. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminolipid and an unidentified lipid. In accordance with the AAI values, phylogenomic and phylogenetic analysis, phenotypic and chemotaxonomic characteristics, and genomic characterization, strains CSK15Z-1^T and MSK8Z-1 should be considered as a novel genus and species in the family <i>Aurantimonadaceae</i>, for which the name <i>Mangrovibrevibacter kandeliae</i> gen. nov. sp. nov. is proposed. The type strain is CSK15Z-1^T (= MCCC 1K07183^T = JCM 34923^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerasicoccus fimbriatus sp. nov., isolated from the mid-ridge of the Southwest Indian Ocean","authors":"Xuanyun Qiu,&nbsp;Beihan Chen,&nbsp;Dan Lin,&nbsp;Guohua Liu,&nbsp;Zhiyi Su,&nbsp;Mingzhe Zhang,&nbsp;Kai Tang","doi":"10.1007/s10482-024-02047-8","DOIUrl":"10.1007/s10482-024-02047-8","url":null,"abstract":"<div><p>A Gram-stain-negative bacterium, designated as strain TK19100^T, was isolated from the mid-ridge of the Southwest Indian Ocean. Cells of strain TK19100^T were strictly aerobic, non-motile and short-rod shaped with fimbriae-like structures around the cell surface. Growth occurred at 15–40 °C, at pH 6.0–9.0 and with 1–10% (w/v) NaCl. Strain TK19100^T shared the highest 16S rRNA gene sequence similarity with <i>Cerasicoccus frondis</i> NBRC 105381^T of 97.74%, followed by <i>Cerasicoccus arenae</i> KCTC 12870^T of 97.69% and <i>Cerasicoccus maritimus</i> NBRC 105382^T of 97.40%. The phylogenetic tree based on both 16S rRNA genes and 92 core genes placed strain TK19100^T in a new linage within the genus <i>Cerasicoccus</i>. The genome size of strain TK19100^T was 5.07 Mb with the DNA G + C content 56.1%. The average nucleotide identity value and the digital DNA-DNA hybridization value of strain TK19100^T compared with the closest related species, <i>Cerasicoccus frondis</i>, were 78.39% and 23.70%, respectively. Strain TK19100^T encoded a previously unreported combination of GH16 and Carbohydrate Binding Module 96. The major fatty acids of strain TK19100^T were C_14:0 and C_18:1 <i>ω</i>9<i>c</i>. Menaquinone-7 was the sole respiratory quinone. The phenotypic and genotypic characterization analysis indicate that strain TK19100^T represents a novel species affiliated to the genus <i>Cerasicoccus</i>, for which the name <i>Cerasicoccus fimbriatus</i> sp. nov. is proposed. The type strain is TK19100^T (= CGMCC 1.18957^T = NBRC 116189^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jannaschia ovalis sp. nov., a marine bacterium isolated from a tidal flat sediment in the Republic of Korea","authors":"Dae Young Kwon,&nbsp;Sang Hyun Lee,&nbsp;Jong Seong Khim,&nbsp;Xiaoyue Xu,&nbsp;Bogun Kim,&nbsp;In-Geol Choi,&nbsp;Yeonjae Yoo,&nbsp;Jae-Jin Kim","doi":"10.1007/s10482-024-02044-x","DOIUrl":"10.1007/s10482-024-02044-x","url":null,"abstract":"<div><p>A Gram-stain-negative, non-motile, aerobic, pale-yellow coloured and oval-shaped bacterium designated GRR-S6-38^T was isolated from a tidal flat sediment that collected from Garorim Bay of the Yellow Sea, Republic of Korea. Strain GRR-S6-38^T grew at 15–40 °C (optimum, 30 °C) at pH 6–9 (optimum, pH 7) and at 2–6% (w/v) NaCl (optimum, 2% NaCl). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GRR-S6-38^T was closely related to those of <i>J. seosinensis</i> CL-SP26^T (98.1%), <i>J. helgolandensis</i> DSM 14858^T (97.4%), <i>J. rubra</i> CECT 5088^T (97.4%), <i>J. faecimaris</i> DSM 100420^T (97.4%), <i>J. marina</i> SHC163<i>T</i> (97.3%), <i>J. donghaensis</i> CECT 7802^T (97.2%) and <i>J. pohangensis</i> DSM 19073^T (97.1%). The average nucleotide identity and digital DNA-DNA hybridization value between GRR-S6-38^T and related type strains were 71.47–78.59% and 17.80–21.40%. Strain GRR-S6-38^T was characterized as having Q-10 as the predominant respiratory quinone and the major principle fatty acids (&gt; 10%) were SF 8 (C_18:1 <i>ω</i>7<i>c</i>/C_18:1 <i>ω</i>6<i>c</i>, 39.3%), C_19:0 <i>ω</i>8<i>c</i> cyclo (13.2%) and C_18:1 <i>ω</i>7<i>c</i> 11-methyl (10.6%). The polar lipids consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified lipid, two unidentified aminolipids and two unidentified phospholipids. The assembled genome of strain GRR-S6-38^T has a total length of 3.32 Mbp, and a G + C content is 69.98%. Based on the polyphasic taxonomic evidence presented in this study, GRR-S6-38^T is regarded to represent a novel species within the genus <i>Jannaschia</i>, for which name <i>Jannaschia ovalis</i> sp. nov. is proposed. The type strain is GRR-S6-38^T (= KCTC 82518 = KACC 22240 = JCM 36187).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A description of Joostella sp. strain CR20 with potential biotechnological applications","authors":"Muhammad Ramziuddin Zakaria,&nbsp;Maša Vodovnik,&nbsp;Maša Zorec,&nbsp;Kok Jun Liew,&nbsp;Lili Tokiman,&nbsp;Chun Shiong Chong","doi":"10.1007/s10482-024-02045-w","DOIUrl":"10.1007/s10482-024-02045-w","url":null,"abstract":"<div><p>The underexplored halophilic genus <i>Joostella</i> within the <i>Flavobacteriaceae</i> family consists of only two species, both of which have received little attention for their potential biotechnological applications. In this study, we report the isolation and characterisation of a novel halophilic bacterium, strain CR20, using a genomic approach to investigate its biotechnological potential. Analysis of the 16S rRNA gene revealed that strain CR20 shares 97.5% and 96.2% sequence similarity with <i>Joostella marina</i> DSM 19592^T and <i>Joostella atrarenae</i> M1-2^T, respectively. Strain CR20 exhibited average nucleotide identity and digital DNA-DNA hybridisation values of 76.8–79.1% and 20.8–22.8%, respectively, with <i>Joostella</i> spp., which fall below the species delineation thresholds. Additionally, strain CR20 demonstrated average amino acid identity and percentage of conserved proteins values of 81.3–84.0% and 71.7–75.3%, respectively, with <i>Joostella</i> spp., above the genus delineation thresholds. Meanwhile, the average amino acid identity and percentage of conserved proteins values of strain CR20 against <i>Galbibacter</i> spp. are 73.9–80.0% and 61.3–72.3%, respectively, also above the genus delineation thresholds. These findings indicated strain CR20 has a close relationship with both genera. Chemotaxonomic analysis of strain CR20 identified predominant fatty acids, including iso-C_17:0 3OH (25.3%), iso-C_15:0 (14%), and C_16:1 <i>ω</i>6<i>c</i>/C_16:1 <i>ω</i>7<i>c</i> (12.2%). The assembled genome comprises 62 contigs, with a size of approximately 3,168,727 bp and a G + C content of 35.1%. Among 2,804 predicted genes, 2,559 were classified into 25 COG functional groups. A total of 68 genes with potential industrial applications were identified, including 1 β-mannanase, 2 β-xylosidases, 1 polysaccharide deacetylase, 4 other hemicellulases, 6 β-glucosidases, 25 proteases, and 29 phosphate-solubilising enzymes. Hydrolytic assays confirmed that strain CR20 produces these enzymes extracellularly. These findings highlight strain CR20 has potential for industrial applications.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}