Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Isoalcanivorax beigongshangi sp. nov., isolated from the fermented grains of Chinese baijiu","authors":"Tong-Xi Zhang,&nbsp;Yi-Ming Li,&nbsp;Hao-Yue Gu,&nbsp;Ru Zhang,&nbsp;Zhan-Bin Sun,&nbsp;Han-Xu Pan,&nbsp;Qing Ren","doi":"10.1007/s10482-024-02043-y","DOIUrl":"10.1007/s10482-024-02043-y","url":null,"abstract":"<div><p>A bacterial strain, REN37^T, was isolated from fermented grains of Baijiu samples collected from Sichuan, PR China. The cells of strain REN37^T was Gram-negative and aerobic. The cellular morphology exhibited rod-shaped cells without flagellum, displaying non-motility. The optimal growth condition was at 32–37 °C, pH 6.0–7.0, and with a NaCl concentration of 1–2% (w/v). Based on the analysis of 16S rRNA gene sequence, strain REN37^T was identified to belong to the genus of <i>Isoalcanivorax.</i> Its closest species was <i>Isoalcanivorax pacificus</i> W11-5^T (96.1%). The polar lipids were identified to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, unidentified glycolipid, two unidentified phospholipids. The predominant menaquinone was MK-12. The predominant fatty acids were C_15:0 anteiso (49.4%), C_16:0 iso (18.1%), C_17:0 anteiso (16.5%) and C_15:0 iso (9.5%). The digital DNA-DNA hybridization (dDDH), average nucleotide identity (OrthoANI) and average amino acid identify (AAI) values between strain REN37^T and its most similar species were 19.2%, 74.0% and 78.0%, respectively. The DNA G + C content of the strain REN37^T was 63.0 mol%. Three alkane 1-monooxygenase (<i>alkB</i>1) genes (1152 bp, 1149 bp, 1179 bp) were identified in the genome, indicating that strain REN37^T may be associated with efficient oil-degradation capabilities. Based on the results, REN37^T represents a novel specie, and the name <i>Isoalcanivorax beigongshangi</i> sp<i>.</i> nov. was proposed. The type strain is REN37^T (= GDMCC 1.3120^T = JCM 35319^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facivitalis istanbulensis gen. nov., sp. nov., a novel member of the family Sphingomonadaceae with the potential for aromatic-degradation isolated from Jet A1 fuel","authors":"Simge Arkan-Ozdemir,&nbsp;Miray Üstüntürk-Onan,&nbsp;Esra Ilhan-Sungur","doi":"10.1007/s10482-024-02037-w","DOIUrl":"10.1007/s10482-024-02037-w","url":null,"abstract":"<div><p>A novel gram-stain-indeterminate, rod-shaped, endospore-forming, motile, aerobic bacterium, designated JETA1-E2^T, was isolated from aircraft fuel Jet A1 sample. The strain showed high pairwise similarity values of partial 16S rRNA gene sequences to <i>Sphingomonas paucimobilis</i> (MT367853) (99.42%), <i>Sphingomonas sanguinis</i> (MF319771) (99.34%), and <i>Sphingomonas pseudosanguinis</i> (HE716953) (99.27%) within the family <i>Sphingomonadaceae</i>. However, API test results revealed that the strain JETA1-E2^T differed from these type strains. The phylogenetic tree based on the whole genome and the phylogenomic tree generated with the UBCG tool showed that the strain JETA1-E2^T formed a distinct monophyletic clade within the family <i>Sphingomonadaceae</i>, and clustered distantly with the genera <i>Sphingomonas</i> and <i>Sphingobium</i>. The predominant respiratory quinone is Q-10. The major fatty acids are C_16:0 and summed feature 8 (C_18:1<i>ω</i>7<i>c</i> and/or C_18:1<i>ω</i>6<i>c</i>). C_19:0 is present in small amounts. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, aminophospholipid, unidentified glycolipid, and two unidentified sphingoglycolipids. The only polyamine is putrescine in minor amounts. The DNA G + C content of the type strain is 66.5 mol%. Several unique genes in the strain JETA1-E2^T may contribute to fight against various stressors, virulence and pathogenicity, as well as survival in challenging conditions. The strain JETA1-E2^T contains 100 of the characterised proteins available in the HADEG database of which 58% of these are involved in metabolic process of aromatics degradation. The findings indicate that the strain JETA1-E2^T has the potential to metabolise hydrocarbons such as fuel, especially aromatic compounds. Based on the results of polyphasic taxonomic analyses, the strain JETA1-E2^T represents a novel species in a new genus in the family <i>Sphingomonadaceae</i> for which the name <i>Facivitalis istanbulensis</i> gen. nov., sp. nov. is proposed. The type strain of <i>Facivitalis istanbulensis</i> is JETA1-E2^T (DSM 117971^T = LMG 33634^T = KUEN 1206 (B) F3-1-1^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic analyses of aerobic planctomycetes isolated from the deep sea and the ocean surface","authors":"Lise Øvreås,&nbsp;Nicolai Kallscheuer,&nbsp;Rita Calisto,&nbsp;Nicola Bordin,&nbsp;Julia E. Storesund,&nbsp;Christian Jogler,&nbsp;Damien Devos,&nbsp;Olga Lage","doi":"10.1007/s10482-024-02041-0","DOIUrl":"10.1007/s10482-024-02041-0","url":null,"abstract":"<div><p>On the deep and dark seafloor, a cryptic and yet untapped microbial diversity flourishes around hydrothermal vent systems. This remote environment of difficult accessibility exhibits extreme conditions, including high pressure, steep temperature- and redox gradients, limited availability of oxygen and complete darkness. In this study, we analysed the genomes of three aerobic strains belonging to the phylum <i>Planctomycetota</i> that were isolated from two deep-sea iron- rich hydroxide deposits with low temperature diffusive vents. The vents are located in the Arctic and Pacific Ocean at a depth of 600 and 1,734 m below sea level, respectively. The isolated strains Pr1d^T, K2D and TBK1r were analyzed with a focus on genome-encoded features that allow phenotypical adaptations to the low temperature iron-rich deep-sea environment. The comparison with genomes of closely related surface-inhabiting counterparts indicates that the deep-sea isolates do not differ significantly from members of the phylum <i>Planctomycetota</i> inhabiting other habitats, such as macroalgae biofilms and the ocean surface waters. Despite inhabiting extreme environments, our “deep and dark”-strains revealed a mostly non-extreme genome biology.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-024-02041-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nocardioides xinjiangensis sp. nov., a novel species isolated from desert soil","authors":"Yi-Jun Mo,&nbsp;Jun Liu,&nbsp;Jie Huang,&nbsp;Zhuo-Huan Zheng,&nbsp;Shuai Li,&nbsp;Lei Dong,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-024-02046-9","DOIUrl":"10.1007/s10482-024-02046-9","url":null,"abstract":"<div><p>Two novel strains, SYSU D00514^T and SYSU D00778, were isolated from desert soil in the Gurbantunggut Desert, Xinjiang Uygur Autonomous Regions, PR China. SYSU D00514^T and SYSU D00778 were aerobic, Gram-stain-positive, rod-shaped, catalase-positive and oxidase-negative. SYSU D00514^T grew at temperatures ranging from 4 to 37 °C (optimum, 28–30 °C), at pH 6.0–8.0 (optimum, 7.0), and tolerated NaCl concentrations from 0 to 7.5% (optimum, 0.5% w/v). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains SYSU D00514^T and SYSU D00778 were closely related to the members of the genus <i>Nocardioides</i>. Two isolates were most closely related to <i>Nocardioides alpinus</i> Cr7-14^T (98.05% and 98.19%). The G + C content of the genomic DNA of strains SYSU D00514^T and SYSU D00778 were both 72.8%. Strain SYSU D00514^T and the type strains of the genus <i>Nocardioides</i> in the analysis had average nucleotide identity values of 76.8–85.8% as well as digital DNA–DNA hybridization values between 19.5% and 28.0%. The predominant cellular fatty acids of strain SYSU D00514^T were C_17:1 <i>ω</i>8<i>c</i>, C_18:1 <i>ω</i>9<i>c</i> and iso-C_16:0. For strain SYSU D00514^T, the major polar lipids encompassed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified aminolipids. The main respiratory quinone of strain SYSU D00514^T was MK-8 (H4). Based on the results of polyphasic taxonomic experiments, we propose that strains SYSU D00514^T and SYSU D00778 represent a novel species of the genus <i>Nocardioides</i>, with the name <i>Nocardioides xinjiangensis</i>. The type strain is SYSU D00514^T (= CGMCC 1.18622^T = MCCC 1K05001^T = KCTC 49488^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halobacillus rhizosphaerae sp. nov., a moderately halophilic bacterium with protease activities isolated from the rhizosphere soil of the mangrove Acanthus ebracteatus","authors":"Doudou Yin,&nbsp;Junjie Xie,&nbsp;Rui Liu,&nbsp;Chaodong Song,&nbsp;Ying Liang,&nbsp;Hongzhi Huang,&nbsp;Yihui Huang,&nbsp;Ruiting Long,&nbsp;Naikun Shen,&nbsp;Bing Yan,&nbsp;Hongyan Zhang","doi":"10.1007/s10482-024-02040-1","DOIUrl":"10.1007/s10482-024-02040-1","url":null,"abstract":"<div><p>A Gram-positive, moderately halophilic, rod-shaped and facultatively anaerobic strain T66^T, was isolated from the rhizosphere soil of mangrove <i>Acanthus ebracteatus</i> in Guangxi, China. Strain T66^T was observed to grow at 15–40 ℃ (optimum 30 ℃), pH 5–8 (optimum 6–7) and with 2–20% (w/v) NaCl (optimum 6%). The phylogenetic tree of the 16S rRNA gene sequences depicted a cluster of strain T66^T to be closely related to <i>Halobacillus litoralis</i> SL-4^T (98.72%), <i>Halobacillus dabanensis</i> D-8^T (98.70%) and <i>Halobacillus campisalis</i> ASL-17^T (98.66%). The phylogenetic analysis based on the 16S rRNA gene sequence showed that strain T66^T belonged to the genus <i>Halobacillus</i> and formed a separate branch. The digital DNA-DNA hybridisation and average nucleotide identify values between strain T66^T and its related species were 17.80–19.80% and 70.78–73.22%, respectively, which were lower than the threshold recommended for species delineation. The genomic DNA G + C content was 40.9%. The predominant isoprenoid quinone was MK-7, the cell-wall peptidoglycan contained meso-diaminopimelic acid, and the major fatty acids that accounted for more than 10.0% were anteiso-C_15:0 (68.4%) and anteiso-C_17:0 (13.4%). Phosphatidylglycerol, diphosphatidylglycerol, three unidentified phospholipids and an unidentified glycolipid were found in the polar lipid extraction. Strain T66^T could hydrolyse casein and contains several genes (e. g. <i>vanY, vpr, pbpG</i> and <i>elpC</i>) involved in protease. Therefore, strain T66^T is salt-tolerant and produces protease, and it may have development and utilisation value in flavouring agents and food curing. Based on phenotypic, chemotaxonomic data and genotypic characteristics, strain T66^T represents a novel species of the genus <i>Halobacillus</i>, for which the name <i>Halobacillus rhizosphaerae</i> sp. nov. is proposed. The type strain is T66^T (= JCM 36534^T = MCCC 1K08701^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the Mycobacteroides abscessus pan-genome using computational tools: insights into evolutionary dynamics and lifestyle","authors":"Mistu Karmakar,&nbsp;Saubashya Sur","doi":"10.1007/s10482-024-02042-z","DOIUrl":"10.1007/s10482-024-02042-z","url":null,"abstract":"<div><p><i>Mycobacteroides abscessus</i> is a non-tuberculous mycobacteria implicated in causing lung infections. It is difficult to control owing to resistance to antibiotics and disinfectants. This work was aimed at comprehending: the pan-genome architecture, evolutionary dynamics, and functionalities of pan-genome components linked to COGs and KEGG. Around 2802 core genes were present in each strain of the <i>M. abscessus</i> genome. The number of accessory genes ranged from 1615 to 2481. The open pan-genome of <i>M. abscessus</i> was attributed to the accessory genes underlining its adaptability in the host. Phylogenetic analysis revealed cluster-based relationships and highlighted factors shaping variability and adaptive capabilities. Transcription, metabolism, and pathogenic genes were vital for <i>M. abscessus</i> lifestyle<i>.</i> The accessory genes contributed to the diverse metabolic capability. The incidence of a significant portion of secondary metabolite biosynthesis genes provided insights for investigating their biosynthetic gene clusters. Additionally, a high proportion of xenobiotic biodegradation genes highlighted potential metabolic capabilities. In silico screening identified a potential vaccine candidate among hypothetical proteins in COGs. Functional analysis of <i>M. abscessus</i> pan-genome components unveiled factors associated with virulence, pathogenicity, infection establishment, persistence, and resistance. Notable amongst them were: MMPL family transporters, PE-PPE domain-containing proteins, TetR family transcriptional regulators, ABC transporters, Type—I, II, III, VII secretion proteins, DUF domain-containing proteins, cytochrome P450, VapC family toxin, virulence factor Mce family protein, type II toxin-antitoxin system. Overall, these results enhanced understanding of the metabolism, host–pathogen dynamics, pathogenic lifestyle, and adaptations. This will facilitate further investigations for combating infections and designing suitable therapies.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142691871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylocystis borbori sp.nov., a novel methanotrophic bacterium from the sludge of a freshwater lake and its metabolic properties","authors":"Elena N. Kaparullina,&nbsp;Nadezhda V. Agafonova,&nbsp;Natalia E. Suzina,&nbsp;Denis S. Grouzdev,&nbsp;Nina V. Doronina","doi":"10.1007/s10482-024-02039-8","DOIUrl":"10.1007/s10482-024-02039-8","url":null,"abstract":"<div><p>A novel methanotrophic strain 9N^T was isolated from the sludge of a freshwater lake. Cells were aerobic, Gram-stain-negative, non-motile pleomorphic rods with intracytoplasmic membrane systems that appropriate type-II methanotrophs and hemispherical and spherical exocellular formations on the perimeter of the cell wall surface. The novel isolate grows only on methane or methanol as the sole carbon and energy source, at 10–37 °C (optimum 28–30 °C), pH 4.5–9.0 (optimum 7.0–7.5), up to 1% NaCl (optimum 0.3–0.5%). Methanol supported the growth of the strain 9N^T in a wide range of concentrations from 0.05 to 5.0% (v/v) with an optimum of 0.5% (v/v). The major fatty acids were C_18:1<i>ω</i>8<i>c</i> and C_18:1<i>ω</i>7<i>c</i>. Based on 16S rRNA gene sequence phylogenetic analysis, strain 9N^T was closely related to representatives of the genus <i>Methylocystis</i> (96.5–98.3%). The genome of strain 9N^T was 3.34 Mbp in size with 63.5% of G + C content. The average nucleotide identity and digital DNA–DNA hybridization values between strain 9N^T and closely related type strains of genus <i>Methylocystis</i> were 78.0–82.4% and 20.9–24.3%, respectively. The careful genome annotation of the novel strain shows it possessed genes for the detoxification of arsenate and cyanides as well as genes potentially involved in plant growth promotion (such as biosynthesis of indoles, cytokinins, polyhydroxybutyrate, siderophore production, and nitrogen fixation). Based on the phylogenetic, phenotypic, chemotaxonomic and genomic data, we propose <i>Methylocystis borbori</i> sp. nov. as novel species of the genus <i>Methylocystis</i>. The type strain is 9N^T (= VKM B-3616^T = KCTC 92566^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142679642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclassification of two Nocardiopsis species using whole genome analysis","authors":"Guendouz Dif,&nbsp;Nadjette Djemouai,&nbsp;Noureddine Bouras,&nbsp;Abdelghani Zitouni","doi":"10.1007/s10482-024-02038-9","DOIUrl":"10.1007/s10482-024-02038-9","url":null,"abstract":"<div><p>This study revisits the taxonomic classification of the genus <i>Nocardiopsis</i> through the application of advanced genome-based methodologies, representing a notable advancement beyond traditional approaches that primarily rely on 16S rRNA gene sequences and phenotypic traits. The advent of Next-Generation Sequencing (NGS) and sophisticated bioinformatic tools has enabled a more precise framework for prokaryotic classification. However, many recognized species still lack complete genome sequences. In this study, we employed overall genome-related indices (OGRI), particularly Average Nucleotide Identity (ANI) and digital DNA-DNA hybridization (dDDH), to reassess the phylogenetic relationships within the genus. Comparative analyses of complete 16S rRNA sequences demonstrated high genetic similarity between <i>N</i>. <i>umidischolae</i> 66/93^T and <i>N</i>. <i>tropica</i> VKMAc-1457^T (99.61% similarity), as well as between <i>N</i>. <i>rhodophaea</i> JCM15313^T and <i>N</i>. <i>rosea</i> JCM15314^T (100% identity). The dDDH, ANI%, FastANI%, ANIm, and ANIb values further supported these results, with 73.2%, 96.94%, 96.15%, 97.19%, and 96.74% for the former pair, and 93.6%, 99.13%, 99.97%, 99.98%, and 99.27% for the latter pair, respectively. All values surpassed the species delineation thresholds of 70% for dDDH and 95–96% for ANI. Consequently, we propose reclassifying <i>N</i>. <i>umidischolae</i> as a heterotypic synonym of <i>N</i>. <i>tropica</i>, and <i>N</i>. <i>rosea</i> as a heterotypic synonym of <i>N</i>. <i>rhodophaea</i>, in accordance with the International Code of Nomenclature of Prokaryotes (ICNP).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142672518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Isoptericola haloaureus sp. nov., a dimorphic actinobacterium isolated from mangrove sediments of southeast India, implicating biosaline agricultural significance through nitrogen fixation and salt tolerance genes","authors":"Munisamy Prathaban,&nbsp;Ragothaman Prathiviraj,&nbsp;Mythili Ravichandran,&nbsp;Sharmila Devi Natarajan,&nbsp;Murugesan Sobanaa,&nbsp;S. Hari Krishna Kumar,&nbsp;Varadharaju Chandrasekar,&nbsp;Joseph Selvin","doi":"10.1007/s10482-024-02033-0","DOIUrl":"10.1007/s10482-024-02033-0","url":null,"abstract":"","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic approach to determine the outcome of the competition between two microbial species in bioreactor cocultures","authors":"Marcin Bizukojć,&nbsp;Tomasz Boruta,&nbsp;Anna Ścigaczewska","doi":"10.1007/s10482-024-02035-y","DOIUrl":"10.1007/s10482-024-02035-y","url":null,"abstract":"<div><p>The two-species microbial cocultures are effective in terms of awakening the cryptic biosynthetic pathways. They may also lead to the improved production of previously discovered molecules. Importantly, only a few outcomes of the cocultures may prove desirable, namely those leading to the formation of useful secondary metabolites. To address this issue, a method allowing for the evaluation of the final outcome of the co-culture process and fine-tune the cocultivation strategy was proposed. The systematic approach was supported by the experimental data from the bioreactor runs with the participation of <i>Aspergillus terreus</i> and <i>Penicillium rubens</i> confronted with <i>Streptomyces rimosus</i> and <i>Streptomyces noursei</i>. Kinetic, morphological and metabolic aspects of dominance were analysed via the newly proposed formula describing the dominance pattern. The suggested method involved the determination of the numerical value representing the dominance level. When it was high (value 1) no useful metabolites were formed apart from those originating from the winning counterpart. But either for the partial dominances or when the winning organism changed within the run or when the competition ended in draw, the number of the secondary metabolites of interest in the broth was the highest. Next, the systematic approach illustrated how the delayed inoculation strategy influenced the level of dominance leading to the change of winning counterpart and the set of metabolites produced. The proposed systematic approach allows for the reliable determination of the level of dominance in the two-species cocultures to seek for the potentially useful substances for future applications.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-024-02035-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142632166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}