Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Thiobacillus sedimenti sp. nov., a chemolithoautotrophic sulphur-oxidizing bacterium isolated from freshwater sediment","authors":"Chenming Dai,&nbsp;Guangye Zhang,&nbsp;Weitie Lin,&nbsp;Jianfei Luo","doi":"10.1007/s10482-024-02026-z","DOIUrl":"10.1007/s10482-024-02026-z","url":null,"abstract":"<div><p>A sulphur-oxidizing bacterium, designated strain SCUT-2^T, was isolated from freshwater sediment collected from the Pearl River in Guangzhou, PR China. This strain was an obligate chemolithoautotroph, utilizing reduced sulphur compounds (elemental sulphur, thiosulphate, tetrathionate and sulphite) as the electron donor. Growth of strain SCUT-2^T was observed at 20–40 ℃ (optimum at 30 °C), pH 5.0–9.0 (optimum at 6.0), and NaCl concentration range of 0–9 g L⁻¹ (optimum at 1 g L⁻¹). The major cellular fatty acids were C_16:0 ω7c and cyclo-C_17:0. The DNA G + C content of the complete genome sequence was 66.8 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCUT-2^T formed a lineage within the genus <i>Thiobacillus</i>, showing gene sequence identity of 98.0% with its closest relative <i>Thiobacillus thioparus</i> THI 115. The genome of strain SCUT-2^T contains multiple genes encoding sulphur-oxidizing enzymes that catalyse the oxidation of reduced sulphur compounds, partial genes that are necessary for denitrification, and the genes encoding <i>cbb</i>_<i>3</i>-type cytochrome c oxidase, <i>aa</i>_<i>3</i>-type cytochrome c oxidase and <i>bd</i>-type quinol oxidase. Facultative anaerobic growth occurs when using nitrate as the electron acceptor and thiosulphate as the electron donor. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic analysis, strain SCUT-2^T (= GDMCC 1.4108^T = JCM 39443^T) is deemed to represent a novel <i>Thiobacillus</i> species, for which we propose the name <i>Thiobacillus sedimenti</i> sp. nov.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paralabilibaculum antarcticum gen. nov., sp. nov., an anaerobic marine bacterium of the family Marinifilaceae isolated from Antarctica sea ice","authors":"Yifan Zhuang,&nbsp;Yunxiao Zhang,&nbsp;Wei Dai,&nbsp;Yantao Liang,&nbsp;Xiaoyu Yang,&nbsp;Yaru Wang,&nbsp;Xiaochong Shi,&nbsp;Xiao-Hua Zhang","doi":"10.1007/s10482-024-02022-3","DOIUrl":"10.1007/s10482-024-02022-3","url":null,"abstract":"<div><p>A novel bacterial strain, designated DW002^T, was isolated from the sea ice of Cape Evans, McMurdo Sound, Antarctica. Cells of the strain were Gram-negative, obligate anaerobic, motile, non-flagellated, and short rod-shaped. The strain DW002^T grew at 4–32 ℃ (optimum at 22–28 ℃) and thrived best at pH 7.0, NaCl concentration of 2.5% (w/v). The predominant isoprenoid quinone of strain DW002^T was menaquinone-7 (MK-7). The major fatty acids (&gt; 10%) of DW002^T were iso-C_15:0, anteiso-C_15:0 and iso-C_17:1<i>ω</i>9<i>c</i>. The predominant polar lipids of strain DW002^T contained two phosphatidylethanolamines, one unidentified glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G + C content of the strain DW002^T was 34.8%. Strain DW002^T encoded 237 carbohydrate-active enzymes. The strain DW002^T had genes associated with dissimilatory nitrate reduction and assimilatory sulfate reduction metabolic pathways. Based on distinct physiological, chemotaxonomic, genome analysis and phylogenetic differences compared to other members of the phylogenetically related genera in the family <i>Marinifilaceae</i>, strain DW002^T is proposed to represent a novel genus within the family. Therefore, the name <i>Paralabilibaculum antarcticum</i> gen. nov., sp. nov. is proposed. The type strain is DW002^T (=KCTC 25274^T=MCCC 1K06067^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteus faecis: a potentially pathogenic bacterium isolated from the freshwater Yangtze finless porpoise","authors":"Richard William McLaughlin,&nbsp;YaLu Wang,&nbsp;ShuYa Zhang,&nbsp;HaiXia Xie,&nbsp;XiaoLing Wan,&nbsp;Hui Liu,&nbsp;YuJiang Hao,&nbsp;ChaoQun Wang,&nbsp;JinSong Zheng","doi":"10.1007/s10482-024-02023-2","DOIUrl":"10.1007/s10482-024-02023-2","url":null,"abstract":"<div><p><i>Proteus faecis</i> is a gram-negative facultative anaerobic rod-shaped bacterium capable of swarming motility. It has been isolated from numerous sources such as humans, animals, and refuse and is considered potentially pathogenic towards humans. In this study, bacteria were isolated from the blowhole of a <i>Yangtze finless porpoise</i> (<i>Neophocaena asiaeorientalis asiaeorientalis</i>; YFP) living in captivity in China. One bacterium, <i>P. faecis</i> porpoise, was isolated and whole genome sequencing done. Biofilm formation, motility and antimicrobial resistance were also investigated. To find putative virulence factors, the genome of <i>P. faecis</i> strain porpoise was compared to the genomic sequences of eight other <i>P. faecis</i> isolates using the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) (https://www.bv-brc.org/). The goal of this study was to initially characterize the pathogenicity of this bacterium isolated from a cetacean species using both pathogenomics and conventional approaches.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential","authors":"Ling Yao,&nbsp;Guo-Hong Liu,&nbsp;Shu-Yi Zhang,&nbsp;Peng Gao,&nbsp;Christopher Rensing,&nbsp;Qiu-E Yang,&nbsp;Shun-Gui Zhou","doi":"10.1007/s10482-024-02017-0","DOIUrl":"10.1007/s10482-024-02017-0","url":null,"abstract":"<div><p>Two facultatively aerobic strains, designated SGZ-02^T and SGZ-792^T, were isolated from plant <i>Pennisetum</i> sp., exhibiting the highest 16S rRNA gene sequence similarities with the type strains of <i>Sphingomonas zeae</i> LMG 28739^T (98.6%) and <i>Massilia forsythiae</i> NBRC 114511^T (98.4%), respectively. SGZ-02^T grew between 5 and 45 °C, pH 5.0–11.0 and tolerated NaCl concentrations of 0–4% (w/v), whereas SGZ-792^T thrived at 5–40 °C, pH 5.0–11.0 and NaCl tolerance to 0–3.5% (w/v). The major quinone of SGZ-02^T was ubiquinone-10, with the dominant fatty acids being C_16:0 (13.5%), Summed Feature 3 (6.3%), C_14:02-OH (5.3%) and Summed Feature 8 (66.3%). SGZ-792^T predominantly contained ubiquinone-8, with major fatty acids being C_16:0 (20.3%), Summed Feature 3 (5.0%) and Summed Feature 8 (54.7%). Average nucleotide identity and digital DNA–DNA hybridization values between two strains and their closest references strains were below the bacterial species threshold. Based on genotypic and phenotypic characteristics, strains SGZ-02^T and SGZ-792^T are proposed as novel species within the genera <i>Sphingomonas</i> and <i>Massilia</i>, respectively. The suggested names for the new species are <i>Sphingomonas fuzhouensis</i> sp. nov. (SGZ-02^T = GDMCC 1.4033^T = JCM 36769^T) and <i>Massilia phyllosphaerae</i> sp. nov. (SGZ-792^T = GDMCC 1.4211^T = JCM 36643^T), respectively.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two new species of Fusarium in the F. incarnatum-equiseti species complex from Oryza sativa in Iran","authors":"Saeid Afzalinia,&nbsp;Mehdi Mehrabi-Koushki,&nbsp;Reza Farokhinejad","doi":"10.1007/s10482-024-02019-y","DOIUrl":"10.1007/s10482-024-02019-y","url":null,"abstract":"<div><p>Identification of <i>Fusarium</i> species associated with diseases symptoms in plants is an important step toward understanding the ecology of plant–fungus associations. In this study, four <i>Fusarium</i> isolates were obtained from root rot of <i>Oryza sativa</i> L. in Izeh (southwest of Iran) and identified based on phylogenetic analyses combined with morphology. Phylogenetic analyses based on combined translation elongation factor 1-α, calmodulin, RNA polymerase II second largest subunit, and Beta-tubulin (<i>tub2</i>) sequence data delimited two new species, namely <i>F. khuzestanicum</i> and <i>F. oryzicola</i> spp. nov., from previously known species of <i>Fusarium</i> within <i>F. incarnatum</i>-<i>equiseti</i> species complex (FIESC). Morphologically, <i>F. khuzestanicum</i> produces the macroconidia with distinctly notched to foot-shaped basal cells, while basal cells in the macroconidia of <i>F. oryzicola</i> are more extended and distinctly elongated foot shape. Furthermore, these two new species are distinguished by the size of their sporodochial phialides and macroconidia. The results of the present show that the FIESC species complex represent more cryptic species.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yunchengibacter salinarum gen. nov., sp. nov., a novel bacterium of the family Kordiimonadaceae isolated from sediment in Yuncheng salt lake","authors":"Hao-Ran Guo,&nbsp;Fan Wang,&nbsp;Hui-Ying Yu,&nbsp;Chuan-Xu Wang,&nbsp;Zhuo Wang,&nbsp;Bao-Zhu Fang,&nbsp;Xin Li,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-024-02011-6","DOIUrl":"10.1007/s10482-024-02011-6","url":null,"abstract":"<div><p>A Gram-stain-negative, aerobic, motile and rod-shaped bacterium, the color of the bacterial colony ranges from light yellow to yellow, designated YC-2023-2T, was isolated from sediment sample of Yuncheng salt lake. Growth occurred at 15–45℃ (optimum 37℃), pH 6.0-9.0 (optimum pH 7.0-8.0) and with 0–8.0% NaCl (w/v, optimum 2.0%). The phylogenetic analysis based on 16S rRNA gene sequences showed that strain YC-2023-2T belonged to the family Kordiimonadaceae. The closely related members were Gimibacter soli 6D33T (92.38%), Kordiimonas lipolytica M41T (91.88%), Eilatimonas milleporae DSM 25217T (91.88%) and Kordiimonas gwangyangensis JCM 12864T (91.84%). The genome of strain YC-2023-2T was 2957513 bp, and the genomic DNA G+C content was 63.91%. The main respiratory quinone was Q-10 and the major fatty acids (&gt;10%) were iso-C15:0, C16:0, C19:0 cyclo ω8c, Summed Feature 8 (C18:1 ω6c or C18:1 ω7c) and Summed Feature 9 (iso-C17:1 ω9c or C16:0 10-methyl). The major polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified glycolipid, unidentified lipid, and two unidentified aminolipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain YC-2023-2T is proposed to represent a novel species of a novel genus named Yunchengibacter salinarum gen. nov., sp. nov., within the family Kordiimonadaceae. The type strain is YC-2023-2T (= GDMCC 1.4502T = KCTC 8546T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallium: a decisive “Trojan Horse” against microorganisms","authors":"Amanda Stefanie Jabur de Assis,&nbsp;Guilherme Manassés Pegoraro,&nbsp;Iolanda Cristina Silveira Duarte,&nbsp;Tiago Palladino Delforno","doi":"10.1007/s10482-024-02015-2","DOIUrl":"10.1007/s10482-024-02015-2","url":null,"abstract":"<div><p>Controlling multidrug-resistant microorganisms (MRM) has a long history with the extensive and inappropriate use of antibiotics. At the cost of these drugs being scarce, new possibilities have to be explored to inhibit the growth of microorganisms. Thus, metallic compounds have shown to be promising as a viable alternative to contain pathogens resistant to conventional antimicrobials. Gallium (Ga³⁺) can be highlighted, which is an antimicrobial agent capable of disrupting the essential activities of microorganisms, such as metabolism, cellular respiration and DNA synthesis. It was observed that this occurs due to the similar properties between Ga³⁺ and iron (Fe³⁺), which is a fundamental ion for the correct functioning of bacterial activities. The mimetic effect performed by Ga³⁺ prevents iron transporters from distinguishing both ions and results in the substitution of Fe³⁺ for Ga³⁺ and in adverse metabolic disturbances in rapidly growing cells. This review focuses on analyzing the development of research involving Ga³⁺, elucidating the intracellular incorporation of the “Trojan Horse”, summarizing the mechanism of interaction between gallium and iron and comparing the most recent and broad-spectrum studies using gallium-based compounds with antimicrobial scope.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tapping into haloalkaliphilic bacteria for sustainable agriculture in treated wastewater: insights into genomic fitness and environmental adaptation","authors":"Yosra Amara,&nbsp;Mouna Mahjoubi,&nbsp;Yasmine Souissi,&nbsp;Hanene Cherif,&nbsp;Islem Naili,&nbsp;Darine ElHidri,&nbsp;Imen Kadidi,&nbsp;Amor Mosbah,&nbsp;Ahmed S Masmoudi,&nbsp;Ameur Cherif","doi":"10.1007/s10482-024-02012-5","DOIUrl":"10.1007/s10482-024-02012-5","url":null,"abstract":"<div><p>The increasing salinity and alkalinity of soils pose a global challenge, particularly in arid regions such as Tunisia, where about 50% of lands are sensitive to soil salinization. Anthropogenic activities, including the use of treated wastewater (TWW) for irrigation, exacerbate these issues. Haloalkaliphilic bacteria, adapted to TWW conditions and exhibiting plant-growth promotion (PGP) and biocontrol traits, could offer solutions. In this study, 24 haloalkaliphilic bacterial strains were isolated from rhizosphere sample of olive tree irrigated with TWW for more than 20 years. The bacterial identification using 16S rRNA gene sequencing showed that the haloalkaliphilic isolates, capable of thriving in high salinity and alkaline pH, were primarily affiliated to <i>Bacillota</i> (<i>Oceanobacillus</i> and <i>Staphylococcus</i>). Notably, these strains exhibited biofertilization and enzyme production under both normal and saline conditions. Traits such as phosphate solubilization, and the production of exopolysaccharide, siderophore, ammonia, and hydrogen cyanide were observed. The strains also demonstrated enzymatic activities, including protease, amylase, and esterase. Four selected haloalkaliphilic PGPR strains displayed antifungal activity against <i>Alternaria terricola</i>, with three showing tolerances to heavy metals and pesticides. The strain <i>Oceanobacillus picturea</i> M4W.A2 was selected for genome sequencing. Phylogenomic analyses indicated that the extreme environmental conditions probably influenced the development of specific adaptations in M4W.A2 strain, differentiating it from other <i>Oceanobacillus picturae</i> strains. The presence of the key genes associated with plant growth promotion, osmotic and oxidative stress tolerance, antibiotic and heavy metals resistance hinted the functional capabilities might help the strain M4W.A2 to thrive in TWW-irrigated soils. By demonstrating this connection, we aim to improve our understanding of genomic fitness to stressed environments. Moreover, the identification of gene duplication and horizontal gene transfer events through mobile genetic elements allow the comprehension of these adaptation dynamics. This study reveals that haloalkaliphilc bacteria from TWW-irrigated rhizosphere exhibit plant-growth promotion and biocontrol traits, with genomic adaptations enabling their survival in high salinity and alkaline conditions, offering potential solutions for soil salinization issues.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbacterium rhizophilus sp. nov., an indole acetic acid-producing actinobacterium isolated from rhizosphere soil","authors":"Haifei Liu,&nbsp;Quan Yang,&nbsp;Jiawei Li,&nbsp;Lifang Yang,&nbsp;Aolin Zhao,&nbsp;Ying Huang,&nbsp;Hongcun Liu,&nbsp;Shujing Wu,&nbsp;Mingguo Jiang","doi":"10.1007/s10482-024-02014-3","DOIUrl":"10.1007/s10482-024-02014-3","url":null,"abstract":"<div><p>A novel gram-stain-positive, short rod, aerobic, non-motile and non-spore-forming actinobacterial strain, designated GXG1230^T was isolated from the rhizosphere soil of a coastal mangrove forest in Beihai city, Guangxi Zhuang Autonomous Region, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GXG1230^T was affiliated with the genus <i>Microbacterium</i>. Additionally, it demonstrated a high degree of similarity to <i>Microbacterium paludicola</i> US15^T (97.9%) and <i>Microbacterium marinilacus</i> YM11-607^T (97.3%). Chemotaxonomic characteristics showed that the whole-cell sugars were glucose, xylose, rhamnose and galactose. Menaquinones MK-11 and MK-12 were detected as respiratory quinones. Lysine was found in the peptidoglycan hydrolysate and the polar lipids were diphosphatidylglycerol, one phospholipid and two unidentified glycolipid. The major fatty acids were <i>anteiso</i>-C_15:0, <i>iso</i>-C_16:0 and <i>anteiso</i>-C_17:0. The strain GXG1230^T exhibited a genomic DNA G + C content of 71.7%. Furthermore, the average nucleotide identity values of GXG1230^T with the reference strains were 75.4% and 81.9%, respectively, while the digital DNA-DNA hybridization values were 20.1% and 25.0%. Based on physiological, chemotaxonomic and phylogenetic information, strain GXG1230^T is considered to represent a novel species of the genus <i>Microbacterium</i>, for which the name <i>Microbacterium rhizophilus</i> sp.nov is proposed, with GXG1230^T (= MCCC 1K09302^T = KCTC 59252^T) as the type strain.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10482-024-02014-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial community diversity analysis of kiwifruit pollen and identification of potential pathogens","authors":"Jiaqi Yang,&nbsp;Manli Yao,&nbsp;Dan Zhang,&nbsp;Yu Zhao,&nbsp;Guitian Gao","doi":"10.1007/s10482-024-02013-4","DOIUrl":"10.1007/s10482-024-02013-4","url":null,"abstract":"<div><p>The kiwifruit industry typically uses commercial pollen for artificial pollination. However, during the collection of male flowers and pollen production, pollen can be easily contaminated by pathogenic bacteria that cause diseases such as canker and flower rot. Consequently, it is crucial to understand the structure of the pollen microbial community. This study employed Illumina high-throughput sequencing technology to analyze the fungal and bacterial composition in pollen samples from various regions in Shaanxi Province. Concurrently, potential pathogenic strains were isolated using traditional microbial isolation and cultivation techniques, and their molecular identification was performed through 16S rDNA sequence analysis. A tieback test was conducted on healthy branches to verify the pathogenicity of the strains. The results revealed a rich diversity of fungi and bacteria in kiwifruit pollen. At the phylum level, pollen fungi were mainly distributed in Ascomycota, and bacteria were mainly distributed in Proteobacteria and Firmicutes. The dominant fungal genera were <i>Mycosphaerella</i>, <i>Aspergillus</i>, and <i>Cladosporium;</i> the dominant bacterial genera were <i>Weissella</i>, <i>Pantoea</i>, <i>Enterobacter</i>, and <i>Pseudomonas</i>, respectively. Additionally, both <i>Erwinia persicina</i> and <i>Pseudomonas fluorescens</i>, isolated from pollen, exhibited high pathogenicity toward healthy kiwifruit branches. These findings contribute to a deeper understanding of the microbial diversity in commercial kiwifruit pollen used for mass pollination.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142009918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}