Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献

{"title":"Biological control of Aspergillus flavus in peanut and maize kernel using Pantoea vagans BWL1","authors":"Danfeng Zhang,&nbsp;Xinrui Tian,&nbsp;Tingting Hu,&nbsp;Yanqiao Wang,&nbsp;Yonghai Wang,&nbsp;Changqing Hong,&nbsp;Wei Shi,&nbsp;Yingwang Ye","doi":"10.1007/s10482-025-02154-0","DOIUrl":"10.1007/s10482-025-02154-0","url":null,"abstract":"<div><p><i>Aspergillus flavus</i> infenction of crops, which are common worldwide, are a significant threat to human and animal health. In this study, the antagonistic effects of <i>Pantoea vagans</i> strain BWL1 on <i>A. flavus</i> were analyzed. BWL1 effectively inhibited <i>A. flavus</i>conidial germination, vegetative growth, and sporulation. Notably, <i>A. flavus</i> infections and aflatoxin contents decreased in peanut and maize samples treated with BWL1. A GC–MS analysis and a two-sealed-base-plates assay showed that BWL1 can produce several antifungal volatiles, including 2,5-dimethyl-pyrazine, 2-ethyl-1-hexanol, hexamethyl-cyclotrisiloxane, phenylethyl alcohol, 2,4-di-tert-butylphenol, pentadecane, tetradecane, heptadecane, and n-hexadecanoic acid, with MICs of 0.2, 0.8, 16, 0.8, 0.2, 8, 8, 8, and 8 g/L, respectively. Among these volatiles, 2,5-dimethyl-pyrazine, 2-ethyl-1-hexanol, phenylethyl alcohol, and 2,4-di-tert-butylphenol had substantial inhibitory effects on <i>A. flavus</i> infection of peanuts and maize kernels. The study findings suggest that <i>P. vagans</i> BWL1 may be an potential biocontrol agent for the postharvest management of <i>A. flavus</i> in crops. This is the first report regarding the antifungal effects of <i>P. vagans</i> BWL1 on <i>A. flavus</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Curtobacterium salicis sp. nov., isolated from willow tree stems in Washington state","authors":"John Freeman,&nbsp;Andrea Firrincieli,&nbsp;Douglas Baker,&nbsp;Sharon Doty","doi":"10.1007/s10482-025-02151-3","DOIUrl":"10.1007/s10482-025-02151-3","url":null,"abstract":"","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprotective potential of biosynthesized copper oxide nanoparticles and copper phosphite against Alternaria-solani-induced leaf spot in pepper plants","authors":"Mohamed S. Attia,&nbsp;Mohamed H. Moustafa,&nbsp;Amr H. Hashem,&nbsp;Salah M. Elsayed,&nbsp;Abeer S. Aloufi,&nbsp;Ismail Mostafa Ismail Abdelaleem,&nbsp;Karim A. Alshahed,&nbsp;Abdelrhman S. Ismail,&nbsp;Abdelrhman M. Ibrahim,&nbsp;Mostafa A. Abdel-Maksoud,&nbsp;Abdulaziz Alamri,&nbsp;Hossam Ebaid,&nbsp;Bushra Hafeez Kiani,&nbsp;Hanan El Bakkali,&nbsp;Amer M. Abdelaziz","doi":"10.1007/s10482-025-02143-3","DOIUrl":"10.1007/s10482-025-02143-3","url":null,"abstract":"<div><p><i>Alternaria solani</i> leaf spot disease (ASLS) poses a serious threat to global crop production, including peppers, resulting in notable economic losses. Bio-nanotechnology offers promising solutions for combating plant pathogens by promoting plant defenses and inhibiting pathogen growth. This study explores the effectiveness of copper oxide nanoparticles (CNPs) and copper phosphite (MAXIFOS CU®) in controlling <i>A. solani</i> and boosting growth and defense responses in pepper plants. CNPs were biosynthesized using <i>Penicillium expansum</i> first time and thoroughly characterized through various techniques. Analysis confirmed that the nanoparticles varied in shape, predominantly oval and spherical, with an average size of approximately 40.59 nm, as shown in HR-TEM images. DLS analysis indicated a mean particle size of 74.58 nm, and Zeta potential analysis at pH 7.2 revealed a negative surface charge of − 55.25 mV, attributed to the components of the fungal extract. The study demonstrated that both CNPs and MAXIFOS CU® exhibited antifungal activity against <i>A. solani</i>, with CNPs effectively reducing PDI by 27.5% and enhancing overall plant protection by 65.62%. Results indicated that treated plants showed improvements in photosynthetic pigments, proline content (MAXIFOS CU® 28 g/L increase by 108.3%, while CNPs 81.30%), total phenolic compounds (CNPs 80.70% increase, while MAXIFOS CU® a 68.70%), H₂O₂ levels (MAXIFOS CU® decreased 22%, whereas CNPs 9%), MDA concentration (CNPs 39% decrease in MDA, while MAXIFOS CU® 34%), and the activities of POD (increased by 53.4, and 45.1% CNPs and MAXIFOS CU®) and PPO (CNPs and MAXIFOS CU® increased by 42.8 and 31.6%) enzymes. These findings highlight the potential of an eco-friendly, dual approach using biosynthesized CNPs and Cu-phosphite for managing <i>A. solani</i> and enhancing pepper plant health.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Desulfosporosinus sediminicola sp. nov., an acidophilic sulfate-reducing bacterium isolated from acidic sediments of a disused iron mine site","authors":"Sheng-Fang Jiang,&nbsp;Chu-Yi Zhang,&nbsp;Xuan-Wen Lao,&nbsp;Wei-Chao Jia,&nbsp;Tao-Tao Yang,&nbsp;Si-Yu Zhang,&nbsp;Ying-Ying Xie,&nbsp;Pei-Zhe Ye,&nbsp;Rui Lan,&nbsp;Zi-Xiang Lai,&nbsp;Qiang Yang,&nbsp;Li-Nan Huang","doi":"10.1007/s10482-025-02152-2","DOIUrl":"10.1007/s10482-025-02152-2","url":null,"abstract":"<div><p>An anaerobic, Gram-stain-positive and spore-forming acidophilic sulfate-reducing bacterium, designated as SYSU MS00001^T, was isolated from acidic sediments of Zhongshan Iron Mine, P.R. China. The strain was straight-rod-shaped and motile, oxidase-negative and catalase-negative, with circular, convex, regular-edged and black-pigmented colonies (1–3 mm in diameter) on the solid basal salts/yeast extract plate. Growth and proliferation occurred at 10–40 °C (optimal: 30 °C), pH 3.5–7.5 (optimal: 5.0–5.5) and NaCl concentration of 0–1.6% (optimal: 0.2%), with a doubling time of 8.2 h under the optimal conditions. The strain utilised H₂/CO₂, organic acids (fumarate, citrate, pyruvate, malate, acetate, propionate, lactate, butyrate), alcohols (glycerol), and sugars (fructose, glucose, xylose) as electron donors for sulfate reduction. Sulfate, sulfur, sulfite, thiosulfate, fumarate and nitrate were used as electron acceptors in the presence of glycerol. It also fermented ethanol and methanol without sulfate. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipids, unidentified aminophospholipid, unidentified lipids and unidentified glycolipids. The major fatty acids (&gt; 5%) were C_14:0, C_16:0 and summed features 3 (C_16:1<i>ω</i>7<i>c</i>/C_16:1<i>ω</i>6<i>c</i>). The respiratory quinones identified were MK-7 and MK-8(H₄). Phylogenetic analyses based on 16S rRNA gene and genome sequences indicated that the isolated strain should be assigned to the genus <i>Desulfosporosinus</i>, and the 16S rRNA gene sequence was most closely related to <i>Desulfosporosinus acididurans</i> M1^T with similarity of 98.34%. The genomic DNA G + C content of SYSU MS00001^T was 41.7%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between SYSU MS00001^T and <i>D. acididurans</i> M1^T were 50.6% and 90.95%, respectively. On the basis of phenotypic, phylogenetic and genetic characteristics, strain SYSU MS00001^T represents a novel species within the genus <i>Desulfosporosinus</i>, for which the name <i>Desulfosporosinus sediminicola</i> sp. nov. is proposed. The type strain is SYSU MS00001^T (= GDMCC 1.4736^T = KCTC 25900^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144914780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring enzymatically active bacterial diversity in decomposing cow manure: insights into biodegradation dynamics within the initial mesophilic phase","authors":"Sareh Hatamzadeh,&nbsp;Nima Akbari Oghaz,&nbsp;Zeinab Zare Rahmatabad","doi":"10.1007/s10482-025-02155-z","DOIUrl":"10.1007/s10482-025-02155-z","url":null,"abstract":"<div><p>This study investigated the isolation and characterization of bacterial strains with enzymatic potential from cow manure undergoing a 6-day decomposition process (initial mesophilic heating). A total of 270 bacterial isolates were obtained, with 48 isolates exhibited ligninase, amylase, protease, lipase, cellulase, and xylanase activity. Morphological, biochemical and molecular characterization classified them into nine species. The enzymatic analysis revealed that <i>Bacillus licheniformis</i> exhibited the highest enzymatic activity for ligninase, lipase, and amylase, recording 1.13, 9.92, and 8.1 U/mL, respectively, compared to other bacterial species. Furthermore, <i>B. subtilis</i> exhibited the highest enzymatic activity for protease, xylanase, and cellulase, recording 3.33, 1.96, and 0.5 U/mL, respectively. Our study reported <i>Acetobacter tropicalis</i> and <i>A. pasteurianus</i> with enzymatic activity for lipase and amylase. We also identified <i>Lactiplantibacillus plantarum</i> with enzymatic activity for ligninase and xylanase, and <i>Lacticaseibacillus</i> <i>casei</i> with enzymatic activity for ligninase, cellulase, and xylanase. Additionally, we reported <i>Lentilactobacillus buchneri</i> with enzymatic activity for ligninase, amylase, protease, lipase, cellulase, and xylanase. Furthermore, our study identified <i>Cereibacter sphaeroides</i> with enzymatic activity for cellulase, xylanase, protease, and ligninase, and <i>Streptomyces albidoflavus</i> with enzymatic activity for ligninase. These findings expand the understanding of bacterial enzymatic capabilities with potential in biotechnology, waste degradation, and industrial enzyme production.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue virus: structure, genome, evolution and challenges to control and prevent transmission","authors":"Khalida Umar,&nbsp;Tanisha Sutradhar,&nbsp;P. Prakash,&nbsp;M. Bavanilatha,&nbsp;A. U. Hemamalani,&nbsp;R. Subha Prakashini,&nbsp;T. Thangam,&nbsp;Krupakar Parthasarathy","doi":"10.1007/s10482-025-02153-1","DOIUrl":"10.1007/s10482-025-02153-1","url":null,"abstract":"<div><p>Dengue virus (DENV) is a major global health threat, primarily transmitted by Aedes mosquitoes. It manifests in mild to severe forms, including dengue hemorrhagic fever and dengue shock syndrome, causing significant morbidity and mortality. With four serotypes (DENV-1 to DENV-4), the virus exhibits rapid genetic evolution, complicating vaccine development and disease control. This review explores the structural and genomic characteristics of DENV, emphasizing its evolutionary pressures, immune evasion mechanisms, and emerging strains. The virus’s adaptation to environmental and host factors has led to increased outbreaks, notably in tropical regions. Global warming and urbanization have exacerbated the spread, challenging current vector control strategies. Laboratory diagnosis remains complex, relying on molecular and serological techniques with varying sensitivity. The lack of effective antiviral drugs and universally protective vaccines highlights critical gaps in disease management. Ongoing genomic surveillance and integrated control strategies are crucial for mitigating the impact of new DENV variants. This review highlights the importance of investigating the effect of emerging dengue strains on society, as well as how environmental factors exacerbate their severity.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The metabolic pathway of THF-degrading composite bacteria and its immobilized microspheres","authors":"Kanghong Zhou,&nbsp;Youhong Zhang,&nbsp;Guohong Zhou,&nbsp;Wei Wei","doi":"10.1007/s10482-025-02140-6","DOIUrl":"10.1007/s10482-025-02140-6","url":null,"abstract":"<div><p>Tetrahydrofuran (THF), as a typical recalcitrant organic pollutant, poses a serious threat to ecological security and human health due to its environmental persistence. This study aimed to systematically elucidate the metabolic pathway of THF degradation by efficient composite bacteria and develop immobilized enhancement technology to improve their degradation performance. First, the key metabolic pathway for THF degradation by the composite bacteria was analyzed using GC–MS. Second, sodium alginate-chitosan microspheres encapsulating the composite bacteria were prepared, and the preparation process parameters were systematically optimized through single-factor experiments and Box-Behnken response surface methodology. Metabolic pathway analysis revealed that THF undergoes hydroxylation-induced ring-opening catalyzed by monooxygenase, yielding 4-hydroxybutanal, which is subsequently oxidized to 4-hydroxybutyric acid, and ultimately mineralized to CO₂ and H₂O. Under varying THF initial concentrations (180–540 mg/L) and temperatures (25–40 °C), the immobilized composite bacteria demonstrated significantly higher degradation capability and environmental adaptability compared to free bacteria, with markedly improved degradation efficiency. Furthermore, the immobilized microspheres exhibited excellent reusability, maintaining efficient THF removal rates after 5 consecutive cycles. This research elucidated the metabolic mechanism of THF degradation by the composite bacteria and developed a highly efficient and stable preparation process for the immobilized bacterial agent.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into fungal-bacterial synergy for DDT biotransformation","authors":"Abdulmoseen Segun Giwa,&nbsp;Mahnoor Waheed,&nbsp;Hassan Vaqas Khalid,&nbsp;Ehtisham Shafique,&nbsp;Saleem Ur Rahman,&nbsp;Nasir Ali","doi":"10.1007/s10482-025-02149-x","DOIUrl":"10.1007/s10482-025-02149-x","url":null,"abstract":"&lt;div&gt;&lt;p&gt;Pesticides comprise a diverse group of chemical agents designed to suppress, repel, or eradicate deleterious biological organisms—including phytopathogens, insect pests, and competing flora—that pose a threat to agricultural yields, ornamental plant integrity, and public health. Escalating reliance on these compounds, particularly in low- and middle-income nations, has raised critical concerns within the scientific and public health domains due to emerging evidence linking chronic exposure to a range of adverse health outcomes. Dichlorodiphenyltrichloroethane (DDT) is a widely used pesticide known for its persistence in the environment. The widely used DDT was heavily restricted in the United States due to its unwanted and hazardous effects on human health, wildlife, and the environment at the same time. Similarly, another insecticide, neonicotinoids, was restricted in the European Union because of its involvement in the decline of honeybee populations in the early twenty-first century. To remove such kind of chemicals from contaminated soil and water, various physical, chemical, and biological approaches have been applied. The most cost-effective and eco-friendly strategy to combat pollutants, in which the catabolic capabilities of a fungal-bacterial consortium are exploited for the purpose. Fungal-bacterial interactions play a synergistic role in the degradation of DDT through complementary metabolic capabilities and environmental changes. White rot fungi secrete non-specific extracellular oxidases, manganese peroxidase, and laccase, to initiate the breakdown of the complex aromatic ring of DDT into intermediate metabolites. These fungal activities usually result in the partial conversion of DDT into compounds such as DDD, DDE, or dichlorodiphenylacetone. Subsequently, the associated bacteria (which usually coexist within the mycelium) will utilize specific intracellular enzymes to further metabolize these intermediates through dechlorination, hydroxylation, or ring-opening pathways. In addition, fungi can alter the microenvironment by reducing the redox potential, changing the pH, or producing surfactants, thereby increasing the bioavailability of DDT and promoting its absorption by bacteria. This synergistic degradation not only accelerates the decomposition of DDT but also achieves more complete mineralization compared to microbial activity alone. Bacterial strains that can degrade pesticides include 2,6-dichlorobenzamide (BAM) degrading Aminobacter spp., along with some other microbes that are employed for bioremediation. In this review, we aimed to explore the role of bacterial and fungal interactions in the enhanced degradation of DDT from the environment. Scientific literature was explored using online databases, and data from already published literature were retrieved. The key focus of the current study was to identify fungal-bacterial (FBI) interaction mechanisms to provide the basis for the eco-friendly degradation of harmful","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Segatella intestinalis sp. nov., and Parabacteroides caeci sp. nov., novel potential probiotics from the human gut microbiome","authors":"Md Shamsuzzaman,&nbsp;Ram Hari Dahal,&nbsp;Yoon-Jung Choi,&nbsp;Shukho Kim,&nbsp;Jungmin Kim","doi":"10.1007/s10482-025-02150-4","DOIUrl":"10.1007/s10482-025-02150-4","url":null,"abstract":"<div><p>Two bacterial strains, B2-R-102^T and W1-Q-101^T, were isolated from the feces of a healthy Korean individual. These strains were Gram-stain negative, anaerobic, and non-motile, growing optimally between 20 and 40 °C and at pH 5.5–8.0. Importantly, they survived at pH 2.0 and tolerated 0.3% bile salts and pepsin after a 4 h exposure. The strains demonstrated in vitro antioxidant activity, inhibiting DPPH radicals by 48.12 ± 1.45 and 70.80 ± 12.8%, respectively. Furthermore, they inhibited <i>α</i>-amylase activity by 46.52 ± 4.42 to 60.84 ± 2.20%, compared to 74.82 ± 0.76% inhibition by sitagliptin. In vitro, anti-inflammatory assays revealed 57.77 ± 3.15 to 62.39 ± 2.23% inhibition of albumin protein denaturation, comparable to aspirin 72 ± 2.39% inhibition. The abundant cellular fatty acids were C_15:0, C_16:0, iso-C_15:0, C_18:1<i>ω</i>9<i>c</i>_, anteiso-C_15:0 and iso-C_17:0 3-OH. Neither strain exhibited haemolytic activity, and genomic analysis revealed no acquired antibiotic resistance or virulence genes. Phylogenetic analysis showed that B2-R-102^T and W1-Q-101^T belonged to the genera <i>Segatella</i> and <i>Parabacteroides</i>, with 96.9 and 97.7% 16S rRNA gene sequence similarities to <i>Segatella copri</i> CB7^T and <i>Parabacteroides goldsteinii</i> DSM 19448^T, respectively. Furthermore, biosynthetic gene cluster analysis revealed the potential for antimicrobial thiopeptides, lanthipeptides, and non-ribosomal peptides (NRPs). In silico average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were below the thresholds to distinguish novel species. Based on phenotypic, genomic, and phylogenetic analysis, we propose the names <i>Segatella intestinalis</i> sp. nov. (type strain B2-R-102^T = CGMCC 1.17963^T = KCTC 25417^T) and <i>Parabacteroides caeci</i> sp<i>.</i> nov. (type strain W1-Q-101^T = KCTC 25456^T = CGMCC 1.17991^T).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144892425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic profiling of gut microbiome: associating their role with the advancement of diabetic nephropathy","authors":"Poornima Bhatt,&nbsp;Preeti Rajesh,&nbsp;Deepak Kukkar,&nbsp;Ki-Hyun Kim","doi":"10.1007/s10482-025-02141-5","DOIUrl":"10.1007/s10482-025-02141-5","url":null,"abstract":"<div><p>Emerging evidence suggests that alterations in the gut microbiome should play a critical role in the development and progression of type 2 diabetes and its complication such as diabetic nephropathy (DN). Nevertheless, a considerable gap remains in our understanding of the interconnection between DN pathogenesis and gut microbiota arrangement. In this context, this review highlights recent research on the connection between the intestinal microbiota and DN risk, with a particular focus on the role of microbial metabolites in disease development. It also highlights recent advancements in metagenomic analyses of gut microbial communities and their potential contribution to the progression of DN. Further, it describes the challenges associated with the metagenomics-based analysis of intestinal microbiota and the advancement of therapeutics for DN. An exploration of the surveyed literature reveals the lack of any definitive correlation between gut microbiota and DN transition, even when assessed in view of widespread geographical and ethnic diversity. Future research in this domain should be conducted to address various issues like increasing the number of participants, intake patient diversity, logistical difficulties, and racial disparities. A critical assessment of these parameters will help improve our understanding of the potential contribution of gut microbiota to the DN progression.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}