Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Parasalinivibrio latis gen. nov., sp. nov., isolated from the distal gut of healthy farmed Asian Seabass (Lates calcarifer)","authors":"Shuan Er,&nbsp;Melissa Soh,&nbsp;Adrian Low,&nbsp;Henning Seedorf","doi":"10.1007/s10482-024-02036-x","DOIUrl":"10.1007/s10482-024-02036-x","url":null,"abstract":"<div><p>Asian Seabass (<i>Lates calcarifer</i>) is widely farmed as a sustainable source of protein for countries in the tropical Indo-West Pacific region. However, microbial species of the gut microbiome of healthy Asian Seabass remain largely uncharacterized and uncultured. Here, we analysed the microbial composition along the gastrointestinal tract of a farmed healthy Asian Seabass. We used different cultivation approaches to obtain isolates from the seabass intestinal tract and describe the isolation and characterization of a novel strain, TLL-SE01^T. Analysis of the strain’s 16S rRNA gene indicates that the strain belongs to the family <i>Vibrionaceae</i> with <i>Photobacterium damselae</i> as its closest relative, albeit sharing only 94.8% (aligned region 1553 bp) nucleotide identity. Comparative genomic analysis with all validly published <i>Vibrionaceae</i> species with available genomes revealed average nucleotide identity (ANI) and DNA–DNA hybridisation (DDH) values of around 70% and 24% respectively to strain TLL-SE01^T, which are well below proposed thresholds for species delineation (ANI, 95–96%; DDH, 70%). The alignment fraction and ANI genus demarcation boundaries for all genera in the <i>Vibrionaceae</i> family were determined for which strain TLL-SE01^T is well below the calculated values, indicating that it belongs to a novel genus. Single- and core-gene phylogenetic analysis places strain TLL-SE01^T in a monophyletic clade, further supporting its designation to a novel genus. Phenotypic comparison between strain TLL-SE01^T and its close relatives indicated additional differences, such as growth response at different salt concentrations and different metabolic capabilities. Based on genotypic, phylogenetic and phenotypic differences to other <i>Vibrionaceae</i> species, we propose a novel species in a new genus, <i>Parasalinivibrio latis</i> gen. nov. sp. nov. and strain TLL-SE01^T (= BCRC 81435^T = JCM 36283^T) as the type strain.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142596097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid","authors":"Hong-Chuan Wang,&nbsp;Meng-Han Huang,&nbsp;Dan-Yuan Guo,&nbsp;Wen-Li Liu,&nbsp;Zi-Wen Yang,&nbsp;Dao-Feng Zhang,&nbsp;Wen-Jun Li","doi":"10.1007/s10482-024-02034-z","DOIUrl":"10.1007/s10482-024-02034-z","url":null,"abstract":"<div><p>Strain WL0113^T was isolated from surface seawater of the coast of Lianyungang, Jiangsu province, PR China. Strain WL0113^T shared highest 16S rRNA gene sequence similarity with <i>Roseobacter insulae</i> YSTF-M11^T (98.8%), followed by <i>R. cerasinus</i> AI77^T (98.8%), <i>R</i>. <i>ponti</i> MM-7^T (98.0%). Strain WL0113^T was Gram-stain-negative, cream, aerobic, non-motile and coccoid- to oval-shaped, and able to grow at pH 6.5–9.0 (optimum, pH 7.0–8.0), at 10–37  °C (optimum, 28 °C) and in the presence of 1–5% (w/v; optimum, 2.5%) NaCl. Ubiquinone-10 was detected as dominant. The main fatty acids (&gt; 5%) of the strain WL0113^T were C_16:0, iso-C_17:0 3OH, C_20:4<i>ω</i>6,9,12,15<i>c</i> (arachidonic acid), and summed feature 8 (C_18:1<i>ω</i>7<i>c</i> and/or C_18:1<i>ω</i>6<i>c</i>). The major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, glycophospholipid, unknown aminolipid, unknown phospholipid, and two unknown polar lipids. The ANI and dDDH values between strain WL0113^T and <i>Roseobacter cerasinus</i> were 80.4% and 23.0%, respectively. The genomic DNA G + C content of strain WL0113^T was 63.1%. Based on these data, it is proposed that strain WL0113^T represent novel species of the genus <i>Roseobacter</i>, for which the name <i>Roseobacter sinensis</i> sp. nov. is proposed. The type strain is WL0113^T (= GDMCC 1.3082^T = JCM 35567^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Separation and purification of antimicrobial substances from Paenibacillus polymyxa KH-19 and analysis of its physicochemical characterization","authors":"Longtao Dou,&nbsp;Wei Liu,&nbsp;Jihua Hu,&nbsp;Shumei Zhang,&nbsp;Xianghui Kong,&nbsp;Xiaojun Qu,&nbsp;Wei Jiang","doi":"10.1007/s10482-024-02029-w","DOIUrl":"10.1007/s10482-024-02029-w","url":null,"abstract":"<div><p>Soft rot is one of the top ten most dangerous plant pathogens in agricultural production, storage, and transport, and the use of microorganisms and their metabolites to control soft rot is a current research hotspot. In this study, we identified the antimicrobial substance in the metabolite of <i>Paenibacillus polymyxa</i> KH-19, and determined that the antimicrobial substance of this strain was an active protein. The protein was completely precipitated at 40–60% ammonium sulphate saturation and showed good inhibitory effects against seven pathogenic bacteria including <i>Pectobacterium carotovorum</i> BC2 and seven pathogenic fungi including <i>Pyricularia oryzae</i>. The MIC of the protein was 51.563 µg/mL, temperature acid–base UV and light stability insensitive to protease, with high-temperature resistance. The antimicrobial protein was isolated and purified by DEAE-anion exchange column and Sephadex G-75 gel filtration chromatography, and the LC–MS/MS assay identified the protein as lysophosphatidyl esterase with a molecular weight of 25.255 kDa. The purified antimicrobial protein increased the inhibitory effect against <i>P. carotovorum</i> BC2, with the diameter of the circle of inhibition being 26.50 ± 0.915 mm. Bioinformatics analysis showed that the protein has the molecular formula of C₁₁₁₇H₁₇₃₂N₃₁₆O₃₃₈S₅, encodes 224 amino acids, has an aliphatic index of 88.39, and belongs to the category of hydrophilic unstable proteins. The present study is the first report of an active protein with extreme thermoplastic and resistance to <i>P. carotovorum</i> BC2, which provides a reference for the preparation and application of the antimicrobial substances of <i>P. polymyxa</i> KH-19, as well as a theoretical basis for the study of the function of lysophosphodiesterase protein and its use as a microbial preparation.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomic analyses of the genus Robertmurraya and proposal of the novel species Robertmurraya mangrovi sp. nov., isolated from mangrove soil","authors":"Ming Li,&nbsp;Xixi Hu,&nbsp;Tiancheng Ni,&nbsp;Yuan Ni,&nbsp;Dong Xue,&nbsp;Feng Li","doi":"10.1007/s10482-024-02032-1","DOIUrl":"10.1007/s10482-024-02032-1","url":null,"abstract":"<div><p>A Gram-positive, aerobic, motile, rod-shaped bacterial strain, designated 31A1R^T, was isolated from the mangrove soil of Xilian village, Zhanjiang, China. Strain 31A1R^T thrives at temperatures ranging from 15 to 45 °C (optimum at 30 °C), pH 6.5–10 (optimum at 8.5), and in the presence of 0–5% (w/v) NaCl (optimum at 1.5%). The strain shares the highest 16S rRNA gene sequence similarity with <i>Robertmurraya crescens</i> (97.24%) and <i>Robertmurraya dakarensis</i> (97.18%). The complete genome of strain 31A1R^T spans 4.71 Mbp with a genomic DNA G + C content of 35.9 mol%. The average nucleotide identity and DNA–DNA hybridization values between strain 31A1R^T and type strains of other species of the genus <i>Robertmurraya</i> were 71.24–72.11% and 19.90–21.40%, respectively. The amino acid identity values and percentage of conserved proteins ranged from 66.94 to 68.10% and from 58.34 to 61.62%, respectively, aligning with intrageneric cutoff values. The major fatty acids (≥ 5.0%) were iso-C_14:0 (5.0%), iso-C_15:0 (41.4%), iso-C_16:0 (12.6%), C_16:1ω7c alcohol (12.2%), and iso-C_17:1 ω10c (6.5%). The polar lipids profile was mainly composed of diphosphatidyl glycerol, phosphatidyl glycerol, and phosphatidyl ethanolamine. We also profiled the pan-genome and metabolic features of genomic assemblies of strains belonging to the genus <i>Robertmurraya</i>, which indicated functional capacities and metabolic similarities. Consequently, we propose that strain 31A1R^T represents a new species in the genus <i>Robertmurraya</i>, for which the name <i>Robertmurraya mangrovi</i> sp. nov. is proposed, with the type strain being 31A1R^T (= GDMCC 1.4378^T = JCM 36937^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142512373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omics exploration of Tetraselmis chuii adaptations to diverse light regimes","authors":"Maria Patelou,&nbsp;Aikaterini Koletti,&nbsp;Carlos Infante,&nbsp;Dimitrios Skliros,&nbsp;Fotios Komaitis,&nbsp;Chrysanthi Kalloniati,&nbsp;Eleni Tsiplakou,&nbsp;Alexandros Mavrommatis,&nbsp;Lalia Mantecón,&nbsp;Emmanouil Flemetakis","doi":"10.1007/s10482-024-02028-x","DOIUrl":"10.1007/s10482-024-02028-x","url":null,"abstract":"<div><p>Microalgae are significantly influenced by light quality and quantity, whether in their natural habitats or under laboratory and industrial culture conditions. The present study examines the adaptive responses of the marine microalga <i>Tetraselmis chuii</i> to different light regimes, using a cost-effective filtering method and a multi-omics approach. Microalgal growth rates were negatively affected by all filtered light regimes. After six days of cultivation, growth rate for cultures exposed to blue and green filtered light was 67%, while for red filter was 83%, compared to control cultures. Transcriptomic analysis revealed that the usage of green filters resulted in upregulation of transcripts involved in ribosome biogenesis or coding for elongation factors, exemplified by a 2.3-fold increase of <i>TEF3</i>. On the other hand, a 2.7-fold downregulation was observed in photosynthesis-related <i>petJ</i>. Exposure to blue filtered light led to the upregulation of transcripts associated with pyruvate metabolism, while photosynthesis was negatively impacted. In contrast, application of red filter induced minor transcriptomic alterations. Regarding metabolomic analysis, sugars, amino acids, and organic acids exhibited significant changes under different light regimes. For instance, under blue filtered light sucrose accumulated over 6-fold, while aspartic acid content decreased by 4.3-fold. Lipidomics analysis showed significant accumulation of heptadecanoic and linoleic acids under green and red light filters. Together, our findings indicate that filter light can be used for targeted metabolic manipulation.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142447305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Enterocin B3A-B3B produced by LAB collected from infant faeces: potential utilization in the food industry for Listeria monocytogenes biofilm management","authors":"Alaa Al-Seraih,&nbsp;Yanath Belguesmia,&nbsp;John Baah,&nbsp;Sabine Szunerits,&nbsp;Rabah Boukherroub,&nbsp;Djamel Drider","doi":"10.1007/s10482-024-02005-4","DOIUrl":"10.1007/s10482-024-02005-4","url":null,"abstract":"","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brevibacterium litoralis sp. nov., a cellulose-degrading strain isolated from marine surface sediment","authors":"Quan Yang,&nbsp;Aolin Zhao,&nbsp;Haifei Liu,&nbsp;Jiawei Li,&nbsp;Shujing Wu,&nbsp;Ying Huang,&nbsp;Jie Weng,&nbsp;Mingguo Jiang,&nbsp;Yi Jiang","doi":"10.1007/s10482-024-02031-2","DOIUrl":"10.1007/s10482-024-02031-2","url":null,"abstract":"<div><p>A Gram stain-positive, non-spore-forming, non-motile, short-rod actinomyces strain GXQ1321^T was isolated from maritime surface sediments in Beihai (21° 41′ 21.65″ N, 109° 05′ 76.56″ E), Guangxi Zhuang Autonomous Region, and a number of categorization studies were performed. Following a period of 72 h of incubation at a temperature of 30 °C within a modified actinomycete culture medium, the colony was light-yellow, circular, smooth, central bulge, convex, opaque, with a 1.2–2.3 mm diameter. Strain GXQ1321^T had the ability to degrade cellulose. Chemotaxonomic studies revealed that the major methylnaphthoquinones in strain GXQ1321^T was MK-8(H₂). The most prevalent cellular fatty acids were <i>anteiso</i>-C_19:0, <i>anteiso</i>-C_15:0, <i>anteiso</i>-C_17:0, and <i>iso</i>-C_16:0. The whole-cell sugars of the strain GXQ1321^T were identified rhamnose, xylose and glucose. Meso-diaminopimelic acid was found in the peptidoglycan hydrolysate, and the polar lipids were identified as diphosphatidylglycerol, three phosphoglycolipid, phosphatidylglycerol and two unknown glycolipid. This strain had 69.6% DNA G+C content. Strain GXQ1321^T is classified as <i>Brevibacterium</i> based on its 16S rRNA gene sequence. It is closely related to <i>Brevibacterium samyangense</i> SST-8^T (96.8%) and <i>Brevibacterium rongguiense</i> 5221^T (96.3%). The average nucleotide identity (ANI) values of GXQ1321^T and the above two type strains were 73.9–77.1%, and the digital DNA-DNA hybridisation (dDDH) values were 15.3–21.1%. Based on the phylogenetic, chemotaxonomic and physiological data, strain GXQ1321^T was considered to be a novel species of the genus <i>Brevibacterium</i>, named <i>Brevibacterium litoralis</i> sp. nov, with the type strain GXQ1321^T (= MCCC 1K08964^T = KCTC 59167^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomic analyses of the Listeriaceae family support species reclassification and proposal of a new family and new genera","authors":"Khaoula Bouznada,&nbsp;Hadj Ahmed Belaouni,&nbsp;Rafika Saker,&nbsp;Fawzia Chaabane Chaouch,&nbsp;Atika Meklat","doi":"10.1007/s10482-024-02027-y","DOIUrl":"10.1007/s10482-024-02027-y","url":null,"abstract":"<div><p>The taxonomy of the <i>Listeriaceae</i> family has undergone substantial revisions, expanding the <i>Listeria</i> genus from 6 to 29 species since 2009. However, these classifications have relied on 16S rRNA gene sequences and conventional polyphasic taxonomy, with limited use of genomic approaches. This study aimed to employ genomic tools, including phylogenomics, Overall Genomic Relatedness Indices (OGRIs), and core-genome phylogenomic analyses, to reevaluate the taxonomy of the <i>Listeriaceae</i> family. The analyses involved the construction of phylogenetic and phylogenomic trees based on 16S rRNA gene sequences and core genomes from 34 type strain genomes belonging to <i>Listeriaceae</i> family. OGRIs, which encompass Average Amino acid Identity (AAI), core-proteome AAI (cAAI), and Percentage of Conserved Proteins (POCP), were calculated, and specific threshold values of 70%, 87%, and 72–73% were established, respectively, to delimitate genera in the <i>Listeriaceae</i> family. These newly proposed OGRI thresholds unveiled distinct evolutionary lineages. The outcomes of this taxonomic re-evaluation were: (i): the division of the <i>Listeria</i> genus into an emended <i>Listeria</i> genus regrouping only <i>Listeria senso stricto</i> species; (ii): the remaining <i>Listeria senso lato</i> species were transferred into three newly proposed genera: <i>Murraya</i> gen. nov., <i>Mesolisteria</i> gen. nov., and <i>Paenilisteria</i> gen. nov. within <i>Listeriaceae</i>; (iii): <i>Brochothrix</i> was transferred to the newly proposed family <i>Brochothricaceae</i> fam. nov. within the <i>Caryophanales</i> order; (iiii): <i>Listeria ivanovii</i> subsp. <i>londonensis</i> was elevated to the species level as <i>Listeria londonensis</i> sp. nov.; and (iiiii): <i>Murraya murrayi</i> comb. nov. was reclassified as a later heterotypic synonym of <i>Murraya grayi</i> comb. nov. This taxonomic framework enables more precise identification of pathogenic <i>Listeriaceae</i> species, with significant implications for important areas such as food safety, clinical diagnostics, epidemiology, and public health.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterovibrio qingdaonensis sp. nov. and Enterovibrio gelatinilyticus sp. nov., two marine bacteria isolated from surface seawater of Qingdao offshore","authors":"Keyi Huang,&nbsp;Yi Liu,&nbsp;Yan Wang,&nbsp;Pingping Zhang,&nbsp;Haojin Cheng,&nbsp;Jinfeng Wang,&nbsp;Wanjia Tong,&nbsp;Wang Xiaolei,&nbsp;Xiao-Hua Zhang","doi":"10.1007/s10482-024-02030-3","DOIUrl":"10.1007/s10482-024-02030-3","url":null,"abstract":"<div><p>Two Gram-stain-negative, facultatively anaerobic, motile, and rod-shaped marine bacteria, designated strains ZSDZ35^T and ZSDZ42^T, were isolated from surface seawater of Qingdao offshore. Phylogenetic analysis of the 16S rRNA genes and whole genome data placed ZSDZ35^T and ZSDZ42^T within the genus <i>Enterovibrio</i>. Strain ZSDZ35^T was most closely related to <i>Enterovibrio nigricans</i> DSM 22720^T with 97.55% sequence similarity, whereas ZSDZ42^T was most closely related to <i>Enterovibrio calviensis</i> DSM 14347^T with 98.97% sequence similarity. Strain ZSDZ35^T grew with 0–8% (w/v) NaCl (optimum 4%), at 16–37 °C (optimum 28 °C) and pH 6.0–9.0 (optimum pH 8.0); whereas strain ZSDZ42^T grew with 1–7% (w/v) NaCl (5%), at 4–28 °C (8 °C) and pH 6.0–9.0 (pH 7.0). Both strains shared the same major fatty acid components of summed feature 3 (C_16:1ω7c or/and C_16:1ω6c), summed feature 8 (C_18:1ω7c and C_18:1ω6c) and C_16:0, with different proportions. The DNA G + C contents of strains ZSDZ35^T and ZSDZ42^T were 47.2% and 46.7%, respectively. Based on the results of polyphasic analyses, ZSDZ35^T and ZSDZ42^T are considered to represent novel species, for which the names <i>Enterovibrio qingdaonensis</i> sp. nov. (type strain, ZSDZ35^T = MCCC 1K06293^T = KCTC 82887^T) and <i>Enterovibrio gelatinilyticus</i> sp. nov. (type strain, ZSDZ42^T = MCCC 1K06294^T = KCTC 82886^T) are proposed, respectively.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of meningitis-causing bacteria, with focus on genomic and pangenomic study of multi-drug resistant Streptococcus pneumoniae from cerebrospinal fluid","authors":"Rehan Ali,&nbsp;Kashif Ali,&nbsp;Muhammad Aurongzeb,&nbsp;Khalid Al-Regaiey,&nbsp;Junaid Ahmed Kori,&nbsp;Muhammad Irfan,&nbsp;Yasmeen Rashid,&nbsp;Danah Al Abduljabbar,&nbsp;Imdad Kaleem,&nbsp;Shahid Bashir","doi":"10.1007/s10482-024-02016-1","DOIUrl":"10.1007/s10482-024-02016-1","url":null,"abstract":"<div><p><i>Streptococcus pneumoniae</i> is a major cause of meningitis in under developed countries with low vaccination rates and high antibiotic resistance. This study aimed to analyze 83 suspected meningitis patients in Karachi for the detection of <i>S. pneumoniae followed by its</i> whole genome sequencing and Pan Genome analysis<i>.</i> Out of the 83 samples collected, 33 samples with altered physical (turbidity), cytological (white blood cell count) and biochemical (total protein and total glucose concentrations) parameters indicated potential meningitis cases, while these parameters were within normal healthy ranges in remaining 50 samples. Latex particle agglutination (LPA) was performed on the 33 samples, revealing 20 positive cases of bacterial meningitis. The PCR and culturing methods revealed 5 <i>S. pneumoniae</i> isolates. Antibiotic susceptibility tests showed that one <i>S</i><i>.</i> <i>pneumoniae</i> strain was resistant to erythromycin, levofloxacin, and tetracycline. Whole-genome sequencing of this resistant strain was performed and <i>S. pneumoniae</i> was confirmed with MLST analysis, while it had &gt; 2.3 Mb genome and a single repUS43 plasmid. In CARD analysis, the strain had tet(M), ermB, RlmA(II), patB, pmrA, and patA ARGs, which could provide resistance against tetracycline, macrolide, fluoroquinolone, and glycopeptide antibiotics. Phylogenetic analysis revealed that the isolate was closely related to strains from Hungary and the USA. Pan-genome analysis with 144 genome assemblies from NCBI database showed that 1101 non-redundant core genes were shared between all strains. This study gives valuable understanding into the prevalence and characterization of meningitis-causing bacteria in Karachi, Pakistan  with prime focus on multi-drug resistant <i>S. pneumoniae</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142394982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}