Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology最新文献_第5页

Unveiling tribal treasures: myco-chemical characterization and pharmacological evaluation of an unexplored Russula pers. species 揭开部落宝藏的神秘面纱：一种未开发的 Russula pers.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-10-05 DOI: 10.1007/s10482-024-02018-z

Gouri Basak, Soumitra Paloi, Arghya Naskar, Sandipta Ghosh, Krishnendu Acharya

{"title":"Unveiling tribal treasures: myco-chemical characterization and pharmacological evaluation of an unexplored Russula pers. species","authors":"Gouri Basak, Soumitra Paloi, Arghya Naskar, Sandipta Ghosh, Krishnendu Acharya","doi":"10.1007/s10482-024-02018-z","DOIUrl":"10.1007/s10482-024-02018-z","url":null,"abstract":"<div>During extensive field explorations of the Lateritic area in West Bengal, one remarkable wild Russuloid macrofungus, ethnically termed “Kend Patra,” was collected. The species was known to enrich the diet of the local people, being considered as income source for some tribal groups. Using morphological characters and molecular analysis of this collection, provide a unique placement of the taxon in the Russula subgenus Compactae (Fr.) Bon. Further in order to find functional constituents for biopharma applications, methanolic extract was prepared that shows the existence of a substantial amounts of phenol, flavonoid, ascorbic acid and carotenoids. Antioxidant activity was determined where the fraction demonstrated strong DPPH, ABTS, and nitric oxide radical scavenging activities, high Fe2+ ion chelating ability, and a reducing power with EC50 values ranging from 538.69 to 891.75 µg/ml. The extract was found to be effective against Listeria monocytogenes, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Staphylococcus aureus. In addition, the extract exhibited potent anticancer activities as it inhibited A549 cell proliferation, caused morphological changes, elevated ROS levels, hindered the clonogenic ability and migratory potential of cancerous cells, arrested cell cycle progression at S phase, and induced apoptosis by modulating the intrinsic mitochondrial pathway. Overall, this study contributes a new species to the world’s myco-diversity and presents an exciting opportunity for future researchers to conduct comprehensive investigations on this unique species in order to uncover potential new medications for human use.</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of adhesins (EcpD, FdeC, FimH) expressed in mammary pathogenic Escherichia coli on adhesion to bovine mammary epithelial cells 乳腺致病性大肠杆菌表达的粘附素（EcpD、FdeC、FimH）参与了牛乳腺上皮细胞的粘附。

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-10-03 DOI: 10.1007/s10482-024-02025-0

Yusaku Tsugami, Taketoshi Iwata, Aoi Sugiyama, Megumi Onishi, Kei-ichi Nakajima, Makoto Osaki, Yuya Nagasawa

{"title":"Involvement of adhesins (EcpD, FdeC, FimH) expressed in mammary pathogenic Escherichia coli on adhesion to bovine mammary epithelial cells","authors":"Yusaku Tsugami, Taketoshi Iwata, Aoi Sugiyama, Megumi Onishi, Kei-ichi Nakajima, Makoto Osaki, Yuya Nagasawa","doi":"10.1007/s10482-024-02025-0","DOIUrl":"10.1007/s10482-024-02025-0","url":null,"abstract":"<div>Mammary pathogenic Escherichia coli (MPEC) causes mastitis, which results in substantial economic losses to the dairy industry. A high percentage of Escherichia coli isolated from cows with clinical mastitis harbor adhesin genes, such as fimH. However, it is unclear whether these adhesins are important in the adhesion of MPEC to bovine mammary epithelial cells (BMECs). Therefore, we investigated the effect of adhesins (EcpD, FdeC, and FimH) in MPEC on adherence to the bovine mammary epithelium using cultured BMECs. For this purpose, we used wild-type MPEC as well as single- and double-mutants of fimH, ecpD, and fdeC, and performed adhesion assays with BMECs. First, BMECs were cultured in the presence of lactogenic hormones to induce milk component production and tight junction formation. The bacterial count of the wild-type strain that adhered to the BMECs increased in a dose-dependent manner. In deletion mutant strains, the ΔfimH strain showed lower adhesion (P < 0.05), whereas the adhesion ratio of the ΔecpD and ΔfdeC strains was not statistically different compared with that of the wild-type strain (P > 0.05). Additionally, the fimH/fdeC double-deletion mutants showed the lowest adhesion to BMECs. In conclusion, FimH is crucial in the adhesion of MPEC to BMECs. Overall, our work identifies FimH or FimH/FdeC as interesting targets for future drugs or vaccines to improve the treatment, prevention or chronicity of mastitis induced by MPEC.</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aquibaculum sediminis sp. nov., a halotolerant bacteria isolated from salt lake sediment Aquibaculum sediminis sp.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-10-01 DOI: 10.1007/s10482-024-02024-1

Qing-Yu Xu, Lei Gao, Dildar Wu, Xin-Yao Li, Yong-Hong Liu, Yao Zhang, Yue-Heng Chen, Ting-Ting She, Bao-Zhu Fang, Wen-Jun Li

{"title":"Aquibaculum sediminis sp. nov., a halotolerant bacteria isolated from salt lake sediment","authors":"Qing-Yu Xu, Lei Gao, Dildar Wu, Xin-Yao Li, Yong-Hong Liu, Yao Zhang, Yue-Heng Chen, Ting-Ting She, Bao-Zhu Fang, Wen-Jun Li","doi":"10.1007/s10482-024-02024-1","DOIUrl":"10.1007/s10482-024-02024-1","url":null,"abstract":"<div>An aerobic, Gram-stain negative bacterium was isolated from sediment samples of Barkol salt lake in Hami City, Xinjiang Uygur Autonomous Region, China, with the number EGI_FJ10229T. The strain is ellipse-shaped, oxidase-negative, catalase-positive, and has white, round, smooth, opaque colonies on marine 2216 E agar plate. Growth occurs at 4.0–37.0 ℃ (optimal:30.0 ℃), pH 7.0–9.0 (optimal: pH 8.0) and NaCl concentration of 0–8.0% (optimal: 3.0%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that the isolated strain should be assigned to the genus Aquibaculum and was most closely related to Aquibaculum arenosum CAU 1616 T. Average nucleotide identity (ANI) and Average amino-acid identity (AAI) values between the type species of the genus Aquibaculum and other related type species were lower than the threshold values recommended for bacterial species. The genomic DNA G + C content of EGI_FJ10229T was 65.41%. The major polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, phosphatidylethanolamine and unidentified phospholipid. The major fatty acids (> 5%) were C19:0 cyclo ω8c (42.0%) and C18:1 ω7c (33.78%). The respiratory quinone identified was Q-10. Differential phenotypic and genotypic characteristics of this strain and species of genus Aquibaculum showed that the strain should be classified as representing a new species belonging to this genus, for which the name Aquibaculum sediminis sp. nov. is proposed. The type strain of the proposed novel species is EGI_FJ10229T (= KCTC 8570 T = GDMCC 1.4598 T).</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Roseobacter weihaiensis sp. nov., a cellulose-degrading bacterium isolated from intestinal content of Nipponacmea schrenckii collected from golden beach in Weihai, China 从中国威海金沙滩采集的日本石斑鱼肠道内容物中分离出的纤维素降解细菌--威海玫瑰杆菌（Roseobacter weihaiensis sp.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-28 DOI: 10.1007/s10482-024-02009-0

Yu-Rui Li, Ming-Jing Zhang, Yan-Jun Yi, Xiao-Chen Wang, Dong-Mei San, Yan-Xia Zhou

{"title":"Roseobacter weihaiensis sp. nov., a cellulose-degrading bacterium isolated from intestinal content of Nipponacmea schrenckii collected from golden beach in Weihai, China","authors":"Yu-Rui Li, Ming-Jing Zhang, Yan-Jun Yi, Xiao-Chen Wang, Dong-Mei San, Yan-Xia Zhou","doi":"10.1007/s10482-024-02009-0","DOIUrl":"10.1007/s10482-024-02009-0","url":null,"abstract":"<div>A Gram-staining-negative, dark pink, rod-shaped, amastigote and cellulose-degrading strain, designated H9T, was isolated from intestinal contents of Nipponacmea schrenckii. The isolate was able to grow at 4–42 °C (optimum, 25 °C), at pH 6.5–9.0 (optimum, pH 7.0), and with 0.0–11.0% (w/v) NaCl (optimum, 3.0–5.0%). Phylogenetic analysis of the 16S rRNA gene sequence suggested that isolate H9T belongs to the genus Roseobacter, neighboring Roseobacter insulae YSTF-M11T, Roseobacter cerasinus AI77T and Roseobacter ponti MM-7 T, and the pairwise sequence showed the highest similarity of 99.1% to Roseobacter insulae YSTF-M11T. The major fatty acid was summed feature 8 (C18:1ω7c and/or C18:1ω6c; 81.08%). The predominant respiratory quinone was Q-10. The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, an unknown lipid, and a small amount of an unknown phospholipid. The genome of strain H9T was 5,351,685 bp in length, and the DNA G + C content was 59.8%. The average amino acid identity (AAI), average nucleotide identity (ANI), and digital DNA hybridization (dDDH) values between strain H9T and closely related strains were 63.4–76.8%, 74.7–78.8%, and 13.4–19.7%, respectively. On the basis of the phenotypic, chemical taxonomic, and phylogenetic data, it is suggested that strain H9T should represent a novel species in the genus Roseobacter, for which the name Roseobacter weihaiensis sp. nov. is proposed. The type strain is H9T (= KCTC 82507 T = MCCC 1K04354T).</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Florfenicol-resistant Brevundimonas sanguinis sp. nov., a novel bacterium isolated from patient blood in South Korea 从韩国患者血液中分离出的一种新型细菌--耐氟苯尼考的Brevundimonas sanguinis sp.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s10482-024-02020-5

Ji Hee Lee, Yewon An, Su Yeon Kim

{"title":"Florfenicol-resistant Brevundimonas sanguinis sp. nov., a novel bacterium isolated from patient blood in South Korea","authors":"Ji Hee Lee, Yewon An, Su Yeon Kim","doi":"10.1007/s10482-024-02020-5","DOIUrl":"10.1007/s10482-024-02020-5","url":null,"abstract":"<div>An aerobic, Gram-stain-negative, non-motile, non-spore-forming, short rod-shaped bacterial strain, designated NCCP 15609 T, was isolated from the blood sample of a patient in the Republic of Korea. The strain was identified as Brevundimonas diminuta using MALDI–TOF. A phylogenetic tree constructed using 16S rRNA gene sequences revealed that the isolate was of the genus Brevundimonas with 99.8% similarity to B. naejangsanensis. The strain NCCP 15609T genome consisted of one contig with 3,063,090 bp, and had a G+C content of 67.4%. The genome contained 2,949 protein-coding sequences, 52 tRNAs, and 6 rRNAs. The DNA–DNA hybridisation between NCCP 15609T and B. naejangsanensis yielded 92.5% and 49.5% ± 2.6%, respectively, using the average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH). The predominant fatty acids of strain NCCP 15609T were summed feature 8 (C18:1 ω7c/C18:1 ω6c) and C16:0. The isolate contained polar lipids and quinone, corresponding to phosphatidylglycerol, 1,2-di-O-acyl-3-O-[D-glycopyranosyl (1 → 4)-α-D-glucopyranuronosyl] glycerol, and ubiquinone-10, respectively. Based on its phylogenetic, physiological, and chemotaxonomic characteristics, we suggest that NCCP 15609T represents a novel pathogen resource of the genus Brevundimonas and propose to name it Brevundimonas sanguinis sp. nov. The type strain is NCCP 15609T (= DSM 116005T).</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acidisoma cladoniae sp. nov., an acidotolerant bacterium isolated from an Antarctic lichen Acidisoma cladoniae sp.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-25 DOI: 10.1007/s10482-024-02021-4

Un Cho, Jehyun Jeon, Woohyun Kim, Soon Gyu Hong, Hyoungseok Lee, Yung Mi Lee

{"title":"Acidisoma cladoniae sp. nov., an acidotolerant bacterium isolated from an Antarctic lichen","authors":"Un Cho, Jehyun Jeon, Woohyun Kim, Soon Gyu Hong, Hyoungseok Lee, Yung Mi Lee","doi":"10.1007/s10482-024-02021-4","DOIUrl":"10.1007/s10482-024-02021-4","url":null,"abstract":"<div>Gram-staining-negative, aerobic, white-cream-pearly colony, coccobacilli, and non-motile bacterial strain, PAMC 29798T was isolated from an Antarctic lichen. The strain was acidotolerant and psychrotolerant growing at pH 4.0–7.5 (optimally at pH 4.0–6.5) and 0–25 °C (optimally at 10–20 °C). The major fatty acids are Summed Feature 8, C18:1 2OH, and C19:0 cyclo ω8c. The major respiratory quinone was Q-10. Phylogenetic and phylogenomic analyses indicated that strain PAMC 29798T belonged to the genus Acidisoma and 16S rRNA gene sequences of PAMC 29798T were closely related to Acidisoma silvae (97.7% sequence similarity), Acidisoma cellulosilyticum (96.5%), Acidisoma tundrae (96.5%), and Acidisoma sibiricum (96.3%). Genomic relatedness analyses showed that strain PAMC 29798T was clearly distinguished from type strains of the genus Acidisoma based on values of average nucleotide identity (< 75%) and the digital DNA-DNA hybridization (< 19.6%). Genome analysis revealed that the genome size of PAMC 29798T is approximately 5.0 Mb with a G+C content of 63.4%. The complete genome comprises 5 contigs containing 4636 protein-coding genes, 46 tRNA genes, and 2 rRNA operons. The genome possesses genes for light-harvesting complexes, type-II photosynthetic reaction center, and C-P lyase to solubilize organic phosphates, while genes encoding nitrogenase iron protein involved in the nitrogen fixation were not present. Based on the results of phylogenetic, genome-based relatedness, and physiological and genomic analyses, strain PAMC 29798T is proposed to represent a novel species of the genus Acidisoma, with the name Acidisoma cladoniae. The type strain is PAMC 29798T (= KCTC 82159T = JCM 35634T).</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thiobacillus sedimenti sp. nov., a chemolithoautotrophic sulphur-oxidizing bacterium isolated from freshwater sediment 一种从淡水沉积物中分离出来的化学溶解自养型硫氧化细菌 Thiobacillus sedimenti sp.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-24 DOI: 10.1007/s10482-024-02026-z

Chenming Dai, Guangye Zhang, Weitie Lin, Jianfei Luo

{"title":"Thiobacillus sedimenti sp. nov., a chemolithoautotrophic sulphur-oxidizing bacterium isolated from freshwater sediment","authors":"Chenming Dai, Guangye Zhang, Weitie Lin, Jianfei Luo","doi":"10.1007/s10482-024-02026-z","DOIUrl":"10.1007/s10482-024-02026-z","url":null,"abstract":"<div>A sulphur-oxidizing bacterium, designated strain SCUT-2T, was isolated from freshwater sediment collected from the Pearl River in Guangzhou, PR China. This strain was an obligate chemolithoautotroph, utilizing reduced sulphur compounds (elemental sulphur, thiosulphate, tetrathionate and sulphite) as the electron donor. Growth of strain SCUT-2T was observed at 20–40 ℃ (optimum at 30 °C), pH 5.0–9.0 (optimum at 6.0), and NaCl concentration range of 0–9 g L−1 (optimum at 1 g L−1). The major cellular fatty acids were C16:0 ω7c and cyclo-C17:0. The DNA G + C content of the complete genome sequence was 66.8 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCUT-2T formed a lineage within the genus Thiobacillus, showing gene sequence identity of 98.0% with its closest relative Thiobacillus thioparus THI 115. The genome of strain SCUT-2T contains multiple genes encoding sulphur-oxidizing enzymes that catalyse the oxidation of reduced sulphur compounds, partial genes that are necessary for denitrification, and the genes encoding cbb3-type cytochrome c oxidase, aa3-type cytochrome c oxidase and bd-type quinol oxidase. Facultative anaerobic growth occurs when using nitrate as the electron acceptor and thiosulphate as the electron donor. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic analysis, strain SCUT-2T (= GDMCC 1.4108T = JCM 39443T) is deemed to represent a novel Thiobacillus species, for which we propose the name Thiobacillus sedimenti sp. nov.</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142309005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paralabilibaculum antarcticum gen. nov., sp. nov., an anaerobic marine bacterium of the family Marinifilaceae isolated from Antarctica sea ice Paralabilibaculum antarcticum gen.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-21 DOI: 10.1007/s10482-024-02022-3

Yifan Zhuang, Yunxiao Zhang, Wei Dai, Yantao Liang, Xiaoyu Yang, Yaru Wang, Xiaochong Shi, Xiao-Hua Zhang

{"title":"Paralabilibaculum antarcticum gen. nov., sp. nov., an anaerobic marine bacterium of the family Marinifilaceae isolated from Antarctica sea ice","authors":"Yifan Zhuang, Yunxiao Zhang, Wei Dai, Yantao Liang, Xiaoyu Yang, Yaru Wang, Xiaochong Shi, Xiao-Hua Zhang","doi":"10.1007/s10482-024-02022-3","DOIUrl":"10.1007/s10482-024-02022-3","url":null,"abstract":"<div>A novel bacterial strain, designated DW002T, was isolated from the sea ice of Cape Evans, McMurdo Sound, Antarctica. Cells of the strain were Gram-negative, obligate anaerobic, motile, non-flagellated, and short rod-shaped. The strain DW002T grew at 4–32 ℃ (optimum at 22–28 ℃) and thrived best at pH 7.0, NaCl concentration of 2.5% (w/v). The predominant isoprenoid quinone of strain DW002T was menaquinone-7 (MK-7). The major fatty acids (> 10%) of DW002T were iso-C15:0, anteiso-C15:0 and iso-C17:1ω9c. The predominant polar lipids of strain DW002T contained two phosphatidylethanolamines, one unidentified glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G + C content of the strain DW002T was 34.8%. Strain DW002T encoded 237 carbohydrate-active enzymes. The strain DW002T had genes associated with dissimilatory nitrate reduction and assimilatory sulfate reduction metabolic pathways. Based on distinct physiological, chemotaxonomic, genome analysis and phylogenetic differences compared to other members of the phylogenetically related genera in the family Marinifilaceae, strain DW002T is proposed to represent a novel genus within the family. Therefore, the name Paralabilibaculum antarcticum gen. nov., sp. nov. is proposed. The type strain is DW002T (=KCTC 25274T=MCCC 1K06067T).</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteus faecis: a potentially pathogenic bacterium isolated from the freshwater Yangtze finless porpoise 粪变形杆菌：从淡水长江江豚体内分离出的潜在致病细菌。

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-21 DOI: 10.1007/s10482-024-02023-2

Richard William McLaughlin, YaLu Wang, ShuYa Zhang, HaiXia Xie, XiaoLing Wan, Hui Liu, YuJiang Hao, ChaoQun Wang, JinSong Zheng

{"title":"Proteus faecis: a potentially pathogenic bacterium isolated from the freshwater Yangtze finless porpoise","authors":"Richard William McLaughlin, YaLu Wang, ShuYa Zhang, HaiXia Xie, XiaoLing Wan, Hui Liu, YuJiang Hao, ChaoQun Wang, JinSong Zheng","doi":"10.1007/s10482-024-02023-2","DOIUrl":"10.1007/s10482-024-02023-2","url":null,"abstract":"<div>Proteus faecis is a gram-negative facultative anaerobic rod-shaped bacterium capable of swarming motility. It has been isolated from numerous sources such as humans, animals, and refuse and is considered potentially pathogenic towards humans. In this study, bacteria were isolated from the blowhole of a Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis; YFP) living in captivity in China. One bacterium, P. faecis porpoise, was isolated and whole genome sequencing done. Biofilm formation, motility and antimicrobial resistance were also investigated. To find putative virulence factors, the genome of P. faecis strain porpoise was compared to the genomic sequences of eight other P. faecis isolates using the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) (https://www.bv-brc.org/). The goal of this study was to initially characterize the pathogenicity of this bacterium isolated from a cetacean species using both pathogenomics and conventional approaches.</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142300084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential 基于基因组的福州单胞菌（Sphingomonas fuzhouensis sp.

IF 1.8 3区生物学

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-18 DOI: 10.1007/s10482-024-02017-0

Ling Yao, Guo-Hong Liu, Shu-Yi Zhang, Peng Gao, Christopher Rensing, Qiu-E Yang, Shun-Gui Zhou

{"title":"Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential","authors":"Ling Yao, Guo-Hong Liu, Shu-Yi Zhang, Peng Gao, Christopher Rensing, Qiu-E Yang, Shun-Gui Zhou","doi":"10.1007/s10482-024-02017-0","DOIUrl":"10.1007/s10482-024-02017-0","url":null,"abstract":"<div>Two facultatively aerobic strains, designated SGZ-02T and SGZ-792T, were isolated from plant Pennisetum sp., exhibiting the highest 16S rRNA gene sequence similarities with the type strains of Sphingomonas zeae LMG 28739T (98.6%) and Massilia forsythiae NBRC 114511T (98.4%), respectively. SGZ-02T grew between 5 and 45 °C, pH 5.0–11.0 and tolerated NaCl concentrations of 0–4% (w/v), whereas SGZ-792T thrived at 5–40 °C, pH 5.0–11.0 and NaCl tolerance to 0–3.5% (w/v). The major quinone of SGZ-02T was ubiquinone-10, with the dominant fatty acids being C16:0 (13.5%), Summed Feature 3 (6.3%), C14:02-OH (5.3%) and Summed Feature 8 (66.3%). SGZ-792T predominantly contained ubiquinone-8, with major fatty acids being C16:0 (20.3%), Summed Feature 3 (5.0%) and Summed Feature 8 (54.7%). Average nucleotide identity and digital DNA–DNA hybridization values between two strains and their closest references strains were below the bacterial species threshold. Based on genotypic and phenotypic characteristics, strains SGZ-02T and SGZ-792T are proposed as novel species within the genera Sphingomonas and Massilia, respectively. The suggested names for the new species are Sphingomonas fuzhouensis sp. nov. (SGZ-02T = GDMCC 1.4033T = JCM 36769T) and Massilia phyllosphaerae sp. nov. (SGZ-792T = GDMCC 1.4211T = JCM 36643T), respectively.</div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142254697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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