Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Methanosarcina acetivorans requires methanol:coenzyme M methyltransferases for ethane formation from ethanol","authors":"Tejas Somvanshi,&nbsp;Mai Anh Tran,&nbsp;Jichen Bao,&nbsp;Silvan Scheller","doi":"10.1007/s10482-025-02165-x","DOIUrl":"10.1007/s10482-025-02165-x","url":null,"abstract":"<div><p>Corrinoid-dependent enzymes either catalyze methyltransfer reactions, or they generate substrate radicals using adenosylcobalamin for subsequent rearrangement reactions. The corrinoid-dependent methyltransferases are present in all domains of life and assumed to be exclusive for methyl-groups. In <i>Methanosarcina</i>, however, trace ethane production from ethanol has been shown in vivo<i>,</i> which led to the hypothesis that corrinoid-dependent methanol-specific methyltransferases are promiscuous towards also accepting ethyl-groups. Here, we show that the conversion of ethanol to trace amounts of ethane in <i>Methanosarcina acetivorans</i> involves homologous reactions of the known methanol-to-methane metabolism. The methanol methyltransferase (MtaB) activates ethanol and loads the ethyl-group onto the corrinoid-containing methyl-accepting protein (MtaC). Besides MtaCB, substrate promiscuity in corrinoid:coenzyme M methyltransferase (MtaA) and methyl-coenzyme M reductase (Mcr) are required to grant the microbe the capacity for ethane production. We show that the MtaCB subunits of <i>M. acetivorans</i> can activate ethanol, however, the ethane yields compared to methane are ca. 3 orders of magnitude lower. The ethyl-transfer capability was confirmed for each of the three MtaCB isozyme by quantifying the amount of ethane produced by <i>mtaCB</i> double deletion strains during growth in ethanol-supplemented media and in resting-cell suspensions. Ethane formation requires the cells to be grown on methanol to trigger the expression of the <i>mtaCB</i> genes, and detectable ethane formation starts only after all methanol has been consumed. Demonstrating that corrinoid-dependent methanol-specific methyltransferases process ethyl groups extends the pool of reactions to be considered in metabolic networks and suggests possible routes for biogenic ethane in nature.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-025-02165-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of prodigiosin on Bacillus cereus-induced intestinal infection in mice","authors":"Jinlong Jiao,&nbsp;Dandan Zeng,&nbsp;Miao Zou,&nbsp;Tianlu Mo","doi":"10.1007/s10482-025-02167-9","DOIUrl":"10.1007/s10482-025-02167-9","url":null,"abstract":"<div><p><i>Bacillus cereus</i> is a significant foodborne pathogen whose infection can trigger gastrointestinal symptoms or systemic diseases in hosts, with severe cases potentially leading to sepsis or even death. In recent years, the misuse of antibiotics has led to varying degrees of drug resistance in foodborne pathogens, making the development of novel antimicrobial agents an urgent necessity. Natural active substances have become a focal point of current research due to their broad-spectrum and highly efficient antibacterial capabilities against foodborne pathogens. Prodigiosin (PG), initially garnering widespread attention in the pharmaceutical field for its multifunctional bioactivities, also demonstrates significant potential in the food industry. However, there is currently no reported research on the antibacterial effects of PG against <i>B. cereus</i>, its anti-infection mechanisms, and its impact on the host's intestinal microbiota. Based on this, our study focuses on PG as the research subject and <i>B. cereus</i> as the test strain, systematically investigating its therapeutic effect in <i>B. cereus</i>-infected mice. Additionally, we employed 16S rRNA-based microbiota analysis and metabolomics approaches to explore the effects of PG on the composition of the gut microbiota and metabolomic profiles. Collectively, PG exerts its therapeutic effects in <i>B. cereus</i>-infected mice by alleviating tissue damage, reducing inflammation, and modulating the abundance and diversity of the intestinal microbiota. These results highlight a novel strategy for alleviating <i>B. cereus</i> infections<i>.</i></p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on microbial-biofilm mediated mechanisms in marine microplastics degradation","authors":"Soumyajit Chandra,&nbsp;Dibyajit Lahiri,&nbsp;Moupriya Nag,&nbsp;Debasmita Bhattacharya,&nbsp;Chetan Pandit,&nbsp;Soumya Pandit,&nbsp;Kuldeep Sharma,&nbsp;Mithul Rajeev,&nbsp;Harjot Singh Gill,&nbsp;Sarvesh Rustagi","doi":"10.1007/s10482-025-02163-z","DOIUrl":"10.1007/s10482-025-02163-z","url":null,"abstract":"<div><p>Although the buildup of plastic in the marine environment is a long-standing problem, it has only lately been realized how relevant this pollution may be to the ocean. Microplastic fragments are an emerging concern in this field. Due to their small size, they are easily consumed by marine animals and, combined with the poisons and bacteria that colonize the plastic, can accumulate in the food chain. Unlike non-plastic biofilms, plastic biofilms are diverse and driven by a complex web of elements, including seasonal and spatial parameters and substratum characteristics like size, texture, and polymer type. The major topic of the paper is the discussion of preliminary findings and knowledge gaps about microbial biofilm communities connected to microplastics. Additionally, the review addresses microplastic sources, associated toxicity, and the role of both bacterial and fungal communities in their degradation.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial modulators of the mind: probiotic interventions in hippocampal neurogenesis and cognitive flexibility","authors":"Jianghua Zhao,&nbsp;Huiquan Liu,&nbsp;Bita Badehnoosh","doi":"10.1007/s10482-025-02162-0","DOIUrl":"10.1007/s10482-025-02162-0","url":null,"abstract":"<div><p>The gut-brain axis serves as a foundational communication channel between the intestinal microbiome and the brain, facilitating microbial impact on neural functions. Probiotics, defined as health-promoting live microorganisms, are being increasingly investigated for their regulatory effects on neuroplasticity and mental acuity. Recent evidence suggests that probiotics modulate hippocampal neurogenesis, a crucial process underlying learning, memory, and cognitive flexibility. Through the suppression of pro-inflammatory mechanisms, enhancement of neurotrophic factor biosynthesis, alleviation of oxidative burden, and stabilization of HPA axis function, probiotics contribute to sustaining hippocampal neural resilience and promoting synaptic adaptability. Evidence from both preclinical experiments and clinical evaluations suggests that strains like <i>Lactobacillus rhamnosus</i>, <i>Bifidobacterium longum</i>, and <i>Lactobacillus plantarum</i> may play a beneficial role in promoting adaptive cognitive functioning. These benefits are thought to be mediated via increased expression of brain-derived neurotrophic factor, modulation of microglial activation, and alteration of neurotransmitter metabolism including serotonin, dopamine, and GABA. This review synthesizes current findings on the molecular and cellular pathways through which probiotics support hippocampal neurogenesis and cognitive flexibility, and discusses their potential as a non-invasive, adjuvant strategy for cognitive enhancement in neurological disorders and age-related cognitive decline.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Four novel species of the genus Aquimarina isolated from marine sponges: Aquimarina callyspongiae sp. nov., Aquimarina mycalae sp. nov., Aquimarina discodermiae sp. nov., and Aquimarina penaris sp. nov","authors":"Yea-Lin Moon,&nbsp;Seung-Min Han,&nbsp;Kyung Hyun Kim,&nbsp;Jin-Sook Park","doi":"10.1007/s10482-025-02156-y","DOIUrl":"10.1007/s10482-025-02156-y","url":null,"abstract":"<div><p>Four Gram-stain-negative, strictly aerobic, catalase- and oxidase-positive bacterial strains, designated 2201CG5-10^T, 2201CG14-23^T, 2201CG1-2-11^T, and 2304DJ70-9^T were isolated from marine sponges collected in the Republic of Korea. Phylogenetic analyses based on 16S rRNA gene and whole-genome sequences revealed that these strains represent a distinct phyletic lineage within the genus <i>Aquimarina</i>. Based on the whole-genome sequence comparisons, the closest phylogenetic relatives of the four novel strains were <i>Aquimarina latercula</i> DSM 2041^T, <i>Aquimarina pacifica</i> SW150^T, and <i>Aquimarina mytili</i> PSC33^T, which shared average nucleotide identity values below 81.3% and digital DNA–DNA hybridization values below 22.0% with the novel strains. The genomic DNA G + C contents of strains 2201CG5-10^T, 2201CG14-23^T, 2201CG1-2-11^T, and 2304DJ70-9^T were 32.5, 32.5, 33.5, and 34.0%, respectively. Menaquinone-6 was the sole isoprenoid quinone, and the predominant fatty acids were iso-C_15:0, iso-C_15:1 G, and iso-C_17:0 3-OH. Phosphatidylethanolamine was the major polar lipid. Based on a polyphasic taxonomic approach, strains 2201CG5-10^T (= KACC 23587^T = JCM 36754^T), 2201CG14-23^T (= KACC 23588^T = JCM 36755^T), 2201CG1-2-11^T (= KACC 23831^T = JCM 33063^T), and 2304DJ70-9^T (= KACC 23830^T = JCM 33063^T = MCCC 1K09582^T) represent four novel species of the genus <i>Aquimarina</i>, for which the names <i>Aquimarina callyspongiae</i> sp. nov., <i>Aquimarina mycalae</i> sp. nov., <i>Aquimarina discodermiae</i> sp. nov., and <i>Aquimarina penaris</i> sp. nov. are proposed.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and green synthesis of iron oxide nanoparticles from Ureibacillus chungkukjangi for methylene blue degradation","authors":"Muhammad Naveed,&nbsp;Muhammad Asim,&nbsp;Tariq Aziz,&nbsp;Shumaila Ibrahim,&nbsp;Maida Salah Ud Din,&nbsp;Maryam M. Alomran,&nbsp;Ashwag Shami,&nbsp;Fakhria A. Al-Joufi,&nbsp;Ahmad A. Alghamdi,&nbsp;Abdullah A. Alqasem,&nbsp;Ahmed M. Basri,&nbsp;Majid Alhomrani","doi":"10.1007/s10482-025-02157-x","DOIUrl":"10.1007/s10482-025-02157-x","url":null,"abstract":"<div><p>Synthetic dyes, such as methylene blue (MB), are increasingly becoming sources of water pollution and require better treatment strategies. This study describes an eco-friendly method for methylene blue degradation using green synthesized iron oxide nanoparticles form <i>Ureibacillus chungkukjangi</i>. This bacterium was isolated from clinical samples and identified using 16S rRNA gene amplification and sequenced using Sanger sequencing technology. The identified <i>Ureibacillus chungkukjangi</i> was submitted to NCBI with NCBI accession no. PQ568249.1. The secondary metabolites of the bacteria acted as capping agents to both reduce and stabilize the nanoparticle synthesis. The nanoparticle synthesis was achieved by the addition of iron chloride solution as a precursor to bacterial metabolites, forming the orange-brown solution to dark brown that showed initial signs of nanoparticle synthesis that were verified with UV–Vis Absorption spectra giving peaks at 380 nm. In FTIR spectra of the range examined (570–630 cm⁻¹), Fe–O bonds were observed, which confirms that biofunctionalization of the surface had been done. Also observed were O–H, C–H, C=O, and C–O functional groups of surfaces biofunctionalization. Furthermore, SEM analysis showed the particle size ranging from 50 to 400 nm while massively polygonal, where EDX analysis further confirmed the presence of iron in the sample. The degradation studies conducted over 15 days showed that there was a total of 89% methylene blue degradation at a nanoparticle-to-dye ratio of 1:1. In contrast, the ratio of 1:5 only yielded a 79% degradation. Furthermore, the Fe₃O₄ NPs were shown to have powerful antioxidant activity (scavenging up to 93.2%), as well as inflammatory activity (82.3% inhibition), anti-hemolytic activity (84.4% inhibition), which suggests low toxicity and biocompatibility. This confirms the effectiveness of biosynthesized Fe₃O₄ NPs for the treatment of dye-contaminated water, utilizing them as a cost-effective and multifunctional approach, thus advancing the field of nano bioremediation.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144934773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of plant growth promoting rhizobacteria inoculation on growth and physiological traits of ginger in field conditions","authors":"Dilfuza Jabborova,&nbsp;Dilbar Kadirova,&nbsp;Zafarjon Jabbarov,&nbsp;Janki N. Thakker,&nbsp;Khushbu Rathod,&nbsp;Muzafar Jabborov,&nbsp;Ayush Madan,&nbsp;Mohammad Shah Jahan,&nbsp;Mehdi Rahimi,&nbsp;Amjad M. Husaini","doi":"10.1007/s10482-025-02161-1","DOIUrl":"10.1007/s10482-025-02161-1","url":null,"abstract":"<div><p>Plant growth-promoting rhizobacteria (PGPR) are known to enhance the productivity, development, yield, and soil health of both medicinal and vegetable crops. The present investigation evaluated the influence of PGPR on the growth attributes and physiological parameters of ginger, alongside soil quality, under field conditions. Field trials were carried out over three consecutive years (2020 to 2022), with ginger being planted each March. The experiment included six treatments: five PGPR strains—<i>Bacillus subtilis</i> IGPEB 1, <i>Bacillus pumilus</i> IGPEB 2, <i>Bacillus altitudinis</i> IGPEB 8, <i>Pseudomonas koreensis</i> IGPEB 17, and <i>Bacillus endophyticus</i> IGPEB 33—applied individually, along with an uninoculated control. These treatments were arranged in a randomized block design with five replications. Among the strains, <i>B. endophyticus</i> IGPEB 33 notably improved ginger’s morphological traits, including shoot length, leaf number, leaf length, leaf width, and overall yield, compared to the control. This strain also significantly boosted the content of chlorophyll (a and b), total chlorophyll, and carotenoids in ginger leaves. Furthermore, <i>B. endophyticus</i> IGPEB 33 enhanced soil nitrogen content as well as catalase and urease enzymatic activity. Notably, urease activity was also increased with the inoculation of <i>B. subtilis</i> IGPEB 1, <i>B. pumilus</i> IGPEB 2, and <i>P. koreensis</i> IGPEB 17. A significant positive correlation was observed between phosphorus levels and physiological parameters such as relative water content, photosynthetic pigments, and soil enzymes. Overall, this study highlights the effectiveness of PGPR in improving ginger growth and productivity through favorable modulation of plant physiology and soil biochemical properties, offering a promising approach for sustainable crop management. The positive correlations observed between PGPR inoculation, physiological traits, and yield performance suggest that these bacterial strains actively contribute to nutrient uptake, water retention, and stress tolerance mechanisms in ginger. Furthermore, their use promotes a sustainable and environmentally friendly alternative to chemical fertilizers, aligning with global efforts to reduce agricultural dependency on synthetic inputs. Given their effectiveness and potential scalability, these PGPR strains are strong candidates for development into commercial biofertilizer formulations.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144926991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duganella hordei sp. nov., Duganella caerulea sp. nov., and Duganella rhizosphaerae sp. nov., isolated from barley rhizosphere","authors":"Katsumoto Kishiro,&nbsp;Nurettin Sahin,&nbsp;Daisuke Saisho,&nbsp;Naoki Yamaji,&nbsp;Jun Yamashita,&nbsp;Yuki Monden,&nbsp;Tomoyuki Nakagawa,&nbsp;Keiichi Mochida,&nbsp;Akio Tani","doi":"10.1007/s10482-025-02160-2","DOIUrl":"10.1007/s10482-025-02160-2","url":null,"abstract":"<div><p><i>Duganella</i> sp. strains R1^T, R57^T, and R64^T, isolated from barley roots in Japan, are Gram-stain-negative, motile, rod-shaped bacteria. <i>Duganella</i> species abundantly colonized barley roots. Strains R1^T, R57^T, and R64^T were capable of growth at 4 °C, suggesting adaptation to colonize winter barley roots. Strains R57^T and R64^T formed purple colonies, indicating violacein production, while strain R1^T did not. Based on 16S rRNA gene sequence similarities, strains R1^T, R57^T, and R64^T were most closely related to <i>D. violaceipulchra</i> HSC-15S17^T (99.10%), <i>D. vulcania</i> FT81W^T (99.45%), and <i>D. violaceipulchra</i> HSC-15S17^T (99.86%), respectively. Their genome sizes ranged from 7.05 to 7.38 Mbp, and their genomic G+C contents were 64.2–64.7%. The average nucleotide identity and digital DNA–DNA hybridization values between R1^T and <i>D. violaceipulchra</i> HSC-15S17^T, R57^T and <i>D. vulcania</i> FT81W^T, R64^T and <i>D. violaceipulchra</i> HSC-15S17^T were 86.0% and 33.2%, 95.7% and 67.9%, and 92.7% and 52.6%, respectively. Their fatty acids were predominantly composed of C16:0, C17:0 cyclo, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). Based on their distinct genetic and phenotypic characteristics, and supported by chemotaxonomic analyses, we propose that strains R1^T, R57^T, and R64^T represent novel species within the <i>Duganella</i> genus, for which the names <i>Duganella hordei</i> (type strain R1^T = NBRC 115982^T = DSM 115069^T), <i>Duganella caerulea</i> (type strain R57^T = NBRC 115983^T = DSM 115070^T), and <i>Duganella rhizosphaerae</i> (type strain R64^T = NBRC 115984^T = DSM 115071^T) are proposed.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-025-02160-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus thuringiensis G-5 efficiently suppresses Codonopsis pilosula postharvest diseases by generating antifungal volatile organic compounds","authors":"Rongxiu Mo,&nbsp;Lijun Feng,&nbsp;Fanjin Kong,&nbsp;Yijuan Jiang,&nbsp;Wenyue Zhang,&nbsp;Lijun Ling","doi":"10.1007/s10482-025-02158-w","DOIUrl":"10.1007/s10482-025-02158-w","url":null,"abstract":"<div><p>Inappropriate storage conditions make <i>Codonopsis pilosula</i> more susceptible to infection by harmful fungi because of moisture, which reduces the plant's therapeutic effectiveness. The volatile organic compounds (VOCs) of plant endophytes have shown great promise in recent years for managing postharvest plant diseases. This study investigates the inhibitory effects of VOCs released by <i>Bacillus thuringiensis</i> G-5 on two postharvest pathogenic fungi of <i>C. pilosula</i>: <i>Fusarium oxysporum</i> F-3 and <i>Penicillium oxalicum</i> F-5. The results revealed that the mycelial growth inhibition rates of these fungi induced by the VOCs from G-5 were 94.01% and 95.37%, respectively. Scanning electron microscopy observations and Propidium Iodide staining experiments further confirmed that these VOCs could markedly alter the morphology and structure of mycelia and spores and compromise the integrity of the cell membrane. Headspace gas chromatography-ion mobility spectrometry analysis identified 21 high-concentration volatile substances released by G-5, with 3-hepten-2-one identified as the primary antifungal component. The minimum inhibitory concentrations of 3-hepten-2-one against F-3 and F-5 were determined to be 40 μL/L and 20 μL/L, respectively. Further research indicated that 3-hepten-2-one disrupts the structural and functional integrity of the cell membrane. In vivo experiments demonstrated that 3-hepten-2-one exhibited superior efficacy in preventing and controlling <i>C. pilosula</i> infections compared to the VOCs produced by G-5. This study not only provides a promising new antifungal agent for managing postharvest diseases of <i>C. pilosula</i> but also enhances our understanding of the role of VOCs produced by G-5 in biological control.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oleaginous yeasts from Brazilian Cerrado for sustainable biodiesel production using crude glycerol","authors":"Adalgisa Martínez,&nbsp;Angie Alvarez,&nbsp;Erika Arbildi,&nbsp;Irene Rocca,&nbsp;Gabriela Garmedia,&nbsp;Flavia Lucila Tonani,&nbsp;Raphael Sanzio Pimenta,&nbsp;Silvana Vero","doi":"10.1007/s10482-025-02159-9","DOIUrl":"10.1007/s10482-025-02159-9","url":null,"abstract":"<div><p>The global oversupply of crude glycerol, a byproduct of biodiesel production, needs innovative strategies for its sustainable utilization. In this study we isolated and characterized oleaginous yeast strains from fruit surfaces in the Brazilian Cerrado biome, a biodiversity hotspot, to assess their potential for converting crude glycerol into microbial lipids suitable for biodiesel. From 150 fruits, 45 yeast strains were isolated, with six identified as oleaginous (intracellular lipids &gt; 20% dry biomass). Molecular identification via ITS and D1/D2 sequencing analysis revealed affiliations with <i>Rhodotorula toruloides</i> and <i>Pseudozyma</i> species (<i>P. hubeiensis</i>, <i>P. flocculosa</i>, <i>P. rugulosa</i>). Lipid profiling showed predominant fatty acids (palmitic, oleic) aligning with biodiesel standards. Biodiesel properties derived from yeast lipids, including cetane number (58–62), viscosity (3.8–4.2 mm²/s), and density (864–902 kg/m³), complied with ASTM D6751 and EN14214 specifications, except for slightly elevated density in one strain. Rhodamine B screening demonstrated higher specificity for oleaginous yeasts compared to Nile Red. Phylogenetic analysis confirmed evolutionary relationships among isolates and type strains. These findings highlight the Cerrado’s microbial diversity as a reservoir for robust oleaginous yeasts, offering a dual solution for crude glycerol valorization and sustainable biodiesel production. The study underscores the potential of <i>Pseudozyma</i> spp. and <i>R. toruloides</i> for integrated biorefineries, combining lipid production with biosurfactant and enzyme synthesis to enhance economic viability.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}